Rh7AG365000

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000112
Physical Location & Seq
Forward (+)
47031725 .. 47032378
654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG365000.1

Sequence Viewer

Length: 654 bp
ATGTGGCCATGGAGCCCATCACCGAGTACAACACCTTATGCATATGACTACACTTTTGTCAATAATCAAGATGTGACATATTTGATTGATTCTTCTAATAAATCCTCTGAAATTTCAAGACTTGTGGTTAACTATTATGGCACACTTAAGCACCTAACATGGAATGATGTTGAGGGTCAATGGAAGGAAAAGTGGGCTGCACCAAAGTTCAGGTGTGACCCATACGGACATTGTGGCGCCTTCAGTGGATGTTGTCTTGACAATGTTCAATCTGAGTGTTCTTGTTTGCCAGGGTATCAACCAAGCTCTTCAGAGAATTGGTCTGAGAAAATTTGGTCAGATGGGTGTGTGAGTAAGCAGGTAGAAGTACCTAACAAGTGTGGAAAAAGTTTCGTGAGGGTGGAACATGTGAAGCTTCTAGATGCATCAATATCAGCTTTGCTGGACACAAATATGGGTGAAAGTAAGTGCAAGAGGGCATGCCAGAATAATTGTTCATGCGTTGCATATTCAAGTCTAGAAATTGATGGAGGTGGTGGTTGCATGGCATGGTATGGGGAATTGGTGGACATTTTAATACAGGCAGAGCAGGGACGAGATTTCTATGTTCGAGCAGATGCAATTGAGTCAGGTACTACTCGAATGAAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

217

Amino Acids

24.23

Weight (kDa)

