RchiOBHm_Chr5g0028941

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000066
Physical Location & Seq
Forward (+)
22728413 .. 22730979
2567 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30835

Sequence Viewer

Length: 2325 bp
ATGGCCTTTTTACACTGGCGGATGCAAATGATGAAACTCTCTTTGGTGATATTATTGGCAGCTGTAACAACAAGAGCAGCAGCTGATCAAGCCCTACATGGTTGTGCAGAAAAGTGCGGTGATATAACAATCCCATATCCATTTGGGATAGGCGATGGCTGCTACCTGCGTCCAGAATTCAACATCACTTGTAATGGACCCACCACACCCACGTCAACAAACTTTGCAAACAGTACCTATACTTTTCCCATGGCCATAGCTAACTTTTCCATTTCTGAGGGTGAGCTGCAAGTCATGCAGGCCGTAGCCCTAGATTGTTATGACAACAAGGGTGTAAAGATAGCCAACAGCACAAGTACGCTGGAGCTGCCGCCTTCTTATACCATCTCTGCAAAAAACAAGTTCTTTCTCGTTGGCTGTAATAATGCAGCAATTTATGAAGGTCTACTTCAGTTCCCAGACCCAGATGAAGGAAGTGACTTCCAAGCAGCCGGGTTCACTGTGACCTTGTGTGTAAATGAGCTTGGCAAAGAGTTGCACGAGTCTTGTGACGGCTTTGGGTGTGCCCAAACTTCCATCCTTAGTGGACTCCAAAATATCACTGTGTCCTTGGATGCACTTGATGCTGATAACCGAACAGATTCGTGGAGCTTAAAGTACCTGTGCAGCTACGCATTCATTGTGGACGAAGGCAATTTCACATTCTCCCCGGAGAGAAGTTTCCAACAATTGAATACCACAAGGCAGCAACTTCCAGTACTTATTAACTGGGCGATTGGGGGCGAGCCATGTGAAGTAGCAAAGAAGAACCACAGTACTTATGCATGCAAAGAAAATACCACCTGTGTCGACCGAAGTACCATCACAAATGGTACGGTTGGTTACCTTTGCCACTGCTTGCCAGGTTACCATGGAAATCCGTACCTTGGTTGCCAAGATATCGATGAGTGCCAGGCTTCAACTAACCCCTGCCAGAATGGGAAGTGCATAAACTCACCTCCAGGAAATTATTCTTGTCAATGTAACGGAGGGTACAAAAACAAAGATCCAATAACTTGCGTTGAATATCCCAAAGCAAAGAACACTTCCCTGAAAATTTCATTGGGTCTCATTGTAGGATTCTTGGTTTTAGTTGTTTCAATGTCATGGATATATTGGAAAATGAACAAAAGAAGGCTCATCAAACTCAAAGACAAGTACTTTAAAGAAAATGGCGGCTTAACATTACAGCAACGGCGCCTAGCTACTCATGGAGGAGGAGTGGAGACAACAAAAATCTTTACGGCTGAAGAGCTTAAGAAGGCAACGAACAATTATAATGCAGGTGAAATTCTTGGTGAAGGAGGCTACGGAACAGTTTACAAAGGAATATTGCCTGATAAAAGAGTTGTAGCCATCAAAAAGTCAAAACTGGGTGCCCCAACTCAAAGTGATCAATTTGTCAATGAGGTGACTATTCTTTCTCAAATCAACCATAGAAACGTGGTAAAATTATTAGGTTGTTGTTTCGAGACCGAAGTGCCTTTACTAGTATACGAGTACATCACCGGTGGAACTCTTTCTGAGCATGTATTCAAGAAAAGACAAAGATCTGTGCTTTCATGGGAATTACGACTGAAGATAGCATCAGAAACGGCAGGGGCACTTGCATACTTGCACTCCTCTACTTCCACTCCAATCATACATCGAGACGTGAAATCAACGAATATATTATTGGATGACAATTACACGGCCAAAGTGTCAGACTTTGGAGCTTCACGTTTCATTCCTATAGATCAAACTCAAGTAGCAACACTAGTGCAAGGGACACTTGGATACTTGGACCCTGAATACTGCCATTCAAACCAACTGACTGAAAAGAGTGATGTATATAGCTTTGGTGTTGTGCTAGCGGAGCTACTAACGACGAAAGTGGCAGTTTCTTTTGCTAGGCCCGAGGAAGAGAGAAACCTAGCAAGTTTGTTTATTTCTTCAATGGAAAAAGAATGCTTGAATCAAATTCTTGATGCTGAAATTCTGAATGAGAGGAATATGGAGACAATACGGCAAGTGGCGAATCTGGCGAAAAGATGTTTGAGGATAAAAGGAGAGGAAAGGCCTTTGATGAAAGAAGTAGCTATGGAGTTGGAGGGGATGAGAATTGAGGCAAAACATCCATGGAAATGTGGTAGTTCCTGCTCTGAGGGGACTGAGTACCTGTTAGGGTCATCCAATTCGAAGACTAATGTTGCGGATACTAAAGGTGAAGGTGTTTCTAGTGGTACGACCAGCGGGTATGCCAGTATGCAAATTCCGATGTTAATGTCACATGAACATGGGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

774

Amino Acids

85.15

Weight (kDa)

6.16

Isoelectric Point (pI)

31.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 97 1.6e-09 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 313 - 353 3.4e-06 Calcium-binding EGF domain
Pkinase PF00069 439 - 705 3.6e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 442 - 709 1.7e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1317
AarI CACCTGC 1 cut(s) 1313
Acc36I ACCTGC 2 cut(s) 174, 1313
AccB1I GGYRCC 2 cut(s) 1236, 1415
AccI GTMKAC 3 cut(s) 445, 849, 1533
AciI CCGC 7 cut(s) 19, 117, 371, 1215, 1892, 2231, 2271
AclWI GGATC 1 cut(s) 1040
AcoI YGGCCR 2 cut(s) 252, 1731
AcsI RAATTY 6 cut(s) 176, 1095, 1329, 1998, 2013, 2289
AcuI CTGAAG 3 cut(s) 434, 1308, 1637
AcyI GRCGYC 1 cut(s) 1237
AfiI CCNNNNNNNGG 2 cut(s) 470, 926
AflII CTTAAG 1 cut(s) 1295
AgeI ACCGGT 1 cut(s) 1547
AgsI TTSAA 9 cut(s) 181, 733, 960, 1064, 1140, 1576, 1842, 1974, 1993
AhlI ACTAGT 2 cut(s) 1528, 1795
AjiI CACGTC 2 cut(s) 213, 1693
AjnI CCWGG 3 cut(s) 901, 951, 1000
AjuI GAANNNNNNNTTGG 6 cut(s) 26, 58, 1085, 1117, 1697, 1729
Alw26I GTCTC 5 cut(s) 1112, 1259, 1505, 1683, 2030
AlwI GGATC 1 cut(s) 1040
AlwNI CAGNNNCTG 1 cut(s) 83
Ama87I CYCGRG 1 cut(s) 1934
AoxI GGCC 6 cut(s) 3, 252, 300, 1731, 1931, 2096
ApoI RAATTY 6 cut(s) 176, 1095, 1329, 1998, 2013, 2289
AsiGI ACCGGT 1 cut(s) 1547
Asp700I GAANNNNTTC 3 cut(s) 640, 1009, 1558
AspLEI GCGC 1 cut(s) 1239
AspS9I GGNCC 3 cut(s) 197, 1822, 1932
AsuC2I CCSGG 2 cut(s) 493, 710
