Rroxscaffold_1G00058900

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000066
Physical Location & Seq
Forward (+)
80852802 .. 80855864
3063 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00058900.1

Sequence Viewer

Length: 2226 bp
ATGGTCTTATACCGGAGAATGGTCATTTCGCAGCTGATCTCATTGGTAGTGGTGGCCATAGCTGCAGTAGCAATAGCCGACCAAGCCCTCCCGCCTCCTCAAGCCCTCCCTGAATGCCTTGACCACTGTGGCAATCTCACAGTCCCCTATCCATTCGGCATAGGCGACGGTTGTTACCTTACAAAAAAATTCAACCTCACCTGTAACGAATCCAGCCAACCTCCAACACTACTCTGGGCGGATGGTGGTATCACTACTACTAACATATCCGTGGTCGACGGTGAGTTGCAAATTTTGGCTTACATAGCCCGTGATTGTTACGACCAAACGGGTCATCAAGGCATGTACAATTTCCCATGGCTCTGGGTCTCTCCTCCGTTCACCGTCTCTGGTACTAGAAACAAGTTCATTGCCGTTGGCTATGACACCTATGCCGTTTTTAGAGGGTACCGCCATTCCCCTAATGATAAGGCTAGGCACGTAACTGGATGCATGTCTGTAAGTGACATTCTTGACAATACCACTACTGCTGCTAACGAGCCATGCAAAGGCATTGGCTACGCTTTCATTGTGGAAGAAGGCAAGTTCACATTTAACCCGAATACAAGTTTTCAAGAACTGCAAAACACTGAACTGCTTCCTCTGATTCTTAATTGGGAAATCGCGGACGGGTCATGTGCTGAAGCTCAAAAGAGGCAGGATTATGCTTGCAAGGCTCATAGCAAGTGTGTCAACCGGACCATCGACATCAAGACTAAACCGTCTGGTTATTATTGCCAGTGCTTGCCAGGCTACCAAGGGAACCCATATCTCCCAGATGGTTGTCAAGATATTGATGAGTGCATCGCTTCAACGAACCCCTGCAACAATGGAACATGCACAAATTCAGATGGAGATTACACATGTACGTGTAACAAAGGTTTCAGAAACGACAACCCAAAGAGTTGCATACAGAACACTCCTATATCAAAGAACAAGTCCCTGAAAGTTTCCTTGGGTGTCTCTCTGAGCTTCTTAGGAGTATTGCTTGTAACATTTTGGATATACTGCGGACTGAAGAGAAGAAAGTTCAAGATACTCAAAGAGAAGTTCTTCAAACACAATGGTGGCTACTTGTTACAACAAAAATTGGAGAATTTCGATGGACCTCAAGCAGCCAAAATATTTACTAGAGAAGAACTTAAGAAGGCCACAAACAACTTTCATGACAGTATAAAAATTGGTGAAGGAGGCTATGGATTTGTTTACAAAGGAACGCTATCAAATGGAAATGAGGTTGCCATAAAGGTGTCAAAAAGTAGTGCCCCAATGACCCAAAGTAATCAGTTCATTAATGAGGTGATTGTTCTTTCTCAGATCAACCACAGAAATGTGGTGAAGCTTCTAGGTTGTTGTTTGGAGACCCAAACGCCTTTATTAGTGTATGAGTTCGTCAGCAACGGCACACTTTATGAGCATATTCATAAAAAGAATGGCAAGGGACCGCTTTCCTTTGGGCTACGAATGAAGATAGCAACAGAAACAGCAGGATCACTAGCATACTTGCACTACTCTACTTCGATGCAAATTGTGCACCGAGATGTGAAGGCAGCAAACATTCTACTGGATGAGAATTTCACAGCGAAAGTGTCAGACTTTGGAGCTTCAAAATTGGTTCCCGAAGATCAAAATCAACTGTCCACTTTAGTGCAAGGGACGATAGGGTACTTGGACCCTGAATATCTTCAGTCAAACACACTGACAGAGAAAAGTGACGTATATAGCTTCGGCGTTGTCCTTGTGGAGCTAATAACAAGTCAAGTGGCAATTTCTTATCAGAAGCCTGAGGCGGAGAGAAGCTTAGCAAAATTTTTTGTTACTTCTGTGGAAGAGAATCGCCTAGATCAGATTCTCGATCATGAAATAATCAAAGAGGGGAGCTTCGAAACAGCTGAACAAGTAGCCCATCTGGCCAAAAGTTGTTTAAGTTTGAATGGGAGCGATAGGCCTACCATGAAAGAAGTAGCAATGAAGCTAGAGGGAGTATTGCAAGTTATGGCAAAGCATCCATGGGGAAAGGCTGATGCCTCACCAAAAGAGACTGACTACTTGCAGCTTGCGTCACATTCAAAAGCCTACGTTGTTGATGTTAGAGGTGATGAAGGTGAAATTATAACCAGTATTGACTACGACCGGAGCATCCAGATTCAACCCCTGATGATGAAGCCTTATGATGGGGGCCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK cEGF

Protein Analysis

741

Amino Acids

82.08

Weight (kDa)

6.27

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 39 - 97 2.1e-09 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 277 - 316 2.4e-07 Calcium-binding EGF domain
Pkinase PF00069 402 - 669 1.5e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 406 - 672 2.4e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2153
AasI GACNNNNNNGTC 1 cut(s) 760
Acc65I GGTACC 1 cut(s) 447
AccB1I GGYRCC 1 cut(s) 447
AccI GTMKAC 1 cut(s) 276
AccII CGCG 1 cut(s) 665
AciI CCGC 7 cut(s) 92, 239, 451, 665, 1050, 1484, 1829
AclWI GGATC 1 cut(s) 1537
AcoI YGGCCR 2 cut(s) 54, 1950
AcsI RAATTY 6 cut(s) 188, 291, 883, 1135, 1612, 1847
AcuI CTGAAG 3 cut(s) 702, 1076, 1709
AfaI GTAC 5 cut(s) 347, 394, 449, 907, 1706
AfiI CCNNNNNNNGG 3 cut(s) 19, 548, 2213
AflII CTTAAG 1 cut(s) 1181
AflIII ACRYGT 2 cut(s) 902, 908
AgsI TTSAA 9 cut(s) 193, 614, 852, 1072, 1096, 1647, 1972, 2109, 2189
AjnI CCWGG 1 cut(s) 787
AjuI GAANNNNNNNTTGG 2 cut(s) 977, 1009
AleI CACNNNNGTG 2 cut(s) 1104, 1685
Alw21I GWGCWC 1 cut(s) 1575
Alw26I GTCTC 5 cut(s) 373, 391, 1006, 1394, 2072
Alw44I GTGCAC 1 cut(s) 1571
AlwI GGATC 1 cut(s) 1537
AoxI GGCC 5 cut(s) 54, 1188, 1950, 1985, 2218
ApaLI GTGCAC 1 cut(s) 1571
ApeKI GCWGC 6 cut(s) 31, 62, 530, 1154, 1589, 2092
ApoI RAATTY 6 cut(s) 188, 291, 883, 1135, 1612, 1847
ArsI GACNNNNNNTTYG 2 cut(s) 962, 994
AseI ATTAAT 1 cut(s) 1332
Asp700I GAANNNNTTC 3 cut(s) 636, 1091, 1722
Asp718I GGTACC 1 cut(s) 447
AspS9I GGNCC 5 cut(s) 738, 1145, 1481, 1711, 2218
AsuHPI GGTGA 9 cut(s) 190, 293, 373, 1235, 1351, 1387, 2061, 2147, 2156
AsuII TTCGAA 1 cut(s) 1923
AvaII GGWCC 4 cut(s) 738, 1145, 1481, 1711
AxyI CCTNAGG 1 cut(s) 1824
BaeGI GKGCMC 2 cut(s) 1306, 1575
