RLG00000031068

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000112
Physical Location & Seq
Forward (+)
1724309 .. 1727514
3206 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031068

Sequence Viewer

Length: 2214 bp
ATGTTGGTCAAAGCTCTGCATCTCTTCCTCCTCTTCCGATTTTGCGATTCCAGGGACACCTTAACATGGAACCAAGAACAACTGAAGGATGATGGTGGAGTTTTAGTGTCCAAACAGAACAAGTTCGAGTTGGGTTTCTTCAGCCCTGGAAATTCTAGCTACCGGTATATTGGAATTCGGTATTCTCAGACTAAAGTACCTGAAAAAACAGTGGTGTGGGTTGCAAACAGAAACAATCCCATCAATGATTCCTCTGGTGTGCTCAAAATAAACAGGTATGGAGAACTGGCCCTTTATGCTTATAACATGAACAGCTCTCCTGTTTGGTCCACTAATGTGTCACGGCCGGTCCAAAATAATAACGCAAGCACTTTTTCTGCACAGCTTTTAGATGCAGGAAATTTAGTTGTGTTCCAGGATGATAATAATGAAATCTATGTATGGCAAAGTTTTGATTATCCTACCGATACTCAAATTCCAGGTATGAAAGTCGGGGTGAATTGGAAAACTGGGCTAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGACTTGGGGACTATACCTTTCGGCTTTATTTAGATCAGACTGCCGCCCCCCAATATTTTATATATAAAGGTTTGAGCAAGTATTGGCGAAGTGATTCAGGGCCAGAGGGTCCTTTTGTCACTAATCAAGAGGAAACTCATAATTTCCTAAATTACAGCAATGCAATTACAAGAACGACGTTGACCGATTCTGGTAGGTTAGATCTCCTTACATGGGATAATGGTGGCCTTCAATGGAAGGAAGACTATTCTGCACCGAAGTCCCAGTGTGACTGGTATGGACAGTGTGGTGCCAACAGCAAATGTAGTCCTGACAATATTAATGTGTTTGAGTGTGAATGTTTACCAGGGTATGAGCCTAGTTCTATAAATGAATGGAATCAGAAAAATGGGTCGGGTGGATGTGTGAGTAAGCGAGTTGGTGTATCAAAGTGCGGGAATGGAGATGGGTTTGTGAATGTGGCAAGAGTTAAATATCCAGACACATCAATAGCATCACGGTTGAGAACAGGTATGAGTGCCAAAGAGTGTGAGCAGGACTGCTCAAGAAACTGTTCTTGCACCGCATACTTGAGCATGGAACATGAAGGGCTTGTGGATTGCTTGACATGGTATGATGACTTGATGGACATTTTAGTGTACACAGAGCGCGGACGAGATTTATACATTCGTGTGAATAAAACAGAGTTAGTTAAACTGCTTAATTTAGTTGTCATGAATCATATGCCTCTTAAAAACATGGACTTAAATTATGATGGTCAGCTACCAAATGAACAGAAAGTTGCTGTGAAAAGATTGTCCAAAACTTCAGGACAAGGGATTGTAGAATTCAAAAATGAAGTTGCACTTATAGCAAGACTTCAACACAGGAACCTTGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATATTAGTCCTAGAATACATGCCTAACAAAAGCTTGGACTCCTTTCTTTTTGATAACACAAGACGGTCCTTTTTGGATTGGGAAAAGCGCTTTGAAATTATCAACGGGATTGCTCGTGGGATTCTATATCTTCACCAAGACTCAAGATTGAGGATTATCCATAGAGATCTAAAAACTAGCAATGTTCTACTAGATGCTGAGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATATTCCACGGGGACCAACTGCAAGATAAGACGAAACGAATTGTCGGAACATATGGCTACATGTCACCGGAGTACGCAGTATTTGGAAGATTTTCCACAAAATCTGATGTCTTTAGTTTTGGGATCATAATGTTGGAGATTTTAAGTGGCCAGAAAAACAATGGTTTTGATCTGGAGGATCCTTCCATGAATTTAATAAGACGTGTTTGGGAGCTCTGGGGAGAAGGCAGAGCCTTAGATATTGTGGATTCGACACTGAAGTCATATCAGCCTAATGAAGTCATGAGATGCATACAAGTTGCACTCTTGTGTGTACAAGAAGATTCGAAGGACCGACCTGCCATGTCAACCATTGTTTTCATGTTGAGTGGTGAAGCATCTCCTCCATTGCCTAACCAGCCGGCATTTGTTTTTAGAAGAAATTCCGGCACTGATGTTTATCCATTACTTTTAAACAGATCTTGTTCTATAAACGATGTGACAGCATCTACAATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

738

Amino Acids

83.74

Weight (kDa)

5.93

Isoelectric Point (pI)

41.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 70 - 180 2.3e-35 D-mannose binding lectin
S_locus_glycop PF00954 224 - 306 3.1e-15 S-locus glycoprotein domain
PAN_2 PF08276 330 - 396 7.6e-17 PAN-like domain
PK_Tyr_Ser-Thr PF07714 442 - 689 3.6e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 442 - 687 7e-39 Protein kinase domain
DUF3403 PF11883 698 - 737 4.2e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AasI GACNNNNNNGTC 1 cut(s) 1966
Acc36I ACCTGC 1 cut(s) 2053
AccB1I GGYRCC 1 cut(s) 845
AccII CGCG 1 cut(s) 1206
AciI CCGC 4 cut(s) 600, 990, 1119, 1206
AclWI GGATC 3 cut(s) 1838, 1880, 1893
AcoI YGGCCR 2 cut(s) 344, 1855
AcsI RAATTY 8 cut(s) 151, 174, 400, 474, 1382, 1683, 1897, 2128
AcuI CTGAAG 4 cut(s) 104, 124, 1347, 1985
AfaI GTAC 4 cut(s) 198, 1196, 1781, 2022
AfeI AGCGCT 1 cut(s) 1559
AfiI CCNNNNNNNGG 3 cut(s) 66, 560, 769
AflIII ACRYGT 2 cut(s) 1767, 1909
AgeI ACCGGT 1 cut(s) 162
AgsI TTSAA 4 cut(s) 788, 1387, 1418, 1565
AjiI CACGTC 1 cut(s) 1910
AjnI CCWGG 6 cut(s) 50, 145, 414, 478, 553, 901
AleI CACNNNNGTG 2 cut(s) 335, 2014
AluBI AGCT 8 cut(s) 14, 159, 315, 385, 1318, 1503, 1921, 2207
AluI AGCT 8 cut(s) 14, 159, 315, 385, 1318, 1503, 1921, 2207
Alw21I GWGCWC 2 cut(s) 264, 1923
AlwI GGATC 3 cut(s) 1838, 1880, 1893
Aor51HI AGCGCT 1 cut(s) 1559
AoxI GGCC 5 cut(s) 288, 344, 656, 781, 1855
ApoI RAATTY 8 cut(s) 151, 174, 400, 474, 1382, 1683, 1897, 2128
AseI ATTAAT 1 cut(s) 876
AsiGI ACCGGT 1 cut(s) 162
Asp700I GAANNNNTTC 5 cut(s) 122, 649, 1108, 1798, 2128
AspLEI GCGC 2 cut(s) 1206, 1560
AspS9I GGNCC 8 cut(s) 289, 327, 349, 656, 665, 1536, 1720, 2038
AsuHPI GGTGA 4 cut(s) 508, 1595, 1764, 2090
AsuII TTCGAA 1 cut(s) 2033
AvaII GGWCC 6 cut(s) 327, 349, 665, 1536, 1720, 2038
