RLG00000019146

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000112
Physical Location & Seq
Forward (+)
47317478 .. 47327086
9609 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019146

Sequence Viewer

Length: 3453 bp
ATGGCTTCTCTTCTTTCCTTCTCTCTTCCCAACTCAAAGATGATGATAAGAGCTTCTTCACCATCAACTTCTGCAACGACGAGTACCGTGCCAGAGAGCCTTACTGACAGATTTGGCAGAAAAGGCATCAAGTTCGTGGAGTATGACAGCACTCCATTTGTTGAGCTCACAGTCAGAAATGGCAGTTCATTAAAGCTTCAAATACCGAATGCTCATGTAACTTCGTATAAGCCAAAGGTTTATTGGAAAGACGATGGCTTTGAGGAGGTTCTTTACACAATTCCAGGTAAAGGAACCGAGCCTAACAAAGCCAAGGGGGGAATTGGTTTGGTCTTAAATGATGTATCACAGGCCGGTTCTAAAGGGACACTTGTATCCACTTCTGAATGGACAGTGAAAGATGTTGATTCGGATTCCATTGATGCTATGCAGGTTGAATTAAGCAGCACCAGTGGAAAACTTGATATAACATATGTTGTCACACTGTACCCGGTAAGCATTGCAACAGCAGTTAAAGTGAAGAACAAAGGCCGAAAGGATGCAACTCTAACAAGTGCTATACTCAGCCATTTCAAATTTAAGAGACGAAGTGGAGCAGCAATCCAAGGTCTCAAGGGCTGCAGTTACACTGTACAACCTCCACTATCTTCTCCTTTCGAGATTTTGTCACCAAACGAAGCATTGAAAGCTGATCCATCACCTGGGTGGTTCTCTTCTGAGCCTGAAGAAAAACCAGGTTCTTGGACTCAACAAGATGTACCTTACACCATTTTGAAAAACAAGCTCAGCAGAGTTTATGCTGCGCCTCCTAAAGAGAGACTGAAGGCCATTTATAACACCCCTCCTTCAAAATATGATACACTTGATCAGGGACGAGAGCTCTTCTTCAGGGTGATAAGGATGGGATTTGATGACATCTACTTATCCAGCCCTGGTTCTTTGTCCGAGAAATATGGGAATGAGTATTTTATATGCACCGGCCCTGCGTCAATAAAAAGAGAGAGGGGTCCAATGGCTGCTAACAATGTTCCAGTGGGTTTGGACAAGGAACTGATTTTTGGTATGGCAGAGGAGGAGATGGAGTACAGGGTTGAGTTGTTTAACAAAGAGAAAACCCCTCATGATTTTTATAATTTGGACAATCATATACATCAGTTTATGGGAAGTAGCGCGAGCATCGGAGCCAAAAAGGTGAAAGCTGAATGTCAACAGTTCAGGGAATATTGTACGGCAGGAAATGGAGAAGGGTACGGACCTCCATGGGGAAGGACACCACAATCAGAGACCACGGTTCAGTGCTATGAACCTGCAACCATGAAATACTTGGGAGATTACCCTGCATTGACACCTGATGAGTTCTGCACTTCCAGGGACACCATAAAAGGGAATGAAGAACAACTGAAGGATGTTGATGGAGACTATTTAGTGTCCAAAGAAAGCGAGTTTGAATTGGGTTTCTTCAGTCCCGGAAATTCTAGCTACAGGTATGTTGGAATTTGGTATTCTCAGACTAGAGTATCCACTAAAACGGTGGTGTGGGTTGCAAACAGGAACAATCCCATCAATGATACCTCTGGTGTGCTCACAATAAACAGATATGGAGAACTAGTCCTTTATGCTTATAACATGGAGAGCACTCCTATTTGGTCTACTAATGTGTTGCGGTCGGTTCAAAATGTTAACACAAGCACTTTATCTGCACAGCTTTTAGATACAGGAAATTTAGTTGTGTTCCAGGCTGATAATAATGAAATCTATGTATGGCAAAGTTTTGATTATCCTACTGATACTTTAATTCCAGGTATGAAAGTTGGGGTGAATTGGAAAACTGGGCTAGAATGGGTTTTGACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACATTTAGGCTCTATCCATATCAGATCGCATCCCCCGAATTTTTTATGTACAAGGGTTTGAGTAAGTATTACCGAACTGATCCAGGGCCAACGCCTGGTTTGGTCACTAGTCAAGAGGAAACTTATTATTCCATGAATGACACCAATGCAATTACAAGATTAACAGTGACTAATTCTGCGCTCGAGCGCCTTTATTGGGATGATGATGGCCTTCAATGGAAGGTAGACCTCTCTGCACCGAAGACCCGGTGTGACGGGTATGGAAAGTGTGGTGCCAACAGCAGATGTAGCCCTGACAACGTTAGTGTGTTTGAGTGTGAGTGTTTATCAGGGTACGTGCCAAAATCTATGAGTGATTGGAATCAGAAAAATGGGTCGGGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAAGTGTGGAGATGGAGACGGGTTTGTGAAGGTGGCAAGAGTCAAATATCCAGATACATCGATAGCAGCATGGTTAAAATCAGGAATGAGTGCCAAAGAGTGTGAGCAGGAGTGCCTAAGAAATTGTTCTTGCAAAGCATATTTGAGCATTGAAAATGAAGGGATTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTTTTATGTACACAGAATTTGGACGAGATCTCTACGTTCGTGTGAATGCAACCATGTTAGGTACTAATCTGCTTGCAGGTCAGCTACCAAATGAACAAAAAGTTGCTGTGAAAAGATTGTCCAAAACTTCCGGACAAGGGATTGAGGAATTCAAAAATGAAGTTGCACTTATAGCAAGACTTCAACACAGGAATCTTGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATGTTAGTCCTAGAATACATGGCTAACAAAAGCTTGGACTCCTTTCTGTTTGATAACAGAAGACGGTCCTTCTTGGATTGGAAAAAGCGCTTTAAAATTATCAACGGGATTGCTCGTGGGATTCTGTATCTTCACCAAGACTCAAGATTGAAGATTATCCATAGAGATCTTAAAACTAGCAATGTTCTACTAGATGATGAGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATATTCGACCGGGACCAACTGCAAGATAAGACGAAACGAATTTACGCAGTATTTGGGAGATTTTCCACGAAATCGGATGTCTTTAGTTTTGGGATCATAATGTTGGAGATTGTAAGTGGGCAAAAAAACAATAGTTCTGATCTGGAGGATCCTTCCGTGAACTTGATAGGACGTGTTTGGGAGCTCTGGAAAGAAGGTAGAGCCTTAGATATTGTTGATTCGACACTGAAGTCATGTCAGCCTCATGAAGTCATGAGATGCATACAAGTTGCGCTCTTGTGTGTACAAAAAGATTCAAAGGACCGACCTGACATGTCAGCCATTGTTATCATGTTGAGTGGTGAAGCATCTCCTCCATTGCCTAAGCAGCCAGCATTTGTTTTCAGAAGAGATTCGGACGCTGATGTTGATCCATTACTTCCAAACCGATCTTATTCAAAAAACGACTTGACAATAACTACATTGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

1151

Amino Acids

128.97

Weight (kDa)

6.09

Isoelectric Point (pI)

36.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 510 - 620 1.2e-35 D-mannose binding lectin
S_locus_glycop PF00954 668 - 746 5.9e-14 S-locus glycoprotein domain
PAN_2 PF08276 769 - 835 3.5e-17 PAN-like domain
Pkinase PF00069 863 - 1100 2.9e-31 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 864 - 1102 1.6e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 834, 1131, 1623
AasI GACNNNNNNGTC 1 cut(s) 3205
Acc36I ACCTGC 3 cut(s) 421, 1315, 2570
AccB1I GGYRCC 1 cut(s) 2162
AccB7I CCANNNNNTGG 2 cut(s) 701, 1985
AccI GTMKAC 2 cut(s) 1649, 2115
AccII CGCG 1 cut(s) 1172
AccIII TCCGGA 1 cut(s) 2633
AciI CCGC 1 cut(s) 1663
