RchiOBHm_Chr5g0017141

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000066
Physical Location & Seq
Reverse (-)
11873515 .. 11876363
2849 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29746

Sequence Viewer

Length: 2268 bp
ATGTCGATCATGGCCTTGCAATTCCCATTGGCGGCAGTCCTAATCCTATTATCGGCGGCTACTACTACGACAACAGCAGCTGCTGTTCAAGCCCTGCCTGGCTGCTCTGACAAGTGCGGTGATCTCACAATTCCATATCCATTTGGCATGAATGAGAGTTGTTACCTGGGAGATATGTTCCGCATCAACTGTAGCCAGGGGGCCCAACCGGACCAACCCCCAACAGCCTACTTGGGGGACACCAATATCATTGTTACCAGCATATCCATAGACGTTGGGGAGTTGCGAATATTGAGCCATAGAGGGGAAGATTGTTACAATGAAACGGGCAATCTAACTTACCGAAACAGACCTCATTTCTGGATGGGTCCTCCATACACCATATCTGATACCAAAAACAAGTTCTATGCCGTTGGTTGTGACACTTACGCAATCATGCGAGGCTTCCGAGGGGAAGAAGAATTCATTACTGGGTGCGTGTCAGTATGCAACAGCTTTAGCAGTGTTGATAAGAACTCTTGCTCTGGCGCTGGGTGTTGCCAAACTAGCATCCCCAGTGGACTGCACAATCGTACTGTGAGTTTGGATAGCTACTTCAATCATTCGTATGTGTGGGACTTTAACCCCTGCAGCTACGCCTTCATTGCAGAGCAAGGCCAATTCAATTTCTCCTCTTCCAGTTTTGAACAACTGAATGACACTGAACGACTTCCAATGGTCCTTAATTGGGCCATTGGGAATGACACAGACCCCTGTCATGAAGCTCAGAAAAGGGAGGATTTCGCATGCACAGCTAATAGCATATGCAAAAACAGGCCTATCAATGGGTCTGTAGGTTATTTGTGCGAGTGTTCGCCTGGTTATGAAGGAAACCCGTACCACCCAGATGGTTGCAAAGATATTGACGAGTGCAAGGCTTTGCCCTGCGAGAACGGACAGTGCTTCAATAAACCATTTCCAGGAAATTACTCTTGTTCATGTTACAAAGGATACAAAAACGACGGCATGAATGACAAGATTTGCATCAAAGACGATTCCAAAAGACACTCAAAGATCGTTCTCCTGCTTATGATTTCATTGGGTGTAAGTATGGGCTTCTTGGTTTTATTCCCGTGGATATGTTGGGGAATGAAGAAAAGAGAGTTCATTAAACTCAAAGAAAAGTACTTCAAAGAGAATGGTGGCTTATTGTTTCTGCAACAACTCGCTAGTCATGGAAGCTCGATGAAGACAACAAAAATCTTTACTGCTGAAGAACTTGAGAAGGCAACAAACAACTATCATGAGAGTAGAGTCCTTGGAGAAGGAGGTTATGGAACAGTTTACAAAGGTATACTGGCGGATGACATAGTGGTTGCCATAAAGAAGTCGAAAGGTGGTGCCCTGACCCAGAGTGATCAGTTTGTTAACGAGGTGATTGTCCTTTCTCAAATCAACCACAGAAATGTGGTAAAGCTATTAGGTTGTTGTTTAGAAACAGAAGTGCCTCTACTAGTATACGAATTCATTACACATGGCACTCTTTATGAGCACATTCATAAAAAAGGATCATCACTCCCATTTGAATTACGAATGAAGATAGCAGCTCAAAGTGCGGAAGCGCTGTCCCACTTACACTCCTCAATTTCCACACCAATCATACATCGAGATGTCAAAGCAGCGAATATTCTGTTAGATGATAAGTATACGGCGAAAGTGTCAGACTTTGGAGCTTCACGATTGGTTTCTTCAGGTGAAGCTGGAATACGAACTGTAGTGCTCGGGACAATAGGATACCTGGACCCTGAATATCTGCAATCAAACCAACTAACAGAAAAGAGTGACGTTTACAGCTTTGGGGTTCTCTTGGCTGAGCTTTTAACAAGCAAAGTGGCAGTTTCTAAAGATAAATTCTTAGCAAGCATCTTTGTTGCTTCCATGGAAAAAGATTGCTTAAATCAAGTGCTTGATGATGAGATAGTGAATGAAGGAAATATTGAGATGGCAAAAAATGTGGCCAAACTTGCAAGAAGATGTTTGAGGGTAAAAGGGGAGGAAAGGCCTACCATGAAAGAAGTAGCCATGGAGCTAGAAGGATTGATTATGACAAAGCATCCATGGGGTTCTGCAGATCATAACTTTCCAGAAGAGACTAAGCACTTGCTCGGATCACCTAATACTTCCAAGGATTACTCTGTGAATGTTGATGGTGGTGGTGGTCCTGGTACTGCCAGTGGGTCCAGCATGCAAATAGACATGTCAATGTCTTCGTATGCTGATGGGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

755

Amino Acids

83.0

Weight (kDa)

5.69

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 97 8.2e-09 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 427 - 686 2.4e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 430 - 690 1.8e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1379
AccI GTMKAC 3 cut(s) 1333, 1497, 1685
AciI CCGC 6 cut(s) 32, 56, 117, 181, 1340, 1595
AclWI GGATC 2 cut(s) 1555, 2155
AcoI YGGCCR 1 cut(s) 1995
AcsI RAATTY 3 cut(s) 461, 1502, 1889
AcuI CTGAAG 2 cut(s) 1272, 1713
AfaI GTAC 4 cut(s) 574, 878, 1166, 2206
AfeI AGCGCT 1 cut(s) 1602
AfiI CCNNNNNNNGG 7 cut(s) 31, 52, 234, 304, 727, 824, 959
AflIII ACRYGT 1 cut(s) 2235
AgsI TTSAA 7 cut(s) 89, 598, 664, 686, 946, 1171, 1565
AhlI ACTAGT 1 cut(s) 1492
AjnI CCWGG 7 cut(s) 97, 165, 195, 856, 958, 1776, 2200
AjuI GAANNNNNNNTTGG 2 cut(s) 386, 418
Alw21I GWGCWC 2 cut(s) 1533, 1761
Alw26I GTCTC 1 cut(s) 2123
AlwI GGATC 2 cut(s) 1555, 2155
AlwNI CAGNNNCTG 2 cut(s) 80, 83
Ama87I CYCGRG 1 cut(s) 1760
Aor51HI AGCGCT 1 cut(s) 1602
AoxI GGCC 7 cut(s) 12, 201, 655, 729, 815, 1995, 2039
ApaI GGGCCC 1 cut(s) 205
ApeKI GCWGC 6 cut(s) 77, 80, 102, 630, 1583, 1658
ApoI RAATTY 3 cut(s) 461, 1502, 1889
Asp700I GAANNNNTTC 1 cut(s) 708
AspLEI GCGC 2 cut(s) 530, 1603
AspS9I GGNCC 9 cut(s) 201, 202, 211, 368, 718, 729, 1780, 2198, 2217
AsuHPI GGTGA 4 cut(s) 131, 1426, 1745, 2142
AvaI CYCGRG 1 cut(s) 1760
AvaII GGWCC 6 cut(s) 211, 368, 718, 1780, 2198, 2217
BaeGI GKGCMC 2 cut(s) 205, 1384
BalI TGGCCA 1 cut(s) 1997
BanI GGYRCC 1 cut(s) 1379
BanII GRGCYC 1 cut(s) 205
BarI GAAGNNNNNNTAC 4 cut(s) 1473, 1505, 1728, 1760
BbsI GAAGAC 2 cut(s) 1235, 2238
Bbv12I GWGCWC 2 cut(s) 1533, 1761
BbvI GCAGC 6 cut(s) 67, 89, 89, 642, 1595, 1670
BccI CCATC 5 cut(s) 358, 881, 1975, 2180, 2252
BceAI ACGGC 3 cut(s) 395, 1018, 1704
BciT130I CCWGG 7 cut(s) 99, 167, 197, 858, 960, 1778, 2202
