Rh6CG387600

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000066
Physical Location & Seq
Reverse (-)
57462818 .. 57466237
3420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG387600.1

Sequence Viewer

Length: 2280 bp
ATGTCTATGAGGACAAAGAAAATGTTTTTGCTGCAATTGGTGCTGGCTGCAATAGAGGTGCTACCAGTATCATCCCAAACAGTGGATATTACAAGAGAGGGTTGCCCAGACAAATGTGGCAATGTTAGCATCCCCTACCCGTTCGGAACAAGAAAGGGTTGTTACTATGATGCGGATTTCCTCATTACTTGTAATAGCACAAGTTATAACCCTCCAAAGCCATTTCTGAGAGAGGGTAACATTGAGGTCACAAACATATCCATTGATGGCAAGTTGAATATCATGCAATACATAGCTCAAGATTGCTACGACTCATCTGGTTTCCATATCAAGAACAATCTTCCATCGCTCAACCTTTCAAAATTCAACGTCTCCAACACCGATAATAAGTTCATTGCAGTTGGATGTGACACTTACGCAGAAATCCGAGGCTCCCAGGGAAAAATCAAGAACCACACAGGTGGCTGCATATCAACATGTGACAGCATTGATTATGATGCCAATGATTCATGTTCCGGTATAGGGTGTTGCCAGACATCAATTGCTAAAGGTGTGAGCTATTTTGATGTGTTTTTGAACAGTTATCGGAATCATACAGATGTTTGGTCTTTCAATCCATGCAGCTTCGCCTTTGTTGTGGAAGAAAGTAAGTTCAAATTCTCCAGAGCTATGCTCATGGATTTAAAGAATGTAACAGTACTTCCTGTTGTGCTTGATTGGTCAATTGGAAATGAGACTTGCAAGCAAGTTGAAGGCAAAATGATAGCTGGCTACGCCGGAGGCAGCGCGTGCCAGGGGAACAGCATATGTGTTGATGTCGAAAATGGGTCTGGATACCGATGCAAGTGCAAGGATGGTTTTCAAGGGAACCCATACCTCGCGCTCTCTGATGGTTGTCAAGATATTGATGAATGTGAGGATCCAAACCTCAATAACTGTGCACAAAGATGTGTAAATACAGAGGGAAGTCATCACTGTCTTTGCCGCAAAGGGTACCATGGAAATGGTATAGTAGGGGAAGAAGGTTGCATAGCTAACAGAACATTAGTTGTTCAGATCACTGTTGGAATTGCTGTCGGCCTTATATCGTTGCTAATGTGTAGCTCTTGGTTATATCTGGGATACAGAAGATGGAAACTCATGAAGCTAAAGGAGAGATTCTTCAGACAAAATGGAGGATTAATGTTACAACAACAACTTTCCGAACGACAAGGTGGTGCCACTAACCAAAATGCAAAAATCTTTACTGCAGAAGAACTTGAGAAGGCCACCAACAACTACCATGAGACTAGAATAGTTGGCAAAGGAGGCTACGGTACAGTGTACAGAGGAATTTTATCAGATGGAAGAGTAGTTGCAATCAAGAAATCCAAAATAGTTGATCAAAGCCAAATAGAGCAATTCATTAATGAAGTACTGGTGCTTTCCCGAATCAATCACAGGAATGTGGTGAAGCTTTTGGGTTGTTGTTTCGAGACAGAAGTCCCTCGACTAGTTTATGAGTTTGTCACCAATGGAACGCTATTCGACTACATCCACAACAAAAGCAAGGCATCCAACTTTGCTTGGGAAACTCGTTTAAGGATAGCTGCAGAAGCTGCTGGAGTGCTATCATACTTGCACTCAGCTGCTTCTGTCCCTATTATCCATAGAGATGTCAAGTCCACAAATATACTTTTAGATGAAACCCTTACAGCAAAAGTGTCTGATTTTGGTGCCTCCAGATTGGTACCATTGGATCAAACTGAGTTATCGACAATGGTACAAGGCACGCTCGGGTACTTAGACCCTGAATACTTTCAAACAAGCCAGTTGACTGACAAGAGTGATGTCTATAGCTTTGGGGTTGTCCTGGTGGAGCTACTAACAGGAAAGAAAGCGCTTTCGTTCGACAGGCAAGAGGAGGATAGAAATCTAGCAATGTATTTTCTTTCCGCACTGAAAGAAAACCGCTTGGTACAAGTGGTTGATGAGTGTGTTCGGAATGGGGCAAATGAGGAGCAGCTTAAGGAAGTTTCTAACCTTGCCAAGAGGTGTTTGAAAGTGAAAGGAGAAGAAAGACCAACCATGATGGAAGTAGCAAGCGAGCTTGAGGGACTAAGAAGGATGGCAATGCATCATCCCTGGGTTAATAATGATGAATCCAATGCACAGGAGAATGAAAGCTTGCTTGGTGAAATATCACTGGAAACGTTTAGTCATGGAGGTGGTGGCGATACGAGTTCTGGGTATGATACAATGAGGAACCACATCATATTACCAGTAAATGATGGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase WAK

Protein Analysis

759

Amino Acids

84.21

Weight (kDa)

5.85

Isoelectric Point (pI)

33.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 90 7.9e-11 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 301 - 335 1.9e-07 Calcium-binding EGF domain
Pkinase PF00069 427 - 694 1.3e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 430 - 697 2.8e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 207
Acc65I GGTACC 2 cut(s) 993, 1729
AccB1I GGYRCC 4 cut(s) 993, 1217, 1715, 1729
AccB7I CCANNNNNTGG 1 cut(s) 82
AccII CGCG 2 cut(s) 788, 881
AciI CCGC 4 cut(s) 173, 985, 1935, 1951
AclI AACGTT 1 cut(s) 2192
AclWI GGATC 3 cut(s) 914, 927, 1746
AcsI RAATTY 3 cut(s) 362, 656, 1332
AcuI CTGAAG 1 cut(s) 1147
AfaI GTAC 9 cut(s) 699, 995, 1318, 1325, 1416, 1731, 1764, 1781, 1959
AfeI AGCGCT 1 cut(s) 1881
AfiI CCNNNNNNNGG 2 cut(s) 82, 522
AflII CTTAAG 1 cut(s) 2006
AflIII ACRYGT 1 cut(s) 476
AhlI ACTAGT 1 cut(s) 1492
AjnI CCWGG 4 cut(s) 435, 792, 1851, 2123
AjuI GAANNNNNNNTTGG 2 cut(s) 2154, 2186
AleI CACNNNNGTG 1 cut(s) 459
Alw21I GWGCWC 1 cut(s) 943
Alw26I GTCTC 4 cut(s) 376, 728, 1280, 1469
Alw44I GTGCAC 1 cut(s) 939
AlwI GGATC 3 cut(s) 914, 927, 1746
AlwNI CAGNNNCTG 1 cut(s) 1598
Ama87I CYCGRG 1 cut(s) 1775
Aor51HI AGCGCT 1 cut(s) 1881
AoxI GGCC 2 cut(s) 1078, 1266
ApaLI GTGCAC 1 cut(s) 939
ApeKI GCWGC 9 cut(s) 31, 47, 465, 621, 783, 1589, 1598, 1628, 2002
ApoI RAATTY 3 cut(s) 362, 656, 1332
AseI ATTAAT 2 cut(s) 1181, 1407
Asp700I GAANNNNTTC 1 cut(s) 1797
Asp718I GGTACC 2 cut(s) 993, 1729
AspLEI GCGC 3 cut(s) 788, 883, 1882
AsuHPI GGTGA 3 cut(s) 1462, 1501, 2186
AvaI CYCGRG 1 cut(s) 1775
