FvH4_3g03320

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000112
Physical Location & Seq
Forward (+)
1786992 .. 1790349
3358 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g03320.t1

Sequence Viewer

Length: 2490 bp
ATGAAAATGAGGAAAAATATGCCATTGGTTCATTACTACACATTTGAGAGTGCAGAGAAAATGTTTTTGAAAGCTTTGCTTCTCTCCCTCCTCTTTCAGTTCTGCAGTTCCAGGGACACCATTAGAGGGAATGAAGACCAATTGAGGGATGTTGATGGAGACTATTTAGTGTCCAGAGAAAGGAAGTTTGAGTTGGGTTTCTTCGGCCCTGGAAATTCTAGCAACCGGTACGTTGGAATTTGGTATTCTGAGACTAGATTATCTCAAAAAATAGTGGTGTGGGTTGCAAACAGGAACAACCCCATTAATGATAGCTCTGGTGTGCTCACAATCAACAGGTATGGAGAATTTGTCCTTTATGCTCATAACATGAAGAGCACGCTTTGGTTTACTAATGTGACGCGGTCGGTCAAGAATGTTAACACTAGCACTTTATCAGCACAGCTTTTGGATACAGGAAATTTTGTTGTGTTCCAGGATGATAATAATGATATATTTATATGGCAGAGTTTTGATCATCCTACAGATACTCTACTTCCAGGTATGAAACTTGGGGTGAATTGGAAAACTGGGCTAGAATGGGTTTTAACATCTTGGAAGTCACATGATGACCCTGCAGCTGGGGAATATACACATAGGCTCTATCAAAATCAGACTTCTTTCCCCCAATTTTTTACATATAAAGGTTTGAGTAAGTATTGGCGAAGCGCTCCAGGGCCCGCTGCTAGTTTGGTCATTAATCAAGAGGAAACTTATTATTTCATGAATAAAATCAATGGAATTGCGAGAACAACTTTGAATGGTTCTGAGTTACAGTTCCTTTTATGGAGTGATGGAGACCGTCAATGGAAGCAAAGCTATGCTGAACCGAAATTACAGTGTGACTGGTACGGACATTGTGGTGCCAACAGCAAATGTTACCTTGACAATATCAGTCTGTTTGAGTGTGACTGTCTACCCGGTTTTGAACCAAAATCTATCAATGAGTGGAACCAGAATGATGGGTCAGATGGATGTGTGAGTAAACGAAAGGAAGTTTCTAAGTGTGGGAATGGAGATGGGTTTGTGCAGGTGACAAGCGTTAAATTTCCAGACACATCAATAGCAGCTCGACTGGAAGCACGTATAAGTAATGAAGTGTGTAGGCAGGAGTGCTTGGCAAATTGTTCTTGCAAAGCATATATGAGCCTTGAAAATGAAGAGGGTGTTGTTGATTGTTGGACATGGTATGATGATTTGATGGATATTATAGTGTACACAGAGGCTGGACGAGATCTATATGTTCGTGTGGATCAAATGGAATTAGCTGAGAGTGCTGGAAAATCCAAAGGTTTCTTTAAAAGGAGTATGCTTGCCATTTCTATAGTCTGTGCTCTGCTAGCATTGGCACTGATCATTATGTGTGCCTGTTGGTGGTGTAAGAAAAGAAGGAACACAACAGAAATTGTGGAGGCAGATGAACTTGAGGAAACTAAGAGGCACCCCGAATTGCAATTTTTCGATTTGAACACACTAATGGCAGCCACAGATAACTTCTCTCCTGTTAATGAACTCGGCCAAGGTGGTTTTGGCTCTGTTTATAAGGGTCAGTTACCAAACGAGCAGAAGGTTGCTGTGAAAAGGTTGTCTAAAACATCAGGACAAGGTACTGAAGAATTCAAAAACGAAGTTGCACTTATAGCAAGACTTCAACATCGCAATCTTGTAAAACTCTTGGGCTGTTGTATAAAGGGGGAAGAACGGATGTTAGTTCTAGAATACATGCCGAACAAAAGCTTGGACTCATTTCTCTTTGATCACACAAGACGGTCCTTCTTGGATTGGGAAAAGCGCTTTGAAATCATCAACGGGATTGCTCGTGGGATCCTGTATCTTCACCAAGACTCAAGATTGAGGATTATCCATAGAGATCTAAAGACTAGCAATGTTCTTTTAGATGCTGAAATGAACCCAAAAATTTCTGATTTTGGCATGGCAAGAATATTTGACGGGGACCAAGTTCAGGATAAAACCAATCGAGTTGTTGGAACATATGGCTACATGTCACCAGAGTATGCAGTGTTTGGCAGATTTTCTACTAAATCGGATGTTTTTAGTTTTGGGATCGTTTTGTTAGAGGTTGTGAGTGGTAAACGAAACAATAGTTTTTCTCAAGAGGATCCTTCCATGAACTTGATCAGACATGTTTGGAACCTGTGGAGAGAAGACAGAGTCTTAGAAATTGTGGATTCAATTCTCGAGTCGTATAATTCTGATGAGGTCATGAGATGCATTCAAGTTGCGCTCTTGTGCGTGCAAGAAGAATCAGAGGACCGGCCAGCCATGTCTACCATTGTTTTCATGTTGAGCGGCGAAACATCTCCTGCATCTCCTAACCAGCCTGCATATGTCTTCAGAAAAAACTCCATAACTGATGCTGATCCATCAGTTGTACCAACACCATTGTATTCTATGAACGACTTGACAATAACTGCTGAGGCAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

830

Amino Acids

94.63

Weight (kDa)

5.51

Isoelectric Point (pI)

40.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 92 - 200 3.6e-35 D-mannose binding lectin
S_locus_glycop PF00954 250 - 326 1.1e-12 S-locus glycoprotein domain
PAN_2 PF08276 349 - 416 1.9e-15 PAN-like domain
Pkinase PF00069 515 - 777 4.6e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 516 - 780 3.2e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1581
AarI CACCTGC 1 cut(s) 1060
Acc36I ACCTGC 1 cut(s) 1060
AccB1I GGYRCC 2 cut(s) 902, 1479
AccBSI CCGCTC 1 cut(s) 2349
AccI GTMKAC 2 cut(s) 955, 2327
AccII CGCG 1 cut(s) 403
AciI CCGC 3 cut(s) 403, 720, 2349
AclWI GGATC 7 cut(s) 1299, 1858, 1871, 2111, 2153, 2166, 2414
AcoI YGGCCR 2 cut(s) 1555, 2315
AcsI RAATTY 7 cut(s) 214, 237, 347, 460, 1085, 1655, 1956
AcuI CTGAAG 2 cut(s) 1671, 2377
AfaI GTAC 5 cut(s) 230, 890, 1256, 1648, 2433
AfeI AGCGCT 2 cut(s) 709, 1832
AfiI CCNNNNNNNGG 6 cut(s) 126, 145, 180, 620, 1413, 2002
AflIII ACRYGT 2 cut(s) 2040, 2182
AgeI ACCGGT 1 cut(s) 225
AjnI CCWGG 5 cut(s) 110, 208, 474, 538, 712
AjuI GAANNNNNNNTTGG 4 cut(s) 176, 208, 1760, 1792
AluBI AGCT 9 cut(s) 74, 315, 445, 620, 858, 1109, 1307, 1776, 2483
AluI AGCT 9 cut(s) 74, 315, 445, 620, 858, 1109, 1307, 1776, 2483
Alw21I GWGCWC 3 cut(s) 327, 380, 1375
Alw26I GTCTC 3 cut(s) 153, 245, 831
AlwI GGATC 7 cut(s) 1299, 1858, 1871, 2111, 2153, 2166, 2414
