FvH4_2g35051

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000066
Physical Location & Seq
Forward (+)
25917776 .. 25920936
3161 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35051.t1

Sequence Viewer

Length: 2256 bp
ATGAGCAGAGACAAAGTAGTTTTGCTGCCGCAATTGGTGCTGGTTGCAATAGAGGTGCTACTAGTATCATCCCAAACAGTGGCTAGAGAGGGTTGCCAAGACCAATGTGGCAGTGTTAGCATTCCCTATCCGTTCGGAACAAAAGGGGGTTGTTACTATAATGATGATTTCCTCATTACTTGTGATGACACTCATTATGACCCTCCAAAGCCATTTCTTCGAGTGAGTGACATTGAGGTCACAAACATATCCATTGATGGGAAGCTCTACATTACTCAAAAGAAAGCCATGGCTTGTTTTTACGAATCAAGTAAGCCGTCGGAATACAACTTCAGCCCTTACCTCTCTTTGAAGAAATTCTACATCTCTGACACGGACAACAAATTCATAGGCGTTGGTTGCGCCACCTATGCCGAAATTCAGGGTTTCAATCGTGGTAGATATCAGTACACAGGTGGGTGCATTTCAAAATGTGAAAGCATGGACTTTGTTTCCAATTACTCATGCTCTGGTATAGGCTGTTGCCAAACACCTATTGCTAAAGGCATGACTTACTTTGACATAAGTGTAACTAGCTACAAGAATCAAACCGAAGTGTGGGACTTCAATTCCTGTAGCTATGCTTTCCTTGTCCAAGAGGGTAAGTTCAACTTCACCTCCGACATGTTGAGAGATTTATGGAATACCTCTCGGTTTCCGGTGGTTCTCGATTGGTCAATTGGAAATGAAAATTGCAGCATGATCCAGAAAAATGAATGTAAGGTGAACAGCAAGTGTGTGGATGTCCAGAATGGGCTCGGCTACCGCTGCAAGTGCAACGAAGGTTACGAAGGAAATCCATACCTAAATGGCTGCACTGACATTAATGAATGCGAGAATCCGGTGATTGCCAAGAACTGCAAACAAAGATGCATAAATAAAGAGGGGAACTATACTTGTGCTTGCCACAAAGGGTACCACAACAATGGCACAGAAGACTCGGTAGATTGTGTCGCTGACCAAACATTTGTTGTTCAAATTACACTTGGACTCGGTGTTGGCCTCATAGCCTTGTTAATAGGCAGCTCCTGGTTGTACTTAGTCTACAAAAGATGGAAGCTCATGAAGCTGAAGGAAAAGTTCTTCATCCAAAATGGAGGATTGATGTTGAAGCAACAGCTTTCGGAACGACAAGGTGGAGACCAAAGGGCCAAAATCTTCACCGAAGTCCAGCTTAAGAAGGCTACCAACCATTTCAGTGAAGCCAGAATCGTTGGCAAAGGAGGTTTTGGTACAGTTTACAAAGGGATCATAGTAGATGAAAAAGGAAAGGAAACAGTGGTTGCAATCAAGAAGTCCAAACTTGTGGATCGAAGCCAAATACAGCAATTCATAAATGAAGTACTCGTGCTCTCCCAAATCAACCATAGAAATGTGGTGAAGCTCTTGGGTTGTTGTTTCGAGACAGAAGTCCCACTACTAGTTTATGAGTTTGTCAACAATGGTACACTATTCGACTACATCCACAACAAAAGCAAGGCATGCAACTTTGCTTGGGAAAGTCGTTTAAGGATAGCTGCAGAAACTGCTGGAGTTCTATCACACTTGCACTCTGAGGCTTCTATTCCTATTATTCACAGAGATGTCAAGTCCACAAACATACTCCTGGATGATAATCTTACAGCAAAAGTGTCTGATTTCGGTGCCTCGAGATTGGTTCCCTCAGATCAAGCTCAGCTATCTACAATGGTGCAAGGAACAGTAGGGTATTTAGACCCTGAGTACTTGCAAACAAGCCAGCTGACTGACAAGAGTGATGTTTATAGCTTTGGGGTTGTTCTTGTGGAACTACTAACAGGAAAGAAGGCGCTTTCATTCGACAAGCCAGAGGAGGAGAGAAATCTAGCAATGTATTTTCTCTCTGCGCTGAAACATGATCGCTTGGTGCAAGTTATTGATGGTTGTATTCTGAATGAAGCAAATATTGAGCAGCTGAAGGAAGTTTCTGTCCTTGCAAAGAGGTGTTTGAGAGTAAAAGGAGAAGAAAGACCAACCATGAAGGAAGTGGCAATGGAGCTCGAGGGACTAAGAAGGATGGTGATGCATCACCCATGGGTTAACAATGATGAAACTGATGTACAGGAGATTGAGCACTTGCTTGGTGAAATAGCAATGGAAACTTTTAGTCATGGAGAGGGTGGCGTTATGAGTTCTGGATATGATACCATGAGGAACCAGATCATATTACCAGTTGATGATGGGACTCATGGGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase WAK

Protein Analysis

752

Amino Acids

84.28

Weight (kDa)

5.85

Isoelectric Point (pI)

35.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 32 - 87 1.1e-09 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 287 - 325 4.1e-06 Calcium-binding EGF domain
Pkinase PF00069 413 - 682 1.7e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 416 - 686 8.3e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 236
Acc65I GGTACC 1 cut(s) 954
AccB1I GGYRCC 2 cut(s) 954, 1682
AccB7I CCANNNNNTGG 1 cut(s) 79
AccI GTMKAC 1 cut(s) 1083
AciI CCGC 2 cut(s) 29, 805
AclWI GGATC 3 cut(s) 736, 1295, 1356
AcsI RAATTY 3 cut(s) 356, 383, 417
AcuI CTGAAG 3 cut(s) 316, 1130, 1994
AfaI GTAC 8 cut(s) 449, 956, 1076, 1273, 1383, 1486, 1763, 2118
AfiI CCNNNNNNNGG 3 cut(s) 79, 258, 597
AflII CTTAAG 1 cut(s) 1214
AflIII ACRYGT 1 cut(s) 663
AgsI TTSAA 7 cut(s) 352, 430, 468, 607, 649, 1016, 1150
AhlI ACTAGT 2 cut(s) 61, 1459
AjnI CCWGG 2 cut(s) 1067, 1644
Alw21I GWGCWC 3 cut(s) 1392, 2058, 2133
Alw26I GTCTC 3 cut(s) 3, 1173, 1436
AlwI GGATC 3 cut(s) 736, 1295, 1356
AlwNI CAGNNNCTG 2 cut(s) 1068, 1565
Ama87I CYCGRG 2 cut(s) 1687, 2057
AoxI GGCC 2 cut(s) 1039, 1188
ApeKI GCWGC 7 cut(s) 25, 735, 807, 852, 1062, 1556, 1969
ApoI RAATTY 3 cut(s) 356, 383, 417
ArsI GACNNNNNNTTYG 2 cut(s) 989, 1021
AseI ATTAAT 1 cut(s) 864
Asp700I GAANNNNTTC 1 cut(s) 356
Asp718I GGTACC 1 cut(s) 954
AspLEI GCGC 3 cut(s) 404, 1849, 1906
AspS9I GGNCC 1 cut(s) 1188
AsuHPI GGTGA 8 cut(s) 646, 775, 895, 1192, 1429, 2078, 2089, 2153
AvaI CYCGRG 2 cut(s) 1687, 2057
BanI GGYRCC 2 cut(s) 954, 1682
BanII GRGCYC 2 cut(s) 798, 2058
BarI GAAGNNNNNNTAC 4 cut(s) 344, 376, 1440, 1472
BauI CACGAG 1 cut(s) 1385
BbsI GAAGAC 1 cut(s) 981
