Rroxscaffold_1G00060140

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000066
Physical Location & Seq
Forward (+)
82281398 .. 82284346
2949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00060140.1

Sequence Viewer

Length: 1731 bp
ATGTCCCTATGCAACAGGCTTGGCAATGTCGATAACTCTTGCTCCGGAGTTGGGTGTTGCCAGATTAACATCCCCAGAGGGCTGTACAATCTGACGCTGGCAGTGGATAGCTTCAAGAATCATTCGGGTATATGGAACTTTAACCCCTGCGGCTACGCCTTCATTGCAGAGCAAGGCCATTTCAATTTCTCCCGTGCGAGTTTTGATGAATTGAACAACACTGAACAGATTCCACTGGTTGCTAATTCGGCAATTGGGAAAGACAAAGACCCCTGCGATGAAGCTCAAAAGAGGAAGGATTTCACTTGCAAGGCTAAAAGCAGGTGCTTCAACAGGCCTGACAATGGGTCCATAAGTTACTTGTGCCAATGCTTGCCTGGTTACGAAGGAAACCCTTACCATCCAGATGGTTGCCAAGATATTGATGTGTGCAAGGCTCCAAACCTCTGCCAGCATGGAAAGTGCTCAAATTCACCAGGAAATTACTCTTGTTCATGTCACAAAGGTTACAAACATGACCGCATGAATGACAAAATTTGCGTGAAGGACGATCCCCTGCTTATTGTTCTATTGGGTGTAAGTGTAGGCTTCTTGGTTTTATTTGTTGGAATTTCATGGTTATGGTGGGGAAATAAGAAAATACAGTTCATCAAATTGAAAGAAAAGTACTTCAAAGAAAATGGAGGCATATTATTACAACAACAACTTGCCAGCCATGGAGGTTCCGTCGACACAACAAAAATTTATAGTGCAGAAGAACTTCAGAAGGCTACAAACAATTACCATGAAAGTAGAGTTCTTGGCAAAGGAGGCTATGGAATAGTATACAAAGGTATACTACCAGATAACCAAGAGATTGCCATAAAGAAGTCAAAAGTTTGTGCCGGAAGTCAGAGTGAGCAATTTGTCAACGAGGTAATCATTCTTTCTCAAATCAACCACAAAAATGTTGTGAAGCTTTTAGGTTGTTGTTTAGAAACAGAAGTGCCTATACTTGTATACGAGTTCATCAGCCATGGCACTCTTCATGAGCACATTCATAAGAAAAGATCATCACTCTCATTTGAATTACGAATGAAGATAGCAGCTGAAGCAGCAAGAGCACTAGCCCATTTACACTTCTCAATTTCCACACCAATCATACATCGAGATGTCAAAGCAGCGAATATTCCGTTAGATGATAAGTACACGGCGAAAGTGTCAGACTTTGGAGCTTCACGATTGGTTTCTTCAGGATACCTGGACCCTGAATATCTGCAATCAAACCAACTAACAGAAAAGAGTGACGTTTACAGCTTTGGGGTTCTCTTGGCTGAGCTTTTAACAAGCAAAGTGGCAGTTTCTAAAGATAAATTCTTAGCAAGCATCTTTGTTGCTTGCATGGAAAAAGATTGCTTAAATCAAGTGCTTGATGATGAGATAGTGAATGAAGGAAATATTGAGATGGCAAAAAATGTGGCCAAACTTGCAAGAAGATGTTTGAGGGTAAAAGGGGAGGAAAGGCCTACCATGAAAGAAGTAGCCATGGAGCTAGAAGGAATGATTATGACAAAGCATCCATGGGGTTCTGCAGATCATAACTTTCCAGAAGAGACTAAGCACTTGCTCGGATCACCTAATACTTCCAAGGATTACTCTGTGAATGTTGATGGTGGTGGTGGTCCTGGTACTGCCAGTGGGTCCAGCATGCAAATAGACATGTCAATGTCTTCATATGCTGATGGGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

576

Amino Acids

63.62

Weight (kDa)

