pycom15g08290

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000066
Physical Location & Seq
Reverse (-)
5193684 .. 5196695
3012 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g08290.2

Sequence Viewer

Length: 2511 bp
ATGACTACCACGAGTCTGATGAACCTGCAAAACGGCAGAAAATCTTCAAAGAGCTCAGCGGGCACCAGTGTGGTGCCGAACACACAAACATTAATGCTCATGCAGCTCACCTTAATAGAGCTACTGCTACTAGCAGCAGCCACTTCATCCCAAACCACACAATTAATCCCTCCTCCTCCGTTGTCTCCGCCCCTCGATCAAGTTGGAGTCAAGCCGGGTTGCCAAGACAAATGTGGGAATGTTAGCATCCCATATCCATTTGGTACAACCCCAGATTGTTACTACAGCGAGGATTTCCTTATTACTTGCAATGACACACACTACGACCCTCCAAAGCCATTTCTTACAGAAAGTAGCATTGAGGTCACAAACATATCTGTCGACGGGAAGCTGCATATCCTACAATATATAGCCAAAGATTGTTACCACAAATCAGGTGTGTCCTTGGTAAGCAACACTCCTTATCTCCAACTGTCCAAATTCTTCATATCTGACACCGACAACGTGTTTGTGGTGGTTGGTTGCGACACCTCCACTGAAATTGTGGTTTTCCAAGGAAAAGAATATGGATACACAGGTGGGTGCATTACAAAATGTGACAGCATTAATTTTGTTGCCAATTATTCGTGCTCCGGTGTAGGCTGTTGCCAAACATCGATTGCTAAAGATACGAGTTATTATGACGTAAGGGTGAATAGTTTTAAGAATCACACGGATGTGTGGTACTTCAATCCCTGCAGCTATGCCTTTATTGTCGAAGAGAGCAAGTTCAACTTCTCTTCGAATATGCTGACCGATTTGATCAACGTGGAAGTGCTTCCGGTGGTTCTAGATTGGTCAATTGGGCATGAGACATGCAGCAAGGTTGTTAGAAACAAACGAGTGATGAATTACGCATGTCATGGGGACGAAACATGTATCGATGTCAAGAATGGTCCGGGTTATCGCTGCAAGTGCCCAGATGGTTACCGAGGGAATCCATACCTTGAGGGATGCCATGGTAATTTTCCTAGCTTAAGCTTGTTTTTTGTATATCTTTCAATCTTTGTAATGTCATTTGCTTTCAAAACTTTAGTACTACTACTAATTAATACAACTTGCTTTCTGAAAATTATGGGTCATGTTATTTTTGCAGACGTTAATGAATGTGAGGATGCAAACCTCAATAAGTGCGAACAGAAATGTACTAATACAGAAGGAGGCTATGCTTGTTCTTGCCGCAAGGGGTACCATAGCAAGGGTAAAGTAGACGGAGAAGGTTGCACCCCTAATCCAACATTACTTGTTCAGATTATCGTTGGAATCGGGGTTGGCCTCATAGCGTTGCTAATGGGAAGCTCTTGGTTGTATTTGGGCTACAAAAGATGGAAGCTCATAAAGCTGAAGGAGAAGTTCTTCAGGCAAAATGGAGGACTAATGCTACAACAACAACTTTCAGAACGACAGGGGTCCACTCACGAAACTGCCAAAATCTTCACAGCAGAAGAACTTGAGAAGGCCACAAATAATTACAATGAGTCTAAAATCATTGGCAGAGGAGGTTTTGGTACAGTGTACAAAGGAATTTTAGTAGACGGCCGAGTAATTGCTATCAAGAAGTCCAAAATGGTGGATCAAAGCCAAATAGAGCAATTCATAAATGAGGTACTGGTGCTTTCACAGATCAACCATAGGAATGTGGTTAAGCTTTTAGGTTGTTGTTTCGAGACACAAGTTCCACTGCTAGTTTATGAGTATGTAACAAATGGAACACTCTTTGACTACATCCACAATACAACCAAGGAATCCAAACCAAACACCTCTTGGGAAATCCATCTAAAGATAGGTTCAGAAACAGCCGGAGTGCTGTCATACTTGCACTCTGCGGCTTCTGTTCCTATCATTCACAGAGATGTCAAGTCTACAAACATACTCTTAGATGAAAATCTCACAGCCAAAGTGTCTGACTTTGGAGCCTCGAGATTGGTTCCGTTAGGCCAAGTTCAGTTGTCCACAATGGTGCAAGGAACTATGGGGTATTTAGACCCCGAATACTTGCAGACAAGCCAATTGACTGAGAAAAGCGATGTCTATAGTTTCGGGGTCGTCCTCGTAGAGCTATTGACAAGAAAGAAGGCGGTTTTCTTTGACAAACCGGGGGAACAGATAAATTTGGTTATGTATTTTCTTTCAGCATTGAAAGATGACCGCTTGCTGCAAGTTCTAGATGATTGGATTGTGACTGAGGCAAACATTGAGCAGCTTAAAGAAGTTTCTATGCTTGCAAAGAGGTGTTTGAGAGTAAAAGGAGAAGAAAGACCAACGATGCAGGAAGTTGCAATGGAGCTAGAGGGACTGAGAAGGATGGTATTGCATCCGTGGGTGAATGATGAATCCATTTCAGAAGAGACTCAGCACTTGCTTGGTGGAATATCAATGGAAACTATTAGTTATGAAGGAGTTGGCGATTCAGGCTTAGGCTATGATACTATGAGGAACAACGTTGCATTACCAGTTAATGGTGGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase WAK

Protein Analysis

837

Amino Acids

92.92

Weight (kDa)

5.8

Isoelectric Point (pI)

33.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 33
Acc65I GGTACC 1 cut(s) 1227
AccB1I GGYRCC 3 cut(s) 62, 73, 1227
AccB7I CCANNNNNTGG 1 cut(s) 2498
AccI GTMKAC 4 cut(s) 381, 1248, 1572, 1901
AciI CCGC 6 cut(s) 59, 188, 1219, 1865, 2117, 2188
AclI AACGTT 1 cut(s) 2481
AclWI GGATC 1 cut(s) 1620
AcoI YGGCCR 1 cut(s) 1576
AcsI RAATTY 3 cut(s) 479, 1563, 2149
AcuI CTGAAG 2 cut(s) 1381, 1403
AfaI GTAC 8 cut(s) 265, 725, 1077, 1186, 1229, 1549, 1556, 1647
AfiI CCNNNNNNNGG 2 cut(s) 1237, 2498
AflII CTTAAG 1 cut(s) 1015
AflIII ACRYGT 2 cut(s) 504, 914
AgsI TTSAA 6 cut(s) 48, 730, 772, 1041, 1066, 2179
AleI CACNNNNGTG 3 cut(s) 68, 716, 1997
Alw21I GWGCWC 2 cut(s) 56, 632
Alw26I GTCTC 4 cut(s) 189, 845, 1700, 2381
AlwI GGATC 1 cut(s) 1620
Ama87I CYCGRG 1 cut(s) 1957
AoxI GGCC 4 cut(s) 1312, 1497, 1576, 1975
ApeKI GCWGC 9 cut(s) 103, 134, 137, 391, 738, 858, 948, 2194, 2239
ApoI RAATTY 3 cut(s) 479, 1563, 2149
ArsI GACNNNNNNTTYG 2 cut(s) 491, 523
AseI ATTAAT 4 cut(s) 92, 164, 606, 1089
Asp700I GAANNNNTTC 3 cut(s) 43, 816, 1394
Asp718I GGTACC 1 cut(s) 1227
AspS9I GGNCC 2 cut(s) 935, 1449
AsuC2I CCSGG 3 cut(s) 216, 939, 2136
AsuHPI GGTGA 3 cut(s) 100, 703, 2374
AsuII TTCGAA 1 cut(s) 782
AvaI CYCGRG 1 cut(s) 1957
AvaII GGWCC 2 cut(s) 935, 1449
BaeGI GKGCMC 2 cut(s) 65, 959
BaeI ACNNNNGTAYC 2 cut(s) 562, 595
BanI GGYRCC 3 cut(s) 62, 73, 1227
BanII GRGCYC 1 cut(s) 56
BauI CACGAG 1 cut(s) 10
Bbv12I GWGCWC 2 cut(s) 56, 632
BbvI GCAGC 9 cut(s) 115, 146, 149, 378, 750, 870, 935, 2181, 2251
