Rroxscaffold_1G00026170

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000066
Physical Location & Seq
Reverse (-)
32924994 .. 32927737
2744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00026170.1

Sequence Viewer

Length: 1914 bp
ATGGCCTTACACGGGAAATTGATGCTAGCTGTGCAACTGATCATTTCATTGTTACCAGTGGCCATAGCAACAGCAGCAGCTACTGATCAAATCCTGCCTCCTCTAGCCCTGGCTGGCTGCCCTGACCACTGTGGTAATCTCACAATCCCATATCCTTTCGGCATCGGGGAGGGTTGTTTCTTGCAGAATTGGTTCAACCTCACTTGTAACGAATCCGCCGAACCCCAAAGAACACTCTGGGGCGATGGTAGTATCACGGTTACTAACATATTGCTTGCTGAAGGTGAGTTGGAGATTTTGAATTACATAGCCTATGATTGTTATGATCAGCAGGGTAATCAAACAGAAGACATGTACAATTCCCCTTATCTCCGGTTGCATCCTTCTTTCACCATCTCCGGGACCAGAAACAAGTTTATTGCCTTAACGGTTCGTGCTCTGGCATTGGGTGTTGCGAAACTTCCATCCCTAGCGGACTCAAAAATCGCTCCATCAATTTGGATAGCTATTCTCAACACAAGGACGTGTGGGGCTTCAATCCATGCAGCTACGCTTTCATTGTGGAAGAAGGCAGGGTCATGTGCTCTAGCTCAAAACAGGTCTGGTTATGCTTGCAAAGCAAATAGCAGCTGTGTCAATCGGACCGTCGATTACCAGACTGAACCGACTGGTTATTATTGCCAGTGCTTGCCAGGCTTCGAAGGGAACCCATATCTTCCAGATGGTTGCCAAGGACTCAAAGAATTGCATAGAAAACACTCCCGTGCCAAAGAACATGCCCCAGAAAATTTCGTTGGGTCTCAGTATAGGCTTCTTCATTTTAGTGGTTTTAATTTGGTGGACTTATTGGGGAATGAACAAACGAAGACTAAGGAACTTCAGAAAGCCACAAACAATTACCATGTAGATGAAATCCTTAGTGAAGGAGGCTACGCCACAGTTTATAAAGGAATACTACATGATAAGAGTGTTGTTGCCATAAAGAAGCCAAAGATTGGCGCTCCAACTCAAAGTGATCAGTTCGTTAATGAGGTTATCGTTCTTTCTCAGATAAACCACAGAAATGTAGTAAAACTATTAGGTTGTTGTTTCGAGACCGAAGTGCCTTTACTGGTATATGAGTACATTACTCATGGCACTCTTTTCGAGCGTGTATTCAATAAGGGAAAAGGATCCTCGCTTTCATGGGAATTACGACTGAAGATAGCATCAGAAACTGCTGACGCACTAGCATACTTACACTCATCTACTTCCACTCCAATCATACATAGAGACGTGAAAACAACGAATATATTATTGGATGACAATTACACGGCCAAAGTGTCTGATTTTGGAGCTTCGCGTTTCATTCCTATAGATCAAACTCAACTTGCAACACTAGTTCAAGGGACACTTGGATACTTGGATCCTGAATACTTTCATTCAAACCAACTAACTGAAAAGAGTGATGTCTATAGTTTTGGAGTTGTCCTAGCGGAGCTACTAACAAGTAGAGTTGCACTTTCTTTTGCCAGGCCTGACAAGGAGAGATGCCTAGCAAGCTTCTTTGTTTCTTCAATAGAAAACGAATGCTTGATTCAAATTCTTGATGCTGATATAGTGAATGAGGGGAACATAGAGACAGTAGAGCAAGTGGCGAATCTGGCAAAAAGATGTTTGATGATAAAAAGTGGGGAGAGACCTACGATGAAAGAAGTTGCCATGGAGTTAGAAGGGATGAGAATTATGGCAATGCATCCATGGAAAAATGGCAATTCCGGCTTTGAGGAGACTGAGTACTTGCTCGGGTCACCTAATTCAGCGGCTTATGTGATGAATATTGGAGATGGAGGTGCCGGTGGTTCGAGCGTTACAAATACTGGGTATGACAGCATGCAAATTCAAATGTTAATGCCCTACGATGACGGGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

