RchiOBHm_Chr6g0306911

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000066
Physical Location & Seq
Reverse (-)
65429265 .. 65431782
2518 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27593

Sequence Viewer

Length: 2262 bp
ATGTCTATGAGGATAAAGAAAATGTTTTTGCTGCAATTGGTGCTGGCTGCAATAGAGGTGCTACTAGTATCATCCCAAACAGTGGATATTACAAGAGAGGGTTGCCAAGACAAATGTGGCAATGTTAGCATTCCCTACCCGTTCGGAACAAGAGGGGGTTGTTACCATGATGAGGATTTCCTCATTACTTGTAATAGCACAAGTTATAACCCTCCAAAGCCATTTCTGAGAAAGGGTAACATTGAGGTCACAAACATATCCATTGATGGTAAGTTGAATATCATGCAATACATAGCTCGAGATTGCTACGATTCATCTGGTTTCCGTATCAAGAAAAATTCTGCATCGACCCGCCTTTCAAAATTCATCATCTCCAACACCGATAATAAGTTCATTGCAGTTGGATGTGACACTTACGCAGGAATCCAAGGCTTCCAGGGCAAAATCATGAAGTACACAGGTGGCTGCATATCGACATGTGACAGCATTGATTATGTTGCCAATGATTCATGTTCCGTTATAGGGTGTTGCCAGACATCAATTGCTAAAGGTGTGAGCCATTTTGATGTGTTTTTGCACAGTTACCAGAATCATACCGATGTTTGGTCTTTCAATCCATGCAGCTTCGCCTTTATTGTGGAAGAAAGCAAGTTCAACTTCTCCAGAGCTATGCTCATGGATTTAAAGGATGTAACAGTACTTCCTGCTGTGCTTGATTGGTCAATTGGAAATGAGAATTGCAAGCAAGTTGAAGGCAAAATGATAGCTGGCAGCGCGTGCCAGGGGAACAGCACATGTGTTGATGTCGAAAATGGGTCTGGATACCGATGCAAGTGCAAGGATGGTTATCAAGGGAACCCGTACCTCTCTAATGGTTGTCAAGATATTGATGAATGTGAGGATCCAAAACTCAGTAACTGTGCACAAAAATGTGTAAATACAGAGGGAAATCATTACTGTTCTTGCCGCAAAGGGTACCATGGCAATGGTATAGCAGGGGAAGAAGGTTGCATAGCTAACAGAACATTAGTTGTTCAAATCACTGTTGGAATTGCTGTCGGCCTTATATCGTTGCTAACGTGTTGCTCTTGGCTATATCTCGGCTACAGAAGATGGAAACTCATGAAGCTGAAGGAGAGATTCTTCAGACAAAATGGAGGATTAATGTTACAACAACAACTTTCAGAACGACAAGGTGGTGCCACTAACCAAACTGCAAAAATCTTTACTGCAGAAGAACTTGAGAAGGCCACCAACAACTACCATGAGACTAGAATCGTTGGCAAAGGAGGCTACGGTACAGTGTACAAAGGAATTTTATCAGATGGAAGAGTAGTTGCAATCAAGAAATCCAAATTAGTTGATCAAAGCCAAATAGAGCAATTCATTAATGAAGTACTGGTGCTTTCCCAAATCAATCACAGGAATGTGGTGAAGCTTTTGGGTTGTTGTTTCGAGACAGAAGTCCCACGACTAGTTTATGAGTTTGTCACCAATGGAACGCTATTCGACTACATCCACAACAAAAGCAAGGCATCCAACTTTGCTTGGGAAACTCGTTTAAGGATAGCTGCAGAAGCTGCTGGAGTGCTATCATACTTGCACTCAGCTGCTTCTGTCCCTATTATCCATAGAGATGTCAAGTCCACAAATATACTTTTAGATGAAACCCTCACAGCAAAAGTGTCTGATTTTGGTGCCTCCAGATTGGTACCGTTGGATCAAACTGAGTTATCGACAATGGTACAAGGCACGCTCGGGTACTTAGACCCTGAATACTTTCAAACAAGCCAGTTGACTGACAAGAGTGATGTCTATAGCTTTGGGGTTGTCCTTGTGGAGCTACTAACAGGAAAGAAGGCGCTTTCGTTCGACAGGCAAGAGGAGGATAGAAATCTAGCAATGTATTTTCTTTCCGCACTGAAAGAAAACCGCTTGGTACAAGTGGTTGATGAGTGTGTTCTGAATGGGGCAAATGACAAGCAGCTCAAGGAAGTTTCTAACCTTGCGAAGAGGTGTTTGAAAGTGAAAGGAGAAGAAAGACCAACCATGATGGAAGTAGCAAGCGAGCTTGAGGGACTAAGAAGGATGGCAATGCATCATCCCTGGGTTAATAATGATGAATCCAATGCACAGGAGAATGAAAGCTTGCTTGGTGAAATATCACTGGAAACGTTTAGTCATGGAGGTGGTGGCGATACGAGTTCTGGGTATGATACAATGAGGAACCACATCATATTACCAGTAAATGATGGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase WAK

