RLG00000029324

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000066
Physical Location & Seq
Reverse (-)
36537118 .. 36539836
2719 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029324

Sequence Viewer

Length: 2364 bp
ATGGCATGCTTACATGGGAGAATGCTTCTTTTGCAACTCTCTTTGGCCGCAGTGCTATTAGCCTATGCAGCAACAACAATGCTAGCAGCTGCTCAAGCCCCGCCTGAATGCAATGAAAGCTGTGGTAGTGTCTCAATTCCATATCCGTTTGGTTTGAGTGATGGTTGTTACCTATATGTGCCTGGACAAGATGCTCAGCCATTCAAGATCACTTGCGACAACAGCACCTCGCAACCATCCCTAAAGTTCTTGGATAGCTATTACTTTCCCACAAACATTACCAACATTTCTGTGGGTGAAAGTGAGTTGCAAGTAATGGTGACCACGTCCCGAAACTGTTGGCCCAACAATTCATACGACTCCTCCTTGTACAGAGGCAGTGTGCTCGATCTGCCTTCATCTTACACCATCTCCGACAAAAACAAGGTTTTTTCCGTTGGGTGCAACAAAGTATCGATGTTCAAAGGTTTTCCTCTTAAAGTCACAGATCCAGAGCAACCCTTTGCAGTAGGGTTCAGCGGTGTAAGCCTGTGTCAAGATGAATTTGGCAAAAGATTCCCTGAGACTTGCACCGGATTTGGGTGCTCCGGAAATCCTATCCCTAGTGGACTGCAAAATATTACGGTGGATGTGTGGACACTAGGTACCACCCTTGGAAAAACGGAGCAGTGGGCCCTGAGCTACCCTTGCAGCTACGGCTTCGTTGTGGATGAGCGCAATTTCACATTCGCCGGCAATCAAAGTTTTAATGAGCTGAACGGCAGAAGACAGGAGCTTCCGGTGCTTGCTAATTGGGCGATTGGGAGTGACAACTGCGAAGCAGCAAAGAAGAACAATGAGACCGCTTTTGCATGCAAGGGTGTTAATACGAAGTGTGTCGACCGCGCTGGTGGTTACTTTTGCCAGTGTGAGACAGGCTACGCAGGGAACCCGTACCTCCTCAATGATTGCCTAGATATCAATGAGTGCAATAATTTAACCCTCTGTAGTGCTCATGCAACTTGCATAAACTCAATTGGAAGTTACACTTGTAAATGTGACAAGGGCTATAGAAACGATGACAACGACAAGAATACTTGTGTCCAAATCAACGAAACCTTCAGCAAAAATGACAAAGAAATGCAAATTTCACTGGGTGTCTGTTTAAGCTTCCTAGTCGTGTTGATTATAACATTTTGGATATACTGCGGAATGAAAAGAAGAAAGTTCAAGCAACTCAAAGAAAAGTACTTCAAAGACAATGGTGGCGTCTTGTTACAACAAAAATTGAAGAATTTTGACGGACCTCAAGCAGCCAAAATCTTTACAAGAGAAGAGCTTAAGAAGGCCACAAACAACTTTGATGACAGCAGAAAAATTGGTGAAGGAGGCTATGGATTTGTTTATAGAGGAACATTGCCAGATAAAAAGGAGGTTGCCATAAAAGTGTCAAAAAGTAATGCCCCAATGACCCAAAGTAATCAATTCATTAATGAGGTAATTGTTCTTTCTCAAATCAACCATAGAAATGTCGTGAGGCTCCTAGGTTGTTGTTTGGAAACCCAAACACCTTTTCTGGTTTACGAGTTTATTAGCAATGGCACTCTTTATGAGCACATTCATAAAAAGAATGGCAAGGGACCGCTTTCTTTCGGGTTACGAATGAAGATCGCAGCAGAAACAGCAGGATCCCTAGCATACTTGCACTACTCTACTTCAATGCAGATTGTGCACCGAGATGTGAAAGCAACGAATATACTTTTGGATGACAATTTCACAGCCAAAGTGTCAGATTTTGGAGCTTCGAAATTGGTTCCTGAAGATCAAAATCAGCTGTCCACCTTAGTGCAAGGTACGCTTGGGTACCTAGACCCTGAATATCTCCAGTCAAACACACTGACAGAGAAGAGTGATGTCTATAGTTTCGGCGTTGTCCTTGTGGAGCTAATAACGAGTCAAGTGGCAATTTCTTACAAGAAGCCTGAGGCAGAGAGAAACCTAGCAAACTTTTTTGTAACGTCGGTGGAAGAGAATCGCTTGGATCAGATTCTTGATCTTGAAATAATCAAAGAGGGAAGCTTTGAAATAGCTGAGCAAGTTGCCCATCTCGCCAAAAGATGTTTAAGTTTGAAAGGGGGGGATAGGCCTACGATGAAAGAAGTAGCTATGGAGCTAGAGGGAATACTGCAAGTTCTGGCAAAGCATCCAGAGGGAAAGCCTGATGCCTCTCCGAAAGAGACCGACTACTTGCTTGCGATGTCACCTGCAAATGCTTACGTTGTTGATGTTAGAGGTGATGAAGGTGAAGTTATAACCAGTATTGACTACGACCAGAGCATGCAGAATCAAGCCCAGATAATGAAGCCTGATGATGGTGGGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

788

Amino Acids

86.96

Weight (kDa)

5.43

Isoelectric Point (pI)

31.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 37 - 105 8.7e-10 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 319 - 357 4.7e-09 Calcium-binding EGF domain
Pkinase PF00069 448 - 714 4.5e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 450 - 718 1.4e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1169, 2291
AarI CACCTGC 1 cut(s) 2251
Acc36I ACCTGC 1 cut(s) 2251
Acc65I GGTACC 2 cut(s) 644, 1842
AccB1I GGYRCC 2 cut(s) 644, 1842
AccI GTMKAC 1 cut(s) 879
AccII CGCG 1 cut(s) 885
AccIII TCCGGA 1 cut(s) 587
AciI CCGC 7 cut(s) 48, 101, 519, 843, 883, 1188, 1622
AclWI GGATC 4 cut(s) 482, 1662, 1675, 2028
AcoI YGGCCR 1 cut(s) 45
AcsI RAATTY 3 cut(s) 542, 1125, 1273
AcuI CTGAAG 2 cut(s) 1084, 1818
AcyI GRCGYC 1 cut(s) 1248
AdeI CACNNNGTG 1 cut(s) 1136
AfaI GTAC 6 cut(s) 371, 646, 935, 1229, 1834, 1844
AfiI CCNNNNNNNGG 2 cut(s) 579, 2351
AflII CTTAAG 1 cut(s) 1319
AgsI TTSAA 9 cut(s) 205, 463, 1210, 1234, 1270, 1698, 2039, 2063, 2110
AjiI CACGTC 1 cut(s) 327
AjnI CCWGG 1 cut(s) 181
AjuI GAANNNNNNNTTGG 4 cut(s) 1536, 1568, 1724, 1756
AleI CACNNNNGTG 1 cut(s) 1823
Alw21I GWGCWC 5 cut(s) 387, 587, 994, 1596, 1713
Alw26I GTCTC 5 cut(s) 136, 557, 833, 905, 2210
Alw44I GTGCAC 1 cut(s) 1709
AlwI GGATC 4 cut(s) 482, 1662, 1675, 2028
Aor13HI TCCGGA 1 cut(s) 587
AoxI GGCC 5 cut(s) 45, 341, 672, 1326, 2123
ApaI GGGCCC 1 cut(s) 676
ApaLI GTGCAC 1 cut(s) 1709
ApeKI GCWGC 7 cut(s) 68, 86, 89, 690, 821, 1292, 1652
ApoI RAATTY 3 cut(s) 542, 1125, 1273
