RchiOBHm_Chr5g0017401

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000066
Physical Location & Seq
Reverse (-)
12062038 .. 12065025
2988 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29770

Sequence Viewer

Length: 2148 bp
ATGGCCTTACATGGGAGGCCGCTTATTATGCAGCTGATCTCATTGGTAATGGTAGCTATAGAAGCAGCATCTGATCAAGCTTTGCCTCCTCAAGCCCTGCCTGGATGCCCCGGCCGGTGTGGTAATCTCACAATCCCGTATCCCTTCGGGATAGGGGATGGTTGTTATCTGCGACAAAGATTCAACATCACTTGTAACGAATCTGCCCATCCCCCAACAGCACTATTGGCGGATGGTAGTATCATTGCTACTAACATATCCCTGGATGAAGGTGAGTTGCAATTTTGGAATACCATAGCCCATGATTGTTATGATAAACAGGGCAATCAAACGGGGAGCACGAGCAATTCCCCTTCGCTCCAGTTGAATCCTCCTTTCACCATCTCTGGTACCAAAAACAACTTGATCGCTGTTGGCTGTGACACTTCTGCAATTTTCAAAGGAAACTTTCAATACCCTTATGATGAAGCGAGGTACATACTCACGTGCATGTCCATATGTTACAATCTTGACAGTGCTGTTAACGGCTCGTGCTCCTTCGGCTCCAGCATTGGGTGTTGCCAAACTGGACTCCCTAACGGACTAAAAAATTTTACTCTCACTTTGGGTAGCTTTTCTGAATATAAGGACGTCATATGGAACTTCAAGCCATGCAGCTACGCCTTCATCGTGGAAGAAGGCGAGTTCACATTCTTCCCGAATACAAGTTTTCAAGAACTGTCCAAGAATCAACAACTTCCCGTGATTCTTAATTGGGAAATTCAGGATGGGGGTTGTGCCGAAGCTCAAAAGAGGCATGATTATGCATGCAAGGCAAATAGCAGGTGTGTGAACCGGACGATCGACATCCGGACTGAACCGTCTGGTTACTATTGCCAGTGCTTGCCAGGCTTCGAAGGGAACCCGTATCTCCCAGACGGTTGCCGAGGTGTCAGTTTAGGCTTCTTAGTTTTGCTAGTTGTAATAATATGGATTCACTGGGAAATGAACAAAAGAAGGATTATCAAACTCAAAGAGAAGCACTTCAAAGAAAATGGCGGCTTAATGTTGCAGCAACAACTCGCTCGTCATGGAGGAAGGGTGGATACAACCAGAATATTTACTTCAGAAGAACTTGAGAAAGCCACAAACAATTACCATGAAAGTAGAATCCTTGGTGAAGGTGGCTATGGAACTGTTTATAAAGGAACATTACCGGATAACACAATGGTGGCCATAAAGAAGTCCAAAGTTGCTGCCCGAACTCAACATGATCAGTTTGTTAATGAGGTGATTTTGCTTTCTCAAATAAACCACAGAAATGTGGTGAGACTATTAGGTTGTTGTTTTGAGACAGAAGTGCCTTTACTAGTGTATGAGTACATCACCCATGGCACTCTTTTTGAGCATTTATTCAACAATAAAAACGGTAAAAGATCATCACTTTCGTGGGAATTACGATTGAAGATAGCATCGGAAACTGCAGGAGCACTAGCATACTTGCACTCTTCCACTTCCACACCAATCATACACCGGGATGTGAAAGCAACAAATATACTGTTGGATGAAAATTATACGGCCAAAGAGTCGGATTTTGGAGCTTCGCGTTTCATTCCTCTGGATCAAACTCAAGTAGCAACATTAGTGCAAGGCACAATGGGATACTTAGATCCCGAATATATGCAATCAAACACGCTAACAGAAAAGAGTGATGTTTATAGCTTTGGAGTTGTCCTAGCAGAGCTATTAACGAGCAAAGTGGCGGTTTCTTTTGCTAGGCCCGAGTCAGAAAGAAGCCTAGCATATTTATTTGTTTCCTCAATTGAAAAACAATGCCTGATTCAAATTCTTGATGCTGACATGGTGAATGAGGGAAATGTCGAGATGCTTGAGCAGGTGGCAAATTTGGCAAAAATATGTCTAAGGGTAAAAGGGAAGGAAAGGCCTACGATGAAGGAAGTGGCCATGGAGTTAGAGGGAATGAGAACTACGGCAAAGCATCCATGGAAAAATGCTGATTTCTATCCAGAGGAGACTGAGTACTTGTTGGGTTCTCCTAATTCCAAGTCTTACATTGAAGGTAAAGGTGAAGTTAGTTCTAGTGGTTCAGTCAGTAGGTATGCCAGCATGCAAAATGAAATGTTAAAGTCGTACGATGATGGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

