Wall-associated receptor kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 561 |
| Acc36I | ACCTGC | 1 cut(s) | 168 |
| AccI | GTMKAC | 3 cut(s) | 1323, 1333, 1497 |
| AciI | CCGC | 3 cut(s) | 47, 111, 1250 |
| AclWI | GGATC | 1 cut(s) | 2155 |
| AcoI | YGGCCR | 1 cut(s) | 1995 |
| AcsI | RAATTY | 4 cut(s) | 170, 455, 940, 1889 |
| AcuI | CTGAAG | 4 cut(s) | 1153, 1244, 1608, 1713 |
| AcyI | GRCGYC | 1 cut(s) | 522 |
| AfaI | GTAC | 4 cut(s) | 55, 568, 1166, 2206 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 818 |
| AflIII | ACRYGT | 2 cut(s) | 279, 2235 |
| AgsI | TTSAA | 5 cut(s) | 658, 688, 912, 1029, 1565 |
| AjnI | CCWGG | 4 cut(s) | 847, 949, 1776, 2200 |
| AleI | CACNNNNGTG | 1 cut(s) | 747 |
| Alw21I | GWGCWC | 4 cut(s) | 938, 1603, 1761, 2139 |
| Alw26I | GTCTC | 3 cut(s) | 337, 731, 2123 |
| AlwI | GGATC | 1 cut(s) | 2155 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 77 |
| Ama87I | CYCGRG | 1 cut(s) | 1760 |
| AoxI | GGCC | 3 cut(s) | 649, 1995, 2039 |
| ApoI | RAATTY | 4 cut(s) | 170, 455, 940, 1889 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 253, 702, 1047, 1257 |
| AspS9I | GGNCC | 2 cut(s) | 1780, 2198 |
| AsuHPI | GGTGA | 4 cut(s) | 936, 1745, 1996, 2142 |
| AvaI | CYCGRG | 1 cut(s) | 1760 |
| AvaII | GGWCC | 2 cut(s) | 1780, 2198 |
| BalI | TGGCCA | 1 cut(s) | 1997 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 1473, 1505, 1728, 1760 |
| BbsI | GAAGAC | 1 cut(s) | 2238 |
| Bbv12I | GWGCWC | 4 cut(s) | 938, 1603, 1761, 2139 |
| BccI | CCATC | 7 cut(s) | 879, 986, 1558, 1670, 1975, 2180, 2252 |
| BceAI | ACGGC | 1 cut(s) | 389 |
| BciT130I | CCWGG | 4 cut(s) | 849, 951, 1778, 2202 |
| BciVI | GTATCC | 3 cut(s) | 974, 1766, 2103 |
| BcoDI | GTCTC | 3 cut(s) | 337, 731, 2123 |
| BfaI | CTAG | 5 cut(s) | 51, 260, 1604, 2069, 2220 |
| BfmI | CTRYAG | 3 cut(s) | 622, 1752, 2106 |
| BfuAI | ACCTGC | 1 cut(s) | 168 |
| BfuI | GTATCC | 3 cut(s) | 974, 1766, 2103 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 927 |
| BlpI | GCTNAGC | 1 cut(s) | 1851 |
| BmcAI | AGTACT | 2 cut(s) | 55, 1166 |
| Bme1390I | CCNGG | 4 cut(s) | 849, 951, 1778, 2202 |
| Bme18I | GGWCC | 2 cut(s) | 1780, 2198 |
| BmeT110I | CYCGRG | 1 cut(s) | 1760 |
| BmgT120I | GGNCC | 2 cut(s) | 1780, 2198 |
| BmiI | GGNNCC | 2 cut(s) | 1222, 1782 |
| BmrFI | CCNGG | 4 cut(s) | 849, 951, 1778, 2202 |
| BmrI | ACTGGG | 1 cut(s) | 543 |
| BmsI | GCATC | 2 cut(s) | 1023, 1911 |
| BmuI | ACTGGG | 1 cut(s) | 543 |
| BpiI | GAAGAC | 1 cut(s) | 2238 |
| BpmI | CTGGAG | 2 cut(s) | 288, 933 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1851 |
| BpuEI | CTTGAG | 1 cut(s) | 66 |