BciVI GTATCC 2 cut(s) 983, 1766
BclI TGATCA 1 cut(s) 1396
BcoDI GTCTC 1 cut(s) 2123
BcuI ACTAGT 1 cut(s) 1492
BfaI CTAG 4 cut(s) 546, 1209, 1493, 2069
BfmI CTRYAG 5 cut(s) 190, 628, 831, 1752, 2106
BfoI RGCGCY 2 cut(s) 531, 1604
BfuI GTATCC 2 cut(s) 983, 1766
BisI GCNGC 8 cut(s) 33, 57, 78, 81, 103, 631, 1584, 1659
BlpI GCTNAGC 1 cut(s) 1851
BlsI GCNGC 8 cut(s) 34, 58, 79, 82, 104, 632, 1585, 1660
BmcAI AGTACT 1 cut(s) 1166
Bme1390I CCNGG 7 cut(s) 99, 167, 197, 858, 960, 1778, 2202
Bme18I GGWCC 6 cut(s) 211, 368, 718, 1780, 2198, 2217
BmeT110I CYCGRG 1 cut(s) 1760
BmgT120I GGNCC 9 cut(s) 201, 202, 211, 368, 718, 729, 1780, 2198, 2217
BmiI GGNNCC 6 cut(s) 202, 203, 369, 1381, 1782, 2218
BmrFI CCNGG 7 cut(s) 99, 167, 197, 858, 960, 1778, 2202
BmrI ACTGGG 2 cut(s) 480, 549
BmsI GCATC 5 cut(s) 192, 558, 1032, 1911, 2101
BmuI ACTGGG 2 cut(s) 480, 549
BoxI GACNNNNGTC 1 cut(s) 753
BpiI GAAGAC 2 cut(s) 1235, 2238
Bpu1102I GCTNAGC 1 cut(s) 1851
BpuEI CTTGAG 1 cut(s) 1280
BsaBI GATNNNNATC 1 cut(s) 1022
BsaJI CCNNGG 9 cut(s) 166, 196, 448, 1112, 1297, 1917, 2061, 2096, 2163
BsaWI WCCGGW 1 cut(s) 208
BsaXI ACNNNNNCTCC 2 cut(s) 1601, 1631
Bsc4I CCNNNNNNNGG 7 cut(s) 31, 52, 234, 304, 727, 824, 959
Bse1I ACTGG 5 cut(s) 475, 555, 678, 1341, 2211
Bse3DI GCAATG 1 cut(s) 642
Bse8I GATNNNNATC 1 cut(s) 1022
BseBI CCWGG 7 cut(s) 99, 167, 197, 858, 960, 1778, 2202
BseDI CCNNGG 9 cut(s) 166, 196, 448, 1112, 1297, 1917, 2061, 2096, 2163
BseGI GGATG 4 cut(s) 369, 549, 1348, 2092
BseJI GATNNNNATC 1 cut(s) 1022
BseLI CCNNNNNNNGG 7 cut(s) 31, 52, 234, 304, 727, 824, 959
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 2 cut(s) 779, 1842
BseNI ACTGG 5 cut(s) 475, 555, 678, 1341, 2211
BseRI GAGGAG 2 cut(s) 661, 1609
BseSI GKGCMC 2 cut(s) 205, 1384
BseXI GCAGC 6 cut(s) 67, 89, 89, 642, 1595, 1670
BseYI CCCAGC 1 cut(s) 530
BsgI GTGCAG 1 cut(s) 548
BshFI GGCC 7 cut(s) 14, 203, 657, 731, 817, 1997, 2041
BshNI GGYRCC 1 cut(s) 1379
BsiHKAI GWGCWC 2 cut(s) 1533, 1761
BsiHKCI CYCGRG 1 cut(s) 1760
BsiSI CCGG 1 cut(s) 209
BslFI GGGAC 4 cut(s) 251, 629, 1591, 1777
BslI CCNNNNNNNGG 7 cut(s) 31, 52, 234, 304, 727, 824, 959
BsmAI GTCTC 1 cut(s) 2123
BsmFI GGGAC 4 cut(s) 251, 629, 1591, 1777