715

Amino Acids

79.55

Weight (kDa)

5.77

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 36 - 93 6.1e-10 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 379 - 647 1.7e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 382 - 651 3e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1182
AarI CACCTGC 2 cut(s) 813, 1864
AasI GACNNNNNNGTC 1 cut(s) 859
AatII GACGTC 1 cut(s) 633
Acc36I ACCTGC 2 cut(s) 813, 1864
Acc65I GGTACC 1 cut(s) 389
AccB1I GGYRCC 1 cut(s) 389
AccII CGCG 1 cut(s) 1585
AccIII TCCGGA 1 cut(s) 849
AciI CCGC 4 cut(s) 20, 230, 1038, 1742
AclWI GGATC 2 cut(s) 1608, 1643
AcoI YGGCCR 4 cut(s) 112, 1212, 1557, 1941
AcsI RAATTY 4 cut(s) 589, 759, 1824, 1882
AcuI CTGAAG 1 cut(s) 1089
AcvI CACGTG 1 cut(s) 486
AcyI GRCGYC 1 cut(s) 630
AfaI GTAC 5 cut(s) 391, 476, 1361, 2021, 2132
AfiI CCNNNNNNNGG 2 cut(s) 12, 552
AhlI ACTAGT 1 cut(s) 1348
AjnI CCWGG 3 cut(s) 100, 261, 886
AleI CACNNNNGTG 2 cut(s) 1208, 1426
AluBI AGCT 9 cut(s) 34, 56, 80, 612, 657, 785, 1580, 1701, 1723
AluI AGCT 9 cut(s) 34, 56, 80, 612, 657, 785, 1580, 1701, 1723
Alw21I GWGCWC 3 cut(s) 341, 536, 1471
Alw26I GTCTC 3 cut(s) 1303, 1325, 2006
AlwI GGATC 2 cut(s) 1608, 1643
AlwNI CAGNNNCTG 1 cut(s) 71
Ama87I CYCGRG 1 cut(s) 1760
Aor13HI TCCGGA 1 cut(s) 849
AoxI GGCC 8 cut(s) 3, 17, 112, 1212, 1557, 1757, 1922, 1941
ApeKI GCWGC 5 cut(s) 31, 65, 654, 1051, 1235
ApoI RAATTY 4 cut(s) 589, 759, 1824, 1882
Asp700I GAANNNNTTC 1 cut(s) 1022
Asp718I GGTACC 1 cut(s) 389
AspS9I GGNCC 1 cut(s) 1758
AsuC2I CCSGG 2 cut(s) 111, 1514
AsuHPI GGTGA 8 cut(s) 284, 370, 1169, 1282, 1318, 1357, 1855, 2078
AsuII TTCGAA 1 cut(s) 894
AvaI CYCGRG 1 cut(s) 1760
BalI TGGCCA 2 cut(s) 1214, 1943
BanI GGYRCC 1 cut(s) 389
BarI GAAGNNNNNNTAC 4 cut(s) 1069, 1101, 1329, 1361
BauI CACGAG 2 cut(s) 340, 529
BbrPI CACGTG 1 cut(s) 486
Bbv12I GWGCWC 3 cut(s) 341, 536, 1471
BbvI GCAGC 5 cut(s) 43, 77, 666, 1063, 1222
BccI CCATC 6 cut(s) 152, 216, 227, 389, 761, 2132
