Rroxscaffold_3G00236220

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000066
Physical Location & Seq
Reverse (-)
23386783 .. 23389721
2939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00236220.1

Sequence Viewer

Length: 2130 bp
ATGCAATTCATACTGGTAGCGGTGCTGGTTCTCTTCTCATTTGCGGCCACGGCTGAAGAAGCAGTAGCAGCTGAAGCCGTCCTGCCTCAAGCCAAGCCTGACTGCACTGACCGCTGTGGCAACCTCACCATTCCATATCCATTTGGCATAGGGCCTAATTGTTACATGGCAGAGGCCTTTTCTGTTAGCTGTAACACATCCACTAAACCCCCGACAGCAATGTGGCGTAATAGTCCCATCGCTATTAACAACATATACCTGGCTGAAGGTGAGATGCAAATAACGCAGTACGTAGCCTACGATTGCTATAACAGCCAGGGCTCGACTGATTACAACCAACCAATTCTCGCCGTGCCTCCTTTCACCGTCTCTCATACCAGAAACAAGTTCATTGCCGTTGGTTGCGATACTTATGCACTTTTCAGCGGTTACCGTGCAGACGAAGAGAGGTACATGACCGGATGCATGTCCATATGTGACAACCTTGACAGCGTTGAACAGTCTTGCTCCGGGGTTGGGTGTTGCCTAACTGACATCCCTAGAGGACTTGAAAACTACACTGTCAGACTGGATAGCTACTATAATCATACATACATACTGGACTTCAATCCATGCAGCTATGCCTTCATTGTGGAAGAAGGCAAGTTCACCTTTTCTCCGAATACAAGTTTTAACGACCTCTACAACACTGAAGAGCTTCCCATGATTCTTAATTGGGAAATCGGGAAAGGGCCATGTGATGAAGCTCAAAAGAAAGATGATTATGCATGCAAGGAAAATAGTAAGTGTGTCAATCGGACCATCAGCGATGAGCTATCTGGTTATATTTGTCACTGCCAGCCGGGTTATCAAGGGAACCCCTACCTCCCTGATGGTTGCCAAGATATTGACGAGTGCAAGGTTTCAAAACCATGCACCAATGGAAGGTGTGAAAATTCACCTGGAAGTTACTCTTGTTTGTGTGACAAAGGGTACAGAAGCAATGGAACCAATAATCAAAGTTGTATCAAGGAGGTTCCCAAAAATCAACCAAAGACCACGCTCCTGCTCCTGCTTATTAAACTCGCTAATCATGGAGGCTCAGTGGAGACAACAAAAATATTCACCACAGAAGAACTAGAGAAGGCAACAAACAATTACCATGAGAGTAGAGTCCTTGGAGAAGGAGGCTACGGAACAGTTTATAAAGGGGTACTACTTGACAACAAAGTGGTTGCCATAAAGAAGTCCAAAGGTGGCACCTCGATCCAGAGTGAACAGTTTGTTAACGAGGTCGTTGTTCTTTCCCAAATCAACCACAGAAATGTGGTGAAGCTACTAGGTTGTTGTTTAGAGACATCAGTACCTTTACTAGTATATGAGTTTGTCACCCATGGCACTTTATATGAGCACATTCATAGAAAAAGATCACTACTGTCGTTCGAACTACGAATGAAGATAGCTGTTGAAACAGCAGGAGCACTAGCATACTTACACTCCTCTATATCCACGCCAATCATACATCGAGATGTGAAAGCAGCAAATATACTCCTAGATGATAATTATACAGCTAAAGTGTCGGACTTTGGAGCTTCACGGTTGGTTCCTTCAGGTCAAACTGAAATACAAACTTTAGTGCTCGGGACATTTGGATACCTAGACCCAGAATATTTGCAGTCAAACCAACTAACAGAAAAAAGTGATGTCTACAGCTTTGGAGTTGTCCTAGTGGAGCTACTAACAAGCAAAGTGGCATTTTCTAATGATAGATGCTTAGCAAGCATCTTTGTTTCTTCCATGGATGAAGATTGCTTAAAACAAATTCTTGATGATGCCATAGTGAACGAGGGAAACATTGAGACAGTAAAAAACGTGGCGAGTCTCGCAAAAAGATGTTTGAGGGTAAAAGGGGAGGAAAGGCCTACCATGAAAGAAGTTGCCCTGGAGTTAGAGGGAATGACAATTATGGCAAAGCATCCATGGGGAAAGAACGCCATGTTTTGTCCAGAGGAGAATGAGTACTTGCTTGGGTCACCTTCAGACAGTTATGTTATGGATGTTAGAGGAGGTGCTGGCAATTCTAGTGGTTTAACTACTGGGTATGACAGCATGCAAATCCAGATGTTGATGCCACGTGGTGATGGACGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