BaeGI GKGCMC 1 cut(s) 943
BamHI GGATCC 1 cut(s) 919
BanI GGYRCC 4 cut(s) 993, 1217, 1715, 1729
BarI GAAGNNNNNNTAC 2 cut(s) 684, 716
Bbv12I GWGCWC 1 cut(s) 943
BbvI GCAGC 9 cut(s) 18, 34, 452, 633, 795, 1576, 1585, 1615, 2014
BccI CCATC 9 cut(s) 260, 352, 848, 884, 1125, 1337, 2065, 2101, 2264
BcgI CGANNNNNNTGC 2 cut(s) 828, 862
BciT130I CCWGG 4 cut(s) 437, 794, 1853, 2125
BciVI GTATCC 2 cut(s) 827, 1115
BclI TGATCA 1 cut(s) 1381
BcoDI GTCTC 4 cut(s) 376, 728, 1280, 1469
BcuI ACTAGT 1 cut(s) 1492
BfaI CTAG 3 cut(s) 1290, 1493, 1916
BfmI CTRYAG 3 cut(s) 1248, 1590, 1834
BfoI RGCGCY 1 cut(s) 1883
BfrI CTTAAG 1 cut(s) 2006
BfuI GTATCC 2 cut(s) 827, 1115
BmcAI AGTACT 2 cut(s) 699, 1416
Bme1390I CCNGG 4 cut(s) 437, 794, 1853, 2125
BmeT110I CYCGRG 1 cut(s) 1775
BmiI GGNNCC 8 cut(s) 433, 869, 921, 995, 1219, 1717, 1731, 2246
BmrFI CCNGG 4 cut(s) 437, 794, 1853, 2125
BmsI GCATC 6 cut(s) 138, 160, 487, 830, 1562, 2125
BoxI GACNNNNGTC 1 cut(s) 1481
BplI GAGNNNNNCTC 2 cut(s) 657, 689
BpmI CTGGAG 3 cut(s) 646, 1623, 1705
BpuEI CTTGAG 3 cut(s) 282, 1280, 2111
BsaBI GATNNNNATC 1 cut(s) 1911
BsaJI CCNNGG 7 cut(s) 427, 435, 436, 793, 997, 2123, 2124
BsaWI WCCGGW 1 cut(s) 515
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 522
Bse1I ACTGG 5 cut(s) 65, 1422, 1810, 2190, 2261
Bse3DI GCAATG 4 cut(s) 127, 393, 1926, 2118
Bse8I GATNNNNATC 1 cut(s) 1911
BseBI CCWGG 4 cut(s) 437, 794, 1853, 2125
BseDI CCNNGG 7 cut(s) 427, 435, 436, 793, 997, 2123, 2124
BseGI GGATG 8 cut(s) 71, 129, 410, 859, 1533, 1553, 2112, 2119
BseJI GATNNNNATC 1 cut(s) 1911
BseLI CCNNNNNNNGG 2 cut(s) 82, 522
BseMI GCAATG 4 cut(s) 127, 393, 1926, 2118
BseMII CTCAG 3 cut(s) 218, 1638, 1737
BseNI ACTGG 5 cut(s) 65, 1422, 1810, 2190, 2261
BseRI GAGGAG 2 cut(s) 1916, 2012
BseSI GKGCMC 1 cut(s) 943
BseXI GCAGC 9 cut(s) 18, 34, 452, 633, 795, 1576, 1585, 1615, 2014
Bsh1236I CGCG 2 cut(s) 788, 881
BshFI GGCC 2 cut(s) 1080, 1268
BshNI GGYRCC 4 cut(s) 993, 1217, 1715, 1729
BsiHKAI GWGCWC 1 cut(s) 943
BsiHKCI CYCGRG 1 cut(s) 1775
BsiSI CCGG 2 cut(s) 516, 777
BslFI GGGAC 3 cut(s) 1469, 1622, 2109
