Rroxscaffold_1G00026270

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000066
Physical Location & Seq
Reverse (-)
33074566 .. 33077803
3238 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00026270.1

Sequence Viewer

Length: 2202 bp
ATGGCTGTGCACGGGAGGATGCTAGTTATGAAACTGATCATAGCGGTGGCCATAGCAGCAGCAGCAGCTGATCGAATCCCACCTCGTCAAGCGCGACCTAACTGCCCCGACCACTGTGGTAATCTCACAATCCCATATCCTTTCGGCATTGGGAAGGGTTGTTTCTTGCGGAATGGGTTCAACCTCACTTGTAACCAAACCGCCAAACCCCCAACAACACTCTGGGGTGATGGTAGTTTCACGGTTACTAACATATTGCTTGCTGAAGGTGAGTTGGAGGTTATGAATTACATATCCCATGATTGTTATGATAAGCAGGGTAATCAAACAAAAGACATGTACAATTTCCCTAGTCTCCGGTTGCAACCTCCCTTCACCATCTCCGGGACCAGAAACAAGTTTATTGCCGTTGGCTGCGACAATTGGGCAATTTTCAAAGGGTACCGCCGTTACCCTCATGACGAAGATAGGTACATCACCGGATGCATAACTGTATGTAACAATCTTGACAGTGTAGTTAACGGTTCGTGCTCTGGCATTGGGTGTTGCCAAACTGCCATCCCTAGCGGACTCAAAAATGGCACCGTCAAATTGGATAGCTATTCTCAACACACGGCGGTGTGGGACAACAATCCATGCAGCTACGCCTTCATTGTGGAAGAAGGCAGGTTCACATTCAACCCTAAAACAAGTTTTGAAGAACTCAACAACACTAAAAAACTTCCCGTGATTATTAACTGGGCAATTCATGAAGGGTCGTGTGCACTAGCTCAAAACAGCTCTGGTTATGCTTGCAAAACAAATAGCAGCTGTGTCAATCGGACCGTCGATTACCAGACTGAACCGACTGGTTATTATTGCCAGTGCTTGCCAGGCTTCGAAGGGAACCCATATCTTCCAGATGGTTGCCAAGATATTGACGAGTGCATGGCTCCATCAAGTCCCTGCAACAATGGAATATGCCAAAATTCACCTGGAGGTCTCAGTATAGGCTTCTTCATTTTAGTGGTTTTAATTTGGTGGACTTATTGCGGAATGAACAAAAGAAGACTAGTAAGACTCAAAGAAAAGTACTTCGAAGAAAATGGCGGCTTAACATTACAGCAACATCTCTCTAGTCATGGAGGATCTATTGAGACAGTAGAAATCTTTACTGCAGAGGAACTTCAGAAAGCCACAAACAATTACCATGCAGATGAAATCCTTGGTGAAGGAGGCTATGCAACAGTTTACAAAGGAATACTACATGATAAGAATGTTGTTGCCATAAAGAAGCCAAAGATTGGCACTCCAACTCAAAGTGATCAGTTCGTTAATGAGATTATCGTTCTTTCTCAGATAAACCACAGAAATGTAGTAAAACTATTAGGTTGTTGTTTCGAGACCGAAGTGCCTTTACTGGTATATGAGTACATTACTCATGGCACTCTTTTCGAGCATGTATTCAATAAGGGAAAAGGATCCTCGCTTTCATGGGAACTACGATTGAAGATAGCATCAGAAACTGCAGGCGCATTAGCATACTTACACTCAGCTACTTCCACTCCAATCATACATAGAGACGTGAAAACAACGAATATATTATTGGATGACAATTACACAGCCAAAGTGTCTGATTTTGGAGCTTCGCGTTTCATTCCTCTAGATCAAACTCAACTTGCAACACTGGTTCAAGGGACGCTTGGATACTTGGATCCTGAATACTTTCATTCAAACCAACTAACTGAAAAGAGTGACGTCTATAGTTTTGGAGTTGTCCTAGCGGAGCTACTAACAAGTAGAGTTGCACTTTCTTTTGCCAGGCCTGACAAGGAGAGATGCCTAGCAAGCTTCTTTGTTTCTTCAATAGAAAACGAATGCTTGATTCAAATTCTTGATGCTGATATAGTGAATGAGGAGAACATAGAGACGGTAGAGCAAGTGGCGATTCTGGCAAAAAGATGTTTGATGATAAAAAGTGAGGAAAGGCCTACGATGAAAGACGTTGCCATGGAGTTAGAAGGGATGAGAATTATGGCAAAGCATCCATGGAAAAATGGCAATTCCGGCTTTGAGGAGACTGAGTACTTGCTCGGGTCACCAAATTCAGCGGCTTACGTGATGGATATTGGAGGTGGAGGTGCCGGTGGTTCGAGTGTTACAAATACTGGGTATGACAGCATGCAAATTCAAATGTTAATGCCCTACGATGATGGGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