BaeI ACNNNNGTAYC 2 cut(s) 180, 213
BalI TGGCCA 1 cut(s) 1857
BamHI GGATCC 1 cut(s) 1885
BanI GGYRCC 1 cut(s) 845
BanII GRGCYC 1 cut(s) 1923
BauI CACGAG 1 cut(s) 1584
BbsI GAAGAC 1 cut(s) 804
Bbv12I GWGCWC 2 cut(s) 264, 1923
BccI CCATC 5 cut(s) 86, 248, 995, 1174, 1304
BceAI ACGGC 1 cut(s) 359
BciT130I CCWGG 6 cut(s) 52, 147, 416, 480, 555, 903
BfaI CTAG 7 cut(s) 156, 515, 915, 1481, 1647, 1661, 1704
BfoI RGCGCY 1 cut(s) 1561
BfuAI ACCTGC 1 cut(s) 2053
BglII AGATCT 3 cut(s) 757, 1636, 2165
BisI GCNGC 1 cut(s) 600
BlsI GCNGC 1 cut(s) 601
Bme1390I CCNGG 6 cut(s) 52, 147, 416, 480, 555, 903
Bme18I GGWCC 6 cut(s) 327, 349, 665, 1536, 1720, 2038
BmgBI CACGTC 1 cut(s) 1910
BmgT120I GGNCC 8 cut(s) 289, 327, 349, 656, 665, 1536, 1720, 2038
BmiI GGNNCC 6 cut(s) 71, 666, 847, 1427, 1721, 1887
BmrFI CCNGG 6 cut(s) 52, 147, 416, 480, 555, 903
BmrI ACTGGG 2 cut(s) 519, 814
BmsI GCATC 7 cut(s) 28, 382, 1058, 1654, 1985, 2093, 2201
BmuI ACTGGG 2 cut(s) 519, 814
BpiI GAAGAC 1 cut(s) 804
BpmI CTGGAG 1 cut(s) 1901
Bpu14I TTCGAA 1 cut(s) 2033
BpuEI CTTGAG 3 cut(s) 1084, 1147, 1597
BsaBI GATNNNNATC 1 cut(s) 2145
BsaJI CCNNGG 5 cut(s) 51, 145, 553, 902, 1714
BsaWI WCCGGW 2 cut(s) 162, 1774
BsaXI ACNNNNNCTCC 2 cut(s) 90, 120
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 560, 769
Bse118I RCCGGY 3 cut(s) 162, 346, 2107
Bse1I ACTGG 4 cut(s) 291, 514, 820, 833
Bse3DI GCAATG 3 cut(s) 721, 1657, 2093
Bse8I GATNNNNATC 1 cut(s) 2145
BseBI CCWGG 6 cut(s) 52, 147, 416, 480, 555, 903
BseDI CCNNGG 5 cut(s) 51, 145, 553, 902, 1714
BseGI GGATG 3 cut(s) 94, 424, 962
BseJI GATNNNNATC 1 cut(s) 2145
BseLI CCNNNNNNNGG 3 cut(s) 66, 560, 769
BseMI GCAATG 3 cut(s) 721, 1657, 2093
BseMII CTCAG 2 cut(s) 200, 1659
BseNI ACTGG 4 cut(s) 291, 514, 820, 833
BseRI GAGGAG 2 cut(s) 20, 2079
BseX3I CGGCCG 1 cut(s) 344
BsgI GTGCAG 2 cut(s) 363, 792
Bsh1236I CGCG 1 cut(s) 1206
Bsh1285I CGRYCG 1 cut(s) 347
BshFI GGCC 5 cut(s) 290, 346, 658, 783, 1857
BshNI GGYRCC 1 cut(s) 845
BshTI ACCGGT 1 cut(s) 162
BsiEI CGRYCG 1 cut(s) 347
BsiHKAI GWGCWC 2 cut(s) 264, 1923
BsiSI CCGG 5 cut(s) 163, 347, 1775, 2108, 2133
BslFI GGGAC 4 cut(s) 68, 578, 802, 1733
BslI CCNNNNNNNGG 3 cut(s) 66, 560, 769
