Prupe.4G032000_v2.0.a1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000112
Physical Location & Seq
Forward (+)
1486730 .. 1490440
3711 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G032000.2

Sequence Viewer

Length: 2421 bp
ATGCTTTTCAAAGCTTTGCTTGTGTTCTTCCTCCTCCTCTTCCCATTTTGCACATCCATTGACACCATTGCACTGAACCAGCGTGTGAAAGATGGTGACTTTATAGTGTCCAAAGAAAACAACTTTGAATTAGGCTTCTTCAGCCCCGGCAATTCGAGCTCTCGCTATGTTGGAATTTGGTATGCTAATAAGTCTGAAAAGGCAGTGGTTTGGGTTGCAAACAGAAACAACCCCATCAATGATACCTCTGGCGTCCTCACCATCAACAGGTATGGAAATTTGGTTATTTATGCGCACGATATTGACAGCTTTTCTATTTGGTCCGCAAATGTGTCTCTTCAAACCACAAGCACTTCTGTAGCTCAGCTTTTAGATACAGGAAATTTAGTGCTTTTCCCGGATAGTATTAGTAAAAGTGAAAGCATTATATGGCAGAGTTTTGATTATCCTACAGATACTCTACTTCCCGGAATGAAAGTTGGGTTGAATTGGAAAACTGGGTTGGAATGGGTCTTAACATCTTGGAAGTCCCAAAATGACCCTGGAACTGGTAACTATAGCTTAAGGCTCTATTCAAACCAGACTACTACGCCCCAAATTTTTCTGTACAAGGGTTTGACCAAATATTGGAGAAGTGATCCAGGGCCATGGCCCAGTTTTGTGAGTAATAATGAAGAAATGTGTTATTTACTTTTAACTGATAACACCTCTGTAATTAGATCCTTGTTGACGGATCATGGGATACTACAACAACTGAGATGGAATGATGCGGTTAATCAATGGGAGGAACTCTTTGCTGCCCCAAAATATCGGTGTGACCGATATGGACATTGTGGTGCCAACAGCAAATGTAGCCCTGACAATATTAATATCTTTGAATGTGAGTGCCTACCGAGGTATGAACCAAAATCTTTGAATGATTGGAACCGGAGAGATGGGTCAGAGGGGTGTGTGAGTAAGCGAATTGGGGTGCCAAAATGTGGGAGTGGGGATGGGTTTGTGAAGGTGGCACGGGTGAAAGATCCAGACACATCGAAAGCAACACAATTGTTGACAAGTACTAGTGCCAATGAGTGTGAGCGGGTGTGCTTAAGCAATTGTTCCTGCACGGCATATATGAGCATAGAACGGGAAGGTCGCATTGATTGCTTGGCATGGTACGGTGAGTTGTGGGACATTTTATTACACACGGAGCTAGGGCGAGATCTATATGTTCGTGTGGACAAAATGGAATTAGCTGATAATACCAGAAAATCGAAAGGTTTTCTCAAAAGGAGGGGTTTGCTGGCTATTGCAATAGTGGCTGTGCTTCTCGCATTCGTACTAATCATTGTGTTTGTGTATTGGTGGCTGAAGAGAAAGAGGACGATAAAAGACTTTGTGGAGGCGGATGAACTTGAGGAAACTAGGAGACACCCAGAGCTGCAATTTTTCCATCTGAGCGCAATAATAGTAGCCACAGACCACTTCTCTCCTGTCAATAAACTTGGCCAAGGTGGTTTTGGCACTGTTTATAAGGGTCTGTTAGCAAATGATCAGAAGATTGCTGTAAAAAGATTGTCCAAAACTTCAGGACAAGGAATTGAAGAATTCAAAAATGAAGTTGCTCTGATAGCTAGACTTCAACACAGAAATCTTGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATGTTAGTCCTGGAATACTTGCCGAACAAAAGCTTGGACTCCTTTCTTTTTGATCACACAAGACGTTCCTTGTTGGATTGGAAAAAGCGCTCTGAAATTATCAATGGGGTTGCTCGTGGGATTCTGTATCTTCACCAGGATTCAAGACTTAGGATTATTCATAGGGATCTAAAAACCAGCAATGTTCTTCTAGATGCTGAGATGAACCCAAAAATATCAGATTTTGGCATGGCAAGAATATTCCATGGGGACCAACTGCAGGATAAAACCGACAGAGTTGTTGGAACATATGGATATATGTCACCTGAGTATGCAGTGTTTGGAAGATATTCAACCAAATCAGATGTCTTTAGTTTTGGGATCATATTATTGGAGATTGTGAGTGGCAAAAAAAACAATGGCTCTTATCAAGAGGAACATTCCATGAACTTGATAGGACATGTTTGGCAGCTGTGGAGAGAAGATAGAGCCTTAGAAATTGTGGATTCATCACTAGAGTCATATCATTCTGACGAAGTCATGAGATGCATTCAAGTCGGGCTCTTGTGCGTGCAAGAAGACGCAGAGGACCGACCAACCATGTCAGCCGTTGTATTAATGTTGAGTGGTGAAGCATCTCTACCATCTCCTCAGCAGCCTGCATTTGTCTTCAGAAAAAGTTCCTGCGGTGGTGGTTATCTATCAAGTCCACAAGGATTATATTCTGTTAACGACTTGACAATGACTAAACTTGTAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

807

Amino Acids

91.31

Weight (kDa)

7.18

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1515
Acc16I TGCGCA 1 cut(s) 294
AccB1I GGYRCC 2 cut(s) 836, 970
AccB7I CCANNNNNTGG 2 cut(s) 627, 980
AccBSI CCGCTC 1 cut(s) 1081
AciI CCGC 5 cut(s) 324, 770, 1081, 1388, 2343
AclWI GGATC 6 cut(s) 632, 714, 741, 1016, 1851, 2045
AcoI YGGCCR 1 cut(s) 1489
AcsI RAATTY 5 cut(s) 174, 277, 382, 597, 1589
AcuI CTGAAG 4 cut(s) 124, 1373, 1554, 2311
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 4 cut(s) 608, 1060, 1160, 1323
AfeI AGCGCT 1 cut(s) 1766
AfiI CCNNNNNNNGG 5 cut(s) 267, 548, 627, 980, 1936
AflII CTTAAG 2 cut(s) 562, 1090
AflIII ACRYGT 1 cut(s) 2116
AhlI ACTAGT 1 cut(s) 1061
AjnI CCWGG 4 cut(s) 541, 640, 1686, 1812
AjuI GAANNNNNNNTTGG 4 cut(s) 485, 517, 1694, 1726
Alw21I GWGCWC 1 cut(s) 161
Alw26I GTCTC 2 cut(s) 339, 1405
AlwI GGATC 6 cut(s) 632, 714, 741, 1016, 1851, 2045
Aor51HI AGCGCT 1 cut(s) 1766
AoxI GGCC 3 cut(s) 644, 650, 1489
ApeKI GCWGC 4 cut(s) 797, 1423, 2125, 2311
ApoI RAATTY 5 cut(s) 174, 277, 382, 597, 1589
ArsI GACNNNNNNTTYG 2 cut(s) 2007, 2039
AseI ATTAAT 2 cut(s) 867, 2273
Asp700I GAANNNNTTC 2 cut(s) 2005, 2335
AspLEI GCGC 3 cut(s) 295, 1445, 1767
AspS9I GGNCC 5 cut(s) 321, 644, 651, 1927, 2245
AsuC2I CCSGG 3 cut(s) 147, 398, 468
AsuHPI GGTGA 7 cut(s) 107, 250, 1027, 1175, 1802, 1971, 2297
AvaII GGWCC 3 cut(s) 321, 1927, 2245
