Wall-associated receptor
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 780 |
| Acc36I | ACCTGC | 2 cut(s) | 171, 780 |
| AccI | GTMKAC | 2 cut(s) | 65, 1076 |
| AciI | CCGC | 3 cut(s) | 47, 114, 546 |
| AcsI | RAATTY | 1 cut(s) | 173 |
| AfaI | GTAC | 2 cut(s) | 55, 581 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 519, 808, 809, 947 |
| AgsI | TTSAA | 1 cut(s) | 906 |
| AjiI | CACGTC | 1 cut(s) | 327 |
| AjnI | CCWGG | 4 cut(s) | 312, 841, 868, 946 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 374, 406 |
| AleI | CACNNNNGTG | 1 cut(s) | 870 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 494, 526 |
| AluBI | AGCT | 9 cut(s) | 77, 94, 293, 333, 621, 679, 697, 752, 1006 |
| AluI | AGCT | 9 cut(s) | 77, 94, 293, 333, 621, 679, 697, 752, 1006 |
| Alw26I | GTCTC | 1 cut(s) | 239 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 80 |
| AoxI | GGCC | 1 cut(s) | 643 |
| ApeKI | GCWGC | 5 cut(s) | 74, 99, 293, 330, 618 |
| ApoI | RAATTY | 1 cut(s) | 173 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 696, 767 |
| AspLEI | GCGC | 1 cut(s) | 518 |
| AsuC2I | CCSGG | 1 cut(s) | 460 |
| AsuHPI | GGTGA | 2 cut(s) | 198, 278 |
| BanII | GRGCYC | 1 cut(s) | 287 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 143, 175, 1063, 1095 |
| BbvI | GCAGC | 5 cut(s) | 86, 86, 280, 342, 630 |
| BccI | CCATC | 1 cut(s) | 217 |
| BceAI | ACGGC | 3 cut(s) | 383, 795, 1006 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 334, 368 |
| BciT130I | CCWGG | 4 cut(s) | 314, 843, 870, 948 |
| BciVI | GTATCC | 1 cut(s) | 971 |
| BcnI | CCSGG | 1 cut(s) | 460 |
| BcoDI | GTCTC | 1 cut(s) | 239 |
| BfaI | CTAG | 1 cut(s) | 51 |
| BfmI | CTRYAG | 3 cut(s) | 294, 616, 1052 |
| BfoI | RGCGCY | 1 cut(s) | 519 |
| BfuAI | ACCTGC | 2 cut(s) | 171, 780 |
| BfuI | GTATCC | 1 cut(s) | 971 |
| BisI | GCNGC | 7 cut(s) | 48, 75, 100, 115, 294, 331, 619 |
| BlsI | GCNGC | 7 cut(s) | 49, 76, 101, 116, 295, 332, 620 |
| BmcAI | AGTACT | 1 cut(s) | 55 |
| Bme1390I | CCNGG | 5 cut(s) | 314, 460, 843, 870, 948 |
| BmgBI | CACGTC | 1 cut(s) | 327 |
| BmiI | GGNNCC | 1 cut(s) | 286 |
| BmrFI | CCNGG | 5 cut(s) | 314, 460, 843, 870, 948 |
| BpmI | CTGGAG | 2 cut(s) | 358, 930 |
| BpuEI | CTTGAG | 1 cut(s) | 69 |
| BpuMI | CCSGG | 1 cut(s) | 460 |
| BsaJI | CCNNGG | 4 cut(s) | 312, 313, 436, 868 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 61, 91, 356, 386, 494, 524, 928, 958 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 519, 808, 809, 947 |
| Bse118I | RCCGGY | 1 cut(s) | 831 |
| Bse1I | ACTGG | 3 cut(s) | 209, 375, 646 |
| BseBI | CCWGG | 4 cut(s) | 314, 843, 870, 948 |
| BseDI | CCNNGG | 4 cut(s) | 312, 313, 436, 868 |
| BseGI | GGATG | 1 cut(s) | 537 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 519, 808, 809, 947 |
| BseMII | CTCAG | 1 cut(s) | 117 |
