RchiOBHm_Chr5g0019001

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000066
Physical Location & Seq
Reverse (-)
13458808 .. 13461822
3015 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29916

Sequence Viewer

Length: 2325 bp
ATGGTCTTATACCGGAGAATGGTCATTTCGCAGCTGATCTCATTGGTAGTGGTGGCCATAGCTGCAGTAGCAATAGCCGACCAAGCCCTCCCGCCTCCTCAAGCCCTCCCTGAATGCCTTGACCACTGTGGCAATCTCACAGTCCCCTATCCATTCGGCATAGGCGACGGCTGTTACCTTACAAAAAAATTCAGCCTCACCTGTAACGAATCCAGCCAACCTCCAACACTACTCTGGGCGGATGGTGGTATCACTACTACTAACATATCCGTGGCCGAAGGTGAGTTGCAAATTTTGGCTAACATAGCCCGTGATTGTTACGACCAAACGGGTCATCAAGGCATGTACAATTTCCCATGGCTATGGGTCTCTCCTCCGTTCACCGTCTCTGGTACCAGAAACAAGTTCATTGCCGTTGGCTATGACACCTATGCCGTTTTTAGAGGGTACCGCCATTCCCCTAATGATAAGGCTAGGCACGTAACTGGATGCATGTCCGTAAGTGACATTCTTGACAATACCACTACTGCTGCTAACGAGCCATGCAAAGGCATTGGGTGTTGCCAAACCTCAATCCCTGACGGACTCAAAAATTATACTATCGAGATAAGCAGTTACTCTAACCATATCTTTGTATGGAATTTCAACCCGTGCAGCTACGCTTTCATTGTGGAAGAAGGCAAGTTCACATTTAACCCGAATACAATTTTTCAAGAACTGCAAAACAATGAACTGCTTCCTCTGATTCTTAATTGGGAAATCGCGGACGGGTCATGTGCTGAAGCTCAAAAGAGGCAGGATTATGCTTGCAAGGCTCATAGCAAGTGTGTCAACCGGACCATCGACATCAAGACTAAACCGTCTGGTTATTATTGCCAGTGCTTGCCAGGCTACCAAGGGAACCCATATCTCCCAGATGGTTGTCAAGATATTGATGAGTGCATCGCTTCAACGAACCCCTGCAACAATGGAACGTGCACAAATTCAGATGGAGATTACACATGTACATGTAACAAAGGTTTCAGAAACGAAAACCCAAAGAGTTGCATACAGAACACTCCTATATCAAAGAACAAGTCCCTGAAAGTTTCCTTGGGTGTCTCTCTGAGCTTCTTAGGAGCATTGCTTGTAACATTTTGGATATACTGCGGACTGAAGAGAAGAAAGTTCAAGATACTCAAAGAGAAGTACTTCAAACACAATGGTGGCTACTTGTTACAACAAAAATTGGAGAATTTCGATGGACCTCAAGCAGCCAAAATATTTACTAGAGAAGAACTTAAGAAGGCCACAAACAACTTTCATGACAGTTTAAAAATTGGTGAAGGAGGCTATGGATTTGTTTATAAAGGAACGCTATCAAATGGAAATGAGGTTGCCATAAAGGTGTCAAAAAGTAGTGCCCCAATGACCCAAAGTAATCAGTTCATTAATGAGGTGATTGTTCTTTCTCAGATCAACCACAGAAATGTGGTGAAGCTTTTAGGTTGTTGTTTGGAGACCCAAACGCCTTTATTAGTGTATGAGTTCGTCAGCAACGGCACACTTTATGAGCATATTCATAAAAAGAATGGCAAGGGACCGCTTTCCTTTGGGCTACGAATGAAGATAGCAACAGAAACAGCAGGATCACTAGCATACTTGCACTACTCTACTTCGATGCAAATTGTGCACCGAGATGTGAAGGCAGCAAACATTCTACTGGATGAGAATTTCACAGCGAAAGTGTCAGACTTTGGAGCTTCAAAATTGGTTCCCGAAGATCAAAATCAACTGTCTACTTTAGTGCAAGGGACGATAGGGTACTTGGACCCTGAATATCTTCAGTCAAACACACTGACAGAGAAAAGTGACGTATATAGCTTCGGCGTTGTCCTTGTGGAGCTAATAACAAGTCAAGTGGCAATTTCTTATCAGAAGCCTGAGGCGGAGAGAAGCTTAGCAAAATTTTTTGTTACTTCTGTAGAAGAGAATCGCCTAGATCAGATTCTCGATCATGAAATAATCAAAGAGGGGAGCTTCGAAACAACTGAACAAGTAGCCCATCTGGCGAAAAGTTGTTTAAGTTTGAATGGGAGCGATAGGCCTACCATGAAAGAAGTAGCAATGAAGCTAGAGGGAGTATTGCAAGTTATGGCAAAGCATCCATGGGGAAAGGCTGATGCCTCACCAAAAGAGACTGACTACTTGCAGCTTGCGTCACATTCAAAAGCCTACGTTGTTGATGTTAGAGGTGATGAAGGTGAAATTATAACCAGTATTGACTACGACCGGAGCATCCAGATTCAACCCCTGATGATGAAGCCTTATGATGGAGGCCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK cEGF

Protein Analysis

774

Amino Acids

85.82

Weight (kDa)

6.22

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 39 - 98 2.5e-09 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 310 - 349 1.9e-07 Calcium-binding EGF domain
Pkinase PF00069 435 - 702 1.5e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 439 - 705 1.7e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1347, 2252
AasI GACNNNNNNGTC 1 cut(s) 859
Acc65I GGTACC 2 cut(s) 392, 447
AccB1I GGYRCC 2 cut(s) 392, 447
AccI GTMKAC 1 cut(s) 1778
AccII CGCG 1 cut(s) 764
AciI CCGC 7 cut(s) 92, 239, 451, 764, 1149, 1583, 1928
AclWI GGATC 1 cut(s) 1636
AcoI YGGCCR 2 cut(s) 54, 273
AcsI RAATTY 7 cut(s) 188, 291, 640, 982, 1234, 1711, 1946
AcuI CTGAAG 3 cut(s) 801, 1175, 1808
AfaI GTAC 6 cut(s) 347, 394, 449, 1006, 1190, 1805
AfiI CCNNNNNNNGG 3 cut(s) 19, 548, 2312
AflII CTTAAG 1 cut(s) 1280
AflIII ACRYGT 2 cut(s) 1001, 1007
AgsI TTSAA 9 cut(s) 646, 713, 951, 1171, 1195, 1746, 2071, 2208, 2288
AjnI CCWGG 1 cut(s) 886
AjuI GAANNNNNNNTTGG 2 cut(s) 1076, 1108
AleI CACNNNNGTG 1 cut(s) 1203
Alw21I GWGCWC 2 cut(s) 980, 1674
Alw26I GTCTC 5 cut(s) 373, 391, 1105, 1493, 2171
Alw44I GTGCAC 2 cut(s) 976, 1670
AlwI GGATC 1 cut(s) 1636
AoxI GGCC 5 cut(s) 54, 273, 1287, 2084, 2317
ApaLI GTGCAC 2 cut(s) 976, 1670
ApeKI GCWGC 7 cut(s) 31, 62, 530, 654, 1253, 1688, 2191
ApoI RAATTY 7 cut(s) 188, 291, 640, 982, 1234, 1711, 1946
ArsI GACNNNNNNTTYG 2 cut(s) 1061, 1093
AseI ATTAAT 1 cut(s) 1431
Asp700I GAANNNNTTC 3 cut(s) 735, 1190, 1821
Asp718I GGTACC 2 cut(s) 392, 447
AspS9I GGNCC 4 cut(s) 837, 1244, 1580, 1810
AsuHPI GGTGA 9 cut(s) 190, 293, 373, 1334, 1450, 1486, 2160, 2246, 2255
AsuII TTCGAA 1 cut(s) 2022
AvaII GGWCC 4 cut(s) 837, 1244, 1580, 1810
AxyI CCTNAGG 1 cut(s) 1923
BaeGI GKGCMC 3 cut(s) 980, 1405, 1674