AseI ATTAAT 1 cut(s) 1470
Asp718I GGTACC 2 cut(s) 644, 1842
AspA2I CCTAGG 1 cut(s) 1522
AspLEI GCGC 2 cut(s) 717, 887
AspS9I GGNCC 5 cut(s) 342, 672, 673, 1283, 1619
AsuHPI GGTGA 6 cut(s) 308, 331, 1373, 2232, 2285, 2294
AsuII TTCGAA 1 cut(s) 1784
AsuNHI GCTAGC 1 cut(s) 82
AvaII GGWCC 2 cut(s) 1283, 1619
AvrII CCTAGG 1 cut(s) 1522
AxyI CCTNAGG 1 cut(s) 1962
BaeGI GKGCMC 2 cut(s) 676, 1713
BamHI GGATCC 1 cut(s) 1667
BanI GGYRCC 2 cut(s) 644, 1842
BanII GRGCYC 1 cut(s) 676
BbsI GAAGAC 1 cut(s) 772
Bbv12I GWGCWC 5 cut(s) 387, 587, 994, 1596, 1713
BbvI GCAGC 7 cut(s) 76, 80, 98, 702, 833, 1304, 1664
BccI CCATC 5 cut(s) 155, 244, 416, 2091, 2345
BceAI ACGGC 2 cut(s) 712, 775
BcgI CGANNNNNNTGC 2 cut(s) 367, 401
BciT130I CCWGG 1 cut(s) 183
BcoDI GTCTC 5 cut(s) 136, 557, 833, 905, 2210
BfmI CTRYAG 3 cut(s) 985, 1048, 1897
BfrI CTTAAG 1 cut(s) 1319
BfuAI ACCTGC 1 cut(s) 2251
BisI GCNGC 8 cut(s) 48, 69, 87, 90, 691, 822, 1293, 1653
BlnI CCTAGG 1 cut(s) 1522
BlpI GCTNAGC 2 cut(s) 195, 2070
BlsI GCNGC 8 cut(s) 49, 70, 88, 91, 692, 823, 1294, 1654
BmcAI AGTACT 1 cut(s) 1229
Bme1390I CCNGG 1 cut(s) 183
Bme18I GGWCC 2 cut(s) 1283, 1619
BmgBI CACGTC 1 cut(s) 327
BmgT120I GGNCC 5 cut(s) 342, 672, 673, 1283, 1619
BmiI GGNNCC 8 cut(s) 646, 674, 929, 1520, 1620, 1669, 1794, 1844
BmrFI CCNGG 1 cut(s) 183
BmrI ACTGGG 1 cut(s) 1142
BmsI GCATC 3 cut(s) 181, 2191, 2191
BmtI GCTAGC 1 cut(s) 86
BmuI ACTGGG 1 cut(s) 1142
BpiI GAAGAC 1 cut(s) 772
BpmI CTGGAG 1 cut(s) 1847
Bpu10I CCTNAGC 1 cut(s) 677
Bpu1102I GCTNAGC 2 cut(s) 195, 2070
Bpu14I TTCGAA 1 cut(s) 1784
BpuEI CTTGAG 2 cut(s) 78, 1272
Bsa29I ATCGAT 1 cut(s) 455
BsaBI GATNNNNATC 1 cut(s) 1806
BsaHI GRCGYC 1 cut(s) 1248
BsaI GGTCTC 2 cut(s) 833, 2210
BsaJI CCNNGG 2 cut(s) 652, 1522
BsaWI WCCGGW 3 cut(s) 572, 587, 778
BsaXI ACNNNNNCTCC 4 cut(s) 347, 377, 395, 425
Bsc4I CCNNNNNNNGG 2 cut(s) 579, 2351
Bse118I RCCGGY 1 cut(s) 731
Bse1I ACTGG 4 cut(s) 904, 1137, 1864, 2295
Bse21I CCTNAGG 1 cut(s) 1962
Bse3DI GCAATG 3 cut(s) 118, 1394, 1582
Bse8I GATNNNNATC 1 cut(s) 1806
BseAI TCCGGA 1 cut(s) 587
BseBI CCWGG 1 cut(s) 183
BseCI ATCGAT 1 cut(s) 455