AclI AACGTT 1 cut(s) 2190
AclWI GGATC 6 cut(s) 686, 1964, 3077, 3119, 3132, 3380
AcsI RAATTY 9 cut(s) 575, 1471, 1494, 1720, 1928, 2519, 2651, 2952, 3015
AcuI CTGAAG 6 cut(s) 744, 842, 871, 1421, 1444, 3224
AfeI AGCGCT 1 cut(s) 2828
AfiI CCNNNNNNNGG 8 cut(s) 290, 701, 1262, 1383, 1880, 1985, 2086, 2640
AflIII ACRYGT 2 cut(s) 3148, 3288
AhlI ACTAGT 2 cut(s) 1606, 1997
AjiI CACGTC 1 cut(s) 3149
AjuI GAANNNNNNNTTGG 2 cut(s) 1041, 1073
AleI CACNNNNGTG 1 cut(s) 703
Alw21I GWGCWC 5 cut(s) 168, 882, 1584, 1637, 3162
Alw26I GTCTC 6 cut(s) 577, 614, 811, 1277, 1410, 2310
AlwI GGATC 6 cut(s) 686, 1964, 3077, 3119, 3132, 3380
Ama87I CYCGRG 1 cut(s) 2072
Aor13HI TCCGGA 1 cut(s) 2633
Aor51HI AGCGCT 1 cut(s) 2828
AoxI GGCC 6 cut(s) 351, 529, 825, 979, 1976, 2098
ApeKI GCWGC 7 cut(s) 444, 596, 618, 800, 1016, 2366, 3343
ApoI RAATTY 9 cut(s) 575, 1471, 1494, 1720, 1928, 2519, 2651, 2952, 3015
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
Asp700I GAANNNNTTC 2 cut(s) 2425, 3367
AspLEI GCGC 6 cut(s) 805, 1172, 2071, 2079, 2829, 3250
AspS9I GGNCC 7 cut(s) 980, 1007, 1253, 1976, 2805, 2989, 3277
AsuC2I CCSGG 4 cut(s) 491, 1467, 2137, 2987
AsuHPI GGTGA 8 cut(s) 51, 660, 690, 904, 1204, 1828, 2864, 3329
AvaI CYCGRG 1 cut(s) 2072
AvaII GGWCC 5 cut(s) 1007, 1253, 2805, 2989, 3277
BaeI ACNNNNGTAYC 4 cut(s) 357, 390, 1500, 1533
BamHI GGATCC 1 cut(s) 3124
BanI GGYRCC 1 cut(s) 2162
BanII GRGCYC 3 cut(s) 168, 882, 3162
BauI CACGAG 1 cut(s) 2853
BbsI GAAGAC 2 cut(s) 2138, 2806
Bbv12I GWGCWC 5 cut(s) 168, 882, 1584, 1637, 3162
BbvI GCAGC 7 cut(s) 456, 605, 608, 787, 1003, 2378, 3355
BceAI ACGGC 1 cut(s) 1245
BcgI CGANNNNNNTGC 4 cut(s) 70, 104, 115, 149
BciVI GTATCC 2 cut(s) 385, 1528
BclI TGATCA 1 cut(s) 865
BcnI CCSGG 4 cut(s) 491, 1467, 2137, 2987
BcoDI GTCTC 6 cut(s) 577, 614, 811, 1277, 1410, 2310
BcuI ACTAGT 2 cut(s) 1606, 1997
BfaI CTAG 9 cut(s) 1476, 1512, 1607, 1835, 1998, 2750, 2916, 2930, 2973
BfmI CTRYAG 2 cut(s) 619, 1480
BfoI RGCGCY 2 cut(s) 2080, 2830
BfuAI ACCTGC 3 cut(s) 421, 1315, 2570
BfuI GTATCC 2 cut(s) 385, 1528
BglII AGATCT 2 cut(s) 2530, 2905
BisI GCNGC 7 cut(s) 445, 597, 619, 801, 1017, 2367, 3344
BlpI GCTNAGC 1 cut(s) 785
BlsI GCNGC 7 cut(s) 446, 598, 620, 802, 1018, 2368, 3345
Bme18I GGWCC 5 cut(s) 1007, 1253, 2805, 2989, 3277