BalI TGGCCA 1 cut(s) 56
BanI GGYRCC 2 cut(s) 392, 447
Bbv12I GWGCWC 2 cut(s) 980, 1674
BbvI GCAGC 7 cut(s) 43, 49, 517, 666, 1265, 1700, 2203
BccI CCATC 7 cut(s) 236, 848, 911, 983, 1235, 2052, 2306
BceAI ACGGC 4 cut(s) 184, 398, 419, 1555
BciT130I CCWGG 1 cut(s) 888
BcoDI GTCTC 5 cut(s) 373, 391, 1105, 1493, 2171
BfaI CTAG 5 cut(s) 474, 1269, 1634, 1979, 2114
BfmI CTRYAG 2 cut(s) 63, 1962
BfrI CTTAAG 1 cut(s) 1280
BglI GCCNNNNNGGC 1 cut(s) 2048
BisI GCNGC 7 cut(s) 32, 63, 531, 655, 1254, 1689, 2192
BlpI GCTNAGC 1 cut(s) 1939
BlsI GCNGC 7 cut(s) 33, 64, 532, 656, 1255, 1690, 2193
BmcAI AGTACT 1 cut(s) 1190
Bme1390I CCNGG 1 cut(s) 888
Bme18I GGWCC 4 cut(s) 837, 1244, 1580, 1810
BmgT120I GGNCC 4 cut(s) 837, 1244, 1580, 1810
BmiI GGNNCC 6 cut(s) 394, 449, 902, 1581, 1755, 1812
BmrFI CCNGG 1 cut(s) 888
BmsI GCATC 6 cut(s) 479, 951, 1650, 2152, 2152, 2286
Bpu1102I GCTNAGC 1 cut(s) 1939
Bpu14I TTCGAA 1 cut(s) 2022
BpuEI CTTGAG 2 cut(s) 84, 1233
BsaAI YACGTR 1 cut(s) 481
BsaBI GATNNNNATC 1 cut(s) 1767
BsaI GGTCTC 2 cut(s) 373, 1493
BsaJI CCNNGG 5 cut(s) 270, 356, 895, 1092, 2147
BsaWI WCCGGW 3 cut(s) 12, 834, 2271
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 548, 2312
Bse1I ACTGG 4 cut(s) 490, 877, 1707, 2256
Bse21I CCTNAGG 1 cut(s) 1923
Bse3DI GCAATG 3 cut(s) 408, 1121, 2112
Bse8I GATNNNNATC 1 cut(s) 1767
BseBI CCWGG 1 cut(s) 888
BseDI CCNNGG 5 cut(s) 270, 356, 895, 1092, 2147
BseGI GGATG 5 cut(s) 247, 494, 1711, 2143, 2277
BseJI GATNNNNATC 1 cut(s) 1767
BseLI CCNNNNNNNGG 3 cut(s) 19, 548, 2312
BseMI GCAATG 3 cut(s) 408, 1121, 2112
BseMII CTCAG 3 cut(s) 1097, 1466, 1914
BseNI ACTGG 4 cut(s) 490, 877, 1707, 2256
BseRI GAGGAG 2 cut(s) 87, 363
BseSI GKGCMC 3 cut(s) 980, 1405, 1674
BseXI GCAGC 7 cut(s) 43, 49, 517, 666, 1265, 1700, 2203
BsgI GTGCAG 1 cut(s) 673
Bsh1236I CGCG 1 cut(s) 764
Bsh1285I CGRYCG 1 cut(s) 2272
BshFI GGCC 5 cut(s) 56, 275, 1289, 2086, 2319
BshNI GGYRCC 2 cut(s) 392, 447
BsiEI CGRYCG 1 cut(s) 2272
BsiHKAI GWGCWC 2 cut(s) 980, 1674
BsiSI CCGG 3 cut(s) 13, 835, 2272
BslFI GGGAC 4 cut(s) 128, 1063, 1593, 1807
BslI CCNNNNNNNGG 3 cut(s) 19, 548, 2312
BsmAI GTCTC 5 cut(s) 373, 391, 1105, 1493, 2171
BsmBI CGTCTC 1 cut(s) 391