BsmFI GGGAC 4 cut(s) 68, 578, 802, 1733
BsnI GGCC 5 cut(s) 290, 346, 658, 783, 1857
Bsp119I TTCGAA 1 cut(s) 2033
Bsp1286I GDGCHC 2 cut(s) 264, 1923
Bsp1407I TGTACA 2 cut(s) 1194, 2020
Bsp143I GATC 7 cut(s) 589, 757, 1636, 1830, 1876, 1885, 2165
BspACI CCGC 4 cut(s) 600, 990, 1119, 1206
BspANI GGCC 5 cut(s) 290, 346, 658, 783, 1857
BspCNI CTCAG 2 cut(s) 199, 1660
BspFNI CGCG 1 cut(s) 1206
BspHI TCATGA 2 cut(s) 1269, 1989
BspLI GGNNCC 6 cut(s) 71, 666, 847, 1427, 1721, 1887
BspMI ACCTGC 1 cut(s) 2053
BspPI GGATC 3 cut(s) 1838, 1880, 1893
BspT104I TTCGAA 1 cut(s) 2033
BspT107I GGYRCC 1 cut(s) 845
BsrDI GCAATG 3 cut(s) 721, 1657, 2093
BsrFI RCCGGY 3 cut(s) 162, 346, 2107
BsrGI TGTACA 2 cut(s) 1194, 2020
BsrI ACTGG 4 cut(s) 291, 514, 820, 833
BssAI RCCGGY 3 cut(s) 162, 346, 2107
BssECI CCNNGG 5 cut(s) 51, 145, 553, 902, 1714
BssMI GATC 7 cut(s) 589, 757, 1636, 1830, 1876, 1885, 2165
BssSI CACGAG 1 cut(s) 1584
Bst2BI CACGAG 1 cut(s) 1584
Bst2UI CCWGG 6 cut(s) 52, 147, 416, 480, 555, 903
Bst4CI ACNGT 5 cut(s) 211, 840, 1056, 1109, 1536
Bst6I CTCTTC 2 cut(s) 29, 38
BstAUI TGTACA 2 cut(s) 1194, 2020
BstBI TTCGAA 1 cut(s) 2033
BstC8I GCNNGC 2 cut(s) 367, 2109
BstDEI CTNAG 3 cut(s) 186, 1668, 1942
BstDSI CCRYGG 1 cut(s) 1714
BstF5I GGATG 3 cut(s) 94, 424, 962
BstFNI CGCG 1 cut(s) 1206
BstH2I RGCGCY 1 cut(s) 1561
BstHHI GCGC 2 cut(s) 1206, 1560
BstKTI GATC 7 cut(s) 592, 760, 1639, 1833, 1879, 1888, 2168
BstMBI GATC 7 cut(s) 589, 757, 1636, 1830, 1876, 1885, 2165
BstMCI CGRYCG 1 cut(s) 347
BstMWI GCNNNNNNNGC 3 cut(s) 296, 1406, 2104
BstNI CCWGG 6 cut(s) 52, 147, 416, 480, 555, 903
BstNSI RCATGY 2 cut(s) 1492, 1771
BstSCI CCNGG 6 cut(s) 50, 145, 414, 478, 553, 901
BstUI CGCG 1 cut(s) 1206
BstV2I GAAGAC 1 cut(s) 804
BstX2I RGATCY 4 cut(s) 757, 1636, 1885, 2165
BstYI RGATCY 4 cut(s) 757, 1636, 1885, 2165
BstZI CGGCCG 1 cut(s) 344
BsuRI GGCC 5 cut(s) 290, 346, 658, 783, 1857
BtgI CCRYGG 1 cut(s) 1714
BtrI CACGTC 1 cut(s) 1910
BtsCI GGATG 3 cut(s) 94, 424, 962
BtsIMutI CAGTG 5 cut(s) 216, 827, 845, 1961, 2136
BveI ACCTGC 1 cut(s) 2053
Cac8I GCNNGC 2 cut(s) 367, 2109
CciI TCATGA 2 cut(s) 1269, 1989
CfoI GCGC 2 cut(s) 1206, 1560
Cfr10I RCCGGY 3 cut(s) 162, 346, 2107