733

Amino Acids

80.95

Weight (kDa)

5.48

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 95 1.9e-12 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 396 - 662 1.5e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 398 - 666 3e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1740
Acc36I ACCTGC 1 cut(s) 657
Acc65I GGTACC 1 cut(s) 441
AccB1I GGYRCC 3 cut(s) 441, 581, 2120
AccB7I CCANNNNNTGG 1 cut(s) 1283
AccII CGCG 2 cut(s) 94, 1630
AclWI GGATC 5 cut(s) 1135, 1455, 1468, 1688, 1701
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 4 cut(s) 967, 1869, 2083, 2166
AcuI CTGAAG 2 cut(s) 285, 1151
AcyI GRCGYC 1 cut(s) 1737
AfaI GTAC 6 cut(s) 341, 443, 473, 1073, 1412, 2066
AfiI CCNNNNNNNGG 2 cut(s) 384, 1283
AflIII ACRYGT 1 cut(s) 336
AhlI ACTAGT 1 cut(s) 1051
AjiI CACGTC 1 cut(s) 1564
AjnI CCWGG 3 cut(s) 871, 973, 1799
AjuI GAANNNNNNNTTGG 2 cut(s) 1568, 1600
AleI CACNNNNGTG 1 cut(s) 617
Alw21I GWGCWC 3 cut(s) 12, 533, 766
Alw26I GTCTC 7 cut(s) 359, 986, 1130, 1376, 1554, 1901, 2051
Alw44I GTGCAC 2 cut(s) 8, 762
AlwI GGATC 5 cut(s) 1135, 1455, 1468, 1688, 1701
AlwNI CAGNNNCTG 2 cut(s) 68, 1505
Ama87I CYCGRG 1 cut(s) 2072
AoxI GGCC 3 cut(s) 48, 1802, 1967
ApaLI GTGCAC 2 cut(s) 8, 762
ApeKI GCWGC 7 cut(s) 56, 59, 62, 65, 414, 639, 807
ApoI RAATTY 4 cut(s) 967, 1869, 2083, 2166
Asp700I GAANNNNTTC 2 cut(s) 176, 1704
Asp718I GGTACC 1 cut(s) 441
AspLEI GCGC 2 cut(s) 94, 1514
AspS9I GGNCC 2 cut(s) 387, 822
AsuC2I CCSGG 1 cut(s) 385
AsuHPI GGTGA 7 cut(s) 239, 281, 367, 469, 963, 1220, 2070
AsuII TTCGAA 2 cut(s) 879, 1077
AvaI CYCGRG 1 cut(s) 2072
AvaII GGWCC 2 cut(s) 387, 822
BaeGI GKGCMC 2 cut(s) 12, 766
BaeI ACNNNNGTAYC 2 cut(s) 433, 466
BalI TGGCCA 1 cut(s) 50
BamHI GGATCC 2 cut(s) 1460, 1693
BanI GGYRCC 3 cut(s) 441, 581, 2120
BarI GAAGNNNNNNTAC 2 cut(s) 1380, 1412
BbsI GAAGAC 1 cut(s) 1054
Bbv12I GWGCWC 3 cut(s) 12, 533, 766
BbvI GCAGC 7 cut(s) 68, 71, 74, 77, 401, 651, 819
BccI CCATC 7 cut(s) 224, 386, 566, 896, 943, 2095, 2186
BceAI ACGGC 3 cut(s) 392, 432, 630
BcgI CGANNNNNNTGC 4 cut(s) 84, 118, 1414, 1448
BciT130I CCWGG 3 cut(s) 873, 975, 1801
BciVI GTATCC 1 cut(s) 1679