AlwNI CAGNNNCTG 1 cut(s) 1265
Ama87I CYCGRG 1 cut(s) 2237
Aor51HI AGCGCT 2 cut(s) 709, 1832
AoxI GGCC 4 cut(s) 205, 716, 1555, 2315
ApaI GGGCCC 1 cut(s) 720
ApeKI GCWGC 5 cut(s) 617, 722, 1106, 1520, 2480
ApoI RAATTY 7 cut(s) 214, 237, 347, 460, 1085, 1655, 1956
AseI ATTAAT 2 cut(s) 306, 738
AsiGI ACCGGT 1 cut(s) 225
AspLEI GCGC 3 cut(s) 710, 1833, 2284
AspS9I GGNCC 6 cut(s) 206, 716, 717, 1809, 1993, 2311
AsuC2I CCSGG 1 cut(s) 960
AsuHPI GGTGA 4 cut(s) 568, 1084, 1868, 2037
AsuNHI GCTAGC 1 cut(s) 1378
AvaI CYCGRG 1 cut(s) 2237
AvaII GGWCC 3 cut(s) 1809, 1993, 2311
BaeGI GKGCMC 1 cut(s) 720
BamHI GGATCC 2 cut(s) 1863, 2158
BanI GGYRCC 2 cut(s) 902, 1479
BanII GRGCYC 1 cut(s) 720
BauI CACGAG 1 cut(s) 1857
BbsI GAAGAC 3 cut(s) 141, 2211, 2383
Bbv12I GWGCWC 3 cut(s) 327, 380, 1375
BbvCI CCTCAGC 1 cut(s) 2475
BbvI GCAGC 4 cut(s) 629, 709, 1118, 1532
BccI CCATC 7 cut(s) 149, 827, 995, 1004, 1052, 1234, 2431
BciT130I CCWGG 5 cut(s) 112, 210, 476, 540, 714
BciVI GTATCC 1 cut(s) 445
BclI TGATCA 4 cut(s) 514, 1392, 1795, 2175
BcnI CCSGG 1 cut(s) 960
BcoDI GTCTC 3 cut(s) 153, 245, 831
BfaI CTAG 8 cut(s) 219, 255, 426, 575, 726, 1379, 1754, 1920
BfmI CTRYAG 4 cut(s) 103, 522, 615, 1362
BfoI RGCGCY 2 cut(s) 711, 1834
BfuAI ACCTGC 1 cut(s) 1060
BfuI GTATCC 1 cut(s) 445
BglII AGATCT 2 cut(s) 1273, 1909
BisI GCNGC 6 cut(s) 618, 723, 1107, 1521, 2350, 2481
BlsI GCNGC 6 cut(s) 619, 724, 1108, 1522, 2351, 2482
Bme1390I CCNGG 6 cut(s) 112, 210, 476, 540, 714, 960
Bme18I GGWCC 3 cut(s) 1809, 1993, 2311
BmeT110I CYCGRG 1 cut(s) 2237
BmgT120I GGNCC 6 cut(s) 206, 716, 717, 1809, 1993, 2311
BmiI GGNNCC 8 cut(s) 718, 904, 992, 1481, 1865, 1994, 2160, 2192
BmrFI CCNGG 6 cut(s) 112, 210, 476, 540, 714, 960
BmrI ACTGGG 1 cut(s) 579
BmsI GCATC 4 cut(s) 1927, 2258, 2375, 2404
BmtI GCTAGC 1 cut(s) 1382
BmuI ACTGGG 1 cut(s) 579
BpiI GAAGAC 3 cut(s) 141, 2211, 2383
BpmI CTGGAG 1 cut(s) 696
Bpu10I CCTNAGC 1 cut(s) 2475
BpuEI CTTGAG 3 cut(s) 1484, 1870, 2136
BpuMI CCSGG 1 cut(s) 960
BsaAI YACGTR 1 cut(s) 1124
BsaBI GATNNNNATC 1 cut(s) 2418
