Rroxscaffold_1G00025870

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000066
Physical Location & Seq
Reverse (-)
32476925 .. 32479938
3014 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00025870.1

Sequence Viewer

Length: 2295 bp
ATGGCTGTGCACGGGAGGATGCTAGTTGTGCAACTGATCATAGCGGTGGCCATAGCAGCGGCAGCAGCTGATCGAACCCCACCTCCTCAAGCACTACCTGGCTGCCCCGACCACTGTGGTAATCTCACAATCCCATATCCTTTCGGCATCGGGGAGGGTTGTTTCCAGCGAGAATGGTTCAACCTCACTTGTAACGAATCCGCCGAACCCCCAACAACACTCTGGATCGATGGTAGTTTCACGGTTACTAACATATCGCTTGCTGAAGGTGAGTTGGAGGTTATGAATTACATAGCCCATGATTGTTATGATCAGCACGGTAATCAAACAGAAGACATGTACAATTCCCCTTATCTCTGGTTGTATCCTCCTTTCACCATCTCCGGGACCAGAAACAAGTTTATTGCCGTTGGCTGCGACACTTATGCAGTTTTTGAAGGGAACCGCCGTGACGAACATAGGTACATCACCGGATGCATGTCTGTATGTGACAATCTTGACAGTGCAGTTAACGGTTCGTGCTCCGGCATTGGGTGTTGCCAAACTTCCATCCCTAGCGGACTCAAAAATCGCACCGTCGGATTGGATACCTATTTTTATCACAAGGACGTGTGGGACTTCAATCCATGCAGCTACGCCTTCATTGTGGCAGAAGGCAGGTTCACATTCAACCCTAATACAAGTTTTGAAGAACTCAACAACACTGAACAACTTCCCATGATTATTAACTGGGATTTTAATGAAAGGTCATGTGCTCTAGCTCAAAACAGCCCTGGTTATGCTTGCAAAGCAAATAGCAGCTGTGTCAATCGGACCTTCGATAACCAGACCGAGCCAACTGGTTATTATTGCCGGTGCTTGCCAGGCTTCGAAGGGAACCCATATCTTCCAGATGGTTGCCAAGATATTGACGAGTGCATGGCTCCATCAAGTCCATGCAACAATGGAATATGCCAAAATTTACCTGGAGATTACAGCTGTTTATGTAACAAAGGTTTCAAAAACCAGGACACAAAGAATTGCATAGAAAACACTCCCGTGCCAAAGAACATGCCCCTGAAAATTTCGTTGGGTCTCAGTATAGGCTTCTTCATTTTAGTGGTTTTAATTTGGTGGACTTATTGGGGAATGAACAAACGAAGACTAGTAAGACTCAAAGAAAAGTACTTCAAAGAAAATGGCGGCTCAACATTACAGCAACATCTCGCTAGTCATGGAGGATCTGTTGAGACAGCAAAAATCTTTACTGCAGAGGAACTTCAGAAAGCCACAAACAATTACCATGTAGATGAAATCCTTGGTGAAGGAGGCTATGCAACAGTTTACAAAGGAATACTACATGATAAGAGTGTTGTTGCCATAAAGAAGCCGAAGATTGGCGCTCCAACTCAAAGTGATAAGTTCATTAATGAGGTTATCGTTCTTTCTCAGATAAACCACAGAAATGTAGTAAAACTATTAGGTTGTTGTTTCGAGACCGAAGTGCCTTTACTGGTATATGAGTACATTACTCATGGCACTCTTTTCGAGCATGTATTCAATAAGGGAAAAGGATCCTCGCTTTCATGGGAATTACGACTGAAGATAGCATCAGAAACTGCTGACGCACTAGCATACTTACACTCATCTACTTCCACTCCAATCATACATAGAGACGTGAAAACAACGAATATATTATTGGATGACAATTACACGGCCAAAGTGTCTGATTTTGGAGCTTCGCGTTTCATTCCTATAGATCAAACTCAACTTGCAACACTAGTTCAAGGGACACTTGGATACTTGGATCCTGAATACTTTCATTCAAACCAACTAACTGAAAAGAGTGATGTCTATAGTTTTGGAGTTGTCCTAGCGGAGCTACTAACAAGTAGAGTTGCACTTTCTTTTGCCAGGCCTGACAAGGAGAGATGCCTAGCAAGCTTCTTTGTTTCTTCAATAGAAAACGAATGCTTGATTCAAATTCTTGATGCTGGTATAGTGAATGAGGGGAACATAGAGACAGTAGAGCAAGTGGCGAATCTGGCAAAAAGATGTTTGATGATAAAAAGTGAGGAAAGGCCGACGATGAGAGAAGTTGCCACGGAGTTAGAAGGGATGAGAATTATGGCAACGCATCCATGGAAAAATGGCAATTCCGGCTTTGAGGAGACTGAGTACTTGCTCGGGTCACCTAATTCAGCGGCTTATGTGATGAATATTGAAGATGGAGGTGCCGGTGGTTCGAGCGTTACAAATACTGGGTATGACAGCATGCAAATTCAAATGTTAATGCCCTACGATGACGGGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