BccI CCATC 4 cut(s) 956, 1359, 1821, 2338
BceAI ACGGC 2 cut(s) 49, 1591
BciVI GTATCC 1 cut(s) 563
BclI TGATCA 1 cut(s) 801
BcnI CCSGG 3 cut(s) 216, 939, 2136
BcoDI GTCTC 4 cut(s) 189, 845, 1700, 2381
BfaI CTAG 6 cut(s) 131, 830, 1011, 1724, 2204, 2327
BfmI CTRYAG 3 cut(s) 283, 736, 2071
BfrI CTTAAG 1 cut(s) 1015
BfuAI ACCTGC 1 cut(s) 33
BfuI GTATCC 1 cut(s) 563
BlpI GCTNAGC 1 cut(s) 55
BmcAI AGTACT 1 cut(s) 1077
Bme1390I CCNGG 3 cut(s) 216, 939, 2136
Bme18I GGWCC 2 cut(s) 935, 1449
BmeT110I CYCGRG 1 cut(s) 1957
BmgT120I GGNCC 2 cut(s) 935, 1449
BmiI GGNNCC 6 cut(s) 64, 75, 1229, 1450, 1954, 1968
BmrFI CCNGG 3 cut(s) 216, 939, 2136
BmsI GCATC 5 cut(s) 255, 983, 1144, 2295, 2362
BoxI GACNNNNGTC 1 cut(s) 1447
Bpu10I CCTNAGC 1 cut(s) 2455
Bpu1102I GCTNAGC 1 cut(s) 55
Bpu14I TTCGAA 1 cut(s) 782
BpuEI CTTGAG 2 cut(s) 1007, 1511
BpuMI CCSGG 3 cut(s) 216, 939, 2136
Bsa29I ATCGAT 2 cut(s) 656, 921
BsaBI GATNNNNATC 1 cut(s) 1923
BsaJI CCNNGG 7 cut(s) 444, 553, 970, 997, 1779, 2135, 2357
BsaWI WCCGGW 2 cut(s) 632, 820
Bsc4I CCNNNNNNNGG 2 cut(s) 1237, 2498
Bse1I ACTGG 3 cut(s) 66, 1653, 2492
Bse3DI GCAATG 2 cut(s) 316, 2325
Bse8I GATNNNNATC 1 cut(s) 1923
BseCI ATCGAT 2 cut(s) 656, 921
BseDI CCNNGG 7 cut(s) 444, 553, 970, 997, 1779, 2135, 2357
BseGI GGATG 8 cut(s) 146, 246, 721, 998, 1159, 1764, 2349, 2353
BseJI GATNNNNATC 1 cut(s) 1923
BseLI CCNNNNNNNGG 2 cut(s) 1237, 2498
BseMI GCAATG 2 cut(s) 316, 2325
BseMII CTCAG 5 cut(s) 69, 2046, 2214, 2327, 2405
BseNI ACTGG 3 cut(s) 66, 1653, 2492
BseRI GAGGAG 3 cut(s) 162, 165, 1551
BseSI GKGCMC 2 cut(s) 65, 959
BseX3I CGGCCG 1 cut(s) 1576
BseXI GCAGC 9 cut(s) 115, 146, 149, 378, 750, 870, 935, 2181, 2251
Bsh1285I CGRYCG 1 cut(s) 1579
BshFI GGCC 4 cut(s) 1314, 1499, 1578, 1977
BshNI GGYRCC 3 cut(s) 62, 73, 1227
BshVI ATCGAT 2 cut(s) 656, 921
BsiEI CGRYCG 1 cut(s) 1579
BsiHKAI GWGCWC 2 cut(s) 56, 632
BsiHKCI CYCGRG 1 cut(s) 1957
BsiSI CCGG 6 cut(s) 215, 633, 821, 938, 1839, 2135
BslFI GGGAC 2 cut(s) 920, 2346
BslI CCNNNNNNNGG 2 cut(s) 1237, 2498
BsmAI GTCTC 4 cut(s) 189, 845, 1700, 2381
BsmFI GGGAC 2 cut(s) 920, 2346
BsnI GGCC 4 cut(s) 1314, 1499, 1578, 1977
BsoBI CYCGRG 1 cut(s) 1957