BsaI GGTCTC 1 cut(s) 831
BsaJI CCNNGG 4 cut(s) 111, 208, 713, 1558
BsaWI WCCGGW 1 cut(s) 225
BsaXI ACNNNNNCTCC 2 cut(s) 336, 366
Bsc4I CCNNNNNNNGG 6 cut(s) 126, 145, 180, 620, 1413, 2002
Bse118I RCCGGY 2 cut(s) 225, 2313
Bse1I ACTGG 3 cut(s) 574, 890, 1119
Bse3DI GCAATG 1 cut(s) 1930
Bse8I GATNNNNATC 1 cut(s) 2418
BseBI CCWGG 5 cut(s) 112, 210, 476, 540, 714
BseDI CCNNGG 4 cut(s) 111, 208, 713, 1558
BseGI GGATG 6 cut(s) 154, 484, 517, 1019, 1749, 2092
BseJI GATNNNNATC 1 cut(s) 2418
BseLI CCNNNNNNNGG 6 cut(s) 126, 145, 180, 620, 1413, 2002
BseMI GCAATG 1 cut(s) 1930
BseMII CTCAG 4 cut(s) 240, 798, 1299, 2466
BseNI ACTGG 3 cut(s) 574, 890, 1119
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 720
BseXI GCAGC 4 cut(s) 629, 709, 1118, 1532
BseYI CCCAGC 1 cut(s) 620
BsgI GTGCAG 2 cut(s) 72, 1088
Bsh1236I CGCG 1 cut(s) 403
Bsh1285I CGRYCG 1 cut(s) 407
BshFI GGCC 4 cut(s) 207, 718, 1557, 2317
BshNI GGYRCC 2 cut(s) 902, 1479
BshTI ACCGGT 1 cut(s) 225
BsiEI CGRYCG 1 cut(s) 407
BsiHKAI GWGCWC 3 cut(s) 327, 380, 1375
BsiHKCI CYCGRG 1 cut(s) 2237
BsiSI CCGG 3 cut(s) 226, 960, 2314
BslFI GGGAC 2 cut(s) 128, 2006
BslI CCNNNNNNNGG 6 cut(s) 126, 145, 180, 620, 1413, 2002
BsmAI GTCTC 3 cut(s) 153, 245, 831
BsmFI GGGAC 2 cut(s) 128, 2006
BsmI GAATGC 1 cut(s) 2271
BsnI GGCC 4 cut(s) 207, 718, 1557, 2317
Bso31I GGTCTC 1 cut(s) 831
BsoBI CYCGRG 1 cut(s) 2237
Bsp120I GGGCCC 1 cut(s) 716
Bsp1286I GDGCHC 4 cut(s) 327, 380, 720, 1375
Bsp1407I TGTACA 1 cut(s) 1254
BspACI CCGC 3 cut(s) 403, 720, 2349
BspANI GGCC 4 cut(s) 207, 718, 1557, 2317
BspCNI CTCAG 4 cut(s) 241, 799, 1300, 2467
BspFNI CGCG 1 cut(s) 403
BspHI TCATGA 2 cut(s) 762, 2262
BspLI GGNNCC 8 cut(s) 718, 904, 992, 1481, 1865, 1994, 2160, 2192
BspMAI CTGCAG 2 cut(s) 107, 619
BspMI ACCTGC 1 cut(s) 1060
BspOI GCTAGC 1 cut(s) 1382
BspPI GGATC 7 cut(s) 1299, 1858, 1871, 2111, 2153, 2166, 2414
BspQI GCTCTTC 1 cut(s) 368
BspT107I GGYRCC 2 cut(s) 902, 1479
BspTNI GGTCTC 1 cut(s) 831
BsrBI CCGCTC 1 cut(s) 2349
BsrDI GCAATG 1 cut(s) 1930
BsrFI RCCGGY 2 cut(s) 225, 2313
BsrGI TGTACA 1 cut(s) 1254
