Rroxscaffold_4G00326240

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000066
Physical Location & Seq
Forward (+)
58244520 .. 58248844
4325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326240.1

Sequence Viewer

Length: 2265 bp
ATGGCCATACGTGGGATGCTGATTATGCAACCCTCACCGGTCGGAGTATTTATTCTGGTTCTATTATTAGAAGGGTCAGCAGCAGCCGTAACTCAAGCCTTTCCACCTCAATCCAAGCCAGGCTGCACTGATCACTGTGGTAGTCTCACAATTCAATATCCCTTTGGCGTAGAGCCTGGTTGTTACAAGACAGATGCTTTTTCTGTCATTTGTAACACGTCCACTGAACCACCAACTGTAATGTGGGGAACCATCTCTGTTACTGGCATATCTCTGGCTAAGGGTGAGATGCAAATAATGCAGTTCATAGCCAAAGATTGCTATGACCGCAGGGGTGCGACTACTTCCTACTTCAGAACTAGACTCTCGGTGCCATTTCCCTTCACCATATCTCATACAAAGAACAAGTTCGTTGCAGTTGGTTGTGATACTTATGCAATTTTCAGAGGTTACCGTGCAGATGGAGAAAGATACTCATCTGGGTGCATGTCCATATGTGACAATCTCGACAGCGTCGAGAAGTCTTGCTCAGGCATTGGGTGTTGCCACACTGACATCCCTAGTGGACTCAAAAATCGTACTGTCACATTGGATAGCTATTCCAATCATACATCTGTATGGAACTTCAACCCGTGCAGCTACGCCTTTATTGTGGAAGAAGGCAAGTTCACATTTTCCCCGAATACGAGTTTTAAGCAACTGGACAACACTGAAAAGCTTCCGATGATTCTTAATTGGGAAATTGGGGAAGGGACATGTGAGATAGCTAAGAAGAGGGATGATTATGCCTGCAAGGCTAAGAGCAGCAAGTGTATCAATCGGACCATCAGCAATGAGCTGTCTCGTTACTTTTGCCAGTGCCTACCAGATATTGATGAGTGCAAGGCTTCAAACTCCTGCACCAACGGGACATGTGTAAATTCACCTGGAGATTACTCCTGTTTGTGTAATGAAGGGTTCATACACGACGGTGTGAATAACAAAAGTTGCACCCAGAACAATTCCAAAAATCAACCAAAGATCACACTCCTGCTTAAGATATCGTTGGGTTTAAGTGGGGGCTTCTTGGTTTTACTCGTTGGAATTTTGTGGATATGTTGGGGAATAAAGAAAAGACAATTCAGTAAACTCAAAGAAAAGTACTTCAAAGAGAATGGTGGCTACTTGTTGCTACAACAACTTGCTAGTCATGGAAGCTCGTTGGAGACAACAAAAATCTTTACTGCTGAAGAACTAAAGAAAGCAACAAACAATTATCATGAAAGTAGAGTCCTTGGAGAAGGAGGCTATGGAACAGTTTACAAAGGAATACTACCAGATGACAAAGTGGTGGCCATAAAGAAGTCAAAAGGTGGTGCCTCAACCCAAAGTGATCAGTTTGTTAATGAGGTGATTGTTCTTTCTAAAATCAACCACAGAAATGTGGTGAAGCTATTAGGTTGTTGTTTAGAAACCGAAGTACCTTTATTAGTATACGAGTTCATCACCCATGGCACTCTTTATGAGCATATTCATAAAAAAGGTTCACCACTCTCATTTGACTTGAGAATGAAGATAGCAGTTGAAACTGCAGAAGCATTAGCCCATTTGCATTCCTCAATTTCCACACCGATCATACATCGAGATGTGAAAGCAGCAAATATTTTGTTAGATGATAATTATACAGCGAAAGTGTCAGACTTTGGAGCTTCACGATTGGTTCCTTCAGGACAAACAGAAATACAAACGCTAGTGCTCGGGACATTCGGATACCTAGACCCTGAATATCTGCAATCAAACCAACTGACAGAAAAGAGTGATGTTTACAGCTTTGGAGTTGTCCTAGTGGAGCTACTAACAAGCAAAGTGCCTGTTTCTAAAGATAGATGCTTAGCAAGTATCTTTGTTGCTTCCATGGAAGAAGATTGGTTGAATCATATTCTTGATGATGATATAGTGAATGAGCGAAACATTGAGACGGTAAAGGAAGTGGCCAATCTTGCAAAAAGATGCTTGAGGGTAAAAGGAGAGGAAAGGCCTACCATGAAAGAAGTTGCGAAAGAGTTGGAGGAAATGAGTGTCATGGCAAAACATCCTTGGGGATTTAATGCTAATTTCTGCGGAGAAGAGAATGAGTACTTGCTTGGGTCACTTAATTCAGACGCTTATGTTGTGGGTGTTGGTGGAGGTGATTGCAATTCTAGTGGTCTAACTAGTGGTACGACCAATGCATATGACAGCATGCAAAACCAACTGCAGTTGATGCCATACGGTGGTGTACGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