BalI TGGCCA 1 cut(s) 1491
BanI GGYRCC 2 cut(s) 836, 970
BanII GRGCYC 2 cut(s) 161, 2220
BarI GAAGNNNNNNTAC 2 cut(s) 2280, 2312
BauI CACGAG 1 cut(s) 1791
BbsI GAAGAC 2 cut(s) 2241, 2317
Bbv12I GWGCWC 1 cut(s) 161
BbvCI CCTCAGC 1 cut(s) 2307
BbvI GCAGC 4 cut(s) 784, 1410, 2137, 2323
BccI CCATC 8 cut(s) 86, 242, 269, 753, 929, 986, 1443, 2308
BceAI ACGGC 2 cut(s) 1125, 2249
BcgI CGANNNNNNTGC 2 cut(s) 2194, 2228
BciT130I CCWGG 4 cut(s) 543, 642, 1688, 1814
BciVI GTATCC 1 cut(s) 735
BclI TGATCA 2 cut(s) 1534, 1729
BcnI CCSGG 3 cut(s) 147, 398, 468
BcoDI GTCTC 2 cut(s) 339, 1405
BcuI ACTAGT 1 cut(s) 1061
BfaI CTAG 6 cut(s) 1062, 1196, 1407, 1617, 1868, 2171
BfmI CTRYAG 4 cut(s) 357, 450, 556, 1934
BfoI RGCGCY 1 cut(s) 1768
BfrI CTTAAG 2 cut(s) 562, 1090
BfuI GTATCC 1 cut(s) 735
BglII AGATCT 1 cut(s) 1204
BisI GCNGC 4 cut(s) 798, 1424, 2126, 2312
BlpI GCTNAGC 1 cut(s) 363
BlsI GCNGC 4 cut(s) 799, 1425, 2127, 2313
BmcAI AGTACT 1 cut(s) 1060
Bme1390I CCNGG 7 cut(s) 147, 398, 468, 543, 642, 1688, 1814
Bme18I GGWCC 3 cut(s) 321, 1927, 2245
BmgT120I GGNCC 5 cut(s) 321, 644, 651, 1927, 2245
BmiI GGNNCC 4 cut(s) 838, 926, 972, 1928
BmrFI CCNGG 7 cut(s) 147, 398, 468, 543, 642, 1688, 1814
BmrI ACTGGG 2 cut(s) 507, 648
BmsI GCATC 4 cut(s) 757, 1861, 2192, 2300
BmuI ACTGGG 2 cut(s) 507, 648
BpiI GAAGAC 2 cut(s) 2241, 2317
Bpu10I CCTNAGC 1 cut(s) 2307
Bpu1102I GCTNAGC 1 cut(s) 363
BpuEI CTTGAG 1 cut(s) 1418
BpuMI CCSGG 3 cut(s) 147, 398, 468
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 7 cut(s) 145, 541, 641, 647, 893, 1492, 1921
BsaWI WCCGGW 1 cut(s) 927
Bsc4I CCNNNNNNNGG 5 cut(s) 267, 548, 627, 980, 1936
Bse1I ACTGG 3 cut(s) 502, 553, 654
Bse3DI GCAATG 2 cut(s) 66, 1864
BseBI CCWGG 4 cut(s) 543, 642, 1688, 1814
BseDI CCNNGG 7 cut(s) 145, 541, 641, 647, 893, 1492, 1921
BseGI GGATG 4 cut(s) 53, 997, 1396, 1683
BseLI CCNNNNNNNGG 5 cut(s) 267, 548, 627, 980, 1936
BseMI GCAATG 2 cut(s) 66, 1864
BseMII CTCAG 6 cut(s) 377, 746, 1430, 1866, 1974, 2321
BseNI ACTGG 3 cut(s) 502, 553, 654
BseRI GAGGAG 3 cut(s) 23, 26, 2295