| BseNI | ACTGG | 3 cut(s) | 209, 375, 646 |
| BseRI | GAGGAG | 2 cut(s) | 453, 456 |
| BseXI | GCAGC | 5 cut(s) | 86, 86, 280, 342, 630 |
| BseYI | CCCAGC | 1 cut(s) | 518 |
| BsgI | GTGCAG | 2 cut(s) | 349, 916 |
| BshFI | GGCC | 1 cut(s) | 645 |
| BsiSI | CCGG | 3 cut(s) | 459, 513, 832 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 519, 808, 809, 947 |
| BsmAI | GTCTC | 1 cut(s) | 239 |
| BsnI | GGCC | 1 cut(s) | 645 |
| Bsp1286I | GDGCHC | 1 cut(s) | 287 |
| Bsp143I | GATC | 2 cut(s) | 369, 1041 |
| BspACI | CCGC | 3 cut(s) | 47, 114, 546 |
| BspANI | GGCC | 1 cut(s) | 645 |
| BspCNI | CTCAG | 1 cut(s) | 116 |
| BspLI | GGNNCC | 1 cut(s) | 286 |
| BspMAI | CTGCAG | 3 cut(s) | 298, 620, 1056 |
| BspMI | ACCTGC | 2 cut(s) | 171, 780 |
| BsrFI | RCCGGY | 1 cut(s) | 831 |
| BsrI | ACTGG | 3 cut(s) | 209, 375, 646 |
| BssAI | RCCGGY | 1 cut(s) | 831 |
| BssECI | CCNNGG | 4 cut(s) | 312, 313, 436, 868 |
| BssMI | GATC | 2 cut(s) | 369, 1041 |
| BssNAI | GTATAC | 1 cut(s) | 1077 |
| Bst1107I | GTATAC | 1 cut(s) | 1077 |
| Bst2UI | CCWGG | 4 cut(s) | 314, 843, 870, 948 |
| Bst4CI | ACNGT | 2 cut(s) | 266, 927 |
| Bst6I | CTCTTC | 2 cut(s) | 363, 441 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 80 |
| BstC8I | GCNNGC | 2 cut(s) | 353, 839 |
| BstDEI | CTNAG | 1 cut(s) | 103 |
| BstF5I | GGATG | 1 cut(s) | 537 |
| BstH2I | RGCGCY | 1 cut(s) | 519 |
| BstHHI | GCGC | 1 cut(s) | 518 |
| BstKTI | GATC | 2 cut(s) | 372, 1044 |
| BstMAI | GTCTC | 1 cut(s) | 239 |
| BstMBI | GATC | 2 cut(s) | 369, 1041 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 56, 80 |
| BstNI | CCWGG | 4 cut(s) | 314, 843, 870, 948 |
| BstSCI | CCNGG | 5 cut(s) | 312, 458, 841, 868, 946 |
| BstSFI | CTRYAG | 3 cut(s) | 294, 616, 1052 |
| BstV1I | GCAGC | 5 cut(s) | 86, 86, 280, 342, 630 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 872 |
| BstZ17I | GTATAC | 1 cut(s) | 1077 |
| BsuI | GTATCC | 1 cut(s) | 971 |
| BsuRI | GGCC | 1 cut(s) | 645 |
| BtrI | CACGTC | 1 cut(s) | 327 |
| BtsCI | GGATG | 1 cut(s) | 537 |
| BtsIMutI | CAGTG | 2 cut(s) | 216, 687 |
| BveI | ACCTGC | 2 cut(s) | 171, 780 |
| Cac8I | GCNNGC | 2 cut(s) | 353, 839 |
| CaiI | CAGNNNCTG | 1 cut(s) | 80 |
| CfoI | GCGC | 1 cut(s) | 518 |
| Cfr10I | RCCGGY | 1 cut(s) | 831 |
| CseI | GACGC | 1 cut(s) | 784 |
| Csp6I | GTAC | 2 cut(s) | 54, 580 |
| CviAII | CATG | 3 cut(s) | 145, 966, 994 |
| CviQI | GTAC | 2 cut(s) | 54, 580 |
| DdeI | CTNAG | 1 cut(s) | 103 |
| DpnI | GATC | 2 cut(s) | 371, 1043 |
| DpnII | GATC | 2 cut(s) | 369, 1041 |
| DraI | TTTAAA | 1 cut(s) | 673 |
| Eam1104I | CTCTTC | 2 cut(s) | 363, 441 |
| EarI | CTCTTC | 2 cut(s) | 363, 441 |
| Eco24I | GRGCYC | 1 cut(s) | 287 |
| EcoRI | GAATTC | 1 cut(s) | 173 |
| EcoRII | CCWGG | 4 cut(s) | 312, 841, 868, 946 |
| EcoT38I | GRGCYC | 1 cut(s) | 287 |
