Rroxscaffold_1G00060080

specification of plant organ axis polarity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000066
Physical Location & Seq
Forward (+)
82210202 .. 82212875
2674 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00060080.1

Sequence Viewer

Length: 1971 bp
ATGGCGTTGCGACTCTCATTGGTAGCAGTTCTACTCCTGTTATTGGTGGCTAGTACTACTACAACAACAGCAGCTGCTGCTCAAGCCCTGCCTGGTTGCTCAGACAAGTGCGGCAATCTCACAATTCCATATCCATTTGGTTGTTACCTGCGAGAAGAATTCTTTATCAATTGTAGCAATACCACCCAACCACCAACAGCATATTTAAGAGTCAGCAATATCATTGTTACTAACATTTCCCTTGATGAAGGTGAGTTGCAAATATCCAACAACGTGGCTGAAGCTTGCTATAACGAGCAGGGCAATGTGACATCCAGCAAGGATTATTCGCTCGGCCTGCCCCTTCCTTATACGATATCCGATACCAAAAACAAGCTCTACACCGTTGGTTGTGACACTTATTCAATTTTACAAGGCTTCCTAGGGGAAGATGAGTTCGATACGGGGTGCATTTCCATATGCAACAGGCTTGACAGACTAACATCCCCAGAGGAGCAAGGACAGTTCAATTTCTCGGCTGCAAGTTTAAAGAAACAGGACAACAACTCCTGGCTTCCAGCGGTTCTTAATTGGGCTATTGGGAATGACACAGACTCCTGTGATGAAGCTCAAAAGAGGAAGGATTTCGCTTGCAAGGCTAATAGCAGGTGCGCCAACGGGCCTATCAACGGGTCTGTAGGTTACTTGTGCCAATGCTTACCTGGTTATGAAGGAAACCCCTACCATCCAGATGGTTGCCAAGGAATTTCTGTAGGCCTATTTGTGATTTTATTAGTTGGAAGTTTATGGATGTATTGGGGACTGAAGAAAAGGAAGTTCATAAAACTCAAAGAGAAGTACTTTACAGAAAATGGTGGTTTATTGTTAAAACAAAAACTCACTAGTCAAGGAGGATCTGTTGAGACAACCAAACTATTTACTGCAGAAGAACTTGAGAAGGCCACAAACAATTACCATGAAAGTAGAATCCTTGGTGAAGGAGGGTATGGAACAGTTTACAGAGGCATATTACCAGATAACCAAGTGGTTGCAATAAAAAAGTCCAAAGTTGGTGCCCCGACTCAAACTGATCAGTTTGTTAATGAGGTGATTGTTCTTTCTCAAGTCAACCATAGAAATGTAGTAAGGCTATTAGGTTGTTGTTTAGAGACAGAAGTCCCTTTACTAGTATACGAGTTCATCACCCAGGGCACTCTCTTTGAGCACATTCACAAGAAAAAGGGTAAAGGGTCATCACTTTCGTGGGAACTCCGATTGAAGATAGCATCAGAAACTGCAGGAGCACTAGCATACTTGCACTCCTCCACTTCCACACCAATCATACACCGAGATGTGAAAACAATGAATATACTATTAGATGACAATTACACAGCCAAAGTGTCAGATTTTGGAGCTTCACGTTTCATTCCTATTGATCAAACTCAACTAGCAACAATGGTGCAAGGCACATTTGGATACTTGGACCCTGGATACTTCCATTCAAATCAACTAACAGAAAAGAGTGACGTCTATAGCTTTGGAGTGGTTCTAGCAGAGCTACTCACAAGTAAAGTGGCATTTTCTTTTGCAAGGCCTGAGGCAGAGAGATGCCTTGCACACTTTTTTGTTTGTTCGGTGGAAGATGGTCACTTGAACCAAATTCTATATGATGGCATTATTAATGAAGGAGATATTGACCAGAGAGTAATAGAAAATGTGGCCCATCTTGCAAGAAAATGTTTGAGGGTAAAAGGGGAGGAAAGGCCTACCATGAGAGAAGTAGCCATGGAGCTAGAAGGAATGATTATGACAAAGCATCCACGGGGTTCTGCAGATCATTACTTCCCAGAAGAGACTACGCACTTGCTCGGATCACCTAACACTTCCAAGGATTATGTTGTGAATTTTGATGGTGATGGTGGTCCTGGTACAACCAGTGGGTGTGACAGCATGCAAATCGAAATGTTAATGTCATATGCTGATGGGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase_Tyr

