Rw5G011080

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000066
Physical Location & Seq
Reverse (-)
12309850 .. 12312782
2933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G011080.1

Sequence Viewer

Length: 2283 bp
ATGTCGATCATGGCCTTGCAATTCCCATTGGCGGCAGTCCTAATCCTATTATCGGCGGCTAGTACTACGACAACAGCAGCTGCTGCTCAAACCCTCCCAGCTGACTGCCCAGACAAGTGCGGCAATCTCACAATTCCATATCCTTTTGGCATGAAGAAGGGTTGTTACCTGCGAGAAGCATTCTTTATCAACTGCAACTATACGACCCAACCACCAACAGCATATTTGATGACTGGGAATCTCGTTGTTACTAACATTTCCCTTCATGAAGGTGAGTTGCGAATATTAAACTGGTTAGCTATTGATTGTTACGATGCATATGGTCCAACTAACAGATCCAGCCCTCTGCTCACCCTGCCTCTTCCTTATACGATCTCTGATACTAAAAACATATTCATTGCCGTTGGTTGCGACACTTCCGCAGCTTTCCAAGGCTACCGAGGGGCAAAAGGTCGGTCGAGGTCCTTTAGTGGGTGCATGTCAGTATGCGACAGCCTTGGCAGTGTTGATAACAACTCTTGCTCTGGCGTTGGGTGTTGCGAGACTAAGATCCCCAGTGGACTGCACAATGTTAGTCTGAGTTTGGGTAGCTACTTCAATCATTCGTATGTGTGGGAATTTAACCCCTGCAGCTACGCCTTCATTGCACTGAAAGACCAATTCAATTTCACCTCTTCTAGTCTGAATGGCATTAAACCGCTTCCAATGGTTCTCAATTGGGCCATTGGGAATGACACAGACCCCTGTGATGAAGCTCAACACCGGACGGATTTCGCATGCATGGCTAATAGCAATTGCATAAACCGTCATATTAATGGGTCTGTTGGTTACTTGTGCCAGTGCTTGCCTGGTTATGAAGGAAACCCGTACCACCCAGATGGTTGCCGAGATATTGATGAGTGCAGGCCTTTCAACCCCTGCCAGAACGGACAGTGCTTAAATATTCCAGGAAATCACTCTTGTTCATGTCACAAAGGATACAAAAACGACGGCCTGAATGACAAGACTTGCATCAAAGACGATTCCGAAAGACACTCAAAGATCGTTCTCCTGCTTCTGGTTTCATTGGGTGTAACTGTGGGCATCTTGGTTTTATTCCTTGGAATTTCGTGGACATGTTGGGGAATGAAGAAAAGAGAGTTCATTAAACTCAAAGAAAAGCACTTCAAAGAGAATGGTGGCTTATTGTTACTGCAACAACTCGCTAGTCATGGAAGCTCGATGAGGACAACAAAAATCTTTCCTGCTGAAGAACTTGAGAAGGCAACAAACAACTACCATGAGAGTAGAGTCCTTGGAGAAGGAGGTTATGGAACAGTTTACAAAGGAATACTACCGGATGACACAATGGTTGCCATAAAGAAGTCGAAAGGTGGTGCCCTGACCCAGAGTGACCAATTTGTTAACGAGGTGATTGTTCTTTCTCAAATCAACCACAAAAATGTGGTAAAGTTATTGGGTTGTTGTTTAGAAACAGAAGTACCTTTACTAGTATACGAATTCATTACCCATGGCACTCTTTATGAGCACATTCATAAAAAAGGATCAACACTCCCATTTGAATTACGAATGAAGATAGCAACTCAAAGCGCAGAAGCACTGTCCCACTTACACTCCTCAATTTCCACACCAATAATACATCGAGATGTGAAAACAGCAAATATTCTGCTAGATGATGATTATACAGCAAAAGTGTCAGATTTTGGAGCTTCCCGATTGGTTCTTTCTGGTCAAACTGATATACAAACTTTAGTGCTTGGGACATTTGGATACCTGGACCCTGAATATCTGCAATCAAACCAACTAACAGAAAAGAGTGATGTTTATAGCTTTGGAGTTGTCCTAGTGGAGCTACTAACAAGCAAAATGCCTGTTTCTAAAGATAGATGCTTAGCAAGTATCTTTCTTGCTTCCATGGAAGAAGGATGGCTGAATCAAATTCTTGATGATGGCATAGTGAATGAGGGAAACATTGAGACGGTAAAAAAAGTGGCCAATCTCGCAAAACGATGCTTGAGGGTAAACGGGGAGGAAAGGCCTACCATGAAAGAAGTTGCGAAAGAGTTAGAGGAAAAGAGTGTCACGGCAAAATATCCGTGGGGATTTAATGCTAATTTTTGCGAAGAAGAGAATGAATACTTGCTTGGGTCAGTTAATTCAGACGCCTACGTTGTGGGTAATGGAGAAGGTGATTGCAGTTCTAGTGGTCTAACCGGTGGTACGACCACTGCGTATGGCAGCATGCAAATCGAACAGTTAATGTTACATGATGGTGGACGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

760

Amino Acids

83.22

Weight (kDa)

5.71

Isoelectric Point (pI)

33.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 36 - 96 8.7e-11 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 426 - 687 1.4e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 429 - 689 1.4e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 177
AccB1I GGYRCC 1 cut(s) 1376
AccI GTMKAC 1 cut(s) 1494
AciI CCGC 5 cut(s) 32, 56, 120, 420, 698
AclWI GGATC 3 cut(s) 330, 544, 1552
AcoI YGGCCR 1 cut(s) 1992
AcsI RAATTY 4 cut(s) 617, 1104, 1499, 1938
AcuI CTGAAG 1 cut(s) 1269
AcyI GRCGYC 1 cut(s) 2163
AfaI GTAC 4 cut(s) 64, 869, 1482, 2221
AfiI CCNNNNNNNGG 4 cut(s) 31, 52, 471, 1057
AflIII ACRYGT 1 cut(s) 1115
AgeI ACCGGT 1 cut(s) 2213
AgsI TTSAA 5 cut(s) 598, 664, 913, 1168, 1562
AhlI ACTAGT 1 cut(s) 1489
AjnI CCWGG 3 cut(s) 847, 946, 1773
AjuI GAANNNNNNNTTGG 2 cut(s) 2127, 2159
Alw21I GWGCWC 1 cut(s) 1530
Alw26I GTCTC 2 cut(s) 536, 1970
AlwI GGATC 3 cut(s) 330, 544, 1552
AlwNI CAGNNNCTG 2 cut(s) 80, 83
AoxI GGCC 6 cut(s) 12, 720, 905, 991, 1992, 2036
ApeKI GCWGC 6 cut(s) 77, 80, 83, 422, 630, 2238
ApoI RAATTY 4 cut(s) 617, 1104, 1499, 1938
ArsI GACNNNNNNTTYG 2 cut(s) 440, 472
AseI ATTAAT 1 cut(s) 813
AsiGI ACCGGT 1 cut(s) 2213
AspLEI GCGC 1 cut(s) 1592
AspS9I GGNCC 4 cut(s) 323, 462, 720, 1777
AsuHPI GGTGA 5 cut(s) 284, 343, 661, 1423, 2201
AvaII GGWCC 3 cut(s) 323, 462, 1777
BaeGI GKGCMC 1 cut(s) 1381
BaeI ACNNNNGTAYC 2 cut(s) 1464, 1497
BalI TGGCCA 1 cut(s) 1994
BanI GGYRCC 1 cut(s) 1376
BarI GAAGNNNNNNTAC 4 cut(s) 149, 181, 1470, 1502
Bbv12I GWGCWC 1 cut(s) 1530
BbvI GCAGC 6 cut(s) 67, 70, 89, 434, 642, 2250
BccI CCATC 4 cut(s) 872, 1920, 1943, 2264