BsrI ACTGG 3 cut(s) 574, 890, 1119
BssAI RCCGGY 2 cut(s) 225, 2313
BssECI CCNNGG 4 cut(s) 111, 208, 713, 1558
BssSI CACGAG 1 cut(s) 1857
BssT1I CCWWGG 1 cut(s) 1558
Bst2BI CACGAG 1 cut(s) 1857
Bst2UI CCWGG 5 cut(s) 112, 210, 476, 540, 714
Bst4CI ACNGT 5 cut(s) 816, 842, 879, 953, 1809
Bst6I CTCTTC 2 cut(s) 368, 1194
BstAUI TGTACA 1 cut(s) 1254
BstBAI YACGTR 1 cut(s) 1124
BstC8I GCNNGC 7 cut(s) 380, 720, 1353, 1380, 2294, 2319, 2382
BstDEI CTNAG 7 cut(s) 249, 807, 1041, 1308, 1473, 2215, 2475
BstF5I GGATG 6 cut(s) 154, 484, 517, 1019, 1749, 2092
BstFNI CGCG 1 cut(s) 403
BstH2I RGCGCY 2 cut(s) 711, 1834
BstHHI GCGC 3 cut(s) 710, 1833, 2284
BstMAI GTCTC 3 cut(s) 153, 245, 831
BstMCI CGRYCG 1 cut(s) 407
BstMWI GCNNNNNNNGC 4 cut(s) 1313, 1379, 1679, 2480
BstNI CCWGG 5 cut(s) 112, 210, 476, 540, 714
BstNSI RCATGY 3 cut(s) 1765, 2044, 2186
BstSCI CCNGG 6 cut(s) 110, 208, 474, 538, 712, 958
BstSFI CTRYAG 4 cut(s) 103, 522, 615, 1362
BstSLI GKGCMC 1 cut(s) 720
BstUI CGCG 1 cut(s) 403
BstV1I GCAGC 4 cut(s) 629, 709, 1118, 1532
BstV2I GAAGAC 3 cut(s) 141, 2211, 2383
BstX2I RGATCY 4 cut(s) 1273, 1863, 1909, 2158
BstXI CCANNNNNNTGG 1 cut(s) 1001
BstYI RGATCY 4 cut(s) 1273, 1863, 1909, 2158
BsuI GTATCC 1 cut(s) 445
BsuRI GGCC 4 cut(s) 207, 718, 1557, 2317
BtgZI GCGATG 1 cut(s) 1679
BtsCI GGATG 6 cut(s) 154, 484, 517, 1019, 1749, 2092
BtsI GCAGTG 1 cut(s) 2064
BtsIMutI CAGTG 3 cut(s) 884, 1388, 2064
BveI ACCTGC 1 cut(s) 1060
Cac8I GCNNGC 7 cut(s) 380, 720, 1353, 1380, 2294, 2319, 2382
CaiI CAGNNNCTG 1 cut(s) 1265
CciI TCATGA 2 cut(s) 762, 2262
CfoI GCGC 3 cut(s) 710, 1833, 2284
Cfr10I RCCGGY 2 cut(s) 225, 2313
Cfr13I GGNCC 6 cut(s) 206, 716, 717, 1809, 1993, 2311
CseI GACGC 1 cut(s) 409
Csp6I GTAC 5 cut(s) 229, 889, 1255, 1647, 2432
CspAI ACCGGT 1 cut(s) 225
CviQI GTAC 5 cut(s) 229, 889, 1255, 1647, 2432
DdeI CTNAG 7 cut(s) 249, 807, 1041, 1308, 1473, 2215, 2475
DraI TTTAAA 1 cut(s) 1339
EaeI YGGCCR 2 cut(s) 1555, 2315
Eam1104I CTCTTC 2 cut(s) 368, 1194
EarI CTCTTC 2 cut(s) 368, 1194
Eco130I CCWWGG 1 cut(s) 1558
Eco24I GRGCYC 1 cut(s) 720
Eco31I GGTCTC 1 cut(s) 831