| BsaHI | GRCGYC | 1 cut(s) | 522 |
| BsaI | GGTCTC | 1 cut(s) | 337 |
| BsaJI | CCNNGG | 9 cut(s) | 347, 433, 442, 1224, 1513, 1917, 2061, 2096, 2163 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 58, 88, 1209, 1239 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 818 |
| Bse1I | ACTGG | 8 cut(s) | 469, 549, 652, 714, 838, 875, 1209, 2211 |
| Bse3DI | GCAATG | 1 cut(s) | 636 |
| BseBI | CCWGG | 4 cut(s) | 849, 951, 1778, 2202 |
| BseDI | CCNNGG | 9 cut(s) | 347, 433, 442, 1224, 1513, 1917, 2061, 2096, 2163 |
| BseGI | GGATG | 3 cut(s) | 429, 543, 871 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 818 |
| BseMI | GCAATG | 1 cut(s) | 636 |
| BseMII | CTCAG | 3 cut(s) | 114, 1053, 1842 |
| BseNI | ACTGG | 8 cut(s) | 469, 549, 652, 714, 838, 875, 1209, 2211 |
| BseRI | GAGGAG | 1 cut(s) | 1609 |
| BsgI | GTGCAG | 3 cut(s) | 542, 1101, 1778 |
| BshFI | GGCC | 3 cut(s) | 651, 1997, 2041 |
| BsiHKAI | GWGCWC | 4 cut(s) | 938, 1603, 1761, 2139 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1760 |
| BslFI | GGGAC | 2 cut(s) | 623, 1777 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 818 |
| BsmAI | GTCTC | 3 cut(s) | 337, 731, 2123 |
| BsmFI | GGGAC | 2 cut(s) | 623, 1777 |
| BsmI | GAATGC | 1 cut(s) | 595 |
| BsnI | GGCC | 3 cut(s) | 651, 1997, 2041 |
| Bso31I | GGTCTC | 1 cut(s) | 337 |
| BsoBI | CYCGRG | 1 cut(s) | 1760 |
| Bsp1286I | GDGCHC | 4 cut(s) | 938, 1603, 1761, 2139 |
| Bsp143I | GATC | 2 cut(s) | 2110, 2147 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1851 |
| Bsp19I | CCATGG | 3 cut(s) | 1513, 1917, 2096 |
| BspACI | CCGC | 3 cut(s) | 47, 111, 1250 |
| BspANI | GGCC | 3 cut(s) | 651, 1997, 2041 |
| BspCNI | CTCAG | 3 cut(s) | 113, 1052, 1843 |
| BspHI | TCATGA | 2 cut(s) | 1282, 1525 |
| BspLI | GGNNCC | 2 cut(s) | 1222, 1782 |
| BspMAI | CTGCAG | 2 cut(s) | 626, 2110 |
| BspMI | ACCTGC | 1 cut(s) | 168 |
| BspPI | GGATC | 1 cut(s) | 2155 |
| BspTNI | GGTCTC | 1 cut(s) | 337 |
| BsrDI | GCAATG | 1 cut(s) | 636 |
| BsrI | ACTGG | 8 cut(s) | 469, 549, 652, 714, 838, 875, 1209, 2211 |
| BssECI | CCNNGG | 9 cut(s) | 347, 433, 442, 1224, 1513, 1917, 2061, 2096, 2163 |
| BssMI | GATC | 2 cut(s) | 2110, 2147 |
| BssNAI | GTATAC | 3 cut(s) | 1324, 1334, 1498 |
| BssNI | GRCGYC | 1 cut(s) | 522 |
| BssT1I | CCWWGG | 5 cut(s) | 1513, 1917, 2061, 2096, 2163 |
| Bst1107I | GTATAC | 3 cut(s) | 1324, 1334, 1498 |
| Bst2UI | CCWGG | 4 cut(s) | 849, 951, 1778, 2202 |
| Bst4CI | ACNGT | 3 cut(s) | 563, 571, 1753 |
| Bst6I | CTCTTC | 2 cut(s) | 1527, 2121 |
| BstACI | GRCGYC | 1 cut(s) | 522 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 77 |