709

Amino Acids

78.26

Weight (kDa)

5.05

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 95 5.3e-10 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 295 - 335 8.8e-08 Calcium-binding EGF domain
Pkinase PF00069 380 - 639 3.8e-51 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 383 - 642 7.2e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1185
AccB1I GGYRCC 1 cut(s) 1238
AccI GTMKAC 1 cut(s) 1686
AciI CCGC 4 cut(s) 20, 44, 112, 426
AclWI GGATC 1 cut(s) 1240
AcoI YGGCCR 1 cut(s) 45
AcsI RAATTY 2 cut(s) 934, 1800
AcuI CTGAAG 6 cut(s) 75, 93, 285, 711, 1572, 2001
AcvI CACGTG 1 cut(s) 2114
AdeI CACNNNGTG 1 cut(s) 2117
AfaI GTAC 6 cut(s) 290, 452, 974, 1194, 1344, 2000
AfiI CCNNNNNNNGG 2 cut(s) 516, 924
AgsI TTSAA 5 cut(s) 497, 551, 607, 906, 1448
AhlI ACTAGT 1 cut(s) 1351
AjnI CCWGG 4 cut(s) 258, 315, 940, 1920
Alw21I GWGCWC 3 cut(s) 1392, 1462, 1620
Alw26I GTCTC 5 cut(s) 373, 1082, 1328, 1832, 1865
AlwI GGATC 1 cut(s) 1240
Ama87I CYCGRG 1 cut(s) 1619
AoxI GGCC 5 cut(s) 45, 152, 174, 731, 1898
ApeKI GCWGC 3 cut(s) 68, 615, 1517
ApoI RAATTY 2 cut(s) 934, 1800
Asp700I GAANNNNTTC 1 cut(s) 696
AspS9I GGNCC 3 cut(s) 152, 731, 798
AsuC2I CCSGG 2 cut(s) 511, 843
AsuII TTCGAA 1 cut(s) 1422
AvaI CYCGRG 1 cut(s) 1619
AvaII GGWCC 1 cut(s) 798
BaeI ACNNNNGTAYC 2 cut(s) 1326, 1359
BanI GGYRCC 1 cut(s) 1238
BanII GRGCYC 1 cut(s) 323
BbrPI CACGTG 1 cut(s) 2114
Bbv12I GWGCWC 3 cut(s) 1392, 1462, 1620
BbvI GCAGC 3 cut(s) 80, 627, 1529
BccI CCATC 4 cut(s) 245, 809, 866, 2114
BceAI ACGGC 4 cut(s) 62, 66, 335, 380
BcgI CGANNNNNNTGC 2 cut(s) 395, 429
BciT130I CCWGG 4 cut(s) 260, 317, 942, 1922
BciVI GTATCC 1 cut(s) 1625
BcnI CCSGG 2 cut(s) 511, 843
BcoDI GTCTC 5 cut(s) 373, 1082, 1328, 1832, 1865
BcuI ACTAGT 1 cut(s) 1351
BfaI CTAG 9 cut(s) 540, 1118, 1319, 1352, 1463, 1532, 1637, 1706, 2061
BfmI CTRYAG 1 cut(s) 1687
BfuI GTATCC 1 cut(s) 1625
BisI GCNGC 4 cut(s) 45, 69, 616, 1518
BlpI GCTNAGC 1 cut(s) 1753
BlsI GCNGC 4 cut(s) 46, 70, 617, 1519
BmcAI AGTACT 1 cut(s) 2000
Bme1390I CCNGG 6 cut(s) 260, 317, 511, 843, 942, 1922
Bme18I GGWCC 1 cut(s) 798
BmeT110I CYCGRG 1 cut(s) 1619
BmgT120I GGNCC 3 cut(s) 152, 731, 798
BmiI GGNNCC 5 cut(s) 857, 988, 1017, 1240, 1584
BmrFI CCNGG 6 cut(s) 260, 317, 511, 843, 942, 1922
