MD10G1248500.v1.1

specification of plant organ axis polarity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000066
Physical Location & Seq
Forward (+)
34164259 .. 34170421
6163 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1248500.v1.1.491

Sequence Viewer

Length: 2679 bp
ATGGCGTTACATAGGAGGATGCATATTTTGCATATCTCTTTAATAATGGTGGTAGTGGTTACAATTTTACTAGGAACTCCAACTCCAACAACAGCTCAATCTCTCCTTCCGCCTCAGGCACTGCCTGGCTGCCCTGACCATTGTGGTAATCTCACAATTCCATACCCATTTGGCATAGGCTCTGGTTGCTACCTCCAAAGCCGGTTTAACATCACTTGTGAAAATTCCACTCAACAGCCAACAACACGTTTGATGACGAGCAATATCGAAATTACCAACATATCTCTTTATGACGGTGAGCTCCAAATACTACAGTTCGCAGCCAATGATTGTTATGATGCTCAAGGCAACTTGACCGCACGGAACAATTCCAGGCTCTGGGTTCCTCCTCCTTACACCATCTCCGAAACCAAAAACATGTTCGTCGCAGTTGGATGCGACACATATGCGTTTTTTACAGGTCACCGGGATGAACAAAAGTACACAACTGGGTGCATGTCCATATGTGACAATCTTGACAACGCTATCGATCAGCGGGATACTTGCTCTGGCGTTGGATGTTGCCAAACTAAAATCCCTAGTGGGCTGAAAAATCAGACTGTGACCTTGAGAAGCTTTAACAATCATACGGATGTGTGGGACTTCAACCCATGCAGCTACGCGTTCCTTGTGCAAGAAGGCGAGTTCAACTTTAACAGCACAACTAAAAACGAGAGCACTTTTGCATGCAAAGCAAATAGCTCCTGCCATAATCGGTCTTCAGGCTACATATGCCGGTGCCTGCCAGGTTACGAAGGGAACCCATACCTCCCGGATGGTTGCCAAGATATTGATGAGTGCAAGGCTTCAGACTCCTGCAGTATTGGAACCTGCATAAATACAGCGGGAAATTATACTTGTGAATGTCCGAAAGGGTACAAAAATGACGATATGGATAAAAAAAGTTGCATCAAGGACAACGGTCGAGCAAAGCTCAGTCTCCTGCTTATAATTTCACTAGGTGCCAGTGGAGGCTTCATGCTTTTACTAAGTGGATCTTCTTGGATATATTGGGGAATGCAGAGAAGAAAGTTCCTCAAACTCAAAGAAAAATACTTTAAAGAAAATGGTGGATTATTGTTACAACAAAAAATCGCTAGTCAAGGAGGCTCTGTGGAGACAACAAAAATTTTCACGGCAGAAGAACTTGAGAAGGCAACAAACAATTACCATGAAAGTAGAATCCTTGGTGAAGGAGGCTATGGAATAGTTTACAAAGGAATATTAGCAGCAGATAACAATAAAGTGGTTGCCATAAAAAAGTCTAAAATTGGTGTCCCAACCCAGAAGGAGCAGTTTGTCAATGAGTTGATTGTTCTTTCTCAAATCAACCATAGAAATGTGGTGAGGTTATTGGGTTGTTGTTTGGAGTCAGAAGTTCCTCTACTAGTTTACGAGTTTGTCACTCACGGCACTCTCTTTGAGCACATTCATGGTAAAAAGAGGAAAGGATCACCATTTTCATTGGAATTACGACTCAAGATAGCAGCTGAAACTGCTGGAGCACTAGCATACTTACACTCCTCCGCTCTCATGCAAATTATACACCGAGATGTGAAGGCGACCAATATACTGCTAGATGAAAATTACACTGCAAAAGTGTCAGACTTTGGAGCTTCACGATTGATTCCTCTTGATCAAGCCCAACTTGCAACTTTAGTGCAAGGAACATTCGGATACTTAGACCCTGAATACTTCCTCACAAATCAACTAACAGATAAAAGTGACGTCTATAGCTTTGGAGTTGTCCTTATTGAGCTACTAACAAGCAAAGTAGCACTTTCTTTTGACAGGCCTGAGGAAGAGAGAAACCTAGCAAGCTTCTTTGTTTGCTTAATGGAGGAGGATCGCTTGAATCAAATTCTCGATGATGCCATAATCAACGAGAGAAACATTGAGACACTAAAAACAATGGCCAATCTCGCAAAAAGATGTGTAAGGTTAAAAGGGGAGGACAGGCCAACAATGAAAGAAGTTGCCATGGAGCTGGAAGGAATGAGAATTACAGAAAAGCATGCATGGGGAAAAGCTGAAATTTTTTCAGAAGAGACTGAGTACTTACTTGGGACACCTAACTCAGATGCTTATCCTGTGGCTTTTAGAGCTGATTGTGGTCCTAGTATTGGTACAGCTAGTGAAAAAATAATTATATCAAACTCAGAGAAAAGTATTTCAAAGAAAAGGGTGGAGACAACAACAATCTTTACAGCAGTACTTAAGAAGGGCACAGACAACTATCATGAGAGCAGAGTCGTCGGCGAAGGAGGCTTCGGAACAGTCTACAAAGGAATACAACCGGATAATGAAGTGGTTGCCATAAAAAAGTCGAAAATCGGAGCTTCAACCCAATGTGAGCAGTTTATCAATGAAGTTATCGTTCTTTCTCAAGTCAACCATAGAAATGTGGTGAAACTATTGGGTTGCTGTTTAGAGACACCGGTGTCATTACTAGTTTACGAGTTCATCACCGGGGCAACTCTTTTTTATCATCTTCATAGCATAAAGAGCAAAGAATCATCGGTTTCATGGGAGTTACGATTGAATATCGCAGCAGAAACTGCCAGAGCACTGGCATACTTATTACTTGCGCTCCTCCACCTCCATGCCACCTCCATGCCAATCATACACCGAGATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase_Tyr

Protein Analysis

893

Amino Acids

98.92

Weight (kDa)

6.37

Isoelectric Point (pI)

37.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 44 - 103 2.7e-12 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 276 - 314 5.8e-07 Calcium-binding EGF domain
Pkinase PF00069 403 - 672 1.1e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 406 - 676 3.7e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 759 - 892 7.6e-18 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 762 - 891 3e-17 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 989
AasI GACNNNNNNGTC 1 cut(s) 2479
AatII GACGTC 1 cut(s) 1770
Acc36I ACCTGC 1 cut(s) 878
AccB1I GGYRCC 2 cut(s) 777, 1001
AccB7I CCANNNNNTGG 1 cut(s) 378
AccBSI CCGCTC 1 cut(s) 1568
AccI GTMKAC 1 cut(s) 2319
AccII CGCG 1 cut(s) 662
AciI CCGC 5 cut(s) 110, 357, 535, 884, 1566
AclWI GGATC 3 cut(s) 1042, 1498, 1893
AcoI YGGCCR 1 cut(s) 1953
AcsI RAATTY 4 cut(s) 223, 1167, 1899, 2073
AcuI CTGAAG 2 cut(s) 744, 831
AcyI GRCGYC 1 cut(s) 1767
AdeI CACNNNGTG 1 cut(s) 1001
AfaI GTAC 5 cut(s) 482, 917, 2096, 2167, 2253
AfiI CCNNNNNNNGG 2 cut(s) 378, 2162
AflII CTTAAG 1 cut(s) 2255
AflIII ACRYGT 3 cut(s) 245, 417, 660
AgeI ACCGGT 1 cut(s) 2476
AgsI TTSAA 6 cut(s) 646, 688, 1894, 2214, 2382, 2581