7.0

Isoelectric Point (pI)

32.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 261 - 507 4.8e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 264 - 511 1.1e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 312
Acc36I ACCTGC 1 cut(s) 312
AccI GTMKAC 4 cut(s) 729, 825, 835, 999
AccIII TCCGGA 1 cut(s) 44
AciI CCGC 2 cut(s) 150, 520
AclWI GGATC 2 cut(s) 545, 1618
AcoI YGGCCR 1 cut(s) 1458
AcsI RAATTY 5 cut(s) 469, 534, 609, 741, 1352
AcuI CTGAAG 3 cut(s) 746, 1110, 1215
AfaI GTAC 4 cut(s) 86, 668, 1187, 1669
AfiI CCNNNNNNNGG 2 cut(s) 51, 344
AflIII ACRYGT 1 cut(s) 1698
AgsI TTSAA 7 cut(s) 115, 184, 214, 331, 658, 673, 1067
AhdI GACNNNNNGTC 1 cut(s) 346
AjnI CCWGG 4 cut(s) 376, 475, 1239, 1663
AluBI AGCT 8 cut(s) 111, 284, 958, 1088, 1214, 1296, 1318, 1531
AluI AGCT 8 cut(s) 111, 284, 958, 1088, 1214, 1296, 1318, 1531
Alw21I GWGCWC 3 cut(s) 467, 1035, 1105
Alw26I GTCTC 1 cut(s) 1586
AlwI GGATC 2 cut(s) 545, 1618
Aor13HI TCCGGA 1 cut(s) 44
AoxI GGCC 4 cut(s) 175, 335, 1458, 1502
ApeKI GCWGC 3 cut(s) 1085, 1094, 1160
ApoI RAATTY 5 cut(s) 469, 534, 609, 741, 1352
Asp700I GAANNNNTTC 3 cut(s) 228, 299, 759
AspS9I GGNCC 4 cut(s) 348, 1243, 1661, 1680
AsuHPI GGTGA 2 cut(s) 465, 1605
AvaII GGWCC 4 cut(s) 348, 1243, 1661, 1680
BalI TGGCCA 1 cut(s) 1460
BarI GAAGNNNNNNTAC 2 cut(s) 975, 1007
BbsI GAAGAC 1 cut(s) 1701
Bbv12I GWGCWC 3 cut(s) 467, 1035, 1105
BbvI GCAGC 3 cut(s) 1097, 1106, 1172
BccI CCATC 5 cut(s) 401, 408, 1438, 1643, 1715
BceAI ACGGC 1 cut(s) 1206
BciT130I CCWGG 4 cut(s) 378, 477, 1241, 1665
BciVI GTATCC 1 cut(s) 1229
BcoDI GTCTC 1 cut(s) 1586
BfaI CTAG 2 cut(s) 1106, 1532
BfmI CTRYAG 1 cut(s) 1569
BfuAI ACCTGC 1 cut(s) 312
BfuI GTATCC 1 cut(s) 1229
BisI GCNGC 4 cut(s) 151, 1086, 1095, 1161
BlpI GCTNAGC 1 cut(s) 1314
BlsI GCNGC 4 cut(s) 152, 1087, 1096, 1162
BmcAI AGTACT 1 cut(s) 668
Bme1390I CCNGG 4 cut(s) 378, 477, 1241, 1665
Bme18I GGWCC 4 cut(s) 348, 1243, 1661, 1680
BmeRI GACNNNNNGTC 1 cut(s) 346
BmgT120I GGNCC 4 cut(s) 348, 1243, 1661, 1680
BmiI GGNNCC 5 cut(s) 349, 438, 724, 1245, 1681
