Rroxscaffold_1G00060120

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000066
Physical Location & Seq
Forward (+)
82276581 .. 82279910
3330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00060120.1

Sequence Viewer

Length: 2235 bp
ATGAAACTCAAAGAAAAGTACTTTAAAGAGAATGGTGGCTTATTGTTACTACAGAAACTCTCTAATAATGGAGGCTCAGTGGAGACGACAAAAATATTCACCACAGAAGAACTAGAGAAGGCAACAAACAATTACCATGAGAGTAGAGTCCTTGGAGAAGGAGGCTATGGAACAGTTTATAAAGGGGTACTACTTGACAACAAAGTGGTTGCCATAAAGAAGTCCAAAGGTGGTACCTCGATCCAGAGTGAACAGTTTGTTAATGAGGTGGTTGTTCTTTCCCAAATCAACCACAGAAATGTAGTGAAGCTACTAGGTTGTTGTTTAGAGACAGCAGTACCTTTACTAGTATATGAGTTTGTCACCCATGGCACTTTATATGAGCACATTCATAGAAAAAGATCACTACTGTCATTCGAACTACGAATGAAGATAGCTGTTGAAACAGCAGGAGCACTAGCATACTTACACTCCTCTATATCCACGCCAATCATACATCGAGATGTGAAAGCAGCAAATATACTCCTAGATGATAATTATACAGCTAAAGTGTCGGACTTTGGAGCTTCACGGTTGGTTCCTTCAGGTCAAACTGAAATACAAACTTTAGTGCTCGGGACATTTGGATACCTAGACCCAGAATATTTGCAGTCAAACCAACTAACAGAAAAAAGTGATGTCTACAGCTTTGGAGTTGTCCTAGTGGAGCTACTAACAAGCAAAGTGGCATTTTCTAATGATAGATGCTTAGCAAGCATCTTTGTTTCTTCCATGGATGAAGATTGCTTAAAACAAATTCTTGATGATGCCATAGTGAATGAGGGAAACATTGAGACAGTAGAAAACGTGGCGAGTCTCGCAAAAAGATGTTTGAGGGTAAAAGGGGAGGAAAGGCCTACCATGAAAGAAGTTGCCATGGAGTTAGAGGGAATGGCAATTATGGCAAAGCATCCATGGGGAAAGAATGCCATGTTTTGTCCAGAGGAGAATGAGTACTTGCTTGGGTCACCTTCAGACAGTTATGTTATGGATGTTAGAGGAGGTGATCGCAATTCTAGTGGTTTAACTACTGGTATGACTACTGGGTATGACAGCATGCAAATCCAGATGTTGATGCCACGTGGTGTAAGTATAGGCTTATTGATTTTATTTGTGGGGATTTCGTGCATATGTTGCGGGATAAAGAGAAGACAATACACTAAACTCAAAGAAAAGTACTTCAAAGAGAATGGTGGCTTATTGTTACTACAACAACTTGCTAGTCATGGAGGCACTGTGGAGACAACAAAAATCTTTTCCACAGAAGAACTTGAGAAGGCAACAAACAATTACCACGAGAGTAGAATCCTTGGAGAAGTAGGCTATGGAACAGTTTACAAAGGAATACTACCAGATGACAAAGTGGTGGCCATAAAGAAGTCAAAAGGTGGTGCCCCAACCCAAAGTGATCAGTTTGTTAACGAGGTGATTGTTCTTTCTCAAATAAACCACAGAAATGTGGTGAAGCTACTAGGTTGTTGTTTAGAGACAGAAATAGCAGTTGAAACGGCAGAAGCACTGGCATACTTACACTCCTCAACTTCAAATCCAATCATACATCGAGATGTGAAAGCAGAAAATATACTCTTAGATGATGCTTATACAGAAAAAATATCAGACTTTGGAGCTTCAAGATTGATTCCATCAGGTCAAACTGAAATACAAACTTTAGTGCTAGGGACAGTTGGTTACCTAGACCCTGAATATCTGCAATCGAACCAACTAACAGAAAAGAGTGACGTCTACAGCTTTGGTGTTGTCCTAGTGGAGCTACTAACAAGCAAAATGGCACTTTCTAAGGACAGATGCTTAGCAAGCATCTTTATTTCTTCCATGGATGAAGATTGCTTGAATCAAATTCTTGATGATGACATTGTGAACGAGGGAAACATTGAGACAGTAAAGAATGTGGCCAGTCTTGCAAAAAGATGCTTGAGGGTAAAAGGGGAGGAAAGGCCTACCATGAAAGAAGTAGCAATGGAGCTAGAGGGAATGAGGATTACAGCAAAGCACCCATGGGGAACTAATGTTGGTTTCTGTCCAGAAGAAAATGAGTACTTGCTTGGGTCACTTGATTCAGATGCTTATGTTTCGGATGTTAGAGGAGGTGGTGGTAGTTCTAGTGGTTTAACTACTGGTACAGGGTATGACAGCATGCAAATACAAATGCTAATGCCTTATGGTGATGGACGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

744

Amino Acids

81.8

Weight (kDa)

5.26

Isoelectric Point (pI)

35.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 45 - 304 5.1e-51 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 48 - 308 6.1e-50 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 444 - 509 8.8e-07 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 447 - 511 1.1e-08 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 511 - 671 6.9e-30 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 511 - 675 2.2e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 180
AatII GACGTC 1 cut(s) 1782
Acc65I GGTACC 1 cut(s) 233
AccB1I GGYRCC 2 cut(s) 233, 1430
AccI GTMKAC 2 cut(s) 681, 1782
AciI CCGC 1 cut(s) 1176
AclWI GGATC 1 cut(s) 235
AcoI YGGCCR 2 cut(s) 1407, 1950
AcsI RAATTY 2 cut(s) 795, 1896
AcuI CTGAAG 2 cut(s) 567, 996
AcvI CACGTG 1 cut(s) 1121
AcyI GRCGYC 1 cut(s) 1779
AdeI CACNNNGTG 1 cut(s) 1124
AfaI GTAC 8 cut(s) 20, 189, 235, 339, 995, 1217, 2096, 2179
AgsI TTSAA 6 cut(s) 443, 1222, 1544, 1584, 1671, 1891
AhlI ACTAGT 1 cut(s) 346
Alw21I GWGCWC 3 cut(s) 387, 457, 615
Alw26I GTCTC 7 cut(s) 77, 323, 827, 860, 1274, 1520, 1928
AlwI GGATC 1 cut(s) 235
Ama87I CYCGRG 1 cut(s) 614
AoxI GGCC 4 cut(s) 893, 1407, 1950, 1994
ApeKI GCWGC 1 cut(s) 512
ApoI RAATTY 2 cut(s) 795, 1896
Asp718I GGTACC 1 cut(s) 233
AsuHPI GGTGA 7 cut(s) 91, 355, 999, 1055, 1477, 1513, 2234
AsuII TTCGAA 1 cut(s) 417
AvaI CYCGRG 1 cut(s) 614
BaeGI GKGCMC 1 cut(s) 1435
BaeI ACNNNNGTAYC 2 cut(s) 321, 354
BalI TGGCCA 2 cut(s) 1409, 1952
BanI GGYRCC 2 cut(s) 233, 1430
BauI CACGAG 1 cut(s) 1334
BbrPI CACGTG 1 cut(s) 1121
BbsI GAAGAC 1 cut(s) 1195
Bbv12I GWGCWC 3 cut(s) 387, 457, 615