BclI TGATCA 2 cut(s) 36, 1303
BcnI CCSGG 1 cut(s) 385
BcoDI GTCTC 7 cut(s) 359, 986, 1130, 1376, 1554, 1901, 2051
BcuI ACTAGT 1 cut(s) 1051
BfaI CTAG 9 cut(s) 23, 351, 564, 767, 1052, 1116, 1643, 1760, 1823
BfmI CTRYAG 3 cut(s) 1155, 1506, 1741
BfuAI ACCTGC 1 cut(s) 657
BfuI GTATCC 1 cut(s) 1679
BisI GCNGC 9 cut(s) 57, 60, 63, 66, 415, 640, 808, 1090, 2091
BlsI GCNGC 9 cut(s) 58, 61, 64, 67, 416, 641, 809, 1091, 2092
BmcAI AGTACT 2 cut(s) 1073, 2066
Bme1390I CCNGG 4 cut(s) 385, 873, 975, 1801
Bme18I GGWCC 2 cut(s) 387, 822
BmeT110I CYCGRG 1 cut(s) 2072
BmgBI CACGTC 1 cut(s) 1564
BmgT120I GGNCC 2 cut(s) 387, 822
BmiI GGNNCC 8 cut(s) 388, 443, 583, 887, 933, 1462, 1695, 2122
BmrFI CCNGG 4 cut(s) 385, 873, 975, 1801
BmrI ACTGGG 2 cut(s) 748, 2157
BmsI GCATC 6 cut(s) 9, 473, 1505, 1808, 1867, 2032
BmuI ACTGGG 2 cut(s) 748, 2157
BpiI GAAGAC 1 cut(s) 1054
BpmI CTGGAG 1 cut(s) 996
Bpu14I TTCGAA 2 cut(s) 879, 1077
BpuMI CCSGG 1 cut(s) 385
BsaAI YACGTR 1 cut(s) 2098
BsaHI GRCGYC 1 cut(s) 1737
BsaI GGTCTC 2 cut(s) 986, 1376
BsaJI CCNNGG 3 cut(s) 1204, 1989, 2027
BsaWI WCCGGW 2 cut(s) 357, 479
BsaXI ACNNNNNCTCC 4 cut(s) 1528, 1558, 2048, 2078
Bsc4I CCNNNNNNNGG 2 cut(s) 384, 1283
Bse118I RCCGGY 1 cut(s) 2123
Bse1I ACTGG 6 cut(s) 743, 853, 862, 1404, 1671, 2152
BseBI CCWGG 3 cut(s) 873, 975, 1801
BseDI CCNNGG 3 cut(s) 1204, 1989, 2027
BseGI GGATG 6 cut(s) 24, 488, 558, 1594, 2010, 2023
BseLI CCNNNNNNNGG 2 cut(s) 384, 1283
BseMII CTCAG 4 cut(s) 997, 1349, 1545, 2052
BseNI ACTGG 6 cut(s) 743, 853, 862, 1404, 1671, 2152
BseRI GAGGAG 2 cut(s) 1910, 2069
BseSI GKGCMC 2 cut(s) 12, 766
BseXI GCAGC 7 cut(s) 68, 71, 74, 77, 401, 651, 819
Bsh1236I CGCG 2 cut(s) 94, 1630
BshFI GGCC 3 cut(s) 50, 1804, 1969
BshNI GGYRCC 3 cut(s) 441, 581, 2120
BsiHKAI GWGCWC 3 cut(s) 12, 533, 766
BsiHKCI CYCGRG 1 cut(s) 2072
BsiSI CCGG 5 cut(s) 358, 384, 480, 2046, 2124
BslFI GGGAC 4 cut(s) 400, 638, 927, 1690
BslI CCNNNNNNNGG 2 cut(s) 384, 1283
BsmAI GTCTC 7 cut(s) 359, 986, 1130, 1376, 1554, 1901, 2051
BsmBI CGTCTC 2 cut(s) 1554, 1901
BsmFI GGGAC 4 cut(s) 400, 638, 927, 1690
BsmI GAATGC 1 cut(s) 1862
BsnI GGCC 3 cut(s) 50, 1804, 1969
Bso31I GGTCTC 2 cut(s) 986, 1376
BsoBI CYCGRG 1 cut(s) 2072
Bsp119I TTCGAA 2 cut(s) 879, 1077
Bsp1286I GDGCHC 3 cut(s) 12, 533, 766