BseDI CCNNGG 2 cut(s) 652, 1522
BseGI GGATG 5 cut(s) 236, 634, 715, 1750, 2182
BseJI GATNNNNATC 1 cut(s) 1806
BseLI CCNNNNNNNGG 2 cut(s) 579, 2351
BseMI GCAATG 3 cut(s) 118, 1394, 1582
BseMII CTCAG 5 cut(s) 209, 552, 668, 1953, 2061
BseNI ACTGG 4 cut(s) 904, 1137, 1864, 2295
BseRI GAGGAG 2 cut(s) 352, 929
BseSI GKGCMC 2 cut(s) 676, 1713
BseXI GCAGC 7 cut(s) 76, 80, 98, 702, 833, 1304, 1664
Bsh1236I CGCG 1 cut(s) 885
Bsh1285I CGRYCG 1 cut(s) 883
BshFI GGCC 5 cut(s) 47, 343, 674, 1328, 2125
BshNI GGYRCC 2 cut(s) 644, 1842
BshVI ATCGAT 1 cut(s) 455
BsiEI CGRYCG 1 cut(s) 883
BsiHKAI GWGCWC 5 cut(s) 387, 587, 994, 1596, 1713
BsiSI CCGG 4 cut(s) 573, 588, 732, 779
BslFI GGGAC 2 cut(s) 313, 1632
BslI CCNNNNNNNGG 2 cut(s) 579, 2351
BsmAI GTCTC 5 cut(s) 136, 557, 833, 905, 2210
BsmFI GGGAC 2 cut(s) 313, 1632
BsmI GAATGC 2 cut(s) 27, 113
BsnI GGCC 5 cut(s) 47, 343, 674, 1328, 2125
Bso31I GGTCTC 2 cut(s) 833, 2210
Bsp119I TTCGAA 1 cut(s) 1784
Bsp120I GGGCCC 1 cut(s) 672
Bsp1286I GDGCHC 6 cut(s) 387, 587, 676, 994, 1596, 1713
Bsp13I TCCGGA 1 cut(s) 587
Bsp1407I TGTACA 1 cut(s) 369
Bsp143I GATC 8 cut(s) 207, 388, 487, 1647, 1667, 1801, 2020, 2032
Bsp1720I GCTNAGC 2 cut(s) 195, 2070
BspACI CCGC 7 cut(s) 48, 101, 519, 843, 883, 1188, 1622
BspANI GGCC 5 cut(s) 47, 343, 674, 1328, 2125
BspCNI CTCAG 5 cut(s) 208, 553, 669, 1954, 2062
BspDI ATCGAT 1 cut(s) 455
BspEI TCCGGA 1 cut(s) 587
BspFNI CGCG 1 cut(s) 885
BspLI GGNNCC 8 cut(s) 646, 674, 929, 1520, 1620, 1669, 1794, 1844
BspMI ACCTGC 1 cut(s) 2251
BspOI GCTAGC 1 cut(s) 86
BspPI GGATC 4 cut(s) 482, 1662, 1675, 2028
BspQI GCTCTTC 1 cut(s) 1308
BspT104I TTCGAA 1 cut(s) 1784
BspT107I GGYRCC 2 cut(s) 644, 1842
BspTI CTTAAG 1 cut(s) 1319
BspTNI GGTCTC 2 cut(s) 833, 2210
BsrDI GCAATG 3 cut(s) 118, 1394, 1582
BsrFI RCCGGY 1 cut(s) 731
BsrGI TGTACA 1 cut(s) 369
BsrI ACTGG 4 cut(s) 904, 1137, 1864, 2295
BssAI RCCGGY 1 cut(s) 731
BssECI CCNNGG 2 cut(s) 652, 1522
BssMI GATC 8 cut(s) 207, 388, 487, 1647, 1667, 1801, 2020, 2032
BssNI GRCGYC 1 cut(s) 1248
BssT1I CCWWGG 2 cut(s) 652, 1522
Bst2UI CCWGG 1 cut(s) 183
Bst4CI ACNGT 2 cut(s) 338, 625