637

Amino Acids

69.98

Weight (kDa)

5.57

Isoelectric Point (pI)

34.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 40 - 99 8.2e-12 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 300 - 566 2.1e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 302 - 570 1.9e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 945
AccB1I GGYRCC 1 cut(s) 1832
AccB7I CCANNNNNTGG 1 cut(s) 995
AccII CGCG 1 cut(s) 1342
AciI CCGC 4 cut(s) 216, 473, 1475, 1802
AclWI GGATC 4 cut(s) 1167, 1180, 1400, 1413
AcoI YGGCCR 2 cut(s) 60, 1314
AcsI RAATTY 3 cut(s) 787, 1581, 1878
AcuI CTGAAG 3 cut(s) 300, 863, 1220
AfaI GTAC 3 cut(s) 356, 1124, 1778
AfiI CCNNNNNNNGG 3 cut(s) 12, 399, 995
AflIII ACRYGT 2 cut(s) 351, 524
AgsI TTSAA 9 cut(s) 196, 301, 537, 1159, 1385, 1425, 1557, 1580, 1883
AhlI ACTAGT 1 cut(s) 1378
AjiI CACGTC 2 cut(s) 525, 1276
AjnI CCWGG 3 cut(s) 108, 691, 1511
AjuI GAANNNNNNNTTGG 4 cut(s) 777, 809, 1280, 1312
AleI CACNNNNGTG 1 cut(s) 762
AluBI AGCT 9 cut(s) 29, 80, 506, 548, 590, 630, 1337, 1480, 1542
AluI AGCT 9 cut(s) 29, 80, 506, 548, 590, 630, 1337, 1480, 1542
Alw21I GWGCWC 2 cut(s) 439, 586
Alw26I GTCTC 6 cut(s) 804, 1088, 1266, 1613, 1672, 1763
AlwI GGATC 4 cut(s) 1167, 1180, 1400, 1413
AlwNI CAGNNNCTG 2 cut(s) 83, 1217
Ama87I CYCGRG 1 cut(s) 1784
AoxI GGCC 4 cut(s) 3, 60, 1314, 1514
ApeKI GCWGC 5 cut(s) 74, 77, 117, 545, 627
ApoI RAATTY 3 cut(s) 787, 1581, 1878
Asp700I GAANNNNTTC 2 cut(s) 191, 1416
AspLEI GCGC 1 cut(s) 1001
AspS9I GGNCC 2 cut(s) 402, 642
AsuC2I CCSGG 1 cut(s) 400
AsuHPI GGTGA 3 cut(s) 296, 382, 1782
AsuII TTCGAA 1 cut(s) 699
AsuNHI GCTAGC 1 cut(s) 25
AvaI CYCGRG 1 cut(s) 1784
AvaII GGWCC 2 cut(s) 402, 642
BalI TGGCCA 1 cut(s) 62
BamHI GGATCC 2 cut(s) 1172, 1405
BanI GGYRCC 1 cut(s) 1832
BarI GAAGNNNNNNTAC 2 cut(s) 1092, 1124
BbsI GAAGAC 2 cut(s) 354, 872
Bbv12I GWGCWC 2 cut(s) 439, 586
BbvI GCAGC 5 cut(s) 86, 89, 104, 557, 639
BccI CCATC 6 cut(s) 239, 401, 472, 499, 716, 1820
BceAI ACGGC 1 cut(s) 1329
BcgI CGANNNNNNTGC 2 cut(s) 1126, 1160
