MD05G1270900.v1.1

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000066
Physical Location & Seq
Forward (+)
40667368 .. 40669437
2070 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1270900.v1.1.491

Sequence Viewer

Length: 1521 bp
ATGACCTTCTGCGAGAGCTTAGGGAGCATTAGTGAGTCTTGCTCCGGCATTGGGTGCTGCCAGACTTCAATCCCAAGTGGGCTTCAAGTTCGTACCGTGATGATGAGTAGCTACTCCAATCATACGCTTATATGGGACTTTAATCCATGTAGCTATTCCTTCATTGTGGAAGAAGGCCAGTTCACATTTTCTAATAGAAGTTTCCAAGAGCTCGAAAACACAAGCCGGCTTCCGATGGTTCTCAATTGGGCAATTGGGGATGAAGAATGTGATGCAGCTCAAAAGAGGGAGGACTATGCATGCAAGGGAAATAGCACTTGTGTCAGCCCCGTCAATCTGTCCGGCTACTTTTGTCAGTGCTTGCCAGGTTACGAAGGAAATCCGTACCCCCCAGAGGGTTGTCATGCTGTGACAGTAGCCTCCTTGATTTTCCTGGTGGGAGGTTCATGGACCTACTGGGGAATGAAGAAAAACAACTACATCAAACTCAGAGAAAAGTATTTCAAAGAGAATGGTGGCATGTTGTTACAACAACGACCCGCGAGTCGCCAAGGAGGATTGGTGGAGACAACAAAAATCTTTACAGCAGAAGAACTTGACAAGGCTACAGGCTACTACGATGACAGTAGAGTCCTCGGCGAAGGAGGCTACGGTACAGTTTACAAAGGAATACTACCAGATAATGAAGTGGTTGCCATAAAAAAGTCGAAAATCGGAGCTTCAATCCAAAGTGAGCAGTTTATAAACGAAGTTATTGTTCTTTCTCAAGTCAACCACAGAAATGTGGTGAAGCTACTGGGTTGCTGTTTGGAGACACAAGTGCCTTTACTAGTTTACGAGTTCATCACCAGGGGAACTCTTTTTTATCATCTTCATAACGTAAAGAGCAAAGAATCGTCGCTTTCGTGGGAGTTACGACTGAAGATCGCAGCTGAAACTGCCGGAGCACTAGCGTACTTGCACTCCTCCACTTCCATGCCAATCATACACCGAGATCAAAAAGTGTCGGACTTCGGAGCTTCGCGATTGATTCCTCTAGATCAAGCTCAAATCACAACTCTAGTGCAAGGGACGCTAGGGTACTTGGACCCTGAATACTTCCATTCAAACCAGCTGACAGAAAAGAGTGACGTCTACAGTTTCGGAGTTGTTCTTGTGGAGCTGCTAACAAGAAAAGTGGCACTGTCATTTACTAGGCCTGAAGAAGAGAGAAACCCGGCAAACTTCTTCGTTTGTTCCGTGGAACAGGGTCGCTTGACTCAAATTCTCGATGACGACATAGTCACCGACAGAAATCTTGAGACACTGAAAAGAGTGGCTAATCTTGCAAGTAGATGCTTGAGGGTGAAAAGGGAGGAAAGGCCTAGCATGAAAAAGGTAGCAATGGAGCTAGAAGGATACAGACTCTGGCGAAGCATCCACGGAGAACTGCTGATTTCAGTCCAGAGGACACTGAATACTTGCTCGGGCCGCCTTCAATGGCTTATGCTAGAGGTGATAGTTGTTCCAGTACTAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

507

Amino Acids

56.78

Weight (kDa)

