Rroxscaffold_1G00060900

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000066
Physical Location & Seq
Reverse (-)
83121392 .. 83124629
3238 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00060900.1

Sequence Viewer

Length: 2232 bp
ATGGCGTTGCGACTCTCTGTGGTGGCAGTCCTACTCCTGTTATCGGCGACTAGTACTACTACGACAACAGCAGCTCCTGCTCAAGCCCTGCCTGGCTGCTCTGACAAGTGCGGCGATCTCAGAATTCCATATCCATTTGGCATGGAGGAGAATTGTTCCCTGGCAGATAGCTTCTTCATTGAGTGTAGCCAGGGAATAGCATACTTGGGCAACGAATCCACCAATATCATTGTTACTAAAATATCCGTAGACGTTGGGGAGTTGCAAATACGGAACTATGTAGGGCGAGATTGTTTTGATCAAGAGGGCAATCGAACTTACAGAAACAGACCGTTGTTCCGGATGAGACCTCCATACACCATATCCGATACCAAAAACAAGTTCTACGCCGTTGGTTGTGACACGTACGCAGTCTTGCACGGCTTCCGAGGGGAAGATGAATTCCTTACTGGGTGCGTGTCAGTATGTTACAGCCTTGGCAGTGTTGATAAGAACTCTTGCTCTGGCGCTGGGTGTTGCCAGACTAACATCCCCAGTGGACTGCACAATCGTACTGTGAGTTTAAGGACCTACTTCAATCATTCGTATGTGTGGAACTTTAACCCCTGCAGCTATGCCTTCATTGCGGAACAAGGCCAGTTCCATTTCTCCCCTGCAAGTTTTGAACAACTGAATGGCACTGAACGGCTTCCAATGGTACTTAATTGGGCCATTGGGAATCACAGTGACCCTTGTGATGAAGCTCGAAAGAGGAAGGATTTCGCTTGCAAGGCTCATAGCAAGTGCATCAACAGGCCTATCAATGGGTCTGTAGGTTACTTGTGCCAGTGCTTGCCTGGTTACGAAGGAAACCCCTACCATCCAGATGGTTGCCAAGATATTGATGAGTGCAAGGCTCCAAACCCCTGCCAGCATGGAAAGTGCTCAAATTCACCAGGAAATTACTCTTGTTCATGTCACAAAGGTTACAAAAACGACCGCATGAATGACAAAATTTGCATGAAGGACGATCCCCCGCTTACTGTTCTATTGGGTGTTGGTGTAGGCTTCTTTGTTTTATTCGTTGGAATTTCATGGTTATGGTGGGGAAATAAGAAAAGACAGTTCATCAAATTGAAAGCAAAGTACTTCAGAGAAAATGGAGGCGTATTGTTACAACAACAACTTGCCAGTCATGGAGGTTCCGTGGAAACAACAAAAATTTTTAGCGCAGAAGAACTTCAGAAGGCTACAAACAATTACCATGAAAGTAGAGTTCTTGGCAAAGGAAGCTATGGAATAGTATACAAAGGTATACTAGCAGATAACGAAGTGATTGCCATAAAGAAGTCAAGAGTTTGTGCCGGCAGTCAGAATGGGCAATTTGTCAACGAGATAATCATTCTTTCTCAAATCAACCACAAAAATGTTGTGAAGCTTTTAGGTTGTTGTTTAGAAACAGAAGTGCCCATACTTGTATACGAGTTCATCAGCCATGGCACTCTTCATGAGCACATTCATAAGAAAAGATCATCACTCTCATTTGAATTACGAATGAAGATAGCAGCTGAAGCAGCAGGAGCACTAGCCCATTTGCACTCCTCAATTTCCACACCAATCATACATCGAGATGTGAAAGCGGCAAATATTCTATTAGATGATAATTATATGGCGAAAGTGTCAGACTTTGGAGCTTCACGATTGATTTCTTCAGGTGAAGCTGGAATCCAAACTGTAGTGCTCGGGACAATCGGATACCTGGACCCTGAATATCTGCAATCAAACCAACTAACAGAAAAGAGTGACGTTTACAGCTTTGGGGTTGTCTTGGTTGAGCTTTTAACAAGCAAAGTGGCAGTTTCTAGAGATAATAGATGCTTAGCAAGCATCTTTATTGCTTCCATGGAAGAAGATTGCTTAAATCAAGTGCTTGATTATGAGATAGTGAATGAAGGAAATATTGAGATGGTGAAAAATGTGGCCACTCTTGCAAGAAGATGTTTGAGGGTAAAAGGCGAGGAGAGGCCTACCATGAGAGAAGTAGCCATGGAGCTAGAAGGAATGATTATGACAAACCATTTATGGGGTTCTGCAGATCATTACTTCCCAGAAGAGACTGAGCACCTGCTTGGATCACCTAATAATTCCAGGGATTATGCTGCGAATGTTGATTGTGGTGGTGGTCCTGGTACCGCCAGTGGGTCTAGCATGCAAATAGACATGTCAATGTCTTCATATGCTGAGGGACGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