754

Amino Acids

83.02

Weight (kDa)

6.19

Isoelectric Point (pI)

37.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 42 - 100 1.2e-07 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 419 - 680 3.2e-51 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 422 - 682 9.4e-52 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 370, 1355
AccB7I CCANNNNNTGG 1 cut(s) 2252
AccI GTMKAC 1 cut(s) 1473
AciI CCGC 2 cut(s) 328, 2100
AcoI YGGCCR 3 cut(s) 3, 1332, 1971
AcsI RAATTY 2 cut(s) 919, 1083
AcuI CTGAAG 3 cut(s) 337, 1248, 1689
AfaI GTAC 6 cut(s) 580, 1142, 1461, 2117, 2200, 2259
AfiI CCNNNNNNNGG 2 cut(s) 12, 2252
AflII CTTAAG 1 cut(s) 1034
AflIII ACRYGT 3 cut(s) 216, 755, 911
AgeI ACCGGT 1 cut(s) 37
AgsI TTSAA 6 cut(s) 155, 628, 891, 1147, 1565, 1912
AhlI ACTAGT 1 cut(s) 2192
AjiI CACGTC 1 cut(s) 219
AjnI CCWGG 3 cut(s) 118, 175, 925
AleI CACNNNNGTG 1 cut(s) 969
Alw21I GWGCWC 1 cut(s) 1737
Alw26I GTCTC 4 cut(s) 149, 846, 1199, 1949
Ama87I CYCGRG 1 cut(s) 1736
AoxI GGCC 4 cut(s) 3, 1332, 1971, 2015
ApeKI GCWGC 6 cut(s) 80, 83, 123, 636, 804, 1634
ApoI RAATTY 2 cut(s) 919, 1083
AsiGI ACCGGT 1 cut(s) 37
Asp700I GAANNNNTTC 2 cut(s) 407, 717
AspS9I GGNCC 1 cut(s) 822
AsuHPI GGTGA 9 cut(s) 27, 296, 376, 915, 1402, 1438, 1477, 1518, 2180
AvaI CYCGRG 1 cut(s) 1736
AvaII GGWCC 1 cut(s) 822
BaeI ACNNNNGTAYC 2 cut(s) 1443, 1476
BalI TGGCCA 3 cut(s) 5, 1334, 1973
BanI GGYRCC 2 cut(s) 370, 1355
BarI GAAGNNNNNNTAC 2 cut(s) 945, 977
Bbv12I GWGCWC 1 cut(s) 1737
BbvI GCAGC 6 cut(s) 92, 95, 110, 648, 816, 1646
BccI CCATC 3 cut(s) 260, 455, 833
BceAI ACGGC 1 cut(s) 71
BciT130I CCWGG 3 cut(s) 120, 177, 927
BciVI GTATCC 1 cut(s) 1742
BclI TGATCA 2 cut(s) 130, 1372
BcoDI GTCTC 4 cut(s) 149, 846, 1199, 1949
BcuI ACTAGT 1 cut(s) 2192
BfaI CTAG 8 cut(s) 360, 561, 1185, 1730, 1754, 1823, 2181, 2193
BfmI CTRYAG 2 cut(s) 1569, 2234
BfrI CTTAAG 1 cut(s) 1034
BfuI GTATCC 1 cut(s) 1742
BglI GCCNNNNNGGC 1 cut(s) 794
BisI GCNGC 6 cut(s) 81, 84, 124, 637, 805, 1635
BlpI GCTNAGC 1 cut(s) 1870
BlsI GCNGC 6 cut(s) 82, 85, 125, 638, 806, 1636
BmcAI AGTACT 2 cut(s) 1142, 2117
Bme1390I CCNGG 3 cut(s) 120, 177, 927
Bme18I GGWCC 1 cut(s) 822
BmeT110I CYCGRG 1 cut(s) 1736
BmgBI CACGTC 1 cut(s) 219
BmgT120I GGNCC 1 cut(s) 822
BmiI GGNNCC 4 cut(s) 250, 372, 1357, 1701
BmrFI CCNGG 3 cut(s) 120, 177, 927
BmsI GCATC 6 cut(s) 6, 184, 279, 1856, 1979, 2232