BsmFI GGGAC 4 cut(s) 128, 1063, 1593, 1807
BsmI GAATGC 1 cut(s) 119
BsnI GGCC 5 cut(s) 56, 275, 1289, 2086, 2319
Bso31I GGTCTC 2 cut(s) 373, 1493
Bsp119I TTCGAA 1 cut(s) 2022
Bsp1286I GDGCHC 3 cut(s) 980, 1405, 1674
Bsp1407I TGTACA 2 cut(s) 345, 1004
Bsp143I GATC 6 cut(s) 36, 1455, 1628, 1762, 1981, 1993
Bsp1720I GCTNAGC 1 cut(s) 1939
Bsp19I CCATGG 2 cut(s) 356, 2147
BspACI CCGC 7 cut(s) 92, 239, 451, 764, 1149, 1583, 1928
BspANI GGCC 5 cut(s) 56, 275, 1289, 2086, 2319
BspCNI CTCAG 3 cut(s) 1098, 1465, 1915
BspFNI CGCG 1 cut(s) 764
BspHI TCATGA 2 cut(s) 1303, 1996
BspLI GGNNCC 6 cut(s) 394, 449, 902, 1581, 1755, 1812
BspMAI CTGCAG 1 cut(s) 67
BspPI GGATC 1 cut(s) 1636
BspT104I TTCGAA 1 cut(s) 2022
BspT107I GGYRCC 2 cut(s) 392, 447
BspTI CTTAAG 1 cut(s) 1280
BspTNI GGTCTC 2 cut(s) 373, 1493
BsrDI GCAATG 3 cut(s) 408, 1121, 2112
BsrGI TGTACA 2 cut(s) 345, 1004
BsrI ACTGG 4 cut(s) 490, 877, 1707, 2256
BssECI CCNNGG 5 cut(s) 270, 356, 895, 1092, 2147
BssMI GATC 6 cut(s) 36, 1455, 1628, 1762, 1981, 1993
BssT1I CCWWGG 4 cut(s) 356, 895, 1092, 2147
Bst2UI CCWGG 1 cut(s) 888
Bst4CI ACNGT 6 cut(s) 128, 142, 385, 861, 1310, 1776
Bst6I CTCTTC 2 cut(s) 1151, 1962
BstAFI CTTAAG 1 cut(s) 1280
BstAPI GCANNNNNTGC 1 cut(s) 1669
BstAUI TGTACA 2 cut(s) 345, 1004
BstBAI YACGTR 1 cut(s) 481
BstBI TTCGAA 1 cut(s) 2022
BstC8I GCNNGC 3 cut(s) 808, 884, 2196
BstDEI CTNAG 5 cut(s) 1106, 1114, 1452, 1923, 1939
BstDSI CCRYGG 3 cut(s) 270, 356, 2147
BstF5I GGATG 5 cut(s) 247, 494, 1711, 2143, 2277
BstFNI CGCG 1 cut(s) 764
BstKTI GATC 6 cut(s) 39, 1458, 1631, 1765, 1984, 1996
BstMAI GTCTC 5 cut(s) 373, 391, 1105, 1493, 2171
BstMBI GATC 6 cut(s) 36, 1455, 1628, 1762, 1981, 1993
BstMCI CGRYCG 1 cut(s) 2272
BstMWI GCNNNNNNNGC 8 cut(s) 62, 68, 83, 305, 812, 888, 1669, 2048
BstNI CCWGG 1 cut(s) 888
BstNSI RCATGY 4 cut(s) 346, 496, 1005, 1011
BstSCI CCNGG 1 cut(s) 886
BstSFI CTRYAG 2 cut(s) 63, 1962
BstSLI GKGCMC 3 cut(s) 980, 1405, 1674
BstUI CGCG 1 cut(s) 764
BstV1I GCAGC 7 cut(s) 43, 49, 517, 666, 1265, 1700, 2203
BstXI CCANNNNNNTGG 1 cut(s) 363
Bsu36I CCTNAGG 1 cut(s) 1923
BsuRI GGCC 5 cut(s) 56, 275, 1289, 2086, 2319
BtgI CCRYGG 3 cut(s) 270, 356, 2147
BtgZI GCGATG 1 cut(s) 928