BalI TGGCCA 2 cut(s) 56, 1952
BanI GGYRCC 1 cut(s) 447
Bbv12I GWGCWC 1 cut(s) 1575
BbvI GCAGC 6 cut(s) 43, 49, 517, 1166, 1601, 2104
BccI CCATC 7 cut(s) 236, 749, 812, 884, 1136, 1953, 2207
BceAI ACGGC 3 cut(s) 398, 419, 1456
BciT130I CCWGG 1 cut(s) 789
BcoDI GTCTC 5 cut(s) 373, 391, 1006, 1394, 2072
BfaI CTAG 7 cut(s) 396, 474, 1170, 1385, 1535, 1880, 2015
BfmI CTRYAG 1 cut(s) 63
BfrI CTTAAG 1 cut(s) 1181
BglI GCCNNNNNGGC 1 cut(s) 1949
BisI GCNGC 6 cut(s) 32, 63, 531, 1155, 1590, 2093
BlpI GCTNAGC 1 cut(s) 1840
BlsI GCNGC 6 cut(s) 33, 64, 532, 1156, 1591, 2094
Bme1390I CCNGG 1 cut(s) 789
Bme18I GGWCC 4 cut(s) 738, 1145, 1481, 1711
BmgT120I GGNCC 5 cut(s) 738, 1145, 1481, 1711, 2218
BmiI GGNNCC 6 cut(s) 449, 803, 1482, 1656, 1713, 2219
BmrFI CCNGG 1 cut(s) 789
BmsI GCATC 6 cut(s) 479, 852, 1551, 2053, 2053, 2187
Bpu1102I GCTNAGC 1 cut(s) 1840
Bpu14I TTCGAA 1 cut(s) 1923
BpuEI CTTGAG 2 cut(s) 84, 1134
BsaAI YACGTR 2 cut(s) 481, 909
BsaBI GATNNNNATC 1 cut(s) 1668
BsaI GGTCTC 2 cut(s) 373, 1394
BsaJI CCNNGG 5 cut(s) 270, 356, 796, 993, 2048
BsaWI WCCGGW 3 cut(s) 12, 735, 2172
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 548, 2213
Bse1I ACTGG 4 cut(s) 490, 778, 1608, 2157
Bse21I CCTNAGG 1 cut(s) 1824
Bse3DI GCAATG 2 cut(s) 408, 2013
Bse8I GATNNNNATC 1 cut(s) 1668
BseBI CCWGG 1 cut(s) 789
BseDI CCNNGG 5 cut(s) 270, 356, 796, 993, 2048
BseGI GGATG 5 cut(s) 247, 494, 1612, 2044, 2178
BseJI GATNNNNATC 1 cut(s) 1668
BseLI CCNNNNNNNGG 3 cut(s) 19, 548, 2213
BseMI GCAATG 2 cut(s) 408, 2013
BseMII CTCAG 3 cut(s) 998, 1367, 1815
BseNI ACTGG 4 cut(s) 490, 778, 1608, 2157
BseRI GAGGAG 2 cut(s) 87, 363
BseSI GKGCMC 2 cut(s) 1306, 1575
BseXI GCAGC 6 cut(s) 43, 49, 517, 1166, 1601, 2104
Bsh1236I CGCG 1 cut(s) 665
Bsh1285I CGRYCG 1 cut(s) 2173
BshFI GGCC 5 cut(s) 56, 1190, 1952, 1987, 2220
BshNI GGYRCC 1 cut(s) 447
BsiEI CGRYCG 1 cut(s) 2173
BsiHKAI GWGCWC 1 cut(s) 1575
BsiSI CCGG 3 cut(s) 13, 736, 2173
BslFI GGGAC 4 cut(s) 128, 964, 1494, 1708
BslI CCNNNNNNNGG 3 cut(s) 19, 548, 2213
BsmAI GTCTC 5 cut(s) 373, 391, 1006, 1394, 2072
BsmBI CGTCTC 1 cut(s) 391
BsmFI GGGAC 4 cut(s) 128, 964, 1494, 1708