743

Amino Acids

81.92

Weight (kDa)

6.28

Isoelectric Point (pI)

38.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 33 - 90 1.4e-07 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 293 - 328 1.3e-06 Calcium-binding EGF domain
Pkinase PF00069 414 - 674 2.5e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 417 - 678 1.5e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2112
Acc36I ACCTGC 1 cut(s) 2112
Acc65I GGTACC 1 cut(s) 2168
AccB1I GGYRCC 1 cut(s) 2168
AccI GTMKAC 4 cut(s) 249, 1284, 1294, 1458
AccIII TCCGGA 1 cut(s) 339
AciI CCGC 6 cut(s) 111, 626, 979, 1016, 1619, 2172
AclWI GGATC 2 cut(s) 1004, 2119
AcoI YGGCCR 1 cut(s) 1959
AcsI RAATTY 6 cut(s) 123, 440, 928, 993, 1068, 1200
AcuI CTGAAG 4 cut(s) 1114, 1205, 1569, 1674
AfaI GTAC 6 cut(s) 55, 407, 553, 699, 1127, 2170
AfiI CCNNNNNNNGG 4 cut(s) 43, 803, 2062, 2178
AflIII ACRYGT 2 cut(s) 402, 2199
AgsI TTSAA 4 cut(s) 577, 665, 1117, 1526
AhlI ACTAGT 1 cut(s) 50
AjnI CCWGG 8 cut(s) 91, 159, 189, 835, 934, 1737, 2126, 2164
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
Alw21I GWGCWC 5 cut(s) 926, 1494, 1564, 1722, 2103
Alw26I GTCTC 2 cut(s) 340, 2087
AlwI GGATC 2 cut(s) 1004, 2119
AlwNI CAGNNNCTG 1 cut(s) 77
Ama87I CYCGRG 1 cut(s) 1721
Aor13HI TCCGGA 1 cut(s) 339
AoxI GGCC 5 cut(s) 634, 708, 794, 1959, 2003
ApeKI GCWGC 6 cut(s) 71, 96, 609, 1544, 1553, 2138
ApoI RAATTY 6 cut(s) 123, 440, 928, 993, 1068, 1200
Asp700I GAANNNNTTC 3 cut(s) 687, 758, 1218
Asp718I GGTACC 1 cut(s) 2168
AspLEI GCGC 2 cut(s) 509, 1211
AspS9I GGNCC 4 cut(s) 567, 708, 1741, 2162
AsuHPI GGTGA 4 cut(s) 924, 1706, 1960, 2106
AvaI CYCGRG 1 cut(s) 1721
AvaII GGWCC 3 cut(s) 567, 1741, 2162
BaeGI GKGCMC 1 cut(s) 1449
BalI TGGCCA 1 cut(s) 1961
BanI GGYRCC 1 cut(s) 2168
BarI GAAGNNNNNNTAC 2 cut(s) 1434, 1466
BbsI GAAGAC 1 cut(s) 2202
Bbv12I GWGCWC 5 cut(s) 926, 1494, 1564, 1722, 2103
BbvCI CCTCAGC 1 cut(s) 2220
BbvI GCAGC 6 cut(s) 83, 83, 621, 1556, 1565, 2125
BccI CCATC 3 cut(s) 860, 867, 1939
BceAI ACGGC 3 cut(s) 374, 436, 701
BcgI CGANNNNNNTGC 2 cut(s) 1298, 1332
BciT130I CCWGG 8 cut(s) 93, 161, 191, 837, 936, 1739, 2128, 2166