AhlI ACTAGT 2 cut(s) 1426, 2488
AjnI CCWGG 3 cut(s) 124, 371, 784
AjuI GAANNNNNNNTTGG 2 cut(s) 404, 436
AloI GAACNNNNNNTCC 2 cut(s) 67, 99
Alw21I GWGCWC 5 cut(s) 303, 719, 1467, 1546, 2608
Alw26I GTCTC 6 cut(s) 983, 1151, 1931, 2081, 2222, 2465
AlwI GGATC 3 cut(s) 1042, 1498, 1893
AlwNI CAGNNNCTG 3 cut(s) 121, 378, 2597
AoxI GGCC 3 cut(s) 1832, 1953, 1997
ApeKI GCWGC 6 cut(s) 129, 320, 654, 1268, 1526, 2588
ApoI RAATTY 4 cut(s) 223, 1167, 1899, 2073
AsiGI ACCGGT 1 cut(s) 2476
AspLEI GCGC 1 cut(s) 2629
AspS9I GGNCC 1 cut(s) 2153
AsuC2I CCSGG 3 cut(s) 467, 812, 2509
AsuHPI GGTGA 7 cut(s) 308, 455, 1241, 1396, 1485, 2458, 2497
AvaII GGWCC 1 cut(s) 2153
AxyI CCTNAGG 2 cut(s) 114, 1836
BaeGI GKGCMC 1 cut(s) 2267
BalI TGGCCA 1 cut(s) 1955
BanI GGYRCC 2 cut(s) 777, 1001
BanII GRGCYC 1 cut(s) 303
BarI GAAGNNNNNNTAC 2 cut(s) 1407, 1439
BbsI GAAGAC 1 cut(s) 750
Bbv12I GWGCWC 5 cut(s) 303, 719, 1467, 1546, 2608
BbvI GCAGC 6 cut(s) 116, 332, 666, 1280, 1538, 2600
BccI CCATC 2 cut(s) 407, 809
BceAI ACGGC 2 cut(s) 1191, 1465
BcgI CGANNNNNNTGC 4 cut(s) 428, 462, 2275, 2309
BciT130I CCWGG 3 cut(s) 126, 373, 786
BciVI GTATCC 2 cut(s) 532, 1709
BclI TGATCA 1 cut(s) 1675
BcnI CCSGG 3 cut(s) 467, 812, 2509
BcoDI GTCTC 6 cut(s) 983, 1151, 1931, 2081, 2222, 2465
BcuI ACTAGT 2 cut(s) 1426, 2488
BfmI CTRYAG 3 cut(s) 311, 856, 1771
BfrI CTTAAG 1 cut(s) 2255
BfuAI ACCTGC 1 cut(s) 878
BfuI GTATCC 2 cut(s) 532, 1709
BisI GCNGC 6 cut(s) 130, 321, 655, 1269, 1527, 2589
BlsI GCNGC 6 cut(s) 131, 322, 656, 1270, 1528, 2590
BmcAI AGTACT 2 cut(s) 2096, 2253
Bme1390I CCNGG 6 cut(s) 126, 373, 467, 786, 812, 2509
Bme18I GGWCC 1 cut(s) 2153
BmgT120I GGNCC 1 cut(s) 2153
BmiI GGNNCC 5 cut(s) 384, 779, 800, 868, 1003
BmrFI CCNGG 6 cut(s) 126, 373, 467, 786, 812, 2509
BmrI ACTGGG 1 cut(s) 498
BmsI GCATC 6 cut(s) 9, 328, 425, 957, 1900, 2110
BmuI ACTGGG 1 cut(s) 498
BoxI GACNNNNGTC 1 cut(s) 960
BpiI GAAGAC 1 cut(s) 750
BplI GAGNNNNNCTC 2 cut(s) 957, 989
BpmI CTGGAG 1 cut(s) 1560
BpuEI CTTGAG 5 cut(s) 327, 628, 1208, 1502, 2409
BpuMI CCSGG 3 cut(s) 467, 812, 2509
Bsa29I ATCGAT 1 cut(s) 528
BsaBI GATNNNNATC 1 cut(s) 2124