BceAI ACGGC 3 cut(s) 386, 1006, 2100
BcgI CGANNNNNNTGC 2 cut(s) 2230, 2264
BciT130I CCWGG 3 cut(s) 849, 948, 1775
BciVI GTATCC 2 cut(s) 971, 1763
BcoDI GTCTC 2 cut(s) 536, 1970
BcuI ACTAGT 1 cut(s) 1489
BfaI CTAG 7 cut(s) 60, 678, 1206, 1490, 1670, 1844, 2202
BfmI CTRYAG 1 cut(s) 628
BfuAI ACCTGC 1 cut(s) 177
BfuI GTATCC 2 cut(s) 971, 1763
BisI GCNGC 9 cut(s) 33, 57, 78, 81, 84, 121, 423, 631, 2239
BlpI GCTNAGC 1 cut(s) 1891
BlsI GCNGC 9 cut(s) 34, 58, 79, 82, 85, 122, 424, 632, 2240
BmcAI AGTACT 1 cut(s) 64
Bme1390I CCNGG 3 cut(s) 849, 948, 1775
Bme18I GGWCC 3 cut(s) 323, 462, 1777
BmgT120I GGNCC 4 cut(s) 323, 462, 720, 1777
BmiI GGNNCC 2 cut(s) 1378, 1779
BmrFI CCNGG 3 cut(s) 849, 948, 1775
BmrI ACTGGG 2 cut(s) 243, 549
BmsI GCATC 5 cut(s) 304, 1020, 1092, 1877, 2000
BmuI ACTGGG 2 cut(s) 243, 549
Bpu1102I GCTNAGC 1 cut(s) 1891
BpuEI CTTGAG 2 cut(s) 1277, 2035
BsaHI GRCGYC 1 cut(s) 2163
BsaJI CCNNGG 8 cut(s) 430, 439, 496, 1099, 1294, 1510, 1914, 2096
BsaWI WCCGGW 3 cut(s) 762, 1336, 2213
BsaXI ACNNNNNCTCC 2 cut(s) 1598, 1628
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 52, 471, 1057
Bse118I RCCGGY 1 cut(s) 2213
Bse1I ACTGG 4 cut(s) 238, 296, 555, 838
Bse3DI GCAATG 2 cut(s) 396, 642
BseBI CCWGG 3 cut(s) 849, 948, 1775
BseDI CCNNGG 8 cut(s) 430, 439, 496, 1099, 1294, 1510, 1914, 2096
BseGI GGATG 2 cut(s) 1345, 1931
BseLI CCNNNNNNNGG 4 cut(s) 31, 52, 471, 1057
BseMI GCAATG 2 cut(s) 396, 642
BseMII CTCAG 1 cut(s) 569
BseNI ACTGG 4 cut(s) 238, 296, 555, 838
BseRI GAGGAG 1 cut(s) 1606
BseSI GKGCMC 1 cut(s) 1381
BseXI GCAGC 6 cut(s) 67, 70, 89, 434, 642, 2250
BseYI CCCAGC 1 cut(s) 97
BsgI GTGCAG 2 cut(s) 548, 922
Bsh1285I CGRYCG 1 cut(s) 458
BshFI GGCC 6 cut(s) 14, 722, 907, 993, 1994, 2038
BshNI GGYRCC 1 cut(s) 1376
BshTI ACCGGT 1 cut(s) 2213
BsiEI CGRYCG 1 cut(s) 458
BsiHKAI GWGCWC 1 cut(s) 1530
BsiSI CCGG 3 cut(s) 763, 1337, 2214
BslFI GGGAC 2 cut(s) 1588, 1774
BslI CCNNNNNNNGG 4 cut(s) 31, 52, 471, 1057
BsmAI GTCTC 2 cut(s) 536, 1970
BsmBI CGTCTC 1 cut(s) 1970
BsmFI GGGAC 2 cut(s) 1588, 1774
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 6 cut(s) 14, 722, 907, 993, 1994, 2038