| BstC8I | GCNNGC | 5 cut(s) | 143, 845, 1084, 1900, 2225 |
| BstDEI | CTNAG | 4 cut(s) | 100, 1039, 1851, 1894 |
| BstDSI | CCRYGG | 4 cut(s) | 1224, 1513, 1917, 2096 |
| BstF5I | GGATG | 3 cut(s) | 429, 543, 871 |
| BstKTI | GATC | 2 cut(s) | 2113, 2150 |
| BstMAI | GTCTC | 3 cut(s) | 337, 731, 2123 |
| BstMBI | GATC | 2 cut(s) | 2110, 2147 |
| BstNI | CCWGG | 4 cut(s) | 849, 951, 1778, 2202 |
| BstNSI | RCATGY | 5 cut(s) | 145, 283, 475, 2227, 2239 |
| BstSCI | CCNGG | 4 cut(s) | 847, 949, 1776, 2200 |
| BstSFI | CTRYAG | 3 cut(s) | 622, 1752, 2106 |
| BstV2I | GAAGAC | 1 cut(s) | 2238 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 878 |
| BstZ17I | GTATAC | 3 cut(s) | 1324, 1334, 1498 |
| BsuI | GTATCC | 3 cut(s) | 974, 1766, 2103 |
| BsuRI | GGCC | 3 cut(s) | 651, 1997, 2041 |
| BtgI | CCRYGG | 4 cut(s) | 1224, 1513, 1917, 2096 |
| BtsCI | GGATG | 3 cut(s) | 429, 543, 871 |
| BtsIMutI | CAGTG | 6 cut(s) | 556, 693, 707, 845, 1252, 2218 |
| BveI | ACCTGC | 1 cut(s) | 168 |
| Cac8I | GCNNGC | 5 cut(s) | 143, 845, 1084, 1900, 2225 |
| CaiI | CAGNNNCTG | 1 cut(s) | 77 |
| CciI | TCATGA | 2 cut(s) | 1282, 1525 |
| Cfr13I | GGNCC | 2 cut(s) | 1780, 2198 |
| CseI | GACGC | 3 cut(s) | 511, 790, 1238 |
| Csp6I | GTAC | 4 cut(s) | 54, 567, 1165, 2205 |
| CspCI | CAANNNNNGTGG | 3 cut(s) | 35, 1851, 1886 |
| CviQI | GTAC | 4 cut(s) | 54, 567, 1165, 2205 |
| DdeI | CTNAG | 4 cut(s) | 100, 1039, 1851, 1894 |
| DpnI | GATC | 2 cut(s) | 2112, 2149 |
| DpnII | GATC | 2 cut(s) | 2110, 2147 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 561 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 561 |
| EaeI | YGGCCR | 1 cut(s) | 1995 |
| Eam1104I | CTCTTC | 2 cut(s) | 1527, 2121 |
| EarI | CTCTTC | 2 cut(s) | 1527, 2121 |
| Eco130I | CCWWGG | 5 cut(s) | 1513, 1917, 2061, 2096, 2163 |
| Eco147I | AGGCCT | 1 cut(s) | 2041 |
| Eco31I | GGTCTC | 1 cut(s) | 337 |
| Eco32I | GATATC | 1 cut(s) | 775 |
| Eco47I | GGWCC | 2 cut(s) | 1780, 2198 |
| Eco57I | CTGAAG | 4 cut(s) | 1153, 1244, 1608, 1713 |
| Eco88I | CYCGRG | 1 cut(s) | 1760 |
| EcoRI | GAATTC | 2 cut(s) | 170, 455 |
| EcoRII | CCWGG | 4 cut(s) | 847, 949, 1776, 2200 |
| EcoRV | GATATC | 1 cut(s) | 775 |
| EcoT14I | CCWWGG | 5 cut(s) | 1513, 1917, 2061, 2096, 2163 |
| EcoT22I | ATGCAT | 1 cut(s) | 147 |
| ErhI | CCWWGG | 5 cut(s) | 1513, 1917, 2061, 2096, 2163 |
| FaqI | GGGAC | 2 cut(s) | 623, 1777 |
| FauNDI | CATATG | 2 cut(s) | 601, 2251 |
| FblI | GTMKAC | 3 cut(s) | 1323, 1333, 1497 |
| FokI | GGATG | 3 cut(s) | 416, 530, 858 |
| FspBI | CTAG | 5 cut(s) | 51, 260, 1604, 2069, 2220 |