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 7 cut(s) 36, 70, 1127, 1303, 1460, 1645, 1693
Bsp19I CCATGG 2 cut(s) 1989, 2027
BspANI GGCC 3 cut(s) 50, 1804, 1969
BspCNI CTCAG 4 cut(s) 996, 1348, 1544, 2053
BspFNI CGCG 2 cut(s) 94, 1630
BspHI TCATGA 2 cut(s) 457, 748
BspLI GGNNCC 8 cut(s) 388, 443, 583, 887, 933, 1462, 1695, 2122
BspMAI CTGCAG 2 cut(s) 1159, 1510
BspMI ACCTGC 1 cut(s) 657
BspPI GGATC 5 cut(s) 1135, 1455, 1468, 1688, 1701
BspT104I TTCGAA 2 cut(s) 879, 1077
BspT107I GGYRCC 3 cut(s) 441, 581, 2120
BspTNI GGTCTC 2 cut(s) 986, 1376
BsrFI RCCGGY 1 cut(s) 2123
BsrGI TGTACA 1 cut(s) 339
BsrI ACTGG 6 cut(s) 743, 853, 862, 1404, 1671, 2152
BssAI RCCGGY 1 cut(s) 2123
BssECI CCNNGG 3 cut(s) 1204, 1989, 2027
BssMI GATC 7 cut(s) 36, 70, 1127, 1303, 1460, 1645, 1693
BssNI GRCGYC 1 cut(s) 1737
BssT1I CCWWGG 3 cut(s) 1204, 1989, 2027
Bst2UI CCWGG 3 cut(s) 873, 975, 1801
BstACI GRCGYC 1 cut(s) 1737
BstAUI TGTACA 1 cut(s) 339
BstBAI YACGTR 1 cut(s) 2098
BstBI TTCGAA 2 cut(s) 879, 1077
BstC8I GCNNGC 6 cut(s) 261, 793, 869, 1510, 1828, 2162
BstDEI CTNAG 4 cut(s) 983, 1335, 1531, 2061
BstDSI CCRYGG 2 cut(s) 1989, 2027
BstEII GGTNACC 1 cut(s) 2076
BstF5I GGATG 6 cut(s) 24, 488, 558, 1594, 2010, 2023
BstFNI CGCG 2 cut(s) 94, 1630
BstHHI GCGC 2 cut(s) 94, 1514
BstKTI GATC 7 cut(s) 39, 73, 1130, 1306, 1463, 1648, 1696
BstMAI GTCTC 7 cut(s) 359, 986, 1130, 1376, 1554, 1901, 2051
BstMBI GATC 7 cut(s) 36, 70, 1127, 1303, 1460, 1645, 1693
BstMWI GCNNNNNNNGC 7 cut(s) 56, 62, 65, 873, 1827, 1931, 2046
BstNI CCWGG 3 cut(s) 873, 975, 1801
BstNSI RCATGY 3 cut(s) 340, 1442, 2164
BstPI GGTNACC 1 cut(s) 2076
BstSCI CCNGG 4 cut(s) 383, 871, 973, 1799
BstSFI CTRYAG 3 cut(s) 1155, 1506, 1741
BstSLI GKGCMC 2 cut(s) 12, 766
BstUI CGCG 2 cut(s) 94, 1630
BstV1I GCAGC 7 cut(s) 68, 71, 74, 77, 401, 651, 819
BstV2I GAAGAC 1 cut(s) 1054
BstX2I RGATCY 3 cut(s) 1127, 1460, 1693
BstYI RGATCY 3 cut(s) 1127, 1460, 1693
BsuI GTATCC 1 cut(s) 1679
BsuRI GGCC 3 cut(s) 50, 1804, 1969
BtgI CCRYGG 2 cut(s) 1989, 2027
BtrI CACGTC 1 cut(s) 1564
BtsCI GGATG 6 cut(s) 24, 488, 558, 1594, 2010, 2023
BtsIMutI CAGTG 4 cut(s) 112, 517, 869, 1664
BveI ACCTGC 1 cut(s) 657
Cac8I GCNNGC 6 cut(s) 261, 793, 869, 1510, 1828, 2162
CaiI CAGNNNCTG 2 cut(s) 68, 1505
CciI TCATGA 2 cut(s) 457, 748
CfoI GCGC 2 cut(s) 94, 1514
Cfr10I RCCGGY 1 cut(s) 2123