Protein Analysis

753

Amino Acids

83.55

Weight (kDa)

6.16

Isoelectric Point (pI)

30.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 90 1.5e-10 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 295 - 329 2.6e-06 Calcium-binding EGF domain
Pkinase PF00069 421 - 688 5.7e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 424 - 691 1.4e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 207
Acc65I GGTACC 2 cut(s) 975, 1711
AccB1I GGYRCC 4 cut(s) 975, 1199, 1697, 1711
AccB7I CCANNNNNTGG 1 cut(s) 82
AccII CGCG 1 cut(s) 776
AciI CCGC 4 cut(s) 352, 967, 1917, 1933
AclI AACGTT 1 cut(s) 2174
AclWI GGATC 3 cut(s) 896, 909, 1728
AcsI RAATTY 3 cut(s) 337, 362, 1314
AcuI CTGAAG 2 cut(s) 1129, 1151
AfiI CCNNNNNNNGG 4 cut(s) 82, 172, 522, 603
AflIII ACRYGT 3 cut(s) 476, 794, 1079
AgsI TTSAA 8 cut(s) 277, 360, 613, 655, 752, 1037, 1784, 2023
AhlI ACTAGT 2 cut(s) 64, 1474
AjnI CCWGG 3 cut(s) 435, 780, 2105
AjuI GAANNNNNNNTTGG 2 cut(s) 2136, 2168
Alw21I GWGCWC 1 cut(s) 925
Alw26I GTCTC 2 cut(s) 1262, 1451
Alw44I GTGCAC 1 cut(s) 921
AlwI GGATC 3 cut(s) 896, 909, 1728
AlwNI CAGNNNCTG 2 cut(s) 918, 1580
Ama87I CYCGRG 2 cut(s) 297, 1757
AoxI GGCC 2 cut(s) 1060, 1248
ApaLI GTGCAC 1 cut(s) 921
ApeKI GCWGC 9 cut(s) 31, 47, 465, 621, 771, 1571, 1580, 1610, 1984
ApoI RAATTY 3 cut(s) 337, 362, 1314
AseI ATTAAT 2 cut(s) 1163, 1389
Asp700I GAANNNNTTC 1 cut(s) 1779
Asp718I GGTACC 2 cut(s) 975, 1711
AspLEI GCGC 2 cut(s) 776, 1864
AsuHPI GGTGA 3 cut(s) 1444, 1483, 2168
AvaI CYCGRG 2 cut(s) 297, 1757
BaeGI GKGCMC 1 cut(s) 925
BamHI GGATCC 1 cut(s) 901
BanI GGYRCC 4 cut(s) 975, 1199, 1697, 1711
BarI GAAGNNNNNNTAC 2 cut(s) 684, 716
Bbv12I GWGCWC 1 cut(s) 925
BbvI GCAGC 9 cut(s) 18, 34, 452, 633, 783, 1558, 1567, 1597, 1996
BccI CCATC 7 cut(s) 260, 836, 1107, 1319, 2047, 2083, 2246
BcgI CGANNNNNNTGC 2 cut(s) 816, 850
BciT130I CCWGG 3 cut(s) 437, 782, 2107
BciVI GTATCC 1 cut(s) 815
BclI TGATCA 1 cut(s) 1363
BcoDI GTCTC 2 cut(s) 1262, 1451
BcuI ACTAGT 2 cut(s) 64, 1474
BfaI CTAG 4 cut(s) 65, 1272, 1475, 1898
BfmI CTRYAG 4 cut(s) 1105, 1230, 1572, 1816
BfoI RGCGCY 1 cut(s) 1865
BfuI GTATCC 1 cut(s) 815
BmcAI AGTACT 2 cut(s) 699, 1398
Bme1390I CCNGG 3 cut(s) 437, 782, 2107
BmeT110I CYCGRG 2 cut(s) 297, 1757