Bst6I CTCTTC 3 cut(s) 1308, 1880, 2001
BstACI GRCGYC 1 cut(s) 1248
BstAFI CTTAAG 1 cut(s) 1319
BstAPI GCANNNNNTGC 1 cut(s) 1708
BstAUI TGTACA 1 cut(s) 369
BstBI TTCGAA 1 cut(s) 1784
BstC8I GCNNGC 7 cut(s) 7, 84, 733, 786, 853, 2232, 2318
BstDEI CTNAG 6 cut(s) 195, 561, 677, 1822, 1962, 2070
BstEII GGTNACC 1 cut(s) 319
BstF5I GGATG 5 cut(s) 236, 634, 715, 1750, 2182
BstFNI CGCG 1 cut(s) 885
BstHHI GCGC 2 cut(s) 717, 887
BstKTI GATC 8 cut(s) 210, 391, 490, 1650, 1670, 1804, 2023, 2035
BstMAI GTCTC 5 cut(s) 136, 557, 833, 905, 2210
BstMBI GATC 8 cut(s) 207, 388, 487, 1647, 1667, 1801, 2020, 2032
BstMCI CGRYCG 1 cut(s) 883
BstNI CCWGG 1 cut(s) 183
BstNSI RCATGY 3 cut(s) 9, 855, 2320
BstPI GGTNACC 1 cut(s) 319
BstSCI CCNGG 1 cut(s) 181
BstSFI CTRYAG 3 cut(s) 985, 1048, 1897
BstSLI GKGCMC 2 cut(s) 676, 1713
BstUI CGCG 1 cut(s) 885
BstV1I GCAGC 7 cut(s) 76, 80, 98, 702, 833, 1304, 1664
BstV2I GAAGAC 1 cut(s) 772
BstX2I RGATCY 2 cut(s) 487, 1667
BstYI RGATCY 2 cut(s) 487, 1667
Bsu15I ATCGAT 1 cut(s) 455
Bsu36I CCTNAGG 1 cut(s) 1962
BsuRI GGCC 5 cut(s) 47, 343, 674, 1328, 2125
BsuTUI ATCGAT 1 cut(s) 455
BtgZI GCGATG 1 cut(s) 2249
BtrI CACGTC 1 cut(s) 327
BtsCI GGATG 5 cut(s) 236, 634, 715, 1750, 2182
BtsI GCAGTG 3 cut(s) 57, 385, 674
BtsIMutI CAGTG 6 cut(s) 57, 385, 674, 911, 1130, 1874
BveI ACCTGC 1 cut(s) 2251
Cac8I GCNNGC 7 cut(s) 7, 84, 733, 786, 853, 2232, 2318
CfoI GCGC 2 cut(s) 717, 887
Cfr10I RCCGGY 1 cut(s) 731
Cfr13I GGNCC 5 cut(s) 342, 672, 673, 1283, 1619
ClaI ATCGAT 1 cut(s) 455
CseI GACGC 1 cut(s) 1237
Csp6I GTAC 6 cut(s) 370, 645, 934, 1228, 1833, 1843
CviAII CATG 5 cut(s) 6, 14, 852, 995, 2317
CviQI GTAC 6 cut(s) 370, 645, 934, 1228, 1833, 1843
DdeI CTNAG 6 cut(s) 195, 561, 677, 1822, 1962, 2070
DpnI GATC 8 cut(s) 209, 390, 489, 1649, 1669, 1803, 2022, 2034
DpnII GATC 8 cut(s) 207, 388, 487, 1647, 1667, 1801, 2020, 2032
DraIII CACNNNGTG 1 cut(s) 1136
EaeI YGGCCR 1 cut(s) 45
Eam1104I CTCTTC 3 cut(s) 1308, 1880, 2001
EarI CTCTTC 3 cut(s) 1308, 1880, 2001
Eco130I CCWWGG 2 cut(s) 652, 1522
Eco147I AGGCCT 1 cut(s) 2125
Eco24I GRGCYC 1 cut(s) 676