Bsp119I TTCGAA 1 cut(s) 782
Bsp1286I GDGCHC 4 cut(s) 56, 65, 632, 959
Bsp1407I TGTACA 1 cut(s) 1554
Bsp143I GATC 4 cut(s) 196, 801, 1612, 1662
Bsp1720I GCTNAGC 1 cut(s) 55
Bsp19I CCATGG 1 cut(s) 997
BspACI CCGC 6 cut(s) 59, 188, 1219, 1865, 2117, 2188
BspANI GGCC 4 cut(s) 1314, 1499, 1578, 1977
BspCNI CTCAG 5 cut(s) 68, 2047, 2215, 2328, 2404
BspDI ATCGAT 2 cut(s) 656, 921
BspLI GGNNCC 6 cut(s) 64, 75, 1229, 1450, 1954, 1968
BspMAI CTGCAG 1 cut(s) 740
BspMI ACCTGC 1 cut(s) 33
BspPI GGATC 1 cut(s) 1620
BspT104I TTCGAA 1 cut(s) 782
BspT107I GGYRCC 3 cut(s) 62, 73, 1227
BspTI CTTAAG 1 cut(s) 1015
BsrDI GCAATG 2 cut(s) 316, 2325
BsrGI TGTACA 1 cut(s) 1554
BsrI ACTGG 3 cut(s) 66, 1653, 2492
BssECI CCNNGG 7 cut(s) 444, 553, 970, 997, 1779, 2135, 2357
BssMI GATC 4 cut(s) 196, 801, 1612, 1662
BssSI CACGAG 1 cut(s) 10
BssT1I CCWWGG 4 cut(s) 444, 553, 997, 1779
Bst2BI CACGAG 1 cut(s) 10
Bst4CI ACNGT 2 cut(s) 474, 1552
Bst6I CTCTTC 3 cut(s) 753, 784, 2379
BstAFI CTTAAG 1 cut(s) 1015
BstAUI TGTACA 1 cut(s) 1554
BstBI TTCGAA 1 cut(s) 782
BstC8I GCNNGC 3 cut(s) 61, 2192, 2262
BstDEI CTNAG 7 cut(s) 55, 1915, 2055, 2223, 2336, 2391, 2455
BstDSI CCRYGG 2 cut(s) 997, 2357
BstEII GGTNACC 1 cut(s) 965
BstF5I GGATG 8 cut(s) 146, 246, 721, 998, 1159, 1764, 2349, 2353
BstKTI GATC 4 cut(s) 199, 804, 1615, 1665
BstMAI GTCTC 4 cut(s) 189, 845, 1700, 2381
BstMBI GATC 4 cut(s) 196, 801, 1612, 1662
BstMCI CGRYCG 1 cut(s) 1579
BstMWI GCNNNNNNNGC 5 cut(s) 60, 103, 954, 1378, 2451
BstNSI RCATGY 3 cut(s) 858, 900, 918
BstPAI GACNNNNGTC 1 cut(s) 1447
BstPI GGTNACC 1 cut(s) 965
BstSCI CCNGG 3 cut(s) 214, 937, 2134
BstSFI CTRYAG 3 cut(s) 283, 736, 2071
BstSLI GKGCMC 2 cut(s) 65, 959
BstV1I GCAGC 9 cut(s) 115, 146, 149, 378, 750, 870, 935, 2181, 2251
BstXI CCANNNNNNTGG 1 cut(s) 1609
BstZI CGGCCG 1 cut(s) 1576
Bsu15I ATCGAT 2 cut(s) 656, 921
BsuI GTATCC 1 cut(s) 563
BsuRI GGCC 4 cut(s) 1314, 1499, 1578, 1977
BsuTUI ATCGAT 2 cut(s) 656, 921
BtgI CCRYGG 2 cut(s) 997, 2357
BtgZI GCGATG 1 cut(s) 2079
BtsCI GGATG 8 cut(s) 146, 246, 721, 998, 1159, 1764, 2349, 2353
BtsI GCAGTG 1 cut(s) 1718
BtsIMutI CAGTG 4 cut(s) 73, 534, 1557, 1718
BveI ACCTGC 1 cut(s) 33
Cac8I GCNNGC 3 cut(s) 61, 2192, 2262