BmiI GGNNCC 7 cut(s) 857, 903, 977, 1201, 1699, 1713, 2228
BmrFI CCNGG 3 cut(s) 437, 782, 2107
BmsI GCATC 4 cut(s) 353, 818, 1544, 2107
BoxI GACNNNNGTC 1 cut(s) 1463
BplI GAGNNNNNCTC 2 cut(s) 657, 689
BpmI CTGGAG 3 cut(s) 646, 1605, 1687
BpuEI CTTGAG 3 cut(s) 1262, 1973, 2093
BsaBI GATNNNNATC 2 cut(s) 846, 1893
BsaJI CCNNGG 6 cut(s) 427, 436, 781, 979, 2105, 2106
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 172, 522, 603
Bse1I ACTGG 4 cut(s) 1404, 1792, 2172, 2243
Bse3DI GCAATG 5 cut(s) 127, 393, 991, 1908, 2100
Bse8I GATNNNNATC 2 cut(s) 846, 1893
BseBI CCWGG 3 cut(s) 437, 782, 2107
BseDI CCNNGG 6 cut(s) 427, 436, 781, 979, 2105, 2106
BseGI GGATG 8 cut(s) 71, 410, 694, 847, 1515, 1535, 2094, 2101
BseJI GATNNNNATC 2 cut(s) 846, 1893
BseLI CCNNNNNNNGG 4 cut(s) 82, 172, 522, 603
BseMI GCAATG 5 cut(s) 127, 393, 991, 1908, 2100
BseMII CTCAG 4 cut(s) 218, 925, 1620, 1719
BseNI ACTGG 4 cut(s) 1404, 1792, 2172, 2243
BseRI GAGGAG 1 cut(s) 1898
BseSI GKGCMC 1 cut(s) 925
BseXI GCAGC 9 cut(s) 18, 34, 452, 633, 783, 1558, 1567, 1597, 1996
Bsh1236I CGCG 1 cut(s) 776
BshFI GGCC 2 cut(s) 1062, 1250
BshNI GGYRCC 4 cut(s) 975, 1199, 1697, 1711
BsiHKAI GWGCWC 1 cut(s) 925
BsiHKCI CYCGRG 2 cut(s) 297, 1757
BslFI GGGAC 3 cut(s) 1451, 1604, 2091
BslI CCNNNNNNNGG 4 cut(s) 82, 172, 522, 603
BsmAI GTCTC 2 cut(s) 1262, 1451
BsmFI GGGAC 3 cut(s) 1451, 1604, 2091
BsmI GAATGC 1 cut(s) 129
BsnI GGCC 2 cut(s) 1062, 1250
BsoBI CYCGRG 2 cut(s) 297, 1757
Bsp1286I GDGCHC 1 cut(s) 925
Bsp1407I TGTACA 1 cut(s) 1305
Bsp143I GATC 3 cut(s) 901, 1363, 1720
Bsp19I CCATGG 1 cut(s) 979
BspACI CCGC 4 cut(s) 352, 967, 1917, 1933
BspANI GGCC 2 cut(s) 1062, 1250
BspCNI CTCAG 4 cut(s) 219, 924, 1619, 1720
BspFNI CGCG 1 cut(s) 776
BspHI TCATGA 2 cut(s) 447, 1122
BspLI GGNNCC 7 cut(s) 857, 903, 977, 1201, 1699, 1713, 2228
BspMAI CTGCAG 2 cut(s) 1234, 1576
BspPI GGATC 3 cut(s) 896, 909, 1728
BspT107I GGYRCC 4 cut(s) 975, 1199, 1697, 1711
BsrDI GCAATG 5 cut(s) 127, 393, 991, 1908, 2100
BsrGI TGTACA 1 cut(s) 1305
BsrI ACTGG 4 cut(s) 1404, 1792, 2172, 2243
BssECI CCNNGG 6 cut(s) 427, 436, 781, 979, 2105, 2106
BssMI GATC 3 cut(s) 901, 1363, 1720
BssT1I CCWWGG 2 cut(s) 427, 979
Bst2UI CCWGG 3 cut(s) 437, 782, 2107
