Rroxscaffold_1G00073980

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000066
Physical Location & Seq
Reverse (-)
94909237 .. 94915225
5989 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00073980.1

Sequence Viewer

Length: 2316 bp
ATGGTCTTATACCGGAGAATGGTCATTTCGCAGCTGATTTCATTGGTAGTGGTGGCCATAGCTGCAGTAGCATTAGCCGACAAAGCCCTCCCGCTTCCCCATGCCCTGCCTAGTTGCCCTGACCACTGTGGCAATGTCAGAGTCCCCTATCCATTCGGCATAGGCGATGGTTGTTACCTTATACAAAAATTCAACCTCACCTGTGACGAATCCAGCCAACCTCCAACACTACACCGGACGGATGGTAGTCTCATTATTACTAACATATCCGTGGCCGAAGGTGAGTCGCAAGTTTTGAATCACATAGCCTATGATTGTTACGACCAACAGGGTCATCAAACGGAAAGCATGTACAATTCCCCATCGCTCTGGCTCACTCCTCCGTTCACCATCTCTGGTACCAGAAACAAGTTCTTTGCGGTTGGCTATGACACCTATGCCATTTTTACAGGGTACCGCCCTTACCCTAATGATGGTGCTAGGTACGTATCCGGGTGCATGTCCATAAGTGACAATCCTGACACTACTACTCCTGCTGCTAACGAGTCATGCACCGGCATTGGGTGTTGTCAAATTCCAATCCCTAACGGACTCAAAAATCGTACTATCCAGTTGAGTAGTTATTCTAAGCATACCTATGTATGGAAATTCAACCCGTGCAGCTACGCTTTCATTGTAGAAGAAGGCAATTTCACATTTAACCCGAATACAAGTTTTCAACTACTGAGAAACACTGAACAGCTTCCTCTGATTCTTAATTGGGAAATTGCGGACGGGTCATGTGCTGAAGCTCAAAAGAGGCTGGATTATGCTTGCAAGGCGCATAGCAAGTGTGTCAACCGGACCATCGACATCAAGACTGAACCGTCTGGTTATTATTGCCAGTGCTTGCCAGGCTACCAAGGGAACCCATATCTCCCAGATGGTTGTCAAGATATTGATGAGTGCATCGCTTCAACGAACCCCTGCAACAATGGAACGTGCACAAATTCAGATGGAGATTACACATGTACATGTAACAAAGGTTTCAGAAACGAAAACCCAAATAGTTGCATACAGAACACTCCTATATCAAAGAACAAGTCCCTGAAAGTTTCCTTGGGTGTCTCTCTGAGCTTCTTAGGGGTATTGCTTGTAACATTTTGGATATACTGCGGACTGAAGAGAAGAAAGTTCAAGATACTCAAAGAGAAGTACTTCAAACACAATGGTGGCTTCTTGTTACAACAAAAATTGGAGAATTTCGATGGACCTCAAGCAGCCAAAATATTTACTAGAGAAGAACTTAAGAAGGCCACAAACAACTTTCATGACAATTTAAAAATTGGTGAAGGAGGCTATGGATTTGTTTACAAAGGAACACTATCAAATGGAAATGAGGTTGCCATAAAGGTGTCAAAAAGTAGTGCCCCAATGACCCAGAGTAATCAGTTCATTAATGAGCTGATTGTTCTTTCTCAGATCAACCACAGAAATGTGGTGAAGCTTCTAGGTTGTTGTTTGGAGACCCAAACGCCTCTATTAGTGTATGAGTTCGTCAGCAACGGCACACTTTATGAGCATATTCATAAAAAGAATGGCAAGGGACCGCTTTCCTTTGGGCTACGAATGAAGATAGCAACAGAAACAGCAGGATCACTGGCATACTTGCACTACTCTACTTCGATGCAAATTGTGCACCGAGATGTAAAGGCAGCAAACATTCTACTGGATGAGAATTTCACAGCGAAAGTGTCAGACTTTGGAGCTTCAAAATTGGTTCCCGAAGATCAAAATCAACCGTCTACTTTAGTGCAAGGGACGATAGGGTACTTGGACCCTGAATATCTTCAGTCAAACACACTGACAGAGAAAAGTGACGTATATAGCTTCGGCGTTGTCCTTGTGGAGCTAATAACAAGTCAAGTTGCAATTTCTTATCAGAAGCCTGAGGCGGAGAGAAGCCTAGCAAAATTTTTTGTTACTTCTGTGGAAGAGAATCGCCTAGATCAGATTCTCGATCATGAAATAATCAAAGAGGGGAGCTTCGAAACAGCTGAACAAGTAGCACTTCTGGCCAAAAGTTGTTTAAGTTTGAAAGGGAGCGATAGGCCTACCATGAAAGAAGTAGCAATGAAGCTAGAGGGCGTATTGCAAGTTATGGCAAAGCATCCATGGGGAAAGGCTGATGCCGACTACTTGCAGCTTGCGTCACATTCAAAAGCCTACGTTGTTGATGTTAGAGGTGATGAAGGTGAAGTTATAACCAGTATTGACTACGACCGGAGCATCCAGATTCAACCCCTGATGATGAAGCCTTGCGATGGAGGCCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK cEGF

Protein Analysis

771

Amino Acids

85.44

Weight (kDa)

6.3

Isoelectric Point (pI)

33.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 39 - 95 4.8e-12 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 312 - 351 4.2e-07 Calcium-binding EGF domain
Pkinase PF00069 437 - 704 1.4e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 441 - 707 1.4e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2243
AasI GACNNNNNNGTC 1 cut(s) 865
Acc65I GGTACC 2 cut(s) 398, 453
AccB1I GGYRCC 2 cut(s) 398, 453
AccI GTMKAC 1 cut(s) 1784
AciI CCGC 7 cut(s) 92, 419, 457, 770, 1155, 1589, 1934
AclWI GGATC 1 cut(s) 1642
AcoI YGGCCR 3 cut(s) 54, 273, 2055
AcsI RAATTY 7 cut(s) 188, 573, 647, 988, 1240, 1717, 1952
AcuI CTGAAG 3 cut(s) 807, 1181, 1814
AfaI GTAC 8 cut(s) 353, 400, 455, 485, 604, 1012, 1196, 1811
AfiI CCNNNNNNNGG 5 cut(s) 19, 473, 561, 642, 2303
AflII CTTAAG 1 cut(s) 1286
AflIII ACRYGT 2 cut(s) 1007, 1013
AjnI CCWGG 1 cut(s) 892
AjuI GAANNNNNNNTTGG 2 cut(s) 1082, 1114
AleI CACNNNNGTG 1 cut(s) 1209
Alw21I GWGCWC 2 cut(s) 986, 1680
Alw26I GTCTC 3 cut(s) 254, 1111, 1499
Alw44I GTGCAC 2 cut(s) 982, 1676
AlwI GGATC 1 cut(s) 1642
AoxI GGCC 6 cut(s) 54, 273, 1293, 2055, 2090, 2308
ApaLI GTGCAC 2 cut(s) 982, 1676
ApeKI GCWGC 7 cut(s) 31, 62, 536, 660, 1259, 1694, 2182
ApoI RAATTY 7 cut(s) 188, 573, 647, 988, 1240, 1717, 1952
ArsI GACNNNNNNTTYG 2 cut(s) 1067, 1099
AseI ATTAAT 1 cut(s) 1437
Asp700I GAANNNNTTC 3 cut(s) 741, 1196, 1827
Asp718I GGTACC 2 cut(s) 398, 453
AspLEI GCGC 1 cut(s) 823
AspS9I GGNCC 4 cut(s) 843, 1250, 1586, 1816
AsuC2I CCSGG 1 cut(s) 493
AsuHPI GGTGA 7 cut(s) 190, 293, 379, 1340, 1492, 2237, 2246
AsuII TTCGAA 1 cut(s) 2028
AvaII GGWCC 4 cut(s) 843, 1250, 1586, 1816
AxyI CCTNAGG 1 cut(s) 1929
BaeGI GKGCMC 3 cut(s) 986, 1411, 1680
BalI TGGCCA 2 cut(s) 56, 2057