BslI CCNNNNNNNGG 2 cut(s) 82, 522
BsmAI GTCTC 4 cut(s) 376, 728, 1280, 1469
BsmBI CGTCTC 1 cut(s) 376
BsmFI GGGAC 3 cut(s) 1469, 1622, 2109
BsnI GGCC 2 cut(s) 1080, 1268
BsoBI CYCGRG 1 cut(s) 1775
Bsp1286I GDGCHC 1 cut(s) 943
Bsp1407I TGTACA 1 cut(s) 1323
Bsp143I GATC 4 cut(s) 919, 1056, 1381, 1738
Bsp19I CCATGG 1 cut(s) 997
BspACI CCGC 4 cut(s) 173, 985, 1935, 1951
BspANI GGCC 2 cut(s) 1080, 1268
BspCNI CTCAG 3 cut(s) 219, 1637, 1738
BspFNI CGCG 2 cut(s) 788, 881
BspHI TCATGA 1 cut(s) 1140
BspLI GGNNCC 8 cut(s) 433, 869, 921, 995, 1219, 1717, 1731, 2246
BspMAI CTGCAG 2 cut(s) 1252, 1594
BspPI GGATC 3 cut(s) 914, 927, 1746
BspT107I GGYRCC 4 cut(s) 993, 1217, 1715, 1729
BspTI CTTAAG 1 cut(s) 2006
BsrDI GCAATG 4 cut(s) 127, 393, 1926, 2118
BsrGI TGTACA 1 cut(s) 1323
BsrI ACTGG 5 cut(s) 65, 1422, 1810, 2190, 2261
BssECI CCNNGG 7 cut(s) 427, 435, 436, 793, 997, 2123, 2124
BssMI GATC 4 cut(s) 919, 1056, 1381, 1738
BssT1I CCWWGG 1 cut(s) 997
Bst2UI CCWGG 4 cut(s) 437, 794, 1853, 2125
Bst4CI ACNGT 8 cut(s) 82, 581, 697, 938, 977, 1063, 1316, 1321
Bst6I CTCTTC 1 cut(s) 1342
BstAFI CTTAAG 1 cut(s) 2006
BstAPI GCANNNNNTGC 3 cut(s) 40, 789, 1598
BstAUI TGTACA 1 cut(s) 1323
BstC8I GCNNGC 8 cut(s) 45, 743, 769, 790, 1772, 2083, 2087, 2168
BstDEI CTNAG 5 cut(s) 227, 1624, 1746, 1783, 2099
BstDSI CCRYGG 1 cut(s) 997
BstF5I GGATG 8 cut(s) 71, 129, 410, 859, 1533, 1553, 2112, 2119
BstFNI CGCG 2 cut(s) 788, 881
BstH2I RGCGCY 1 cut(s) 1883
BstHHI GCGC 3 cut(s) 788, 883, 1882
BstKTI GATC 4 cut(s) 922, 1059, 1384, 1741
BstMAI GTCTC 4 cut(s) 376, 728, 1280, 1469
BstMBI GATC 4 cut(s) 919, 1056, 1381, 1738
BstMWI GCNNNNNNNGC 7 cut(s) 40, 126, 773, 789, 1308, 1595, 1598
BstNI CCWGG 4 cut(s) 437, 794, 1853, 2125
BstNSI RCATGY 1 cut(s) 480
BstPAI GACNNNNGTC 1 cut(s) 1481
BstSCI CCNGG 4 cut(s) 435, 792, 1851, 2123
BstSFI CTRYAG 3 cut(s) 1248, 1590, 1834
BstSLI GKGCMC 1 cut(s) 943
BstUI CGCG 2 cut(s) 788, 881
BstV1I GCAGC 9 cut(s) 18, 34, 452, 633, 795, 1576, 1585, 1615, 2014
BstX2I RGATCY 1 cut(s) 919
BstXI CCANNNNNNTGG 2 cut(s) 461, 1004
BstYI RGATCY 1 cut(s) 919