BmeT110I CYCGRG 1 cut(s) 2072
BmgBI CACGTC 1 cut(s) 3149
BmgT120I GGNCC 7 cut(s) 980, 1007, 1253, 1976, 2805, 2989, 3277
BmiI GGNNCC 6 cut(s) 295, 1008, 1183, 2164, 2990, 3126
BmrI ACTGGG 2 cut(s) 1839, 1890
BmsI GCATC 7 cut(s) 135, 412, 529, 1185, 1928, 3224, 3332
BmuI ACTGGG 2 cut(s) 1839, 1890
BpiI GAAGAC 2 cut(s) 2138, 2806
BpmI CTGGAG 1 cut(s) 3140
Bpu10I CCTNAGC 1 cut(s) 3339
Bpu1102I GCTNAGC 1 cut(s) 785
BpuEI CTTGAG 2 cut(s) 596, 2866
BpuMI CCSGG 4 cut(s) 491, 1467, 2137, 2987
Bsa29I ATCGAT 1 cut(s) 2360
BsaAI YACGTR 1 cut(s) 2227
BsaBI GATNNNNATC 2 cut(s) 2250, 3384
BsaI GGTCTC 2 cut(s) 614, 1277
BsaJI CCNNGG 9 cut(s) 312, 604, 701, 931, 1259, 1287, 1368, 1873, 1973
BsaWI WCCGGW 1 cut(s) 2633
BsaXI ACNNNNNCTCC 4 cut(s) 1067, 1097, 1593, 1623
Bsc4I CCNNNNNNNGG 8 cut(s) 290, 701, 1262, 1383, 1880, 1985, 2086, 2640
Bse118I RCCGGY 2 cut(s) 353, 977
Bse1I ACTGG 4 cut(s) 450, 1031, 1834, 1885
Bse3DI GCAATG 3 cut(s) 498, 2926, 3332
Bse8I GATNNNNATC 2 cut(s) 2250, 3384
BseAI TCCGGA 1 cut(s) 2633
BseCI ATCGAT 1 cut(s) 2360
BseDI CCNNGG 9 cut(s) 312, 604, 701, 931, 1259, 1287, 1368, 1873, 1973
BseGI GGATG 8 cut(s) 544, 906, 1411, 1919, 2095, 2279, 2745, 3058
BseJI GATNNNNATC 2 cut(s) 2250, 3384
BseLI CCNNNNNNNGG 8 cut(s) 290, 701, 1262, 1383, 1880, 1985, 2086, 2640
BseMI GCAATG 3 cut(s) 498, 2926, 3332
BseMII CTCAG 4 cut(s) 577, 708, 799, 1520
BseNI ACTGG 4 cut(s) 450, 1031, 1834, 1885
BseRI GAGGAG 4 cut(s) 278, 1085, 1088, 3318
BseXI GCAGC 7 cut(s) 456, 605, 608, 787, 1003, 2378, 3355
BsgI GTGCAG 3 cut(s) 1345, 1683, 2109
Bsh1236I CGCG 1 cut(s) 1172
Bsh1285I CGRYCG 2 cut(s) 1667, 2986
BshFI GGCC 6 cut(s) 353, 531, 827, 981, 1978, 2100
BshNI GGYRCC 1 cut(s) 2162
BshVI ATCGAT 1 cut(s) 2360
BsiEI CGRYCG 2 cut(s) 1667, 2986
BsiHKAI GWGCWC 5 cut(s) 168, 882, 1584, 1637, 3162
BsiHKCI CYCGRG 1 cut(s) 2072
BsiSI CCGG 7 cut(s) 354, 491, 978, 1467, 2137, 2634, 2986
BslFI GGGAC 6 cut(s) 379, 885, 1385, 1449, 1898, 3002
BslI CCNNNNNNNGG 8 cut(s) 290, 701, 1262, 1383, 1880, 1985, 2086, 2640
BsmAI GTCTC 6 cut(s) 577, 614, 811, 1277, 1410, 2310
BsmBI CGTCTC 2 cut(s) 577, 2310
BsmFI GGGAC 6 cut(s) 379, 885, 1385, 1449, 1898, 3002
BsmI GAATGC 2 cut(s) 214, 2554
BsnI GGCC 6 cut(s) 353, 531, 827, 981, 1978, 2100
Bso31I GGTCTC 2 cut(s) 614, 1277