6.07

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 206 - 460 5.2e-35 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 210 - 464 6.7e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 743
AasI GACNNNNNNGTC 3 cut(s) 543, 629, 1280
AatII GACGTC 1 cut(s) 1134
AccI GTMKAC 1 cut(s) 1134
AccII CGCG 2 cut(s) 542, 1024
AciI CCGC 2 cut(s) 540, 1471
AcsI RAATTY 1 cut(s) 1263
AcuI CTGAAG 2 cut(s) 941, 1221
AcyI GRCGYC 1 cut(s) 1131
AfaI GTAC 6 cut(s) 94, 386, 655, 956, 1082, 1512
AfiI CCNNNNNNNGG 3 cut(s) 51, 394, 395
AgsI TTSAA 6 cut(s) 69, 86, 505, 723, 1107, 1478
AhlI ACTAGT 1 cut(s) 829
AjnI CCWGG 3 cut(s) 364, 432, 848
Alw21I GWGCWC 2 cut(s) 213, 949
Alw26I GTCTC 3 cut(s) 560, 806, 1295
AlwNI CAGNNNCTG 1 cut(s) 1407
Ama87I CYCGRG 1 cut(s) 1465
AoxI GGCC 4 cut(s) 175, 1196, 1361, 1468
ApeKI GCWGC 4 cut(s) 57, 275, 929, 1162
ApoI RAATTY 1 cut(s) 1263
AspS9I GGNCC 3 cut(s) 450, 1087, 1468
AsuC2I CCSGG 1 cut(s) 1217
AsuHPI GGTGA 5 cut(s) 799, 838, 1276, 1357, 1507
AvaI CYCGRG 1 cut(s) 1465
AvaII GGWCC 2 cut(s) 450, 1087
BanII GRGCYC 1 cut(s) 213
Bbv12I GWGCWC 2 cut(s) 213, 949
BbvI GCAGC 4 cut(s) 44, 287, 941, 1149
BccI CCATC 1 cut(s) 229
BciT130I CCWGG 3 cut(s) 366, 434, 850
BciVI GTATCC 1 cut(s) 1391
BcnI CCSGG 1 cut(s) 1217
BcoDI GTCTC 3 cut(s) 560, 806, 1295
BcuI ACTAGT 1 cut(s) 829
BfmI CTRYAG 2 cut(s) 606, 1135
BfuI GTATCC 1 cut(s) 1391
BisI GCNGC 5 cut(s) 58, 276, 930, 1163, 1471
BlsI GCNGC 5 cut(s) 59, 277, 931, 1164, 1472
BmcAI AGTACT 1 cut(s) 1512
Bme1390I CCNGG 4 cut(s) 366, 434, 850, 1217
Bme18I GGWCC 2 cut(s) 450, 1087
BmeT110I CYCGRG 1 cut(s) 1465
BmgT120I GGNCC 3 cut(s) 450, 1087, 1468
BmiI GGNNCC 1 cut(s) 1089
BmrFI CCNGG 4 cut(s) 366, 434, 850, 1217
BmrI ACTGGG 2 cut(s) 466, 806
BmsI GCATC 3 cut(s) 262, 1325, 1425
BmuI ACTGGG 2 cut(s) 466, 806
BplI GAGNNNNNCTC 2 cut(s) 26, 58
Bpu10I CCTNAGC 1 cut(s) 19
BpuEI CTTGAG 3 cut(s) 750, 1319, 1360
BpuMI CCSGG 1 cut(s) 1217
BsaHI GRCGYC 1 cut(s) 1131
BsaJI CCNNGG 5 cut(s) 550, 634, 849, 1239, 1420
BsaXI ACNNNNNCTCC 6 cut(s) 16, 46, 546, 576, 947, 977
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 394, 395
Bse118I RCCGGY 1 cut(s) 225
Bse1I ACTGG 4 cut(s) 178, 461, 801, 1508
Bse3DI GCAATG 1 cut(s) 1389
BseBI CCWGG 3 cut(s) 366, 434, 850
BseDI CCNNGG 5 cut(s) 550, 634, 849, 1239, 1420
BseGI GGATG 2 cut(s) 265, 1416
BseLI CCNNNNNNNGG 3 cut(s) 51, 394, 395
BseMI GCAATG 1 cut(s) 1389
BseMII CTCAG 1 cut(s) 502
BseNI ACTGG 4 cut(s) 178, 461, 801, 1508
BseRI GAGGAG 1 cut(s) 955
BseXI GCAGC 4 cut(s) 44, 287, 941, 1149
Bsh1236I CGCG 2 cut(s) 542, 1024
BshFI GGCC 4 cut(s) 177, 1198, 1363, 1470
BsiHKAI GWGCWC 2 cut(s) 213, 949