| FaeI | CATG | 3 cut(s) | 148, 969, 997 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 78, 110 |
| FatI | CATG | 3 cut(s) | 144, 965, 993 |
| FauNDI | CATATG | 1 cut(s) | 773 |
| FblI | GTMKAC | 2 cut(s) | 65, 1076 |
| Fnu4HI | GCNGC | 7 cut(s) | 48, 75, 100, 115, 294, 331, 619 |
| FokI | GGATG | 1 cut(s) | 524 |
| FriOI | GRGCYC | 1 cut(s) | 287 |
| Fsp4HI | GCNGC | 7 cut(s) | 48, 75, 100, 115, 294, 331, 619 |
| FspBI | CTAG | 1 cut(s) | 51 |
| GlaI | GCGC | 1 cut(s) | 517 |
| GluI | GCNGC | 7 cut(s) | 48, 75, 100, 115, 294, 331, 619 |
| GsaI | CCCAGC | 1 cut(s) | 522 |
| GsuI | CTGGAG | 2 cut(s) | 358, 930 |
| HaeII | RGCGCY | 1 cut(s) | 519 |
| HaeIII | GGCC | 1 cut(s) | 645 |
| HapII | CCGG | 3 cut(s) | 459, 513, 832 |
| HgaI | GACGC | 1 cut(s) | 784 |
| HhaI | GCGC | 1 cut(s) | 518 |
| Hin1II | CATG | 3 cut(s) | 148, 969, 997 |
| Hin6I | GCGC | 1 cut(s) | 516 |
| HinP1I | GCGC | 1 cut(s) | 516 |
| HincII | GTYRAC | 2 cut(s) | 66, 799 |
| HindII | GTYRAC | 2 cut(s) | 66, 799 |
| HindIII | AAGCTT | 3 cut(s) | 92, 695, 1004 |
| HinfI | GANTC | 3 cut(s) | 12, 232, 1022 |
| HpaII | CCGG | 3 cut(s) | 459, 513, 832 |
| HphI | GGTGA | 2 cut(s) | 198, 278 |
| Hpy166II | GTNNAC | 3 cut(s) | 66, 799, 1077 |
| Hpy188I | TCNGA | 2 cut(s) | 106, 437 |
| Hpy8I | GTNNAC | 3 cut(s) | 66, 799, 1077 |
| Hpy99I | CGWCG | 4 cut(s) | 67, 497, 565, 992 |
| HpyAV | CCTTC | 6 cut(s) | 145, 221, 348, 721, 757, 845 |
| HpyCH4III | ACNGT | 2 cut(s) | 266, 927 |
| HpyCH4IV | ACGT | 5 cut(s) | 62, 326, 411, 492, 560 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 56, 80 |
| HpyF3I | CTNAG | 1 cut(s) | 103 |
| HpySE526I | ACGT | 5 cut(s) | 62, 326, 411, 492, 560 |
| Hsp92II | CATG | 3 cut(s) | 148, 969, 997 |
| HspAI | GCGC | 1 cut(s) | 516 |
| Kzo9I | GATC | 2 cut(s) | 369, 1041 |
| LmnI | GCTCC | 3 cut(s) | 82, 290, 515 |
| Lsp1109I | GCAGC | 5 cut(s) | 86, 86, 280, 342, 630 |
| MaeI | CTAG | 1 cut(s) | 51 |
| MaeII | ACGT | 5 cut(s) | 62, 326, 411, 492, 560 |
| MaeIII | GTNAC | 5 cut(s) | 158, 241, 407, 821, 968 |
| MalI | GATC | 2 cut(s) | 371, 1043 |
| MboI | GATC | 2 cut(s) | 369, 1041 |
| MboII | GAAGA | 5 cut(s) | 160, 182, 333, 350, 458 |
| MfeI | CAATTG | 2 cut(s) | 184, 720 |
| MhlI | GDGCHC | 1 cut(s) | 287 |
| MluCI | AATT | 9 cut(s) | 126, 173, 184, 319, 420, 589, 652, 712, 720 |
| MlyI | GAGTC | 2 cut(s) | 6, 241 |
| MnlI | CCTC | 5 cut(s) | 366, 431, 434, 753, 954 |
| MroXI | GAANNNNTTC | 2 cut(s) | 696, 767 |
| MseI | TTAA | 5 cut(s) | 609, 651, 672, 711, 932 |
| MslI | CAYNNNNRTG | 1 cut(s) | 870 |
| MspI | CCGG | 3 cut(s) | 459, 513, 832 |
| MspR9I | CCNGG | 5 cut(s) | 314, 460, 843, 870, 948 |
| MunI | CAATTG | 2 cut(s) | 184, 720 |
| MvaI | CCWGG | 4 cut(s) | 314, 843, 870, 948 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 56, 80 |