BsnI GGCC 7 cut(s) 14, 203, 657, 731, 817, 1997, 2041
BsoBI CYCGRG 1 cut(s) 1760
Bsp120I GGGCCC 1 cut(s) 201
Bsp1286I GDGCHC 4 cut(s) 205, 1384, 1533, 1761
Bsp143I GATC 7 cut(s) 6, 121, 1053, 1396, 1547, 2110, 2147
Bsp1720I GCTNAGC 1 cut(s) 1851
Bsp19I CCATGG 3 cut(s) 1917, 2061, 2096
BspACI CCGC 6 cut(s) 32, 56, 117, 181, 1340, 1595
BspANI GGCC 7 cut(s) 14, 203, 657, 731, 817, 1997, 2041
BspCNI CTCAG 2 cut(s) 778, 1843
BspHI TCATGA 2 cut(s) 757, 1282
BspLI GGNNCC 6 cut(s) 202, 203, 369, 1381, 1782, 2218
BspMAI CTGCAG 2 cut(s) 632, 2110
BspPI GGATC 2 cut(s) 1555, 2155
BspT107I GGYRCC 1 cut(s) 1379
BsrDI GCAATG 1 cut(s) 642
BsrI ACTGG 5 cut(s) 475, 555, 678, 1341, 2211
BssECI CCNNGG 9 cut(s) 166, 196, 448, 1112, 1297, 1917, 2061, 2096, 2163
BssMI GATC 7 cut(s) 6, 121, 1053, 1396, 1547, 2110, 2147
BssNAI GTATAC 3 cut(s) 1334, 1498, 1686
BssT1I CCWWGG 5 cut(s) 1297, 1917, 2061, 2096, 2163
Bst1107I GTATAC 3 cut(s) 1334, 1498, 1686
Bst2UI CCWGG 7 cut(s) 99, 167, 197, 858, 960, 1778, 2202
Bst4CI ACNGT 5 cut(s) 191, 577, 939, 1321, 1753
Bst6I CTCTTC 2 cut(s) 679, 2121
BstC8I GCNNGC 3 cut(s) 787, 1900, 2225
BstDEI CTNAG 4 cut(s) 765, 1851, 1894, 2133
BstDSI CCRYGG 4 cut(s) 1112, 1917, 2061, 2096
BstF5I GGATG 4 cut(s) 369, 549, 1348, 2092
BstH2I RGCGCY 2 cut(s) 531, 1604
BstHHI GCGC 2 cut(s) 530, 1603
BstKTI GATC 7 cut(s) 9, 124, 1056, 1399, 1550, 2113, 2150
BstMAI GTCTC 1 cut(s) 2123
BstMBI GATC 7 cut(s) 6, 121, 1053, 1396, 1547, 2110, 2147
BstMWI GCNNNNNNNGC 6 cut(s) 89, 546, 644, 791, 1592, 2003
BstNI CCWGG 7 cut(s) 99, 167, 197, 858, 960, 1778, 2202
BstNSI RCATGY 3 cut(s) 789, 2227, 2239
BstPAI GACNNNNGTC 1 cut(s) 753
BstSCI CCNGG 7 cut(s) 97, 165, 195, 856, 958, 1776, 2200
BstSFI CTRYAG 5 cut(s) 190, 628, 831, 1752, 2106
BstSLI GKGCMC 2 cut(s) 205, 1384
BstV1I GCAGC 6 cut(s) 67, 89, 89, 642, 1595, 1670
BstV2I GAAGAC 2 cut(s) 1235, 2238
BstXI CCANNNNNNTGG 1 cut(s) 887
BstZ17I GTATAC 3 cut(s) 1334, 1498, 1686
BsuI GTATCC 2 cut(s) 983, 1766
BsuRI GGCC 7 cut(s) 14, 203, 657, 731, 817, 1997, 2041
BtgI CCRYGG 4 cut(s) 1112, 1917, 2061, 2096
BtsCI GGATG 4 cut(s) 369, 549, 1348, 2092
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 5 cut(s) 508, 562, 699, 944, 2218