BtsCI GGATG 5 cut(s) 247, 494, 1711, 2143, 2277
BtsIMutI CAGTG 3 cut(s) 124, 884, 1835
Cac8I GCNNGC 3 cut(s) 808, 884, 2196
CciI TCATGA 2 cut(s) 1303, 1996
Cfr13I GGNCC 4 cut(s) 837, 1244, 1580, 1810
CseI GACGC 1 cut(s) 2187
Csp6I GTAC 6 cut(s) 346, 393, 448, 1005, 1189, 1804
CspCI CAANNNNNGTGG 2 cut(s) 1881, 1916
CviQI GTAC 6 cut(s) 346, 393, 448, 1005, 1189, 1804
DdeI CTNAG 5 cut(s) 1106, 1114, 1452, 1923, 1939
DpnI GATC 6 cut(s) 38, 1457, 1630, 1764, 1983, 1995
DpnII GATC 6 cut(s) 36, 1455, 1628, 1762, 1981, 1993
DraI TTTAAA 1 cut(s) 1314
DrdI GACNNNNNNGTC 1 cut(s) 859
DseDI GACNNNNNNGTC 1 cut(s) 859
EaeI YGGCCR 2 cut(s) 54, 273
Eam1104I CTCTTC 2 cut(s) 1151, 1962
EarI CTCTTC 2 cut(s) 1151, 1962
EciI GGCGGA 2 cut(s) 254, 1943
Eco130I CCWWGG 4 cut(s) 356, 895, 1092, 2147
Eco147I AGGCCT 1 cut(s) 2086
Eco31I GGTCTC 2 cut(s) 373, 1493
Eco47I GGWCC 4 cut(s) 837, 1244, 1580, 1810
Eco57I CTGAAG 3 cut(s) 801, 1175, 1808
Eco81I CCTNAGG 1 cut(s) 1923
EcoRII CCWGG 1 cut(s) 886
EcoT14I CCWWGG 4 cut(s) 356, 895, 1092, 2147
EcoT22I ATGCAT 1 cut(s) 494
ErhI CCWWGG 4 cut(s) 356, 895, 1092, 2147
Esp3I CGTCTC 1 cut(s) 391
FaqI GGGAC 4 cut(s) 128, 1063, 1593, 1807
FauI CCCGC 1 cut(s) 99
FblI GTMKAC 1 cut(s) 1778
Fnu4HI GCNGC 7 cut(s) 32, 63, 531, 655, 1254, 1689, 2192
FokI GGATG 5 cut(s) 254, 501, 1718, 2130, 2264
Fsp4HI GCNGC 7 cut(s) 32, 63, 531, 655, 1254, 1689, 2192
FspBI CTAG 5 cut(s) 474, 1269, 1634, 1979, 2114
GluI GCNGC 7 cut(s) 32, 63, 531, 655, 1254, 1689, 2192
HaeIII GGCC 5 cut(s) 56, 275, 1289, 2086, 2319
HapII CCGG 3 cut(s) 13, 835, 2272
HgaI GACGC 1 cut(s) 2187
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HindIII AAGCTT 2 cut(s) 1478, 1936
HinfI GANTC 6 cut(s) 209, 585, 745, 1972, 1987, 2284
HpaII CCGG 3 cut(s) 13, 835, 2272
HphI GGTGA 9 cut(s) 190, 293, 373, 1334, 1450, 1486, 2160, 2246, 2255
Hpy166II GTNNAC 6 cut(s) 381, 687, 832, 978, 1672, 1779
Hpy188I TCNGA 8 cut(s) 744, 988, 1025, 1107, 1455, 1732, 1917, 1986
Hpy8I GTNNAC 6 cut(s) 381, 687, 832, 978, 1672, 1779
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 6 cut(s) 272, 671, 1279, 1319, 1678, 2234
HpyCH4III ACNGT 6 cut(s) 128, 142, 385, 861, 1310, 1776
HpyCH4IV ACGT 4 cut(s) 480, 974, 1854, 2217