Cfr13I GGNCC 2 cut(s) 935, 1449
ClaI ATCGAT 2 cut(s) 656, 921
Csp6I GTAC 8 cut(s) 264, 724, 1076, 1185, 1228, 1548, 1555, 1646
CspCI CAANNNNNGTGG 2 cut(s) 523, 558
CviAII CATG 8 cut(s) 100, 848, 855, 897, 902, 915, 998, 1121
CviQI GTAC 8 cut(s) 264, 724, 1076, 1185, 1228, 1548, 1555, 1646
DdeI CTNAG 7 cut(s) 55, 1915, 2055, 2223, 2336, 2391, 2455
DpnI GATC 4 cut(s) 198, 803, 1614, 1664
DpnII GATC 4 cut(s) 196, 801, 1612, 1662
EaeI YGGCCR 1 cut(s) 1576
EagI CGGCCG 1 cut(s) 1576
Eam1104I CTCTTC 3 cut(s) 753, 784, 2379
EarI CTCTTC 3 cut(s) 753, 784, 2379
EciI GGCGGA 1 cut(s) 177
Ecl136II GAGCTC 1 cut(s) 54
EclXI CGGCCG 1 cut(s) 1576
Eco130I CCWWGG 4 cut(s) 444, 553, 997, 1779
Eco24I GRGCYC 1 cut(s) 56
Eco47I GGWCC 2 cut(s) 935, 1449
Eco52I CGGCCG 1 cut(s) 1576
Eco53kI GAGCTC 1 cut(s) 54
Eco57I CTGAAG 2 cut(s) 1381, 1403
Eco88I CYCGRG 1 cut(s) 1957
Eco91I GGTNACC 1 cut(s) 965
EcoICRI GAGCTC 1 cut(s) 54
EcoO65I GGTNACC 1 cut(s) 965
EcoT14I CCWWGG 4 cut(s) 444, 553, 997, 1779
EcoT38I GRGCYC 1 cut(s) 56
ErhI CCWWGG 4 cut(s) 444, 553, 997, 1779
FaeI CATG 8 cut(s) 103, 851, 858, 900, 905, 918, 1001, 1124
FalI AAGNNNNNCTT 2 cut(s) 758, 790
FaqI GGGAC 2 cut(s) 920, 2346
FatI CATG 8 cut(s) 99, 847, 854, 896, 901, 914, 997, 1120
FauI CCCGC 1 cut(s) 52
FbaI TGATCA 1 cut(s) 801
FblI GTMKAC 4 cut(s) 381, 1248, 1572, 1901
FokI GGATG 8 cut(s) 133, 233, 728, 1005, 1166, 1751, 2340, 2356
FriOI GRGCYC 1 cut(s) 56
FspBI CTAG 6 cut(s) 131, 830, 1011, 1724, 2204, 2327
HaeIII GGCC 4 cut(s) 1314, 1499, 1578, 1977
HapII CCGG 6 cut(s) 215, 633, 821, 938, 1839, 2135
Hin1II CATG 8 cut(s) 103, 851, 858, 900, 905, 918, 1001, 1124
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HindIII AAGCTT 2 cut(s) 1018, 1685
HpaII CCGG 6 cut(s) 215, 633, 821, 938, 1839, 2135
HphI GGTGA 3 cut(s) 100, 703, 2374
Hpy166II GTNNAC 7 cut(s) 382, 1249, 1452, 1555, 1573, 1902, 1992
Hpy188I TCNGA 8 cut(s) 18, 493, 1107, 1290, 1438, 1831, 1945, 2383
Hpy188III TCNNGA 7 cut(s) 830, 928, 1457, 1594, 1705, 1959, 2204
Hpy8I GTNNAC 7 cut(s) 382, 1249, 1452, 1555, 1573, 1902, 1992
Hpy99I CGWCG 1 cut(s) 386
HpyAV CCTTC 7 cut(s) 1190, 1250, 1378, 1489, 2107, 2334, 2429
HpyCH4III ACNGT 2 cut(s) 474, 1552