BciT130I CCWGG 3 cut(s) 110, 693, 1513
BciVI GTATCC 1 cut(s) 1391
BclI TGATCA 4 cut(s) 39, 85, 325, 1015
BcnI CCSGG 1 cut(s) 400
BcoDI GTCTC 6 cut(s) 804, 1088, 1266, 1613, 1672, 1763
BcuI ACTAGT 1 cut(s) 1378
BfaI CTAG 8 cut(s) 26, 104, 470, 587, 1229, 1379, 1472, 1535
BfmI CTRYAG 2 cut(s) 1353, 1453
BfoI RGCGCY 1 cut(s) 1002
BfuI GTATCC 1 cut(s) 1391
BisI GCNGC 6 cut(s) 75, 78, 118, 546, 628, 1803
BlsI GCNGC 6 cut(s) 76, 79, 119, 547, 629, 1804
BmcAI AGTACT 1 cut(s) 1778
Bme1390I CCNGG 4 cut(s) 110, 400, 693, 1513
Bme18I GGWCC 2 cut(s) 402, 642
BmeT110I CYCGRG 1 cut(s) 1784
BmgBI CACGTC 2 cut(s) 525, 1276
BmgT120I GGNCC 2 cut(s) 402, 642
BmiI GGNNCC 5 cut(s) 403, 707, 1174, 1407, 1834
BmrFI CCNGG 4 cut(s) 110, 400, 693, 1513
BmrI ACTGGG 1 cut(s) 1869
BmsI GCATC 7 cut(s) 12, 171, 388, 1217, 1520, 1579, 1744
BmtI GCTAGC 1 cut(s) 29
BmuI ACTGGG 1 cut(s) 1869
BpiI GAAGAC 2 cut(s) 354, 872
Bpu14I TTCGAA 1 cut(s) 699
BpuMI CCSGG 1 cut(s) 400
BsaI GGTCTC 3 cut(s) 804, 1088, 1672
BsaJI CCNNGG 4 cut(s) 108, 730, 1701, 1739
BsaWI WCCGGW 1 cut(s) 372
BsaXI ACNNNNNCTCC 4 cut(s) 1240, 1270, 1760, 1790
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 399, 995
Bse118I RCCGGY 1 cut(s) 1835
Bse1I ACTGG 5 cut(s) 56, 673, 682, 1116, 1864
Bse3DI GCAATG 1 cut(s) 1737
BseBI CCWGG 3 cut(s) 110, 693, 1513
BseDI CCNNGG 4 cut(s) 108, 730, 1701, 1739
BseGI GGATG 5 cut(s) 379, 464, 1306, 1722, 1735
BseLI CCNNNNNNNGG 3 cut(s) 12, 399, 995
BseMI GCAATG 1 cut(s) 1737
BseMII CTCAG 3 cut(s) 815, 1061, 1764
BseNI ACTGG 5 cut(s) 56, 673, 682, 1116, 1864
BseRI GAGGAG 2 cut(s) 90, 1781
BseXI GCAGC 5 cut(s) 86, 89, 104, 557, 639
Bsh1236I CGCG 1 cut(s) 1342
BshFI GGCC 4 cut(s) 5, 62, 1316, 1516
BshNI GGYRCC 1 cut(s) 1832
BsiHKAI GWGCWC 2 cut(s) 439, 586
BsiHKCI CYCGRG 1 cut(s) 1784
BsiSI CCGG 4 cut(s) 373, 399, 1758, 1836
BslFI GGGAC 2 cut(s) 415, 1402
BslI CCNNNNNNNGG 3 cut(s) 12, 399, 995
BsmAI GTCTC 6 cut(s) 804, 1088, 1266, 1613, 1672, 1763
BsmBI CGTCTC 1 cut(s) 1266
BsmFI GGGAC 2 cut(s) 415, 1402
BsmI GAATGC 1 cut(s) 1574
BsnI GGCC 4 cut(s) 5, 62, 1316, 1516
Bso31I GGTCTC 3 cut(s) 804, 1088, 1672