BsaHI GRCGYC 1 cut(s) 1767
BsaJI CCNNGG 3 cut(s) 1225, 2019, 2508
BsaWI WCCGGW 2 cut(s) 2335, 2476
Bsc4I CCNNNNNNNGG 2 cut(s) 378, 2162
Bse118I RCCGGY 3 cut(s) 201, 774, 2476
Bse1I ACTGG 3 cut(s) 493, 1005, 2613
Bse21I CCTNAGG 2 cut(s) 114, 1836
Bse8I GATNNNNATC 1 cut(s) 2124
BseBI CCWGG 3 cut(s) 126, 373, 786
BseCI ATCGAT 1 cut(s) 528
BseDI CCNNGG 3 cut(s) 1225, 2019, 2508
BseGI GGATG 6 cut(s) 24, 440, 475, 563, 637, 820
BseJI GATNNNNATC 1 cut(s) 2124
BseLI CCNNNNNNNGG 2 cut(s) 378, 2162
BseMII CTCAG 6 cut(s) 128, 988, 1827, 2082, 2130, 2211
BseNI ACTGG 3 cut(s) 493, 1005, 2613
BseRI GAGGAG 4 cut(s) 378, 1552, 1895, 2621
BseSI GKGCMC 1 cut(s) 2267
BseXI GCAGC 6 cut(s) 116, 332, 666, 1280, 1538, 2600
Bsh1236I CGCG 1 cut(s) 662
Bsh1285I CGRYCG 1 cut(s) 964
BshFI GGCC 3 cut(s) 1834, 1955, 1999
BshNI GGYRCC 2 cut(s) 777, 1001
BshTI ACCGGT 1 cut(s) 2476
BshVI ATCGAT 1 cut(s) 528
BsiEI CGRYCG 1 cut(s) 964
BsiHKAI GWGCWC 5 cut(s) 303, 719, 1467, 1546, 2608
BsiSI CCGG 7 cut(s) 202, 466, 775, 812, 2336, 2477, 2508
BslFI GGGAC 3 cut(s) 653, 1301, 2119
BslI CCNNNNNNNGG 2 cut(s) 378, 2162
BsmAI GTCTC 6 cut(s) 983, 1151, 1931, 2081, 2222, 2465
BsmFI GGGAC 3 cut(s) 653, 1301, 2119
BsmI GAATGC 1 cut(s) 1062
BsnI GGCC 3 cut(s) 1834, 1955, 1999
Bsp1286I GDGCHC 6 cut(s) 303, 719, 1467, 1546, 2267, 2608
Bsp143I GATC 5 cut(s) 529, 1034, 1490, 1675, 1885
Bsp19I CCATGG 1 cut(s) 2019
BspACI CCGC 5 cut(s) 110, 357, 535, 884, 1566
BspANI GGCC 3 cut(s) 1834, 1955, 1999
BspCNI CTCAG 6 cut(s) 127, 987, 1828, 2083, 2129, 2210
BspDI ATCGAT 1 cut(s) 528
BspFNI CGCG 1 cut(s) 662
BspHI TCATGA 1 cut(s) 2278
BspLI GGNNCC 5 cut(s) 384, 779, 800, 868, 1003
BspMAI CTGCAG 1 cut(s) 860
BspMI ACCTGC 1 cut(s) 878
BspPI GGATC 3 cut(s) 1042, 1498, 1893
BspT107I GGYRCC 2 cut(s) 777, 1001
BspTI CTTAAG 1 cut(s) 2255
BsrBI CCGCTC 1 cut(s) 1568
BsrFI RCCGGY 3 cut(s) 201, 774, 2476
BsrI ACTGG 3 cut(s) 493, 1005, 2613
BssAI RCCGGY 3 cut(s) 201, 774, 2476
BssECI CCNNGG 3 cut(s) 1225, 2019, 2508
BssMI GATC 5 cut(s) 529, 1034, 1490, 1675, 1885
BssNI GRCGYC 1 cut(s) 1767
BssT1I CCWWGG 2 cut(s) 1225, 2019
Bst2UI CCWGG 3 cut(s) 126, 373, 786