BsmI GAATGC 1 cut(s) 119
BsnI GGCC 5 cut(s) 56, 1190, 1952, 1987, 2220
Bso31I GGTCTC 2 cut(s) 373, 1394
Bsp119I TTCGAA 1 cut(s) 1923
Bsp1286I GDGCHC 2 cut(s) 1306, 1575
Bsp1407I TGTACA 1 cut(s) 345
Bsp143I GATC 6 cut(s) 36, 1356, 1529, 1663, 1882, 1894
Bsp1720I GCTNAGC 1 cut(s) 1840
Bsp19I CCATGG 2 cut(s) 356, 2048
BspACI CCGC 7 cut(s) 92, 239, 451, 665, 1050, 1484, 1829
BspANI GGCC 5 cut(s) 56, 1190, 1952, 1987, 2220
BspCNI CTCAG 3 cut(s) 999, 1366, 1816
BspFNI CGCG 1 cut(s) 665
BspHI TCATGA 2 cut(s) 1204, 1897
BspLI GGNNCC 6 cut(s) 449, 803, 1482, 1656, 1713, 2219
BspMAI CTGCAG 1 cut(s) 67
BspPI GGATC 1 cut(s) 1537
BspT104I TTCGAA 1 cut(s) 1923
BspT107I GGYRCC 1 cut(s) 447
BspTI CTTAAG 1 cut(s) 1181
BspTNI GGTCTC 2 cut(s) 373, 1394
BsrDI GCAATG 2 cut(s) 408, 2013
BsrGI TGTACA 1 cut(s) 345
BsrI ACTGG 4 cut(s) 490, 778, 1608, 2157
BssECI CCNNGG 5 cut(s) 270, 356, 796, 993, 2048
BssMI GATC 6 cut(s) 36, 1356, 1529, 1663, 1882, 1894
BssT1I CCWWGG 4 cut(s) 356, 796, 993, 2048
Bst2UI CCWGG 1 cut(s) 789
Bst4CI ACNGT 8 cut(s) 128, 142, 170, 281, 385, 762, 1211, 1677
Bst6I CTCTTC 2 cut(s) 1052, 1863
BstAFI CTTAAG 1 cut(s) 1181
BstAPI GCANNNNNTGC 1 cut(s) 1570
BstAUI TGTACA 1 cut(s) 345
BstBAI YACGTR 2 cut(s) 481, 909
BstBI TTCGAA 1 cut(s) 1923
BstC8I GCNNGC 3 cut(s) 709, 785, 2097
BstDEI CTNAG 5 cut(s) 1007, 1015, 1353, 1824, 1840
BstDSI CCRYGG 3 cut(s) 270, 356, 2048
BstF5I GGATG 5 cut(s) 247, 494, 1612, 2044, 2178
BstFNI CGCG 1 cut(s) 665
BstKTI GATC 6 cut(s) 39, 1359, 1532, 1666, 1885, 1897
BstMAI GTCTC 5 cut(s) 373, 391, 1006, 1394, 2072
BstMBI GATC 6 cut(s) 36, 1356, 1529, 1663, 1882, 1894
BstMCI CGRYCG 1 cut(s) 2173
BstMWI GCNNNNNNNGC 8 cut(s) 62, 68, 83, 305, 713, 789, 1570, 1949
BstNI CCWGG 1 cut(s) 789
BstNSI RCATGY 4 cut(s) 346, 496, 879, 906
BstSCI CCNGG 1 cut(s) 787
BstSFI CTRYAG 1 cut(s) 63
BstSLI GKGCMC 2 cut(s) 1306, 1575
BstUI CGCG 1 cut(s) 665
BstV1I GCAGC 6 cut(s) 43, 49, 517, 1166, 1601, 2104
BstXI CCANNNNNNTGG 1 cut(s) 363
Bsu36I CCTNAGG 1 cut(s) 1824
BsuRI GGCC 5 cut(s) 56, 1190, 1952, 1987, 2220
BtgI CCRYGG 3 cut(s) 270, 356, 2048
BtgZI GCGATG 1 cut(s) 829
BtsCI GGATG 5 cut(s) 247, 494, 1612, 2044, 2178