BbvI GCAGC 1 cut(s) 524
BccI CCATC 2 cut(s) 1690, 2219
BceAI ACGGC 1 cut(s) 1563
BciVI GTATCC 1 cut(s) 620
BclI TGATCA 1 cut(s) 1447
BcoDI GTCTC 7 cut(s) 77, 323, 827, 860, 1274, 1520, 1928
BcuI ACTAGT 1 cut(s) 346
BfmI CTRYAG 3 cut(s) 50, 682, 1783
BfuI GTATCC 1 cut(s) 620
BisI GCNGC 1 cut(s) 513
BlpI GCTNAGC 2 cut(s) 748, 1849
BlsI GCNGC 1 cut(s) 514
BmcAI AGTACT 4 cut(s) 20, 995, 1217, 2096
BmeT110I CYCGRG 1 cut(s) 614
BmiI GGNNCC 3 cut(s) 235, 579, 1432
BmrI ACTGGG 1 cut(s) 1092
BmuI ACTGGG 1 cut(s) 1092
BpiI GAAGAC 1 cut(s) 1195
Bpu1102I GCTNAGC 2 cut(s) 748, 1849
Bpu14I TTCGAA 1 cut(s) 417
BpuEI CTTGAG 2 cut(s) 1331, 1993
BsaAI YACGTR 1 cut(s) 1121
BsaHI GRCGYC 1 cut(s) 1779
BsaJI CCNNGG 8 cut(s) 151, 367, 771, 915, 953, 1348, 1872, 2054
Bse1I ACTGG 5 cut(s) 1075, 1087, 1563, 1953, 2179
Bse3DI GCAATG 1 cut(s) 2022
BseDI CCNNGG 8 cut(s) 151, 367, 771, 915, 953, 1348, 1872, 2054
BseGI GGATG 5 cut(s) 781, 949, 1036, 1882, 2140
BseMI GCAATG 1 cut(s) 2022
BseMII CTCAG 1 cut(s) 90
BseNI ACTGG 5 cut(s) 1075, 1087, 1563, 1953, 2179
BseRI GAGGAG 5 cut(s) 463, 998, 1053, 1564, 2157
BseSI GKGCMC 1 cut(s) 1435
BseXI GCAGC 1 cut(s) 524
BshFI GGCC 4 cut(s) 895, 1409, 1952, 1996
BshNI GGYRCC 2 cut(s) 233, 1430
BsiHKAI GWGCWC 3 cut(s) 387, 457, 615
BsiHKCI CYCGRG 1 cut(s) 614
BslFI GGGAC 2 cut(s) 631, 1732
BsmAI GTCTC 7 cut(s) 77, 323, 827, 860, 1274, 1520, 1928
BsmBI CGTCTC 1 cut(s) 77
BsmFI GGGAC 2 cut(s) 631, 1732
BsmI GAATGC 1 cut(s) 970
BsnI GGCC 4 cut(s) 895, 1409, 1952, 1996
BsoBI CYCGRG 1 cut(s) 614
Bsp119I TTCGAA 1 cut(s) 417
Bsp1286I GDGCHC 4 cut(s) 387, 457, 615, 1435
Bsp143I GATC 4 cut(s) 240, 401, 1045, 1447
Bsp1720I GCTNAGC 2 cut(s) 748, 1849
Bsp19I CCATGG 6 cut(s) 367, 771, 915, 953, 1872, 2054
BspACI CCGC 1 cut(s) 1176
BspANI GGCC 4 cut(s) 895, 1409, 1952, 1996
BspCNI CTCAG 1 cut(s) 89
BspLI GGNNCC 3 cut(s) 235, 579, 1432
BspPI GGATC 1 cut(s) 235
BspT104I TTCGAA 1 cut(s) 417
BspT107I GGYRCC 2 cut(s) 233, 1430
BsrDI GCAATG 1 cut(s) 2022
BsrI ACTGG 5 cut(s) 1075, 1087, 1563, 1953, 2179
BssECI CCNNGG 8 cut(s) 151, 367, 771, 915, 953, 1348, 1872, 2054