Bbv12I GWGCWC 3 cut(s) 1392, 2058, 2133
BbvI GCAGC 7 cut(s) 12, 747, 794, 839, 1074, 1543, 1981
BccI CCATC 5 cut(s) 251, 1086, 1931, 2068, 2231
BceAI ACGGC 1 cut(s) 301
BciT130I CCWGG 2 cut(s) 1069, 1646
BcoDI GTCTC 3 cut(s) 3, 1173, 1436
BcuI ACTAGT 2 cut(s) 61, 1459
BfaI CTAG 5 cut(s) 62, 84, 573, 1460, 1883
BfmI CTRYAG 2 cut(s) 613, 1557
BfoI RGCGCY 1 cut(s) 1850
BfrI CTTAAG 1 cut(s) 1214
BisI GCNGC 8 cut(s) 26, 29, 736, 808, 853, 1063, 1557, 1970
BlpI GCTNAGC 1 cut(s) 1713
BlsI GCNGC 8 cut(s) 27, 30, 737, 809, 854, 1064, 1558, 1971
BmcAI AGTACT 2 cut(s) 1383, 1763
Bme1390I CCNGG 2 cut(s) 1069, 1646
BmeT110I CYCGRG 2 cut(s) 1687, 2057
BmgT120I GGNCC 1 cut(s) 1188
BmiI GGNNCC 4 cut(s) 956, 1684, 1698, 2213
BmrFI CCNGG 2 cut(s) 1069, 1646
BmsI GCATC 3 cut(s) 899, 2070, 2092
BoxI GACNNNNGTC 1 cut(s) 1448
BpiI GAAGAC 1 cut(s) 981
BpmI CTGGAG 1 cut(s) 1590
Bpu1102I GCTNAGC 1 cut(s) 1713
BsaBI GATNNNNATC 1 cut(s) 1653
BsaI GGTCTC 1 cut(s) 1173
BsaJI CCNNGG 2 cut(s) 288, 2090
BsaWI WCCGGW 2 cut(s) 697, 880
BsaXI ACNNNNNCTCC 2 cut(s) 641, 671
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 258, 597
Bse1I ACTGG 1 cut(s) 2228
Bse3DI GCAATG 3 cut(s) 1893, 2055, 2157
Bse8I GATNNNNATC 1 cut(s) 1653
BseBI CCWGG 2 cut(s) 1069, 1646
BseDI CCNNGG 2 cut(s) 288, 2090
BseGI GGATG 6 cut(s) 68, 787, 1125, 1500, 1654, 2079
BseJI GATNNNNATC 1 cut(s) 1653
BseLI CCNNNNNNNGG 3 cut(s) 79, 258, 597
BseMI GCAATG 3 cut(s) 1893, 2055, 2157
BseMII CTCAG 4 cut(s) 1584, 1716, 1727, 1749
BseNI ACTGG 1 cut(s) 2228
BseRI GAGGAG 2 cut(s) 1883, 1886
BseXI GCAGC 7 cut(s) 12, 747, 794, 839, 1074, 1543, 1981
BsgI GTGCAG 1 cut(s) 838
BshFI GGCC 2 cut(s) 1041, 1190
BshNI GGYRCC 2 cut(s) 954, 1682
BsiHKAI GWGCWC 3 cut(s) 1392, 2058, 2133
BsiHKCI CYCGRG 2 cut(s) 1687, 2057
BsiSI CCGG 2 cut(s) 698, 881
BslFI GGGAC 3 cut(s) 614, 1436, 2076
BslI CCNNNNNNNGG 3 cut(s) 79, 258, 597
BsmAI GTCTC 3 cut(s) 3, 1173, 1436
BsmFI GGGAC 3 cut(s) 614, 1436, 2076
BsmI GAATGC 2 cut(s) 120, 875
BsnI GGCC 2 cut(s) 1041, 1190
Bso31I GGTCTC 1 cut(s) 1173
BsoBI CYCGRG 2 cut(s) 1687, 2057
Bsp1286I GDGCHC 4 cut(s) 798, 1392, 2058, 2133
Bsp1407I TGTACA 1 cut(s) 2116