Bst4CI ACNGT 5 cut(s) 296, 315, 601, 962, 2317
Bst6I CTCTTC 2 cut(s) 1836, 2079
BstACI GRCGYC 1 cut(s) 1767
BstAFI CTTAAG 1 cut(s) 2255
BstAPI GCANNNNNTGC 2 cut(s) 28, 2597
BstC8I GCNNGC 4 cut(s) 727, 782, 1858, 2055
BstDEI CTNAG 8 cut(s) 114, 974, 1028, 1720, 1836, 2091, 2116, 2197
BstDSI CCRYGG 1 cut(s) 2019
BstEII GGTNACC 1 cut(s) 461
BstF5I GGATG 6 cut(s) 24, 440, 475, 563, 637, 820
BstFNI CGCG 1 cut(s) 662
BstHHI GCGC 1 cut(s) 2629
BstKTI GATC 5 cut(s) 532, 1037, 1493, 1678, 1888
BstMAI GTCTC 6 cut(s) 983, 1151, 1931, 2081, 2222, 2465
BstMBI GATC 5 cut(s) 529, 1034, 1490, 1675, 1885
BstMCI CGRYCG 1 cut(s) 964
BstNI CCWGG 3 cut(s) 126, 373, 786
BstNSI RCATGY 4 cut(s) 421, 499, 729, 2057
BstPAI GACNNNNGTC 1 cut(s) 960
BstPI GGTNACC 1 cut(s) 461
BstSCI CCNGG 6 cut(s) 124, 371, 465, 784, 810, 2507
BstSFI CTRYAG 3 cut(s) 311, 856, 1771
BstSLI GKGCMC 1 cut(s) 2267
BstUI CGCG 1 cut(s) 662
BstV1I GCAGC 6 cut(s) 116, 332, 666, 1280, 1538, 2600
BstV2I GAAGAC 1 cut(s) 750
BstX2I RGATCY 1 cut(s) 1034
BstXI CCANNNNNNTGG 2 cut(s) 2026, 2608
BstYI RGATCY 1 cut(s) 1034
Bsu15I ATCGAT 1 cut(s) 528
Bsu36I CCTNAGG 2 cut(s) 114, 1836
BsuI GTATCC 2 cut(s) 532, 1709
BsuRI GGCC 3 cut(s) 1834, 1955, 1999
BsuTUI ATCGAT 1 cut(s) 528
BtgI CCRYGG 1 cut(s) 2019
BtsCI GGATG 6 cut(s) 24, 440, 475, 563, 637, 820
BtsI GCAGTG 2 cut(s) 119, 1629
BtsIMutI CAGTG 4 cut(s) 119, 1012, 1629, 2606
BveI ACCTGC 1 cut(s) 878
Cac8I GCNNGC 4 cut(s) 727, 782, 1858, 2055
CaiI CAGNNNCTG 3 cut(s) 121, 378, 2597
CciI TCATGA 1 cut(s) 2278
CfoI GCGC 1 cut(s) 2629
Cfr10I RCCGGY 3 cut(s) 201, 774, 2476
Cfr13I GGNCC 1 cut(s) 2153
ClaI ATCGAT 1 cut(s) 528
Csp6I GTAC 5 cut(s) 481, 916, 2095, 2166, 2252
CspAI ACCGGT 1 cut(s) 2476
CviQI GTAC 5 cut(s) 481, 916, 2095, 2166, 2252
DdeI CTNAG 8 cut(s) 114, 974, 1028, 1720, 1836, 2091, 2116, 2197
DpnI GATC 5 cut(s) 531, 1036, 1492, 1677, 1887
DpnII GATC 5 cut(s) 529, 1034, 1490, 1675, 1885
DraI TTTAAA 1 cut(s) 1099
DraIII CACNNNGTG 1 cut(s) 1001
DrdI GACNNNNNNGTC 1 cut(s) 2479
DseDI GACNNNNNNGTC 1 cut(s) 2479
EaeI YGGCCR 1 cut(s) 1953
Eam1104I CTCTTC 2 cut(s) 1836, 2079
EarI CTCTTC 2 cut(s) 1836, 2079