Cfr13I GGNCC 8 cut(s) 289, 327, 349, 656, 665, 1536, 1720, 2038
Csp6I GTAC 4 cut(s) 197, 1195, 1780, 2021
CspAI ACCGGT 1 cut(s) 162
CviQI GTAC 4 cut(s) 197, 1195, 1780, 2021
DdeI CTNAG 3 cut(s) 186, 1668, 1942
DpnI GATC 7 cut(s) 591, 759, 1638, 1832, 1878, 1887, 2167
DpnII GATC 7 cut(s) 589, 757, 1636, 1830, 1876, 1885, 2165
DraI TTTAAA 1 cut(s) 2160
DrdI GACNNNNNNGTC 1 cut(s) 1966
DseDI GACNNNNNNGTC 1 cut(s) 1966
EaeI YGGCCR 2 cut(s) 344, 1855
EagI CGGCCG 1 cut(s) 344
Eam1104I CTCTTC 2 cut(s) 29, 38
EarI CTCTTC 2 cut(s) 29, 38
Ecl136II GAGCTC 1 cut(s) 1921
EclXI CGGCCG 1 cut(s) 344
Eco24I GRGCYC 1 cut(s) 1923
Eco47I GGWCC 6 cut(s) 327, 349, 665, 1536, 1720, 2038
Eco47III AGCGCT 1 cut(s) 1559
Eco52I CGGCCG 1 cut(s) 344
Eco53kI GAGCTC 1 cut(s) 1921
Eco57I CTGAAG 4 cut(s) 104, 124, 1347, 1985
EcoICRI GAGCTC 1 cut(s) 1921
EcoO109I RGGNCCY 1 cut(s) 665
EcoRI GAATTC 2 cut(s) 174, 1382
EcoRII CCWGG 6 cut(s) 50, 145, 414, 478, 553, 901
EcoT22I ATGCAT 1 cut(s) 2000
EcoT38I GRGCYC 1 cut(s) 1923
FalI AAGNNNNNCTT 2 cut(s) 1386, 1418
FaqI GGGAC 4 cut(s) 68, 578, 802, 1733
FauI CCCGC 1 cut(s) 983
FauNDI CATATG 2 cut(s) 1278, 1759
Fnu4HI GCNGC 1 cut(s) 600
FokI GGATG 3 cut(s) 101, 431, 969
FriOI GRGCYC 1 cut(s) 1923
Fsp4HI GCNGC 1 cut(s) 600
FspBI CTAG 7 cut(s) 156, 515, 915, 1481, 1647, 1661, 1704
GlaI GCGC 2 cut(s) 1205, 1559
GluI GCNGC 1 cut(s) 600
GsuI CTGGAG 1 cut(s) 1901
HaeII RGCGCY 1 cut(s) 1561
HaeIII GGCC 5 cut(s) 290, 346, 658, 783, 1857
HapII CCGG 5 cut(s) 163, 347, 1775, 2108, 2133
HhaI GCGC 2 cut(s) 1206, 1560
Hin6I GCGC 2 cut(s) 1204, 1558
HinP1I GCGC 2 cut(s) 1204, 1558
HincII GTYRAC 2 cut(s) 738, 2055
HindII GTYRAC 2 cut(s) 738, 2055
HindIII AAGCTT 1 cut(s) 1501
HpaII CCGG 5 cut(s) 163, 347, 1775, 2108, 2133
HphI GGTGA 4 cut(s) 508, 1595, 1764, 2090
Hpy166II GTNNAC 7 cut(s) 330, 738, 899, 1195, 1197, 2021, 2055
Hpy188I TCNGA 7 cut(s) 38, 189, 594, 939, 1690, 1754, 1813
Hpy188III TCNNGA 9 cut(s) 683, 866, 1034, 1101, 1270, 1365, 1614, 1880, 1990
Hpy8I GTNNAC 7 cut(s) 330, 738, 899, 1195, 1197, 2021, 2055
Hpy99I CGWCG 1 cut(s) 736
HpyAV CCTTC 7 cut(s) 79, 787, 794, 1136, 1899, 1925, 2029
HpyCH4III ACNGT 5 cut(s) 211, 840, 1056, 1109, 1536