Bsp143I GATC 6 cut(s) 741, 1287, 1348, 1705, 1915, 2217
Bsp1720I GCTNAGC 1 cut(s) 1713
Bsp19I CCATGG 2 cut(s) 288, 2090
BspACI CCGC 2 cut(s) 29, 805
BspANI GGCC 2 cut(s) 1041, 1190
BspCNI CTCAG 4 cut(s) 1585, 1715, 1726, 1750
BspHI TCATGA 1 cut(s) 1101
BspLI GGNNCC 4 cut(s) 956, 1684, 1698, 2213
BspMAI CTGCAG 1 cut(s) 1561
BspPI GGATC 3 cut(s) 736, 1295, 1356
BspT107I GGYRCC 2 cut(s) 954, 1682
BspTI CTTAAG 1 cut(s) 1214
BspTNI GGTCTC 1 cut(s) 1173
BsrDI GCAATG 3 cut(s) 1893, 2055, 2157
BsrGI TGTACA 1 cut(s) 2116
BsrI ACTGG 1 cut(s) 2228
BssECI CCNNGG 2 cut(s) 288, 2090
BssMI GATC 6 cut(s) 741, 1287, 1348, 1705, 1915, 2217
BssSI CACGAG 1 cut(s) 1385
BssT1I CCWWGG 2 cut(s) 288, 2090
Bst2BI CACGAG 1 cut(s) 1385
Bst2UI CCWGG 2 cut(s) 1069, 1646
Bst4CI ACNGT 4 cut(s) 79, 1276, 1318, 1741
BstAFI CTTAAG 1 cut(s) 1214
BstAPI GCANNNNNTGC 3 cut(s) 37, 1521, 1565
BstAUI TGTACA 1 cut(s) 2116
BstC8I GCNNGC 3 cut(s) 943, 1522, 1778
BstDEI CTNAG 6 cut(s) 1078, 1593, 1702, 1713, 1758, 2066
BstDSI CCRYGG 2 cut(s) 288, 2090
BstF5I GGATG 6 cut(s) 68, 787, 1125, 1500, 1654, 2079
BstH2I RGCGCY 1 cut(s) 1850
BstHHI GCGC 3 cut(s) 404, 1849, 1906
BstKTI GATC 6 cut(s) 744, 1290, 1351, 1708, 1918, 2220
BstMAI GTCTC 3 cut(s) 3, 1173, 1436
BstMBI GATC 6 cut(s) 741, 1287, 1348, 1705, 1915, 2217
BstMWI GCNNNNNNNGC 9 cut(s) 37, 117, 399, 410, 807, 813, 1105, 1521, 1565
BstNI CCWGG 2 cut(s) 1069, 1646
BstNSI RCATGY 2 cut(s) 667, 1524
BstPAI GACNNNNGTC 1 cut(s) 1448
BstSCI CCNGG 2 cut(s) 1067, 1644
BstSFI CTRYAG 2 cut(s) 613, 1557
BstV1I GCAGC 7 cut(s) 12, 747, 794, 839, 1074, 1543, 1981
BstV2I GAAGAC 1 cut(s) 981
BstXI CCANNNNNNTGG 2 cut(s) 965, 1345
BsuRI GGCC 2 cut(s) 1041, 1190
BtgI CCRYGG 2 cut(s) 288, 2090
BtsCI GGATG 6 cut(s) 68, 787, 1125, 1500, 1654, 2079
BtsI GCAGTG 1 cut(s) 118
BtsIMutI CAGTG 5 cut(s) 84, 118, 855, 1243, 1323
Cac8I GCNNGC 3 cut(s) 943, 1522, 1778
CaiI CAGNNNCTG 2 cut(s) 1068, 1565
CciI TCATGA 1 cut(s) 1101
CfoI GCGC 3 cut(s) 404, 1849, 1906
Cfr13I GGNCC 1 cut(s) 1188
Csp6I GTAC 8 cut(s) 448, 955, 1075, 1272, 1382, 1485, 1762, 2117
CviQI GTAC 8 cut(s) 448, 955, 1075, 1272, 1382, 1485, 1762, 2117