BsuI GTATCC 2 cut(s) 827, 1115
BsuRI GGCC 2 cut(s) 1080, 1268
BtgI CCRYGG 1 cut(s) 997
BtgZI GCGATG 1 cut(s) 330
BtsCI GGATG 8 cut(s) 71, 129, 410, 859, 1533, 1553, 2112, 2119
BtsIMutI CAGTG 6 cut(s) 87, 973, 1059, 1326, 1937, 2183
Cac8I GCNNGC 8 cut(s) 45, 743, 769, 790, 1772, 2083, 2087, 2168
CaiI CAGNNNCTG 1 cut(s) 1598
CciI TCATGA 1 cut(s) 1140
CfoI GCGC 3 cut(s) 788, 883, 1882
Csp6I GTAC 9 cut(s) 698, 994, 1317, 1324, 1415, 1730, 1763, 1780, 1958
CviQI GTAC 9 cut(s) 698, 994, 1317, 1324, 1415, 1730, 1763, 1780, 1958
DdeI CTNAG 5 cut(s) 227, 1624, 1746, 1783, 2099
DpnI GATC 4 cut(s) 921, 1058, 1383, 1740
DpnII GATC 4 cut(s) 919, 1056, 1381, 1738
DraI TTTAAA 1 cut(s) 684
Eam1104I CTCTTC 1 cut(s) 1342
EarI CTCTTC 1 cut(s) 1342
Eco130I CCWWGG 1 cut(s) 997
Eco47III AGCGCT 1 cut(s) 1881
Eco57I CTGAAG 1 cut(s) 1147
Eco88I CYCGRG 1 cut(s) 1775
EcoRII CCWGG 4 cut(s) 435, 792, 1851, 2123
EcoT14I CCWWGG 1 cut(s) 997
EcoT22I ATGCAT 1 cut(s) 2118
ErhI CCWWGG 1 cut(s) 997
Esp3I CGTCTC 1 cut(s) 376
FaqI GGGAC 3 cut(s) 1469, 1622, 2109
FauNDI CATATG 1 cut(s) 806
FbaI TGATCA 1 cut(s) 1381
FokI GGATG 8 cut(s) 58, 116, 417, 866, 1520, 1540, 2106, 2119
FspBI CTAG 3 cut(s) 1290, 1493, 1916
GlaI GCGC 3 cut(s) 787, 882, 1881
GsuI CTGGAG 3 cut(s) 646, 1623, 1705
HaeII RGCGCY 1 cut(s) 1883
HaeIII GGCC 2 cut(s) 1080, 1268
HapII CCGG 2 cut(s) 516, 777
HhaI GCGC 3 cut(s) 788, 883, 1882
Hin6I GCGC 3 cut(s) 786, 881, 1880
HinP1I GCGC 3 cut(s) 786, 881, 1880
HincII GTYRAC 1 cut(s) 1815
HindII GTYRAC 1 cut(s) 1815
HindIII AAGCTT 2 cut(s) 1454, 2164
HinfI GANTC 6 cut(s) 311, 506, 589, 1158, 1431, 2141
HpaII CCGG 2 cut(s) 516, 777
HphI GGTGA 3 cut(s) 1462, 1501, 2186
Hpy166II GTNNAC 4 cut(s) 941, 1324, 1665, 1815
Hpy8I GTNNAC 4 cut(s) 941, 1324, 1665, 1815
HpyAV CCTTC 4 cut(s) 746, 1016, 1258, 2097
HpyCH4III ACNGT 8 cut(s) 82, 581, 697, 938, 977, 1063, 1316, 1321
HpyCH4IV ACGT 2 cut(s) 369, 2192
HpyF10VI GCNNNNNNNGC 7 cut(s) 40, 126, 773, 789, 1308, 1595, 1598
HpyF3I CTNAG 5 cut(s) 227, 1624, 1746, 1783, 2099
HpySE526I ACGT 2 cut(s) 369, 2192
HspAI GCGC 3 cut(s) 786, 881, 1880