Protein Analysis

656

Amino Acids

72.27

Weight (kDa)

5.44

Isoelectric Point (pI)

29.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 33 - 89 1.1e-06 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 318 - 587 1.9e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 321 - 591 9.8e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 636
AatII GACGTC 1 cut(s) 1509
Acc36I ACCTGC 2 cut(s) 156, 636
AccB1I GGYRCC 1 cut(s) 1052
AccI GTMKAC 1 cut(s) 1170
AciI CCGC 2 cut(s) 111, 560
AclWI GGATC 2 cut(s) 901, 1858
AcsI RAATTY 4 cut(s) 158, 744, 1638, 1882
AcuI CTGAAG 2 cut(s) 300, 824
AcyI GRCGYC 1 cut(s) 1506
AfaI GTAC 3 cut(s) 55, 839, 1909
AfiI CCNNNNNNNGG 2 cut(s) 43, 668
AgsI TTSAA 5 cut(s) 405, 508, 1258, 1482, 1633
AhlI ACTAGT 2 cut(s) 881, 1165
AjnI CCWGG 6 cut(s) 91, 548, 700, 1185, 1465, 1903
AleI CACNNNNGTG 1 cut(s) 1240
AloI GAACNNNNNNTCC 2 cut(s) 419, 451
AluBI AGCT 8 cut(s) 74, 284, 376, 608, 1394, 1515, 1537, 1771
AluI AGCT 8 cut(s) 74, 284, 376, 608, 1394, 1515, 1537, 1771
Alw21I GWGCWC 2 cut(s) 1206, 1285
Alw26I GTCTC 3 cut(s) 896, 1142, 1826
AlwI GGATC 2 cut(s) 901, 1858
AlwNI CAGNNNCTG 3 cut(s) 74, 77, 1274
AoxI GGCC 7 cut(s) 334, 659, 754, 939, 1571, 1698, 1742
ApeKI GCWGC 4 cut(s) 71, 74, 77, 518
ApoI RAATTY 4 cut(s) 158, 744, 1638, 1882
AseI ATTAAT 1 cut(s) 1659
Asp700I GAANNNNTTC 1 cut(s) 623
AspA2I CCTAGG 1 cut(s) 421
AspLEI GCGC 1 cut(s) 653
AspS9I GGNCC 4 cut(s) 659, 1462, 1699, 1901
AsuHPI GGTGA 6 cut(s) 263, 986, 1099, 1174, 1845, 1904
AvaII GGWCC 2 cut(s) 1462, 1901
AvrII CCTAGG 1 cut(s) 421
AxyI CCTNAGG 1 cut(s) 1575
BaeGI GKGCMC 2 cut(s) 1057, 1193
BanI GGYRCC 1 cut(s) 1052
BarI GAAGNNNNNNTAC 2 cut(s) 1146, 1178
Bbv12I GWGCWC 2 cut(s) 1206, 1285
BbvI GCAGC 4 cut(s) 61, 64, 83, 505
BccI CCATC 8 cut(s) 725, 732, 1616, 1643, 1710, 1883, 1889, 1955
BcgI CGANNNNNNTGC 2 cut(s) 1918, 1952
BciT130I CCWGG 6 cut(s) 93, 550, 702, 1187, 1467, 1905
BciVI GTATCC 2 cut(s) 1448, 1463
BclI TGATCA 2 cut(s) 1069, 1414
BcoDI GTCTC 3 cut(s) 896, 1142, 1826
BcuI ACTAGT 2 cut(s) 881, 1165
BfaI CTAG 8 cut(s) 51, 422, 882, 1166, 1286, 1427, 1529, 1772
BfmI CTRYAG 6 cut(s) 675, 750, 921, 1275, 1510, 1809
BfuAI ACCTGC 2 cut(s) 156, 636
BfuI GTATCC 2 cut(s) 1448, 1463
BisI GCNGC 5 cut(s) 72, 75, 78, 112, 519
BlnI CCTAGG 1 cut(s) 421
BlsI GCNGC 5 cut(s) 73, 76, 79, 113, 520
BmcAI AGTACT 2 cut(s) 55, 839
Bme1390I CCNGG 6 cut(s) 93, 550, 702, 1187, 1467, 1905