DdeI CTNAG 6 cut(s) 1078, 1593, 1702, 1713, 1758, 2066
DpnI GATC 6 cut(s) 743, 1289, 1350, 1707, 1917, 2219
DpnII GATC 6 cut(s) 741, 1287, 1348, 1705, 1915, 2217
DrdI GACNNNNNNGTC 1 cut(s) 236
DseDI GACNNNNNNGTC 1 cut(s) 236
Ecl136II GAGCTC 1 cut(s) 2056
Eco130I CCWWGG 2 cut(s) 288, 2090
Eco24I GRGCYC 2 cut(s) 798, 2058
Eco31I GGTCTC 1 cut(s) 1173
Eco32I GATATC 1 cut(s) 443
Eco53kI GAGCTC 1 cut(s) 2056
Eco57I CTGAAG 3 cut(s) 316, 1130, 1994
Eco88I CYCGRG 2 cut(s) 1687, 2057
EcoICRI GAGCTC 1 cut(s) 2056
EcoRII CCWGG 2 cut(s) 1067, 1644
EcoRV GATATC 1 cut(s) 443
EcoT14I CCWWGG 2 cut(s) 288, 2090
EcoT22I ATGCAT 2 cut(s) 914, 2085
EcoT38I GRGCYC 2 cut(s) 798, 2058
ErhI CCWWGG 2 cut(s) 288, 2090
FalI AAGNNNNNCTT 4 cut(s) 635, 667, 1197, 1229
FaqI GGGAC 3 cut(s) 614, 1436, 2076
FblI GTMKAC 1 cut(s) 1083
Fnu4HI GCNGC 8 cut(s) 26, 29, 736, 808, 853, 1063, 1557, 1970
FokI GGATG 6 cut(s) 55, 794, 1112, 1487, 1661, 2086
FriOI GRGCYC 2 cut(s) 798, 2058
Fsp4HI GCNGC 8 cut(s) 26, 29, 736, 808, 853, 1063, 1557, 1970
FspBI CTAG 5 cut(s) 62, 84, 573, 1460, 1883
GlaI GCGC 3 cut(s) 403, 1848, 1905
GluI GCNGC 8 cut(s) 26, 29, 736, 808, 853, 1063, 1557, 1970
GsuI CTGGAG 1 cut(s) 1590
HaeII RGCGCY 1 cut(s) 1850
HaeIII GGCC 2 cut(s) 1041, 1190
HapII CCGG 2 cut(s) 698, 881
HhaI GCGC 3 cut(s) 404, 1849, 1906
Hin6I GCGC 3 cut(s) 402, 1847, 1904
HinP1I GCGC 3 cut(s) 402, 1847, 1904
HincII GTYRAC 2 cut(s) 1477, 2098
HindII GTYRAC 2 cut(s) 1477, 2098
HinfI GANTC 7 cut(s) 305, 583, 877, 977, 1029, 1248, 2242
HpaI GTTAAC 1 cut(s) 2098
HpaII CCGG 2 cut(s) 698, 881
HphI GGTGA 8 cut(s) 646, 775, 895, 1192, 1429, 2078, 2089, 2153
Hpy166II GTNNAC 8 cut(s) 450, 766, 1084, 1279, 1477, 1487, 1632, 2098
Hpy188I TCNGA 9 cut(s) 137, 322, 370, 661, 1165, 1594, 1675, 1705, 1950
Hpy188III TCNNGA 8 cut(s) 707, 745, 787, 1102, 1330, 1441, 1689, 2193
Hpy8I GTNNAC 8 cut(s) 450, 766, 1084, 1279, 1477, 1487, 1632, 2098
Hpy99I CGWCG 1 cut(s) 322
HpyAV CCTTC 8 cut(s) 815, 824, 1105, 1213, 1837, 1969, 2032, 2064
HpyCH4III ACNGT 4 cut(s) 79, 1276, 1318, 1741
HpyF10VI GCNNNNNNNGC 9 cut(s) 37, 117, 399, 410, 807, 813, 1105, 1521, 1565