BsoBI CYCGRG 1 cut(s) 1784
Bsp119I TTCGAA 1 cut(s) 699
Bsp1286I GDGCHC 2 cut(s) 439, 586
Bsp1407I TGTACA 1 cut(s) 354
Bsp143I GATC 7 cut(s) 39, 85, 325, 1015, 1172, 1357, 1405
Bsp19I CCATGG 2 cut(s) 1701, 1739
BspACI CCGC 4 cut(s) 216, 473, 1475, 1802
BspANI GGCC 4 cut(s) 5, 62, 1316, 1516
BspCNI CTCAG 3 cut(s) 814, 1060, 1765
BspFNI CGCG 1 cut(s) 1342
BspLI GGNNCC 5 cut(s) 403, 707, 1174, 1407, 1834
BspOI GCTAGC 1 cut(s) 29
BspPI GGATC 4 cut(s) 1167, 1180, 1400, 1413
BspT104I TTCGAA 1 cut(s) 699
BspT107I GGYRCC 1 cut(s) 1832
BspTNI GGTCTC 3 cut(s) 804, 1088, 1672
BsrDI GCAATG 1 cut(s) 1737
BsrFI RCCGGY 1 cut(s) 1835
BsrGI TGTACA 1 cut(s) 354
BsrI ACTGG 5 cut(s) 56, 673, 682, 1116, 1864
BssAI RCCGGY 1 cut(s) 1835
BssECI CCNNGG 4 cut(s) 108, 730, 1701, 1739
BssMI GATC 7 cut(s) 39, 85, 325, 1015, 1172, 1357, 1405
BssT1I CCWWGG 3 cut(s) 730, 1701, 1739
Bst2UI CCWGG 3 cut(s) 110, 693, 1513
Bst4CI ACNGT 6 cut(s) 131, 259, 430, 646, 940, 1624
BstAUI TGTACA 1 cut(s) 354
BstBI TTCGAA 1 cut(s) 699
BstC8I GCNNGC 7 cut(s) 27, 115, 276, 613, 689, 1540, 1874
BstDEI CTNAG 5 cut(s) 801, 870, 917, 1047, 1773
BstDSI CCRYGG 2 cut(s) 1701, 1739
BstEII GGTNACC 1 cut(s) 1788
BstF5I GGATG 5 cut(s) 379, 464, 1306, 1722, 1735
BstFNI CGCG 1 cut(s) 1342
BstH2I RGCGCY 1 cut(s) 1002
BstHHI GCGC 1 cut(s) 1001
BstKTI GATC 7 cut(s) 42, 88, 328, 1018, 1175, 1360, 1408
BstMAI GTCTC 6 cut(s) 804, 1088, 1266, 1613, 1672, 1763
BstMBI GATC 7 cut(s) 39, 85, 325, 1015, 1172, 1357, 1405
BstMWI GCNNNNNNNGC 7 cut(s) 31, 74, 617, 693, 1539, 1643, 1758
BstNI CCWGG 3 cut(s) 110, 693, 1513
BstNSI RCATGY 3 cut(s) 355, 779, 1876
BstPI GGTNACC 1 cut(s) 1788
BstSCI CCNGG 4 cut(s) 108, 398, 691, 1511
BstSFI CTRYAG 2 cut(s) 1353, 1453
BstUI CGCG 1 cut(s) 1342
BstV1I GCAGC 5 cut(s) 86, 89, 104, 557, 639
BstV2I GAAGAC 2 cut(s) 354, 872
BstX2I RGATCY 2 cut(s) 1172, 1405
BstXI CCANNNNNNTGG 1 cut(s) 498
BstYI RGATCY 2 cut(s) 1172, 1405
BsuI GTATCC 1 cut(s) 1391
BsuRI GGCC 4 cut(s) 5, 62, 1316, 1516
BtgI CCRYGG 2 cut(s) 1701, 1739
BtgZI GCGATG 1 cut(s) 258
BtrI CACGTC 2 cut(s) 525, 1276
BtsCI GGATG 5 cut(s) 379, 464, 1306, 1722, 1735