764

Amino Acids

84.77

Weight (kDa)

5.04

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 95 8.7e-14 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 302 - 341 7.8e-08 Calcium-binding EGF domain
Pkinase PF00069 427 - 694 2.5e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 429 - 697 9.5e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 648
AccB1I GGYRCC 1 cut(s) 2213
AccII CGCG 1 cut(s) 1723
AciI CCGC 8 cut(s) 44, 59, 201, 445, 558, 1182, 1856, 2183
AclWI GGATC 6 cut(s) 233, 1228, 1548, 1561, 1781, 1794
AcoI YGGCCR 2 cut(s) 48, 1695
AcsI RAATTY 4 cut(s) 958, 1062, 1962, 2259
AcuI CTGAAG 3 cut(s) 285, 1244, 1601
AfaI GTAC 5 cut(s) 341, 464, 1166, 1505, 2159
AfiI CCNNNNNNNGG 3 cut(s) 384, 531, 1376
AflIII ACRYGT 2 cut(s) 336, 609
AhlI ACTAGT 2 cut(s) 1144, 1759
AjiI CACGTC 2 cut(s) 610, 1657
AjnI CCWGG 6 cut(s) 97, 772, 862, 964, 1005, 1892
AjuI GAANNNNNNNTTGG 4 cut(s) 1052, 1084, 1661, 1693
AleI CACNNNNGTG 1 cut(s) 1037
AloI GAACNNNNNNTCC 2 cut(s) 67, 99
AluBI AGCT 8 cut(s) 68, 633, 761, 801, 978, 1718, 1861, 1923
AluI AGCT 8 cut(s) 68, 633, 761, 801, 978, 1718, 1861, 1923
Alw21I GWGCWC 3 cut(s) 12, 524, 757
Alw26I GTCTC 6 cut(s) 1079, 1223, 1469, 1647, 1994, 2144
Alw44I GTGCAC 1 cut(s) 8
AlwI GGATC 6 cut(s) 233, 1228, 1548, 1561, 1781, 1794
AlwNI CAGNNNCTG 2 cut(s) 68, 1598
Ama87I CYCGRG 1 cut(s) 2165
AoxI GGCC 4 cut(s) 48, 1695, 1895, 2060
ApaLI GTGCAC 1 cut(s) 8
ApeKI GCWGC 7 cut(s) 56, 62, 65, 102, 414, 630, 798
ApoI RAATTY 4 cut(s) 958, 1062, 1962, 2259
AseI ATTAAT 1 cut(s) 1407
Asp700I GAANNNNTTC 2 cut(s) 711, 1797
AspLEI GCGC 1 cut(s) 1382
AspS9I GGNCC 2 cut(s) 387, 813
AsuC2I CCSGG 1 cut(s) 385
AsuHPI GGTGA 5 cut(s) 281, 367, 460, 1313, 2163
AsuII TTCGAA 1 cut(s) 870
AvaI CYCGRG 1 cut(s) 2165
AvaII GGWCC 2 cut(s) 387, 813
BaeGI GKGCMC 1 cut(s) 12
BalI TGGCCA 1 cut(s) 50
BamHI GGATCC 2 cut(s) 1553, 1786
BanI GGYRCC 1 cut(s) 2213
BarI GAAGNNNNNNTAC 2 cut(s) 1473, 1505
BbsI GAAGAC 2 cut(s) 339, 1147
Bbv12I GWGCWC 3 cut(s) 12, 524, 757
BbvI GCAGC 7 cut(s) 68, 74, 77, 89, 401, 642, 810