BceAI ACGGC 3 cut(s) 541, 1572, 1986
BciT130I CCWGG 3 cut(s) 102, 263, 888
BciVI GTATCC 3 cut(s) 150, 1078, 1634
BclI TGATCA 2 cut(s) 73, 1252
BcnI CCSGG 2 cut(s) 111, 1514
BcoDI GTCTC 3 cut(s) 1303, 1325, 2006
BcuI ACTAGT 1 cut(s) 1348
BfaI CTAG 7 cut(s) 956, 1349, 1472, 1715, 1755, 1778, 2079
BfmI CTRYAG 2 cut(s) 57, 1461
BfuAI ACCTGC 2 cut(s) 813, 1864
BfuI GTATCC 3 cut(s) 150, 1078, 1634
BisI GCNGC 7 cut(s) 20, 32, 66, 655, 1039, 1052, 1236
BlsI GCNGC 7 cut(s) 21, 33, 67, 656, 1040, 1053, 1237
BmcAI AGTACT 1 cut(s) 2021
Bme1390I CCNGG 5 cut(s) 102, 111, 263, 888, 1514
BmeT110I CYCGRG 1 cut(s) 1760
BmgT120I GGNCC 1 cut(s) 1758
BmiI GGNNCC 3 cut(s) 391, 544, 902
BmrFI CCNGG 5 cut(s) 102, 111, 263, 888, 1514
BmrI ACTGGG 1 cut(s) 988
BmsI GCATC 6 cut(s) 77, 95, 1460, 1822, 1854, 1987
BmuI ACTGGG 1 cut(s) 988
BpmI CTGGAG 2 cut(s) 344, 529
Bpu14I TTCGAA 1 cut(s) 894
BpuEI CTTGAG 4 cut(s) 75, 1136, 1593, 1889
BpuMI CCSGG 2 cut(s) 111, 1514
BsaAI YACGTR 1 cut(s) 486
BsaBI GATNNNNATC 2 cut(s) 845, 2001
BsaHI GRCGYC 1 cut(s) 630
BsaJI CCNNGG 7 cut(s) 109, 261, 925, 1153, 1369, 1944, 1982
BsaWI WCCGGW 3 cut(s) 834, 849, 1195
BsaXI ACNNNNNCTCC 4 cut(s) 1065, 1095, 2003, 2033
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 552
Bse118I RCCGGY 1 cut(s) 114
Bse1I ACTGG 4 cut(s) 361, 571, 877, 983
Bse3DI GCAATG 1 cut(s) 243
Bse8I GATNNNNATC 2 cut(s) 845, 2001
BseAI TCCGGA 1 cut(s) 849
BseBI CCWGG 3 cut(s) 102, 263, 888
BseDI CCNNGG 7 cut(s) 109, 261, 925, 1153, 1369, 1944, 1982
BseJI GATNNNNATC 2 cut(s) 845, 2001
BseLI CCNNNNNNNGG 2 cut(s) 12, 552
BseMI GCAATG 1 cut(s) 243
BseMII CTCAG 1 cut(s) 2007
BseNI ACTGG 4 cut(s) 361, 571, 877, 983
BseRI GAGGAG 2 cut(s) 78, 2024
BseX3I CGGCCG 1 cut(s) 112
BseXI GCAGC 5 cut(s) 43, 77, 666, 1063, 1222
Bsh1236I CGCG 1 cut(s) 1585
Bsh1285I CGRYCG 2 cut(s) 115, 843
BshFI GGCC 8 cut(s) 5, 19, 114, 1214, 1559, 1759, 1924, 1943
BshNI GGYRCC 1 cut(s) 389
BsiEI CGRYCG 2 cut(s) 115, 843
BsiHKAI GWGCWC 3 cut(s) 341, 536, 1471
BsiHKCI CYCGRG 1 cut(s) 1760
BsiSI CCGG 6 cut(s) 111, 115, 835, 850, 1196, 1513
BsiWI CGTACG 1 cut(s) 2130
BslI CCNNNNNNNGG 2 cut(s) 12, 552
BsmAI GTCTC 3 cut(s) 1303, 1325, 2006