BsoBI CYCGRG 1 cut(s) 2072
Bsp1286I GDGCHC 5 cut(s) 168, 882, 1584, 1637, 3162
Bsp13I TCCGGA 1 cut(s) 2633
Bsp1407I TGTACA 4 cut(s) 631, 1938, 2511, 3259
Bsp1720I GCTNAGC 1 cut(s) 785
Bsp19I CCATGG 1 cut(s) 1259
BspACI CCGC 1 cut(s) 1663
BspANI GGCC 6 cut(s) 353, 531, 827, 981, 1978, 2100
BspCNI CTCAG 4 cut(s) 576, 709, 798, 1519
BspDI ATCGAT 1 cut(s) 2360
BspEI TCCGGA 1 cut(s) 2633
BspFNI CGCG 1 cut(s) 1172
BspHI TCATGA 3 cut(s) 1120, 3220, 3228
BspLI GGNNCC 6 cut(s) 295, 1008, 1183, 2164, 2990, 3126
BspMAI CTGCAG 1 cut(s) 623
BspMI ACCTGC 3 cut(s) 421, 1315, 2570
BspPI GGATC 6 cut(s) 686, 1964, 3077, 3119, 3132, 3380
BspQI GCTCTTC 1 cut(s) 887
BspT107I GGYRCC 1 cut(s) 2162
BspTNI GGTCTC 2 cut(s) 614, 1277
BsrDI GCAATG 3 cut(s) 498, 2926, 3332
BsrFI RCCGGY 2 cut(s) 353, 977
BsrGI TGTACA 4 cut(s) 631, 1938, 2511, 3259
BsrI ACTGG 4 cut(s) 450, 1031, 1834, 1885
BssAI RCCGGY 2 cut(s) 353, 977
BssECI CCNNGG 9 cut(s) 312, 604, 701, 931, 1259, 1287, 1368, 1873, 1973
BssSI CACGAG 1 cut(s) 2853
BssT1I CCWWGG 3 cut(s) 312, 604, 1259
Bst2BI CACGAG 1 cut(s) 2853
Bst6I CTCTTC 5 cut(s) 15, 30, 718, 887, 3358
BstAUI TGTACA 4 cut(s) 631, 1938, 2511, 3259
BstBAI YACGTR 1 cut(s) 2227
BstC8I GCNNGC 3 cut(s) 1174, 2577, 3348
BstDEI CTNAG 7 cut(s) 563, 717, 785, 1506, 2417, 3181, 3339
BstDSI CCRYGG 2 cut(s) 1259, 1287
BstF5I GGATG 8 cut(s) 544, 906, 1411, 1919, 2095, 2279, 2745, 3058
BstFNI CGCG 1 cut(s) 1172
BstH2I RGCGCY 2 cut(s) 2080, 2830
BstHHI GCGC 6 cut(s) 805, 1172, 2071, 2079, 2829, 3250
BstMAI GTCTC 6 cut(s) 577, 614, 811, 1277, 1410, 2310
BstMCI CGRYCG 2 cut(s) 1667, 2986
BstMWI GCNNNNNNNGC 5 cut(s) 123, 686, 2178, 2675, 3343
BstNSI RCATGY 1 cut(s) 3292
BstSFI CTRYAG 2 cut(s) 619, 1480
BstUI CGCG 1 cut(s) 1172
BstV1I GCAGC 7 cut(s) 456, 605, 608, 787, 1003, 2378, 3355
BstV2I GAAGAC 2 cut(s) 2138, 2806
BstX2I RGATCY 3 cut(s) 2530, 2905, 3124
BstXI CCANNNNNNTGG 1 cut(s) 741
BstYI RGATCY 3 cut(s) 2530, 2905, 3124
Bsu15I ATCGAT 1 cut(s) 2360
BsuI GTATCC 2 cut(s) 385, 1528
BsuRI GGCC 6 cut(s) 353, 531, 827, 981, 1978, 2100
BsuTUI ATCGAT 1 cut(s) 2360
BtgI CCRYGG 2 cut(s) 1259, 1287
BtrI CACGTC 1 cut(s) 3149
BtsCI GGATG 8 cut(s) 544, 906, 1411, 1919, 2095, 2279, 2745, 3058
BtsIMutI CAGTG 8 cut(s) 399, 457, 482, 627, 1038, 1301, 2061, 3200