| GsuI | CTGGAG | 2 cut(s) | 288, 933 |
| HaeIII | GGCC | 3 cut(s) | 651, 1997, 2041 |
| HgaI | GACGC | 3 cut(s) | 511, 790, 1238 |
| Hin1I | GRCGYC | 1 cut(s) | 522 |
| HincII | GTYRAC | 1 cut(s) | 1408 |
| HindII | GTYRAC | 1 cut(s) | 1408 |
| HindIII | AAGCTT | 1 cut(s) | 1454 |
| HinfI | GANTC | 4 cut(s) | 703, 1025, 1035, 1534 |
| HphI | GGTGA | 4 cut(s) | 936, 1745, 1996, 2142 |
| Hpy166II | GTNNAC | 7 cut(s) | 554, 1228, 1324, 1334, 1408, 1498, 1828 |
| Hpy188III | TCNNGA | 7 cut(s) | 260, 1283, 1526, 1646, 1716, 1762, 2123 |
| Hpy8I | GTNNAC | 7 cut(s) | 554, 1228, 1324, 1334, 1408, 1498, 1828 |
| Hpy99I | CGWCG | 1 cut(s) | 527 |
| HpyAV | CCTTC | 6 cut(s) | 643, 763, 851, 1258, 1961, 2066 |
| HpyCH4III | ACNGT | 3 cut(s) | 563, 571, 1753 |
| HpyF3I | CTNAG | 4 cut(s) | 100, 1039, 1851, 1894 |
| Hsp92I | GRCGYC | 1 cut(s) | 522 |
| Kzo9I | GATC | 2 cut(s) | 2110, 2147 |
| LmnI | GCTCC | 4 cut(s) | 79, 1598, 1709, 2065 |
| LweI | GCATC | 2 cut(s) | 1023, 1911 |
| MaeI | CTAG | 5 cut(s) | 51, 260, 1604, 2069, 2220 |
| MaeIII | GTNAC | 9 cut(s) | 155, 305, 413, 474, 827, 851, 971, 1063, 1191 |
| MalI | GATC | 2 cut(s) | 2112, 2149 |
| MboI | GATC | 2 cut(s) | 2110, 2147 |
| MfeI | CAATTG | 1 cut(s) | 726 |
| MhlI | GDGCHC | 4 cut(s) | 938, 1603, 1761, 2139 |
| MlsI | TGGCCA | 1 cut(s) | 1997 |
| MluNI | TGGCCA | 1 cut(s) | 1997 |
| MlyI | GAGTC | 1 cut(s) | 1029 |
| Mox20I | TGGCCA | 1 cut(s) | 1997 |
| Mph1103I | ATGCAT | 1 cut(s) | 147 |
| MroXI | GAANNNNTTC | 4 cut(s) | 253, 702, 1047, 1257 |
| MscI | TGGCCA | 1 cut(s) | 1997 |
| MseI | TTAA | 4 cut(s) | 218, 615, 1859, 1934 |
| MslI | CAYNNNNRTG | 7 cut(s) | 600, 604, 747, 1117, 1443, 1647, 2240 |
| Msp20I | TGGCCA | 1 cut(s) | 1997 |
| MspA1I | CMGCKG | 2 cut(s) | 798, 1586 |
| MspR9I | CCNGG | 4 cut(s) | 849, 951, 1778, 2202 |
| MunI | CAATTG | 1 cut(s) | 726 |
| Mva1269I | GAATGC | 1 cut(s) | 595 |
| MvaI | CCWGG | 4 cut(s) | 849, 951, 1778, 2202 |
| NcoI | CCATGG | 3 cut(s) | 1513, 1917, 2096 |
| NdeI | CATATG | 2 cut(s) | 601, 2251 |
| NdeII | GATC | 2 cut(s) | 2110, 2147 |
| NlaIV | GGNNCC | 2 cut(s) | 1222, 1782 |
| NmeAIII | GCCGAG | 1 cut(s) | 328 |
| NmuCI | GTSAC | 3 cut(s) | 413, 474, 971 |
| NsiI | ATGCAT | 1 cut(s) | 147 |
| NspI | RCATGY | 5 cut(s) | 145, 283, 475, 2227, 2239 |
| OliI | CACNNNNGTG | 1 cut(s) | 747 |
| PaeI | GCATGC | 2 cut(s) | 145, 2227 |
| PagI | TCATGA | 2 cut(s) | 1282, 1525 |
| PceI | AGGCCT | 1 cut(s) | 2041 |
| PciI | ACATGT | 2 cut(s) | 279, 2235 |
| PctI | GAATGC | 1 cut(s) | 595 |
| PdmI | GAANNNNTTC | 4 cut(s) | 253, 702, 1047, 1257 |
| PfeI | GAWTC | 3 cut(s) | 703, 1025, 1534 |