BtsIMutI CAGTG 4 cut(s) 124, 627, 785, 1736
Cac8I GCNNGC 3 cut(s) 709, 785, 2097
CciI TCATGA 2 cut(s) 1204, 1897
Cfr13I GGNCC 5 cut(s) 738, 1145, 1481, 1711, 2218
CseI GACGC 1 cut(s) 2088
Csp6I GTAC 5 cut(s) 346, 393, 448, 906, 1705
CspCI CAANNNNNGTGG 2 cut(s) 1782, 1817
CviQI GTAC 5 cut(s) 346, 393, 448, 906, 1705
DdeI CTNAG 5 cut(s) 1007, 1015, 1353, 1824, 1840
DpnI GATC 6 cut(s) 38, 1358, 1531, 1665, 1884, 1896
DpnII GATC 6 cut(s) 36, 1356, 1529, 1663, 1882, 1894
DrdI GACNNNNNNGTC 1 cut(s) 760
DseDI GACNNNNNNGTC 1 cut(s) 760
EaeI YGGCCR 2 cut(s) 54, 1950
Eam1104I CTCTTC 2 cut(s) 1052, 1863
EarI CTCTTC 2 cut(s) 1052, 1863
EciI GGCGGA 2 cut(s) 254, 1844
Eco130I CCWWGG 4 cut(s) 356, 796, 993, 2048
Eco147I AGGCCT 1 cut(s) 1987
Eco31I GGTCTC 2 cut(s) 373, 1394
Eco47I GGWCC 4 cut(s) 738, 1145, 1481, 1711
Eco57I CTGAAG 3 cut(s) 702, 1076, 1709
Eco81I CCTNAGG 1 cut(s) 1824
EcoRII CCWGG 1 cut(s) 787
EcoT14I CCWWGG 4 cut(s) 356, 796, 993, 2048
EcoT22I ATGCAT 1 cut(s) 494
ErhI CCWWGG 4 cut(s) 356, 796, 993, 2048
Esp3I CGTCTC 1 cut(s) 391
FaqI GGGAC 4 cut(s) 128, 964, 1494, 1708
FauI CCCGC 1 cut(s) 99
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 6 cut(s) 32, 63, 531, 1155, 1590, 2093
FokI GGATG 5 cut(s) 254, 501, 1619, 2031, 2165
Fsp4HI GCNGC 6 cut(s) 32, 63, 531, 1155, 1590, 2093
FspBI CTAG 7 cut(s) 396, 474, 1170, 1385, 1535, 1880, 2015
GluI GCNGC 6 cut(s) 32, 63, 531, 1155, 1590, 2093
HaeIII GGCC 5 cut(s) 56, 1190, 1952, 1987, 2220
HapII CCGG 3 cut(s) 13, 736, 2173
HgaI GACGC 1 cut(s) 2088
HincII GTYRAC 2 cut(s) 277, 733
HindII GTYRAC 2 cut(s) 277, 733
HindIII AAGCTT 2 cut(s) 1379, 1837
HinfI GANTC 5 cut(s) 209, 646, 1873, 1888, 2185
HpaII CCGG 3 cut(s) 13, 736, 2173
HphI GGTGA 9 cut(s) 190, 293, 373, 1235, 1351, 1387, 2061, 2147, 2156
Hpy166II GTNNAC 7 cut(s) 277, 381, 588, 733, 1246, 1573, 1680
Hpy188I TCNGA 8 cut(s) 645, 889, 926, 1008, 1356, 1633, 1818, 1887
Hpy8I GTNNAC 7 cut(s) 277, 381, 588, 733, 1246, 1573, 1680
Hpy99I CGWCG 2 cut(s) 170, 281
HpyAV CCTTC 5 cut(s) 572, 1180, 1220, 1579, 2135
HpyCH4III ACNGT 8 cut(s) 128, 142, 170, 281, 385, 762, 1211, 1677