Bsp1286I GDGCHC 2 cut(s) 1381, 1530
Bsp143I GATC 6 cut(s) 6, 335, 372, 549, 1041, 1544
Bsp1720I GCTNAGC 1 cut(s) 1891
Bsp19I CCATGG 2 cut(s) 1510, 1914
BspACI CCGC 5 cut(s) 32, 56, 120, 420, 698
BspANI GGCC 6 cut(s) 14, 722, 907, 993, 1994, 2038
BspCNI CTCAG 1 cut(s) 570
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 2 cut(s) 1378, 1779
BspMAI CTGCAG 1 cut(s) 632
BspMI ACCTGC 1 cut(s) 177
BspPI GGATC 3 cut(s) 330, 544, 1552
BspT107I GGYRCC 1 cut(s) 1376
BsrDI GCAATG 2 cut(s) 396, 642
BsrFI RCCGGY 1 cut(s) 2213
BsrI ACTGG 4 cut(s) 238, 296, 555, 838
BssAI RCCGGY 1 cut(s) 2213
BssECI CCNNGG 8 cut(s) 430, 439, 496, 1099, 1294, 1510, 1914, 2096
BssMI GATC 6 cut(s) 6, 335, 372, 549, 1041, 1544
BssNAI GTATAC 1 cut(s) 1495
BssNI GRCGYC 1 cut(s) 2163
BssT1I CCWWGG 6 cut(s) 430, 496, 1099, 1294, 1510, 1914
Bst1107I GTATAC 1 cut(s) 1495
Bst2UI CCWGG 3 cut(s) 849, 948, 1775
Bst4CI ACNGT 7 cut(s) 806, 933, 1078, 1318, 1602, 1981, 2256
Bst6I CTCTTC 3 cut(s) 366, 679, 2121
BstACI GRCGYC 1 cut(s) 2163
BstAPI GCANNNNNTGC 1 cut(s) 83
BstC8I GCNNGC 4 cut(s) 778, 845, 905, 2243
BstDEI CTNAG 3 cut(s) 546, 578, 1891
BstDSI CCRYGG 3 cut(s) 1510, 1914, 2096
BstF5I GGATG 2 cut(s) 1345, 1931
BstHHI GCGC 1 cut(s) 1592
BstKTI GATC 6 cut(s) 9, 338, 375, 552, 1044, 1547
BstMAI GTCTC 2 cut(s) 536, 1970
BstMBI GATC 6 cut(s) 6, 335, 372, 549, 1041, 1544
BstMCI CGRYCG 1 cut(s) 458
BstMWI GCNNNNNNNGC 5 cut(s) 83, 355, 644, 782, 2000
BstNI CCWGG 3 cut(s) 849, 948, 1775
BstNSI RCATGY 4 cut(s) 481, 780, 1119, 2245
BstSCI CCNGG 3 cut(s) 847, 946, 1773
BstSFI CTRYAG 1 cut(s) 628
BstSLI GKGCMC 1 cut(s) 1381
BstV1I GCAGC 6 cut(s) 67, 70, 89, 434, 642, 2250
BstX2I RGATCY 2 cut(s) 335, 549
BstXI CCANNNNNNTGG 1 cut(s) 878
BstYI RGATCY 2 cut(s) 335, 549
BstZ17I GTATAC 1 cut(s) 1495
BsuI GTATCC 2 cut(s) 971, 1763
BsuRI GGCC 6 cut(s) 14, 722, 907, 993, 1994, 2038
BtgI CCRYGG 3 cut(s) 1510, 1914, 2096
BtsCI GGATG 2 cut(s) 1345, 1931
BtsI GCAGTG 2 cut(s) 508, 2226
BtsIMutI CAGTG 7 cut(s) 508, 562, 647, 845, 938, 1598, 2226
BveI ACCTGC 1 cut(s) 177
Cac8I GCNNGC 4 cut(s) 778, 845, 905, 2243
CaiI CAGNNNCTG 2 cut(s) 80, 83
CciI TCATGA 1 cut(s) 265
CfoI GCGC 1 cut(s) 1592
Cfr10I RCCGGY 1 cut(s) 2213
Cfr13I GGNCC 4 cut(s) 323, 462, 720, 1777
CseI GACGC 1 cut(s) 2171
Csp6I GTAC 4 cut(s) 63, 868, 1481, 2220
CspAI ACCGGT 1 cut(s) 2213
CviQI GTAC 4 cut(s) 63, 868, 1481, 2220
DdeI CTNAG 3 cut(s) 546, 578, 1891
DpnI GATC 6 cut(s) 8, 337, 374, 551, 1043, 1546
DpnII GATC 6 cut(s) 6, 335, 372, 549, 1041, 1544
EaeI YGGCCR 1 cut(s) 1992
Eam1104I CTCTTC 3 cut(s) 366, 679, 2121
EarI CTCTTC 3 cut(s) 366, 679, 2121
Eco130I CCWWGG 6 cut(s) 430, 496, 1099, 1294, 1510, 1914
Eco147I AGGCCT 2 cut(s) 907, 2038
Eco47I GGWCC 3 cut(s) 323, 462, 1777
Eco57I CTGAAG 1 cut(s) 1269
EcoO109I RGGNCCY 1 cut(s) 462
EcoRI GAATTC 1 cut(s) 1499
EcoRII CCWGG 3 cut(s) 847, 946, 1773
EcoT14I CCWWGG 6 cut(s) 430, 496, 1099, 1294, 1510, 1914
EcoT22I ATGCAT 2 cut(s) 319, 782
ErhI CCWWGG 6 cut(s) 430, 496, 1099, 1294, 1510, 1914
Esp3I CGTCTC 1 cut(s) 1970
FaqI GGGAC 2 cut(s) 1588, 1774
FauNDI CATATG 1 cut(s) 319
FblI GTMKAC 1 cut(s) 1494
Fnu4HI GCNGC 9 cut(s) 33, 57, 78, 81, 84, 121, 423, 631, 2239
FokI GGATG 2 cut(s) 1352, 1938
Fsp4HI GCNGC 9 cut(s) 33, 57, 78, 81, 84, 121, 423, 631, 2239
FspBI CTAG 7 cut(s) 60, 678, 1206, 1490, 1670, 1844, 2202
GlaI GCGC 1 cut(s) 1591
GluI GCNGC 9 cut(s) 33, 57, 78, 81, 84, 121, 423, 631, 2239
GsaI CCCAGC 1 cut(s) 101
HaeIII GGCC 6 cut(s) 14, 722, 907, 993, 1994, 2038
HapII CCGG 3 cut(s) 763, 1337, 2214
HgaI GACGC 1 cut(s) 2171
HhaI GCGC 1 cut(s) 1592
Hin1I GRCGYC 1 cut(s) 2163
Hin6I GCGC 1 cut(s) 1590
HinP1I GCGC 1 cut(s) 1590
HincII GTYRAC 1 cut(s) 1405
HindII GTYRAC 1 cut(s) 1405
HinfI GANTC 4 cut(s) 238, 1022, 1290, 1933
HpaI GTTAAC 1 cut(s) 1405
HpaII CCGG 3 cut(s) 763, 1337, 2214
HphI GGTGA 5 cut(s) 284, 343, 661, 1423, 2201
Hpy166II GTNNAC 7 cut(s) 560, 1113, 1321, 1405, 1495, 2023, 2276
Hpy188I TCNGA 6 cut(s) 379, 579, 684, 1027, 1699, 2161
Hpy188III TCNNGA 4 cut(s) 266, 1643, 1713, 1943
Hpy8I GTNNAC 7 cut(s) 560, 1113, 1321, 1405, 1495, 2023, 2276
Hpy99I CGWCG 1 cut(s) 992
HpyAV CCTTC 9 cut(s) 151, 263, 272, 649, 851, 1255, 1295, 1916, 2180
HpyCH4III ACNGT 7 cut(s) 806, 933, 1078, 1318, 1602, 1981, 2256