HpyCH4IV ACGT 2 cut(s) 734, 1909
HpyF10VI GCNNNNNNNGC 3 cut(s) 296, 1406, 2104
HpyF3I CTNAG 3 cut(s) 186, 1668, 1942
HpySE526I ACGT 2 cut(s) 734, 1909
HspAI GCGC 2 cut(s) 1204, 1558
KroI GCCGGC 1 cut(s) 2107
KroNI GCCGGC 1 cut(s) 2109
Kzo9I GATC 7 cut(s) 589, 757, 1636, 1830, 1876, 1885, 2165
LmnI GCTCC 1 cut(s) 1918
LweI GCATC 7 cut(s) 28, 382, 1058, 1654, 1985, 2093, 2201
MaeI CTAG 7 cut(s) 156, 515, 915, 1481, 1647, 1661, 1704
MaeII ACGT 2 cut(s) 734, 1909
MaeIII GTNAC 6 cut(s) 339, 540, 673, 824, 1770, 2185
MalI GATC 7 cut(s) 591, 759, 1638, 1832, 1878, 1887, 2167
MboI GATC 7 cut(s) 589, 757, 1636, 1830, 1876, 1885, 2165
MboII GAAGA 9 cut(s) 16, 25, 130, 809, 1475, 1592, 1806, 2039, 2136
MflI RGATCY 4 cut(s) 757, 1636, 1885, 2165
MhlI GDGCHC 2 cut(s) 264, 1923
MlsI TGGCCA 1 cut(s) 1857
MluNI TGGCCA 1 cut(s) 1857
MlyI GAGTC 2 cut(s) 1502, 1604
MmeI TCCRAC 2 cut(s) 1732, 1821
MnlI CCTC 9 cut(s) 38, 41, 262, 655, 679, 1293, 1614, 1876, 2100
Mox20I TGGCCA 1 cut(s) 1857
Mph1103I ATGCAT 1 cut(s) 2000
MroNI GCCGGC 1 cut(s) 2107
MroXI GAANNNNTTC 5 cut(s) 122, 649, 1108, 1798, 2128
MscI TGGCCA 1 cut(s) 1857
MslI CAYNNNNRTG 4 cut(s) 335, 1190, 1226, 2014
Msp20I TGGCCA 1 cut(s) 1857
MspI CCGG 5 cut(s) 163, 347, 1775, 2108, 2133
MspR9I CCNGG 6 cut(s) 52, 147, 416, 480, 555, 903
MvaI CCWGG 6 cut(s) 52, 147, 416, 480, 555, 903
MvnI CGCG 1 cut(s) 1206
MwoI GCNNNNNNNGC 3 cut(s) 296, 1406, 2104
NaeI GCCGGC 1 cut(s) 2109
NdeI CATATG 2 cut(s) 1278, 1759
NdeII GATC 7 cut(s) 589, 757, 1636, 1830, 1876, 1885, 2165
NgoMIV GCCGGC 1 cut(s) 2107
NlaIV GGNNCC 6 cut(s) 71, 666, 847, 1427, 1721, 1887
NmuCI GTSAC 6 cut(s) 339, 540, 673, 824, 1770, 2185
NsiI ATGCAT 1 cut(s) 2000
NspI RCATGY 2 cut(s) 1492, 1771
NspV TTCGAA 1 cut(s) 2033
OliI CACNNNNGTG 2 cut(s) 335, 2014
PagI TCATGA 2 cut(s) 1269, 1989
PciI ACATGT 1 cut(s) 1767
PdiI GCCGGC 1 cut(s) 2109
PdmI GAANNNNTTC 5 cut(s) 122, 649, 1108, 1798, 2128
PfeI GAWTC 9 cut(s) 47, 248, 650, 743, 934, 1273, 1591, 1955, 2030
PfoI TCCNGGA 1 cut(s) 414
PinAI ACCGGT 1 cut(s) 162
PkrI GCNGC 1 cut(s) 601
PleI GAGTC 2 cut(s) 1502, 1604
PpsI GAGTC 2 cut(s) 1502, 1604
PpuMI RGGWCCY 1 cut(s) 665