Eco47I GGWCC 3 cut(s) 1809, 1993, 2311
Eco47III AGCGCT 2 cut(s) 709, 1832
Eco57I CTGAAG 2 cut(s) 1671, 2377
Eco88I CYCGRG 1 cut(s) 2237
EcoO109I RGGNCCY 1 cut(s) 716
EcoRI GAATTC 1 cut(s) 1655
EcoRII CCWGG 5 cut(s) 110, 208, 474, 538, 712
EcoT14I CCWWGG 1 cut(s) 1558
EcoT22I ATGCAT 1 cut(s) 2273
EcoT38I GRGCYC 1 cut(s) 720
ErhI CCWWGG 1 cut(s) 1558
FalI AAGNNNNNCTT 4 cut(s) 63, 95, 1659, 1691
FaqI GGGAC 2 cut(s) 128, 2006
FauI CCCGC 1 cut(s) 727
FauNDI CATATG 2 cut(s) 2032, 2386
FbaI TGATCA 4 cut(s) 514, 1392, 1795, 2175
FblI GTMKAC 2 cut(s) 955, 2327
Fnu4HI GCNGC 6 cut(s) 618, 723, 1107, 1521, 2350, 2481
FokI GGATG 6 cut(s) 161, 491, 504, 1026, 1756, 2099
FriOI GRGCYC 1 cut(s) 720
Fsp4HI GCNGC 6 cut(s) 618, 723, 1107, 1521, 2350, 2481
FspBI CTAG 8 cut(s) 219, 255, 426, 575, 726, 1379, 1754, 1920
GlaI GCGC 3 cut(s) 709, 1832, 2283
GluI GCNGC 6 cut(s) 618, 723, 1107, 1521, 2350, 2481
GsaI CCCAGC 1 cut(s) 624
GsuI CTGGAG 1 cut(s) 696
HaeII RGCGCY 2 cut(s) 711, 1834
HaeIII GGCC 4 cut(s) 207, 718, 1557, 2317
HapII CCGG 3 cut(s) 226, 960, 2314
HgaI GACGC 1 cut(s) 409
HhaI GCGC 3 cut(s) 710, 1833, 2284
Hin6I GCGC 3 cut(s) 708, 1831, 2282
HinP1I GCGC 3 cut(s) 708, 1831, 2282
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HindIII AAGCTT 2 cut(s) 72, 1774
HinfI GANTC 6 cut(s) 1781, 1883, 2211, 2228, 2240, 2303
HpaI GTTAAC 1 cut(s) 421
HpaII CCGG 3 cut(s) 226, 960, 2314
HphI GGTGA 4 cut(s) 568, 1084, 1868, 2037
Hpy166II GTNNAC 8 cut(s) 390, 421, 956, 1025, 1255, 1257, 2132, 2328
Hpy8I GTNNAC 8 cut(s) 390, 421, 956, 1025, 1255, 1257, 2132, 2328
HpyAV CCTTC 4 cut(s) 1422, 1600, 1822, 2172
HpyCH4III ACNGT 5 cut(s) 816, 842, 879, 953, 1809
HpyCH4IV ACGT 2 cut(s) 231, 1123
HpyF10VI GCNNNNNNNGC 4 cut(s) 1313, 1379, 1679, 2480
HpyF3I CTNAG 7 cut(s) 249, 807, 1041, 1308, 1473, 2215, 2475
HpySE526I ACGT 2 cut(s) 231, 1123
HspAI GCGC 3 cut(s) 708, 1831, 2282
Ksp22I TGATCA 4 cut(s) 514, 1392, 1795, 2175
KspAI GTTAAC 1 cut(s) 421
LguI GCTCTTC 1 cut(s) 368
LmnI GCTCC 1 cut(s) 715
Lsp1109I GCAGC 4 cut(s) 629, 709, 1118, 1532