BveI ACCTGC 3 cut(s) 421, 1315, 2570
Cac8I GCNNGC 3 cut(s) 1174, 2577, 3348
CciI TCATGA 3 cut(s) 1120, 3220, 3228
CfoI GCGC 6 cut(s) 805, 1172, 2071, 2079, 2829, 3250
Cfr10I RCCGGY 2 cut(s) 353, 977
Cfr13I GGNCC 7 cut(s) 980, 1007, 1253, 1976, 2805, 2989, 3277
ClaI ATCGAT 1 cut(s) 2360
CseI GACGC 2 cut(s) 975, 3383
CsiI ACCWGGT 1 cut(s) 733
DdeI CTNAG 7 cut(s) 563, 717, 785, 1506, 2417, 3181, 3339
DraI TTTAAA 1 cut(s) 2833
DrdI GACNNNNNNGTC 1 cut(s) 3205
DseDI GACNNNNNNGTC 1 cut(s) 3205
Eam1104I CTCTTC 5 cut(s) 15, 30, 718, 887, 3358
EarI CTCTTC 5 cut(s) 15, 30, 718, 887, 3358
Ecl136II GAGCTC 3 cut(s) 166, 880, 3160
Eco130I CCWWGG 3 cut(s) 312, 604, 1259
Eco24I GRGCYC 3 cut(s) 168, 882, 3162
Eco31I GGTCTC 2 cut(s) 614, 1277
Eco47I GGWCC 5 cut(s) 1007, 1253, 2805, 2989, 3277
Eco47III AGCGCT 1 cut(s) 2828
Eco53kI GAGCTC 3 cut(s) 166, 880, 3160
Eco57I CTGAAG 6 cut(s) 744, 842, 871, 1421, 1444, 3224
Eco88I CYCGRG 1 cut(s) 2072
EcoICRI GAGCTC 3 cut(s) 166, 880, 3160
EcoRI GAATTC 1 cut(s) 2651
EcoT14I CCWWGG 3 cut(s) 312, 604, 1259
EcoT22I ATGCAT 1 cut(s) 3239
EcoT38I GRGCYC 3 cut(s) 168, 882, 3162
ErhI CCWWGG 3 cut(s) 312, 604, 1259
Esp3I CGTCTC 2 cut(s) 577, 2310
FalI AAGNNNNNCTT 6 cut(s) 40, 72, 354, 386, 2655, 2687
FaqI GGGAC 6 cut(s) 379, 885, 1385, 1449, 1898, 3002
FauNDI CATATG 1 cut(s) 472
FbaI TGATCA 1 cut(s) 865
FblI GTMKAC 2 cut(s) 1649, 2115
Fnu4HI GCNGC 7 cut(s) 445, 597, 619, 801, 1017, 2367, 3344
FokI GGATG 8 cut(s) 551, 913, 1418, 1906, 2102, 2286, 2752, 3065
FriOI GRGCYC 3 cut(s) 168, 882, 3162
Fsp4HI GCNGC 7 cut(s) 445, 597, 619, 801, 1017, 2367, 3344
FspBI CTAG 9 cut(s) 1476, 1512, 1607, 1835, 1998, 2750, 2916, 2930, 2973
GlaI GCGC 6 cut(s) 804, 1171, 2070, 2078, 2828, 3249
GluI GCNGC 7 cut(s) 445, 597, 619, 801, 1017, 2367, 3344
GsuI CTGGAG 1 cut(s) 3140
HaeII RGCGCY 2 cut(s) 2080, 2830
HaeIII GGCC 6 cut(s) 353, 531, 827, 981, 1978, 2100
HapII CCGG 7 cut(s) 354, 491, 978, 1467, 2137, 2634, 2986
HgaI GACGC 2 cut(s) 975, 3383
HhaI GCGC 6 cut(s) 805, 1172, 2071, 2079, 2829, 3250
Hin6I GCGC 6 cut(s) 803, 1170, 2069, 2077, 2827, 3248
HinP1I GCGC 6 cut(s) 803, 1170, 2069, 2077, 2827, 3248
HincII GTYRAC 2 cut(s) 1208, 1681
HindII GTYRAC 2 cut(s) 1208, 1681
HindIII AAGCTT 2 cut(s) 194, 2770
HpaI GTTAAC 1 cut(s) 1681