BtsIMutI CAGTG 3 cut(s) 63, 127, 689
Cac8I GCNNGC 7 cut(s) 27, 115, 276, 613, 689, 1540, 1874
CaiI CAGNNNCTG 2 cut(s) 83, 1217
CfoI GCGC 1 cut(s) 1001
Cfr10I RCCGGY 1 cut(s) 1835
Cfr13I GGNCC 2 cut(s) 402, 642
CpoI CGGWCCG 1 cut(s) 642
CseI GACGC 1 cut(s) 1232
Csp6I GTAC 3 cut(s) 355, 1123, 1777
CspI CGGWCCG 1 cut(s) 642
CviQI GTAC 3 cut(s) 355, 1123, 1777
DdeI CTNAG 5 cut(s) 801, 870, 917, 1047, 1773
DpnI GATC 7 cut(s) 41, 87, 327, 1017, 1174, 1359, 1407
DpnII GATC 7 cut(s) 39, 85, 325, 1015, 1172, 1357, 1405
EaeI YGGCCR 2 cut(s) 60, 1314
EciI GGCGGA 1 cut(s) 205
Eco130I CCWWGG 3 cut(s) 730, 1701, 1739
Eco147I AGGCCT 1 cut(s) 1516
Eco31I GGTCTC 3 cut(s) 804, 1088, 1672
Eco47I GGWCC 2 cut(s) 402, 642
Eco57I CTGAAG 3 cut(s) 300, 863, 1220
Eco88I CYCGRG 1 cut(s) 1784
Eco91I GGTNACC 1 cut(s) 1788
EcoO65I GGTNACC 1 cut(s) 1788
EcoRII CCWGG 3 cut(s) 108, 691, 1511
EcoT14I CCWWGG 3 cut(s) 730, 1701, 1739
EcoT22I ATGCAT 1 cut(s) 1737
ErhI CCWWGG 3 cut(s) 730, 1701, 1739
Esp3I CGTCTC 1 cut(s) 1266
FalI AAGNNNNNCTT 2 cut(s) 1377, 1409
FaqI GGGAC 2 cut(s) 415, 1402
FbaI TGATCA 4 cut(s) 39, 85, 325, 1015
Fnu4HI GCNGC 6 cut(s) 75, 78, 118, 546, 628, 1803
FokI GGATG 5 cut(s) 366, 451, 1313, 1722, 1729
Fsp4HI GCNGC 6 cut(s) 75, 78, 118, 546, 628, 1803
FspBI CTAG 8 cut(s) 26, 104, 470, 587, 1229, 1379, 1472, 1535
GlaI GCGC 1 cut(s) 1000
GluI GCNGC 6 cut(s) 75, 78, 118, 546, 628, 1803
HaeII RGCGCY 1 cut(s) 1002
HaeIII GGCC 4 cut(s) 5, 62, 1316, 1516
HapII CCGG 4 cut(s) 373, 399, 1758, 1836
HgaI GACGC 1 cut(s) 1232
HhaI GCGC 1 cut(s) 1001
Hin6I GCGC 1 cut(s) 999
HinP1I GCGC 1 cut(s) 999
HindIII AAGCTT 1 cut(s) 1540
HinfI GANTC 5 cut(s) 212, 476, 735, 1576, 1639
HpaII CCGG 4 cut(s) 373, 399, 1758, 1836
HphI GGTGA 3 cut(s) 296, 382, 1782
Hpy166II GTNNAC 1 cut(s) 841
Hpy188I TCNGA 5 cut(s) 642, 882, 1050, 1213, 1327
Hpy188III TCNNGA 4 cut(s) 719, 1093, 1409, 1586
Hpy8I GTNNAC 1 cut(s) 841
Hpy99I CGWCG 1 cut(s) 650
HpyAV CCTTC 6 cut(s) 275, 393, 562, 695, 917, 1706
HpyCH4III ACNGT 6 cut(s) 131, 259, 430, 646, 940, 1624
HpyCH4IV ACGT 2 cut(s) 524, 1275