BpmI CTGGAG 1 cut(s) 948
Bpu10I CCTNAGC 2 cut(s) 279, 529
Bpu1102I GCTNAGC 1 cut(s) 1870
BpuEI CTTGAG 3 cut(s) 78, 1564, 2014
BsaAI YACGTR 1 cut(s) 11
BsaBI GATNNNNATC 1 cut(s) 475
BsaJI CCNNGG 4 cut(s) 1273, 1489, 1893, 2075
BsaWI WCCGGW 1 cut(s) 37
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 2252
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 3 cut(s) 268, 705, 856
Bse3DI GCAATG 1 cut(s) 838
Bse8I GATNNNNATC 1 cut(s) 475
BseBI CCWGG 3 cut(s) 120, 177, 927
BseDI CCNNGG 4 cut(s) 1273, 1489, 1893, 2075
BseGI GGATG 4 cut(s) 21, 555, 784, 2071
BseJI GATNNNNATC 1 cut(s) 475
BseLI CCNNNNNNNGG 2 cut(s) 12, 2252
BseMI GCAATG 1 cut(s) 838
BseMII CTCAG 1 cut(s) 543
BseNI ACTGG 3 cut(s) 268, 705, 856
BseXI GCAGC 6 cut(s) 92, 95, 110, 648, 816, 1646
BsgI GTGCAG 4 cut(s) 109, 477, 655, 883
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 4 cut(s) 5, 1334, 1973, 2017
BshNI GGYRCC 2 cut(s) 370, 1355
BshTI ACCGGT 1 cut(s) 37
BsiEI CGRYCG 1 cut(s) 42
BsiHKAI GWGCWC 1 cut(s) 1737
BsiHKCI CYCGRG 1 cut(s) 1736
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 3 cut(s) 766, 922, 1753
BslI CCNNNNNNNGG 2 cut(s) 12, 2252
BsmAI GTCTC 4 cut(s) 149, 846, 1199, 1949
BsmBI CGTCTC 1 cut(s) 1949
BsmFI GGGAC 3 cut(s) 766, 922, 1753
BsmI GAATGC 1 cut(s) 1591
BsnI GGCC 4 cut(s) 5, 1334, 1973, 2017
BsoBI CYCGRG 1 cut(s) 1736
Bsp1286I GDGCHC 1 cut(s) 1737
Bsp143I GATC 4 cut(s) 130, 1020, 1372, 1611
Bsp1720I GCTNAGC 1 cut(s) 1870
Bsp19I CCATGG 2 cut(s) 1489, 1893
BspACI CCGC 2 cut(s) 328, 2100
BspANI GGCC 4 cut(s) 5, 1334, 1973, 2017
BspCNI CTCAG 1 cut(s) 542
BspHI TCATGA 1 cut(s) 1258
BspLI GGNNCC 4 cut(s) 250, 372, 1357, 1701
BspMAI CTGCAG 2 cut(s) 1573, 2238
BspT107I GGYRCC 2 cut(s) 370, 1355
BspTI CTTAAG 1 cut(s) 1034
BsrDI GCAATG 1 cut(s) 838
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 3 cut(s) 268, 705, 856
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 4 cut(s) 1273, 1489, 1893, 2075
BssMI GATC 4 cut(s) 130, 1020, 1372, 1611
BssNAI GTATAC 1 cut(s) 1474
BssT1I CCWWGG 4 cut(s) 1273, 1489, 1893, 2075
Bst1107I GTATAC 1 cut(s) 1474
Bst2UI CCWGG 3 cut(s) 120, 177, 927
Bst4CI ACNGT 9 cut(s) 137, 238, 455, 583, 971, 1297, 1960, 2252, 2262
Bst6I CTCTTC 2 cut(s) 767, 2100
BstAFI CTTAAG 1 cut(s) 1034
BstAPI GCANNNNNTGC 2 cut(s) 298, 2242
BstBAI YACGTR 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 790, 2222
BstDEI CTNAG 5 cut(s) 279, 529, 768, 798, 1870
BstDSI CCRYGG 2 cut(s) 1489, 1893