BanI GGYRCC 2 cut(s) 398, 453
Bbv12I GWGCWC 2 cut(s) 986, 1680
BbvI GCAGC 7 cut(s) 43, 49, 523, 672, 1271, 1706, 2194
BceAI ACGGC 1 cut(s) 1561
BciT130I CCWGG 1 cut(s) 894
BciVI GTATCC 1 cut(s) 499
BcnI CCSGG 1 cut(s) 493
BcoDI GTCTC 3 cut(s) 254, 1111, 1499
BfaI CTAG 7 cut(s) 111, 480, 1275, 1490, 1946, 1985, 2120
BfmI CTRYAG 1 cut(s) 63
BfrI CTTAAG 1 cut(s) 1286
BfuI GTATCC 1 cut(s) 499
BisI GCNGC 7 cut(s) 32, 63, 537, 661, 1260, 1695, 2183
BlsI GCNGC 7 cut(s) 33, 64, 538, 662, 1261, 1696, 2184
BmcAI AGTACT 1 cut(s) 1196
Bme1390I CCNGG 2 cut(s) 493, 894
Bme18I GGWCC 4 cut(s) 843, 1250, 1586, 1816
BmgT120I GGNCC 4 cut(s) 843, 1250, 1586, 1816
BmiI GGNNCC 6 cut(s) 400, 455, 908, 1587, 1761, 1818
BmrFI CCNGG 2 cut(s) 493, 894
BmsI GCATC 5 cut(s) 957, 1656, 2158, 2158, 2277
Bpu14I TTCGAA 1 cut(s) 2028
BpuEI CTTGAG 1 cut(s) 1239
BpuMI CCSGG 1 cut(s) 493
BsaAI YACGTR 1 cut(s) 487
BsaBI GATNNNNATC 1 cut(s) 1773
BsaI GGTCTC 1 cut(s) 1499
BsaJI CCNNGG 4 cut(s) 270, 901, 1098, 2153
BsaWI WCCGGW 4 cut(s) 12, 234, 840, 2262
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bsc4I CCNNNNNNNGG 5 cut(s) 19, 473, 561, 642, 2303
Bse118I RCCGGY 1 cut(s) 554
Bse1I ACTGG 5 cut(s) 610, 883, 1644, 1713, 2247
Bse21I CCTNAGG 1 cut(s) 1929
Bse3DI GCAATG 2 cut(s) 139, 2118
Bse8I GATNNNNATC 1 cut(s) 1773
BseBI CCWGG 1 cut(s) 894
BseDI CCNNGG 4 cut(s) 270, 901, 1098, 2153
BseGI GGATG 4 cut(s) 247, 1717, 2149, 2268
BseJI GATNNNNATC 1 cut(s) 1773
BseLI CCNNNNNNNGG 5 cut(s) 19, 473, 561, 642, 2303
BseMI GCAATG 2 cut(s) 139, 2118
BseMII CTCAG 4 cut(s) 716, 1103, 1472, 1920
BseNI ACTGG 5 cut(s) 610, 883, 1644, 1713, 2247
BseRI GAGGAG 1 cut(s) 369
BseSI GKGCMC 3 cut(s) 986, 1411, 1680
BseXI GCAGC 7 cut(s) 43, 49, 523, 672, 1271, 1706, 2194
BsgI GTGCAG 1 cut(s) 679
Bsh1285I CGRYCG 1 cut(s) 2263
BshFI GGCC 6 cut(s) 56, 275, 1295, 2057, 2092, 2310
BshNI GGYRCC 2 cut(s) 398, 453
BsiEI CGRYCG 1 cut(s) 2263
BsiHKAI GWGCWC 2 cut(s) 986, 1680
BsiSI CCGG 6 cut(s) 13, 235, 492, 555, 841, 2263
BslFI GGGAC 4 cut(s) 128, 1069, 1599, 1813
BslI CCNNNNNNNGG 5 cut(s) 19, 473, 561, 642, 2303
BsmAI GTCTC 3 cut(s) 254, 1111, 1499
BsmFI GGGAC 4 cut(s) 128, 1069, 1599, 1813
BsnI GGCC 6 cut(s) 56, 275, 1295, 2057, 2092, 2310