Eco31I GGTCTC 2 cut(s) 833, 2210
Eco32I GATATC 1 cut(s) 958
Eco47I GGWCC 2 cut(s) 1283, 1619
Eco57I CTGAAG 2 cut(s) 1084, 1818
Eco81I CCTNAGG 1 cut(s) 1962
Eco91I GGTNACC 1 cut(s) 319
EcoO109I RGGNCCY 1 cut(s) 673
EcoO65I GGTNACC 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 181
EcoRV GATATC 1 cut(s) 958
EcoT14I CCWWGG 2 cut(s) 652, 1522
EcoT38I GRGCYC 1 cut(s) 676
ErhI CCWWGG 2 cut(s) 652, 1522
FaeI CATG 5 cut(s) 9, 17, 855, 998, 2320
FalI AAGNNNNNCTT 4 cut(s) 1012, 1044, 1821, 1853
FaqI GGGAC 2 cut(s) 313, 1632
FatI CATG 5 cut(s) 5, 13, 851, 994, 2316
FauI CCCGC 1 cut(s) 108
FblI GTMKAC 1 cut(s) 879
Fnu4HI GCNGC 8 cut(s) 48, 69, 87, 90, 691, 822, 1293, 1653
FokI GGATG 5 cut(s) 223, 641, 722, 1757, 2169
FriOI GRGCYC 1 cut(s) 676
Fsp4HI GCNGC 8 cut(s) 48, 69, 87, 90, 691, 822, 1293, 1653
GlaI GCGC 2 cut(s) 716, 886
GluI GCNGC 8 cut(s) 48, 69, 87, 90, 691, 822, 1293, 1653
GsuI CTGGAG 1 cut(s) 1847
HaeIII GGCC 5 cut(s) 47, 343, 674, 1328, 2125
HapII CCGG 4 cut(s) 573, 588, 732, 779
HgaI GACGC 1 cut(s) 1237
HhaI GCGC 2 cut(s) 717, 887
Hin1I GRCGYC 1 cut(s) 1248
Hin1II CATG 5 cut(s) 9, 17, 855, 998, 2320
Hin6I GCGC 2 cut(s) 715, 885
HinP1I GCGC 2 cut(s) 715, 885
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HindIII AAGCTT 2 cut(s) 1147, 2056
HinfI GANTC 6 cut(s) 359, 555, 1933, 2011, 2026, 2323
HpaII CCGG 4 cut(s) 573, 588, 732, 779
HphI GGTGA 6 cut(s) 308, 331, 1373, 2232, 2285, 2294
Hpy166II GTNNAC 6 cut(s) 608, 636, 880, 1561, 1711, 1818
Hpy188I TCNGA 4 cut(s) 415, 1771, 2025, 2211
Hpy8I GTNNAC 6 cut(s) 608, 636, 880, 1561, 1711, 1818
Hpy99I CGWCG 1 cut(s) 2002
HpyAV CCTTC 5 cut(s) 405, 1108, 1318, 1358, 2273
HpyCH4III ACNGT 2 cut(s) 338, 625
HpyCH4IV ACGT 3 cut(s) 326, 1997, 2256
HpyF3I CTNAG 6 cut(s) 195, 561, 677, 1822, 1962, 2070
HpySE526I ACGT 3 cut(s) 326, 1997, 2256
Hsp92I GRCGYC 1 cut(s) 1248
Hsp92II CATG 5 cut(s) 9, 17, 855, 998, 2320
HspAI GCGC 2 cut(s) 715, 885
Kpn2I TCCGGA 1 cut(s) 587
KpnI GGTACC 2 cut(s) 648, 1846
KroI GCCGGC 1 cut(s) 731
KroNI GCCGGC 1 cut(s) 733
Kzo9I GATC 8 cut(s) 207, 388, 487, 1647, 1667, 1801, 2020, 2032
LguI GCTCTTC 1 cut(s) 1308