BstEII GGTNACC 1 cut(s) 449
BstF5I GGATG 4 cut(s) 21, 555, 784, 2071
BstKTI GATC 4 cut(s) 133, 1023, 1375, 1614
BstMAI GTCTC 4 cut(s) 149, 846, 1199, 1949
BstMBI GATC 4 cut(s) 130, 1020, 1372, 1611
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 6 cut(s) 25, 298, 327, 794, 1979, 2242
BstNI CCWGG 3 cut(s) 120, 177, 927
BstNSI RCATGY 4 cut(s) 490, 759, 915, 2224
BstPI GGTNACC 1 cut(s) 449
BstSCI CCNGG 3 cut(s) 118, 175, 925
BstSFI CTRYAG 2 cut(s) 1569, 2234
BstV1I GCAGC 6 cut(s) 92, 95, 110, 648, 816, 1646
BstZ17I GTATAC 1 cut(s) 1474
BsuI GTATCC 1 cut(s) 1742
BsuRI GGCC 4 cut(s) 5, 1334, 1973, 2017
BtgI CCRYGG 2 cut(s) 1489, 1893
BtrI CACGTC 1 cut(s) 219
BtsCI GGATG 4 cut(s) 21, 555, 784, 2071
BtsIMutI CAGTG 6 cut(s) 126, 133, 222, 549, 708, 863
Cac8I GCNNGC 2 cut(s) 790, 2222
CciI TCATGA 1 cut(s) 1258
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 1 cut(s) 822
CseI GACGC 2 cut(s) 502, 2150
Csp6I GTAC 6 cut(s) 579, 1141, 1460, 2116, 2199, 2258
CspAI ACCGGT 1 cut(s) 37
CspCI CAANNNNNGTGG 2 cut(s) 2164, 2199
CviQI GTAC 6 cut(s) 579, 1141, 1460, 2116, 2199, 2258
DdeI CTNAG 5 cut(s) 279, 529, 768, 798, 1870
DpnI GATC 4 cut(s) 132, 1022, 1374, 1613
DpnII GATC 4 cut(s) 130, 1020, 1372, 1611
EaeI YGGCCR 3 cut(s) 3, 1332, 1971
Eam1104I CTCTTC 2 cut(s) 767, 2100
EarI CTCTTC 2 cut(s) 767, 2100
Eco130I CCWWGG 4 cut(s) 1273, 1489, 1893, 2075
Eco147I AGGCCT 1 cut(s) 2017
Eco32I GATATC 1 cut(s) 1041
Eco47I GGWCC 1 cut(s) 822
Eco57I CTGAAG 3 cut(s) 337, 1248, 1689
Eco88I CYCGRG 1 cut(s) 1736
Eco91I GGTNACC 1 cut(s) 449
EcoO65I GGTNACC 1 cut(s) 449
EcoRII CCWGG 3 cut(s) 118, 175, 925
EcoRV GATATC 1 cut(s) 1041
EcoT14I CCWWGG 4 cut(s) 1273, 1489, 1893, 2075
EcoT22I ATGCAT 1 cut(s) 2212
ErhI CCWWGG 4 cut(s) 1273, 1489, 1893, 2075
Esp3I CGTCTC 1 cut(s) 1949
FaqI GGGAC 3 cut(s) 766, 922, 1753
FauNDI CATATG 2 cut(s) 494, 2212
FbaI TGATCA 2 cut(s) 130, 1372
FblI GTMKAC 1 cut(s) 1473
Fnu4HI GCNGC 6 cut(s) 81, 84, 124, 637, 805, 1635
FokI GGATG 4 cut(s) 28, 542, 791, 2058
Fsp4HI GCNGC 6 cut(s) 81, 84, 124, 637, 805, 1635
FspBI CTAG 8 cut(s) 360, 561, 1185, 1730, 1754, 1823, 2181, 2193
GluI GCNGC 6 cut(s) 81, 84, 124, 637, 805, 1635
GsuI CTGGAG 1 cut(s) 948
HaeIII GGCC 4 cut(s) 5, 1334, 1973, 2017
HapII CCGG 1 cut(s) 38
HgaI GACGC 2 cut(s) 502, 2150
HindIII AAGCTT 1 cut(s) 716
HinfI GANTC 5 cut(s) 363, 567, 727, 1269, 1912
HpaII CCGG 1 cut(s) 38
HphI GGTGA 9 cut(s) 27, 296, 376, 915, 1402, 1438, 1477, 1518, 2180