Bso31I GGTCTC 1 cut(s) 1499
Bsp119I TTCGAA 1 cut(s) 2028
Bsp1286I GDGCHC 3 cut(s) 986, 1411, 1680
Bsp1407I TGTACA 2 cut(s) 351, 1010
Bsp143I GATC 5 cut(s) 1461, 1634, 1768, 1987, 1999
Bsp19I CCATGG 1 cut(s) 2153
BspACI CCGC 7 cut(s) 92, 419, 457, 770, 1155, 1589, 1934
BspANI GGCC 6 cut(s) 56, 275, 1295, 2057, 2092, 2310
BspCNI CTCAG 4 cut(s) 717, 1104, 1471, 1921
BspHI TCATGA 2 cut(s) 1309, 2002
BspLI GGNNCC 6 cut(s) 400, 455, 908, 1587, 1761, 1818
BspMAI CTGCAG 1 cut(s) 67
BspPI GGATC 1 cut(s) 1642
BspT104I TTCGAA 1 cut(s) 2028
BspT107I GGYRCC 2 cut(s) 398, 453
BspTI CTTAAG 1 cut(s) 1286
BspTNI GGTCTC 1 cut(s) 1499
BsrDI GCAATG 2 cut(s) 139, 2118
BsrFI RCCGGY 1 cut(s) 554
BsrGI TGTACA 2 cut(s) 351, 1010
BsrI ACTGG 5 cut(s) 610, 883, 1644, 1713, 2247
BssAI RCCGGY 1 cut(s) 554
BssECI CCNNGG 4 cut(s) 270, 901, 1098, 2153
BssMI GATC 5 cut(s) 1461, 1634, 1768, 1987, 1999
BssT1I CCWWGG 3 cut(s) 901, 1098, 2153
Bst2UI CCWGG 1 cut(s) 894
Bst4CI ACNGT 3 cut(s) 128, 867, 1782
Bst6I CTCTTC 2 cut(s) 1157, 1968
BstAFI CTTAAG 1 cut(s) 1286
BstAPI GCANNNNNTGC 1 cut(s) 1675
BstAUI TGTACA 2 cut(s) 351, 1010
BstBAI YACGTR 1 cut(s) 487
BstBI TTCGAA 1 cut(s) 2028
BstC8I GCNNGC 3 cut(s) 814, 890, 2187
BstDEI CTNAG 6 cut(s) 627, 725, 1112, 1120, 1458, 1929
BstDSI CCRYGG 2 cut(s) 270, 2153
BstF5I GGATG 4 cut(s) 247, 1717, 2149, 2268
BstHHI GCGC 1 cut(s) 823
BstKTI GATC 5 cut(s) 1464, 1637, 1771, 1990, 2002
BstMAI GTCTC 3 cut(s) 254, 1111, 1499
BstMBI GATC 5 cut(s) 1461, 1634, 1768, 1987, 1999
BstMCI CGRYCG 1 cut(s) 2263
BstMWI GCNNNNNNNGC 8 cut(s) 62, 68, 83, 818, 894, 1675, 2054, 2307
BstNI CCWGG 1 cut(s) 894
BstNSI RCATGY 4 cut(s) 352, 502, 1011, 1017
BstSCI CCNGG 2 cut(s) 491, 892
BstSFI CTRYAG 1 cut(s) 63
BstSLI GKGCMC 3 cut(s) 986, 1411, 1680
BstSNI TACGTA 1 cut(s) 487
BstV1I GCAGC 7 cut(s) 43, 49, 523, 672, 1271, 1706, 2194
BstXI CCANNNNNNTGG 1 cut(s) 369
Bsu36I CCTNAGG 1 cut(s) 1929
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 6 cut(s) 56, 275, 1295, 2057, 2092, 2310
BtgI CCRYGG 2 cut(s) 270, 2153
BtgZI GCGATG 3 cut(s) 180, 348, 934
BtsCI GGATG 4 cut(s) 247, 1717, 2149, 2268
BtsIMutI CAGTG 5 cut(s) 124, 732, 890, 1637, 1841
Cac8I GCNNGC 3 cut(s) 814, 890, 2187