BsnI GGCC 8 cut(s) 5, 19, 114, 1214, 1559, 1759, 1924, 1943
BsoBI CYCGRG 1 cut(s) 1760
Bsp119I TTCGAA 1 cut(s) 894
Bsp1286I GDGCHC 3 cut(s) 341, 536, 1471
Bsp13I TCCGGA 1 cut(s) 849
Bsp143I GATC 8 cut(s) 36, 73, 405, 840, 1252, 1415, 1600, 1648
Bsp19I CCATGG 3 cut(s) 1369, 1944, 1982
BspACI CCGC 4 cut(s) 20, 230, 1038, 1742
BspANI GGCC 8 cut(s) 5, 19, 114, 1214, 1559, 1759, 1924, 1943
BspCNI CTCAG 1 cut(s) 2008
BspEI TCCGGA 1 cut(s) 849
BspFNI CGCG 1 cut(s) 1585
BspLI GGNNCC 3 cut(s) 391, 544, 902
BspMAI CTGCAG 1 cut(s) 1465
BspMI ACCTGC 2 cut(s) 813, 1864
BspPI GGATC 2 cut(s) 1608, 1643
BspT104I TTCGAA 1 cut(s) 894
BspT107I GGYRCC 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 243
BsrFI RCCGGY 1 cut(s) 114
BsrI ACTGG 4 cut(s) 361, 571, 877, 983
BssAI RCCGGY 1 cut(s) 114
BssECI CCNNGG 7 cut(s) 109, 261, 925, 1153, 1369, 1944, 1982
BssMI GATC 8 cut(s) 36, 73, 405, 840, 1252, 1415, 1600, 1648
BssNI GRCGYC 1 cut(s) 630
BssSI CACGAG 2 cut(s) 340, 529
BssT1I CCWWGG 4 cut(s) 1153, 1369, 1944, 1982
Bst2BI CACGAG 2 cut(s) 340, 529
Bst2UI CCWGG 3 cut(s) 102, 263, 888
Bst4CI ACNGT 7 cut(s) 515, 720, 861, 920, 1177, 1409, 1539
Bst6I CTCTTC 1 cut(s) 1492
BstACI GRCGYC 1 cut(s) 630
BstBAI YACGTR 1 cut(s) 486
BstBI TTCGAA 1 cut(s) 894
BstC8I GCNNGC 4 cut(s) 808, 884, 2104, 2108
BstDEI CTNAG 4 cut(s) 946, 1645, 1901, 2016
BstDSI CCRYGG 3 cut(s) 1369, 1944, 1982
BstFNI CGCG 1 cut(s) 1585
BstKTI GATC 8 cut(s) 39, 76, 408, 843, 1255, 1418, 1603, 1651
BstMAI GTCTC 3 cut(s) 1303, 1325, 2006
BstMBI GATC 8 cut(s) 36, 73, 405, 840, 1252, 1415, 1600, 1648
BstMCI CGRYCG 2 cut(s) 115, 843
BstMWI GCNNNNNNNGC 7 cut(s) 28, 62, 227, 540, 812, 888, 1886
BstNI CCWGG 3 cut(s) 102, 263, 888
BstNSI RCATGY 3 cut(s) 493, 810, 2110
BstSCI CCNGG 5 cut(s) 100, 109, 261, 886, 1512
BstSFI CTRYAG 2 cut(s) 57, 1461
BstUI CGCG 1 cut(s) 1585
BstV1I GCAGC 5 cut(s) 43, 77, 666, 1063, 1222
BstX2I RGATCY 1 cut(s) 1648
BstYI RGATCY 1 cut(s) 1648
BstZI CGGCCG 1 cut(s) 112
BsuI GTATCC 3 cut(s) 150, 1078, 1634
BsuRI GGCC 8 cut(s) 5, 19, 114, 1214, 1559, 1759, 1924, 1943
BtgI CCRYGG 3 cut(s) 1369, 1944, 1982
BtsIMutI CAGTG 3 cut(s) 520, 884, 976
BveI ACCTGC 2 cut(s) 813, 1864
Cac8I GCNNGC 4 cut(s) 808, 884, 2104, 2108