BccI CCATC 6 cut(s) 224, 386, 557, 887, 934, 2201
BceAI ACGGC 3 cut(s) 392, 432, 1710
BcgI CGANNNNNNTGC 4 cut(s) 407, 441, 1507, 1541
BciT130I CCWGG 6 cut(s) 99, 774, 864, 966, 1007, 1894
BciVI GTATCC 3 cut(s) 375, 580, 1772
BclI TGATCA 2 cut(s) 36, 310
BcnI CCSGG 1 cut(s) 385
BcoDI GTCTC 6 cut(s) 1079, 1223, 1469, 1647, 1994, 2144
BcuI ACTAGT 2 cut(s) 1144, 1759
BfaI CTAG 9 cut(s) 23, 555, 758, 1145, 1209, 1610, 1760, 1853, 1916
BfmI CTRYAG 3 cut(s) 1248, 1734, 1834
BfoI RGCGCY 1 cut(s) 1383
BfuAI ACCTGC 1 cut(s) 648
BfuI GTATCC 3 cut(s) 375, 580, 1772
BmcAI AGTACT 2 cut(s) 1166, 2159
Bme1390I CCNGG 7 cut(s) 99, 385, 774, 864, 966, 1007, 1894
Bme18I GGWCC 2 cut(s) 387, 813
BmeT110I CYCGRG 1 cut(s) 2165
BmgBI CACGTC 2 cut(s) 610, 1657
BmgT120I GGNCC 2 cut(s) 387, 813
BmiI GGNNCC 7 cut(s) 388, 443, 878, 924, 1555, 1788, 2215
BmrFI CCNGG 7 cut(s) 99, 385, 774, 864, 966, 1007, 1894
BmrI ACTGGG 2 cut(s) 739, 2250
BmsI GCATC 7 cut(s) 9, 156, 464, 1598, 1901, 1960, 2125
BmuI ACTGGG 2 cut(s) 739, 2250
BpiI GAAGAC 2 cut(s) 339, 1147
BpmI CTGGAG 1 cut(s) 987
Bpu14I TTCGAA 1 cut(s) 870
BpuEI CTTGAG 1 cut(s) 72
BpuMI CCSGG 1 cut(s) 385
Bsa29I ATCGAT 1 cut(s) 228
BsaI GGTCTC 2 cut(s) 1079, 1469
BsaJI CCNNGG 4 cut(s) 772, 1297, 2082, 2120
BsaWI WCCGGW 1 cut(s) 470
BsaXI ACNNNNNCTCC 8 cut(s) 67, 97, 1209, 1239, 1621, 1651, 2141, 2171
Bsc4I CCNNNNNNNGG 3 cut(s) 384, 531, 1376
Bse118I RCCGGY 2 cut(s) 852, 2216
Bse1I ACTGG 4 cut(s) 734, 844, 1497, 2245
BseBI CCWGG 6 cut(s) 99, 774, 864, 966, 1007, 1894
BseCI ATCGAT 1 cut(s) 228
BseDI CCNNGG 4 cut(s) 772, 1297, 2082, 2120
BseGI GGATG 6 cut(s) 24, 479, 549, 1687, 2103, 2116
BseLI CCNNNNNNNGG 3 cut(s) 384, 531, 1376
BseMII CTCAG 3 cut(s) 1090, 1442, 2145
BseNI ACTGG 4 cut(s) 734, 844, 1497, 2245
BseRI GAGGAG 2 cut(s) 75, 2162
BseSI GKGCMC 1 cut(s) 12
BseXI GCAGC 7 cut(s) 68, 74, 77, 89, 401, 642, 810
BsgI GTGCAG 1 cut(s) 525
Bsh1236I CGCG 1 cut(s) 1723
BshFI GGCC 4 cut(s) 50, 1697, 1897, 2062
BshNI GGYRCC 1 cut(s) 2213
BshVI ATCGAT 1 cut(s) 228
BsiHKAI GWGCWC 3 cut(s) 12, 524, 757
BsiHKCI CYCGRG 1 cut(s) 2165
BsiSI CCGG 6 cut(s) 384, 471, 525, 853, 2139, 2217
BslFI GGGAC 3 cut(s) 400, 629, 1783
BslI CCNNNNNNNGG 3 cut(s) 384, 531, 1376
BsmAI GTCTC 6 cut(s) 1079, 1223, 1469, 1647, 1994, 2144
BsmBI CGTCTC 1 cut(s) 1647
BsmFI GGGAC 3 cut(s) 400, 629, 1783