Bst4CI ACNGT 9 cut(s) 82, 581, 697, 920, 959, 1045, 1298, 1303, 1716
Bst6I CTCTTC 2 cut(s) 1324, 2006
BstAPI GCANNNNNTGC 3 cut(s) 40, 777, 1580
BstAUI TGTACA 1 cut(s) 1305
BstC8I GCNNGC 8 cut(s) 45, 743, 769, 778, 1754, 2065, 2069, 2150
BstDEI CTNAG 6 cut(s) 227, 911, 1606, 1728, 1765, 2081
BstDSI CCRYGG 1 cut(s) 979
BstF5I GGATG 8 cut(s) 71, 410, 694, 847, 1515, 1535, 2094, 2101
BstFNI CGCG 1 cut(s) 776
BstH2I RGCGCY 1 cut(s) 1865
BstHHI GCGC 2 cut(s) 776, 1864
BstKTI GATC 3 cut(s) 904, 1366, 1723
BstMAI GTCTC 2 cut(s) 1262, 1451
BstMBI GATC 3 cut(s) 901, 1363, 1720
BstMWI GCNNNNNNNGC 8 cut(s) 40, 126, 438, 773, 777, 1290, 1577, 1580
BstNI CCWGG 3 cut(s) 437, 782, 2107
BstNSI RCATGY 2 cut(s) 480, 798
BstPAI GACNNNNGTC 1 cut(s) 1463
BstSCI CCNGG 3 cut(s) 435, 780, 2105
BstSFI CTRYAG 4 cut(s) 1105, 1230, 1572, 1816
BstSLI GKGCMC 1 cut(s) 925
BstUI CGCG 1 cut(s) 776
BstV1I GCAGC 9 cut(s) 18, 34, 452, 633, 783, 1558, 1567, 1597, 1996
BstX2I RGATCY 1 cut(s) 901
BstXI CCANNNNNNTGG 1 cut(s) 986
BstYI RGATCY 1 cut(s) 901
BsuI GTATCC 1 cut(s) 815
BsuRI GGCC 2 cut(s) 1062, 1250
BtgI CCRYGG 1 cut(s) 979
BtsCI GGATG 8 cut(s) 71, 410, 694, 847, 1515, 1535, 2094, 2101
BtsIMutI CAGTG 5 cut(s) 87, 1041, 1308, 1919, 2165
Cac8I GCNNGC 8 cut(s) 45, 743, 769, 778, 1754, 2065, 2069, 2150
CaiI CAGNNNCTG 2 cut(s) 918, 1580
CciI TCATGA 2 cut(s) 447, 1122
CfoI GCGC 2 cut(s) 776, 1864
DdeI CTNAG 6 cut(s) 227, 911, 1606, 1728, 1765, 2081
DpnI GATC 3 cut(s) 903, 1365, 1722
DpnII GATC 3 cut(s) 901, 1363, 1720
DraI TTTAAA 1 cut(s) 684
Eam1104I CTCTTC 2 cut(s) 1324, 2006
EarI CTCTTC 2 cut(s) 1324, 2006
Eco130I CCWWGG 2 cut(s) 427, 979
Eco57I CTGAAG 2 cut(s) 1129, 1151
Eco88I CYCGRG 2 cut(s) 297, 1757
EcoRII CCWGG 3 cut(s) 435, 780, 2105
EcoT14I CCWWGG 2 cut(s) 427, 979
EcoT22I ATGCAT 1 cut(s) 2100
ErhI CCWWGG 2 cut(s) 427, 979
FalI AAGNNNNNCTT 2 cut(s) 641, 673
FaqI GGGAC 3 cut(s) 1451, 1604, 2091
FauI CCCGC 1 cut(s) 359
FbaI TGATCA 1 cut(s) 1363
FokI GGATG 8 cut(s) 58, 417, 701, 854, 1502, 1522, 2088, 2101
FspBI CTAG 4 cut(s) 65, 1272, 1475, 1898
GlaI GCGC 2 cut(s) 775, 1863
GsuI CTGGAG 3 cut(s) 646, 1605, 1687
HaeII RGCGCY 1 cut(s) 1865
HaeIII GGCC 2 cut(s) 1062, 1250
HhaI GCGC 2 cut(s) 776, 1864