AsuHPI GGTGA 9 cut(s) 58, 131, 293, 987, 1337, 1349, 1462, 1537, 2255
AsuII TTCGAA 1 cut(s) 2216
AsuNHI GCTAGC 1 cut(s) 1888
AvaI CYCGRG 1 cut(s) 1934
AvaII GGWCC 2 cut(s) 197, 1822
BaeGI GKGCMC 3 cut(s) 568, 1420, 1645
BaeI ACNNNNGTAYC 2 cut(s) 841, 874
BalI TGGCCA 1 cut(s) 254
BanI GGYRCC 2 cut(s) 1236, 1415
BarI GAAGNNNNNNTAC 2 cut(s) 1509, 1541
BauI CACGAG 1 cut(s) 539
BbsI GAAGAC 1 cut(s) 2225
BccI CCATC 5 cut(s) 149, 392, 584, 869, 1403
BceAI ACGGC 7 cut(s) 287, 568, 1250, 1299, 1650, 1746, 2060
BciT130I CCWGG 3 cut(s) 903, 953, 1002
BciVI GTATCC 2 cut(s) 1808, 2227
BclI TGATCA 2 cut(s) 85, 1432
BcnI CCSGG 2 cut(s) 493, 710
BcoDI GTCTC 5 cut(s) 1112, 1259, 1505, 1683, 2030
BcuI ACTAGT 2 cut(s) 1528, 1795
BfaI CTAG 8 cut(s) 311, 1241, 1529, 1796, 1889, 1929, 1952, 2256
BfmI CTRYAG 1 cut(s) 1770
BfoI RGCGCY 1 cut(s) 1240
BfrI CTTAAG 1 cut(s) 1295
BfuAI ACCTGC 2 cut(s) 174, 1313
BfuI GTATCC 2 cut(s) 1808, 2227
BglII AGATCT 1 cut(s) 1589
BmcAI AGTACT 3 cut(s) 759, 817, 1199
Bme1390I CCNGG 5 cut(s) 493, 710, 903, 953, 1002
Bme18I GGWCC 2 cut(s) 197, 1822
BmeT110I CYCGRG 1 cut(s) 1934
BmgBI CACGTC 2 cut(s) 213, 1693
BmgT120I GGNCC 3 cut(s) 197, 1822, 1932
BmiI GGNNCC 4 cut(s) 199, 1238, 1417, 1824
BmrFI CCNGG 5 cut(s) 493, 710, 903, 953, 1002
BmrI ACTGGG 2 cut(s) 778, 1421
BmsI GCATC 5 cut(s) 12, 604, 613, 1634, 1996
BmtI GCTAGC 1 cut(s) 1892
BmuI ACTGGG 2 cut(s) 778, 1421
BpiI GAAGAC 1 cut(s) 2225
BpmI CTGGAG 2 cut(s) 383, 984
Bpu14I TTCGAA 1 cut(s) 2216
BpuEI CTTGAG 1 cut(s) 1767
BpuMI CCSGG 2 cut(s) 493, 710
Bsa29I ATCGAT 1 cut(s) 942
BsaHI GRCGYC 1 cut(s) 1237
BsaI GGTCTC 2 cut(s) 1112, 1505
BsaJI CCNNGG 7 cut(s) 249, 609, 708, 910, 925, 1935, 2156
BsaWI WCCGGW 1 cut(s) 1547
BsaXI ACNNNNNCTCC 6 cut(s) 1245, 1275, 1643, 1657, 1673, 1687
Bsc4I CCNNNNNNNGG 2 cut(s) 470, 926
Bse118I RCCGGY 1 cut(s) 1547
Bse1I ACTGG 5 cut(s) 20, 755, 773, 1416, 2280
BseBI CCWGG 3 cut(s) 903, 953, 1002
BseCI ATCGAT 1 cut(s) 942
BseDI CCNNGG 7 cut(s) 249, 609, 708, 910, 925, 1935, 2156
BseGI GGATG 7 cut(s) 27, 576, 619, 1723, 2139, 2152, 2207