BciVI GTATCC 1 cut(s) 1727
BclI TGATCA 1 cut(s) 298
BcoDI GTCTC 2 cut(s) 340, 2087
BcuI ACTAGT 1 cut(s) 50
BfaI CTAG 6 cut(s) 51, 1298, 1565, 1842, 2033, 2184
BfmI CTRYAG 4 cut(s) 607, 810, 1713, 2070
BfoI RGCGCY 1 cut(s) 510
BfuAI ACCTGC 1 cut(s) 2112
BfuI GTATCC 1 cut(s) 1727
BisI GCNGC 8 cut(s) 72, 97, 112, 610, 1545, 1554, 1620, 2139
BlpI GCTNAGC 1 cut(s) 1858
BlsI GCNGC 8 cut(s) 73, 98, 113, 611, 1546, 1555, 1621, 2140
BmcAI AGTACT 2 cut(s) 55, 1127
Bme1390I CCNGG 8 cut(s) 93, 161, 191, 837, 936, 1739, 2128, 2166
Bme18I GGWCC 3 cut(s) 567, 1741, 2162
BmeT110I CYCGRG 1 cut(s) 1721
BmgT120I GGNCC 4 cut(s) 567, 708, 1741, 2162
BmiI GGNNCC 4 cut(s) 897, 1183, 1743, 2170
BmrFI CCNGG 8 cut(s) 93, 161, 191, 837, 936, 1739, 2128, 2166
BmrI ACTGGG 2 cut(s) 459, 528
BmsI GCATC 3 cut(s) 795, 1844, 1875
BmuI ACTGGG 2 cut(s) 459, 528
BpiI GAAGAC 1 cut(s) 2202
Bpu10I CCTNAGC 1 cut(s) 2220
Bpu1102I GCTNAGC 1 cut(s) 1858
BpuEI CTTGAG 1 cut(s) 66
BsaAI YACGTR 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 340
BsaJI CCNNGG 9 cut(s) 159, 190, 427, 475, 1185, 1474, 1881, 2025, 2127
BsaWI WCCGGW 1 cut(s) 339
BsaXI ACNNNNNCTCC 4 cut(s) 58, 88, 1170, 1200
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 803, 2062, 2178
Bse118I RCCGGY 1 cut(s) 1343
Bse1I ACTGG 6 cut(s) 454, 534, 637, 826, 1170, 2175
Bse3DI GCAATG 1 cut(s) 621
BseAI TCCGGA 1 cut(s) 339
BseBI CCWGG 8 cut(s) 93, 161, 191, 837, 936, 1739, 2128, 2166
BseDI CCNNGG 9 cut(s) 159, 190, 427, 475, 1185, 1474, 1881, 2025, 2127
BseGI GGATG 3 cut(s) 348, 528, 859
BseLI CCNNNNNNNGG 4 cut(s) 43, 803, 2062, 2178
BseMI GCAATG 1 cut(s) 621
BseMII CTCAG 3 cut(s) 133, 2088, 2211
BseNI ACTGG 6 cut(s) 454, 534, 637, 826, 1170, 2175
BseRI GAGGAG 3 cut(s) 161, 1570, 2012
BseSI GKGCMC 1 cut(s) 1449
BseXI GCAGC 6 cut(s) 83, 83, 621, 1556, 1565, 2125
BseYI CCCAGC 1 cut(s) 509
BsgI GTGCAG 1 cut(s) 527
Bsh1285I CGRYCG 1 cut(s) 979
BshFI GGCC 5 cut(s) 636, 710, 796, 1961, 2005
BshNI GGYRCC 1 cut(s) 2168
BsiEI CGRYCG 1 cut(s) 979
BsiHKAI GWGCWC 5 cut(s) 926, 1494, 1564, 1722, 2103
BsiHKCI CYCGRG 1 cut(s) 1721
BsiSI CCGG 2 cut(s) 340, 1344
BsiWI CGTACG 1 cut(s) 405
BslFI GGGAC 1 cut(s) 1738
BslI CCNNNNNNNGG 4 cut(s) 43, 803, 2062, 2178
BsmAI GTCTC 2 cut(s) 340, 2087
BsmFI GGGAC 1 cut(s) 1738
BsnI GGCC 5 cut(s) 636, 710, 796, 1961, 2005
Bso31I GGTCTC 1 cut(s) 340