Hin6I GCGC 2 cut(s) 774, 1862
HinP1I GCGC 2 cut(s) 774, 1862
HincII GTYRAC 1 cut(s) 1797
HindII GTYRAC 1 cut(s) 1797
HindIII AAGCTT 2 cut(s) 1436, 2146
HinfI GANTC 7 cut(s) 311, 423, 506, 589, 1140, 1275, 2123
HphI GGTGA 3 cut(s) 1444, 1483, 2168
Hpy166II GTNNAC 5 cut(s) 456, 923, 1306, 1647, 1797
Hpy188I TCNGA 7 cut(s) 146, 228, 1148, 1186, 1324, 1690, 1965
Hpy8I GTNNAC 5 cut(s) 456, 923, 1306, 1647, 1797
HpyAV CCTTC 6 cut(s) 746, 998, 1126, 1240, 1852, 2079
HpyCH4III ACNGT 9 cut(s) 82, 581, 697, 920, 959, 1045, 1298, 1303, 1716
HpyCH4IV ACGT 2 cut(s) 1079, 2174
HpyF10VI GCNNNNNNNGC 8 cut(s) 40, 126, 438, 773, 777, 1290, 1577, 1580
HpyF3I CTNAG 6 cut(s) 227, 911, 1606, 1728, 1765, 2081
HpySE526I ACGT 2 cut(s) 1079, 2174
HspAI GCGC 2 cut(s) 774, 1862
KpnI GGTACC 2 cut(s) 979, 1715
Ksp22I TGATCA 1 cut(s) 1363
Kzo9I GATC 3 cut(s) 901, 1363, 1720
LmnI GCTCC 1 cut(s) 1840
Lsp1109I GCAGC 9 cut(s) 18, 34, 452, 633, 783, 1558, 1567, 1597, 1996
LweI GCATC 4 cut(s) 353, 818, 1544, 2107
MaeI CTAG 4 cut(s) 65, 1272, 1475, 1898
MaeII ACGT 2 cut(s) 1079, 2174
MalI GATC 3 cut(s) 903, 1365, 1722
MboI GATC 3 cut(s) 901, 1363, 1720
MboII GAAGA 8 cut(s) 653, 1013, 1122, 1135, 1247, 1341, 2023, 2048
MfeI CAATTG 3 cut(s) 35, 540, 723
MflI RGATCY 1 cut(s) 901
MhlI GDGCHC 1 cut(s) 925
MmeI TCCRAC 5 cut(s) 382, 399, 1027, 1563, 1698
Mph1103I ATGCAT 1 cut(s) 2100
MroXI GAANNNNTTC 1 cut(s) 1779
MseI TTAA 5 cut(s) 683, 1163, 1389, 1562, 2112
MslI CAYNNNNRTG 7 cut(s) 564, 597, 928, 984, 1425, 1635, 2187
MspA1I CMGCKG 1 cut(s) 1610
MspR9I CCNGG 3 cut(s) 437, 782, 2107
MunI CAATTG 3 cut(s) 35, 540, 723
Mva1269I GAATGC 1 cut(s) 129
MvaI CCWGG 3 cut(s) 437, 782, 2107
MvnI CGCG 1 cut(s) 776
MwoI GCNNNNNNNGC 8 cut(s) 40, 126, 438, 773, 777, 1290, 1577, 1580
NcoI CCATGG 1 cut(s) 979
NdeII GATC 3 cut(s) 901, 1363, 1720
NlaIV GGNNCC 7 cut(s) 857, 903, 977, 1201, 1699, 1713, 2228
NmeAIII GCCGAG 1 cut(s) 1080
NmuCI GTSAC 4 cut(s) 247, 407, 479, 1489
NsiI ATGCAT 1 cut(s) 2100
NspI RCATGY 2 cut(s) 480, 798
PaeR7I CTCGAG 1 cut(s) 297
PagI TCATGA 2 cut(s) 447, 1122
PasI CCCWGGG 1 cut(s) 2106
PciI ACATGT 2 cut(s) 476, 794
PcsI WCGNNNNNNNCGW 1 cut(s) 1076
PctI GAATGC 1 cut(s) 129
PdmI GAANNNNTTC 1 cut(s) 1779