BsmI GAATGC 1 cut(s) 1955
BsnI GGCC 4 cut(s) 50, 1697, 1897, 2062
Bso31I GGTCTC 2 cut(s) 1079, 1469
BsoBI CYCGRG 1 cut(s) 2165
Bsp119I TTCGAA 1 cut(s) 870
Bsp1286I GDGCHC 3 cut(s) 12, 524, 757
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 8 cut(s) 36, 70, 225, 310, 1220, 1553, 1738, 1786
Bsp19I CCATGG 1 cut(s) 2120
BspACI CCGC 8 cut(s) 44, 59, 201, 445, 558, 1182, 1856, 2183
BspANI GGCC 4 cut(s) 50, 1697, 1897, 2062
BspCNI CTCAG 3 cut(s) 1089, 1441, 2146
BspDI ATCGAT 1 cut(s) 228
BspFNI CGCG 1 cut(s) 1723
BspLI GGNNCC 7 cut(s) 388, 443, 878, 924, 1555, 1788, 2215
BspMAI CTGCAG 1 cut(s) 1252
BspMI ACCTGC 1 cut(s) 648
BspPI GGATC 6 cut(s) 233, 1228, 1548, 1561, 1781, 1794
BspT104I TTCGAA 1 cut(s) 870
BspT107I GGYRCC 1 cut(s) 2213
BspTNI GGTCTC 2 cut(s) 1079, 1469
BsrFI RCCGGY 2 cut(s) 852, 2216
BsrGI TGTACA 1 cut(s) 339
BsrI ACTGG 4 cut(s) 734, 844, 1497, 2245
BssAI RCCGGY 2 cut(s) 852, 2216
BssECI CCNNGG 4 cut(s) 772, 1297, 2082, 2120
BssMI GATC 8 cut(s) 36, 70, 225, 310, 1220, 1553, 1738, 1786
BssT1I CCWWGG 2 cut(s) 1297, 2120
Bst2UI CCWGG 6 cut(s) 99, 774, 864, 966, 1007, 1894
Bst4CI ACNGT 8 cut(s) 116, 244, 320, 503, 515, 577, 1321, 2005
BstAUI TGTACA 1 cut(s) 339
BstBI TTCGAA 1 cut(s) 870
BstC8I GCNNGC 5 cut(s) 261, 784, 860, 1921, 2255
BstDEI CTNAG 3 cut(s) 1076, 1428, 2154
BstDSI CCRYGG 2 cut(s) 2082, 2120
BstEII GGTNACC 1 cut(s) 2169
BstF5I GGATG 6 cut(s) 24, 479, 549, 1687, 2103, 2116
BstFNI CGCG 1 cut(s) 1723
BstH2I RGCGCY 1 cut(s) 1383
BstHHI GCGC 1 cut(s) 1382
BstKTI GATC 8 cut(s) 39, 73, 228, 313, 1223, 1556, 1741, 1789
BstMAI GTCTC 6 cut(s) 1079, 1223, 1469, 1647, 1994, 2144
BstMBI GATC 8 cut(s) 36, 70, 225, 310, 1220, 1553, 1738, 1786
BstMWI GCNNNNNNNGC 9 cut(s) 28, 56, 62, 65, 788, 864, 1920, 2024, 2139
BstNI CCWGG 6 cut(s) 99, 774, 864, 966, 1007, 1894
BstNSI RCATGY 5 cut(s) 340, 481, 1054, 1535, 2257
BstPI GGTNACC 1 cut(s) 2169
BstSCI CCNGG 7 cut(s) 97, 383, 772, 862, 964, 1005, 1892
BstSFI CTRYAG 3 cut(s) 1248, 1734, 1834
BstSLI GKGCMC 1 cut(s) 12
BstUI CGCG 1 cut(s) 1723
BstV1I GCAGC 7 cut(s) 68, 74, 77, 89, 401, 642, 810
BstV2I GAAGAC 2 cut(s) 339, 1147
BstX2I RGATCY 3 cut(s) 1220, 1553, 1786
BstYI RGATCY 3 cut(s) 1220, 1553, 1786
Bsu15I ATCGAT 1 cut(s) 228
BsuI GTATCC 3 cut(s) 375, 580, 1772
BsuRI GGCC 4 cut(s) 50, 1697, 1897, 2062
BsuTUI ATCGAT 1 cut(s) 228