EciI GGCGGA 1 cut(s) 99
Ecl136II GAGCTC 1 cut(s) 301
Eco130I CCWWGG 2 cut(s) 1225, 2019
Eco147I AGGCCT 1 cut(s) 1834
Eco24I GRGCYC 1 cut(s) 303
Eco47I GGWCC 1 cut(s) 2153
Eco53kI GAGCTC 1 cut(s) 301
Eco57I CTGAAG 2 cut(s) 744, 831
Eco81I CCTNAGG 2 cut(s) 114, 1836
Eco91I GGTNACC 1 cut(s) 461
EcoICRI GAGCTC 1 cut(s) 301
EcoO65I GGTNACC 1 cut(s) 461
EcoRII CCWGG 3 cut(s) 124, 371, 784
EcoT14I CCWWGG 2 cut(s) 1225, 2019
EcoT22I ATGCAT 2 cut(s) 24, 2059
EcoT38I GRGCYC 1 cut(s) 303
ErhI CCWWGG 2 cut(s) 1225, 2019
FalI AAGNNNNNCTT 6 cut(s) 1021, 1053, 1671, 1703, 1803, 1835
FaqI GGGAC 3 cut(s) 653, 1301, 2119
FauI CCCGC 2 cut(s) 528, 877
FauNDI CATATG 3 cut(s) 445, 503, 770
FbaI TGATCA 1 cut(s) 1675
FblI GTMKAC 1 cut(s) 2319
Fnu4HI GCNGC 6 cut(s) 130, 321, 655, 1269, 1527, 2589
FokI GGATG 6 cut(s) 31, 447, 482, 570, 644, 827
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 6 cut(s) 130, 321, 655, 1269, 1527, 2589
GlaI GCGC 1 cut(s) 2628
GluI GCNGC 6 cut(s) 130, 321, 655, 1269, 1527, 2589
GsuI CTGGAG 1 cut(s) 1560
HaeIII GGCC 3 cut(s) 1834, 1955, 1999
HapII CCGG 7 cut(s) 202, 466, 775, 812, 2336, 2477, 2508
HhaI GCGC 1 cut(s) 2629
Hin1I GRCGYC 1 cut(s) 1767
Hin6I GCGC 1 cut(s) 2627
HinP1I GCGC 1 cut(s) 2627
HincII GTYRAC 1 cut(s) 2431
HindII GTYRAC 1 cut(s) 2431
HindIII AAGCTT 2 cut(s) 613, 1858
HinfI GANTC 8 cut(s) 851, 1221, 1409, 1515, 1666, 1894, 2289, 2552
HpaII CCGG 7 cut(s) 202, 466, 775, 812, 2336, 2477, 2508
HphI GGTGA 7 cut(s) 308, 455, 1241, 1396, 1485, 2458, 2497
Hpy166II GTNNAC 6 cut(s) 483, 1252, 1432, 2320, 2431, 2494
Hpy188III TCNNGA 6 cut(s) 515, 1519, 1659, 1673, 1904, 2279
Hpy8I GTNNAC 6 cut(s) 483, 1252, 1432, 2320, 2431, 2494
Hpy99I CGWCG 2 cut(s) 428, 2297
HpyCH4III ACNGT 5 cut(s) 296, 315, 601, 962, 2317
HpyCH4IV ACGT 2 cut(s) 247, 1767
HpyF3I CTNAG 8 cut(s) 114, 974, 1028, 1720, 1836, 2091, 2116, 2197
HpySE526I ACGT 2 cut(s) 247, 1767
Hsp92I GRCGYC 1 cut(s) 1767
HspAI GCGC 1 cut(s) 2627
Ksp22I TGATCA 1 cut(s) 1675
Kzo9I GATC 5 cut(s) 529, 1034, 1490, 1675, 1885
LmnI GCTCC 8 cut(s) 306, 746, 1330, 1541, 1652, 2023, 2375, 2634
Lsp1109I GCAGC 6 cut(s) 116, 332, 666, 1280, 1538, 2600