BseLI CCNNNNNNNGG 2 cut(s) 470, 926
BseMII CTCAG 4 cut(s) 267, 1554, 2172, 2181
BseNI ACTGG 5 cut(s) 20, 755, 773, 1416, 2280
BseRI GAGGAG 3 cut(s) 1269, 1272, 1651
BseSI GKGCMC 3 cut(s) 568, 1420, 1645
BsgI GTGCAG 2 cut(s) 126, 685
Bsh1285I CGRYCG 1 cut(s) 853
BshFI GGCC 6 cut(s) 5, 254, 302, 1733, 1933, 2098
BshNI GGYRCC 2 cut(s) 1236, 1415
BshTI ACCGGT 1 cut(s) 1547
BshVI ATCGAT 1 cut(s) 942
BsiEI CGRYCG 1 cut(s) 853
BsiHKCI CYCGRG 1 cut(s) 1934
BsiSI CCGG 3 cut(s) 492, 710, 1548
BslFI GGGAC 2 cut(s) 1819, 2200
BslI CCNNNNNNNGG 2 cut(s) 470, 926
BsmAI GTCTC 5 cut(s) 1112, 1259, 1505, 1683, 2030
BsmBI CGTCTC 1 cut(s) 1683
BsmFI GGGAC 2 cut(s) 1819, 2200
BsmI GAATGC 2 cut(s) 674, 1991
BsnI GGCC 6 cut(s) 5, 254, 302, 1733, 1933, 2098
Bso31I GGTCTC 2 cut(s) 1112, 1505
BsoBI CYCGRG 1 cut(s) 1934
Bsp119I TTCGAA 1 cut(s) 2216
Bsp1286I GDGCHC 3 cut(s) 568, 1420, 1645
Bsp143I GATC 5 cut(s) 85, 1045, 1432, 1589, 1774
Bsp19I CCATGG 3 cut(s) 249, 910, 2156
BspACI CCGC 7 cut(s) 19, 117, 371, 1215, 1892, 2231, 2271
BspANI GGCC 6 cut(s) 5, 254, 302, 1733, 1933, 2098
BspCNI CTCAG 4 cut(s) 268, 1555, 2173, 2182
BspDI ATCGAT 1 cut(s) 942
BspLI GGNNCC 4 cut(s) 199, 1238, 1417, 1824
BspMI ACCTGC 2 cut(s) 174, 1313
BspOI GCTAGC 1 cut(s) 1892
BspPI GGATC 1 cut(s) 1040
BspQI GCTCTTC 1 cut(s) 1284
BspT104I TTCGAA 1 cut(s) 2216
BspT107I GGYRCC 2 cut(s) 1236, 1415
BspTI CTTAAG 1 cut(s) 1295
BspTNI GGTCTC 2 cut(s) 1112, 1505
BsrFI RCCGGY 1 cut(s) 1547
BsrI ACTGG 5 cut(s) 20, 755, 773, 1416, 2280
BssAI RCCGGY 1 cut(s) 1547
BssECI CCNNGG 7 cut(s) 249, 609, 708, 910, 925, 1935, 2156
BssMI GATC 5 cut(s) 85, 1045, 1432, 1589, 1774
BssNAI GTATAC 1 cut(s) 1534
BssNI GRCGYC 1 cut(s) 1237
BssSI CACGAG 1 cut(s) 539
BssT1I CCWWGG 5 cut(s) 249, 609, 910, 925, 2156
Bst1107I GTATAC 1 cut(s) 1534
Bst2BI CACGAG 1 cut(s) 539
Bst2UI CCWGG 3 cut(s) 903, 953, 1002
Bst4CI ACNGT 6 cut(s) 233, 502, 604, 815, 877, 1357
Bst6I CTCTTC 2 cut(s) 1284, 1935
BstACI GRCGYC 1 cut(s) 1237
BstAFI CTTAAG 1 cut(s) 1295
BstAPI GCANNNNNTGC 2 cut(s) 295, 623
BstBI TTCGAA 1 cut(s) 2216