Bme18I GGWCC 2 cut(s) 1462, 1901
BmgT120I GGNCC 4 cut(s) 659, 1462, 1699, 1901
BmiI GGNNCC 2 cut(s) 1054, 1464
BmrFI CCNGG 6 cut(s) 93, 550, 702, 1187, 1467, 1905
BmsI GCATC 3 cut(s) 1274, 1577, 1804
BoxI GACNNNNGTC 1 cut(s) 1154
BpuEI CTTGAG 3 cut(s) 66, 953, 1086
BsaHI GRCGYC 1 cut(s) 1506
BsaJI CCNNGG 9 cut(s) 421, 739, 970, 1185, 1186, 1465, 1764, 1799, 1866
BsaXI ACNNNNNCTCC 8 cut(s) 530, 560, 578, 608, 882, 912, 1283, 1313
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 668
Bse1I ACTGG 1 cut(s) 1914
Bse21I CCTNAGG 1 cut(s) 1575
Bse3DI GCAATG 1 cut(s) 310
BseBI CCWGG 6 cut(s) 93, 550, 702, 1187, 1467, 1905
BseDI CCNNGG 9 cut(s) 421, 739, 970, 1185, 1186, 1465, 1764, 1799, 1866
BseGI GGATG 5 cut(s) 311, 482, 724, 795, 1795
BseLI CCNNNNNNNGG 2 cut(s) 43, 668
BseMI GCAATG 1 cut(s) 310
BseMII CTCAG 2 cut(s) 114, 1566
BseNI ACTGG 1 cut(s) 1914
BseRI GAGGAG 2 cut(s) 506, 1291
BseSI GKGCMC 2 cut(s) 1057, 1193
BseXI GCAGC 4 cut(s) 61, 64, 83, 505
BshFI GGCC 7 cut(s) 336, 661, 756, 941, 1573, 1700, 1744
BshNI GGYRCC 1 cut(s) 1052
BsiHKAI GWGCWC 2 cut(s) 1206, 1285
BslFI GGGAC 2 cut(s) 813, 1142
BslI CCNNNNNNNGG 2 cut(s) 43, 668
BsmAI GTCTC 3 cut(s) 896, 1142, 1826
BsmFI GGGAC 2 cut(s) 813, 1142
BsnI GGCC 7 cut(s) 336, 661, 756, 941, 1573, 1700, 1744
Bsp1286I GDGCHC 4 cut(s) 1057, 1193, 1206, 1285
Bsp143I GATC 5 cut(s) 893, 1069, 1414, 1813, 1850
Bsp19I CCATGG 1 cut(s) 1764
BspACI CCGC 2 cut(s) 111, 560
BspANI GGCC 7 cut(s) 336, 661, 756, 941, 1573, 1700, 1744
BspCNI CTCAG 2 cut(s) 113, 1567
BspLI GGNNCC 2 cut(s) 1054, 1464
BspMAI CTGCAG 3 cut(s) 925, 1279, 1813
BspMI ACCTGC 2 cut(s) 156, 636
BspPI GGATC 2 cut(s) 901, 1858
BspT107I GGYRCC 1 cut(s) 1052
BsrDI GCAATG 1 cut(s) 310
BsrI ACTGG 1 cut(s) 1914
BssECI CCNNGG 9 cut(s) 421, 739, 970, 1185, 1186, 1465, 1764, 1799, 1866
BssMI GATC 5 cut(s) 893, 1069, 1414, 1813, 1850
BssNAI GTATAC 1 cut(s) 1171
BssNI GRCGYC 1 cut(s) 1506
BssT1I CCWWGG 5 cut(s) 421, 739, 970, 1764, 1866
Bst1107I GTATAC 1 cut(s) 1171
Bst2UI CCWGG 6 cut(s) 93, 550, 702, 1187, 1467, 1905
Bst4CI ACNGT 3 cut(s) 385, 504, 994
Bst6I CTCTTC 1 cut(s) 1824
BstACI GRCGYC 1 cut(s) 1506
BstAPI GCANNNNNTGC 1 cut(s) 77
BstC8I GCNNGC 4 cut(s) 286, 338, 631, 1931
BstDEI CTNAG 2 cut(s) 100, 1575
BstDSI CCRYGG 2 cut(s) 1764, 1799
BstF5I GGATG 5 cut(s) 311, 482, 724, 795, 1795
BstHHI GCGC 1 cut(s) 653
BstKTI GATC 5 cut(s) 896, 1072, 1417, 1816, 1853