BtgI CCRYGG 2 cut(s) 2082, 2120
BtrI CACGTC 2 cut(s) 610, 1657
BtsCI GGATG 6 cut(s) 24, 479, 549, 1687, 2103, 2116
BtsIMutI CAGTG 3 cut(s) 112, 508, 702
BveI ACCTGC 1 cut(s) 648
Cac8I GCNNGC 5 cut(s) 261, 784, 860, 1921, 2255
CaiI CAGNNNCTG 2 cut(s) 68, 1598
CfoI GCGC 1 cut(s) 1382
Cfr10I RCCGGY 2 cut(s) 852, 2216
Cfr13I GGNCC 2 cut(s) 387, 813
ClaI ATCGAT 1 cut(s) 228
CseI GACGC 1 cut(s) 1613
Csp6I GTAC 5 cut(s) 340, 463, 1165, 1504, 2158
CviQI GTAC 5 cut(s) 340, 463, 1165, 1504, 2158
DdeI CTNAG 3 cut(s) 1076, 1428, 2154
DpnI GATC 8 cut(s) 38, 72, 227, 312, 1222, 1555, 1740, 1788
DpnII GATC 8 cut(s) 36, 70, 225, 310, 1220, 1553, 1738, 1786
EaeI YGGCCR 2 cut(s) 48, 1695
EciI GGCGGA 1 cut(s) 190
Eco130I CCWWGG 2 cut(s) 1297, 2120
Eco147I AGGCCT 1 cut(s) 1897
Eco31I GGTCTC 2 cut(s) 1079, 1469
Eco47I GGWCC 2 cut(s) 387, 813
Eco57I CTGAAG 3 cut(s) 285, 1244, 1601
Eco88I CYCGRG 1 cut(s) 2165
Eco91I GGTNACC 1 cut(s) 2169
EcoO65I GGTNACC 1 cut(s) 2169
EcoRII CCWGG 6 cut(s) 97, 772, 862, 964, 1005, 1892
EcoT14I CCWWGG 2 cut(s) 1297, 2120
EcoT22I ATGCAT 1 cut(s) 479
ErhI CCWWGG 2 cut(s) 1297, 2120
Esp3I CGTCTC 1 cut(s) 1647
FalI AAGNNNNNCTT 2 cut(s) 1758, 1790
FaqI GGGAC 3 cut(s) 400, 629, 1783
FbaI TGATCA 2 cut(s) 36, 310
FokI GGATG 6 cut(s) 31, 486, 536, 1694, 2103, 2110
FspBI CTAG 9 cut(s) 23, 555, 758, 1145, 1209, 1610, 1760, 1853, 1916
GlaI GCGC 1 cut(s) 1381
GsuI CTGGAG 1 cut(s) 987
HaeII RGCGCY 1 cut(s) 1383
HaeIII GGCC 4 cut(s) 50, 1697, 1897, 2062
HapII CCGG 6 cut(s) 384, 471, 525, 853, 2139, 2217
HgaI GACGC 1 cut(s) 1613
HhaI GCGC 1 cut(s) 1382
Hin6I GCGC 1 cut(s) 1380
HinP1I GCGC 1 cut(s) 1380
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HindIII AAGCTT 1 cut(s) 1921
HinfI GANTC 5 cut(s) 197, 561, 1152, 1957, 2020
HpaI GTTAAC 1 cut(s) 511
HpaII CCGG 6 cut(s) 384, 471, 525, 853, 2139, 2217
HphI GGTGA 5 cut(s) 281, 367, 460, 1313, 2163
Hpy166II GTNNAC 5 cut(s) 10, 511, 663, 1116, 1324
Hpy188I TCNGA 6 cut(s) 581, 813, 1263, 1431, 1594, 1708
Hpy188III TCNNGA 6 cut(s) 223, 497, 890, 1474, 1790, 1967
Hpy8I GTNNAC 5 cut(s) 10, 511, 663, 1116, 1324
Hpy99I CGWCG 2 cut(s) 581, 2068
HpyAV CCTTC 8 cut(s) 260, 431, 647, 649, 826, 866, 1298, 2087
HpyCH4III ACNGT 8 cut(s) 116, 244, 320, 503, 515, 577, 1321, 2005
HpyCH4IV ACGT 2 cut(s) 609, 1656
HpyF10VI GCNNNNNNNGC 9 cut(s) 28, 56, 62, 65, 788, 864, 1920, 2024, 2139