HpyF3I CTNAG 6 cut(s) 1078, 1593, 1702, 1713, 1758, 2066
HspAI GCGC 3 cut(s) 402, 1847, 1904
KpnI GGTACC 1 cut(s) 958
KspAI GTTAAC 1 cut(s) 2098
Kzo9I GATC 6 cut(s) 741, 1287, 1348, 1705, 1915, 2217
LmnI GCTCC 2 cut(s) 1070, 2053
Lsp1109I GCAGC 7 cut(s) 12, 747, 794, 839, 1074, 1543, 1981
LweI GCATC 3 cut(s) 899, 2070, 2092
MaeI CTAG 5 cut(s) 62, 84, 573, 1460, 1883
MaeIII GTNAC 5 cut(s) 152, 227, 238, 568, 824
MalI GATC 6 cut(s) 743, 1289, 1350, 1707, 1917, 2219
MboI GATC 6 cut(s) 741, 1287, 1348, 1705, 1915, 2217
MboII GAAGA 6 cut(s) 209, 364, 986, 1114, 1189, 2033
MfeI CAATTG 2 cut(s) 32, 717
MhlI GDGCHC 4 cut(s) 798, 1392, 2058, 2133
MlyI GAGTC 3 cut(s) 971, 1023, 2236
MmeI TCCRAC 2 cut(s) 300, 684
Mph1103I ATGCAT 2 cut(s) 914, 2085
MroXI GAANNNNTTC 1 cut(s) 356
MseI TTAA 5 cut(s) 864, 1055, 1215, 1547, 2097
MslI CAYNNNNRTG 4 cut(s) 963, 1236, 1410, 1620
MspA1I CMGCKG 3 cut(s) 807, 1780, 1972
MspCI CTTAAG 1 cut(s) 1214
MspI CCGG 2 cut(s) 698, 881
MspR9I CCNGG 2 cut(s) 1069, 1646
MunI CAATTG 2 cut(s) 32, 717
Mva1269I GAATGC 2 cut(s) 120, 875
MvaI CCWGG 2 cut(s) 1069, 1646
MwoI GCNNNNNNNGC 9 cut(s) 37, 117, 399, 410, 807, 813, 1105, 1521, 1565
NcoI CCATGG 2 cut(s) 288, 2090
NdeII GATC 6 cut(s) 741, 1287, 1348, 1705, 1915, 2217
NlaIV GGNNCC 4 cut(s) 956, 1684, 1698, 2213
NmeAIII GCCGAG 1 cut(s) 777
NmuCI GTSAC 2 cut(s) 227, 238
NsiI ATGCAT 2 cut(s) 914, 2085
NspI RCATGY 2 cut(s) 667, 1524
PaeI GCATGC 1 cut(s) 1524
PaeR7I CTCGAG 2 cut(s) 1687, 2057
PagI TCATGA 1 cut(s) 1101
PciI ACATGT 1 cut(s) 663
PcsI WCGNNNNNNNCGW 1 cut(s) 825
PctI GAATGC 2 cut(s) 120, 875
PdmI GAANNNNTTC 1 cut(s) 356
PfeI GAWTC 4 cut(s) 305, 583, 877, 1248
PflMI CCANNNNNTGG 1 cut(s) 79
PfoI TCCNGGA 1 cut(s) 1644
PkrI GCNGC 8 cut(s) 27, 30, 737, 809, 854, 1064, 1558, 1971
PleI GAGTC 3 cut(s) 971, 1023, 2236
PpsI GAGTC 3 cut(s) 971, 1023, 2236
PscI ACATGT 1 cut(s) 663
PshAI GACNNNNGTC 1 cut(s) 1448
PshBI ATTAAT 1 cut(s) 864
Psp124BI GAGCTC 1 cut(s) 2058
Psp6I CCWGG 2 cut(s) 1067, 1644
PspGI CCWGG 2 cut(s) 1067, 1644
PspN4I GGNNCC 4 cut(s) 956, 1684, 1698, 2213
PspPI GGNCC 1 cut(s) 1188
PspXI VCTCGAGB 1 cut(s) 2057