BstMAI GTCTC 3 cut(s) 896, 1142, 1826
BstMBI GATC 5 cut(s) 893, 1069, 1414, 1813, 1850
BstMWI GCNNNNNNNGC 5 cut(s) 77, 83, 337, 635, 1706
BstNI CCWGG 6 cut(s) 93, 550, 702, 1187, 1467, 1905
BstNSI RCATGY 1 cut(s) 1933
BstPAI GACNNNNGTC 1 cut(s) 1154
BstSCI CCNGG 6 cut(s) 91, 548, 700, 1185, 1465, 1903
BstSFI CTRYAG 6 cut(s) 675, 750, 921, 1275, 1510, 1809
BstSLI GKGCMC 2 cut(s) 1057, 1193
BstV1I GCAGC 4 cut(s) 61, 64, 83, 505
BstX2I RGATCY 1 cut(s) 893
BstXI CCANNNNNNTGG 2 cut(s) 274, 731
BstYI RGATCY 1 cut(s) 893
BstZ17I GTATAC 1 cut(s) 1171
Bsu36I CCTNAGG 1 cut(s) 1575
BsuI GTATCC 2 cut(s) 1448, 1463
BsuRI GGCC 7 cut(s) 336, 661, 756, 941, 1573, 1700, 1744
BtgI CCRYGG 2 cut(s) 1764, 1799
BtsCI GGATG 5 cut(s) 311, 482, 724, 795, 1795
BtsIMutI CAGTG 1 cut(s) 1921
BveI ACCTGC 2 cut(s) 156, 636
Cac8I GCNNGC 4 cut(s) 286, 338, 631, 1931
CaiI CAGNNNCTG 3 cut(s) 74, 77, 1274
CfoI GCGC 1 cut(s) 653
Cfr13I GGNCC 4 cut(s) 659, 1462, 1699, 1901
CsiI ACCWGGT 1 cut(s) 700
Csp6I GTAC 3 cut(s) 54, 838, 1908
CspCI CAANNNNNGTGG 2 cut(s) 1533, 1568
CviAII CATG 4 cut(s) 956, 1750, 1765, 1930
CviQI GTAC 3 cut(s) 54, 838, 1908
DdeI CTNAG 2 cut(s) 100, 1575
DpnI GATC 5 cut(s) 895, 1071, 1416, 1815, 1852
DpnII GATC 5 cut(s) 893, 1069, 1414, 1813, 1850
DraI TTTAAA 1 cut(s) 528
Eam1104I CTCTTC 1 cut(s) 1824
EarI CTCTTC 1 cut(s) 1824
Eco130I CCWWGG 5 cut(s) 421, 739, 970, 1764, 1866
Eco147I AGGCCT 3 cut(s) 756, 1573, 1744
Eco32I GATATC 1 cut(s) 357
Eco47I GGWCC 2 cut(s) 1462, 1901
Eco57I CTGAAG 2 cut(s) 300, 824
Eco81I CCTNAGG 1 cut(s) 1575
EcoRI GAATTC 1 cut(s) 158
EcoRII CCWGG 6 cut(s) 91, 548, 700, 1185, 1465, 1903
EcoRV GATATC 1 cut(s) 357
EcoT14I CCWWGG 5 cut(s) 421, 739, 970, 1764, 1866
ErhI CCWWGG 5 cut(s) 421, 739, 970, 1764, 1866
FaeI CATG 4 cut(s) 959, 1753, 1768, 1933
FaqI GGGAC 2 cut(s) 813, 1142
FatI CATG 4 cut(s) 955, 1749, 1764, 1929
FauNDI CATATG 2 cut(s) 458, 1954
FbaI TGATCA 2 cut(s) 1069, 1414
FblI GTMKAC 1 cut(s) 1170
Fnu4HI GCNGC 5 cut(s) 72, 75, 78, 112, 519
FokI GGATG 5 cut(s) 298, 469, 711, 802, 1782
Fsp4HI GCNGC 5 cut(s) 72, 75, 78, 112, 519
FspBI CTAG 8 cut(s) 51, 422, 882, 1166, 1286, 1427, 1529, 1772
GlaI GCGC 1 cut(s) 652
GluI GCNGC 5 cut(s) 72, 75, 78, 112, 519
HaeIII GGCC 7 cut(s) 336, 661, 756, 941, 1573, 1700, 1744
HhaI GCGC 1 cut(s) 653
Hin1I GRCGYC 1 cut(s) 1506
Hin1II CATG 4 cut(s) 959, 1753, 1768, 1933
Hin6I GCGC 1 cut(s) 651
