Rh3DG233500

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000112
Physical Location & Seq
Forward (+)
22188306 .. 22188752
447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG233500.1

Sequence Viewer

Length: 447 bp
ATGAAAAAGGACCTCTGGTCATTTTTTATATTCACACATAGCCAGATTGCAAATGCCCTGAAAATGCTGTTGAAATCTCTGCTTCTCTTCCTCCTCTTCCAAGTTTGCACTTCCAGGGACACCTTGAGATCGAACGAAGAACTGAAGGATGATGATGGTGGAATTCTGGTGTCCAAAGAAAGCAGCTTTGAGTTGGGTTTCTTCAGCCCTGGAAATTCTAGCAACCGCTATGTTGGGATTTGGTATTCTCAATCTAAAGTATCTGAAAAAACAGTGGTGTGGATCGCAAACAGAGACAACCCCATCAATGATACCTCTAGAGTTTTCACCATAAACAGGTATGGAGACCTAGTTCTTTATGCTTATAACATGGAGACCAGTCCTATTCAGTCAAAAACACTGGCACCTTATCTGCATATGTACAGCTGTTGGATACAGGAAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

148

Amino Acids

17.12

Weight (kDa)

6.72

Isoelectric Point (pI)

47.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 91 - 124 2.5e-08 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 366
AccB1I GGYRCC 1 cut(s) 403
AciI CCGC 1 cut(s) 226
AclWI GGATC 1 cut(s) 290
AcsI RAATTY 2 cut(s) 162, 214
AcuI CTGAAG 2 cut(s) 164, 187
AfaI GTAC 1 cut(s) 422
AfiI CCNNNNNNNGG 1 cut(s) 336
AgsI TTSAA 1 cut(s) 73
AhdI GACNNNNNGTC 1 cut(s) 16
AjnI CCWGG 2 cut(s) 113, 208
AloI GAACNNNNNNTCC 2 cut(s) 336, 368
AluBI AGCT 2 cut(s) 186, 426
AluI AGCT 2 cut(s) 186, 426
Alw26I GTCTC 3 cut(s) 288, 339, 368
AlwI GGATC 1 cut(s) 290
ApeKI GCWGC 1 cut(s) 183
ApoI RAATTY 2 cut(s) 162, 214
AspS9I GGNCC 1 cut(s) 10
AsuHPI GGTGA 1 cut(s) 319
AvaII GGWCC 1 cut(s) 10
BanI GGYRCC 1 cut(s) 403
BbvI GCAGC 1 cut(s) 195
BccI CCATC 2 cut(s) 149, 311
BciT130I CCWGG 2 cut(s) 115, 210
BciVI GTATCC 1 cut(s) 426
BcoDI GTCTC 3 cut(s) 288, 339, 368
BfaI CTAG 3 cut(s) 219, 318, 350
BfuI GTATCC 1 cut(s) 426
BisI GCNGC 1 cut(s) 184
BlsI GCNGC 1 cut(s) 185
Bme1390I CCNGG 2 cut(s) 115, 210
Bme18I GGWCC 1 cut(s) 10
BmeRI GACNNNNNGTC 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 10
BmiI GGNNCC 1 cut(s) 405
BmrFI CCNGG 2 cut(s) 115, 210
BpuEI CTTGAG 1 cut(s) 145
BsaI GGTCTC 2 cut(s) 339, 368
BsaJI CCNNGG 2 cut(s) 114, 208
BsaXI ACNNNNNCTCC 2 cut(s) 336, 366
Bsc4I CCNNNNNNNGG 1 cut(s) 336
Bse1I ACTGG 2 cut(s) 378, 405
BseBI CCWGG 2 cut(s) 115, 210
BseDI CCNNGG 2 cut(s) 114, 208
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 1 cut(s) 336
BseNI ACTGG 2 cut(s) 378, 405
BseRI GAGGAG 1 cut(s) 83
BseXI GCAGC 1 cut(s) 195
BshNI GGYRCC 1 cut(s) 403
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 1 cut(s) 336
BsmAI GTCTC 3 cut(s) 288, 339, 368
BsmFI GGGAC 1 cut(s) 131
Bso31I GGTCTC 2 cut(s) 339, 368
Bsp1407I TGTACA 1 cut(s) 420
Bsp143I GATC 2 cut(s) 128, 282
BspACI CCGC 1 cut(s) 226
BspLI GGNNCC 1 cut(s) 405
BspPI GGATC 1 cut(s) 290
BspT107I GGYRCC 1 cut(s) 403
BspTNI GGTCTC 2 cut(s) 339, 368
BsrGI TGTACA 1 cut(s) 420
BsrI ACTGG 2 cut(s) 378, 405
BssECI CCNNGG 2 cut(s) 114, 208
BssMI GATC 2 cut(s) 128, 282
Bst2UI CCWGG 2 cut(s) 115, 210
Bst4CI ACNGT 1 cut(s) 274
Bst6I CTCTTC 2 cut(s) 92, 101