Cac8I GCNNGC 3 cut(s) 787, 1900, 2225
CaiI CAGNNNCTG 2 cut(s) 80, 83
CciI TCATGA 2 cut(s) 757, 1282
CfoI GCGC 2 cut(s) 530, 1603
Cfr13I GGNCC 9 cut(s) 201, 202, 211, 368, 718, 729, 1780, 2198, 2217
Csp6I GTAC 4 cut(s) 573, 877, 1165, 2205
CspCI CAANNNNNGTGG 4 cut(s) 1851, 1886, 1974, 2009
CviQI GTAC 4 cut(s) 573, 877, 1165, 2205
DdeI CTNAG 4 cut(s) 765, 1851, 1894, 2133
DpnI GATC 7 cut(s) 8, 123, 1055, 1398, 1549, 2112, 2149
DpnII GATC 7 cut(s) 6, 121, 1053, 1396, 1547, 2110, 2147
EaeI YGGCCR 1 cut(s) 1995
Eam1104I CTCTTC 2 cut(s) 679, 2121
EarI CTCTTC 2 cut(s) 679, 2121
EciI GGCGGA 1 cut(s) 1355
Eco130I CCWWGG 5 cut(s) 1297, 1917, 2061, 2096, 2163
Eco147I AGGCCT 2 cut(s) 817, 2041
Eco24I GRGCYC 1 cut(s) 205
Eco47I GGWCC 6 cut(s) 211, 368, 718, 1780, 2198, 2217
Eco47III AGCGCT 1 cut(s) 1602
Eco57I CTGAAG 2 cut(s) 1272, 1713
Eco88I CYCGRG 1 cut(s) 1760
EcoO109I RGGNCCY 2 cut(s) 201, 368
EcoRI GAATTC 2 cut(s) 461, 1502
EcoRII CCWGG 7 cut(s) 97, 165, 195, 856, 958, 1776, 2200
EcoT14I CCWWGG 5 cut(s) 1297, 1917, 2061, 2096, 2163
EcoT38I GRGCYC 1 cut(s) 205
ErhI CCWWGG 5 cut(s) 1297, 1917, 2061, 2096, 2163
FaqI GGGAC 4 cut(s) 251, 629, 1591, 1777
FauNDI CATATG 1 cut(s) 803
FbaI TGATCA 1 cut(s) 1396
FblI GTMKAC 3 cut(s) 1333, 1497, 1685
Fnu4HI GCNGC 8 cut(s) 33, 57, 78, 81, 103, 631, 1584, 1659
FokI GGATG 4 cut(s) 376, 536, 1355, 2079
FriOI GRGCYC 1 cut(s) 205
Fsp4HI GCNGC 8 cut(s) 33, 57, 78, 81, 103, 631, 1584, 1659
FspBI CTAG 4 cut(s) 546, 1209, 1493, 2069
GlaI GCGC 2 cut(s) 529, 1602
GluI GCNGC 8 cut(s) 33, 57, 78, 81, 103, 631, 1584, 1659
GsaI CCCAGC 1 cut(s) 534
HaeII RGCGCY 2 cut(s) 531, 1604
HaeIII GGCC 7 cut(s) 14, 203, 657, 731, 817, 1997, 2041
HapII CCGG 1 cut(s) 209
HhaI GCGC 2 cut(s) 530, 1603
Hin6I GCGC 2 cut(s) 528, 1601
HinP1I GCGC 2 cut(s) 528, 1601
HincII GTYRAC 1 cut(s) 1408
HindII GTYRAC 1 cut(s) 1408
HinfI GANTC 2 cut(s) 1034, 1293
HpaI GTTAAC 1 cut(s) 1408
HpaII CCGG 1 cut(s) 209
HphI GGTGA 4 cut(s) 131, 1426, 1745, 2142
Hpy166II GTNNAC 7 cut(s) 560, 1324, 1334, 1408, 1498, 1686, 1828
Hpy188I TCNGA 6 cut(s) 109, 388, 449, 768, 1702, 2147
Hpy188III TCNNGA 7 cut(s) 361, 758, 1283, 1646, 1716, 1762, 2123
Hpy8I GTNNAC 7 cut(s) 560, 1324, 1334, 1408, 1498, 1686, 1828