CaiI CAGNNNCTG 1 cut(s) 71
Cfr10I RCCGGY 1 cut(s) 114
Cfr13I GGNCC 1 cut(s) 1758
Csp6I GTAC 5 cut(s) 390, 475, 1360, 2020, 2131
CviQI GTAC 5 cut(s) 390, 475, 1360, 2020, 2131
DdeI CTNAG 4 cut(s) 946, 1645, 1901, 2016
DpnI GATC 8 cut(s) 38, 75, 407, 842, 1254, 1417, 1602, 1650
DpnII GATC 8 cut(s) 36, 73, 405, 840, 1252, 1415, 1600, 1648
DrdI GACNNNNNNGTC 1 cut(s) 859
DseDI GACNNNNNNGTC 1 cut(s) 859
EaeI YGGCCR 4 cut(s) 112, 1212, 1557, 1941
EagI CGGCCG 1 cut(s) 112
Eam1104I CTCTTC 1 cut(s) 1492
EarI CTCTTC 1 cut(s) 1492
EciI GGCGGA 1 cut(s) 245
EclXI CGGCCG 1 cut(s) 112
Eco130I CCWWGG 4 cut(s) 1153, 1369, 1944, 1982
Eco147I AGGCCT 1 cut(s) 1924
Eco52I CGGCCG 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 1089
Eco72I CACGTG 1 cut(s) 486
Eco88I CYCGRG 1 cut(s) 1760
EcoRII CCWGG 3 cut(s) 100, 261, 886
EcoT14I CCWWGG 4 cut(s) 1153, 1369, 1944, 1982
EcoT22I ATGCAT 1 cut(s) 808
ErhI CCWWGG 4 cut(s) 1153, 1369, 1944, 1982
FauNDI CATATG 2 cut(s) 497, 635
FbaI TGATCA 2 cut(s) 73, 1252
Fnu4HI GCNGC 7 cut(s) 20, 32, 66, 655, 1039, 1052, 1236
Fsp4HI GCNGC 7 cut(s) 20, 32, 66, 655, 1039, 1052, 1236
FspBI CTAG 7 cut(s) 956, 1349, 1472, 1715, 1755, 1778, 2079
GluI GCNGC 7 cut(s) 20, 32, 66, 655, 1039, 1052, 1236
GsuI CTGGAG 2 cut(s) 344, 529
HaeIII GGCC 8 cut(s) 5, 19, 114, 1214, 1559, 1759, 1924, 1943
HapII CCGG 6 cut(s) 111, 115, 835, 850, 1196, 1513
Hin1I GRCGYC 1 cut(s) 630
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HindIII AAGCTT 1 cut(s) 78
HpaI GTTAAC 1 cut(s) 523
HpaII CCGG 6 cut(s) 111, 115, 835, 850, 1196, 1513
HphI GGTGA 8 cut(s) 284, 370, 1169, 1282, 1318, 1357, 1855, 2078
Hpy166II GTNNAC 3 cut(s) 523, 687, 832
Hpy188I TCNGA 6 cut(s) 73, 619, 1108, 1456, 1570, 1768
Hpy8I GTNNAC 3 cut(s) 523, 687, 832
HpyCH4III ACNGT 7 cut(s) 515, 720, 861, 920, 1177, 1409, 1539
HpyCH4IV ACGT 2 cut(s) 485, 630
HpyF10VI GCNNNNNNNGC 7 cut(s) 28, 62, 227, 540, 812, 888, 1886
HpyF3I CTNAG 4 cut(s) 946, 1645, 1901, 2016
HpySE526I ACGT 2 cut(s) 485, 630
Hsp92I GRCGYC 1 cut(s) 630
Kpn2I TCCGGA 1 cut(s) 849
KpnI GGTACC 1 cut(s) 393
Ksp22I TGATCA 2 cut(s) 73, 1252
KspAI GTTAAC 1 cut(s) 523
Kzo9I GATC 8 cut(s) 36, 73, 405, 840, 1252, 1415, 1600, 1648