BstAUI TGTACA 1 cut(s) 420
BstDEI CTNAG 1 cut(s) 444
BstF5I GGATG 1 cut(s) 154
BstKTI GATC 2 cut(s) 131, 285
BstMAI GTCTC 3 cut(s) 288, 339, 368
BstMBI GATC 2 cut(s) 128, 282
BstNI CCWGG 2 cut(s) 115, 210
BstSCI CCNGG 2 cut(s) 113, 208
BstV1I GCAGC 1 cut(s) 195
BsuI GTATCC 1 cut(s) 426
BtsCI GGATG 1 cut(s) 154
BtsIMutI CAGTG 2 cut(s) 279, 398
Cfr13I GGNCC 1 cut(s) 10
Csp6I GTAC 1 cut(s) 421
CviAII CATG 1 cut(s) 370
CviJI RGCY 4 cut(s) 42, 186, 207, 426
CviKI_1 RGCY 4 cut(s) 42, 186, 207, 426
CviQI GTAC 1 cut(s) 421
DdeI CTNAG 1 cut(s) 444
DpnI GATC 2 cut(s) 130, 284
DpnII GATC 2 cut(s) 128, 282
DriI GACNNNNNGTC 1 cut(s) 16
Eam1104I CTCTTC 2 cut(s) 92, 101
Eam1105I GACNNNNNGTC 1 cut(s) 16
EarI CTCTTC 2 cut(s) 92, 101
Eco31I GGTCTC 2 cut(s) 339, 368
Eco47I GGWCC 1 cut(s) 10
Eco57I CTGAAG 2 cut(s) 164, 187
EcoO109I RGGNCCY 1 cut(s) 10
EcoRI GAATTC 1 cut(s) 162
EcoRII CCWGG 2 cut(s) 113, 208
FaeI CATG 1 cut(s) 373
FaqI GGGAC 1 cut(s) 131
FatI CATG 1 cut(s) 369
FauNDI CATATG 1 cut(s) 417
Fnu4HI GCNGC 1 cut(s) 184
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 1 cut(s) 184
FspBI CTAG 3 cut(s) 219, 318, 350
GluI GCNGC 1 cut(s) 184
Hin1II CATG 1 cut(s) 373
HphI GGTGA 1 cut(s) 319
Hpy188I TCNGA 1 cut(s) 265
Hpy188III TCNNGA 1 cut(s) 318
HpyAV CCTTC 1 cut(s) 139
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4V TGCA 3 cut(s) 50, 108, 415
HpyF3I CTNAG 1 cut(s) 444
Hsp92II CATG 1 cut(s) 373
Kzo9I GATC 2 cut(s) 128, 282
Lsp1109I GCAGC 1 cut(s) 195
MaeI CTAG 3 cut(s) 219, 318, 350
MalI GATC 2 cut(s) 130, 284
MboI GATC 2 cut(s) 128, 282
MboII GAAGA 4 cut(s) 79, 88, 149, 193
MluCI AATT 2 cut(s) 162, 214
MmeI TCCRAC 1 cut(s) 410
MnlI CCTC 4 cut(s) 23, 101, 104, 325
MspA1I CMGCKG 1 cut(s) 426
MspR9I CCNGG 2 cut(s) 115, 210
MvaI CCWGG 2 cut(s) 115, 210
NdeI CATATG 1 cut(s) 417
NdeII GATC 2 cut(s) 128, 282
NlaIII CATG 1 cut(s) 373
NlaIV GGNNCC 1 cut(s) 405
PkrI GCNGC 1 cut(s) 185
PpuMI RGGWCCY 1 cut(s) 10
PsiI TTATAA 1 cut(s) 366
Psp5II RGGWCCY 1 cut(s) 10
Psp6I CCWGG 2 cut(s) 113, 208
PspGI CCWGG 2 cut(s) 113, 208
PspN4I GGNNCC 1 cut(s) 405
PspPI GGNCC 1 cut(s) 10
PspPPI RGGWCCY 1 cut(s) 10
PvuII CAGCTG 1 cut(s) 426
RsaI GTAC 1 cut(s) 422
RsaNI GTAC 1 cut(s) 421
SatI GCNGC 1 cut(s) 184
Sau3AI GATC 2 cut(s) 128, 282
Sau96I GGNCC 1 cut(s) 10
ScrFI CCNGG 2 cut(s) 115, 210
SetI ASST 8 cut(s) 15, 125, 188, 317, 341, 351, 409, 428
SinI GGWCC 1 cut(s) 10
SmlI CTYRAG 1 cut(s) 124
SmoI CTYRAG 1 cut(s) 124
Sse9I AATT 2 cut(s) 162, 214
SsiI CCGC 1 cut(s) 226
SspMI CTAG 3 cut(s) 219, 318, 350
StyD4I CCNGG 2 cut(s) 113, 208
TaaI ACNGT 1 cut(s) 274
TaqI TCGA 1 cut(s) 131
TasI AATT 2 cut(s) 162, 214
TatI WGTACW 1 cut(s) 420
TscAI CASTG 2 cut(s) 279, 405
TseI GCWGC 1 cut(s) 183
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 279, 405
VpaK11BI GGWCC 1 cut(s) 10
XapI RAATTY 2 cut(s) 162, 214
XbaI TCTAGA 1 cut(s) 317
XspI CTAG 3 cut(s) 219, 318, 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.