| NciI | CCSGG | 1 cut(s) | 460 |
| NdeI | CATATG | 1 cut(s) | 773 |
| NdeII | GATC | 2 cut(s) | 369, 1041 |
| NlaIII | CATG | 3 cut(s) | 148, 969, 997 |
| NlaIV | GGNNCC | 1 cut(s) | 286 |
| NmuCI | GTSAC | 2 cut(s) | 407, 968 |
| OliI | CACNNNNGTG | 1 cut(s) | 870 |
| PaqCI | CACCTGC | 1 cut(s) | 780 |
| PasI | CCCWGGG | 1 cut(s) | 313 |
| PdmI | GAANNNNTTC | 2 cut(s) | 696, 767 |
| PfeI | GAWTC | 1 cut(s) | 1022 |
| PflFI | GACNNNGTC | 1 cut(s) | 491 |
| PfoI | TCCNGGA | 1 cut(s) | 946 |
| PkrI | GCNGC | 7 cut(s) | 49, 76, 101, 116, 295, 332, 620 |
| PleI | GAGTC | 2 cut(s) | 6, 240 |
| PpsI | GAGTC | 2 cut(s) | 6, 240 |
| Psp6I | CCWGG | 4 cut(s) | 312, 841, 868, 946 |
| PspFI | CCCAGC | 1 cut(s) | 518 |
| PspGI | CCWGG | 4 cut(s) | 312, 841, 868, 946 |
| PspN4I | GGNNCC | 1 cut(s) | 286 |
| PstI | CTGCAG | 3 cut(s) | 298, 620, 1056 |
| PstNI | CAGNNNCTG | 1 cut(s) | 80 |
| PsyI | GACNNNGTC | 1 cut(s) | 491 |
| RsaI | GTAC | 2 cut(s) | 55, 581 |
| RsaNI | GTAC | 2 cut(s) | 54, 580 |
| RseI | CAYNNNNRTG | 1 cut(s) | 870 |
| SalI | GTCGAC | 1 cut(s) | 64 |
| SaqAI | TTAA | 5 cut(s) | 609, 651, 672, 711, 932 |
| SatI | GCNGC | 7 cut(s) | 48, 75, 100, 115, 294, 331, 619 |
| Sau3AI | GATC | 2 cut(s) | 369, 1041 |
| ScaI | AGTACT | 1 cut(s) | 55 |
| SchI | GAGTC | 2 cut(s) | 6, 241 |
| ScrFI | CCNGG | 5 cut(s) | 314, 460, 843, 870, 948 |
| SduI | GDGCHC | 1 cut(s) | 287 |
| SfcI | CTRYAG | 3 cut(s) | 294, 616, 1052 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 870 |
| SmlI | CTYRAG | 1 cut(s) | 84 |
| SmoI | CTYRAG | 1 cut(s) | 84 |
| Sse9I | AATT | 9 cut(s) | 126, 173, 184, 319, 420, 589, 652, 712, 720 |
| SsiI | CCGC | 3 cut(s) | 47, 114, 546 |
| SspI | AATATT | 1 cut(s) | 279 |
| SspMI | CTAG | 1 cut(s) | 51 |
| StyD4I | CCNGG | 5 cut(s) | 312, 458, 841, 868, 946 |
| TaaI | ACNGT | 2 cut(s) | 266, 927 |
| TaiI | ACGT | 5 cut(s) | 65, 329, 414, 495, 563 |
| TaqI | TCGA | 3 cut(s) | 65, 344, 495 |
| TaqII | GACCGA | 1 cut(s) | 553 |
| TasI | AATT | 9 cut(s) | 126, 173, 184, 319, 420, 589, 652, 712, 720 |
| TatI | WGTACW | 1 cut(s) | 53 |
| TauI | GCSGC | 2 cut(s) | 50, 117 |
| TfiI | GAWTC | 1 cut(s) | 1022 |
| Tru1I | TTAA | 5 cut(s) | 609, 651, 672, 711, 932 |
| Tru9I | TTAA | 5 cut(s) | 609, 651, 672, 711, 932 |
| TscAI | CASTG | 2 cut(s) | 216, 694 |
| TseFI | GTSAC | 2 cut(s) | 407, 968 |
| TseI | GCWGC | 5 cut(s) | 74, 99, 293, 330, 618 |
| Tsp45I | GTSAC | 2 cut(s) | 407, 968 |
| TspDTI | ATGAA | 7 cut(s) | 161, 760, 762, 864, 954, 1010, 1053 |
| TspGWI | ACGGA | 3 cut(s) | 277, 460, 936 |
| TspRI | CASTG | 2 cut(s) | 216, 694 |
| Tth111I | GACNNNGTC | 1 cut(s) | 491 |
| XapI | RAATTY | 1 cut(s) | 173 |
| XmiI | GTMKAC | 2 cut(s) | 65, 1076 |
| XmnI | GAANNNNTTC | 2 cut(s) | 696, 767 |
| XspI | CTAG | 1 cut(s) | 51 |
| ZrmI | AGTACT | 1 cut(s) | 55 |