HpyCH4IV ACGT 5 cut(s) 504, 684, 807, 1137, 2481
HpyF10VI GCNNNNNNNGC 5 cut(s) 60, 103, 954, 1378, 2451
HpyF3I CTNAG 7 cut(s) 55, 1915, 2055, 2223, 2336, 2391, 2455
HpySE526I ACGT 5 cut(s) 504, 684, 807, 1137, 2481
Hsp92II CATG 8 cut(s) 103, 851, 858, 900, 905, 918, 1001, 1124
KpnI GGTACC 1 cut(s) 1231
Ksp22I TGATCA 1 cut(s) 801
Kzo9I GATC 4 cut(s) 196, 801, 1612, 1662
LmnI GCTCC 3 cut(s) 635, 1952, 2323
Lsp1109I GCAGC 9 cut(s) 115, 146, 149, 378, 750, 870, 935, 2181, 2251
LweI GCATC 5 cut(s) 255, 983, 1144, 2295, 2362
MaeI CTAG 6 cut(s) 131, 830, 1011, 1724, 2204, 2327
MaeII ACGT 5 cut(s) 504, 684, 807, 1137, 2481
MaeIII GTNAC 7 cut(s) 278, 364, 422, 596, 965, 1738, 2218
MalI GATC 4 cut(s) 198, 803, 1614, 1664
MboI GATC 4 cut(s) 196, 801, 1612, 1662
MboII GAAGA 9 cut(s) 36, 475, 770, 771, 1387, 1465, 1496, 2303, 2396
MfeI CAATTG 2 cut(s) 840, 2048
MhlI GDGCHC 4 cut(s) 56, 65, 632, 959
MlyI GAGTC 4 cut(s) 22, 216, 1526, 2383
MmeI TCCRAC 4 cut(s) 184, 493, 1279, 1298
MroXI GAANNNNTTC 3 cut(s) 43, 816, 1394
MslI CAYNNNNRTG 6 cut(s) 68, 714, 716, 1674, 1890, 1997
MspA1I CMGCKG 1 cut(s) 59
MspCI CTTAAG 1 cut(s) 1015
MspI CCGG 6 cut(s) 215, 633, 821, 938, 1839, 2135
MspR9I CCNGG 3 cut(s) 216, 939, 2136
MunI CAATTG 2 cut(s) 840, 2048
MwoI GCNNNNNNNGC 5 cut(s) 60, 103, 954, 1378, 2451
NciI CCSGG 3 cut(s) 216, 939, 2136
NcoI CCATGG 1 cut(s) 997
NdeII GATC 4 cut(s) 196, 801, 1612, 1662
NlaIII CATG 8 cut(s) 103, 851, 858, 900, 905, 918, 1001, 1124
NlaIV GGNNCC 6 cut(s) 64, 75, 1229, 1450, 1954, 1968
NmeAIII GCCGAG 1 cut(s) 1604
NmuCI GTSAC 3 cut(s) 364, 596, 2218
NspI RCATGY 3 cut(s) 858, 900, 918
NspV TTCGAA 1 cut(s) 782
OliI CACNNNNGTG 3 cut(s) 68, 716, 1997
PaeR7I CTCGAG 1 cut(s) 1957
PciI ACATGT 1 cut(s) 914
PdmI GAANNNNTTC 3 cut(s) 43, 816, 1394
PfeI GAWTC 6 cut(s) 706, 976, 1302, 1784, 2372, 2447
PflMI CCANNNNNTGG 1 cut(s) 2498
PleI GAGTC 4 cut(s) 21, 215, 1525, 2383
PpsI GAGTC 4 cut(s) 21, 215, 1525, 2383
PscI ACATGT 1 cut(s) 914
PshAI GACNNNNGTC 1 cut(s) 1447
PshBI ATTAAT 4 cut(s) 92, 164, 606, 1089
Psp124BI GAGCTC 1 cut(s) 56
Psp1406I AACGTT 1 cut(s) 2481
PspEI GGTNACC 1 cut(s) 965
PspN4I GGNNCC 6 cut(s) 64, 75, 1229, 1450, 1954, 1968