Hpy99I CGWCG 1 cut(s) 1004
HpyAV CCTTC 6 cut(s) 649, 860, 1258, 1298, 1961, 2066
HpyCH4III ACNGT 5 cut(s) 191, 577, 939, 1321, 1753
HpyCH4IV ACGT 2 cut(s) 273, 1824
HpyF10VI GCNNNNNNNGC 6 cut(s) 89, 546, 644, 791, 1592, 2003
HpyF3I CTNAG 4 cut(s) 765, 1851, 1894, 2133
HpySE526I ACGT 2 cut(s) 273, 1824
HspAI GCGC 2 cut(s) 528, 1601
Ksp22I TGATCA 1 cut(s) 1396
KspAI GTTAAC 1 cut(s) 1408
Kzo9I GATC 7 cut(s) 6, 121, 1053, 1396, 1547, 2110, 2147
LmnI GCTCC 2 cut(s) 1709, 2065
Lsp1109I GCAGC 6 cut(s) 67, 89, 89, 642, 1595, 1670
LweI GCATC 5 cut(s) 192, 558, 1032, 1911, 2101
MaeI CTAG 4 cut(s) 546, 1209, 1493, 2069
MaeII ACGT 2 cut(s) 273, 1824
MaeIII GTNAC 6 cut(s) 161, 253, 314, 419, 980, 1820
MalI GATC 7 cut(s) 8, 123, 1055, 1398, 1549, 2112, 2149
MboI GATC 7 cut(s) 6, 121, 1053, 1396, 1547, 2110, 2147
MhlI GDGCHC 4 cut(s) 205, 1384, 1533, 1761
MlsI TGGCCA 1 cut(s) 1997
MluNI TGGCCA 1 cut(s) 1997
MlyI GAGTC 1 cut(s) 1302
Mox20I TGGCCA 1 cut(s) 1997
MroXI GAANNNNTTC 1 cut(s) 708
MscI TGGCCA 1 cut(s) 1997
MseI TTAA 6 cut(s) 621, 723, 1149, 1407, 1859, 1934
MslI CAYNNNNRTG 5 cut(s) 606, 885, 1443, 1647, 2240
Msp20I TGGCCA 1 cut(s) 1997
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 7 cut(s) 99, 167, 197, 858, 960, 1778, 2202
MvaI CCWGG 7 cut(s) 99, 167, 197, 858, 960, 1778, 2202
MwoI GCNNNNNNNGC 6 cut(s) 89, 546, 644, 791, 1592, 2003
NcoI CCATGG 3 cut(s) 1917, 2061, 2096
NdeI CATATG 1 cut(s) 803
NdeII GATC 7 cut(s) 6, 121, 1053, 1396, 1547, 2110, 2147
NlaIV GGNNCC 6 cut(s) 202, 203, 369, 1381, 1782, 2218
NmuCI GTSAC 2 cut(s) 419, 1820
NspI RCATGY 3 cut(s) 789, 2227, 2239
PaeI GCATGC 2 cut(s) 789, 2227
PagI TCATGA 2 cut(s) 757, 1282
PceI AGGCCT 2 cut(s) 817, 2041
PciI ACATGT 1 cut(s) 2235
PdmI GAANNNNTTC 1 cut(s) 708
PfeI GAWTC 1 cut(s) 1034
PfoI TCCNGGA 1 cut(s) 958
PkrI GCNGC 8 cut(s) 34, 58, 79, 82, 104, 632, 1585, 1660
PleI GAGTC 1 cut(s) 1301
PpsI GAGTC 1 cut(s) 1301
PpuMI RGGWCCY 1 cut(s) 368
PscI ACATGT 1 cut(s) 2235
PshAI GACNNNNGTC 1 cut(s) 753
Psp5II RGGWCCY 1 cut(s) 368
Psp6I CCWGG 7 cut(s) 97, 165, 195, 856, 958, 1776, 2200
PspFI CCCAGC 1 cut(s) 530
PspGI CCWGG 7 cut(s) 97, 165, 195, 856, 958, 1776, 2200
PspN4I GGNNCC 6 cut(s) 202, 203, 369, 1381, 1782, 2218