HpyCH4IV ACGT 1 cut(s) 2169
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 355, 644, 782, 2000
HpyF3I CTNAG 3 cut(s) 546, 578, 1891
HpySE526I ACGT 1 cut(s) 2169
Hsp92I GRCGYC 1 cut(s) 2163
HspAI GCGC 1 cut(s) 1590
KspAI GTTAAC 1 cut(s) 1405
Kzo9I GATC 6 cut(s) 6, 335, 372, 549, 1041, 1544
LmnI GCTCC 2 cut(s) 1706, 1849
Lsp1109I GCAGC 6 cut(s) 67, 70, 89, 434, 642, 2250
LweI GCATC 5 cut(s) 304, 1020, 1092, 1877, 2000
MaeI CTAG 7 cut(s) 60, 678, 1206, 1490, 1670, 1844, 2202
MaeII ACGT 1 cut(s) 2169
MalI GATC 6 cut(s) 8, 337, 374, 551, 1043, 1546
MboI GATC 6 cut(s) 6, 335, 372, 549, 1041, 1544
MboII GAAGA 9 cut(s) 166, 353, 666, 1141, 1262, 1585, 1931, 2135, 2138
MfeI CAATTG 2 cut(s) 715, 793
MflI RGATCY 2 cut(s) 335, 549
MhlI GDGCHC 2 cut(s) 1381, 1530
MlsI TGGCCA 1 cut(s) 1994
MluNI TGGCCA 1 cut(s) 1994
MlyI GAGTC 1 cut(s) 1299
MmeI TCCRAC 1 cut(s) 350
Mox20I TGGCCA 1 cut(s) 1994
Mph1103I ATGCAT 2 cut(s) 319, 782
MscI TGGCCA 1 cut(s) 1994
MslI CAYNNNNRTG 7 cut(s) 270, 606, 813, 876, 1440, 1644, 2271
Msp20I TGGCCA 1 cut(s) 1994
MspA1I CMGCKG 2 cut(s) 80, 101
MspI CCGG 3 cut(s) 763, 1337, 2214
MspR9I CCNGG 3 cut(s) 849, 948, 1775
MunI CAATTG 2 cut(s) 715, 793
Mva1269I GAATGC 1 cut(s) 179
MvaI CCWGG 3 cut(s) 849, 948, 1775
MwoI GCNNNNNNNGC 5 cut(s) 83, 355, 644, 782, 2000
NcoI CCATGG 2 cut(s) 1510, 1914
NdeI CATATG 1 cut(s) 319
NdeII GATC 6 cut(s) 6, 335, 372, 549, 1041, 1544
NlaIV GGNNCC 2 cut(s) 1378, 1779
NmeAIII GCCGAG 1 cut(s) 911
NmuCI GTSAC 3 cut(s) 968, 1391, 2080
NsiI ATGCAT 2 cut(s) 319, 782
NspI RCATGY 4 cut(s) 481, 780, 1119, 2245
PaeI GCATGC 2 cut(s) 780, 2245
PagI TCATGA 1 cut(s) 265
PceI AGGCCT 2 cut(s) 907, 2038
PciI ACATGT 1 cut(s) 1115
PcsI WCGNNNNNNNCGW 1 cut(s) 2228
PctI GAATGC 1 cut(s) 179
PfeI GAWTC 3 cut(s) 238, 1022, 1933
PfoI TCCNGGA 1 cut(s) 946
PinAI ACCGGT 1 cut(s) 2213
PkrI GCNGC 9 cut(s) 34, 58, 79, 82, 85, 122, 424, 632, 2240
PleI GAGTC 1 cut(s) 1298
PpsI GAGTC 1 cut(s) 1298
PpuMI RGGWCCY 1 cut(s) 462
PscI ACATGT 1 cut(s) 1115
PshBI ATTAAT 1 cut(s) 813
Psp5II RGGWCCY 1 cut(s) 462
Psp6I CCWGG 3 cut(s) 847, 946, 1773
PspFI CCCAGC 1 cut(s) 97
PspGI CCWGG 3 cut(s) 847, 946, 1773