HpaII CCGG 7 cut(s) 354, 491, 978, 1467, 2137, 2634, 2986
HphI GGTGA 8 cut(s) 51, 660, 690, 904, 1204, 1828, 2864, 3329
Hpy166II GTNNAC 7 cut(s) 1208, 1650, 1681, 2116, 2514, 3136, 3260
Hpy8I GTNNAC 7 cut(s) 1208, 1650, 1681, 2116, 2514, 3136, 3260
Hpy99I CGWCG 1 cut(s) 82
HpyCH4IV ACGT 4 cut(s) 2190, 2226, 2538, 3148
HpyF10VI GCNNNNNNNGC 5 cut(s) 123, 686, 2178, 2675, 3343
HpyF3I CTNAG 7 cut(s) 563, 717, 785, 1506, 2417, 3181, 3339
HpySE526I ACGT 4 cut(s) 2190, 2226, 2538, 3148
HspAI GCGC 6 cut(s) 803, 1170, 2069, 2077, 2827, 3248
Kpn2I TCCGGA 1 cut(s) 2633
Ksp22I TGATCA 1 cut(s) 865
KspAI GTTAAC 1 cut(s) 1681
LguI GCTCTTC 1 cut(s) 887
LmnI GCTCC 3 cut(s) 593, 1181, 3157
Lsp1109I GCAGC 7 cut(s) 456, 605, 608, 787, 1003, 2378, 3355
LweI GCATC 7 cut(s) 135, 412, 529, 1185, 1928, 3224, 3332
MabI ACCWGGT 1 cut(s) 733
MaeI CTAG 9 cut(s) 1476, 1512, 1607, 1835, 1998, 2750, 2916, 2930, 2973
MaeII ACGT 4 cut(s) 2190, 2226, 2538, 3148
MaeIII GTNAC 8 cut(s) 217, 478, 623, 666, 1860, 1993, 2056, 2141
MflI RGATCY 3 cut(s) 2530, 2905, 3124
MhlI GDGCHC 5 cut(s) 168, 882, 1584, 1637, 3162
MlyI GAGTC 4 cut(s) 739, 2349, 2771, 2873
MmeI TCCRAC 2 cut(s) 1471, 3060
Mph1103I ATGCAT 1 cut(s) 3239
MroI TCCGGA 1 cut(s) 2633
MroXI GAANNNNTTC 2 cut(s) 2425, 3367
MslI CAYNNNNRTG 2 cut(s) 703, 2507
MspI CCGG 7 cut(s) 354, 491, 978, 1467, 2137, 2634, 2986
Mva1269I GAATGC 2 cut(s) 214, 2554
MvnI CGCG 1 cut(s) 1172
MwoI GCNNNNNNNGC 5 cut(s) 123, 686, 2178, 2675, 3343
NciI CCSGG 4 cut(s) 491, 1467, 2137, 2987
NcoI CCATGG 1 cut(s) 1259
NdeI CATATG 1 cut(s) 472
NlaIV GGNNCC 6 cut(s) 295, 1008, 1183, 2164, 2990, 3126
NmuCI GTSAC 6 cut(s) 478, 666, 1860, 1993, 2056, 2141
NsiI ATGCAT 1 cut(s) 3239
NspI RCATGY 1 cut(s) 3292
OliI CACNNNNGTG 1 cut(s) 703
PaeR7I CTCGAG 1 cut(s) 2072
PagI TCATGA 3 cut(s) 1120, 3220, 3228
PciI ACATGT 1 cut(s) 3288
PciSI GCTCTTC 1 cut(s) 887
PcsI WCGNNNNNNNCGW 1 cut(s) 1923
PctI GAATGC 2 cut(s) 214, 2554
PdmI GAANNNNTTC 2 cut(s) 2425, 3367
PfeI GAWTC 8 cut(s) 407, 413, 2251, 2695, 2860, 3194, 3269, 3368
PflMI CCANNNNNTGG 2 cut(s) 701, 1985
PfoI TCCNGGA 1 cut(s) 1465
PkrI GCNGC 7 cut(s) 446, 598, 620, 802, 1018, 2368, 3345
PleI GAGTC 4 cut(s) 739, 2348, 2771, 2873
PpsI GAGTC 4 cut(s) 739, 2348, 2771, 2873
Ppu21I YACGTR 1 cut(s) 2227