BsiHKCI CYCGRG 1 cut(s) 1465
BsiSI CCGG 5 cut(s) 45, 226, 342, 942, 1217
BslFI GGGAC 2 cut(s) 149, 1084
BslI CCNNNNNNNGG 3 cut(s) 51, 394, 395
BsmAI GTCTC 3 cut(s) 560, 806, 1295
BsmFI GGGAC 2 cut(s) 149, 1084
BsnI GGCC 4 cut(s) 177, 1198, 1363, 1470
BsoBI CYCGRG 1 cut(s) 1465
Bsp1286I GDGCHC 2 cut(s) 213, 949
Bsp143I GATC 3 cut(s) 924, 994, 1039
Bsp68I TCGCGA 1 cut(s) 1024
BspACI CCGC 2 cut(s) 540, 1471
BspANI GGCC 4 cut(s) 177, 1198, 1363, 1470
BspCNI CTCAG 1 cut(s) 501
BspFNI CGCG 2 cut(s) 542, 1024
BspLI GGNNCC 1 cut(s) 1089
BsrDI GCAATG 1 cut(s) 1389
BsrFI RCCGGY 1 cut(s) 225
BsrI ACTGG 4 cut(s) 178, 461, 801, 1508
BssAI RCCGGY 1 cut(s) 225
BssECI CCNNGG 5 cut(s) 550, 634, 849, 1239, 1420
BssMI GATC 3 cut(s) 924, 994, 1039
BssNI GRCGYC 1 cut(s) 1131
BssT1I CCWWGG 1 cut(s) 550
Bst2UI CCWGG 3 cut(s) 366, 434, 850
Bst4CI ACNGT 7 cut(s) 97, 415, 626, 653, 658, 1139, 1185
Bst6I CTCTTC 1 cut(s) 1200
BstACI GRCGYC 1 cut(s) 1131
BstAPI GCANNNNNTGC 1 cut(s) 54
BstC8I GCNNGC 3 cut(s) 227, 301, 362
BstDEI CTNAG 2 cut(s) 19, 488
BstDSI CCRYGG 2 cut(s) 1239, 1420
BstF5I GGATG 2 cut(s) 265, 1416
BstFNI CGCG 2 cut(s) 542, 1024
BstKTI GATC 3 cut(s) 927, 997, 1042
BstMAI GTCTC 3 cut(s) 560, 806, 1295
BstMBI GATC 3 cut(s) 924, 994, 1039
BstMWI GCNNNNNNNGC 7 cut(s) 24, 54, 645, 938, 1072, 1325, 1470
BstNI CCWGG 3 cut(s) 366, 434, 850
BstNSI RCATGY 2 cut(s) 303, 523
BstSCI CCNGG 4 cut(s) 364, 432, 848, 1215
BstSFI CTRYAG 2 cut(s) 606, 1135
BstUI CGCG 2 cut(s) 542, 1024
BstV1I GCAGC 4 cut(s) 44, 287, 941, 1149
BsuI GTATCC 1 cut(s) 1391
BsuRI GGCC 4 cut(s) 177, 1198, 1363, 1470
BtgI CCRYGG 2 cut(s) 1239, 1420
BtsCI GGATG 2 cut(s) 265, 1416
BtsIMutI CAGTG 4 cut(s) 362, 1181, 1304, 1451
BtuMI TCGCGA 1 cut(s) 1024
Cac8I GCNNGC 3 cut(s) 227, 301, 362
CaiI CAGNNNCTG 1 cut(s) 1407
Cfr10I RCCGGY 1 cut(s) 225
Cfr13I GGNCC 3 cut(s) 450, 1087, 1468
CseI GACGC 1 cut(s) 1081
Csp6I GTAC 6 cut(s) 93, 385, 654, 955, 1081, 1511
CspCI CAANNNNNGTGG 2 cut(s) 1158, 1193
CviAII CATG 7 cut(s) 147, 300, 404, 447, 520, 976, 1369
CviQI GTAC 6 cut(s) 93, 385, 654, 955, 1081, 1511
DdeI CTNAG 2 cut(s) 19, 488
DpnI GATC 3 cut(s) 926, 996, 1041
DpnII GATC 3 cut(s) 924, 994, 1039
DrdI GACNNNNNNGTC 3 cut(s) 543, 629, 1280
DseDI GACNNNNNNGTC 3 cut(s) 543, 629, 1280
Eam1104I CTCTTC 1 cut(s) 1200
EarI CTCTTC 1 cut(s) 1200
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 550
Eco147I AGGCCT 2 cut(s) 1198, 1363
Eco24I GRGCYC 1 cut(s) 213
Eco47I GGWCC 2 cut(s) 450, 1087
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 2 cut(s) 941, 1221
Eco88I CYCGRG 1 cut(s) 1465
EcoICRI GAGCTC 1 cut(s) 211
EcoRII CCWGG 3 cut(s) 364, 432, 848
EcoT14I CCWWGG 1 cut(s) 550