PfeI GAWTC 7 cut(s) 311, 423, 506, 589, 1140, 1275, 2123
PflMI CCANNNNNTGG 1 cut(s) 82
PscI ACATGT 2 cut(s) 476, 794
PshAI GACNNNNGTC 1 cut(s) 1463
PshBI ATTAAT 2 cut(s) 1163, 1389
PsiI TTATAA 1 cut(s) 207
Psp1406I AACGTT 1 cut(s) 2174
Psp6I CCWGG 3 cut(s) 435, 780, 2105
PspGI CCWGG 3 cut(s) 435, 780, 2105
PspN4I GGNNCC 7 cut(s) 857, 903, 977, 1201, 1699, 1713, 2228
PstI CTGCAG 2 cut(s) 1234, 1576
PstNI CAGNNNCTG 2 cut(s) 918, 1580
PsuI RGATCY 1 cut(s) 901
PvuII CAGCTG 1 cut(s) 1610
RseI CAYNNNNRTG 7 cut(s) 564, 597, 928, 984, 1425, 1635, 2187
SaqAI TTAA 5 cut(s) 683, 1163, 1389, 1562, 2112
Sau3AI GATC 3 cut(s) 901, 1363, 1720
ScaI AGTACT 2 cut(s) 699, 1398
ScrFI CCNGG 3 cut(s) 437, 782, 2107
SduI GDGCHC 1 cut(s) 925
SfaNI GCATC 4 cut(s) 353, 818, 1544, 2107
SfcI CTRYAG 4 cut(s) 1105, 1230, 1572, 1816
Sfr274I CTCGAG 1 cut(s) 297
SlaI CTCGAG 1 cut(s) 297
SmiMI CAYNNNNRTG 7 cut(s) 564, 597, 928, 984, 1425, 1635, 2187
SmlI CTYRAG 4 cut(s) 297, 1241, 1988, 2072
SmoI CTYRAG 4 cut(s) 297, 1241, 1988, 2072
SpeI ACTAGT 2 cut(s) 64, 1474
SsiI CCGC 4 cut(s) 352, 967, 1917, 1933
SspMI CTAG 4 cut(s) 65, 1272, 1475, 1898
StyD4I CCNGG 3 cut(s) 435, 780, 2105
StyI CCWWGG 2 cut(s) 427, 979
TaaI ACNGT 9 cut(s) 82, 581, 697, 920, 959, 1045, 1298, 1303, 1716
TaiI ACGT 2 cut(s) 1082, 2177
TaqI TCGA 8 cut(s) 298, 347, 473, 807, 1455, 1509, 1736, 1872
TatI WGTACW 4 cut(s) 453, 697, 1305, 1396
TauI GCSGC 1 cut(s) 969
TfiI GAWTC 7 cut(s) 311, 423, 506, 589, 1140, 1275, 2123
Tru1I TTAA 5 cut(s) 683, 1163, 1389, 1562, 2112
Tru9I TTAA 5 cut(s) 683, 1163, 1389, 1562, 2112
TscAI CASTG 5 cut(s) 87, 1048, 1308, 1926, 2172
TseFI GTSAC 4 cut(s) 247, 407, 479, 1489
TseI GCWGC 9 cut(s) 31, 47, 465, 621, 771, 1571, 1580, 1610, 1984
Tsp45I GTSAC 4 cut(s) 247, 407, 479, 1489
TspGWI ACGGA 2 cut(s) 314, 505
TspRI CASTG 5 cut(s) 87, 1048, 1308, 1926, 2172
Van91I CCANNNNNTGG 1 cut(s) 82
VneI GTGCAC 1 cut(s) 921
VspI ATTAAT 2 cut(s) 1163, 1389
XapI RAATTY 3 cut(s) 337, 362, 1314
XceI RCATGY 2 cut(s) 480, 798
XcmI CCANNNNNNNNNTGG 1 cut(s) 113
XhoI CTCGAG 1 cut(s) 297
XmnI GAANNNNTTC 1 cut(s) 1779
XspI CTAG 4 cut(s) 65, 1272, 1475, 1898
ZrmI AGTACT 2 cut(s) 699, 1398
Zsp2I ATGCAT 1 cut(s) 2100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.