CciI TCATGA 2 cut(s) 1309, 2002
CfoI GCGC 1 cut(s) 823
Cfr10I RCCGGY 1 cut(s) 554
Cfr13I GGNCC 4 cut(s) 843, 1250, 1586, 1816
CseI GACGC 1 cut(s) 2178
Csp6I GTAC 8 cut(s) 352, 399, 454, 484, 603, 1011, 1195, 1810
CviQI GTAC 8 cut(s) 352, 399, 454, 484, 603, 1011, 1195, 1810
DdeI CTNAG 6 cut(s) 627, 725, 1112, 1120, 1458, 1929
DpnI GATC 5 cut(s) 1463, 1636, 1770, 1989, 2001
DpnII GATC 5 cut(s) 1461, 1634, 1768, 1987, 1999
DraI TTTAAA 1 cut(s) 1320
DrdI GACNNNNNNGTC 1 cut(s) 865
DseDI GACNNNNNNGTC 1 cut(s) 865
EaeI YGGCCR 3 cut(s) 54, 273, 2055
Eam1104I CTCTTC 2 cut(s) 1157, 1968
EarI CTCTTC 2 cut(s) 1157, 1968
EciI GGCGGA 1 cut(s) 1949
Eco105I TACGTA 1 cut(s) 487
Eco130I CCWWGG 3 cut(s) 901, 1098, 2153
Eco147I AGGCCT 1 cut(s) 2092
Eco31I GGTCTC 1 cut(s) 1499
Eco47I GGWCC 4 cut(s) 843, 1250, 1586, 1816
Eco57I CTGAAG 3 cut(s) 807, 1181, 1814
Eco81I CCTNAGG 1 cut(s) 1929
EcoRII CCWGG 1 cut(s) 892
EcoT14I CCWWGG 3 cut(s) 901, 1098, 2153
ErhI CCWWGG 3 cut(s) 901, 1098, 2153
FalI AAGNNNNNCTT 2 cut(s) 2034, 2066
FaqI GGGAC 4 cut(s) 128, 1069, 1599, 1813
FauI CCCGC 1 cut(s) 99
FblI GTMKAC 1 cut(s) 1784
Fnu4HI GCNGC 7 cut(s) 32, 63, 537, 661, 1260, 1695, 2183
FokI GGATG 4 cut(s) 254, 1724, 2136, 2255
Fsp4HI GCNGC 7 cut(s) 32, 63, 537, 661, 1260, 1695, 2183
FspBI CTAG 7 cut(s) 111, 480, 1275, 1490, 1946, 1985, 2120
GlaI GCGC 1 cut(s) 822
GluI GCNGC 7 cut(s) 32, 63, 537, 661, 1260, 1695, 2183
HaeIII GGCC 6 cut(s) 56, 275, 1295, 2057, 2092, 2310
HapII CCGG 6 cut(s) 13, 235, 492, 555, 841, 2263
HgaI GACGC 1 cut(s) 2178
HhaI GCGC 1 cut(s) 823
Hin6I GCGC 1 cut(s) 821
HinP1I GCGC 1 cut(s) 821
HincII GTYRAC 1 cut(s) 838
HindII GTYRAC 1 cut(s) 838
HindIII AAGCTT 1 cut(s) 1484
HpaII CCGG 6 cut(s) 13, 235, 492, 555, 841, 2263
HphI GGTGA 7 cut(s) 190, 293, 379, 1340, 1492, 2237, 2246
Hpy166II GTNNAC 6 cut(s) 387, 838, 984, 1351, 1678, 1785
Hpy188I TCNGA 9 cut(s) 140, 750, 994, 1031, 1113, 1461, 1738, 1923, 1992
Hpy188III TCNNGA 9 cut(s) 518, 856, 932, 1177, 1310, 1763, 1997, 2003, 2272
Hpy8I GTNNAC 6 cut(s) 387, 838, 984, 1351, 1678, 1785
HpyAV CCTTC 5 cut(s) 272, 677, 1285, 1325, 2225
HpyCH4III ACNGT 3 cut(s) 128, 867, 1782
HpyCH4IV ACGT 4 cut(s) 486, 980, 1860, 2208