BssMI GATC 4 cut(s) 240, 401, 1045, 1447
BssNI GRCGYC 1 cut(s) 1779
BssSI CACGAG 1 cut(s) 1334
BssT1I CCWWGG 8 cut(s) 151, 367, 771, 915, 953, 1348, 1872, 2054
Bst2BI CACGAG 1 cut(s) 1334
BstACI GRCGYC 1 cut(s) 1779
BstAPI GCANNNNNTGC 1 cut(s) 1173
BstBAI YACGTR 1 cut(s) 1121
BstBI TTCGAA 1 cut(s) 417
BstC8I GCNNGC 4 cut(s) 754, 1097, 1855, 2195
BstDEI CTNAG 5 cut(s) 76, 748, 1627, 1836, 1849
BstDSI CCRYGG 6 cut(s) 367, 771, 915, 953, 1872, 2054
BstEII GGTNACC 2 cut(s) 1005, 1727
BstF5I GGATG 5 cut(s) 781, 949, 1036, 1882, 2140
BstKTI GATC 4 cut(s) 243, 404, 1048, 1450
BstMAI GTCTC 7 cut(s) 77, 323, 827, 860, 1274, 1520, 1928
BstMBI GATC 4 cut(s) 240, 401, 1045, 1447
BstMWI GCNNNNNNNGC 6 cut(s) 753, 857, 941, 1173, 1854, 1958
BstNSI RCATGY 2 cut(s) 1099, 2197
BstPI GGTNACC 2 cut(s) 1005, 1727
BstSFI CTRYAG 3 cut(s) 50, 682, 1783
BstSLI GKGCMC 1 cut(s) 1435
BstV1I GCAGC 1 cut(s) 524
BstV2I GAAGAC 1 cut(s) 1195
BsuI GTATCC 1 cut(s) 620
BsuRI GGCC 4 cut(s) 895, 1409, 1952, 1996
BtgI CCRYGG 6 cut(s) 367, 771, 915, 953, 1872, 2054
BtsCI GGATG 5 cut(s) 781, 949, 1036, 1882, 2140
BtsIMutI CAGTG 3 cut(s) 84, 1272, 1556
Cac8I GCNNGC 4 cut(s) 754, 1097, 1855, 2195
Csp6I GTAC 8 cut(s) 19, 188, 234, 338, 994, 1216, 2095, 2178
CspCI CAANNNNNGTGG 4 cut(s) 705, 740, 1039, 1074
CviQI GTAC 8 cut(s) 19, 188, 234, 338, 994, 1216, 2095, 2178
DdeI CTNAG 5 cut(s) 76, 748, 1627, 1836, 1849
DpnI GATC 4 cut(s) 242, 403, 1047, 1449
DpnII GATC 4 cut(s) 240, 401, 1045, 1447
DraI TTTAAA 1 cut(s) 25
DraIII CACNNNGTG 1 cut(s) 1124
EaeI YGGCCR 2 cut(s) 1407, 1950
Eco130I CCWWGG 8 cut(s) 151, 367, 771, 915, 953, 1348, 1872, 2054
Eco147I AGGCCT 2 cut(s) 895, 1996
Eco57I CTGAAG 2 cut(s) 567, 996
Eco72I CACGTG 1 cut(s) 1121
Eco88I CYCGRG 1 cut(s) 614
Eco91I GGTNACC 2 cut(s) 1005, 1727
EcoO65I GGTNACC 2 cut(s) 1005, 1727
EcoT14I CCWWGG 8 cut(s) 151, 367, 771, 915, 953, 1348, 1872, 2054
ErhI CCWWGG 8 cut(s) 151, 367, 771, 915, 953, 1348, 1872, 2054
Esp3I CGTCTC 1 cut(s) 77
FaqI GGGAC 2 cut(s) 631, 1732
FauI CCCGC 1 cut(s) 1169
FauNDI CATATG 1 cut(s) 1169