BmrFI CCNGG 4 cut(s) 378, 477, 1241, 1665
BmsI GCATC 2 cut(s) 1374, 1564
BpiI GAAGAC 1 cut(s) 1701
Bpu1102I GCTNAGC 1 cut(s) 1314
BsaBI GATNNNNATC 1 cut(s) 68
BsaJI CCNNGG 5 cut(s) 715, 1015, 1524, 1559, 1626
BsaWI WCCGGW 1 cut(s) 44
BsaXI ACNNNNNCTCC 4 cut(s) 26, 56, 711, 741
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 344
Bse1I ACTGG 2 cut(s) 240, 1674
Bse3DI GCAATG 2 cut(s) 31, 162
Bse8I GATNNNNATC 1 cut(s) 68
BseAI TCCGGA 1 cut(s) 44
BseBI CCWGG 4 cut(s) 378, 477, 1241, 1665
BseDI CCNNGG 5 cut(s) 715, 1015, 1524, 1559, 1626
BseGI GGATG 3 cut(s) 69, 400, 1555
BseJI GATNNNNATC 1 cut(s) 68
BseLI CCNNNNNNNGG 2 cut(s) 51, 344
BseMI GCAATG 2 cut(s) 31, 162
BseMII CTCAG 1 cut(s) 1305
BseNI ACTGG 2 cut(s) 240, 1674
BseXI GCAGC 3 cut(s) 1097, 1106, 1172
BsgI GTGCAG 1 cut(s) 771
BshFI GGCC 4 cut(s) 177, 337, 1460, 1504
BsiHKAI GWGCWC 3 cut(s) 467, 1035, 1105
BsiSI CCGG 2 cut(s) 45, 885
BslI CCNNNNNNNGG 2 cut(s) 51, 344
BsmAI GTCTC 1 cut(s) 1586
BsnI GGCC 4 cut(s) 177, 337, 1460, 1504
Bsp1286I GDGCHC 3 cut(s) 467, 1035, 1105
Bsp13I TCCGGA 1 cut(s) 44
Bsp1407I TGTACA 1 cut(s) 84
Bsp143I GATC 4 cut(s) 550, 1049, 1573, 1610
Bsp1720I GCTNAGC 1 cut(s) 1314
Bsp19I CCATGG 4 cut(s) 715, 1015, 1524, 1559
BspACI CCGC 2 cut(s) 150, 520
BspANI GGCC 4 cut(s) 177, 337, 1460, 1504
BspCNI CTCAG 1 cut(s) 1306
BspEI TCCGGA 1 cut(s) 44
BspHI TCATGA 1 cut(s) 1027
BspLI GGNNCC 5 cut(s) 349, 438, 724, 1245, 1681
BspMAI CTGCAG 1 cut(s) 1573
BspMI ACCTGC 1 cut(s) 312
BspPI GGATC 2 cut(s) 545, 1618
BsrDI GCAATG 2 cut(s) 31, 162
BsrGI TGTACA 1 cut(s) 84
BsrI ACTGG 2 cut(s) 240, 1674
BssECI CCNNGG 5 cut(s) 715, 1015, 1524, 1559, 1626
BssMI GATC 4 cut(s) 550, 1049, 1573, 1610
BssNAI GTATAC 3 cut(s) 826, 836, 1000
BssT1I CCWWGG 5 cut(s) 715, 1015, 1524, 1559, 1626
Bst1107I GTATAC 3 cut(s) 826, 836, 1000
Bst2UI CCWGG 4 cut(s) 378, 477, 1241, 1665
Bst4CI ACNGT 1 cut(s) 645
Bst6I CTCTTC 2 cut(s) 1029, 1584
BstAUI TGTACA 1 cut(s) 84
BstC8I GCNNGC 7 cut(s) 99, 374, 452, 712, 1363, 1378, 1688
BstDEI CTNAG 3 cut(s) 1314, 1357, 1596
BstDSI CCRYGG 4 cut(s) 715, 1015, 1524, 1559