EcoT22I ATGCAT 1 cut(s) 301
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 550
FaeI CATG 7 cut(s) 150, 303, 407, 450, 523, 979, 1372
FaqI GGGAC 2 cut(s) 149, 1084
FatI CATG 7 cut(s) 146, 299, 403, 446, 519, 975, 1368
FauI CCCGC 1 cut(s) 547
FblI GTMKAC 1 cut(s) 1134
Fnu4HI GCNGC 5 cut(s) 58, 276, 930, 1163, 1471
FokI GGATG 2 cut(s) 272, 1403
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 5 cut(s) 58, 276, 930, 1163, 1471
GluI GCNGC 5 cut(s) 58, 276, 930, 1163, 1471
HaeIII GGCC 4 cut(s) 177, 1198, 1363, 1470
HapII CCGG 5 cut(s) 45, 226, 342, 942, 1217
HgaI GACGC 1 cut(s) 1081
Hin1I GRCGYC 1 cut(s) 1131
Hin1II CATG 7 cut(s) 150, 303, 407, 450, 523, 979, 1372
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HinfI GANTC 7 cut(s) 35, 544, 630, 893, 1030, 1258, 1404
HpaII CCGG 5 cut(s) 45, 226, 342, 942, 1217
HphI GGTGA 5 cut(s) 799, 838, 1276, 1357, 1507
Hpy166II GTNNAC 5 cut(s) 183, 661, 772, 835, 1135
Hpy188I TCNGA 6 cut(s) 234, 491, 716, 1009, 1016, 1145
Hpy188III TCNNGA 5 cut(s) 1023, 1037, 1268, 1298, 1444
Hpy8I GTNNAC 5 cut(s) 183, 661, 772, 835, 1135
Hpy99I CGWCG 1 cut(s) 901
HpyAV CCTTC 7 cut(s) 16, 167, 169, 368, 635, 1388, 1484
HpyCH4III ACNGT 7 cut(s) 97, 415, 626, 653, 658, 1139, 1185
HpyCH4IV ACGT 2 cut(s) 879, 1131
HpyCH4V TGCA 6 cut(s) 275, 299, 303, 961, 1066, 1328
HpyF10VI GCNNNNNNNGC 7 cut(s) 24, 54, 645, 938, 1072, 1325, 1470
HpyF3I CTNAG 2 cut(s) 19, 488
HpySE526I ACGT 2 cut(s) 879, 1131
Hsp92I GRCGYC 1 cut(s) 1131
Hsp92II CATG 7 cut(s) 150, 303, 407, 450, 523, 979, 1372
KroI GCCGGC 1 cut(s) 225
KroNI GCCGGC 1 cut(s) 227
Kzo9I GATC 3 cut(s) 924, 994, 1039
LmnI GCTCC 7 cut(s) 24, 47, 716, 944, 1016, 1159, 1387
Lsp1109I GCAGC 4 cut(s) 44, 287, 941, 1149
LweI GCATC 3 cut(s) 262, 1325, 1425
MaeII ACGT 2 cut(s) 879, 1131
MaeIII GTNAC 6 cut(s) 368, 409, 525, 912, 1127, 1282
MalI GATC 3 cut(s) 926, 996, 1041
MboI GATC 3 cut(s) 924, 994, 1039
MboII GAAGA 9 cut(s) 182, 275, 478, 602, 863, 934, 1214, 1217, 1219
MfeI CAATTG 2 cut(s) 244, 252
MhlI GDGCHC 2 cut(s) 213, 949
MluCI AATT 3 cut(s) 244, 252, 1263
MlyI GAGTC 5 cut(s) 44, 553, 639, 1252, 1398
MmeI TCCRAC 1 cut(s) 987
Mph1103I ATGCAT 1 cut(s) 301
MroNI GCCGGC 1 cut(s) 225
MseI TTAA 1 cut(s) 141
MslI CAYNNNNRTG 2 cut(s) 780, 974
MspA1I CMGCKG 2 cut(s) 932, 1114
MspI CCGG 5 cut(s) 45, 226, 342, 942, 1217
MspR9I CCNGG 4 cut(s) 366, 434, 850, 1217
MunI CAATTG 2 cut(s) 244, 252
MvaI CCWGG 3 cut(s) 366, 434, 850
MvnI CGCG 2 cut(s) 542, 1024
MwoI GCNNNNNNNGC 7 cut(s) 24, 54, 645, 938, 1072, 1325, 1470
NaeI GCCGGC 1 cut(s) 227
NciI CCSGG 1 cut(s) 1217
NdeII GATC 3 cut(s) 924, 994, 1039
NgoMIV GCCGGC 1 cut(s) 225