HpyF10VI GCNNNNNNNGC 8 cut(s) 62, 68, 83, 818, 894, 1675, 2054, 2307
HpyF3I CTNAG 6 cut(s) 627, 725, 1112, 1120, 1458, 1929
HpySE526I ACGT 4 cut(s) 486, 980, 1860, 2208
HspAI GCGC 1 cut(s) 821
KpnI GGTACC 2 cut(s) 402, 457
Kzo9I GATC 5 cut(s) 1461, 1634, 1768, 1987, 1999
LmnI GCTCC 5 cut(s) 1745, 1888, 2022, 2082, 2265
Lsp1109I GCAGC 7 cut(s) 43, 49, 523, 672, 1271, 1706, 2194
LweI GCATC 5 cut(s) 957, 1656, 2158, 2158, 2277
MaeI CTAG 7 cut(s) 111, 480, 1275, 1490, 1946, 1985, 2120
MaeII ACGT 4 cut(s) 486, 980, 1860, 2208
MalI GATC 5 cut(s) 1463, 1636, 1770, 1989, 2001
MboI GATC 5 cut(s) 1461, 1634, 1768, 1987, 1999
MboII GAAGA 8 cut(s) 692, 1174, 1179, 1292, 1624, 1778, 1820, 1985
MhlI GDGCHC 3 cut(s) 986, 1411, 1680
MlsI TGGCCA 2 cut(s) 56, 2057
MluNI TGGCCA 2 cut(s) 56, 2057
MlyI GAGTC 4 cut(s) 150, 293, 554, 585
MmeI TCCRAC 1 cut(s) 248
Mox20I TGGCCA 2 cut(s) 56, 2057
MroXI GAANNNNTTC 3 cut(s) 741, 1196, 1827
MscI TGGCCA 2 cut(s) 56, 2057
MseI TTAA 6 cut(s) 699, 756, 1287, 1319, 1437, 2069
MslI CAYNNNNRTG 7 cut(s) 269, 636, 1012, 1209, 1391, 1473, 1683
Msp20I TGGCCA 2 cut(s) 56, 2057
MspA1I CMGCKG 2 cut(s) 34, 2036
MspCI CTTAAG 1 cut(s) 1286
MspI CCGG 6 cut(s) 13, 235, 492, 555, 841, 2263
MspR9I CCNGG 2 cut(s) 493, 894
MvaI CCWGG 1 cut(s) 894
MwoI GCNNNNNNNGC 8 cut(s) 62, 68, 83, 818, 894, 1675, 2054, 2307
NciI CCSGG 1 cut(s) 493
NcoI CCATGG 1 cut(s) 2153
NdeII GATC 5 cut(s) 1461, 1634, 1768, 1987, 1999
NlaIV GGNNCC 6 cut(s) 400, 455, 908, 1587, 1761, 1818
NmuCI GTSAC 4 cut(s) 203, 509, 1856, 2190
NspI RCATGY 4 cut(s) 352, 502, 1011, 1017
NspV TTCGAA 1 cut(s) 2028
OliI CACNNNNGTG 1 cut(s) 1209
PagI TCATGA 2 cut(s) 1309, 2002
PceI AGGCCT 1 cut(s) 2092
PciI ACATGT 2 cut(s) 1007, 1013
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PdmI GAANNNNTTC 3 cut(s) 741, 1196, 1827
PfeI GAWTC 6 cut(s) 209, 298, 751, 1978, 1993, 2275
PkrI GCNGC 7 cut(s) 33, 64, 538, 662, 1261, 1696, 2184
PleI GAGTC 4 cut(s) 149, 292, 553, 585
PpsI GAGTC 4 cut(s) 149, 292, 553, 585
Ppu21I YACGTR 1 cut(s) 487
PscI ACATGT 2 cut(s) 1007, 1013
PshBI ATTAAT 1 cut(s) 1437
PsiI TTATAA 1 cut(s) 2243
Psp6I CCWGG 1 cut(s) 892
PspGI CCWGG 1 cut(s) 892
PspN4I GGNNCC 6 cut(s) 400, 455, 908, 1587, 1761, 1818