LmnI GCTCC 6 cut(s) 336, 363, 539, 548, 1466, 1577
Lsp1109I GCAGC 5 cut(s) 43, 77, 666, 1063, 1222
LweI GCATC 6 cut(s) 77, 95, 1460, 1822, 1854, 1987
MaeI CTAG 7 cut(s) 956, 1349, 1472, 1715, 1755, 1778, 2079
MaeII ACGT 2 cut(s) 485, 630
MaeIII GTNAC 4 cut(s) 194, 419, 500, 866
MalI GATC 8 cut(s) 38, 75, 407, 842, 1254, 1417, 1602, 1650
MboI GATC 8 cut(s) 36, 73, 405, 840, 1252, 1415, 1600, 1648
MboII GAAGA 5 cut(s) 685, 686, 1121, 1456, 1479
MfeI CAATTG 1 cut(s) 1800
MflI RGATCY 1 cut(s) 1648
MhlI GDGCHC 3 cut(s) 341, 536, 1471
MlsI TGGCCA 2 cut(s) 1214, 1943
MluNI TGGCCA 2 cut(s) 1214, 1943
MlyI GAGTC 3 cut(s) 564, 1574, 1772
MmeI TCCRAC 2 cut(s) 1521, 1548
Mox20I TGGCCA 2 cut(s) 1214, 1943
Mph1103I ATGCAT 1 cut(s) 808
MroI TCCGGA 1 cut(s) 849
MroXI GAANNNNTTC 1 cut(s) 1022
MscI TGGCCA 2 cut(s) 1214, 1943
MseI TTAA 6 cut(s) 522, 750, 1043, 1263, 1727, 2123
MslI CAYNNNNRTG 6 cut(s) 488, 801, 1208, 1299, 1426, 1515
Msp20I TGGCCA 2 cut(s) 1214, 1943
MspA1I CMGCKG 1 cut(s) 34
MspI CCGG 6 cut(s) 111, 115, 835, 850, 1196, 1513
MspR9I CCNGG 5 cut(s) 102, 111, 263, 888, 1514
MunI CAATTG 1 cut(s) 1800
MvaI CCWGG 3 cut(s) 102, 263, 888
MvnI CGCG 1 cut(s) 1585
MwoI GCNNNNNNNGC 7 cut(s) 28, 62, 227, 540, 812, 888, 1886
NciI CCSGG 2 cut(s) 111, 1514
NcoI CCATGG 3 cut(s) 1369, 1944, 1982
NdeI CATATG 2 cut(s) 497, 635
NdeII GATC 8 cut(s) 36, 73, 405, 840, 1252, 1415, 1600, 1648
NlaIV GGNNCC 3 cut(s) 391, 544, 902
NmeAIII GCCGAG 1 cut(s) 950
NmuCI GTSAC 1 cut(s) 419
NsiI ATGCAT 1 cut(s) 808
NspI RCATGY 3 cut(s) 493, 810, 2110
NspV TTCGAA 1 cut(s) 894
OliI CACNNNNGTG 2 cut(s) 1208, 1426
PaeI GCATGC 2 cut(s) 810, 2110
PaqCI CACCTGC 2 cut(s) 813, 1864
PceI AGGCCT 1 cut(s) 1924
PcsI WCGNNNNNNNCGW 2 cut(s) 338, 666
PdmI GAANNNNTTC 1 cut(s) 1022
PfeI GAWTC 8 cut(s) 180, 200, 367, 727, 745, 973, 1149, 1819
Pfl23II CGTACG 1 cut(s) 2130
PkrI GCNGC 7 cut(s) 21, 33, 67, 656, 1040, 1053, 1237
Ple19I CGATCG 1 cut(s) 843
PleI GAGTC 3 cut(s) 564, 1573, 1771
PmaCI CACGTG 1 cut(s) 486
PmlI CACGTG 1 cut(s) 486
PpsI GAGTC 3 cut(s) 564, 1573, 1771
Ppu21I YACGTR 1 cut(s) 486
PsiI TTATAA 1 cut(s) 1182
Psp6I CCWGG 3 cut(s) 100, 261, 886
PspCI CACGTG 1 cut(s) 486
PspGI CCWGG 3 cut(s) 100, 261, 886