HinP1I GCGC 1 cut(s) 651
HincII GTYRAC 1 cut(s) 1108
HindII GTYRAC 1 cut(s) 1108
HindIII AAGCTT 1 cut(s) 282
HinfI GANTC 5 cut(s) 12, 210, 593, 966, 1060
HphI GGTGA 6 cut(s) 263, 986, 1099, 1174, 1845, 1904
Hpy166II GTNNAC 3 cut(s) 997, 1108, 1171
Hpy188I TCNGA 6 cut(s) 103, 361, 1253, 1270, 1384, 1850
Hpy188III TCNNGA 1 cut(s) 728
Hpy8I GTNNAC 3 cut(s) 997, 1108, 1171
HpyAV CCTTC 8 cut(s) 242, 353, 613, 704, 931, 971, 1658, 1769
HpyCH4III ACNGT 3 cut(s) 385, 504, 994
HpyCH4IV ACGT 3 cut(s) 273, 1399, 1506
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 83, 337, 635, 1706
HpyF3I CTNAG 2 cut(s) 100, 1575
HpySE526I ACGT 3 cut(s) 273, 1399, 1506
Hsp92I GRCGYC 1 cut(s) 1506
Hsp92II CATG 4 cut(s) 959, 1753, 1768, 1933
HspAI GCGC 1 cut(s) 651
Ksp22I TGATCA 2 cut(s) 1069, 1414
Kzo9I GATC 5 cut(s) 893, 1069, 1414, 1813, 1850
LmnI GCTCC 4 cut(s) 493, 1280, 1391, 1768
Lsp1109I GCAGC 4 cut(s) 61, 64, 83, 505
LweI GCATC 3 cut(s) 1274, 1577, 1804
MabI ACCWGGT 1 cut(s) 700
MaeI CTAG 8 cut(s) 51, 422, 882, 1166, 1286, 1427, 1529, 1772
MaeII ACGT 3 cut(s) 273, 1399, 1506
MaeIII GTNAC 8 cut(s) 143, 226, 307, 392, 680, 1502, 1625, 1922
MalI GATC 5 cut(s) 895, 1071, 1416, 1815, 1852
MboI GATC 5 cut(s) 893, 1069, 1414, 1813, 1850
MboII GAAGA 7 cut(s) 167, 440, 817, 938, 1270, 1631, 1841
MfeI CAATTG 1 cut(s) 169
MflI RGATCY 1 cut(s) 893
MhlI GDGCHC 4 cut(s) 1057, 1193, 1206, 1285
MlyI GAGTC 4 cut(s) 6, 219, 587, 1054
MmeI TCCRAC 2 cut(s) 291, 757
MroXI GAANNNNTTC 1 cut(s) 623
MseI TTAA 7 cut(s) 206, 527, 567, 866, 1080, 1659, 1946
MslI CAYNNNNRTG 4 cut(s) 729, 1116, 1240, 1329
MspA1I CMGCKG 2 cut(s) 74, 560
MspR9I CCNGG 6 cut(s) 93, 550, 702, 1187, 1467, 1905
MunI CAATTG 1 cut(s) 169
MvaI CCWGG 6 cut(s) 93, 550, 702, 1187, 1467, 1905
MwoI GCNNNNNNNGC 5 cut(s) 77, 83, 337, 635, 1706
NcoI CCATGG 1 cut(s) 1764
NdeI CATATG 2 cut(s) 458, 1954
NdeII GATC 5 cut(s) 893, 1069, 1414, 1813, 1850
NlaIII CATG 4 cut(s) 959, 1753, 1768, 1933
NlaIV GGNNCC 2 cut(s) 1054, 1464
NmeAIII GCCGAG 2 cut(s) 312, 494
NmuCI GTSAC 5 cut(s) 307, 392, 1502, 1625, 1922
NspI RCATGY 1 cut(s) 1933
OliI CACNNNNGTG 1 cut(s) 1240
PaeI GCATGC 1 cut(s) 1933
PaqCI CACCTGC 1 cut(s) 636
PasI CCCWGGG 1 cut(s) 1186
PceI AGGCCT 3 cut(s) 756, 1573, 1744
PdmI GAANNNNTTC 1 cut(s) 623
PfeI GAWTC 1 cut(s) 966
PkrI GCNGC 5 cut(s) 73, 76, 79, 113, 520
PleI GAGTC 4 cut(s) 6, 218, 587, 1054
PpsI GAGTC 4 cut(s) 6, 218, 587, 1054