PspOMI GGGCCC 1 cut(s) 201
PspPI GGNCC 9 cut(s) 201, 202, 211, 368, 718, 729, 1780, 2198, 2217
PspPPI RGGWCCY 1 cut(s) 368
PstI CTGCAG 2 cut(s) 632, 2110
PstNI CAGNNNCTG 2 cut(s) 80, 83
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 4 cut(s) 574, 878, 1166, 2206
RsaNI GTAC 4 cut(s) 573, 877, 1165, 2205
RseI CAYNNNNRTG 5 cut(s) 606, 885, 1443, 1647, 2240
SaqAI TTAA 6 cut(s) 621, 723, 1149, 1407, 1859, 1934
SatI GCNGC 8 cut(s) 33, 57, 78, 81, 103, 631, 1584, 1659
Sau3AI GATC 7 cut(s) 6, 121, 1053, 1396, 1547, 2110, 2147
Sau96I GGNCC 9 cut(s) 201, 202, 211, 368, 718, 729, 1780, 2198, 2217
ScaI AGTACT 1 cut(s) 1166
SchI GAGTC 1 cut(s) 1302
ScrFI CCNGG 7 cut(s) 99, 167, 197, 858, 960, 1778, 2202
SduI GDGCHC 4 cut(s) 205, 1384, 1533, 1761
SfaNI GCATC 5 cut(s) 192, 558, 1032, 1911, 2101
SfcI CTRYAG 5 cut(s) 190, 628, 831, 1752, 2106
SinI GGWCC 6 cut(s) 211, 368, 718, 1780, 2198, 2217
SmiMI CAYNNNNRTG 5 cut(s) 606, 885, 1443, 1647, 2240
SmlI CTYRAG 1 cut(s) 1259
SmoI CTYRAG 1 cut(s) 1259
SpeI ACTAGT 1 cut(s) 1492
SphI GCATGC 2 cut(s) 789, 2227
SseBI AGGCCT 2 cut(s) 817, 2041
SsiI CCGC 6 cut(s) 32, 56, 117, 181, 1340, 1595
SspI AATATT 3 cut(s) 291, 1666, 1975
SspMI CTAG 4 cut(s) 546, 1209, 1493, 2069
StuI AGGCCT 2 cut(s) 817, 2041
StyD4I CCNGG 7 cut(s) 97, 165, 195, 856, 958, 1776, 2200
StyI CCWWGG 5 cut(s) 1297, 1917, 2061, 2096, 2163
TaaI ACNGT 5 cut(s) 191, 577, 939, 1321, 1753
TaiI ACGT 2 cut(s) 276, 1827
TaqI TCGA 4 cut(s) 5, 1223, 1370, 1645
TatI WGTACW 1 cut(s) 1164
TauI GCSGC 2 cut(s) 35, 59
TfiI GAWTC 1 cut(s) 1034
Tru1I TTAA 6 cut(s) 621, 723, 1149, 1407, 1859, 1934
Tru9I TTAA 6 cut(s) 621, 723, 1149, 1407, 1859, 1934
TscAI CASTG 5 cut(s) 508, 562, 706, 944, 2218
TseFI GTSAC 2 cut(s) 419, 1820
TseI GCWGC 6 cut(s) 77, 80, 102, 630, 1583, 1658
Tsp45I GTSAC 2 cut(s) 419, 1820
TspGWI ACGGA 1 cut(s) 948
TspRI CASTG 5 cut(s) 508, 562, 706, 944, 2218
VpaK11BI GGWCC 6 cut(s) 211, 368, 718, 1780, 2198, 2217
XapI RAATTY 3 cut(s) 461, 1502, 1889
XceI RCATGY 3 cut(s) 789, 2227, 2239
XmiI GTMKAC 3 cut(s) 1333, 1497, 1685
XmnI GAANNNNTTC 1 cut(s) 708
XspI CTAG 4 cut(s) 546, 1209, 1493, 2069
ZrmI AGTACT 1 cut(s) 1166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.