Hpy166II GTNNAC 9 cut(s) 222, 566, 669, 1127, 1300, 1474, 1526, 1804, 2258
Hpy188I TCNGA 8 cut(s) 44, 356, 446, 723, 822, 1678, 1748, 2140
Hpy188III TCNNGA 8 cut(s) 506, 517, 1259, 1622, 1692, 1707, 1738, 1922
Hpy8I GTNNAC 9 cut(s) 222, 566, 669, 1127, 1300, 1474, 1526, 1804, 2258
Hpy99I CGWCG 2 cut(s) 518, 971
HpyAV CCTTC 7 cut(s) 65, 391, 653, 743, 947, 1274, 1713
HpyCH4III ACNGT 9 cut(s) 137, 238, 455, 583, 971, 1297, 1960, 2252, 2262
HpyCH4IV ACGT 2 cut(s) 10, 218
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 298, 327, 794, 1979, 2242
HpyF3I CTNAG 5 cut(s) 279, 529, 768, 798, 1870
HpySE526I ACGT 2 cut(s) 10, 218
Ksp22I TGATCA 2 cut(s) 130, 1372
Kzo9I GATC 4 cut(s) 130, 1020, 1372, 1611
LmnI GCTCC 2 cut(s) 1685, 1828
Lsp1109I GCAGC 6 cut(s) 92, 95, 110, 648, 816, 1646
LweI GCATC 6 cut(s) 6, 184, 279, 1856, 1979, 2232
MaeI CTAG 8 cut(s) 360, 561, 1185, 1730, 1754, 1823, 2181, 2193
MaeII ACGT 2 cut(s) 10, 218
MaeIII GTNAC 9 cut(s) 88, 182, 212, 259, 449, 497, 583, 845, 2127
MalI GATC 4 cut(s) 132, 1022, 1374, 1613
MboI GATC 4 cut(s) 130, 1020, 1372, 1611
MboII GAAGA 7 cut(s) 668, 784, 1241, 1564, 1910, 1913, 2117
MhlI GDGCHC 1 cut(s) 1737
MlsI TGGCCA 3 cut(s) 5, 1334, 1973
MluNI TGGCCA 3 cut(s) 5, 1334, 1973
MlyI GAGTC 3 cut(s) 357, 561, 1278
MmeI TCCRAC 4 cut(s) 22, 1060, 1182, 2025
Mox20I TGGCCA 3 cut(s) 5, 1334, 1973
Mph1103I ATGCAT 1 cut(s) 2212
MroXI GAANNNNTTC 2 cut(s) 407, 717
MscI TGGCCA 3 cut(s) 5, 1334, 1973
MseI TTAA 7 cut(s) 693, 732, 1035, 1052, 1383, 2085, 2133
MslI CAYNNNNRTG 5 cut(s) 481, 616, 969, 1419, 1623
Msp20I TGGCCA 3 cut(s) 5, 1334, 1973
MspCI CTTAAG 1 cut(s) 1034
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 3 cut(s) 120, 177, 927
Mva1269I GAATGC 1 cut(s) 1591
MvaI CCWGG 3 cut(s) 120, 177, 927
MwoI GCNNNNNNNGC 6 cut(s) 25, 298, 327, 794, 1979, 2242
NcoI CCATGG 2 cut(s) 1489, 1893
NdeI CATATG 2 cut(s) 494, 2212
NdeII GATC 4 cut(s) 130, 1020, 1372, 1611
NlaIV GGNNCC 4 cut(s) 250, 372, 1357, 1701
NmuCI GTSAC 3 cut(s) 497, 583, 2127
NsiI ATGCAT 1 cut(s) 2212
NspI RCATGY 4 cut(s) 490, 759, 915, 2224
OliI CACNNNNGTG 1 cut(s) 969
PaeI GCATGC 1 cut(s) 2224
PagI TCATGA 1 cut(s) 1258
PceI AGGCCT 1 cut(s) 2017
PciI ACATGT 2 cut(s) 755, 911
PcsI WCGNNNNNNNCGW 1 cut(s) 513
PctI GAATGC 1 cut(s) 1591
PdmI GAANNNNTTC 2 cut(s) 407, 717
PfeI GAWTC 2 cut(s) 727, 1912
PflFI GACNNNGTC 1 cut(s) 512
PflMI CCANNNNNTGG 1 cut(s) 2252
PinAI ACCGGT 1 cut(s) 37