BstF5I GGATG 3 cut(s) 69, 400, 1555
BstKTI GATC 4 cut(s) 553, 1052, 1576, 1613
BstMAI GTCTC 1 cut(s) 1586
BstMBI GATC 4 cut(s) 550, 1049, 1573, 1610
BstMWI GCNNNNNNNGC 7 cut(s) 164, 248, 810, 1091, 1094, 1100, 1466
BstNI CCWGG 4 cut(s) 378, 477, 1241, 1665
BstNSI RCATGY 2 cut(s) 1690, 1702
BstSCI CCNGG 4 cut(s) 376, 475, 1239, 1663
BstSFI CTRYAG 1 cut(s) 1569
BstV1I GCAGC 3 cut(s) 1097, 1106, 1172
BstV2I GAAGAC 1 cut(s) 1701
BstXI CCANNNNNNTGG 1 cut(s) 407
BstZ17I GTATAC 3 cut(s) 826, 836, 1000
BsuI GTATCC 1 cut(s) 1229
BsuRI GGCC 4 cut(s) 177, 337, 1460, 1504
BtgI CCRYGG 4 cut(s) 715, 1015, 1524, 1559
BtgZI GCGATG 1 cut(s) 291
BtsCI GGATG 3 cut(s) 69, 400, 1555
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 4 cut(s) 108, 219, 233, 1681
BveI ACCTGC 1 cut(s) 312
Cac8I GCNNGC 7 cut(s) 99, 374, 452, 712, 1363, 1378, 1688
CciI TCATGA 1 cut(s) 1027
Cfr13I GGNCC 4 cut(s) 348, 1243, 1661, 1680
CseI GACGC 1 cut(s) 103
Csp6I GTAC 4 cut(s) 85, 667, 1186, 1668
CspCI CAANNNNNGTGG 4 cut(s) 1314, 1349, 1437, 1472
CviQI GTAC 4 cut(s) 85, 667, 1186, 1668
DdeI CTNAG 3 cut(s) 1314, 1357, 1596
DpnI GATC 4 cut(s) 552, 1051, 1575, 1612
DpnII GATC 4 cut(s) 550, 1049, 1573, 1610
DriI GACNNNNNGTC 1 cut(s) 346
EaeI YGGCCR 1 cut(s) 1458
Eam1104I CTCTTC 2 cut(s) 1029, 1584
Eam1105I GACNNNNNGTC 1 cut(s) 346
EarI CTCTTC 2 cut(s) 1029, 1584
Eco130I CCWWGG 5 cut(s) 715, 1015, 1524, 1559, 1626
Eco147I AGGCCT 2 cut(s) 337, 1504
Eco47I GGWCC 4 cut(s) 348, 1243, 1661, 1680
Eco57I CTGAAG 3 cut(s) 746, 1110, 1215
EcoRII CCWGG 4 cut(s) 376, 475, 1239, 1663
EcoT14I CCWWGG 5 cut(s) 715, 1015, 1524, 1559, 1626
ErhI CCWWGG 5 cut(s) 715, 1015, 1524, 1559, 1626
FauNDI CATATG 1 cut(s) 1714
FblI GTMKAC 4 cut(s) 729, 825, 835, 999
Fnu4HI GCNGC 4 cut(s) 151, 1086, 1095, 1161
FokI GGATG 3 cut(s) 56, 387, 1542
Fsp4HI GCNGC 4 cut(s) 151, 1086, 1095, 1161
FspBI CTAG 2 cut(s) 1106, 1532
GluI GCNGC 4 cut(s) 151, 1086, 1095, 1161
HaeIII GGCC 4 cut(s) 177, 337, 1460, 1504
HapII CCGG 2 cut(s) 45, 885
HgaI GACGC 1 cut(s) 103
HincII GTYRAC 2 cut(s) 730, 910
HindII GTYRAC 2 cut(s) 730, 910