BstC8I GCNNGC 5 cut(s) 300, 785, 826, 899, 1890
BstDEI CTNAG 5 cut(s) 276, 581, 1563, 2181, 2190
BstDSI CCRYGG 3 cut(s) 249, 910, 2156
BstEII GGTNACC 2 cut(s) 881, 905
BstF5I GGATG 7 cut(s) 27, 576, 619, 1723, 2139, 2152, 2207
BstH2I RGCGCY 1 cut(s) 1240
BstHHI GCGC 1 cut(s) 1239
BstKTI GATC 5 cut(s) 88, 1048, 1435, 1592, 1777
BstMAI GTCTC 5 cut(s) 1112, 1259, 1505, 1683, 2030
BstMBI GATC 5 cut(s) 85, 1045, 1432, 1589, 1774
BstMCI CGRYCG 1 cut(s) 853
BstMWI GCNNNNNNNGC 7 cut(s) 89, 159, 295, 367, 623, 1894, 2060
BstNI CCWGG 3 cut(s) 903, 953, 1002
BstNSI RCATGY 2 cut(s) 828, 1571
BstPI GGTNACC 2 cut(s) 881, 905
BstSCI CCNGG 5 cut(s) 491, 708, 901, 951, 1000
BstSFI CTRYAG 1 cut(s) 1770
BstSLI GKGCMC 3 cut(s) 568, 1420, 1645
BstV2I GAAGAC 1 cut(s) 2225
BstX2I RGATCY 2 cut(s) 1045, 1589
BstYI RGATCY 2 cut(s) 1045, 1589
BstZ17I GTATAC 1 cut(s) 1534
Bsu15I ATCGAT 1 cut(s) 942
BsuI GTATCC 2 cut(s) 1808, 2227
BsuRI GGCC 6 cut(s) 5, 254, 302, 1733, 1933, 2098
BsuTUI ATCGAT 1 cut(s) 942
BtgI CCRYGG 3 cut(s) 249, 910, 2156
BtgZI GCGATG 1 cut(s) 168
BtrI CACGTC 2 cut(s) 213, 1693
BtsCI GGATG 7 cut(s) 27, 576, 619, 1723, 2139, 2152, 2207
BtsI GCAGTG 1 cut(s) 892
BtsIMutI CAGTG 4 cut(s) 13, 498, 600, 892
BveI ACCTGC 2 cut(s) 174, 1313
Cac8I GCNNGC 5 cut(s) 300, 785, 826, 899, 1890
CaiI CAGNNNCTG 1 cut(s) 83
CfoI GCGC 1 cut(s) 1239
Cfr10I RCCGGY 1 cut(s) 1547
Cfr13I GGNCC 3 cut(s) 197, 1822, 1932
ClaI ATCGAT 1 cut(s) 942
CseI GACGC 1 cut(s) 158
CspAI ACCGGT 1 cut(s) 1547
DdeI CTNAG 5 cut(s) 276, 581, 1563, 2181, 2190
DinI GGCGCC 1 cut(s) 1238
DpnI GATC 5 cut(s) 87, 1047, 1434, 1591, 1776
DpnII GATC 5 cut(s) 85, 1045, 1432, 1589, 1774
DraI TTTAAA 1 cut(s) 1204
EaeI YGGCCR 2 cut(s) 252, 1731
Eam1104I CTCTTC 2 cut(s) 1284, 1935
EarI CTCTTC 2 cut(s) 1284, 1935
EciI GGCGGA 1 cut(s) 34
Eco130I CCWWGG 5 cut(s) 249, 609, 910, 925, 2156
Eco147I AGGCCT 1 cut(s) 2098
Eco31I GGTCTC 2 cut(s) 1112, 1505
Eco32I GATATC 1 cut(s) 940
Eco47I GGWCC 2 cut(s) 197, 1822
Eco57I CTGAAG 3 cut(s) 434, 1308, 1637
Eco88I CYCGRG 1 cut(s) 1934
Eco91I GGTNACC 2 cut(s) 881, 905