BmrI ACTGGG 1 cut(s) 2085
BmsI GCATC 7 cut(s) 264, 452, 1739, 1770, 1801, 1963, 2097
BmuI ACTGGG 1 cut(s) 2085
BpmI CTGGAG 1 cut(s) 1943
Bpu1102I GCTNAGC 1 cut(s) 1753
Bpu14I TTCGAA 1 cut(s) 1422
BpuEI CTTGAG 1 cut(s) 72
BpuMI CCSGG 2 cut(s) 511, 843
BsaAI YACGTR 2 cut(s) 292, 2114
BsaJI CCNNGG 8 cut(s) 48, 316, 510, 1156, 1372, 1776, 1920, 1958
BsaWI WCCGGW 1 cut(s) 458
BsaXI ACNNNNNCTCC 8 cut(s) 640, 670, 1068, 1098, 1460, 1490, 1982, 2012
Bsc4I CCNNNNNNNGG 2 cut(s) 516, 924
Bse1I ACTGG 4 cut(s) 18, 573, 603, 2080
Bse3DI GCAATG 3 cut(s) 225, 390, 988
BseBI CCWGG 4 cut(s) 260, 317, 942, 1922
BseDI CCNNGG 8 cut(s) 48, 316, 510, 1156, 1372, 1776, 1920, 1958
BseGI GGATG 6 cut(s) 197, 467, 534, 1786, 1954, 2041
BseLI CCNNNNNNNGG 2 cut(s) 516, 924
BseMI GCAATG 3 cut(s) 225, 390, 988
BseMII CTCAG 1 cut(s) 1095
BseNI ACTGG 4 cut(s) 18, 573, 603, 2080
BseRI GAGGAG 3 cut(s) 1468, 2003, 2058
BseXI GCAGC 3 cut(s) 80, 627, 1529
BsgI GTGCAG 2 cut(s) 88, 456
BshFI GGCC 5 cut(s) 47, 154, 176, 733, 1900
BshNI GGYRCC 1 cut(s) 1238
BsiHKAI GWGCWC 3 cut(s) 1392, 1462, 1620
BsiHKCI CYCGRG 1 cut(s) 1619
BsiSI CCGG 3 cut(s) 459, 510, 842
BslFI GGGAC 2 cut(s) 219, 1636
BslI CCNNNNNNNGG 2 cut(s) 516, 924
BsmAI GTCTC 5 cut(s) 373, 1082, 1328, 1832, 1865
BsmBI CGTCTC 1 cut(s) 373
BsmFI GGGAC 2 cut(s) 219, 1636
BsnI GGCC 5 cut(s) 47, 154, 176, 733, 1900
BsoBI CYCGRG 1 cut(s) 1619
Bsp119I TTCGAA 1 cut(s) 1422
Bsp1286I GDGCHC 4 cut(s) 323, 1392, 1462, 1620
Bsp143I GATC 2 cut(s) 1245, 1406
Bsp1720I GCTNAGC 1 cut(s) 1753
Bsp19I CCATGG 3 cut(s) 1372, 1776, 1958
BspACI CCGC 4 cut(s) 20, 44, 112, 426
BspANI GGCC 5 cut(s) 47, 154, 176, 733, 1900
BspCNI CTCAG 1 cut(s) 1094
BspLI GGNNCC 5 cut(s) 857, 988, 1017, 1240, 1584
BspPI GGATC 1 cut(s) 1240
BspQI GCTCTTC 1 cut(s) 687
BspT104I TTCGAA 1 cut(s) 1422
BspT107I GGYRCC 1 cut(s) 1238
BsrDI GCAATG 3 cut(s) 225, 390, 988
BsrI ACTGG 4 cut(s) 18, 573, 603, 2080
BssECI CCNNGG 8 cut(s) 48, 316, 510, 1156, 1372, 1776, 1920, 1958
BssMI GATC 2 cut(s) 1245, 1406
BssT1I CCWWGG 4 cut(s) 1156, 1372, 1776, 1958
Bst2UI CCWGG 4 cut(s) 260, 317, 942, 1922
Bst6I CTCTTC 3 cut(s) 38, 438, 687
BstBAI YACGTR 2 cut(s) 292, 2114
BstBI TTCGAA 1 cut(s) 1422
BstC8I GCNNGC 5 cut(s) 769, 839, 1759, 2053, 2090
BstDEI CTNAG 2 cut(s) 1081, 1753
BstDSI CCRYGG 4 cut(s) 48, 1372, 1776, 1958