LmnI GCTCC 7 cut(s) 590, 664, 772, 1524, 1778, 1921, 2149
Lsp1109I GCAGC 7 cut(s) 76, 80, 98, 702, 833, 1304, 1664
LweI GCATC 3 cut(s) 181, 2191, 2191
MaeII ACGT 3 cut(s) 326, 1997, 2256
MalI GATC 8 cut(s) 209, 390, 489, 1649, 1669, 1803, 2022, 2034
MboI GATC 8 cut(s) 207, 388, 487, 1647, 1667, 1801, 2020, 2032
MboII GAAGA 9 cut(s) 777, 841, 1212, 1282, 1325, 1657, 1811, 1897, 2018
MfeI CAATTG 1 cut(s) 1014
MflI RGATCY 2 cut(s) 487, 1667
MhlI GDGCHC 6 cut(s) 387, 587, 676, 994, 1596, 1713
MlyI GAGTC 2 cut(s) 353, 1942
MmeI TCCRAC 1 cut(s) 438
MroI TCCGGA 1 cut(s) 587
MroNI GCCGGC 1 cut(s) 731
MseI TTAA 8 cut(s) 477, 747, 864, 977, 1145, 1320, 1470, 2102
MslI CAYNNNNRTG 5 cut(s) 290, 1424, 1506, 1716, 1823
MspA1I CMGCKG 3 cut(s) 89, 519, 1813
MspCI CTTAAG 1 cut(s) 1319
MspI CCGG 4 cut(s) 573, 588, 732, 779
MspR9I CCNGG 1 cut(s) 183
MunI CAATTG 1 cut(s) 1014
Mva1269I GAATGC 2 cut(s) 27, 113
MvaI CCWGG 1 cut(s) 183
MvnI CGCG 1 cut(s) 885
NaeI GCCGGC 1 cut(s) 733
NdeII GATC 8 cut(s) 207, 388, 487, 1647, 1667, 1801, 2020, 2032
NgoMIV GCCGGC 1 cut(s) 731
NheI GCTAGC 1 cut(s) 82
NlaIII CATG 5 cut(s) 9, 17, 855, 998, 2320
NlaIV GGNNCC 8 cut(s) 646, 674, 929, 1520, 1620, 1669, 1794, 1844
NmuCI GTSAC 5 cut(s) 319, 481, 806, 1037, 2238
NspI RCATGY 3 cut(s) 9, 855, 2320
NspV TTCGAA 1 cut(s) 1784
OliI CACNNNNGTG 1 cut(s) 1823
PaeI GCATGC 3 cut(s) 9, 855, 2320
PaqCI CACCTGC 1 cut(s) 2251
PceI AGGCCT 1 cut(s) 2125
PciSI GCTCTTC 1 cut(s) 1308
PcsI WCGNNNNNNNCGW 1 cut(s) 1062
PctI GAATGC 2 cut(s) 27, 113
PdiI GCCGGC 1 cut(s) 733
PfeI GAWTC 4 cut(s) 555, 2011, 2026, 2323
PflFI GACNNNGTC 1 cut(s) 325
PkrI GCNGC 8 cut(s) 49, 70, 88, 91, 692, 823, 1294, 1654
PleI GAGTC 2 cut(s) 353, 1941
PpsI GAGTC 2 cut(s) 353, 1941
PshBI ATTAAT 1 cut(s) 1470
PsiI TTATAA 2 cut(s) 1169, 2291
Psp6I CCWGG 1 cut(s) 181
PspEI GGTNACC 1 cut(s) 319
PspGI CCWGG 1 cut(s) 181
PspN4I GGNNCC 8 cut(s) 646, 674, 929, 1520, 1620, 1669, 1794, 1844
PspOMI GGGCCC 1 cut(s) 672
PspPI GGNCC 5 cut(s) 342, 672, 673, 1283, 1619
PsuI RGATCY 2 cut(s) 487, 1667
PsyI GACNNNGTC 1 cut(s) 325
PvuII CAGCTG 2 cut(s) 89, 1813