PkrI GCNGC 6 cut(s) 82, 85, 125, 638, 806, 1636
PleI GAGTC 3 cut(s) 357, 561, 1277
PpsI GAGTC 3 cut(s) 357, 561, 1277
Ppu21I YACGTR 1 cut(s) 11
PscI ACATGT 2 cut(s) 755, 911
Psp6I CCWGG 3 cut(s) 118, 175, 925
PspEI GGTNACC 1 cut(s) 449
PspGI CCWGG 3 cut(s) 118, 175, 925
PspN4I GGNNCC 4 cut(s) 250, 372, 1357, 1701
PspPI GGNCC 1 cut(s) 822
PstI CTGCAG 2 cut(s) 1573, 2238
PsyI GACNNNGTC 1 cut(s) 512
RsaI GTAC 6 cut(s) 580, 1142, 1461, 2117, 2200, 2259
RsaNI GTAC 6 cut(s) 579, 1141, 1460, 2116, 2199, 2258
RseI CAYNNNNRTG 5 cut(s) 481, 616, 969, 1419, 1623
SaqAI TTAA 7 cut(s) 693, 732, 1035, 1052, 1383, 2085, 2133
SatI GCNGC 6 cut(s) 81, 84, 124, 637, 805, 1635
Sau3AI GATC 4 cut(s) 130, 1020, 1372, 1611
Sau96I GGNCC 1 cut(s) 822
ScaI AGTACT 2 cut(s) 1142, 2117
SchI GAGTC 3 cut(s) 357, 561, 1278
ScrFI CCNGG 3 cut(s) 120, 177, 927
SduI GDGCHC 1 cut(s) 1737
SfaNI GCATC 6 cut(s) 6, 184, 279, 1856, 1979, 2232
SfcI CTRYAG 2 cut(s) 1569, 2234
SinI GGWCC 1 cut(s) 822
SmiMI CAYNNNNRTG 5 cut(s) 481, 616, 969, 1419, 1623
SmlI CTYRAG 4 cut(s) 93, 1034, 1543, 1993
SmoI CTYRAG 4 cut(s) 93, 1034, 1543, 1993
SpeI ACTAGT 1 cut(s) 2192
SphI GCATGC 1 cut(s) 2224
SseBI AGGCCT 1 cut(s) 2017
SsiI CCGC 2 cut(s) 328, 2100
SspI AATATT 1 cut(s) 1642
SspMI CTAG 8 cut(s) 360, 561, 1185, 1730, 1754, 1823, 2181, 2193
StuI AGGCCT 1 cut(s) 2017
StyD4I CCNGG 3 cut(s) 118, 175, 925
StyI CCWWGG 4 cut(s) 1273, 1489, 1893, 2075
TaaI ACNGT 9 cut(s) 137, 238, 455, 583, 971, 1297, 1960, 2252, 2262
TaiI ACGT 2 cut(s) 13, 221
TaqI TCGA 3 cut(s) 507, 516, 1621
TatI WGTACW 2 cut(s) 1140, 2115
TfiI GAWTC 2 cut(s) 727, 1912
Tru1I TTAA 7 cut(s) 693, 732, 1035, 1052, 1383, 2085, 2133
Tru9I TTAA 7 cut(s) 693, 732, 1035, 1052, 1383, 2085, 2133
TscAI CASTG 6 cut(s) 133, 140, 229, 556, 715, 863
TseFI GTSAC 3 cut(s) 497, 583, 2127
TseI GCWGC 6 cut(s) 80, 83, 123, 636, 804, 1634
Tsp45I GTSAC 3 cut(s) 497, 583, 2127
TspDTI ATGAA 8 cut(s) 295, 949, 966, 1275, 1471, 1502, 1565, 2039
TspRI CASTG 6 cut(s) 133, 140, 229, 556, 715, 863
Tth111I GACNNNGTC 1 cut(s) 512
Van91I CCANNNNNTGG 1 cut(s) 2252
Vha464I CTTAAG 1 cut(s) 1034
VpaK11BI GGWCC 1 cut(s) 822
XapI RAATTY 2 cut(s) 919, 1083
XceI RCATGY 4 cut(s) 490, 759, 915, 2224
XcmI CCANNNNNNNNNTGG 1 cut(s) 240
XmiI GTMKAC 1 cut(s) 1473
XmnI GAANNNNTTC 2 cut(s) 407, 717
XspI CTAG 8 cut(s) 360, 561, 1185, 1730, 1754, 1823, 2181, 2193
ZrmI AGTACT 2 cut(s) 1142, 2117
Zsp2I ATGCAT 1 cut(s) 2212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.