BstEII GGTNACC 2 cut(s) 428, 2010
BstF5I GGATG 6 cut(s) 197, 467, 534, 1786, 1954, 2041
BstKTI GATC 2 cut(s) 1248, 1409
BstMAI GTCTC 5 cut(s) 373, 1082, 1328, 1832, 1865
BstMBI GATC 2 cut(s) 1245, 1406
BstMWI GCNNNNNNNGC 9 cut(s) 50, 59, 68, 74, 111, 283, 312, 1758, 1862
BstNI CCWGG 4 cut(s) 260, 317, 942, 1922
BstNSI RCATGY 3 cut(s) 469, 771, 2092
BstPI GGTNACC 2 cut(s) 428, 2010
BstSCI CCNGG 6 cut(s) 258, 315, 509, 841, 940, 1920
BstSFI CTRYAG 1 cut(s) 1687
BstSNI TACGTA 1 cut(s) 292
BstV1I GCAGC 3 cut(s) 80, 627, 1529
BsuI GTATCC 1 cut(s) 1625
BsuRI GGCC 5 cut(s) 47, 154, 176, 733, 1900
BtgI CCRYGG 4 cut(s) 48, 1372, 1776, 1958
BtgZI GCGATG 2 cut(s) 223, 822
BtsCI GGATG 6 cut(s) 197, 467, 534, 1786, 1954, 2041
BtsI GCAGTG 1 cut(s) 832
BtsIMutI CAGTG 5 cut(s) 105, 558, 687, 832, 1089
Cac8I GCNNGC 5 cut(s) 769, 839, 1759, 2053, 2090
Cfr13I GGNCC 3 cut(s) 152, 731, 798
Csp6I GTAC 6 cut(s) 289, 451, 973, 1193, 1343, 1999
CspCI CAANNNNNGTGG 4 cut(s) 1710, 1745, 2044, 2079
CviQI GTAC 6 cut(s) 289, 451, 973, 1193, 1343, 1999
DdeI CTNAG 2 cut(s) 1081, 1753
DpnI GATC 2 cut(s) 1247, 1408
DpnII GATC 2 cut(s) 1245, 1406
DraIII CACNNNGTG 1 cut(s) 2117
EaeI YGGCCR 1 cut(s) 45
Eam1104I CTCTTC 3 cut(s) 38, 438, 687
EarI CTCTTC 3 cut(s) 38, 438, 687
Eco105I TACGTA 1 cut(s) 292
Eco130I CCWWGG 4 cut(s) 1156, 1372, 1776, 1958
Eco147I AGGCCT 2 cut(s) 176, 1900
Eco24I GRGCYC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 798
Eco57I CTGAAG 6 cut(s) 75, 93, 285, 711, 1572, 2001
Eco72I CACGTG 1 cut(s) 2114
Eco88I CYCGRG 1 cut(s) 1619
Eco91I GGTNACC 2 cut(s) 428, 2010
EcoO109I RGGNCCY 1 cut(s) 152
EcoO65I GGTNACC 2 cut(s) 428, 2010
EcoRII CCWGG 4 cut(s) 258, 315, 940, 1920
EcoT14I CCWWGG 4 cut(s) 1156, 1372, 1776, 1958
EcoT22I ATGCAT 2 cut(s) 467, 769
EcoT38I GRGCYC 1 cut(s) 323
ErhI CCWWGG 4 cut(s) 1156, 1372, 1776, 1958
Esp3I CGTCTC 1 cut(s) 373
FalI AAGNNNNNCTT 4 cut(s) 635, 667, 937, 969
FaqI GGGAC 2 cut(s) 219, 1636
FauNDI CATATG 1 cut(s) 473
FblI GTMKAC 1 cut(s) 1686
Fnu4HI GCNGC 4 cut(s) 45, 69, 616, 1518
FokI GGATG 6 cut(s) 184, 474, 521, 1793, 1941, 2048
FriOI GRGCYC 1 cut(s) 323
Fsp4HI GCNGC 4 cut(s) 45, 69, 616, 1518
FspBI CTAG 9 cut(s) 540, 1118, 1319, 1352, 1463, 1532, 1637, 1706, 2061
GluI GCNGC 4 cut(s) 45, 69, 616, 1518
GsuI CTGGAG 1 cut(s) 1943
HaeIII GGCC 5 cut(s) 47, 154, 176, 733, 1900