RsaI GTAC 6 cut(s) 371, 646, 935, 1229, 1834, 1844
RsaNI GTAC 6 cut(s) 370, 645, 934, 1228, 1833, 1843
RseI CAYNNNNRTG 5 cut(s) 290, 1424, 1506, 1716, 1823
SalI GTCGAC 1 cut(s) 878
SapI GCTCTTC 1 cut(s) 1308
SaqAI TTAA 8 cut(s) 477, 747, 864, 977, 1145, 1320, 1470, 2102
SatI GCNGC 8 cut(s) 48, 69, 87, 90, 691, 822, 1293, 1653
Sau3AI GATC 8 cut(s) 207, 388, 487, 1647, 1667, 1801, 2020, 2032
Sau96I GGNCC 5 cut(s) 342, 672, 673, 1283, 1619
ScaI AGTACT 1 cut(s) 1229
SchI GAGTC 2 cut(s) 353, 1942
ScrFI CCNGG 1 cut(s) 183
SduI GDGCHC 6 cut(s) 387, 587, 676, 994, 1596, 1713
SfaNI GCATC 3 cut(s) 181, 2191, 2191
SfcI CTRYAG 3 cut(s) 985, 1048, 1897
SfuI TTCGAA 1 cut(s) 1784
SinI GGWCC 2 cut(s) 1283, 1619
SmiMI CAYNNNNRTG 5 cut(s) 290, 1424, 1506, 1716, 1823
SmlI CTYRAG 3 cut(s) 93, 1287, 1319
SmoI CTYRAG 3 cut(s) 93, 1287, 1319
SphI GCATGC 3 cut(s) 9, 855, 2320
SseBI AGGCCT 1 cut(s) 2125
SsiI CCGC 7 cut(s) 48, 101, 519, 843, 883, 1188, 1622
SspI AATATT 1 cut(s) 619
StuI AGGCCT 1 cut(s) 2125
StyD4I CCNGG 1 cut(s) 181
StyI CCWWGG 2 cut(s) 652, 1522
TaaI ACNGT 2 cut(s) 338, 625
TaiI ACGT 3 cut(s) 329, 2000, 2259
TaqI TCGA 4 cut(s) 387, 455, 879, 1784
TaqII GACCGA 1 cut(s) 2234
TatI WGTACW 2 cut(s) 369, 1227
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 4 cut(s) 555, 2011, 2026, 2323
Tru1I TTAA 8 cut(s) 477, 747, 864, 977, 1145, 1320, 1470, 2102
Tru9I TTAA 8 cut(s) 477, 747, 864, 977, 1145, 1320, 1470, 2102
TscAI CASTG 6 cut(s) 57, 385, 674, 911, 1137, 1881
TseFI GTSAC 5 cut(s) 319, 481, 806, 1037, 2238
TseI GCWGC 7 cut(s) 68, 86, 89, 690, 821, 1292, 1652
Tsp45I GTSAC 5 cut(s) 319, 481, 806, 1037, 2238
TspGWI ACGGA 4 cut(s) 135, 424, 677, 1296
TspRI CASTG 6 cut(s) 57, 385, 674, 911, 1137, 1881
Tth111I GACNNNGTC 1 cut(s) 325
Vha464I CTTAAG 1 cut(s) 1319
VneI GTGCAC 1 cut(s) 1709
VpaK11BI GGWCC 2 cut(s) 1283, 1619
VspI ATTAAT 1 cut(s) 1470
XapI RAATTY 3 cut(s) 542, 1125, 1273
XceI RCATGY 3 cut(s) 9, 855, 2320
XcmI CCANNNNNNNNNTGG 1 cut(s) 289
XmaJI CCTAGG 1 cut(s) 1522
XmiI GTMKAC 1 cut(s) 879
ZrmI AGTACT 1 cut(s) 1229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.