NlaIII CATG 7 cut(s) 150, 303, 407, 450, 523, 979, 1372
NlaIV GGNNCC 1 cut(s) 1089
NmeAIII GCCGAG 1 cut(s) 615
NmuCI GTSAC 3 cut(s) 409, 1127, 1282
NruI TCGCGA 1 cut(s) 1024
NsiI ATGCAT 1 cut(s) 301
NspI RCATGY 2 cut(s) 303, 523
PaeI GCATGC 1 cut(s) 303
PceI AGGCCT 2 cut(s) 1198, 1363
PcsI WCGNNNNNNNCGW 2 cut(s) 902, 1236
PdiI GCCGGC 1 cut(s) 227
PfeI GAWTC 2 cut(s) 893, 1030
PflFI GACNNNGTC 1 cut(s) 1280
PkrI GCNGC 5 cut(s) 59, 277, 931, 1164, 1472
PleI GAGTC 5 cut(s) 43, 552, 638, 1252, 1398
PpsI GAGTC 5 cut(s) 43, 552, 638, 1252, 1398
PsiI TTATAA 1 cut(s) 743
Psp124BI GAGCTC 1 cut(s) 213
Psp6I CCWGG 3 cut(s) 364, 432, 848
PspGI CCWGG 3 cut(s) 364, 432, 848
PspN4I GGNNCC 1 cut(s) 1089
PspPI GGNCC 3 cut(s) 450, 1087, 1468
PstNI CAGNNNCTG 1 cut(s) 1407
PsyI GACNNNGTC 1 cut(s) 1280
PvuII CAGCTG 2 cut(s) 932, 1114
RruI TCGCGA 1 cut(s) 1024
RsaI GTAC 6 cut(s) 94, 386, 655, 956, 1082, 1512
RsaNI GTAC 6 cut(s) 93, 385, 654, 955, 1081, 1511
RseI CAYNNNNRTG 2 cut(s) 780, 974
SacI GAGCTC 1 cut(s) 213
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 5 cut(s) 58, 276, 930, 1163, 1471
Sau3AI GATC 3 cut(s) 924, 994, 1039
Sau96I GGNCC 3 cut(s) 450, 1087, 1468
ScaI AGTACT 1 cut(s) 1512
SchI GAGTC 5 cut(s) 44, 553, 639, 1252, 1398
ScrFI CCNGG 4 cut(s) 366, 434, 850, 1217
SduI GDGCHC 2 cut(s) 213, 949
SfaNI GCATC 3 cut(s) 262, 1325, 1425
SfcI CTRYAG 2 cut(s) 606, 1135
SinI GGWCC 2 cut(s) 450, 1087
SmiMI CAYNNNNRTG 2 cut(s) 780, 974
SmlI CTYRAG 3 cut(s) 765, 1298, 1339
SmoI CTYRAG 3 cut(s) 765, 1298, 1339
SpeI ACTAGT 1 cut(s) 829
SphI GCATGC 1 cut(s) 303
Sse9I AATT 3 cut(s) 244, 252, 1263
SseBI AGGCCT 2 cut(s) 1198, 1363
SsiI CCGC 2 cut(s) 540, 1471
SstI GAGCTC 1 cut(s) 213
StuI AGGCCT 2 cut(s) 1198, 1363
StyD4I CCNGG 4 cut(s) 364, 432, 848, 1215
StyI CCWWGG 1 cut(s) 550
TaaI ACNGT 7 cut(s) 97, 415, 626, 653, 658, 1139, 1185
TaiI ACGT 2 cut(s) 882, 1134
TaqI TCGA 3 cut(s) 213, 707, 1269
TasI AATT 3 cut(s) 244, 252, 1263
TatI WGTACW 1 cut(s) 1510
TauI GCSGC 1 cut(s) 1473
TfiI GAWTC 2 cut(s) 893, 1030
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 4 cut(s) 362, 1188, 1311, 1458
TseFI GTSAC 3 cut(s) 409, 1127, 1282
TseI GCWGC 4 cut(s) 57, 275, 929, 1162
Tsp45I GTSAC 3 cut(s) 409, 1127, 1282
TspDTI ATGAA 8 cut(s) 151, 276, 435, 479, 699, 832, 863, 1385
TspGWI ACGGA 3 cut(s) 372, 1228, 1437
TspRI CASTG 4 cut(s) 362, 1188, 1311, 1458
Tth111I GACNNNGTC 1 cut(s) 1280
VpaK11BI GGWCC 2 cut(s) 450, 1087
XapI RAATTY 1 cut(s) 1263
XbaI TCTAGA 1 cut(s) 1036
XceI RCATGY 2 cut(s) 303, 523
XmiI GTMKAC 1 cut(s) 1134
ZraI GACGTC 1 cut(s) 1132
ZrmI AGTACT 1 cut(s) 1512
Zsp2I ATGCAT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.