HapII CCGG 3 cut(s) 459, 510, 842
HincII GTYRAC 1 cut(s) 1267
HindII GTYRAC 1 cut(s) 1267
HinfI GANTC 3 cut(s) 706, 1152, 1858
HpaI GTTAAC 1 cut(s) 1267
HpaII CCGG 3 cut(s) 459, 510, 842
Hpy166II GTNNAC 5 cut(s) 648, 1256, 1267, 1687, 1822
Hpy188I TCNGA 5 cut(s) 566, 660, 798, 1561, 2020
Hpy188III TCNNGA 7 cut(s) 724, 1249, 1505, 1621, 1805, 1985, 2098
Hpy8I GTNNAC 5 cut(s) 648, 1256, 1267, 1687, 1822
HpyAV CCTTC 8 cut(s) 260, 632, 634, 918, 1117, 1157, 1596, 2025
HpyCH4IV ACGT 3 cut(s) 291, 1851, 2113
HpyF10VI GCNNNNNNNGC 9 cut(s) 50, 59, 68, 74, 111, 283, 312, 1758, 1862
HpyF3I CTNAG 2 cut(s) 1081, 1753
HpySE526I ACGT 3 cut(s) 291, 1851, 2113
KspAI GTTAAC 1 cut(s) 1267
Kzo9I GATC 2 cut(s) 1245, 1406
LguI GCTCTTC 1 cut(s) 687
LmnI GCTCC 6 cut(s) 512, 1047, 1053, 1457, 1568, 1711
Lsp1109I GCAGC 3 cut(s) 80, 627, 1529
LweI GCATC 7 cut(s) 264, 452, 1739, 1770, 1801, 1963, 2097
MaeI CTAG 9 cut(s) 540, 1118, 1319, 1352, 1463, 1532, 1637, 1706, 2061
MaeII ACGT 3 cut(s) 291, 1851, 2113
MaeIII GTNAC 9 cut(s) 161, 191, 428, 476, 830, 947, 962, 1366, 2010
MalI GATC 2 cut(s) 1247, 1408
MboI GATC 2 cut(s) 1245, 1406
MboII GAAGA 9 cut(s) 25, 68, 455, 647, 704, 1124, 1447, 1764, 1796
MhlI GDGCHC 4 cut(s) 323, 1392, 1462, 1620
MlyI GAGTC 2 cut(s) 1161, 1867
MmeI TCCRAC 1 cut(s) 1539
Mph1103I ATGCAT 2 cut(s) 467, 769
MroXI GAANNNNTTC 1 cut(s) 696
MseI TTAA 7 cut(s) 246, 672, 711, 1059, 1266, 1793, 2069
MslI CAYNNNNRTG 2 cut(s) 1302, 1506
MspA1I CMGCKG 3 cut(s) 71, 114, 426
MspI CCGG 3 cut(s) 459, 510, 842
MspR9I CCNGG 6 cut(s) 260, 317, 511, 843, 942, 1922
MvaI CCWGG 4 cut(s) 260, 317, 942, 1922
MwoI GCNNNNNNNGC 9 cut(s) 50, 59, 68, 74, 111, 283, 312, 1758, 1862
NciI CCSGG 2 cut(s) 511, 843
NcoI CCATGG 3 cut(s) 1372, 1776, 1958
NdeI CATATG 1 cut(s) 473
NdeII GATC 2 cut(s) 1245, 1406
NlaIV GGNNCC 5 cut(s) 857, 988, 1017, 1240, 1584
NmuCI GTSAC 5 cut(s) 476, 830, 962, 1366, 2010
NsiI ATGCAT 2 cut(s) 467, 769
NspI RCATGY 3 cut(s) 469, 771, 2092
NspV TTCGAA 1 cut(s) 1422
PaeI GCATGC 2 cut(s) 771, 2092
PceI AGGCCT 2 cut(s) 176, 1900
PciSI GCTCTTC 1 cut(s) 687
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PdmI GAANNNNTTC 1 cut(s) 696
PfeI GAWTC 1 cut(s) 706
PkrI GCNGC 4 cut(s) 46, 70, 617, 1519
PleI GAGTC 2 cut(s) 1160, 1866
PmaCI CACGTG 1 cut(s) 2114
PmlI CACGTG 1 cut(s) 2114
PpsI GAGTC 2 cut(s) 1160, 1866