FbaI TGATCA 1 cut(s) 1447
FblI GTMKAC 2 cut(s) 681, 1782
Fnu4HI GCNGC 1 cut(s) 513
FokI GGATG 5 cut(s) 788, 936, 1043, 1889, 2147
Fsp4HI GCNGC 1 cut(s) 513
GluI GCNGC 1 cut(s) 513
HaeIII GGCC 4 cut(s) 895, 1409, 1952, 1996
Hin1I GRCGYC 1 cut(s) 1779
HincII GTYRAC 1 cut(s) 1459
HindII GTYRAC 1 cut(s) 1459
HinfI GANTC 6 cut(s) 147, 853, 1344, 1678, 1891, 2114
HpaI GTTAAC 1 cut(s) 1459
HphI GGTGA 7 cut(s) 91, 355, 999, 1055, 1477, 1513, 2234
Hpy166II GTNNAC 6 cut(s) 251, 682, 1375, 1459, 1783, 1918
Hpy188I TCNGA 5 cut(s) 556, 1015, 1657, 2119, 2134
Hpy8I GTNNAC 6 cut(s) 251, 682, 1375, 1459, 1783, 1918
HpyAV CCTTC 5 cut(s) 112, 152, 591, 1020, 1309
HpyCH4IV ACGT 3 cut(s) 846, 1120, 1779
HpyCH4V TGCA 6 cut(s) 649, 1099, 1167, 1750, 1961, 2197
HpyF10VI GCNNNNNNNGC 6 cut(s) 753, 857, 941, 1173, 1854, 1958
HpyF3I CTNAG 5 cut(s) 76, 748, 1627, 1836, 1849
HpySE526I ACGT 3 cut(s) 846, 1120, 1779
Hsp92I GRCGYC 1 cut(s) 1779
KpnI GGTACC 1 cut(s) 237
Ksp22I TGATCA 1 cut(s) 1447
KspAI GTTAAC 1 cut(s) 1459
Kzo9I GATC 4 cut(s) 240, 401, 1045, 1447
LmnI GCTCC 6 cut(s) 452, 563, 706, 1664, 1807, 2020
Lsp1109I GCAGC 1 cut(s) 524
MaeII ACGT 3 cut(s) 846, 1120, 1779
MaeIII GTNAC 7 cut(s) 45, 361, 1005, 1242, 1727, 1775, 2106
MalI GATC 4 cut(s) 242, 403, 1047, 1449
MboI GATC 4 cut(s) 240, 401, 1045, 1447
MboII GAAGA 9 cut(s) 119, 442, 759, 791, 1200, 1316, 1860, 1892, 2096
MhlI GDGCHC 4 cut(s) 387, 457, 615, 1435
MlsI TGGCCA 2 cut(s) 1409, 1952
MluCI AATT 7 cut(s) 130, 535, 795, 936, 1051, 1327, 1896
MluNI TGGCCA 2 cut(s) 1409, 1952
MlyI GAGTC 2 cut(s) 156, 862
MmeI TCCRAC 1 cut(s) 534
Mox20I TGGCCA 2 cut(s) 1409, 1952
MscI TGGCCA 2 cut(s) 1409, 1952
MseI TTAA 6 cut(s) 24, 261, 788, 1064, 1458, 2168
MslI CAYNNNNRTG 4 cut(s) 297, 501, 1494, 1602
Msp20I TGGCCA 2 cut(s) 1409, 1952
Mva1269I GAATGC 1 cut(s) 970
MwoI GCNNNNNNNGC 6 cut(s) 753, 857, 941, 1173, 1854, 1958
NcoI CCATGG 6 cut(s) 367, 771, 915, 953, 1872, 2054
NdeI CATATG 1 cut(s) 1169
NdeII GATC 4 cut(s) 240, 401, 1045, 1447
NlaIV GGNNCC 3 cut(s) 235, 579, 1432
NmuCI GTSAC 4 cut(s) 361, 1005, 1775, 2106
NspI RCATGY 2 cut(s) 1099, 2197