HpyCH4IV ACGT 4 cut(s) 480, 908, 1755, 2118
HpyF10VI GCNNNNNNNGC 8 cut(s) 62, 68, 83, 305, 713, 789, 1570, 1949
HpyF3I CTNAG 5 cut(s) 1007, 1015, 1353, 1824, 1840
HpySE526I ACGT 4 cut(s) 480, 908, 1755, 2118
KpnI GGTACC 1 cut(s) 451
Kzo9I GATC 6 cut(s) 36, 1356, 1529, 1663, 1882, 1894
LmnI GCTCC 5 cut(s) 1640, 1783, 1917, 1977, 2175
Lsp1109I GCAGC 6 cut(s) 43, 49, 517, 1166, 1601, 2104
LweI GCATC 6 cut(s) 479, 852, 1551, 2053, 2053, 2187
MaeI CTAG 7 cut(s) 396, 474, 1170, 1385, 1535, 1880, 2015
MaeII ACGT 4 cut(s) 480, 908, 1755, 2118
MalI GATC 6 cut(s) 38, 1358, 1531, 1665, 1884, 1896
MboI GATC 6 cut(s) 36, 1356, 1529, 1663, 1882, 1894
MboII GAAGA 9 cut(s) 587, 1069, 1074, 1084, 1187, 1519, 1673, 1715, 1880
MhlI GDGCHC 2 cut(s) 1306, 1575
MlsI TGGCCA 2 cut(s) 56, 1952
MluNI TGGCCA 2 cut(s) 56, 1952
MmeI TCCRAC 1 cut(s) 248
Mox20I TGGCCA 2 cut(s) 56, 1952
Mph1103I ATGCAT 1 cut(s) 494
MroXI GAANNNNTTC 3 cut(s) 636, 1091, 1722
MscI TGGCCA 2 cut(s) 56, 1952
MseI TTAA 5 cut(s) 594, 651, 1182, 1332, 1964
MslI CAYNNNNRTG 7 cut(s) 269, 907, 1104, 1286, 1368, 1578, 1685
Msp20I TGGCCA 2 cut(s) 56, 1952
MspA1I CMGCKG 2 cut(s) 34, 1931
MspCI CTTAAG 1 cut(s) 1181
MspI CCGG 3 cut(s) 13, 736, 2173
MspR9I CCNGG 1 cut(s) 789
Mva1269I GAATGC 1 cut(s) 119
MvaI CCWGG 1 cut(s) 789
MvnI CGCG 1 cut(s) 665
MwoI GCNNNNNNNGC 8 cut(s) 62, 68, 83, 305, 713, 789, 1570, 1949
NcoI CCATGG 2 cut(s) 356, 2048
NdeII GATC 6 cut(s) 36, 1356, 1529, 1663, 1882, 1894
NlaIV GGNNCC 6 cut(s) 449, 803, 1482, 1656, 1713, 2219
NmuCI GTSAC 3 cut(s) 503, 1751, 2100
NsiI ATGCAT 1 cut(s) 494
NspI RCATGY 4 cut(s) 346, 496, 879, 906
NspV TTCGAA 1 cut(s) 1923
OliI CACNNNNGTG 2 cut(s) 1104, 1685
PagI TCATGA 2 cut(s) 1204, 1897
PceI AGGCCT 1 cut(s) 1987
PciI ACATGT 1 cut(s) 902
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PctI GAATGC 1 cut(s) 119
PdmI GAANNNNTTC 3 cut(s) 636, 1091, 1722
PfeI GAWTC 5 cut(s) 209, 646, 1873, 1888, 2185
PkrI GCNGC 6 cut(s) 33, 64, 532, 1156, 1591, 2094
Ppu21I YACGTR 2 cut(s) 481, 909
PscI ACATGT 1 cut(s) 902
PshBI ATTAAT 1 cut(s) 1332
PsiI TTATAA 1 cut(s) 2153
Psp6I CCWGG 1 cut(s) 787
PspGI CCWGG 1 cut(s) 787