HpyF10VI GCNNNNNNNGC 7 cut(s) 31, 74, 617, 693, 1539, 1643, 1758
HpyF3I CTNAG 5 cut(s) 801, 870, 917, 1047, 1773
HpySE526I ACGT 2 cut(s) 524, 1275
HspAI GCGC 1 cut(s) 999
Ksp22I TGATCA 4 cut(s) 39, 85, 325, 1015
Kzo9I GATC 7 cut(s) 39, 85, 325, 1015, 1172, 1357, 1405
LmnI GCTCC 4 cut(s) 493, 1006, 1334, 1477
Lsp1109I GCAGC 5 cut(s) 86, 89, 104, 557, 639
LweI GCATC 7 cut(s) 12, 171, 388, 1217, 1520, 1579, 1744
MaeI CTAG 8 cut(s) 26, 104, 470, 587, 1229, 1379, 1472, 1535
MaeII ACGT 2 cut(s) 524, 1275
MaeIII GTNAC 5 cut(s) 51, 206, 259, 1788, 1849
MalI GATC 7 cut(s) 41, 87, 327, 1017, 1174, 1359, 1407
MboI GATC 7 cut(s) 39, 85, 325, 1015, 1172, 1357, 1405
MboII GAAGA 7 cut(s) 359, 577, 707, 806, 877, 1213, 1545
MflI RGATCY 2 cut(s) 1172, 1405
MhlI GDGCHC 2 cut(s) 439, 586
MlsI TGGCCA 1 cut(s) 62
MluNI TGGCCA 1 cut(s) 62
MlyI GAGTC 2 cut(s) 470, 729
MmeI TCCRAC 2 cut(s) 270, 1028
Mox20I TGGCCA 1 cut(s) 62
Mph1103I ATGCAT 1 cut(s) 1737
MroXI GAANNNNTTC 2 cut(s) 191, 1416
MscI TGGCCA 1 cut(s) 62
MseI TTAA 4 cut(s) 425, 831, 1026, 1889
MslI CAYNNNNRTG 4 cut(s) 762, 822, 906, 1062
Msp20I TGGCCA 1 cut(s) 62
MspA1I CMGCKG 2 cut(s) 630, 1802
MspI CCGG 4 cut(s) 373, 399, 1758, 1836
MspR9I CCNGG 4 cut(s) 110, 400, 693, 1513
Mva1269I GAATGC 1 cut(s) 1574
MvaI CCWGG 3 cut(s) 110, 693, 1513
MvnI CGCG 1 cut(s) 1342
MwoI GCNNNNNNNGC 7 cut(s) 31, 74, 617, 693, 1539, 1643, 1758
NciI CCSGG 1 cut(s) 400
NcoI CCATGG 2 cut(s) 1701, 1739
NdeII GATC 7 cut(s) 39, 85, 325, 1015, 1172, 1357, 1405
NheI GCTAGC 1 cut(s) 25
NlaIV GGNNCC 5 cut(s) 403, 707, 1174, 1407, 1834
NmuCI GTSAC 1 cut(s) 1788
NsiI ATGCAT 1 cut(s) 1737
NspI RCATGY 3 cut(s) 355, 779, 1876
NspV TTCGAA 1 cut(s) 699
OliI CACNNNNGTG 1 cut(s) 762
PaeI GCATGC 1 cut(s) 1876
PceI AGGCCT 1 cut(s) 1516
PciI ACATGT 1 cut(s) 351
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PctI GAATGC 1 cut(s) 1574
PdmI GAANNNNTTC 2 cut(s) 191, 1416
PfeI GAWTC 3 cut(s) 212, 1576, 1639
PflMI CCANNNNNTGG 1 cut(s) 995
PfoI TCCNGGA 1 cut(s) 398
PkrI GCNGC 6 cut(s) 76, 79, 119, 547, 629, 1804
PleI GAGTC 2 cut(s) 470, 729
PpsI GAGTC 2 cut(s) 470, 729
PscI ACATGT 1 cut(s) 351