Cfr13I GGNCC 2 cut(s) 387, 822
CpoI CGGWCCG 1 cut(s) 822
CseI GACGC 1 cut(s) 1687
Csp6I GTAC 6 cut(s) 340, 442, 472, 1072, 1411, 2065
CspI CGGWCCG 1 cut(s) 822
CviQI GTAC 6 cut(s) 340, 442, 472, 1072, 1411, 2065
DdeI CTNAG 4 cut(s) 983, 1335, 1531, 2061
DpnI GATC 7 cut(s) 38, 72, 1129, 1305, 1462, 1647, 1695
DpnII GATC 7 cut(s) 36, 70, 1127, 1303, 1460, 1645, 1693
EaeI YGGCCR 1 cut(s) 48
Eco130I CCWWGG 3 cut(s) 1204, 1989, 2027
Eco147I AGGCCT 2 cut(s) 1804, 1969
Eco31I GGTCTC 2 cut(s) 986, 1376
Eco47I GGWCC 2 cut(s) 387, 822
Eco57I CTGAAG 2 cut(s) 285, 1151
Eco88I CYCGRG 1 cut(s) 2072
Eco91I GGTNACC 1 cut(s) 2076
EcoO65I GGTNACC 1 cut(s) 2076
EcoRII CCWGG 3 cut(s) 871, 973, 1799
EcoT14I CCWWGG 3 cut(s) 1204, 1989, 2027
EcoT22I ATGCAT 1 cut(s) 488
ErhI CCWWGG 3 cut(s) 1204, 1989, 2027
Esp3I CGTCTC 2 cut(s) 1554, 1901
FalI AAGNNNNNCTT 2 cut(s) 1665, 1697
FaqI GGGAC 4 cut(s) 400, 638, 927, 1690
FbaI TGATCA 2 cut(s) 36, 1303
Fnu4HI GCNGC 9 cut(s) 57, 60, 63, 66, 415, 640, 808, 1090, 2091
FokI GGATG 6 cut(s) 31, 495, 545, 1601, 2010, 2017
Fsp4HI GCNGC 9 cut(s) 57, 60, 63, 66, 415, 640, 808, 1090, 2091
FspBI CTAG 9 cut(s) 23, 351, 564, 767, 1052, 1116, 1643, 1760, 1823
GlaI GCGC 2 cut(s) 93, 1513
GluI GCNGC 9 cut(s) 57, 60, 63, 66, 415, 640, 808, 1090, 2091
GsuI CTGGAG 1 cut(s) 996
HaeIII GGCC 3 cut(s) 50, 1804, 1969
HapII CCGG 5 cut(s) 358, 384, 480, 2046, 2124
HgaI GACGC 1 cut(s) 1687
HhaI GCGC 2 cut(s) 94, 1514
Hin1I GRCGYC 1 cut(s) 1737
Hin6I GCGC 2 cut(s) 92, 1512
HinP1I GCGC 2 cut(s) 92, 1512
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HindIII AAGCTT 1 cut(s) 1828
HinfI GANTC 5 cut(s) 75, 570, 1059, 1864, 1927
HpaI GTTAAC 1 cut(s) 520
HpaII CCGG 5 cut(s) 358, 384, 480, 2046, 2124
HphI GGTGA 7 cut(s) 239, 281, 367, 469, 963, 1220, 2070
Hpy166II GTNNAC 6 cut(s) 10, 520, 672, 764, 1023, 1231
Hpy188I TCNGA 5 cut(s) 822, 1170, 1338, 1501, 1615
Hpy188III TCNNGA 8 cut(s) 458, 506, 749, 899, 1381, 1643, 1697, 1874
Hpy8I GTNNAC 6 cut(s) 10, 520, 672, 764, 1023, 1231
Hpy99I CGWCG 1 cut(s) 830
HpyAV CCTTC 9 cut(s) 148, 260, 382, 656, 658, 746, 875, 1205, 1994
HpyCH4IV ACGT 4 cut(s) 1563, 1737, 1983, 2097
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 62, 65, 873, 1827, 1931, 2046
HpyF3I CTNAG 4 cut(s) 983, 1335, 1531, 2061
HpySE526I ACGT 4 cut(s) 1563, 1737, 1983, 2097