BsoBI CYCGRG 1 cut(s) 1721
Bsp1286I GDGCHC 6 cut(s) 926, 1449, 1494, 1564, 1722, 2103
Bsp13I TCCGGA 1 cut(s) 339
Bsp143I GATC 6 cut(s) 115, 298, 1009, 1508, 2074, 2111
Bsp1720I GCTNAGC 1 cut(s) 1858
Bsp19I CCATGG 3 cut(s) 1474, 1881, 2025
BspACI CCGC 6 cut(s) 111, 626, 979, 1016, 1619, 2172
BspANI GGCC 5 cut(s) 636, 710, 796, 1961, 2005
BspCNI CTCAG 3 cut(s) 132, 2089, 2212
BspEI TCCGGA 1 cut(s) 339
BspHI TCATGA 1 cut(s) 1486
BspLI GGNNCC 4 cut(s) 897, 1183, 1743, 2170
BspMAI CTGCAG 2 cut(s) 611, 2074
BspMI ACCTGC 1 cut(s) 2112
BspPI GGATC 2 cut(s) 1004, 2119
BspT107I GGYRCC 1 cut(s) 2168
BspTNI GGTCTC 1 cut(s) 340
BsrDI GCAATG 1 cut(s) 621
BsrFI RCCGGY 1 cut(s) 1343
BsrI ACTGG 6 cut(s) 454, 534, 637, 826, 1170, 2175
BssAI RCCGGY 1 cut(s) 1343
BssECI CCNNGG 9 cut(s) 159, 190, 427, 475, 1185, 1474, 1881, 2025, 2127
BssMI GATC 6 cut(s) 115, 298, 1009, 1508, 2074, 2111
BssNAI GTATAC 3 cut(s) 1285, 1295, 1459
BssT1I CCWWGG 4 cut(s) 475, 1474, 1881, 2025
Bst1107I GTATAC 3 cut(s) 1285, 1295, 1459
Bst2UI CCWGG 8 cut(s) 93, 161, 191, 837, 936, 1739, 2128, 2166
Bst4CI ACNGT 6 cut(s) 333, 556, 725, 1024, 1104, 1714
Bst6I CTCTTC 2 cut(s) 1488, 2085
BstAPI GCANNNNNTGC 1 cut(s) 77
BstBAI YACGTR 1 cut(s) 405
BstC8I GCNNGC 6 cut(s) 766, 833, 911, 1345, 1864, 2189
BstDEI CTNAG 4 cut(s) 119, 1858, 2097, 2220
BstDSI CCRYGG 4 cut(s) 1185, 1474, 1881, 2025
BstF5I GGATG 3 cut(s) 348, 528, 859
BstH2I RGCGCY 1 cut(s) 510
BstHHI GCGC 2 cut(s) 509, 1211
BstKTI GATC 6 cut(s) 118, 301, 1012, 1511, 2077, 2114
BstMAI GTCTC 2 cut(s) 340, 2087
BstMBI GATC 6 cut(s) 115, 298, 1009, 1508, 2074, 2111
BstMCI CGRYCG 1 cut(s) 979
BstMWI GCNNNNNNNGC 9 cut(s) 77, 623, 770, 1269, 1550, 1553, 1559, 1863, 1967
BstNI CCWGG 8 cut(s) 93, 161, 191, 837, 936, 1739, 2128, 2166
BstNSI RCATGY 2 cut(s) 2191, 2203
BstSCI CCNGG 8 cut(s) 91, 159, 189, 835, 934, 1737, 2126, 2164
BstSFI CTRYAG 4 cut(s) 607, 810, 1713, 2070
BstSLI GKGCMC 1 cut(s) 1449
BstV1I GCAGC 6 cut(s) 83, 83, 621, 1556, 1565, 2125
BstV2I GAAGAC 1 cut(s) 2202
BstXI CCANNNNNNTGG 1 cut(s) 866
BstZ17I GTATAC 3 cut(s) 1285, 1295, 1459
BsuI GTATCC 1 cut(s) 1727
BsuRI GGCC 5 cut(s) 636, 710, 796, 1961, 2005
BtgI CCRYGG 4 cut(s) 1185, 1474, 1881, 2025
BtsCI GGATG 3 cut(s) 348, 528, 859
BtsI GCAGTG 1 cut(s) 487
BtsIMutI CAGTG 6 cut(s) 487, 541, 678, 730, 833, 2182