PsiI TTATAA 1 cut(s) 945
Psp6I CCWGG 3 cut(s) 108, 691, 1511
PspEI GGTNACC 1 cut(s) 1788
PspGI CCWGG 3 cut(s) 108, 691, 1511
PspN4I GGNNCC 5 cut(s) 403, 707, 1174, 1407, 1834
PspPI GGNCC 2 cut(s) 402, 642
PstNI CAGNNNCTG 2 cut(s) 83, 1217
PsuI RGATCY 2 cut(s) 1172, 1405
PvuII CAGCTG 1 cut(s) 630
RsaI GTAC 3 cut(s) 356, 1124, 1778
RsaNI GTAC 3 cut(s) 355, 1123, 1777
RseI CAYNNNNRTG 4 cut(s) 762, 822, 906, 1062
Rsr2I CGGWCCG 1 cut(s) 642
RsrII CGGWCCG 1 cut(s) 642
SaqAI TTAA 4 cut(s) 425, 831, 1026, 1889
SatI GCNGC 6 cut(s) 75, 78, 118, 546, 628, 1803
Sau3AI GATC 7 cut(s) 39, 85, 325, 1015, 1172, 1357, 1405
Sau96I GGNCC 2 cut(s) 402, 642
ScaI AGTACT 1 cut(s) 1778
SchI GAGTC 2 cut(s) 470, 729
ScrFI CCNGG 4 cut(s) 110, 400, 693, 1513
SduI GDGCHC 2 cut(s) 439, 586
SfaNI GCATC 7 cut(s) 12, 171, 388, 1217, 1520, 1579, 1744
SfcI CTRYAG 2 cut(s) 1353, 1453
SfuI TTCGAA 1 cut(s) 699
SinI GGWCC 2 cut(s) 402, 642
SmiMI CAYNNNNRTG 4 cut(s) 762, 822, 906, 1062
SpeI ACTAGT 1 cut(s) 1378
SphI GCATGC 1 cut(s) 1876
SseBI AGGCCT 1 cut(s) 1516
SsiI CCGC 4 cut(s) 216, 473, 1475, 1802
SspI AATATT 1 cut(s) 1819
SspMI CTAG 8 cut(s) 26, 104, 470, 587, 1229, 1379, 1472, 1535
StuI AGGCCT 1 cut(s) 1516
StyD4I CCNGG 4 cut(s) 108, 398, 691, 1511
StyI CCWWGG 3 cut(s) 730, 1701, 1739
TaaI ACNGT 6 cut(s) 131, 259, 430, 646, 940, 1624
TaiI ACGT 2 cut(s) 527, 1278
TaqI TCGA 5 cut(s) 648, 699, 1092, 1146, 1844
TaqII GACCGA 1 cut(s) 1112
TatI WGTACW 3 cut(s) 354, 1122, 1776
TauI GCSGC 1 cut(s) 1805
TfiI GAWTC 3 cut(s) 212, 1576, 1639
Tru1I TTAA 4 cut(s) 425, 831, 1026, 1889
Tru9I TTAA 4 cut(s) 425, 831, 1026, 1889
TscAI CASTG 3 cut(s) 63, 134, 689
TseFI GTSAC 1 cut(s) 1788
TseI GCWGC 5 cut(s) 74, 77, 117, 545, 627
Tsp45I GTSAC 1 cut(s) 1788
TspRI CASTG 3 cut(s) 63, 134, 689
Van91I CCANNNNNTGG 1 cut(s) 995
VpaK11BI GGWCC 2 cut(s) 402, 642
XapI RAATTY 3 cut(s) 787, 1581, 1878
XceI RCATGY 3 cut(s) 355, 779, 1876
XmnI GAANNNNTTC 2 cut(s) 191, 1416
XspI CTAG 8 cut(s) 26, 104, 470, 587, 1229, 1379, 1472, 1535
ZrmI AGTACT 1 cut(s) 1778
Zsp2I ATGCAT 1 cut(s) 1737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.