BseXI GCAGC 4 cut(s) 784, 1410, 2137, 2323
BsgI GTGCAG 1 cut(s) 1090
BshFI GGCC 3 cut(s) 646, 652, 1491
BshNI GGYRCC 2 cut(s) 836, 970
BsiHKAI GWGCWC 1 cut(s) 161
BsiSI CCGG 4 cut(s) 147, 398, 468, 928
BslFI GGGAC 3 cut(s) 514, 1187, 1940
BslI CCNNNNNNNGG 5 cut(s) 267, 548, 627, 980, 1936
BsmAI GTCTC 2 cut(s) 339, 1405
BsmFI GGGAC 3 cut(s) 514, 1187, 1940
BsmI GAATGC 2 cut(s) 1316, 2205
BsnI GGCC 3 cut(s) 646, 652, 1491
Bsp1286I GDGCHC 2 cut(s) 161, 2220
Bsp1407I TGTACA 1 cut(s) 606
Bsp143I GATC 9 cut(s) 637, 719, 733, 1021, 1204, 1534, 1729, 1843, 2037
Bsp1720I GCTNAGC 1 cut(s) 363
Bsp19I CCATGG 2 cut(s) 647, 1921
BspACI CCGC 5 cut(s) 324, 770, 1081, 1388, 2343
BspANI GGCC 3 cut(s) 646, 652, 1491
BspCNI CTCAG 6 cut(s) 376, 747, 1431, 1867, 1975, 2320
BspHI TCATGA 1 cut(s) 2196
BspLI GGNNCC 4 cut(s) 838, 926, 972, 1928
BspMAI CTGCAG 1 cut(s) 1938
BspPI GGATC 6 cut(s) 632, 714, 741, 1016, 1851, 2045
BspT107I GGYRCC 2 cut(s) 836, 970
BspTI CTTAAG 2 cut(s) 562, 1090
BsrBI CCGCTC 1 cut(s) 1081
BsrDI GCAATG 2 cut(s) 66, 1864
BsrGI TGTACA 1 cut(s) 606
BsrI ACTGG 3 cut(s) 502, 553, 654
BssECI CCNNGG 7 cut(s) 145, 541, 641, 647, 893, 1492, 1921
BssMI GATC 9 cut(s) 637, 719, 733, 1021, 1204, 1534, 1729, 1843, 2037
BssNI GRCGYC 1 cut(s) 252
BssSI CACGAG 1 cut(s) 1791
BssT1I CCWWGG 3 cut(s) 647, 1492, 1921
Bst2BI CACGAG 1 cut(s) 1791
Bst2UI CCWGG 4 cut(s) 543, 642, 1688, 1814
Bst4CI ACNGT 2 cut(s) 1163, 1510
Bst6I CTCTTC 3 cut(s) 44, 342, 1349
BstACI GRCGYC 1 cut(s) 252
BstAFI CTTAAG 2 cut(s) 562, 1090
BstAPI GCANNNNNTGC 1 cut(s) 1146
BstAUI TGTACA 1 cut(s) 606
BstC8I GCNNGC 3 cut(s) 1287, 2228, 2316
BstDEI CTNAG 8 cut(s) 363, 755, 1439, 1826, 1875, 1983, 2149, 2307
BstDSI CCRYGG 2 cut(s) 647, 1921
BstF5I GGATG 4 cut(s) 53, 997, 1396, 1683
BstH2I RGCGCY 1 cut(s) 1768
BstHHI GCGC 3 cut(s) 295, 1445, 1767
BstKTI GATC 9 cut(s) 640, 722, 736, 1024, 1207, 1537, 1732, 1846, 2040
BstMAI GTCTC 2 cut(s) 339, 1405
BstMBI GATC 9 cut(s) 637, 719, 733, 1021, 1204, 1534, 1729, 1843, 2037
BstMWI GCNNNNNNNGC 6 cut(s) 141, 156, 852, 1146, 1301, 1613