HpyF10VI GCNNNNNNNGC 8 cut(s) 62, 68, 83, 305, 812, 888, 1669, 2048
HpyF3I CTNAG 5 cut(s) 1106, 1114, 1452, 1923, 1939
HpySE526I ACGT 4 cut(s) 480, 974, 1854, 2217
KpnI GGTACC 2 cut(s) 396, 451
Kzo9I GATC 6 cut(s) 36, 1455, 1628, 1762, 1981, 1993
LmnI GCTCC 6 cut(s) 1118, 1739, 1882, 2016, 2076, 2274
Lsp1109I GCAGC 7 cut(s) 43, 49, 517, 666, 1265, 1700, 2203
LweI GCATC 6 cut(s) 479, 951, 1650, 2152, 2152, 2286
MaeI CTAG 5 cut(s) 474, 1269, 1634, 1979, 2114
MaeII ACGT 4 cut(s) 480, 974, 1854, 2217
MalI GATC 6 cut(s) 38, 1457, 1630, 1764, 1983, 1995
MboI GATC 6 cut(s) 36, 1455, 1628, 1762, 1981, 1993
MboII GAAGA 8 cut(s) 686, 1168, 1173, 1286, 1618, 1772, 1814, 1979
MhlI GDGCHC 3 cut(s) 980, 1405, 1674
MlsI TGGCCA 1 cut(s) 56
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 1 cut(s) 579
MmeI TCCRAC 1 cut(s) 248
Mox20I TGGCCA 1 cut(s) 56
Mph1103I ATGCAT 1 cut(s) 494
MroXI GAANNNNTTC 3 cut(s) 735, 1190, 1821
MscI TGGCCA 1 cut(s) 56
MseI TTAA 6 cut(s) 693, 750, 1281, 1313, 1431, 2063
MslI CAYNNNNRTG 7 cut(s) 269, 361, 1006, 1203, 1385, 1467, 1677
Msp20I TGGCCA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 34
MspCI CTTAAG 1 cut(s) 1280
MspI CCGG 3 cut(s) 13, 835, 2272
MspR9I CCNGG 1 cut(s) 888
Mva1269I GAATGC 1 cut(s) 119
MvaI CCWGG 1 cut(s) 888
MvnI CGCG 1 cut(s) 764
MwoI GCNNNNNNNGC 8 cut(s) 62, 68, 83, 305, 812, 888, 1669, 2048
NcoI CCATGG 2 cut(s) 356, 2147
NdeII GATC 6 cut(s) 36, 1455, 1628, 1762, 1981, 1993
NlaIV GGNNCC 6 cut(s) 394, 449, 902, 1581, 1755, 1812
NmuCI GTSAC 3 cut(s) 503, 1850, 2199
NsiI ATGCAT 1 cut(s) 494
NspI RCATGY 4 cut(s) 346, 496, 1005, 1011
NspV TTCGAA 1 cut(s) 2022
OliI CACNNNNGTG 1 cut(s) 1203
PagI TCATGA 2 cut(s) 1303, 1996
PceI AGGCCT 1 cut(s) 2086
PciI ACATGT 2 cut(s) 1001, 1007
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PctI GAATGC 1 cut(s) 119
PdmI GAANNNNTTC 3 cut(s) 735, 1190, 1821
PfeI GAWTC 5 cut(s) 209, 745, 1972, 1987, 2284
PkrI GCNGC 7 cut(s) 33, 64, 532, 656, 1255, 1690, 2193
PleI GAGTC 1 cut(s) 579
PpsI GAGTC 1 cut(s) 579
Ppu21I YACGTR 1 cut(s) 481
PscI ACATGT 2 cut(s) 1001, 1007
PshBI ATTAAT 1 cut(s) 1431
PsiI TTATAA 2 cut(s) 1347, 2252
Psp6I CCWGG 1 cut(s) 886
PspGI CCWGG 1 cut(s) 886