PspN4I GGNNCC 2 cut(s) 1378, 1779
PspPI GGNCC 4 cut(s) 323, 462, 720, 1777
PspPPI RGGWCCY 1 cut(s) 462
PstI CTGCAG 1 cut(s) 632
PstNI CAGNNNCTG 2 cut(s) 80, 83
PsuI RGATCY 2 cut(s) 335, 549
PvuII CAGCTG 2 cut(s) 80, 101
RsaI GTAC 4 cut(s) 64, 869, 1482, 2221
RsaNI GTAC 4 cut(s) 63, 868, 1481, 2220
RseI CAYNNNNRTG 7 cut(s) 270, 606, 813, 876, 1440, 1644, 2271
SatI GCNGC 9 cut(s) 33, 57, 78, 81, 84, 121, 423, 631, 2239
Sau3AI GATC 6 cut(s) 6, 335, 372, 549, 1041, 1544
Sau96I GGNCC 4 cut(s) 323, 462, 720, 1777
ScaI AGTACT 1 cut(s) 64
SchI GAGTC 1 cut(s) 1299
ScrFI CCNGG 3 cut(s) 849, 948, 1775
SduI GDGCHC 2 cut(s) 1381, 1530
SfaNI GCATC 5 cut(s) 304, 1020, 1092, 1877, 2000
SfcI CTRYAG 1 cut(s) 628
SinI GGWCC 3 cut(s) 323, 462, 1777
SmiMI CAYNNNNRTG 7 cut(s) 270, 606, 813, 876, 1440, 1644, 2271
SmlI CTYRAG 2 cut(s) 1256, 2014
SmoI CTYRAG 2 cut(s) 1256, 2014
SpeI ACTAGT 1 cut(s) 1489
SphI GCATGC 2 cut(s) 780, 2245
SseBI AGGCCT 2 cut(s) 907, 2038
SsiI CCGC 5 cut(s) 32, 56, 120, 420, 698
SspI AATATT 3 cut(s) 285, 943, 1663
SspMI CTAG 7 cut(s) 60, 678, 1206, 1490, 1670, 1844, 2202
StuI AGGCCT 2 cut(s) 907, 2038
StyD4I CCNGG 3 cut(s) 847, 946, 1773
StyI CCWWGG 6 cut(s) 430, 496, 1099, 1294, 1510, 1914
TaaI ACNGT 7 cut(s) 806, 933, 1078, 1318, 1602, 1981, 2256
TaiI ACGT 1 cut(s) 2172
TaqI TCGA 6 cut(s) 5, 458, 1220, 1367, 1642, 2250
TaqII GACCGA 1 cut(s) 444
TatI WGTACW 1 cut(s) 62
TauI GCSGC 3 cut(s) 35, 59, 123
TfiI GAWTC 3 cut(s) 238, 1022, 1933
TscAI CASTG 7 cut(s) 508, 562, 654, 845, 938, 1605, 2233
TseFI GTSAC 3 cut(s) 968, 1391, 2080
TseI GCWGC 6 cut(s) 77, 80, 83, 422, 630, 2238
Tsp45I GTSAC 3 cut(s) 968, 1391, 2080
TspGWI ACGGA 3 cut(s) 782, 942, 2085
TspRI CASTG 7 cut(s) 508, 562, 654, 845, 938, 1605, 2233
VpaK11BI GGWCC 3 cut(s) 323, 462, 1777
VspI ATTAAT 1 cut(s) 813
XapI RAATTY 4 cut(s) 617, 1104, 1499, 1938
XceI RCATGY 4 cut(s) 481, 780, 1119, 2245
XcmI CCANNNNNNNNNTGG 1 cut(s) 845
XmiI GTMKAC 1 cut(s) 1494
XspI CTAG 7 cut(s) 60, 678, 1206, 1490, 1670, 1844, 2202
ZrmI AGTACT 1 cut(s) 64
Zsp2I ATGCAT 2 cut(s) 319, 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.