| PfoI | TCCNGGA | 1 cut(s) | 949 |
| PleI | GAGTC | 1 cut(s) | 1029 |
| PpsI | GAGTC | 1 cut(s) | 1029 |
| PscI | ACATGT | 2 cut(s) | 279, 2235 |
| Psp6I | CCWGG | 4 cut(s) | 847, 949, 1776, 2200 |
| PspGI | CCWGG | 4 cut(s) | 847, 949, 1776, 2200 |
| PspN4I | GGNNCC | 2 cut(s) | 1222, 1782 |
| PspPI | GGNCC | 2 cut(s) | 1780, 2198 |
| PstI | CTGCAG | 2 cut(s) | 626, 2110 |
| PstNI | CAGNNNCTG | 1 cut(s) | 77 |
| PvuII | CAGCTG | 2 cut(s) | 798, 1586 |
| RsaI | GTAC | 4 cut(s) | 55, 568, 1166, 2206 |
| RsaNI | GTAC | 4 cut(s) | 54, 567, 1165, 2205 |
| RseI | CAYNNNNRTG | 7 cut(s) | 600, 604, 747, 1117, 1443, 1647, 2240 |
| SaqAI | TTAA | 4 cut(s) | 218, 615, 1859, 1934 |
| Sau3AI | GATC | 2 cut(s) | 2110, 2147 |
| Sau96I | GGNCC | 2 cut(s) | 1780, 2198 |
| ScaI | AGTACT | 2 cut(s) | 55, 1166 |
| SchI | GAGTC | 1 cut(s) | 1029 |
| ScrFI | CCNGG | 4 cut(s) | 849, 951, 1778, 2202 |
| SduI | GDGCHC | 4 cut(s) | 938, 1603, 1761, 2139 |
| SfaNI | GCATC | 2 cut(s) | 1023, 1911 |
| SfcI | CTRYAG | 3 cut(s) | 622, 1752, 2106 |
| SinI | GGWCC | 2 cut(s) | 1780, 2198 |
| SmiMI | CAYNNNNRTG | 7 cut(s) | 600, 604, 747, 1117, 1443, 1647, 2240 |
| SmlI | CTYRAG | 1 cut(s) | 81 |
| SmoI | CTYRAG | 1 cut(s) | 81 |
| SphI | GCATGC | 2 cut(s) | 145, 2227 |
| SseBI | AGGCCT | 1 cut(s) | 2041 |
| SsiI | CCGC | 3 cut(s) | 47, 111, 1250 |
| SspI | AATATT | 1 cut(s) | 1975 |
| SspMI | CTAG | 5 cut(s) | 51, 260, 1604, 2069, 2220 |
| StuI | AGGCCT | 1 cut(s) | 2041 |
| StyD4I | CCNGG | 4 cut(s) | 847, 949, 1776, 2200 |
| StyI | CCWWGG | 5 cut(s) | 1513, 1917, 2061, 2096, 2163 |
| TaaI | ACNGT | 3 cut(s) | 563, 571, 1753 |
| TaqI | TCGA | 2 cut(s) | 325, 1645 |
| TatI | WGTACW | 2 cut(s) | 53, 1164 |
| TauI | GCSGC | 2 cut(s) | 50, 114 |
| TfiI | GAWTC | 3 cut(s) | 703, 1025, 1534 |
| Tru1I | TTAA | 4 cut(s) | 218, 615, 1859, 1934 |
| Tru9I | TTAA | 4 cut(s) | 218, 615, 1859, 1934 |
| TscAI | CASTG | 6 cut(s) | 556, 700, 714, 845, 1252, 2218 |
| TseFI | GTSAC | 3 cut(s) | 413, 474, 971 |
| Tsp45I | GTSAC | 3 cut(s) | 413, 474, 971 |
| TspGWI | ACGGA | 1 cut(s) | 1213 |
| TspRI | CASTG | 6 cut(s) | 556, 700, 714, 845, 1252, 2218 |
| VpaK11BI | GGWCC | 2 cut(s) | 1780, 2198 |
| XapI | RAATTY | 4 cut(s) | 170, 455, 940, 1889 |
| XbaI | TCTAGA | 1 cut(s) | 259 |
| XceI | RCATGY | 5 cut(s) | 145, 283, 475, 2227, 2239 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 845 |
| XmiI | GTMKAC | 3 cut(s) | 1323, 1333, 1497 |
| XmnI | GAANNNNTTC | 4 cut(s) | 253, 702, 1047, 1257 |
| XspI | CTAG | 5 cut(s) | 51, 260, 1604, 2069, 2220 |
| ZrmI | AGTACT | 2 cut(s) | 55, 1166 |
| Zsp2I | ATGCAT | 1 cut(s) | 147 |