PspPI GGNCC 2 cut(s) 935, 1449
PstI CTGCAG 1 cut(s) 740
RsaI GTAC 8 cut(s) 265, 725, 1077, 1186, 1229, 1549, 1556, 1647
RsaNI GTAC 8 cut(s) 264, 724, 1076, 1185, 1228, 1548, 1555, 1646
RseI CAYNNNNRTG 6 cut(s) 68, 714, 716, 1674, 1890, 1997
SacI GAGCTC 1 cut(s) 56
SalI GTCGAC 1 cut(s) 380
Sau3AI GATC 4 cut(s) 196, 801, 1612, 1662
Sau96I GGNCC 2 cut(s) 935, 1449
ScaI AGTACT 1 cut(s) 1077
SchI GAGTC 4 cut(s) 22, 216, 1526, 2383
ScrFI CCNGG 3 cut(s) 216, 939, 2136
SduI GDGCHC 4 cut(s) 56, 65, 632, 959
SfaNI GCATC 5 cut(s) 255, 983, 1144, 2295, 2362
SfcI CTRYAG 3 cut(s) 283, 736, 2071
Sfr274I CTCGAG 1 cut(s) 1957
SfuI TTCGAA 1 cut(s) 782
SinI GGWCC 2 cut(s) 935, 1449
SlaI CTCGAG 1 cut(s) 1957
SmiMI CAYNNNNRTG 6 cut(s) 68, 714, 716, 1674, 1890, 1997
SmlI CTYRAG 4 cut(s) 986, 1015, 1490, 1957
SmoI CTYRAG 4 cut(s) 986, 1015, 1490, 1957
SsiI CCGC 6 cut(s) 59, 188, 1219, 1865, 2117, 2188
SspMI CTAG 6 cut(s) 131, 830, 1011, 1724, 2204, 2327
SstI GAGCTC 1 cut(s) 56
StyD4I CCNGG 3 cut(s) 214, 937, 2134
StyI CCWWGG 4 cut(s) 444, 553, 997, 1779
TaaI ACNGT 2 cut(s) 474, 1552
TaiI ACGT 5 cut(s) 507, 687, 810, 1140, 2484
TaqI TCGA 8 cut(s) 195, 381, 656, 756, 782, 921, 1704, 1958
TaqII GACCGA 1 cut(s) 809
TatI WGTACW 3 cut(s) 1075, 1184, 1554
TauI GCSGC 2 cut(s) 1221, 1868
TfiI GAWTC 6 cut(s) 706, 976, 1302, 1784, 2372, 2447
TscAI CASTG 4 cut(s) 73, 541, 1557, 1725
TseFI GTSAC 3 cut(s) 364, 596, 2218
TseI GCWGC 9 cut(s) 103, 134, 137, 391, 738, 858, 948, 2194, 2239
Tsp45I GTSAC 3 cut(s) 364, 596, 2218
TspDTI ATGAA 9 cut(s) 35, 135, 475, 902, 1158, 1624, 1935, 2385, 2448
TspGWI ACGGA 5 cut(s) 168, 728, 1266, 1959, 2346
TspRI CASTG 4 cut(s) 73, 541, 1557, 1725
Van91I CCANNNNNTGG 1 cut(s) 2498
Vha464I CTTAAG 1 cut(s) 1015
VpaK11BI GGWCC 2 cut(s) 935, 1449
VspI ATTAAT 4 cut(s) 92, 164, 606, 1089
XapI RAATTY 3 cut(s) 479, 1563, 2149
XbaI TCTAGA 2 cut(s) 829, 2203
XceI RCATGY 3 cut(s) 858, 900, 918
XcmI CCANNNNNNNNNTGG 3 cut(s) 230, 541, 1800
XhoI CTCGAG 1 cut(s) 1957
XmiI GTMKAC 4 cut(s) 381, 1248, 1572, 1901
XmnI GAANNNNTTC 3 cut(s) 43, 816, 1394
XspI CTAG 6 cut(s) 131, 830, 1011, 1724, 2204, 2327
ZrmI AGTACT 1 cut(s) 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.