HpyF3I CTNAG 3 cut(s) 1076, 1428, 2154
HpySE526I ACGT 2 cut(s) 609, 1656
HspAI GCGC 1 cut(s) 1380
Ksp22I TGATCA 2 cut(s) 36, 310
KspAI GTTAAC 1 cut(s) 511
Kzo9I GATC 8 cut(s) 36, 70, 225, 310, 1220, 1553, 1738, 1786
LmnI GCTCC 5 cut(s) 527, 928, 1387, 1715, 1858
Lsp1109I GCAGC 7 cut(s) 68, 74, 77, 89, 401, 642, 810
LweI GCATC 7 cut(s) 9, 156, 464, 1598, 1901, 1960, 2125
MaeI CTAG 9 cut(s) 23, 555, 758, 1145, 1209, 1610, 1760, 1853, 1916
MaeII ACGT 2 cut(s) 609, 1656
MaeIII GTNAC 7 cut(s) 191, 244, 449, 488, 986, 2169, 2230
MalI GATC 8 cut(s) 38, 72, 227, 312, 1222, 1555, 1740, 1788
MboI GATC 8 cut(s) 36, 70, 225, 310, 1220, 1553, 1738, 1786
MboII GAAGA 9 cut(s) 344, 701, 878, 1081, 1152, 1384, 1594, 1926, 2216
MflI RGATCY 3 cut(s) 1220, 1553, 1786
MhlI GDGCHC 3 cut(s) 12, 524, 757
MlsI TGGCCA 1 cut(s) 50
MluNI TGGCCA 1 cut(s) 50
MlyI GAGTC 2 cut(s) 555, 1146
MmeI TCCRAC 3 cut(s) 255, 559, 1409
Mox20I TGGCCA 1 cut(s) 50
Mph1103I ATGCAT 1 cut(s) 479
MroXI GAANNNNTTC 2 cut(s) 711, 1797
MscI TGGCCA 1 cut(s) 50
MseI TTAA 6 cut(s) 510, 726, 738, 1106, 1407, 2270
MslI CAYNNNNRTG 5 cut(s) 44, 1037, 1097, 1287, 1443
Msp20I TGGCCA 1 cut(s) 50
MspA1I CMGCKG 5 cut(s) 59, 68, 801, 978, 2183
MspI CCGG 6 cut(s) 384, 471, 525, 853, 2139, 2217
MspR9I CCNGG 7 cut(s) 99, 385, 774, 864, 966, 1007, 1894
Mva1269I GAATGC 1 cut(s) 1955
MvaI CCWGG 6 cut(s) 99, 774, 864, 966, 1007, 1894
MvnI CGCG 1 cut(s) 1723
MwoI GCNNNNNNNGC 9 cut(s) 28, 56, 62, 65, 788, 864, 1920, 2024, 2139
NciI CCSGG 1 cut(s) 385
NcoI CCATGG 1 cut(s) 2120
NdeII GATC 8 cut(s) 36, 70, 225, 310, 1220, 1553, 1738, 1786
NlaIV GGNNCC 7 cut(s) 388, 443, 878, 924, 1555, 1788, 2215
NmuCI GTSAC 3 cut(s) 449, 488, 2169
NsiI ATGCAT 1 cut(s) 479
NspI RCATGY 5 cut(s) 340, 481, 1054, 1535, 2257
NspV TTCGAA 1 cut(s) 870
OliI CACNNNNGTG 1 cut(s) 1037
PaeI GCATGC 1 cut(s) 2257
PceI AGGCCT 1 cut(s) 1897
PciI ACATGT 1 cut(s) 336
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PctI GAATGC 1 cut(s) 1955
PdmI GAANNNNTTC 2 cut(s) 711, 1797
PfeI GAWTC 3 cut(s) 197, 1957, 2020
PfoI TCCNGGA 1 cut(s) 383
PleI GAGTC 2 cut(s) 555, 1146
PpsI GAGTC 2 cut(s) 555, 1146
PscI ACATGT 1 cut(s) 336
PshBI ATTAAT 1 cut(s) 1407
Psp6I CCWGG 6 cut(s) 97, 772, 862, 964, 1005, 1892
PspEI GGTNACC 1 cut(s) 2169
PspGI CCWGG 6 cut(s) 97, 772, 862, 964, 1005, 1892