KpnI GGTACC 2 cut(s) 997, 1733
Ksp22I TGATCA 1 cut(s) 1381
Kzo9I GATC 4 cut(s) 919, 1056, 1381, 1738
LmnI GCTCC 3 cut(s) 437, 1858, 1999
Lsp1109I GCAGC 9 cut(s) 18, 34, 452, 633, 795, 1576, 1585, 1615, 2014
LweI GCATC 6 cut(s) 138, 160, 487, 830, 1562, 2125
MaeI CTAG 3 cut(s) 1290, 1493, 1916
MaeII ACGT 2 cut(s) 369, 2192
MaeIII GTNAC 8 cut(s) 161, 236, 247, 407, 479, 691, 1185, 1507
MalI GATC 4 cut(s) 921, 1058, 1383, 1740
MboI GATC 4 cut(s) 919, 1056, 1381, 1738
MboII GAAGA 8 cut(s) 332, 653, 1031, 1140, 1153, 1265, 1359, 2066
MfeI CAATTG 3 cut(s) 35, 540, 723
MflI RGATCY 1 cut(s) 919
MhlI GDGCHC 1 cut(s) 943
MluCI AATT 8 cut(s) 35, 362, 540, 656, 723, 1068, 1332, 1400
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 4 cut(s) 382, 399, 1045, 1581
Mph1103I ATGCAT 1 cut(s) 2118
MroXI GAANNNNTTC 1 cut(s) 1797
MseI TTAA 6 cut(s) 683, 1181, 1407, 1580, 2007, 2130
MslI CAYNNNNRTG 7 cut(s) 459, 597, 946, 1002, 1443, 1653, 2205
MspA1I CMGCKG 1 cut(s) 1628
MspCI CTTAAG 1 cut(s) 2006
MspI CCGG 2 cut(s) 516, 777
MspR9I CCNGG 4 cut(s) 437, 794, 1853, 2125
MunI CAATTG 3 cut(s) 35, 540, 723
MvaI CCWGG 4 cut(s) 437, 794, 1853, 2125
MvnI CGCG 2 cut(s) 788, 881
MwoI GCNNNNNNNGC 7 cut(s) 40, 126, 773, 789, 1308, 1595, 1598
NcoI CCATGG 1 cut(s) 997
NdeI CATATG 1 cut(s) 806
NdeII GATC 4 cut(s) 919, 1056, 1381, 1738
NlaIV GGNNCC 8 cut(s) 433, 869, 921, 995, 1219, 1717, 1731, 2246
NmuCI GTSAC 4 cut(s) 247, 407, 479, 1507
NsiI ATGCAT 1 cut(s) 2118
NspI RCATGY 1 cut(s) 480
OliI CACNNNNGTG 1 cut(s) 459
PagI TCATGA 1 cut(s) 1140
PasI CCCWGGG 2 cut(s) 436, 2124
PciI ACATGT 1 cut(s) 476
PdmI GAANNNNTTC 1 cut(s) 1797
PfeI GAWTC 5 cut(s) 506, 589, 1158, 1431, 2141
PflMI CCANNNNNTGG 1 cut(s) 82
PleI GAGTC 1 cut(s) 305
PpsI GAGTC 1 cut(s) 305
PscI ACATGT 1 cut(s) 476
PshAI GACNNNNGTC 1 cut(s) 1481
PshBI ATTAAT 2 cut(s) 1181, 1407
PsiI TTATAA 1 cut(s) 207
Psp1406I AACGTT 1 cut(s) 2192
Psp6I CCWGG 4 cut(s) 435, 792, 1851, 2123
PspGI CCWGG 4 cut(s) 435, 792, 1851, 2123
PspN4I GGNNCC 8 cut(s) 433, 869, 921, 995, 1219, 1717, 1731, 2246
PstI CTGCAG 2 cut(s) 1252, 1594
PstNI CAGNNNCTG 1 cut(s) 1598
PsuI RGATCY 1 cut(s) 919
PvuII CAGCTG 1 cut(s) 1628
RsaI GTAC 9 cut(s) 699, 995, 1318, 1325, 1416, 1731, 1764, 1781, 1959
RsaNI GTAC 9 cut(s) 698, 994, 1317, 1324, 1415, 1730, 1763, 1780, 1958
RseI CAYNNNNRTG 7 cut(s) 459, 597, 946, 1002, 1443, 1653, 2205
SaqAI TTAA 6 cut(s) 683, 1181, 1407, 1580, 2007, 2130
Sau3AI GATC 4 cut(s) 919, 1056, 1381, 1738
ScaI AGTACT 2 cut(s) 699, 1416
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 4 cut(s) 437, 794, 1853, 2125
SduI GDGCHC 1 cut(s) 943
SfaNI GCATC 6 cut(s) 138, 160, 487, 830, 1562, 2125
SfcI CTRYAG 3 cut(s) 1248, 1590, 1834
SmiMI CAYNNNNRTG 7 cut(s) 459, 597, 946, 1002, 1443, 1653, 2205
SmlI CTYRAG 4 cut(s) 297, 1259, 2006, 2090
SmoI CTYRAG 4 cut(s) 297, 1259, 2006, 2090
SpeI ACTAGT 1 cut(s) 1492
Sse9I AATT 8 cut(s) 35, 362, 540, 656, 723, 1068, 1332, 1400
SsiI CCGC 4 cut(s) 173, 985, 1935, 1951
SspMI CTAG 3 cut(s) 1290, 1493, 1916
StyD4I CCNGG 4 cut(s) 435, 792, 1851, 2123
StyI CCWWGG 1 cut(s) 997
TaaI ACNGT 8 cut(s) 82, 581, 697, 938, 977, 1063, 1316, 1321
TaiI ACGT 2 cut(s) 372, 2195
TaqI TCGA 6 cut(s) 819, 1473, 1489, 1527, 1754, 1890
TasI AATT 8 cut(s) 35, 362, 540, 656, 723, 1068, 1332, 1400
TatI WGTACW 3 cut(s) 697, 1323, 1414
TauI GCSGC 1 cut(s) 987
TfiI GAWTC 5 cut(s) 506, 589, 1158, 1431, 2141
Tru1I TTAA 6 cut(s) 683, 1181, 1407, 1580, 2007, 2130
Tru9I TTAA 6 cut(s) 683, 1181, 1407, 1580, 2007, 2130
TscAI CASTG 6 cut(s) 87, 980, 1066, 1326, 1944, 2190
TseFI GTSAC 4 cut(s) 247, 407, 479, 1507
TseI GCWGC 9 cut(s) 31, 47, 465, 621, 783, 1589, 1598, 1628, 2002
Tsp45I GTSAC 4 cut(s) 247, 407, 479, 1507
TspDTI ATGAA 9 cut(s) 382, 498, 924, 1157, 1393, 1425, 1698, 2154, 2175
TspRI CASTG 6 cut(s) 87, 980, 1066, 1326, 1944, 2190
Van91I CCANNNNNTGG 1 cut(s) 82
Vha464I CTTAAG 1 cut(s) 2006
VneI GTGCAC 1 cut(s) 939
VspI ATTAAT 2 cut(s) 1181, 1407
XapI RAATTY 3 cut(s) 362, 656, 1332
XceI RCATGY 1 cut(s) 480
XmnI GAANNNNTTC 1 cut(s) 1797
XspI CTAG 3 cut(s) 1290, 1493, 1916
ZrmI AGTACT 2 cut(s) 699, 1416
Zsp2I ATGCAT 1 cut(s) 2118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.