PspN4I GGNNCC 6 cut(s) 449, 803, 1482, 1656, 1713, 2219
PspPI GGNCC 5 cut(s) 738, 1145, 1481, 1711, 2218
PstI CTGCAG 1 cut(s) 67
PvuII CAGCTG 2 cut(s) 34, 1931
RsaI GTAC 5 cut(s) 347, 394, 449, 907, 1706
RsaNI GTAC 5 cut(s) 346, 393, 448, 906, 1705
RseI CAYNNNNRTG 7 cut(s) 269, 907, 1104, 1286, 1368, 1578, 1685
SalI GTCGAC 1 cut(s) 275
SaqAI TTAA 5 cut(s) 594, 651, 1182, 1332, 1964
SatI GCNGC 6 cut(s) 32, 63, 531, 1155, 1590, 2093
Sau3AI GATC 6 cut(s) 36, 1356, 1529, 1663, 1882, 1894
Sau96I GGNCC 5 cut(s) 738, 1145, 1481, 1711, 2218
ScrFI CCNGG 1 cut(s) 789
SduI GDGCHC 2 cut(s) 1306, 1575
SfaNI GCATC 6 cut(s) 479, 852, 1551, 2053, 2053, 2187
SfcI CTRYAG 1 cut(s) 63
SfuI TTCGAA 1 cut(s) 1923
SinI GGWCC 4 cut(s) 738, 1145, 1481, 1711
SmiMI CAYNNNNRTG 7 cut(s) 269, 907, 1104, 1286, 1368, 1578, 1685
SmlI CTYRAG 3 cut(s) 99, 1149, 1181
SmoI CTYRAG 3 cut(s) 99, 1149, 1181
SseBI AGGCCT 1 cut(s) 1987
SsiI CCGC 7 cut(s) 92, 239, 451, 665, 1050, 1484, 1829
SspI AATATT 1 cut(s) 1164
SspMI CTAG 7 cut(s) 396, 474, 1170, 1385, 1535, 1880, 2015
StuI AGGCCT 1 cut(s) 1987
StyD4I CCNGG 1 cut(s) 787
StyI CCWWGG 4 cut(s) 356, 796, 993, 2048
TaaI ACNGT 8 cut(s) 128, 142, 170, 281, 385, 762, 1211, 1677
TaiI ACGT 4 cut(s) 483, 911, 1758, 2121
TaqI TCGA 6 cut(s) 276, 744, 1140, 1559, 1893, 1923
TatI WGTACW 1 cut(s) 345
TfiI GAWTC 5 cut(s) 209, 646, 1873, 1888, 2185
Tru1I TTAA 5 cut(s) 594, 651, 1182, 1332, 1964
Tru9I TTAA 5 cut(s) 594, 651, 1182, 1332, 1964
TscAI CASTG 4 cut(s) 131, 634, 785, 1743
TseFI GTSAC 3 cut(s) 503, 1751, 2100
TseI GCWGC 6 cut(s) 31, 62, 530, 1154, 1589, 2092
Tsp45I GTSAC 3 cut(s) 503, 1751, 2100
TspGWI ACGGA 2 cut(s) 259, 366
TspRI CASTG 4 cut(s) 131, 634, 785, 1743
Vha464I CTTAAG 1 cut(s) 1181
VneI GTGCAC 1 cut(s) 1571
VpaK11BI GGWCC 4 cut(s) 738, 1145, 1481, 1711
VspI ATTAAT 1 cut(s) 1332
XapI RAATTY 6 cut(s) 188, 291, 883, 1135, 1612, 1847
XceI RCATGY 4 cut(s) 346, 496, 879, 906
XcmI CCANNNNNNNNNTGG 1 cut(s) 231
XmiI GTMKAC 1 cut(s) 276
XmnI GAANNNNTTC 3 cut(s) 636, 1091, 1722
XspI CTAG 7 cut(s) 396, 474, 1170, 1385, 1535, 1880, 2015
Zsp2I ATGCAT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.