EcoO65I GGTNACC 2 cut(s) 881, 905
EcoRI GAATTC 1 cut(s) 176
EcoRII CCWGG 3 cut(s) 901, 951, 1000
EcoRV GATATC 1 cut(s) 940
EcoT14I CCWWGG 5 cut(s) 249, 609, 910, 925, 2156
EcoT22I ATGCAT 1 cut(s) 826
EgeI GGCGCC 1 cut(s) 1238
EheI GGCGCC 1 cut(s) 1238
ErhI CCWWGG 5 cut(s) 249, 609, 910, 925, 2156
Esp3I CGTCTC 1 cut(s) 1683
FalI AAGNNNNNCTT 4 cut(s) 432, 464, 1794, 1826
FaqI GGGAC 2 cut(s) 1819, 2200
FauI CCCGC 1 cut(s) 2264
FbaI TGATCA 2 cut(s) 85, 1432
FblI GTMKAC 3 cut(s) 445, 849, 1533
FokI GGATG 7 cut(s) 34, 563, 626, 1730, 2139, 2146, 2194
FspBI CTAG 8 cut(s) 311, 1241, 1529, 1796, 1889, 1929, 1952, 2256
GlaI GCGC 1 cut(s) 1238
GsuI CTGGAG 2 cut(s) 383, 984
HaeII RGCGCY 1 cut(s) 1240
HaeIII GGCC 6 cut(s) 5, 254, 302, 1733, 1933, 2098
HapII CCGG 3 cut(s) 492, 710, 1548
HgaI GACGC 1 cut(s) 158
HhaI GCGC 1 cut(s) 1239
Hin1I GRCGYC 1 cut(s) 1237
Hin6I GCGC 1 cut(s) 1237
HinP1I GCGC 1 cut(s) 1237
HincII GTYRAC 2 cut(s) 216, 850
HindII GTYRAC 2 cut(s) 216, 850
HinfI GANTC 6 cut(s) 542, 588, 641, 1119, 1993, 2056
HpaII CCGG 3 cut(s) 492, 710, 1548
HphI GGTGA 9 cut(s) 58, 131, 293, 987, 1337, 1349, 1462, 1537, 2255
Hpy166II GTNNAC 8 cut(s) 216, 446, 498, 587, 685, 850, 1360, 1534
Hpy188I TCNGA 7 cut(s) 277, 1564, 1630, 1744, 2019, 2182, 2295
Hpy188III TCNNGA 5 cut(s) 173, 1510, 1576, 1688, 2003
Hpy8I GTNNAC 8 cut(s) 216, 446, 498, 587, 685, 850, 1360, 1534
Hpy99I CGWCG 1 cut(s) 1909
HpyAV CCTTC 8 cut(s) 384, 434, 464, 683, 1167, 1294, 1334, 2240
HpyCH4III ACNGT 6 cut(s) 233, 502, 604, 815, 877, 1357
HpyCH4IV ACGT 4 cut(s) 212, 1482, 1692, 1759
HpyF10VI GCNNNNNNNGC 7 cut(s) 89, 159, 295, 367, 623, 1894, 2060
HpyF3I CTNAG 5 cut(s) 276, 581, 1563, 2181, 2190
HpySE526I ACGT 4 cut(s) 212, 1482, 1692, 1759
Hsp92I GRCGYC 1 cut(s) 1237
HspAI GCGC 1 cut(s) 1237
KasI GGCGCC 1 cut(s) 1236
Ksp22I TGATCA 2 cut(s) 85, 1432
Kzo9I GATC 5 cut(s) 85, 1045, 1432, 1589, 1774
LguI GCTCTTC 1 cut(s) 1284
LmnI GCTCC 4 cut(s) 364, 648, 1751, 1894
LweI GCATC 5 cut(s) 12, 604, 613, 1634, 1996
MaeI CTAG 8 cut(s) 311, 1241, 1529, 1796, 1889, 1929, 1952, 2256