LweI GCATC 4 cut(s) 1927, 2258, 2375, 2404
MaeI CTAG 8 cut(s) 219, 255, 426, 575, 726, 1379, 1754, 1920
MaeII ACGT 2 cut(s) 231, 1123
MaeIII GTNAC 9 cut(s) 397, 600, 810, 881, 917, 947, 1072, 1590, 2043
MbiI CCGCTC 1 cut(s) 2349
MfeI CAATTG 1 cut(s) 140
MflI RGATCY 4 cut(s) 1273, 1863, 1909, 2158
MhlI GDGCHC 4 cut(s) 327, 380, 720, 1375
MlyI GAGTC 4 cut(s) 1775, 1877, 2220, 2249
MmeI TCCRAC 3 cut(s) 214, 1199, 2005
Mph1103I ATGCAT 1 cut(s) 2273
MseI TTAA 7 cut(s) 306, 420, 587, 738, 1083, 1338, 1545
MslI CAYNNNNRTG 2 cut(s) 900, 1514
MspA1I CMGCKG 2 cut(s) 620, 722
MspI CCGG 3 cut(s) 226, 960, 2314
MspR9I CCNGG 6 cut(s) 112, 210, 476, 540, 714, 960
MunI CAATTG 1 cut(s) 140
Mva1269I GAATGC 1 cut(s) 2271
MvaI CCWGG 5 cut(s) 112, 210, 476, 540, 714
MvnI CGCG 1 cut(s) 403
MwoI GCNNNNNNNGC 4 cut(s) 1313, 1379, 1679, 2480
NciI CCSGG 1 cut(s) 960
NdeI CATATG 2 cut(s) 2032, 2386
NheI GCTAGC 1 cut(s) 1378
NlaIV GGNNCC 8 cut(s) 718, 904, 992, 1481, 1865, 1994, 2160, 2192
NmeAIII GCCGAG 1 cut(s) 1533
NmuCI GTSAC 6 cut(s) 397, 600, 881, 947, 1072, 2043
NsiI ATGCAT 1 cut(s) 2273
NspI RCATGY 3 cut(s) 1765, 2044, 2186
PaeR7I CTCGAG 1 cut(s) 2237
PagI TCATGA 2 cut(s) 762, 2262
PaqCI CACCTGC 1 cut(s) 1060
PciI ACATGT 2 cut(s) 2040, 2182
PciSI GCTCTTC 1 cut(s) 368
PctI GAATGC 1 cut(s) 2271
PfeI GAWTC 2 cut(s) 2228, 2303
PflFI GACNNNGTC 2 cut(s) 403, 2210
PfoI TCCNGGA 1 cut(s) 474
PinAI ACCGGT 1 cut(s) 225
PkrI GCNGC 6 cut(s) 619, 724, 1108, 1522, 2351, 2482
PleI GAGTC 4 cut(s) 1775, 1877, 2219, 2248
PpsI GAGTC 4 cut(s) 1775, 1877, 2219, 2248
Ppu21I YACGTR 1 cut(s) 1124
PscI ACATGT 2 cut(s) 2040, 2182
PshBI ATTAAT 2 cut(s) 306, 738
PsiI TTATAA 1 cut(s) 1581
Psp6I CCWGG 5 cut(s) 110, 208, 474, 538, 712
PspFI CCCAGC 1 cut(s) 620
PspGI CCWGG 5 cut(s) 110, 208, 474, 538, 712
PspN4I GGNNCC 8 cut(s) 718, 904, 992, 1481, 1865, 1994, 2160, 2192
PspOMI GGGCCC 1 cut(s) 716
PspPI GGNCC 6 cut(s) 206, 716, 717, 1809, 1993, 2311
PsrI GAACNNNNNNTAC 2 cut(s) 858, 890
PstI CTGCAG 2 cut(s) 107, 619
PstNI CAGNNNCTG 1 cut(s) 1265
PsuI RGATCY 4 cut(s) 1273, 1863, 1909, 2158