PshAI GACNNNNGTC 1 cut(s) 1154
PshBI ATTAAT 1 cut(s) 1659
Psp6I CCWGG 6 cut(s) 91, 548, 700, 1185, 1465, 1903
PspGI CCWGG 6 cut(s) 91, 548, 700, 1185, 1465, 1903
PspN4I GGNNCC 2 cut(s) 1054, 1464
PspPI GGNCC 4 cut(s) 659, 1462, 1699, 1901
PstI CTGCAG 3 cut(s) 925, 1279, 1813
PstNI CAGNNNCTG 3 cut(s) 74, 77, 1274
PsuI RGATCY 1 cut(s) 893
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 3 cut(s) 55, 839, 1909
RsaNI GTAC 3 cut(s) 54, 838, 1908
RseI CAYNNNNRTG 4 cut(s) 729, 1116, 1240, 1329
SaqAI TTAA 7 cut(s) 206, 527, 567, 866, 1080, 1659, 1946
SatI GCNGC 5 cut(s) 72, 75, 78, 112, 519
Sau3AI GATC 5 cut(s) 893, 1069, 1414, 1813, 1850
Sau96I GGNCC 4 cut(s) 659, 1462, 1699, 1901
ScaI AGTACT 2 cut(s) 55, 839
SchI GAGTC 4 cut(s) 6, 219, 587, 1054
ScrFI CCNGG 6 cut(s) 93, 550, 702, 1187, 1467, 1905
SduI GDGCHC 4 cut(s) 1057, 1193, 1206, 1285
SexAI ACCWGGT 1 cut(s) 700
SfaNI GCATC 3 cut(s) 1274, 1577, 1804
SfcI CTRYAG 6 cut(s) 675, 750, 921, 1275, 1510, 1809
SinI GGWCC 2 cut(s) 1462, 1901
SmiMI CAYNNNNRTG 4 cut(s) 729, 1116, 1240, 1329
SmlI CTYRAG 3 cut(s) 81, 932, 1101
SmoI CTYRAG 3 cut(s) 81, 932, 1101
SpeI ACTAGT 2 cut(s) 881, 1165
SphI GCATGC 1 cut(s) 1933
SseBI AGGCCT 3 cut(s) 756, 1573, 1744
SsiI CCGC 2 cut(s) 111, 560
SspMI CTAG 8 cut(s) 51, 422, 882, 1166, 1286, 1427, 1529, 1772
StuI AGGCCT 3 cut(s) 756, 1573, 1744
StyD4I CCNGG 6 cut(s) 91, 548, 700, 1185, 1465, 1903
StyI CCWWGG 5 cut(s) 421, 739, 970, 1764, 1866
TaaI ACNGT 3 cut(s) 385, 504, 994
TaiI ACGT 3 cut(s) 276, 1402, 1509
TaqI TCGA 2 cut(s) 438, 1938
TatI WGTACW 2 cut(s) 53, 837
TauI GCSGC 1 cut(s) 114
TfiI GAWTC 1 cut(s) 966
Tru1I TTAA 7 cut(s) 206, 527, 567, 866, 1080, 1659, 1946
Tru9I TTAA 7 cut(s) 206, 527, 567, 866, 1080, 1659, 1946
TscAI CASTG 1 cut(s) 1921
TseFI GTSAC 5 cut(s) 307, 392, 1502, 1625, 1922
TseI GCWGC 4 cut(s) 71, 74, 77, 518
Tsp45I GTSAC 5 cut(s) 307, 392, 1502, 1625, 1922
TspDTI ATGAA 9 cut(s) 261, 618, 723, 808, 972, 1168, 1358, 1393, 1677
TspRI CASTG 1 cut(s) 1921
VpaK11BI GGWCC 2 cut(s) 1462, 1901
VspI ATTAAT 1 cut(s) 1659
XapI RAATTY 4 cut(s) 158, 744, 1638, 1882
XceI RCATGY 1 cut(s) 1933
XcmI CCANNNNNNNNNTGG 1 cut(s) 698
XmaJI CCTAGG 1 cut(s) 421
XmiI GTMKAC 1 cut(s) 1170
XmnI GAANNNNTTC 1 cut(s) 623
XspI CTAG 8 cut(s) 51, 422, 882, 1166, 1286, 1427, 1529, 1772
ZraI GACGTC 1 cut(s) 1507
ZrmI AGTACT 2 cut(s) 55, 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.