MaeII ACGT 4 cut(s) 212, 1482, 1692, 1759
MaeIII GTNAC 9 cut(s) 64, 476, 502, 548, 881, 905, 1022, 1450, 2304
MalI GATC 5 cut(s) 87, 1047, 1434, 1591, 1776
MboI GATC 5 cut(s) 85, 1045, 1432, 1589, 1774
MboII GAAGA 6 cut(s) 817, 1301, 1630, 1952, 1962, 2230
MfeI CAATTG 1 cut(s) 728
MflI RGATCY 2 cut(s) 1045, 1589
MhlI GDGCHC 3 cut(s) 568, 1420, 1645
MlsI TGGCCA 1 cut(s) 254
MluNI TGGCCA 1 cut(s) 254
Mly113I GGCGCC 1 cut(s) 1237
MlyI GAGTC 2 cut(s) 551, 582
MmeI TCCRAC 2 cut(s) 748, 2106
Mox20I TGGCCA 1 cut(s) 254
Mph1103I ATGCAT 1 cut(s) 826
MroXI GAANNNNTTC 3 cut(s) 640, 1009, 1558
MscI TGGCCA 1 cut(s) 254
MseI TTAA 6 cut(s) 653, 765, 1203, 1220, 1296, 2300
MslI CAYNNNNRTG 3 cut(s) 102, 2161, 2313
Msp20I TGGCCA 1 cut(s) 254
MspA1I CMGCKG 3 cut(s) 62, 83, 2271
MspCI CTTAAG 1 cut(s) 1295
MspI CCGG 3 cut(s) 492, 710, 1548
MspR9I CCNGG 5 cut(s) 493, 710, 903, 953, 1002
MunI CAATTG 1 cut(s) 728
Mva1269I GAATGC 2 cut(s) 674, 1991
MvaI CCWGG 3 cut(s) 903, 953, 1002
MwoI GCNNNNNNNGC 7 cut(s) 89, 159, 295, 367, 623, 1894, 2060
NarI GGCGCC 1 cut(s) 1237
NciI CCSGG 2 cut(s) 493, 710
NcoI CCATGG 3 cut(s) 249, 910, 2156
NdeII GATC 5 cut(s) 85, 1045, 1432, 1589, 1774
NheI GCTAGC 1 cut(s) 1888
NlaIV GGNNCC 4 cut(s) 199, 1238, 1417, 1824
NmuCI GTSAC 5 cut(s) 476, 502, 548, 1450, 2304
NsiI ATGCAT 1 cut(s) 826
NspI RCATGY 2 cut(s) 828, 1571
NspV TTCGAA 1 cut(s) 2216
PaeI GCATGC 1 cut(s) 828
PaqCI CACCTGC 1 cut(s) 1313
PceI AGGCCT 1 cut(s) 2098
PciSI GCTCTTC 1 cut(s) 1284
PctI GAATGC 2 cut(s) 674, 1991
PdmI GAANNNNTTC 3 cut(s) 640, 1009, 1558
PfeI GAWTC 4 cut(s) 641, 1119, 1993, 2056
PfoI TCCNGGA 1 cut(s) 1000
PinAI ACCGGT 1 cut(s) 1547
PleI GAGTC 2 cut(s) 550, 582
PluTI GGCGCC 1 cut(s) 1240
PpsI GAGTC 2 cut(s) 550, 582
PsiI TTATAA 1 cut(s) 1317
Psp6I CCWGG 3 cut(s) 901, 951, 1000
PspEI GGTNACC 2 cut(s) 881, 905
PspGI CCWGG 3 cut(s) 901, 951, 1000
PspN4I GGNNCC 4 cut(s) 199, 1238, 1417, 1824
PspPI GGNCC 3 cut(s) 197, 1822, 1932
PstNI CAGNNNCTG 1 cut(s) 83
PsuI RGATCY 2 cut(s) 1045, 1589
PvuII CAGCTG 2 cut(s) 62, 83