PsyI GACNNNGTC 2 cut(s) 403, 2210
PvuII CAGCTG 1 cut(s) 620
RsaI GTAC 5 cut(s) 230, 890, 1256, 1648, 2433
RsaNI GTAC 5 cut(s) 229, 889, 1255, 1647, 2432
RseI CAYNNNNRTG 2 cut(s) 900, 1514
SapI GCTCTTC 1 cut(s) 368
SaqAI TTAA 7 cut(s) 306, 420, 587, 738, 1083, 1338, 1545
SatI GCNGC 6 cut(s) 618, 723, 1107, 1521, 2350, 2481
Sau96I GGNCC 6 cut(s) 206, 716, 717, 1809, 1993, 2311
SchI GAGTC 4 cut(s) 1775, 1877, 2220, 2249
ScrFI CCNGG 6 cut(s) 112, 210, 476, 540, 714, 960
SduI GDGCHC 4 cut(s) 327, 380, 720, 1375
SfaNI GCATC 4 cut(s) 1927, 2258, 2375, 2404
SfcI CTRYAG 4 cut(s) 103, 522, 615, 1362
Sfr274I CTCGAG 1 cut(s) 2237
SinI GGWCC 3 cut(s) 1809, 1993, 2311
SlaI CTCGAG 1 cut(s) 2237
SmiMI CAYNNNNRTG 2 cut(s) 900, 1514
SmlI CTYRAG 4 cut(s) 1463, 1885, 2151, 2237
SmoI CTYRAG 4 cut(s) 1463, 1885, 2151, 2237
SsiI CCGC 3 cut(s) 403, 720, 2349
SspI AATATT 1 cut(s) 1983
SspMI CTAG 8 cut(s) 219, 255, 426, 575, 726, 1379, 1754, 1920
StyD4I CCNGG 6 cut(s) 110, 208, 474, 538, 712, 958
StyI CCWWGG 1 cut(s) 1558
TaaI ACNGT 5 cut(s) 816, 842, 879, 953, 1809
TaiI ACGT 2 cut(s) 234, 1126
TaqI TCGA 5 cut(s) 1111, 1500, 2017, 2238, 2485
TaqII GACCGA 1 cut(s) 397
TatI WGTACW 1 cut(s) 1254
TauI GCSGC 1 cut(s) 2352
TfiI GAWTC 2 cut(s) 2228, 2303
Tru1I TTAA 7 cut(s) 306, 420, 587, 738, 1083, 1338, 1545
Tru9I TTAA 7 cut(s) 306, 420, 587, 738, 1083, 1338, 1545
TscAI CASTG 3 cut(s) 884, 1395, 2064
TseFI GTSAC 6 cut(s) 397, 600, 881, 947, 1072, 2043
TseI GCWGC 5 cut(s) 617, 722, 1106, 1520, 2480
Tsp45I GTSAC 6 cut(s) 397, 600, 881, 947, 1072, 2043
TspGWI ACGGA 2 cut(s) 906, 1756
TspRI CASTG 3 cut(s) 884, 1395, 2064
Tth111I GACNNNGTC 2 cut(s) 403, 2210
VpaK11BI GGWCC 3 cut(s) 1809, 1993, 2311
VspI ATTAAT 2 cut(s) 306, 738
XapI RAATTY 7 cut(s) 214, 237, 347, 460, 1085, 1655, 1956
XbaI TCTAGA 1 cut(s) 1753
XceI RCATGY 3 cut(s) 1765, 2044, 2186
XcmI CCANNNNNNNNNTGG 1 cut(s) 1565
XhoI CTCGAG 1 cut(s) 2237
XmiI GTMKAC 2 cut(s) 955, 2327
XspI CTAG 8 cut(s) 219, 255, 426, 575, 726, 1379, 1754, 1920
Zsp2I ATGCAT 1 cut(s) 2273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.