PspN4I GGNNCC 6 cut(s) 394, 449, 902, 1581, 1755, 1812
PspPI GGNCC 4 cut(s) 837, 1244, 1580, 1810
PstI CTGCAG 1 cut(s) 67
PvuII CAGCTG 1 cut(s) 34
RsaI GTAC 6 cut(s) 347, 394, 449, 1006, 1190, 1805
RsaNI GTAC 6 cut(s) 346, 393, 448, 1005, 1189, 1804
RseI CAYNNNNRTG 7 cut(s) 269, 361, 1006, 1203, 1385, 1467, 1677
SaqAI TTAA 6 cut(s) 693, 750, 1281, 1313, 1431, 2063
SatI GCNGC 7 cut(s) 32, 63, 531, 655, 1254, 1689, 2192
Sau3AI GATC 6 cut(s) 36, 1455, 1628, 1762, 1981, 1993
Sau96I GGNCC 4 cut(s) 837, 1244, 1580, 1810
ScaI AGTACT 1 cut(s) 1190
SchI GAGTC 1 cut(s) 579
ScrFI CCNGG 1 cut(s) 888
SduI GDGCHC 3 cut(s) 980, 1405, 1674
SfaNI GCATC 6 cut(s) 479, 951, 1650, 2152, 2152, 2286
SfcI CTRYAG 2 cut(s) 63, 1962
SfuI TTCGAA 1 cut(s) 2022
SinI GGWCC 4 cut(s) 837, 1244, 1580, 1810
SmiMI CAYNNNNRTG 7 cut(s) 269, 361, 1006, 1203, 1385, 1467, 1677
SmlI CTYRAG 3 cut(s) 99, 1248, 1280
SmoI CTYRAG 3 cut(s) 99, 1248, 1280
SseBI AGGCCT 1 cut(s) 2086
SsiI CCGC 7 cut(s) 92, 239, 451, 764, 1149, 1583, 1928
SspI AATATT 1 cut(s) 1263
SspMI CTAG 5 cut(s) 474, 1269, 1634, 1979, 2114
StuI AGGCCT 1 cut(s) 2086
StyD4I CCNGG 1 cut(s) 886
StyI CCWWGG 4 cut(s) 356, 895, 1092, 2147
TaaI ACNGT 6 cut(s) 128, 142, 385, 861, 1310, 1776
TaiI ACGT 4 cut(s) 483, 977, 1857, 2220
TaqI TCGA 6 cut(s) 603, 843, 1239, 1658, 1992, 2022
TatI WGTACW 3 cut(s) 345, 1004, 1188
TfiI GAWTC 5 cut(s) 209, 745, 1972, 1987, 2284
Tru1I TTAA 6 cut(s) 693, 750, 1281, 1313, 1431, 2063
Tru9I TTAA 6 cut(s) 693, 750, 1281, 1313, 1431, 2063
TscAI CASTG 3 cut(s) 131, 884, 1842
TseFI GTSAC 3 cut(s) 503, 1850, 2199
TseI GCWGC 7 cut(s) 31, 62, 530, 654, 1253, 1688, 2191
Tsp45I GTSAC 3 cut(s) 503, 1850, 2199
TspGWI ACGGA 4 cut(s) 259, 366, 487, 597
TspRI CASTG 3 cut(s) 131, 884, 1842
Vha464I CTTAAG 1 cut(s) 1280
VneI GTGCAC 2 cut(s) 976, 1670
VpaK11BI GGWCC 4 cut(s) 837, 1244, 1580, 1810
VspI ATTAAT 1 cut(s) 1431
XapI RAATTY 7 cut(s) 188, 291, 640, 982, 1234, 1711, 1946
XceI RCATGY 4 cut(s) 346, 496, 1005, 1011
XcmI CCANNNNNNNNNTGG 1 cut(s) 231
XmiI GTMKAC 1 cut(s) 1778
XmnI GAANNNNTTC 3 cut(s) 735, 1190, 1821
XspI CTAG 5 cut(s) 474, 1269, 1634, 1979, 2114
ZrmI AGTACT 1 cut(s) 1190
Zsp2I ATGCAT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.