BveI ACCTGC 1 cut(s) 2112
Cac8I GCNNGC 6 cut(s) 766, 833, 911, 1345, 1864, 2189
CaiI CAGNNNCTG 1 cut(s) 77
CciI TCATGA 1 cut(s) 1486
CfoI GCGC 2 cut(s) 509, 1211
Cfr10I RCCGGY 1 cut(s) 1343
Cfr13I GGNCC 4 cut(s) 567, 708, 1741, 2162
Csp6I GTAC 6 cut(s) 54, 406, 552, 698, 1126, 2169
CspCI CAANNNNNGTGG 2 cut(s) 1812, 1847
CviQI GTAC 6 cut(s) 54, 406, 552, 698, 1126, 2169
DdeI CTNAG 4 cut(s) 119, 1858, 2097, 2220
DpnI GATC 6 cut(s) 117, 300, 1011, 1510, 2076, 2113
DpnII GATC 6 cut(s) 115, 298, 1009, 1508, 2074, 2111
EaeI YGGCCR 1 cut(s) 1959
Eam1104I CTCTTC 2 cut(s) 1488, 2085
EarI CTCTTC 2 cut(s) 1488, 2085
Eco130I CCWWGG 4 cut(s) 475, 1474, 1881, 2025
Eco147I AGGCCT 2 cut(s) 796, 2005
Eco31I GGTCTC 1 cut(s) 340
Eco47I GGWCC 3 cut(s) 567, 1741, 2162
Eco57I CTGAAG 4 cut(s) 1114, 1205, 1569, 1674
Eco88I CYCGRG 1 cut(s) 1721
EcoO109I RGGNCCY 1 cut(s) 567
EcoRI GAATTC 2 cut(s) 123, 440
EcoRII CCWGG 8 cut(s) 91, 159, 189, 835, 934, 1737, 2126, 2164
EcoT14I CCWWGG 4 cut(s) 475, 1474, 1881, 2025
ErhI CCWWGG 4 cut(s) 475, 1474, 1881, 2025
FaqI GGGAC 1 cut(s) 1738
FauI CCCGC 1 cut(s) 1023
FauNDI CATATG 1 cut(s) 2215
FbaI TGATCA 1 cut(s) 298
FblI GTMKAC 4 cut(s) 249, 1284, 1294, 1458
Fnu4HI GCNGC 8 cut(s) 72, 97, 112, 610, 1545, 1554, 1620, 2139
FokI GGATG 3 cut(s) 355, 515, 846
Fsp4HI GCNGC 8 cut(s) 72, 97, 112, 610, 1545, 1554, 1620, 2139
FspBI CTAG 6 cut(s) 51, 1298, 1565, 1842, 2033, 2184
GlaI GCGC 2 cut(s) 508, 1210
GluI GCNGC 8 cut(s) 72, 97, 112, 610, 1545, 1554, 1620, 2139
GsaI CCCAGC 1 cut(s) 513
HaeII RGCGCY 1 cut(s) 510
HaeIII GGCC 5 cut(s) 636, 710, 796, 1961, 2005
HapII CCGG 2 cut(s) 340, 1344
HhaI GCGC 2 cut(s) 509, 1211
Hin6I GCGC 2 cut(s) 507, 1209
HinP1I GCGC 2 cut(s) 507, 1209
HincII GTYRAC 1 cut(s) 1369
HindII GTYRAC 1 cut(s) 1369
HindIII AAGCTT 1 cut(s) 1415
HinfI GANTC 4 cut(s) 12, 215, 718, 1704
HpaII CCGG 2 cut(s) 340, 1344
HphI GGTGA 4 cut(s) 924, 1706, 1960, 2106
Hpy166II GTNNAC 7 cut(s) 250, 539, 1285, 1295, 1369, 1459, 1789
Hpy188I TCNGA 9 cut(s) 103, 122, 367, 428, 1133, 1224, 1353, 1663, 1733
Hpy188III TCNNGA 9 cut(s) 302, 340, 863, 1332, 1487, 1607, 1677, 1723, 1842
Hpy8I GTNNAC 7 cut(s) 250, 539, 1285, 1295, 1369, 1459, 1789
HpyAV CCTTC 7 cut(s) 628, 748, 839, 997, 1219, 1925, 2030
HpyCH4III ACNGT 6 cut(s) 333, 556, 725, 1024, 1104, 1714
HpyCH4IV ACGT 3 cut(s) 252, 404, 1785