BstNI CCWGG 4 cut(s) 543, 642, 1688, 1814
BstNSI RCATGY 1 cut(s) 2120
BstSCI CCNGG 7 cut(s) 145, 396, 466, 541, 640, 1686, 1812
BstSFI CTRYAG 4 cut(s) 357, 450, 556, 1934
BstV1I GCAGC 4 cut(s) 784, 1410, 2137, 2323
BstV2I GAAGAC 2 cut(s) 2241, 2317
BstX2I RGATCY 4 cut(s) 719, 1021, 1204, 1843
BstXI CCANNNNNNTGG 1 cut(s) 648
BstYI RGATCY 4 cut(s) 719, 1021, 1204, 1843
BsuI GTATCC 1 cut(s) 735
BsuRI GGCC 3 cut(s) 646, 652, 1491
BtgI CCRYGG 2 cut(s) 647, 1921
BtsCI GGATG 4 cut(s) 53, 997, 1396, 1683
BtsI GCAGTG 2 cut(s) 210, 1998
BtsIMutI CAGTG 4 cut(s) 71, 210, 1506, 1998
Cac8I GCNNGC 3 cut(s) 1287, 2228, 2316
CciI TCATGA 1 cut(s) 2196
CfoI GCGC 3 cut(s) 295, 1445, 1767
Cfr13I GGNCC 5 cut(s) 321, 644, 651, 1927, 2245
CseI GACGC 2 cut(s) 241, 2246
Csp6I GTAC 4 cut(s) 607, 1059, 1159, 1322
CviAII CATG 9 cut(s) 648, 737, 1155, 1906, 1922, 2101, 2117, 2197, 2257
CviQI GTAC 4 cut(s) 607, 1059, 1159, 1322
DdeI CTNAG 8 cut(s) 363, 755, 1439, 1826, 1875, 1983, 2149, 2307
DpnI GATC 9 cut(s) 639, 721, 735, 1023, 1206, 1536, 1731, 1845, 2039
DpnII GATC 9 cut(s) 637, 719, 733, 1021, 1204, 1534, 1729, 1843, 2037
EaeI YGGCCR 1 cut(s) 1489
Eam1104I CTCTTC 3 cut(s) 44, 342, 1349
EarI CTCTTC 3 cut(s) 44, 342, 1349
EciI GGCGGA 1 cut(s) 1403
Ecl136II GAGCTC 1 cut(s) 159
Eco130I CCWWGG 3 cut(s) 647, 1492, 1921
Eco24I GRGCYC 2 cut(s) 161, 2220
Eco47I GGWCC 3 cut(s) 321, 1927, 2245
Eco47III AGCGCT 1 cut(s) 1766
Eco53kI GAGCTC 1 cut(s) 159
Eco57I CTGAAG 4 cut(s) 124, 1373, 1554, 2311
EcoICRI GAGCTC 1 cut(s) 159
EcoRI GAATTC 1 cut(s) 1589
EcoRII CCWGG 4 cut(s) 541, 640, 1686, 1812
EcoT14I CCWWGG 3 cut(s) 647, 1492, 1921
EcoT22I ATGCAT 1 cut(s) 2207
EcoT38I GRGCYC 2 cut(s) 161, 2220
ErhI CCWWGG 3 cut(s) 647, 1492, 1921
FaeI CATG 9 cut(s) 651, 740, 1158, 1909, 1925, 2104, 2120, 2200, 2260
FalI AAGNNNNNCTT 1 cut(s) 35
FaqI GGGAC 3 cut(s) 514, 1187, 1940
FatI CATG 9 cut(s) 647, 736, 1154, 1905, 1921, 2100, 2116, 2196, 2256
FauI CCCGC 1 cut(s) 1074
FauNDI CATATG 1 cut(s) 1966
FbaI TGATCA 2 cut(s) 1534, 1729
Fnu4HI GCNGC 4 cut(s) 798, 1424, 2126, 2312