PspN4I GGNNCC 7 cut(s) 388, 443, 878, 924, 1555, 1788, 2215
PspPI GGNCC 2 cut(s) 387, 813
PstI CTGCAG 1 cut(s) 1252
PstNI CAGNNNCTG 2 cut(s) 68, 1598
PsuI RGATCY 3 cut(s) 1220, 1553, 1786
PvuII CAGCTG 3 cut(s) 68, 801, 978
RsaI GTAC 5 cut(s) 341, 464, 1166, 1505, 2159
RsaNI GTAC 5 cut(s) 340, 463, 1165, 1504, 2158
RseI CAYNNNNRTG 5 cut(s) 44, 1037, 1097, 1287, 1443
SaqAI TTAA 6 cut(s) 510, 726, 738, 1106, 1407, 2270
Sau3AI GATC 8 cut(s) 36, 70, 225, 310, 1220, 1553, 1738, 1786
Sau96I GGNCC 2 cut(s) 387, 813
ScaI AGTACT 2 cut(s) 1166, 2159
SchI GAGTC 2 cut(s) 555, 1146
ScrFI CCNGG 7 cut(s) 99, 385, 774, 864, 966, 1007, 1894
SduI GDGCHC 3 cut(s) 12, 524, 757
SfaNI GCATC 7 cut(s) 9, 156, 464, 1598, 1901, 1960, 2125
SfcI CTRYAG 3 cut(s) 1248, 1734, 1834
SfuI TTCGAA 1 cut(s) 870
SinI GGWCC 2 cut(s) 387, 813
SmiMI CAYNNNNRTG 5 cut(s) 44, 1037, 1097, 1287, 1443
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
SpeI ACTAGT 2 cut(s) 1144, 1759
SphI GCATGC 1 cut(s) 2257
SseBI AGGCCT 1 cut(s) 1897
SsiI CCGC 8 cut(s) 44, 59, 201, 445, 558, 1182, 1856, 2183
SspI AATATT 1 cut(s) 2200
SspMI CTAG 9 cut(s) 23, 555, 758, 1145, 1209, 1610, 1760, 1853, 1916
StuI AGGCCT 1 cut(s) 1897
StyD4I CCNGG 7 cut(s) 97, 383, 772, 862, 964, 1005, 1892
StyI CCWWGG 2 cut(s) 1297, 2120
TaaI ACNGT 8 cut(s) 116, 244, 320, 503, 515, 577, 1321, 2005
TaiI ACGT 2 cut(s) 612, 1659
TaqI TCGA 7 cut(s) 73, 228, 819, 870, 1473, 1527, 2225
TaqII GACCGA 2 cut(s) 845, 1493
TatI WGTACW 4 cut(s) 339, 1164, 1503, 2157
TauI GCSGC 3 cut(s) 62, 1185, 2186
TfiI GAWTC 3 cut(s) 197, 1957, 2020
Tru1I TTAA 6 cut(s) 510, 726, 738, 1106, 1407, 2270
Tru9I TTAA 6 cut(s) 510, 726, 738, 1106, 1407, 2270
TscAI CASTG 3 cut(s) 119, 508, 709
TseFI GTSAC 3 cut(s) 449, 488, 2169
TseI GCWGC 7 cut(s) 56, 62, 65, 102, 414, 630, 798
Tsp45I GTSAC 3 cut(s) 449, 488, 2169
TspGWI ACGGA 1 cut(s) 2099
TspRI CASTG 3 cut(s) 119, 508, 709
VneI GTGCAC 1 cut(s) 8
VpaK11BI GGWCC 2 cut(s) 387, 813
VspI ATTAAT 1 cut(s) 1407
XapI RAATTY 4 cut(s) 958, 1062, 1962, 2259
XceI RCATGY 5 cut(s) 340, 481, 1054, 1535, 2257
XcmI CCANNNNNNNNNTGG 2 cut(s) 219, 962
XmnI GAANNNNTTC 2 cut(s) 711, 1797
XspI CTAG 9 cut(s) 23, 555, 758, 1145, 1209, 1610, 1760, 1853, 1916
ZrmI AGTACT 2 cut(s) 1166, 2159
Zsp2I ATGCAT 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.