HpyF10VI GCNNNNNNNGC 9 cut(s) 77, 623, 770, 1269, 1550, 1553, 1559, 1863, 1967
HpyF3I CTNAG 4 cut(s) 119, 1858, 2097, 2220
HpySE526I ACGT 3 cut(s) 252, 404, 1785
HspAI GCGC 2 cut(s) 507, 1209
Kpn2I TCCGGA 1 cut(s) 339
KpnI GGTACC 1 cut(s) 2172
KroI GCCGGC 1 cut(s) 1343
KroNI GCCGGC 1 cut(s) 1345
Ksp22I TGATCA 1 cut(s) 298
Kzo9I GATC 6 cut(s) 115, 298, 1009, 1508, 2074, 2111
LmnI GCTCC 5 cut(s) 79, 901, 1559, 1670, 2029
Lsp1109I GCAGC 6 cut(s) 83, 83, 621, 1556, 1565, 2125
LweI GCATC 3 cut(s) 795, 1844, 1875
MaeI CTAG 6 cut(s) 51, 1298, 1565, 1842, 2033, 2184
MaeII ACGT 3 cut(s) 252, 404, 1785
MalI GATC 6 cut(s) 117, 300, 1011, 1510, 2076, 2113
MboI GATC 6 cut(s) 115, 298, 1009, 1508, 2074, 2111
MhlI GDGCHC 6 cut(s) 926, 1449, 1494, 1564, 1722, 2103
MlsI TGGCCA 1 cut(s) 1961
MluNI TGGCCA 1 cut(s) 1961
MlyI GAGTC 1 cut(s) 6
MmeI TCCRAC 1 cut(s) 1045
Mox20I TGGCCA 1 cut(s) 1961
MroI TCCGGA 1 cut(s) 339
MroNI GCCGGC 1 cut(s) 1343
MroXI GAANNNNTTC 3 cut(s) 687, 758, 1218
MscI TGGCCA 1 cut(s) 1961
MseI TTAA 5 cut(s) 563, 600, 702, 1820, 1898
MslI CAYNNNNRTG 6 cut(s) 585, 864, 1078, 1404, 1608, 2204
Msp20I TGGCCA 1 cut(s) 1961
MspA1I CMGCKG 1 cut(s) 1547
MspI CCGG 2 cut(s) 340, 1344
MspR9I CCNGG 8 cut(s) 93, 161, 191, 837, 936, 1739, 2128, 2166
MvaI CCWGG 8 cut(s) 93, 161, 191, 837, 936, 1739, 2128, 2166
MwoI GCNNNNNNNGC 9 cut(s) 77, 623, 770, 1269, 1550, 1553, 1559, 1863, 1967
NaeI GCCGGC 1 cut(s) 1345
NcoI CCATGG 3 cut(s) 1474, 1881, 2025
NdeI CATATG 1 cut(s) 2215
NdeII GATC 6 cut(s) 115, 298, 1009, 1508, 2074, 2111
NgoMIV GCCGGC 1 cut(s) 1343
NlaIV GGNNCC 4 cut(s) 897, 1183, 1743, 2170
NmuCI GTSAC 4 cut(s) 398, 725, 956, 1781
NspI RCATGY 2 cut(s) 2191, 2203
PaeI GCATGC 1 cut(s) 2191
PagI TCATGA 1 cut(s) 1486
PaqCI CACCTGC 1 cut(s) 2112
PceI AGGCCT 2 cut(s) 796, 2005
PciI ACATGT 1 cut(s) 2199
PdiI GCCGGC 1 cut(s) 1345
PdmI GAANNNNTTC 3 cut(s) 687, 758, 1218
PfeI GAWTC 3 cut(s) 215, 718, 1704
Pfl23II CGTACG 1 cut(s) 405
PkrI GCNGC 8 cut(s) 73, 98, 113, 611, 1546, 1555, 1621, 2140
PleI GAGTC 1 cut(s) 6
PpsI GAGTC 1 cut(s) 6
Ppu21I YACGTR 1 cut(s) 405
PpuMI RGGWCCY 1 cut(s) 567
PscI ACATGT 1 cut(s) 2199
Psp5II RGGWCCY 1 cut(s) 567
Psp6I CCWGG 8 cut(s) 91, 159, 189, 835, 934, 1737, 2126, 2164
PspFI CCCAGC 1 cut(s) 509
PspGI CCWGG 8 cut(s) 91, 159, 189, 835, 934, 1737, 2126, 2164