FokI GGATG 4 cut(s) 40, 1004, 1403, 1690
FriOI GRGCYC 2 cut(s) 161, 2220
Fsp4HI GCNGC 4 cut(s) 798, 1424, 2126, 2312
FspAI RTGCGCAY 1 cut(s) 294
FspBI CTAG 6 cut(s) 1062, 1196, 1407, 1617, 1868, 2171
FspI TGCGCA 1 cut(s) 294
GlaI GCGC 3 cut(s) 294, 1444, 1766
GluI GCNGC 4 cut(s) 798, 1424, 2126, 2312
HaeII RGCGCY 1 cut(s) 1768
HaeIII GGCC 3 cut(s) 646, 652, 1491
HapII CCGG 4 cut(s) 147, 398, 468, 928
HgaI GACGC 2 cut(s) 241, 2246
HhaI GCGC 3 cut(s) 295, 1445, 1767
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 9 cut(s) 651, 740, 1158, 1909, 1925, 2104, 2120, 2200, 2260
Hin6I GCGC 3 cut(s) 293, 1443, 1765
HinP1I GCGC 3 cut(s) 293, 1443, 1765
HincII GTYRAC 3 cut(s) 729, 1053, 2386
HindII GTYRAC 3 cut(s) 729, 1053, 2386
HindIII AAGCTT 2 cut(s) 12, 1708
HinfI GANTC 5 cut(s) 1715, 1798, 1817, 2162, 2174
HpaI GTTAAC 1 cut(s) 2386
HpaII CCGG 4 cut(s) 147, 398, 468, 928
HphI GGTGA 7 cut(s) 107, 250, 1027, 1175, 1802, 1971, 2297
Hpy166II GTNNAC 5 cut(s) 729, 1053, 1222, 2366, 2386
Hpy188III TCNNGA 6 cut(s) 1025, 1572, 1821, 1868, 2087, 2197
Hpy8I GTNNAC 5 cut(s) 729, 1053, 1222, 2366, 2386
HpyAV CCTTC 2 cut(s) 997, 1127
HpyCH4III ACNGT 2 cut(s) 1163, 1510
HpyCH4IV ACGT 1 cut(s) 1741
HpyF10VI GCNNNNNNNGC 6 cut(s) 141, 156, 852, 1146, 1301, 1613
HpyF3I CTNAG 8 cut(s) 363, 755, 1439, 1826, 1875, 1983, 2149, 2307
HpySE526I ACGT 1 cut(s) 1741
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 9 cut(s) 651, 740, 1158, 1909, 1925, 2104, 2120, 2200, 2260
HspAI GCGC 3 cut(s) 293, 1443, 1765
Ksp22I TGATCA 2 cut(s) 1534, 1729
KspAI GTTAAC 1 cut(s) 2386
Kzo9I GATC 9 cut(s) 637, 719, 733, 1021, 1204, 1534, 1729, 1843, 2037
LmnI GCTCC 1 cut(s) 1192
Lsp1109I GCAGC 4 cut(s) 784, 1410, 2137, 2323
LweI GCATC 4 cut(s) 757, 1861, 2192, 2300
MaeI CTAG 6 cut(s) 1062, 1196, 1407, 1617, 1868, 2171
MaeII ACGT 1 cut(s) 1741
MaeIII GTNAC 4 cut(s) 95, 551, 815, 1977
MalI GATC 9 cut(s) 639, 721, 735, 1023, 1206, 1536, 1731, 1845, 2039
MbiI CCGCTC 1 cut(s) 1081
MboI GATC 9 cut(s) 637, 719, 733, 1021, 1204, 1534, 1729, 1843, 2037
MfeI CAATTG 2 cut(s) 1046, 1096