PscI ACATGT 1 cut(s) 3288
PsiI TTATAA 3 cut(s) 834, 1131, 1623
Psp124BI GAGCTC 3 cut(s) 168, 882, 3162
Psp1406I AACGTT 1 cut(s) 2190
PspN4I GGNNCC 6 cut(s) 295, 1008, 1183, 2164, 2990, 3126
PspPI GGNCC 7 cut(s) 980, 1007, 1253, 1976, 2805, 2989, 3277
PspXI VCTCGAGB 1 cut(s) 2072
PstI CTGCAG 1 cut(s) 623
PsuI RGATCY 3 cut(s) 2530, 2905, 3124
RseI CAYNNNNRTG 2 cut(s) 703, 2507
SacI GAGCTC 3 cut(s) 168, 882, 3162
SapI GCTCTTC 1 cut(s) 887
SatI GCNGC 7 cut(s) 445, 597, 619, 801, 1017, 2367, 3344
Sau96I GGNCC 7 cut(s) 980, 1007, 1253, 1976, 2805, 2989, 3277
SchI GAGTC 4 cut(s) 739, 2349, 2771, 2873
SduI GDGCHC 5 cut(s) 168, 882, 1584, 1637, 3162
SexAI ACCWGGT 1 cut(s) 733
SfaNI GCATC 7 cut(s) 135, 412, 529, 1185, 1928, 3224, 3332
SfcI CTRYAG 2 cut(s) 619, 1480
Sfr274I CTCGAG 1 cut(s) 2072
SinI GGWCC 5 cut(s) 1007, 1253, 2805, 2989, 3277
SlaI CTCGAG 1 cut(s) 2072
SmiMI CAYNNNNRTG 2 cut(s) 703, 2507
SmlI CTYRAG 3 cut(s) 611, 2072, 2881
SmoI CTYRAG 3 cut(s) 611, 2072, 2881
SpeI ACTAGT 2 cut(s) 1606, 1997
SsiI CCGC 1 cut(s) 1663
SspI AATATT 2 cut(s) 1223, 2979
SspMI CTAG 9 cut(s) 1476, 1512, 1607, 1835, 1998, 2750, 2916, 2930, 2973
SstI GAGCTC 3 cut(s) 168, 882, 3162
StyI CCWWGG 3 cut(s) 312, 604, 1259
TaiI ACGT 4 cut(s) 2193, 2229, 2541, 3151
TaqI TCGA 7 cut(s) 657, 2073, 2287, 2360, 2982, 3197, 3448
TaqII GACCGA 1 cut(s) 3294
TatI WGTACW 5 cut(s) 631, 1083, 1938, 2511, 3259
TfiI GAWTC 8 cut(s) 407, 413, 2251, 2695, 2860, 3194, 3269, 3368
TscAI CASTG 8 cut(s) 399, 457, 489, 634, 1038, 1301, 2061, 3207
TseFI GTSAC 6 cut(s) 478, 666, 1860, 1993, 2056, 2141
TseI GCWGC 7 cut(s) 444, 596, 618, 800, 1016, 2366, 3343
Tsp45I GTSAC 6 cut(s) 478, 666, 1860, 1993, 2056, 2141
TspGWI ACGGA 2 cut(s) 1266, 3121
TspRI CASTG 8 cut(s) 399, 457, 489, 634, 1038, 1301, 2061, 3207
Van91I CCANNNNNTGG 2 cut(s) 701, 1985
VpaK11BI GGWCC 5 cut(s) 1007, 1253, 2805, 2989, 3277
XapI RAATTY 9 cut(s) 575, 1471, 1494, 1720, 1928, 2519, 2651, 2952, 3015
XceI RCATGY 1 cut(s) 3292
XcmI CCANNNNNNNNNTGG 4 cut(s) 240, 702, 1321, 1528
XhoI CTCGAG 1 cut(s) 2072
XmiI GTMKAC 2 cut(s) 1649, 2115
XmnI GAANNNNTTC 2 cut(s) 2425, 3367
XspI CTAG 9 cut(s) 1476, 1512, 1607, 1835, 1998, 2750, 2916, 2930, 2973
Zsp2I ATGCAT 1 cut(s) 3239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.