PscI ACATGT 1 cut(s) 1767
PshBI ATTAAT 1 cut(s) 876
PsiI TTATAA 1 cut(s) 303
Psp124BI GAGCTC 1 cut(s) 1923
Psp5II RGGWCCY 1 cut(s) 665
Psp6I CCWGG 6 cut(s) 50, 145, 414, 478, 553, 901
PspGI CCWGG 6 cut(s) 50, 145, 414, 478, 553, 901
PspN4I GGNNCC 6 cut(s) 71, 666, 847, 1427, 1721, 1887
PspPI GGNCC 8 cut(s) 289, 327, 349, 656, 665, 1536, 1720, 2038
PspPPI RGGWCCY 1 cut(s) 665
PsuI RGATCY 4 cut(s) 757, 1636, 1885, 2165
RsaI GTAC 4 cut(s) 198, 1196, 1781, 2022
RsaNI GTAC 4 cut(s) 197, 1195, 1780, 2021
RseI CAYNNNNRTG 4 cut(s) 335, 1190, 1226, 2014
SacI GAGCTC 1 cut(s) 1923
SatI GCNGC 1 cut(s) 600
Sau3AI GATC 7 cut(s) 589, 757, 1636, 1830, 1876, 1885, 2165
Sau96I GGNCC 8 cut(s) 289, 327, 349, 656, 665, 1536, 1720, 2038
SchI GAGTC 2 cut(s) 1502, 1604
ScrFI CCNGG 6 cut(s) 52, 147, 416, 480, 555, 903
SduI GDGCHC 2 cut(s) 264, 1923
SfaNI GCATC 7 cut(s) 28, 382, 1058, 1654, 1985, 2093, 2201
SfuI TTCGAA 1 cut(s) 2033
SinI GGWCC 6 cut(s) 327, 349, 665, 1536, 1720, 2038
SmiMI CAYNNNNRTG 4 cut(s) 335, 1190, 1226, 2014
SmlI CTYRAG 3 cut(s) 1099, 1126, 1612
SmoI CTYRAG 3 cut(s) 1099, 1126, 1612
SsiI CCGC 4 cut(s) 600, 990, 1119, 1206
SspI AATATT 3 cut(s) 611, 874, 1710
SspMI CTAG 7 cut(s) 156, 515, 915, 1481, 1647, 1661, 1704
SstI GAGCTC 1 cut(s) 1923
StyD4I CCNGG 6 cut(s) 50, 145, 414, 478, 553, 901
TaaI ACNGT 5 cut(s) 211, 840, 1056, 1109, 1536
TaiI ACGT 2 cut(s) 737, 1912
TaqI TCGA 4 cut(s) 126, 1958, 2033, 2209
TaqII GACCGA 2 cut(s) 755, 2055
TatI WGTACW 2 cut(s) 1194, 2020
TauI GCSGC 1 cut(s) 602
TfiI GAWTC 9 cut(s) 47, 248, 650, 743, 934, 1273, 1591, 1955, 2030
TscAI CASTG 5 cut(s) 216, 827, 845, 1968, 2143
TseFI GTSAC 6 cut(s) 339, 540, 673, 824, 1770, 2185
Tsp45I GTSAC 6 cut(s) 339, 540, 673, 824, 1770, 2185
TspRI CASTG 5 cut(s) 216, 827, 845, 1968, 2143
VpaK11BI GGWCC 6 cut(s) 327, 349, 665, 1536, 1720, 2038
VspI ATTAAT 1 cut(s) 876
XapI RAATTY 8 cut(s) 151, 174, 400, 474, 1382, 1683, 1897, 2128
XceI RCATGY 2 cut(s) 1492, 1771
XcmI CCANNNNNNNNNTGG 1 cut(s) 1865
XmnI GAANNNNTTC 5 cut(s) 122, 649, 1108, 1798, 2128
XspI CTAG 7 cut(s) 156, 515, 915, 1481, 1647, 1661, 1704
Zsp2I ATGCAT 1 cut(s) 2000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.