MflI RGATCY 4 cut(s) 719, 1021, 1204, 1843
MhlI GDGCHC 2 cut(s) 161, 2220
MlsI TGGCCA 1 cut(s) 1491
MluNI TGGCCA 1 cut(s) 1491
MlyI GAGTC 2 cut(s) 1709, 2183
MmeI TCCRAC 4 cut(s) 151, 483, 1731, 1939
Mox20I TGGCCA 1 cut(s) 1491
Mph1103I ATGCAT 1 cut(s) 2207
MroXI GAANNNNTTC 2 cut(s) 2005, 2335
MscI TGGCCA 1 cut(s) 1491
MseI TTAA 8 cut(s) 515, 563, 695, 774, 867, 1091, 2273, 2385
MslI CAYNNNNRTG 1 cut(s) 834
Msp20I TGGCCA 1 cut(s) 1491
MspA1I CMGCKG 1 cut(s) 2128
MspCI CTTAAG 2 cut(s) 562, 1090
MspI CCGG 4 cut(s) 147, 398, 468, 928
MspR9I CCNGG 7 cut(s) 147, 398, 468, 543, 642, 1688, 1814
MunI CAATTG 2 cut(s) 1046, 1096
Mva1269I GAATGC 2 cut(s) 1316, 2205
MvaI CCWGG 4 cut(s) 543, 642, 1688, 1814
MwoI GCNNNNNNNGC 6 cut(s) 141, 156, 852, 1146, 1301, 1613
NciI CCSGG 3 cut(s) 147, 398, 468
NcoI CCATGG 2 cut(s) 647, 1921
NdeI CATATG 1 cut(s) 1966
NdeII GATC 9 cut(s) 637, 719, 733, 1021, 1204, 1534, 1729, 1843, 2037
NlaIII CATG 9 cut(s) 651, 740, 1158, 1909, 1925, 2104, 2120, 2200, 2260
NlaIV GGNNCC 4 cut(s) 838, 926, 972, 1928
NmuCI GTSAC 3 cut(s) 95, 815, 1977
NsbI TGCGCA 1 cut(s) 294
NsiI ATGCAT 1 cut(s) 2207
NspI RCATGY 1 cut(s) 2120
PagI TCATGA 1 cut(s) 2196
PciI ACATGT 1 cut(s) 2116
PcsI WCGNNNNNNNCGW 1 cut(s) 817
PctI GAATGC 2 cut(s) 1316, 2205
PdmI GAANNNNTTC 2 cut(s) 2005, 2335
PfeI GAWTC 3 cut(s) 1798, 1817, 2162
PflFI GACNNNGTC 1 cut(s) 2192
PflMI CCANNNNNTGG 2 cut(s) 627, 980
PfoI TCCNGGA 3 cut(s) 396, 466, 1686
PkrI GCNGC 4 cut(s) 799, 1425, 2127, 2313
PleI GAGTC 2 cut(s) 1709, 2182
PpsI GAGTC 2 cut(s) 1709, 2182
PscI ACATGT 1 cut(s) 2116
PshBI ATTAAT 2 cut(s) 867, 2273
PsiI TTATAA 1 cut(s) 1515
Psp124BI GAGCTC 1 cut(s) 161
Psp6I CCWGG 4 cut(s) 541, 640, 1686, 1812
PspGI CCWGG 4 cut(s) 541, 640, 1686, 1812
PspN4I GGNNCC 4 cut(s) 838, 926, 972, 1928
PspPI GGNCC 5 cut(s) 321, 644, 651, 1927, 2245
PstI CTGCAG 1 cut(s) 1938
PsuI RGATCY 4 cut(s) 719, 1021, 1204, 1843
PsyI GACNNNGTC 1 cut(s) 2192
PvuII CAGCTG 1 cut(s) 2128
RsaI GTAC 4 cut(s) 608, 1060, 1160, 1323
RsaNI GTAC 4 cut(s) 607, 1059, 1159, 1322