LweI GCATC 6 cut(s) 9, 328, 425, 957, 1900, 2110
MaeII ACGT 2 cut(s) 247, 1767
MalI GATC 5 cut(s) 531, 1036, 1492, 1677, 1887
MbiI CCGCTC 1 cut(s) 1568
MboI GATC 5 cut(s) 529, 1034, 1490, 1675, 1885
MboII GAAGA 7 cut(s) 750, 1029, 1077, 1193, 1853, 2096, 2522
MflI RGATCY 1 cut(s) 1034
MhlI GDGCHC 6 cut(s) 303, 719, 1467, 1546, 2267, 2608
MlsI TGGCCA 1 cut(s) 1955
MluI ACGCGT 1 cut(s) 660
MluNI TGGCCA 1 cut(s) 1955
MlyI GAGTC 4 cut(s) 845, 1418, 1509, 2298
MmeI TCCRAC 4 cut(s) 104, 110, 412, 535
Mox20I TGGCCA 1 cut(s) 1955
Mph1103I ATGCAT 2 cut(s) 24, 2059
MscI TGGCCA 1 cut(s) 1955
MseI TTAA 8 cut(s) 41, 207, 618, 693, 1098, 1874, 1982, 2256
MslI CAYNNNNRTG 9 cut(s) 468, 630, 1377, 1470, 1590, 2439, 2640, 2651, 2670
Msp20I TGGCCA 1 cut(s) 1955
MspA1I CMGCKG 3 cut(s) 535, 884, 1529
MspCI CTTAAG 1 cut(s) 2255
MspI CCGG 7 cut(s) 202, 466, 775, 812, 2336, 2477, 2508
MspR9I CCNGG 6 cut(s) 126, 373, 467, 786, 812, 2509
Mva1269I GAATGC 1 cut(s) 1062
MvaI CCWGG 3 cut(s) 126, 373, 786
MvnI CGCG 1 cut(s) 662
NciI CCSGG 3 cut(s) 467, 812, 2509
NcoI CCATGG 1 cut(s) 2019
NdeI CATATG 3 cut(s) 445, 503, 770
NdeII GATC 5 cut(s) 529, 1034, 1490, 1675, 1885
NlaIV GGNNCC 5 cut(s) 384, 779, 800, 868, 1003
NmuCI GTSAC 5 cut(s) 461, 506, 601, 1441, 1763
NsiI ATGCAT 2 cut(s) 24, 2059
NspI RCATGY 4 cut(s) 421, 499, 729, 2057
PaeI GCATGC 2 cut(s) 729, 2057
PagI TCATGA 1 cut(s) 2278
PceI AGGCCT 1 cut(s) 1834
PciI ACATGT 1 cut(s) 417
PctI GAATGC 1 cut(s) 1062
PfeI GAWTC 4 cut(s) 1221, 1666, 1894, 2552
PflMI CCANNNNNTGG 1 cut(s) 378
PfoI TCCNGGA 1 cut(s) 810
PinAI ACCGGT 1 cut(s) 2476
PkrI GCNGC 6 cut(s) 131, 322, 656, 1270, 1528, 2590
PleI GAGTC 4 cut(s) 845, 1417, 1509, 2297
PpsI GAGTC 4 cut(s) 845, 1417, 1509, 2297
PscI ACATGT 1 cut(s) 417
PshAI GACNNNNGTC 1 cut(s) 960
PsiI TTATAA 1 cut(s) 989
Psp124BI GAGCTC 1 cut(s) 303
Psp6I CCWGG 3 cut(s) 124, 371, 784
PspEI GGTNACC 1 cut(s) 461
PspGI CCWGG 3 cut(s) 124, 371, 784
PspN4I GGNNCC 5 cut(s) 384, 779, 800, 868, 1003
PspPI GGNCC 1 cut(s) 2153
PstI CTGCAG 1 cut(s) 860
PstNI CAGNNNCTG 3 cut(s) 121, 378, 2597
PsuI RGATCY 1 cut(s) 1034
PvuII CAGCTG 1 cut(s) 1529