PspLI CGTACG 1 cut(s) 405
PspN4I GGNNCC 4 cut(s) 897, 1183, 1743, 2170
PspPI GGNCC 4 cut(s) 567, 708, 1741, 2162
PspPPI RGGWCCY 1 cut(s) 567
PstI CTGCAG 2 cut(s) 611, 2074
PstNI CAGNNNCTG 1 cut(s) 77
PvuII CAGCTG 1 cut(s) 1547
RsaI GTAC 6 cut(s) 55, 407, 553, 699, 1127, 2170
RsaNI GTAC 6 cut(s) 54, 406, 552, 698, 1126, 2169
RseI CAYNNNNRTG 6 cut(s) 585, 864, 1078, 1404, 1608, 2204
SaqAI TTAA 5 cut(s) 563, 600, 702, 1820, 1898
SatI GCNGC 8 cut(s) 72, 97, 112, 610, 1545, 1554, 1620, 2139
Sau3AI GATC 6 cut(s) 115, 298, 1009, 1508, 2074, 2111
Sau96I GGNCC 4 cut(s) 567, 708, 1741, 2162
ScaI AGTACT 2 cut(s) 55, 1127
SchI GAGTC 1 cut(s) 6
ScrFI CCNGG 8 cut(s) 93, 161, 191, 837, 936, 1739, 2128, 2166
SduI GDGCHC 6 cut(s) 926, 1449, 1494, 1564, 1722, 2103
SfaNI GCATC 3 cut(s) 795, 1844, 1875
SfcI CTRYAG 4 cut(s) 607, 810, 1713, 2070
SinI GGWCC 3 cut(s) 567, 1741, 2162
SmiMI CAYNNNNRTG 6 cut(s) 585, 864, 1078, 1404, 1608, 2204
SmlI CTYRAG 1 cut(s) 81
SmoI CTYRAG 1 cut(s) 81
SpeI ACTAGT 1 cut(s) 50
SphI GCATGC 1 cut(s) 2191
SseBI AGGCCT 2 cut(s) 796, 2005
SsiI CCGC 6 cut(s) 111, 626, 979, 1016, 1619, 2172
SspI AATATT 2 cut(s) 1627, 1939
SspMI CTAG 6 cut(s) 51, 1298, 1565, 1842, 2033, 2184
StuI AGGCCT 2 cut(s) 796, 2005
StyD4I CCNGG 8 cut(s) 91, 159, 189, 835, 934, 1737, 2126, 2164
StyI CCWWGG 4 cut(s) 475, 1474, 1881, 2025
TaaI ACNGT 6 cut(s) 333, 556, 725, 1024, 1104, 1714
TaiI ACGT 3 cut(s) 255, 407, 1788
TaqI TCGA 3 cut(s) 313, 745, 1606
TatI WGTACW 2 cut(s) 53, 1125
TauI GCSGC 2 cut(s) 114, 1622
TfiI GAWTC 3 cut(s) 215, 718, 1704
Tru1I TTAA 5 cut(s) 563, 600, 702, 1820, 1898
Tru9I TTAA 5 cut(s) 563, 600, 702, 1820, 1898
TscAI CASTG 6 cut(s) 487, 541, 685, 730, 833, 2182
TseFI GTSAC 4 cut(s) 398, 725, 956, 1781
TseI GCWGC 6 cut(s) 71, 96, 609, 1544, 1553, 2138
Tsp45I GTSAC 4 cut(s) 398, 725, 956, 1781
TspGWI ACGGA 3 cut(s) 235, 286, 1174
TspRI CASTG 6 cut(s) 487, 541, 685, 730, 833, 2182
VpaK11BI GGWCC 3 cut(s) 567, 1741, 2162
XapI RAATTY 6 cut(s) 123, 440, 928, 993, 1068, 1200
XbaI TCTAGA 1 cut(s) 1841
XceI RCATGY 2 cut(s) 2191, 2203
XcmI CCANNNNNNNNNTGG 1 cut(s) 833
XmiI GTMKAC 4 cut(s) 249, 1284, 1294, 1458
XmnI GAANNNNTTC 3 cut(s) 687, 758, 1218
XspI CTAG 6 cut(s) 51, 1298, 1565, 1842, 2033, 2184
ZrmI AGTACT 2 cut(s) 55, 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.