RseI CAYNNNNRTG 3 cut(s) 102, 2161, 2313
SalI GTCGAC 1 cut(s) 848
SapI GCTCTTC 1 cut(s) 1284
SaqAI TTAA 6 cut(s) 653, 765, 1203, 1220, 1296, 2300
Sau3AI GATC 5 cut(s) 85, 1045, 1432, 1589, 1774
Sau96I GGNCC 3 cut(s) 197, 1822, 1932
ScaI AGTACT 3 cut(s) 759, 817, 1199
SchI GAGTC 2 cut(s) 551, 582
ScrFI CCNGG 5 cut(s) 493, 710, 903, 953, 1002
SduI GDGCHC 3 cut(s) 568, 1420, 1645
SfaNI GCATC 5 cut(s) 12, 604, 613, 1634, 1996
SfcI CTRYAG 1 cut(s) 1770
SfoI GGCGCC 1 cut(s) 1238
SfuI TTCGAA 1 cut(s) 2216
SgrAI CRCCGGYG 1 cut(s) 1547
SinI GGWCC 2 cut(s) 197, 1822
SmiMI CAYNNNNRTG 3 cut(s) 102, 2161, 2313
SmlI CTYRAG 2 cut(s) 1295, 1782
SmoI CTYRAG 2 cut(s) 1295, 1782
SpeI ACTAGT 2 cut(s) 1528, 1795
SphI GCATGC 1 cut(s) 828
SseBI AGGCCT 1 cut(s) 2098
SsiI CCGC 7 cut(s) 19, 117, 371, 1215, 1892, 2231, 2271
SspDI GGCGCC 1 cut(s) 1236
SspI AATATT 1 cut(s) 1371
SspMI CTAG 8 cut(s) 311, 1241, 1529, 1796, 1889, 1929, 1952, 2256
StuI AGGCCT 1 cut(s) 2098
StyD4I CCNGG 5 cut(s) 491, 708, 901, 951, 1000
StyI CCWWGG 5 cut(s) 249, 609, 910, 925, 2156
TaaI ACNGT 6 cut(s) 233, 502, 604, 815, 877, 1357
TaiI ACGT 4 cut(s) 215, 1485, 1695, 1762
TaqI TCGA 5 cut(s) 849, 942, 1509, 1687, 2216
TaqII GACCGA 2 cut(s) 867, 1529
TatI WGTACW 4 cut(s) 757, 815, 1197, 1539
TauI GCSGC 2 cut(s) 373, 1218
TfiI GAWTC 4 cut(s) 641, 1119, 1993, 2056
Tru1I TTAA 6 cut(s) 653, 765, 1203, 1220, 1296, 2300
Tru9I TTAA 6 cut(s) 653, 765, 1203, 1220, 1296, 2300
TscAI CASTG 4 cut(s) 20, 505, 607, 899
TseFI GTSAC 5 cut(s) 476, 502, 548, 1450, 2304
Tsp45I GTSAC 5 cut(s) 476, 502, 548, 1450, 2304
TspGWI ACGGA 3 cut(s) 909, 1041, 1365
TspRI CASTG 4 cut(s) 20, 505, 607, 899
Vha464I CTTAAG 1 cut(s) 1295
VpaK11BI GGWCC 2 cut(s) 197, 1822
XapI RAATTY 6 cut(s) 176, 1095, 1329, 1998, 2013, 2289
XceI RCATGY 2 cut(s) 828, 1571
XmiI GTMKAC 3 cut(s) 445, 849, 1533
XmnI GAANNNNTTC 3 cut(s) 640, 1009, 1558
XspI CTAG 8 cut(s) 311, 1241, 1529, 1796, 1889, 1929, 1952, 2256
ZrmI AGTACT 3 cut(s) 759, 817, 1199
Zsp2I ATGCAT 1 cut(s) 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.