PstI CTGCAG 1 cut(s) 1561
PstNI CAGNNNCTG 2 cut(s) 1068, 1565
PvuII CAGCTG 2 cut(s) 1780, 1972
RsaI GTAC 8 cut(s) 449, 956, 1076, 1273, 1383, 1486, 1763, 2118
RsaNI GTAC 8 cut(s) 448, 955, 1075, 1272, 1382, 1485, 1762, 2117
RseI CAYNNNNRTG 4 cut(s) 963, 1236, 1410, 1620
SacI GAGCTC 1 cut(s) 2058
SaqAI TTAA 5 cut(s) 864, 1055, 1215, 1547, 2097
SatI GCNGC 8 cut(s) 26, 29, 736, 808, 853, 1063, 1557, 1970
Sau3AI GATC 6 cut(s) 741, 1287, 1348, 1705, 1915, 2217
Sau96I GGNCC 1 cut(s) 1188
ScaI AGTACT 2 cut(s) 1383, 1763
SchI GAGTC 3 cut(s) 971, 1023, 2236
ScrFI CCNGG 2 cut(s) 1069, 1646
SduI GDGCHC 4 cut(s) 798, 1392, 2058, 2133
SfaNI GCATC 3 cut(s) 899, 2070, 2092
SfcI CTRYAG 2 cut(s) 613, 1557
Sfr274I CTCGAG 2 cut(s) 1687, 2057
SlaI CTCGAG 2 cut(s) 1687, 2057
SmiMI CAYNNNNRTG 4 cut(s) 963, 1236, 1410, 1620
SmlI CTYRAG 3 cut(s) 1214, 1687, 2057
SmoI CTYRAG 3 cut(s) 1214, 1687, 2057
SpeI ACTAGT 2 cut(s) 61, 1459
SphI GCATGC 1 cut(s) 1524
SsiI CCGC 2 cut(s) 29, 805
SspI AATATT 1 cut(s) 1963
SspMI CTAG 5 cut(s) 62, 84, 573, 1460, 1883
SstI GAGCTC 1 cut(s) 2058
StyD4I CCNGG 2 cut(s) 1067, 1644
StyI CCWWGG 2 cut(s) 288, 2090
TaaI ACNGT 4 cut(s) 79, 1276, 1318, 1741
TaqI TCGA 8 cut(s) 220, 708, 1351, 1440, 1494, 1688, 1857, 2058
TatI WGTACW 5 cut(s) 447, 1074, 1381, 1761, 2116
TauI GCSGC 1 cut(s) 31
TfiI GAWTC 4 cut(s) 305, 583, 877, 1248
Tru1I TTAA 5 cut(s) 864, 1055, 1215, 1547, 2097
Tru9I TTAA 5 cut(s) 864, 1055, 1215, 1547, 2097
TscAI CASTG 5 cut(s) 84, 118, 862, 1243, 1323
TseFI GTSAC 2 cut(s) 227, 238
TseI GCWGC 7 cut(s) 25, 735, 807, 852, 1062, 1556, 1969
Tsp45I GTSAC 2 cut(s) 227, 238
TspGWI ACGGA 2 cut(s) 120, 389
TspRI CASTG 5 cut(s) 84, 118, 862, 1243, 1323
Van91I CCANNNNNTGG 1 cut(s) 79
Vha464I CTTAAG 1 cut(s) 1214
VspI ATTAAT 1 cut(s) 864
XapI RAATTY 3 cut(s) 356, 383, 417
XceI RCATGY 2 cut(s) 667, 1524
XcmI CCANNNNNNNNNTGG 2 cut(s) 104, 2041
XhoI CTCGAG 2 cut(s) 1687, 2057
XmiI GTMKAC 1 cut(s) 1083
XmnI GAANNNNTTC 1 cut(s) 356
XspI CTAG 5 cut(s) 62, 84, 573, 1460, 1883
ZrmI AGTACT 2 cut(s) 1383, 1763
Zsp2I ATGCAT 2 cut(s) 914, 2085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.