NspV TTCGAA 1 cut(s) 417
PaeI GCATGC 2 cut(s) 1099, 2197
PceI AGGCCT 2 cut(s) 895, 1996
PctI GAATGC 1 cut(s) 970
PfeI GAWTC 4 cut(s) 1344, 1678, 1891, 2114
PkrI GCNGC 1 cut(s) 514
PleI GAGTC 2 cut(s) 155, 861
PmaCI CACGTG 1 cut(s) 1121
PmlI CACGTG 1 cut(s) 1121
PpsI GAGTC 2 cut(s) 155, 861
Ppu21I YACGTR 1 cut(s) 1121
PsiI TTATAA 1 cut(s) 180
PspCI CACGTG 1 cut(s) 1121
PspEI GGTNACC 2 cut(s) 1005, 1727
PspN4I GGNNCC 3 cut(s) 235, 579, 1432
RsaI GTAC 8 cut(s) 20, 189, 235, 339, 995, 1217, 2096, 2179
RsaNI GTAC 8 cut(s) 19, 188, 234, 338, 994, 1216, 2095, 2178
RseI CAYNNNNRTG 4 cut(s) 297, 501, 1494, 1602
SaqAI TTAA 6 cut(s) 24, 261, 788, 1064, 1458, 2168
SatI GCNGC 1 cut(s) 513
Sau3AI GATC 4 cut(s) 240, 401, 1045, 1447
ScaI AGTACT 4 cut(s) 20, 995, 1217, 2096
SchI GAGTC 2 cut(s) 156, 862
SduI GDGCHC 4 cut(s) 387, 457, 615, 1435
SfcI CTRYAG 3 cut(s) 50, 682, 1783
SfuI TTCGAA 1 cut(s) 417
SmiMI CAYNNNNRTG 4 cut(s) 297, 501, 1494, 1602
SmlI CTYRAG 2 cut(s) 1310, 1972
SmoI CTYRAG 2 cut(s) 1310, 1972
SpeI ACTAGT 1 cut(s) 346
SphI GCATGC 2 cut(s) 1099, 2197
Sse9I AATT 7 cut(s) 130, 535, 795, 936, 1051, 1327, 1896
SseBI AGGCCT 2 cut(s) 895, 1996
SsiI CCGC 1 cut(s) 1176
SspI AATATT 2 cut(s) 96, 644
StuI AGGCCT 2 cut(s) 895, 1996
StyI CCWWGG 8 cut(s) 151, 367, 771, 915, 953, 1348, 1872, 2054
TaiI ACGT 3 cut(s) 849, 1123, 1782
TaqI TCGA 5 cut(s) 239, 417, 499, 1600, 1754
TasI AATT 7 cut(s) 130, 535, 795, 936, 1051, 1327, 1896
TatI WGTACW 4 cut(s) 18, 993, 1215, 2094
TfiI GAWTC 4 cut(s) 1344, 1678, 1891, 2114
Tru1I TTAA 6 cut(s) 24, 261, 788, 1064, 1458, 2168
Tru9I TTAA 6 cut(s) 24, 261, 788, 1064, 1458, 2168
TscAI CASTG 3 cut(s) 84, 1279, 1563
TseFI GTSAC 4 cut(s) 361, 1005, 1775, 2106
TseI GCWGC 1 cut(s) 512
Tsp45I GTSAC 4 cut(s) 361, 1005, 1775, 2106
TspDTI ATGAA 7 cut(s) 17, 380, 443, 792, 917, 1893, 2018
TspRI CASTG 3 cut(s) 84, 1279, 1563
XapI RAATTY 2 cut(s) 795, 1896
XceI RCATGY 2 cut(s) 1099, 2197
XmiI GTMKAC 2 cut(s) 681, 1782
ZraI GACGTC 1 cut(s) 1780
ZrmI AGTACT 4 cut(s) 20, 995, 1217, 2096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.