HindIII AAGCTT 1 cut(s) 956
HinfI GANTC 2 cut(s) 118, 229
HpaII CCGG 2 cut(s) 45, 885
HphI GGTGA 2 cut(s) 465, 1605
Hpy166II GTNNAC 7 cut(s) 730, 826, 836, 910, 1000, 1188, 1291
Hpy188I TCNGA 5 cut(s) 93, 765, 894, 1204, 1610
Hpy188III TCNNGA 8 cut(s) 45, 115, 404, 1028, 1148, 1218, 1233, 1586
Hpy8I GTNNAC 7 cut(s) 730, 826, 836, 910, 1000, 1188, 1291
Hpy99I CGWCG 1 cut(s) 731
HpyAV CCTTC 7 cut(s) 169, 289, 380, 538, 760, 1424, 1529
HpyCH4III ACNGT 1 cut(s) 645
HpyCH4IV ACGT 1 cut(s) 1287
HpyF10VI GCNNNNNNNGC 7 cut(s) 164, 248, 810, 1091, 1094, 1100, 1466
HpyF3I CTNAG 3 cut(s) 1314, 1357, 1596
HpySE526I ACGT 1 cut(s) 1287
Kpn2I TCCGGA 1 cut(s) 44
Kzo9I GATC 4 cut(s) 550, 1049, 1573, 1610
LmnI GCTCC 4 cut(s) 47, 442, 1211, 1528
Lsp1109I GCAGC 3 cut(s) 1097, 1106, 1172
LweI GCATC 2 cut(s) 1374, 1564
MaeI CTAG 2 cut(s) 1106, 1532
MaeII ACGT 1 cut(s) 1287
MaeIII GTNAC 5 cut(s) 356, 380, 497, 506, 1283
MalI GATC 4 cut(s) 552, 1051, 1575, 1612
MboI GATC 4 cut(s) 550, 1049, 1573, 1610
MboII GAAGA 7 cut(s) 767, 1016, 1090, 1221, 1485, 1601, 1701
MfeI CAATTG 1 cut(s) 252
MhlI GDGCHC 3 cut(s) 467, 1035, 1105
MlsI TGGCCA 1 cut(s) 1460
MluNI TGGCCA 1 cut(s) 1460
MmeI TCCRAC 1 cut(s) 586
MnlI CCTC 9 cut(s) 71, 285, 455, 677, 713, 803, 907, 1476, 1489
Mox20I TGGCCA 1 cut(s) 1460
MroI TCCGGA 1 cut(s) 44
MroXI GAANNNNTTC 3 cut(s) 228, 299, 759
MscI TGGCCA 1 cut(s) 1460
MseI TTAA 4 cut(s) 66, 141, 1322, 1397
MslI CAYNNNNRTG 5 cut(s) 405, 619, 945, 1149, 1703
Msp20I TGGCCA 1 cut(s) 1460
MspA1I CMGCKG 1 cut(s) 1088
MspI CCGG 2 cut(s) 45, 885
MspR9I CCNGG 4 cut(s) 378, 477, 1241, 1665
MunI CAATTG 1 cut(s) 252
MvaI CCWGG 4 cut(s) 378, 477, 1241, 1665
MwoI GCNNNNNNNGC 7 cut(s) 164, 248, 810, 1091, 1094, 1100, 1466
NcoI CCATGG 4 cut(s) 715, 1015, 1524, 1559
NdeI CATATG 1 cut(s) 1714
NdeII GATC 4 cut(s) 550, 1049, 1573, 1610
NlaIV GGNNCC 5 cut(s) 349, 438, 724, 1245, 1681
NmuCI GTSAC 2 cut(s) 497, 1283
NspI RCATGY 2 cut(s) 1690, 1702
PaeI GCATGC 1 cut(s) 1690
PagI TCATGA 1 cut(s) 1027
PaqCI CACCTGC 1 cut(s) 312
PceI AGGCCT 2 cut(s) 337, 1504