PspLI CGTACG 1 cut(s) 2130
PspN4I GGNNCC 3 cut(s) 391, 544, 902
PspPI GGNCC 1 cut(s) 1758
PstI CTGCAG 1 cut(s) 1465
PstNI CAGNNNCTG 1 cut(s) 71
PsuI RGATCY 1 cut(s) 1648
PvuI CGATCG 1 cut(s) 843
PvuII CAGCTG 1 cut(s) 34
RsaI GTAC 5 cut(s) 391, 476, 1361, 2021, 2132
RsaNI GTAC 5 cut(s) 390, 475, 1360, 2020, 2131
RseI CAYNNNNRTG 6 cut(s) 488, 801, 1208, 1299, 1426, 1515
SaqAI TTAA 6 cut(s) 522, 750, 1043, 1263, 1727, 2123
SatI GCNGC 7 cut(s) 20, 32, 66, 655, 1039, 1052, 1236
Sau3AI GATC 8 cut(s) 36, 73, 405, 840, 1252, 1415, 1600, 1648
Sau96I GGNCC 1 cut(s) 1758
ScaI AGTACT 1 cut(s) 2021
SchI GAGTC 3 cut(s) 564, 1574, 1772
ScrFI CCNGG 5 cut(s) 102, 111, 263, 888, 1514
SduI GDGCHC 3 cut(s) 341, 536, 1471
SfaNI GCATC 6 cut(s) 77, 95, 1460, 1822, 1854, 1987
SfcI CTRYAG 2 cut(s) 57, 1461
SfuI TTCGAA 1 cut(s) 894
SmiMI CAYNNNNRTG 6 cut(s) 488, 801, 1208, 1299, 1426, 1515
SmlI CTYRAG 4 cut(s) 90, 1115, 1608, 1868
SmoI CTYRAG 4 cut(s) 90, 1115, 1608, 1868
SpeI ACTAGT 1 cut(s) 1348
SphI GCATGC 2 cut(s) 810, 2110
SseBI AGGCCT 1 cut(s) 1924
SsiI CCGC 4 cut(s) 20, 230, 1038, 1742
SspI AATATT 1 cut(s) 1098
SspMI CTAG 7 cut(s) 956, 1349, 1472, 1715, 1755, 1778, 2079
StuI AGGCCT 1 cut(s) 1924
StyD4I CCNGG 5 cut(s) 100, 109, 261, 886, 1512
StyI CCWWGG 4 cut(s) 1153, 1369, 1944, 1982
TaaI ACNGT 7 cut(s) 515, 720, 861, 920, 1177, 1409, 1539
TaiI ACGT 2 cut(s) 488, 633
TaqI TCGA 3 cut(s) 843, 894, 1860
TatI WGTACW 2 cut(s) 1359, 2019
TauI GCSGC 2 cut(s) 22, 1041
TfiI GAWTC 8 cut(s) 180, 200, 367, 727, 745, 973, 1149, 1819
Tru1I TTAA 6 cut(s) 522, 750, 1043, 1263, 1727, 2123
Tru9I TTAA 6 cut(s) 522, 750, 1043, 1263, 1727, 2123
TscAI CASTG 3 cut(s) 520, 884, 983
TseFI GTSAC 1 cut(s) 419
TseI GCWGC 5 cut(s) 31, 65, 654, 1051, 1235
Tsp45I GTSAC 1 cut(s) 419
TspDTI ATGAA 9 cut(s) 282, 480, 655, 1001, 1155, 1560, 1579, 1946, 2130
TspGWI ACGGA 1 cut(s) 594
TspRI CASTG 3 cut(s) 520, 884, 983
XapI RAATTY 4 cut(s) 589, 759, 1824, 1882
XceI RCATGY 3 cut(s) 493, 810, 2110
XmnI GAANNNNTTC 1 cut(s) 1022
XspI CTAG 7 cut(s) 956, 1349, 1472, 1715, 1755, 1778, 2079
ZraI GACGTC 1 cut(s) 631
ZrmI AGTACT 1 cut(s) 2021
Zsp2I ATGCAT 1 cut(s) 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.