PspPI GGNCC 4 cut(s) 843, 1250, 1586, 1816
PstI CTGCAG 1 cut(s) 67
PvuII CAGCTG 2 cut(s) 34, 2036
RsaI GTAC 8 cut(s) 353, 400, 455, 485, 604, 1012, 1196, 1811
RsaNI GTAC 8 cut(s) 352, 399, 454, 484, 603, 1011, 1195, 1810
RseI CAYNNNNRTG 7 cut(s) 269, 636, 1012, 1209, 1391, 1473, 1683
SaqAI TTAA 6 cut(s) 699, 756, 1287, 1319, 1437, 2069
SatI GCNGC 7 cut(s) 32, 63, 537, 661, 1260, 1695, 2183
Sau3AI GATC 5 cut(s) 1461, 1634, 1768, 1987, 1999
Sau96I GGNCC 4 cut(s) 843, 1250, 1586, 1816
ScaI AGTACT 1 cut(s) 1196
SchI GAGTC 4 cut(s) 150, 293, 554, 585
ScrFI CCNGG 2 cut(s) 493, 894
SduI GDGCHC 3 cut(s) 986, 1411, 1680
SfaNI GCATC 5 cut(s) 957, 1656, 2158, 2158, 2277
SfcI CTRYAG 1 cut(s) 63
SfuI TTCGAA 1 cut(s) 2028
SinI GGWCC 4 cut(s) 843, 1250, 1586, 1816
SmiMI CAYNNNNRTG 7 cut(s) 269, 636, 1012, 1209, 1391, 1473, 1683
SmlI CTYRAG 2 cut(s) 1254, 1286
SmoI CTYRAG 2 cut(s) 1254, 1286
SnaBI TACGTA 1 cut(s) 487
SseBI AGGCCT 1 cut(s) 2092
SsiI CCGC 7 cut(s) 92, 419, 457, 770, 1155, 1589, 1934
SspI AATATT 1 cut(s) 1269
SspMI CTAG 7 cut(s) 111, 480, 1275, 1490, 1946, 1985, 2120
StuI AGGCCT 1 cut(s) 2092
StyD4I CCNGG 2 cut(s) 491, 892
StyI CCWWGG 3 cut(s) 901, 1098, 2153
TaaI ACNGT 3 cut(s) 128, 867, 1782
TaiI ACGT 4 cut(s) 489, 983, 1863, 2211
TaqI TCGA 5 cut(s) 849, 1245, 1664, 1998, 2028
TatI WGTACW 3 cut(s) 351, 1010, 1194
TfiI GAWTC 6 cut(s) 209, 298, 751, 1978, 1993, 2275
Tru1I TTAA 6 cut(s) 699, 756, 1287, 1319, 1437, 2069
Tru9I TTAA 6 cut(s) 699, 756, 1287, 1319, 1437, 2069
TscAI CASTG 5 cut(s) 131, 739, 890, 1644, 1848
TseFI GTSAC 4 cut(s) 203, 509, 1856, 2190
TseI GCWGC 7 cut(s) 31, 62, 536, 660, 1259, 1694, 2182
Tsp45I GTSAC 4 cut(s) 203, 509, 1856, 2190
TspGWI ACGGA 5 cut(s) 254, 259, 356, 372, 603
TspRI CASTG 5 cut(s) 131, 739, 890, 1644, 1848
Vha464I CTTAAG 1 cut(s) 1286
VneI GTGCAC 2 cut(s) 982, 1676
VpaK11BI GGWCC 4 cut(s) 843, 1250, 1586, 1816
VspI ATTAAT 1 cut(s) 1437
XapI RAATTY 7 cut(s) 188, 573, 647, 988, 1240, 1717, 1952
XceI RCATGY 4 cut(s) 352, 502, 1011, 1017
XmiI GTMKAC 1 cut(s) 1784
XmnI GAANNNNTTC 3 cut(s) 741, 1196, 1827
XspI CTAG 7 cut(s) 111, 480, 1275, 1490, 1946, 1985, 2120
ZrmI AGTACT 1 cut(s) 1196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.