PciI ACATGT 1 cut(s) 1698
PdmI GAANNNNTTC 3 cut(s) 228, 299, 759
PfeI GAWTC 2 cut(s) 118, 229
PkrI GCNGC 4 cut(s) 152, 1087, 1096, 1162
PscI ACATGT 1 cut(s) 1698
Psp6I CCWGG 4 cut(s) 376, 475, 1239, 1663
PspGI CCWGG 4 cut(s) 376, 475, 1239, 1663
PspN4I GGNNCC 5 cut(s) 349, 438, 724, 1245, 1681
PspPI GGNCC 4 cut(s) 348, 1243, 1661, 1680
PstI CTGCAG 1 cut(s) 1573
PvuII CAGCTG 1 cut(s) 1088
RsaI GTAC 4 cut(s) 86, 668, 1187, 1669
RsaNI GTAC 4 cut(s) 85, 667, 1186, 1668
RseI CAYNNNNRTG 5 cut(s) 405, 619, 945, 1149, 1703
SalI GTCGAC 1 cut(s) 728
SaqAI TTAA 4 cut(s) 66, 141, 1322, 1397
SatI GCNGC 4 cut(s) 151, 1086, 1095, 1161
Sau3AI GATC 4 cut(s) 550, 1049, 1573, 1610
Sau96I GGNCC 4 cut(s) 348, 1243, 1661, 1680
ScaI AGTACT 1 cut(s) 668
ScrFI CCNGG 4 cut(s) 378, 477, 1241, 1665
SduI GDGCHC 3 cut(s) 467, 1035, 1105
SfaNI GCATC 2 cut(s) 1374, 1564
SfcI CTRYAG 1 cut(s) 1569
SinI GGWCC 4 cut(s) 348, 1243, 1661, 1680
SmiMI CAYNNNNRTG 5 cut(s) 405, 619, 945, 1149, 1703
SphI GCATGC 1 cut(s) 1690
SseBI AGGCCT 2 cut(s) 337, 1504
SsiI CCGC 2 cut(s) 150, 520
SspI AATATT 2 cut(s) 1168, 1438
SspMI CTAG 2 cut(s) 1106, 1532
StuI AGGCCT 2 cut(s) 337, 1504
StyD4I CCNGG 4 cut(s) 376, 475, 1239, 1663
StyI CCWWGG 5 cut(s) 715, 1015, 1524, 1559, 1626
TaaI ACNGT 1 cut(s) 645
TaiI ACGT 1 cut(s) 1290
TaqI TCGA 3 cut(s) 30, 729, 1147
TatI WGTACW 3 cut(s) 84, 666, 1185
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 2 cut(s) 118, 229
Tru1I TTAA 4 cut(s) 66, 141, 1322, 1397
Tru9I TTAA 4 cut(s) 66, 141, 1322, 1397
TscAI CASTG 4 cut(s) 108, 226, 240, 1681
TseFI GTSAC 2 cut(s) 497, 1283
TseI GCWGC 3 cut(s) 1085, 1094, 1160
Tsp45I GTSAC 2 cut(s) 497, 1283
TspGWI ACGGA 2 cut(s) 715, 1161
TspRI CASTG 4 cut(s) 108, 226, 240, 1681
VpaK11BI GGWCC 4 cut(s) 348, 1243, 1661, 1680
XapI RAATTY 5 cut(s) 469, 534, 609, 741, 1352
XceI RCATGY 2 cut(s) 1690, 1702
XcmI CCANNNNNNNNNTGG 1 cut(s) 374
XmiI GTMKAC 4 cut(s) 729, 825, 835, 999
XmnI GAANNNNTTC 3 cut(s) 228, 299, 759
XspI CTAG 2 cut(s) 1106, 1532
ZrmI AGTACT 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.