RsaI GTAC 5 cut(s) 482, 917, 2096, 2167, 2253
RsaNI GTAC 5 cut(s) 481, 916, 2095, 2166, 2252
RseI CAYNNNNRTG 9 cut(s) 468, 630, 1377, 1470, 1590, 2439, 2640, 2651, 2670
SacI GAGCTC 1 cut(s) 303
SaqAI TTAA 8 cut(s) 41, 207, 618, 693, 1098, 1874, 1982, 2256
SatI GCNGC 6 cut(s) 130, 321, 655, 1269, 1527, 2589
Sau3AI GATC 5 cut(s) 529, 1034, 1490, 1675, 1885
Sau96I GGNCC 1 cut(s) 2153
ScaI AGTACT 2 cut(s) 2096, 2253
SchI GAGTC 4 cut(s) 845, 1418, 1509, 2298
ScrFI CCNGG 6 cut(s) 126, 373, 467, 786, 812, 2509
SduI GDGCHC 6 cut(s) 303, 719, 1467, 1546, 2267, 2608
SfaNI GCATC 6 cut(s) 9, 328, 425, 957, 1900, 2110
SfcI CTRYAG 3 cut(s) 311, 856, 1771
SgrAI CRCCGGYG 1 cut(s) 2476
SinI GGWCC 1 cut(s) 2153
SmiMI CAYNNNNRTG 9 cut(s) 468, 630, 1377, 1470, 1590, 2439, 2640, 2651, 2670
SmlI CTYRAG 6 cut(s) 342, 607, 1187, 1517, 2255, 2424
SmoI CTYRAG 6 cut(s) 342, 607, 1187, 1517, 2255, 2424
SpeI ACTAGT 2 cut(s) 1426, 2488
SphI GCATGC 2 cut(s) 729, 2057
SseBI AGGCCT 1 cut(s) 1834
SsiI CCGC 5 cut(s) 110, 357, 535, 884, 1566
SspI AATATT 1 cut(s) 1263
SstI GAGCTC 1 cut(s) 303
StuI AGGCCT 1 cut(s) 1834
StyD4I CCNGG 6 cut(s) 124, 371, 465, 784, 810, 2507
StyI CCWWGG 2 cut(s) 1225, 2019
TaaI ACNGT 5 cut(s) 296, 315, 601, 962, 2317
TaiI ACGT 2 cut(s) 250, 1770
TaqI TCGA 5 cut(s) 267, 528, 964, 1905, 2366
TaqII GACCGA 1 cut(s) 744
TatI WGTACW 3 cut(s) 480, 2094, 2251
TfiI GAWTC 4 cut(s) 1221, 1666, 1894, 2552
Tru1I TTAA 8 cut(s) 41, 207, 618, 693, 1098, 1874, 1982, 2256
Tru9I TTAA 8 cut(s) 41, 207, 618, 693, 1098, 1874, 1982, 2256
TscAI CASTG 4 cut(s) 126, 1012, 1636, 2613
TseFI GTSAC 5 cut(s) 461, 506, 601, 1441, 1763
TseI GCWGC 6 cut(s) 129, 320, 654, 1268, 1526, 2588
Tsp45I GTSAC 5 cut(s) 461, 506, 601, 1441, 1763
TspGWI ACGGA 2 cut(s) 376, 644
TspRI CASTG 4 cut(s) 126, 1012, 1636, 2613
Van91I CCANNNNNTGG 1 cut(s) 378
Vha464I CTTAAG 1 cut(s) 2255
VpaK11BI GGWCC 1 cut(s) 2153
XapI RAATTY 4 cut(s) 223, 1167, 1899, 2073
XceI RCATGY 4 cut(s) 421, 499, 729, 2057
XmiI GTMKAC 1 cut(s) 2319
ZraI GACGTC 1 cut(s) 1768
ZrmI AGTACT 2 cut(s) 2096, 2253
Zsp2I ATGCAT 2 cut(s) 24, 2059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.