RseI CAYNNNNRTG 1 cut(s) 834
SacI GAGCTC 1 cut(s) 161
SaqAI TTAA 8 cut(s) 515, 563, 695, 774, 867, 1091, 2273, 2385
SatI GCNGC 4 cut(s) 798, 1424, 2126, 2312
Sau3AI GATC 9 cut(s) 637, 719, 733, 1021, 1204, 1534, 1729, 1843, 2037
Sau96I GGNCC 5 cut(s) 321, 644, 651, 1927, 2245
ScaI AGTACT 1 cut(s) 1060
SchI GAGTC 2 cut(s) 1709, 2183
ScrFI CCNGG 7 cut(s) 147, 398, 468, 543, 642, 1688, 1814
SduI GDGCHC 2 cut(s) 161, 2220
SfaNI GCATC 4 cut(s) 757, 1861, 2192, 2300
SfcI CTRYAG 4 cut(s) 357, 450, 556, 1934
SinI GGWCC 3 cut(s) 321, 1927, 2245
SmiMI CAYNNNNRTG 1 cut(s) 834
SmlI CTYRAG 3 cut(s) 562, 1090, 1397
SmoI CTYRAG 3 cut(s) 562, 1090, 1397
SpeI ACTAGT 1 cut(s) 1061
SsiI CCGC 5 cut(s) 324, 770, 1081, 1388, 2343
SspI AATATT 3 cut(s) 626, 865, 1917
SspMI CTAG 6 cut(s) 1062, 1196, 1407, 1617, 1868, 2171
SstI GAGCTC 1 cut(s) 161
StyD4I CCNGG 7 cut(s) 145, 396, 466, 541, 640, 1686, 1812
StyI CCWWGG 3 cut(s) 647, 1492, 1921
TaaI ACNGT 2 cut(s) 1163, 1510
TaiI ACGT 1 cut(s) 1744
TaqI TCGA 4 cut(s) 155, 1034, 1256, 2416
TaqII GACCGA 2 cut(s) 834, 2262
TatI WGTACW 2 cut(s) 606, 1058
TfiI GAWTC 3 cut(s) 1798, 1817, 2162
Tru1I TTAA 8 cut(s) 515, 563, 695, 774, 867, 1091, 2273, 2385
Tru9I TTAA 8 cut(s) 515, 563, 695, 774, 867, 1091, 2273, 2385
TscAI CASTG 4 cut(s) 78, 210, 1513, 1998
TseFI GTSAC 3 cut(s) 95, 815, 1977
TseI GCWGC 4 cut(s) 797, 1423, 2125, 2311
Tsp45I GTSAC 3 cut(s) 95, 815, 1977
TspDTI ATGAA 9 cut(s) 488, 687, 915, 1407, 1614, 1826, 1895, 2117, 2154
TspGWI ACGGA 2 cut(s) 746, 1205
TspRI CASTG 4 cut(s) 78, 210, 1513, 1998
Tth111I GACNNNGTC 1 cut(s) 2192
Van91I CCANNNNNTGG 2 cut(s) 627, 980
Vha464I CTTAAG 2 cut(s) 562, 1090
VpaK11BI GGWCC 3 cut(s) 321, 1927, 2245
VspI ATTAAT 2 cut(s) 867, 2273
XapI RAATTY 5 cut(s) 174, 277, 382, 597, 1589
XbaI TCTAGA 1 cut(s) 1867
XceI RCATGY 1 cut(s) 2120
XcmI CCANNNNNNNNNTGG 2 cut(s) 539, 1499
XmnI GAANNNNTTC 2 cut(s) 2005, 2335
XspI CTAG 6 cut(s) 1062, 1196, 1407, 1617, 1868, 2171
ZrmI AGTACT 1 cut(s) 1060
Zsp2I ATGCAT 1 cut(s) 2207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.