Ppu21I YACGTR 2 cut(s) 292, 2114
PsiI TTATAA 1 cut(s) 1185
Psp6I CCWGG 4 cut(s) 258, 315, 940, 1920
PspCI CACGTG 1 cut(s) 2114
PspEI GGTNACC 2 cut(s) 428, 2010
PspGI CCWGG 4 cut(s) 258, 315, 940, 1920
PspN4I GGNNCC 5 cut(s) 857, 988, 1017, 1240, 1584
PspPI GGNCC 3 cut(s) 152, 731, 798
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 6 cut(s) 290, 452, 974, 1194, 1344, 2000
RsaNI GTAC 6 cut(s) 289, 451, 973, 1193, 1343, 1999
RseI CAYNNNNRTG 2 cut(s) 1302, 1506
SapI GCTCTTC 1 cut(s) 687
SaqAI TTAA 7 cut(s) 246, 672, 711, 1059, 1266, 1793, 2069
SatI GCNGC 4 cut(s) 45, 69, 616, 1518
Sau3AI GATC 2 cut(s) 1245, 1406
Sau96I GGNCC 3 cut(s) 152, 731, 798
ScaI AGTACT 1 cut(s) 2000
SchI GAGTC 2 cut(s) 1161, 1867
ScrFI CCNGG 6 cut(s) 260, 317, 511, 843, 942, 1922
SduI GDGCHC 4 cut(s) 323, 1392, 1462, 1620
SfaNI GCATC 7 cut(s) 264, 452, 1739, 1770, 1801, 1963, 2097
SfcI CTRYAG 1 cut(s) 1687
SfuI TTCGAA 1 cut(s) 1422
SinI GGWCC 1 cut(s) 798
SmiMI CAYNNNNRTG 2 cut(s) 1302, 1506
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
SnaBI TACGTA 1 cut(s) 292
SpeI ACTAGT 1 cut(s) 1351
SphI GCATGC 2 cut(s) 771, 2092
SseBI AGGCCT 2 cut(s) 176, 1900
SsiI CCGC 4 cut(s) 20, 44, 112, 426
SspI AATATT 2 cut(s) 1101, 1649
SspMI CTAG 9 cut(s) 540, 1118, 1319, 1352, 1463, 1532, 1637, 1706, 2061
StuI AGGCCT 2 cut(s) 176, 1900
StyD4I CCNGG 6 cut(s) 258, 315, 509, 841, 940, 1920
StyI CCWWGG 4 cut(s) 1156, 1372, 1776, 1958
TaiI ACGT 3 cut(s) 294, 1854, 2116
TaqI TCGA 4 cut(s) 323, 1244, 1422, 1504
TatI WGTACW 1 cut(s) 1998
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 1 cut(s) 706
Tru1I TTAA 7 cut(s) 246, 672, 711, 1059, 1266, 1793, 2069
Tru9I TTAA 7 cut(s) 246, 672, 711, 1059, 1266, 1793, 2069
TscAI CASTG 5 cut(s) 112, 565, 694, 839, 1089
TseFI GTSAC 5 cut(s) 476, 830, 962, 1366, 2010
TseI GCWGC 3 cut(s) 68, 615, 1517
Tsp45I GTSAC 5 cut(s) 476, 830, 962, 1366, 2010
TspDTI ATGAA 7 cut(s) 379, 616, 756, 1385, 1448, 1797, 1922
TspGWI ACGGA 1 cut(s) 1188
TspRI CASTG 5 cut(s) 112, 565, 694, 839, 1089
VpaK11BI GGWCC 1 cut(s) 798
XapI RAATTY 2 cut(s) 934, 1800
XceI RCATGY 3 cut(s) 469, 771, 2092
XmiI GTMKAC 1 cut(s) 1686
XmnI GAANNNNTTC 1 cut(s) 696
XspI CTAG 9 cut(s) 540, 1118, 1319, 1352, 1463, 1532, 1637, 1706, 2061
ZrmI AGTACT 1 cut(s) 2000
Zsp2I ATGCAT 2 cut(s) 467, 769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.