Hsp92I GRCGYC 1 cut(s) 1737
HspAI GCGC 2 cut(s) 92, 1512
KpnI GGTACC 1 cut(s) 445
Ksp22I TGATCA 2 cut(s) 36, 1303
KspAI GTTAAC 1 cut(s) 520
Kzo9I GATC 7 cut(s) 36, 70, 1127, 1303, 1460, 1645, 1693
LmnI GCTCC 3 cut(s) 937, 1622, 1765
Lsp1109I GCAGC 7 cut(s) 68, 71, 74, 77, 401, 651, 819
LweI GCATC 6 cut(s) 9, 473, 1505, 1808, 1867, 2032
MaeI CTAG 9 cut(s) 23, 351, 564, 767, 1052, 1116, 1643, 1760, 1823
MaeII ACGT 4 cut(s) 1563, 1737, 1983, 2097
MaeIII GTNAC 7 cut(s) 191, 244, 449, 497, 1733, 2076, 2137
MalI GATC 7 cut(s) 38, 72, 1129, 1305, 1462, 1647, 1695
MboI GATC 7 cut(s) 36, 70, 1127, 1303, 1460, 1645, 1693
MboII GAAGA 9 cut(s) 476, 671, 710, 887, 988, 1059, 1091, 1501, 1833
MfeI CAATTG 1 cut(s) 421
MflI RGATCY 3 cut(s) 1127, 1460, 1693
MhlI GDGCHC 3 cut(s) 12, 533, 766
MlsI TGGCCA 1 cut(s) 50
MluNI TGGCCA 1 cut(s) 50
MlyI GAGTC 2 cut(s) 564, 1053
MmeI TCCRAC 2 cut(s) 255, 1316
Mox20I TGGCCA 1 cut(s) 50
Mph1103I ATGCAT 1 cut(s) 488
MroXI GAANNNNTTC 2 cut(s) 176, 1704
MscI TGGCCA 1 cut(s) 50
MseI TTAA 6 cut(s) 519, 735, 1013, 1094, 1314, 2177
MslI CAYNNNNRTG 5 cut(s) 44, 617, 1004, 1194, 1350
Msp20I TGGCCA 1 cut(s) 50
MspA1I CMGCKG 3 cut(s) 68, 810, 2090
MspI CCGG 5 cut(s) 358, 384, 480, 2046, 2124
MspR9I CCNGG 4 cut(s) 385, 873, 975, 1801
MunI CAATTG 1 cut(s) 421
Mva1269I GAATGC 1 cut(s) 1862
MvaI CCWGG 3 cut(s) 873, 975, 1801
MvnI CGCG 2 cut(s) 94, 1630
MwoI GCNNNNNNNGC 7 cut(s) 56, 62, 65, 873, 1827, 1931, 2046
NciI CCSGG 1 cut(s) 385
NcoI CCATGG 2 cut(s) 1989, 2027
NdeII GATC 7 cut(s) 36, 70, 1127, 1303, 1460, 1645, 1693
NlaIV GGNNCC 8 cut(s) 388, 443, 583, 887, 933, 1462, 1695, 2122
NmuCI GTSAC 2 cut(s) 1733, 2076
NsiI ATGCAT 1 cut(s) 488
NspI RCATGY 3 cut(s) 340, 1442, 2164
NspV TTCGAA 2 cut(s) 879, 1077
OliI CACNNNNGTG 1 cut(s) 617
PaeI GCATGC 1 cut(s) 2164
PagI TCATGA 2 cut(s) 457, 748
PceI AGGCCT 2 cut(s) 1804, 1969
PciI ACATGT 1 cut(s) 336
PcsI WCGNNNNNNNCGW 1 cut(s) 91
PctI GAATGC 1 cut(s) 1862
PdmI GAANNNNTTC 2 cut(s) 176, 1704
PfeI GAWTC 3 cut(s) 75, 1864, 1927
PflMI CCANNNNNTGG 1 cut(s) 1283
PfoI TCCNGGA 1 cut(s) 383
PkrI GCNGC 9 cut(s) 58, 61, 64, 67, 416, 641, 809, 1091, 2092
PleI GAGTC 2 cut(s) 564, 1053
PpsI GAGTC 2 cut(s) 564, 1053
Ppu21I YACGTR 1 cut(s) 2098
PscI ACATGT 1 cut(s) 336
Psp6I CCWGG 3 cut(s) 871, 973, 1799
PspEI GGTNACC 1 cut(s) 2076
PspGI CCWGG 3 cut(s) 871, 973, 1799
PspN4I GGNNCC 8 cut(s) 388, 443, 583, 887, 933, 1462, 1695, 2122
PspPI GGNCC 2 cut(s) 387, 822
PstI CTGCAG 2 cut(s) 1159, 1510
PstNI CAGNNNCTG 2 cut(s) 68, 1505
PsuI RGATCY 3 cut(s) 1127, 1460, 1693
PvuII CAGCTG 2 cut(s) 68, 810
RsaI GTAC 6 cut(s) 341, 443, 473, 1073, 1412, 2066
RsaNI GTAC 6 cut(s) 340, 442, 472, 1072, 1411, 2065
RseI CAYNNNNRTG 5 cut(s) 44, 617, 1004, 1194, 1350
Rsr2I CGGWCCG 1 cut(s) 822
RsrII CGGWCCG 1 cut(s) 822
SaqAI TTAA 6 cut(s) 519, 735, 1013, 1094, 1314, 2177
SatI GCNGC 9 cut(s) 57, 60, 63, 66, 415, 640, 808, 1090, 2091
Sau3AI GATC 7 cut(s) 36, 70, 1127, 1303, 1460, 1645, 1693
Sau96I GGNCC 2 cut(s) 387, 822
ScaI AGTACT 2 cut(s) 1073, 2066
SchI GAGTC 2 cut(s) 564, 1053
ScrFI CCNGG 4 cut(s) 385, 873, 975, 1801
SduI GDGCHC 3 cut(s) 12, 533, 766
SfaNI GCATC 6 cut(s) 9, 473, 1505, 1808, 1867, 2032
SfcI CTRYAG 3 cut(s) 1155, 1506, 1741
SfuI TTCGAA 2 cut(s) 879, 1077
SinI GGWCC 2 cut(s) 387, 822
SmiMI CAYNNNNRTG 5 cut(s) 44, 617, 1004, 1194, 1350
SpeI ACTAGT 1 cut(s) 1051
SphI GCATGC 1 cut(s) 2164
SseBI AGGCCT 2 cut(s) 1804, 1969
SspMI CTAG 9 cut(s) 23, 351, 564, 767, 1052, 1116, 1643, 1760, 1823
StuI AGGCCT 2 cut(s) 1804, 1969
StyD4I CCNGG 4 cut(s) 383, 871, 973, 1799
StyI CCWWGG 3 cut(s) 1204, 1989, 2027
TaiI ACGT 4 cut(s) 1566, 1740, 1986, 2100
TaqI TCGA 7 cut(s) 73, 828, 879, 1077, 1380, 1434, 2132
TaqII GACCGA 1 cut(s) 1400
TatI WGTACW 4 cut(s) 339, 1071, 1410, 2064
TauI GCSGC 2 cut(s) 1092, 2093
TfiI GAWTC 3 cut(s) 75, 1864, 1927
Tru1I TTAA 6 cut(s) 519, 735, 1013, 1094, 1314, 2177
Tru9I TTAA 6 cut(s) 519, 735, 1013, 1094, 1314, 2177
TscAI CASTG 4 cut(s) 119, 517, 869, 1671
TseFI GTSAC 2 cut(s) 1733, 2076
TseI GCWGC 7 cut(s) 56, 59, 62, 65, 414, 639, 807
Tsp45I GTSAC 2 cut(s) 1733, 2076
TspRI CASTG 4 cut(s) 119, 517, 869, 1671
Van91I CCANNNNNTGG 1 cut(s) 1283
VneI GTGCAC 2 cut(s) 8, 762
VpaK11BI GGWCC 2 cut(s) 387, 822
XapI RAATTY 4 cut(s) 967, 1869, 2083, 2166
XbaI TCTAGA 1 cut(s) 1642
XceI RCATGY 3 cut(s) 340, 1442, 2164
XcmI CCANNNNNNNNNTGG 2 cut(s) 219, 971
XmnI GAANNNNTTC 2 cut(s) 176, 1704
XspI CTAG 9 cut(s) 23, 351, 564, 767, 1052, 1116, 1643, 1760, 1823
ZraI GACGTC 1 cut(s) 1738
ZrmI AGTACT 2 cut(s) 1073, 2066
Zsp2I ATGCAT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.