5.11

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 13 - 102 8e-19 S-locus glycoprotein domain
PAN_2 PF08276 128 - 191 2.1e-16 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 349
AccB1I GGYRCC 1 cut(s) 236
AcoI YGGCCR 1 cut(s) 5
AcsI RAATTY 2 cut(s) 111, 330
AcuI CTGAAG 2 cut(s) 226, 294
AcyI GRCGYC 1 cut(s) 237
AfaI GTAC 3 cut(s) 28, 369, 634
AflII CTTAAG 1 cut(s) 146
AflIII ACRYGT 1 cut(s) 406
AgsI TTSAA 3 cut(s) 117, 269, 513
AjnI CCWGG 1 cut(s) 289
AluBI AGCT 3 cut(s) 306, 415, 437
AluI AGCT 3 cut(s) 306, 415, 437
AoxI GGCC 1 cut(s) 5
ApeKI GCWGC 1 cut(s) 197
ApoI RAATTY 2 cut(s) 111, 330
ArsI GACNNNNNNTTYG 2 cut(s) 38, 70
AspLEI GCGC 1 cut(s) 239
AsuHPI GGTGA 2 cut(s) 12, 470
BalI TGGCCA 1 cut(s) 7
BanI GGYRCC 1 cut(s) 236
BanII GRGCYC 1 cut(s) 17
BbvI GCAGC 1 cut(s) 184
BccI CCATC 3 cut(s) 25, 335, 521
BciT130I CCWGG 1 cut(s) 291
BfaI CTAG 2 cut(s) 419, 518
BfoI RGCGCY 1 cut(s) 240
BfrI CTTAAG 1 cut(s) 146
BfuAI ACCTGC 1 cut(s) 349
BisI GCNGC 1 cut(s) 198
BlsI GCNGC 1 cut(s) 199
Bme1390I CCNGG 1 cut(s) 291
BmiI GGNNCC 2 cut(s) 14, 238
BmrFI CCNGG 1 cut(s) 291
BmsI GCATC 3 cut(s) 412, 434, 607
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 2 cut(s) 8, 290
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 8, 290
BseGI GGATG 1 cut(s) 254
BseMII CTCAG 2 cut(s) 264, 315
BseXI GCAGC 1 cut(s) 184
BsgI GTGCAG 1 cut(s) 183
BshFI GGCC 1 cut(s) 7
BshNI GGYRCC 1 cut(s) 236
BslFI GGGAC 1 cut(s) 606
BsmFI GGGAC 1 cut(s) 606
BsnI GGCC 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 17
Bsp19I CCATGG 1 cut(s) 8
BspANI GGCC 1 cut(s) 7
BspCNI CTCAG 2 cut(s) 265, 316
BspLI GGNNCC 2 cut(s) 14, 238
BspMI ACCTGC 1 cut(s) 349
BspQI GCTCTTC 1 cut(s) 313
BspT107I GGYRCC 1 cut(s) 236
BspTI CTTAAG 1 cut(s) 146
BssECI CCNNGG 2 cut(s) 8, 290
BssNI GRCGYC 1 cut(s) 237
BssT1I CCWWGG 1 cut(s) 8
Bst2UI CCWGG 1 cut(s) 291
Bst6I CTCTTC 1 cut(s) 313
BstACI GRCGYC 1 cut(s) 237
BstAFI CTTAAG 1 cut(s) 146
BstC8I GCNNGC 1 cut(s) 481
BstDEI CTNAG 2 cut(s) 273, 324
BstDSI CCRYGG 1 cut(s) 8
BstF5I GGATG 1 cut(s) 254
BstH2I RGCGCY 1 cut(s) 240
BstHHI GCGC 1 cut(s) 239
BstNI CCWGG 1 cut(s) 291
BstNSI RCATGY 2 cut(s) 410, 483
BstSCI CCNGG 1 cut(s) 289
BstV1I GCAGC 1 cut(s) 184
BsuRI GGCC 1 cut(s) 7
BtgI CCRYGG 1 cut(s) 8
BtsCI GGATG 1 cut(s) 254
BtsIMutI CAGTG 1 cut(s) 250
BveI ACCTGC 1 cut(s) 349
Cac8I GCNNGC 1 cut(s) 481
CfoI GCGC 1 cut(s) 239
Csp6I GTAC 3 cut(s) 27, 368, 633
CspCI CAANNNNNGTGG 2 cut(s) 105, 140
CviAII CATG 7 cut(s) 9, 159, 407, 480, 498, 544, 549
CviJI RGCY 6 cut(s) 7, 15, 197, 306, 415, 437
CviKI_1 RGCY 6 cut(s) 7, 15, 197, 306, 415, 437
CviQI GTAC 3 cut(s) 27, 368, 633
DdeI CTNAG 2 cut(s) 273, 324
DinI GGCGCC 1 cut(s) 238
EaeI YGGCCR 1 cut(s) 5
Eam1104I CTCTTC 1 cut(s) 313
EarI CTCTTC 1 cut(s) 313
Eco130I CCWWGG 1 cut(s) 8
Eco24I GRGCYC 1 cut(s) 17
Eco57I CTGAAG 2 cut(s) 226, 294
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 8
EcoT22I ATGCAT 2 cut(s) 43, 427
EcoT38I GRGCYC 1 cut(s) 17
EgeI GGCGCC 1 cut(s) 238
EheI GGCGCC 1 cut(s) 238
ErhI CCWWGG 1 cut(s) 8
FaeI CATG 7 cut(s) 12, 162, 410, 483, 501, 547, 552
FaqI GGGAC 1 cut(s) 606
FatI CATG 7 cut(s) 8, 158, 406, 479, 497, 543, 548
FauNDI CATATG 1 cut(s) 43
Fnu4HI GCNGC 1 cut(s) 198
FokI GGATG 1 cut(s) 261
FriOI GRGCYC 1 cut(s) 17
Fsp4HI GCNGC 1 cut(s) 198
FspBI CTAG 2 cut(s) 419, 518
GlaI GCGC 1 cut(s) 238
GluI GCNGC 1 cut(s) 198
HaeII RGCGCY 1 cut(s) 240
HaeIII GGCC 1 cut(s) 7
HhaI GCGC 1 cut(s) 239
Hin1I GRCGYC 1 cut(s) 237
Hin1II CATG 7 cut(s) 12, 162, 410, 483, 501, 547, 552
Hin6I GCGC 1 cut(s) 237
HinP1I GCGC 1 cut(s) 237
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 413
HinfI GANTC 2 cut(s) 89, 626
HpaI GTTAAC 1 cut(s) 130
HphI GGTGA 2 cut(s) 12, 470
Hpy166II GTNNAC 2 cut(s) 130, 568
Hpy188I TCNGA 5 cut(s) 109, 274, 313, 325, 340
Hpy188III TCNNGA 6 cut(s) 68, 117, 257, 394, 419, 518
Hpy8I GTNNAC 2 cut(s) 130, 568
HpyAV CCTTC 2 cut(s) 178, 250
HpyCH4V TGCA 7 cut(s) 41, 200, 425, 471, 506, 543, 620
HpyF3I CTNAG 2 cut(s) 273, 324
Hsp92I GRCGYC 1 cut(s) 237
Hsp92II CATG 7 cut(s) 12, 162, 410, 483, 501, 547, 552
HspAI GCGC 1 cut(s) 237
KasI GGCGCC 1 cut(s) 236
KspAI GTTAAC 1 cut(s) 130
LguI GCTCTTC 1 cut(s) 313
LmnI GCTCC 1 cut(s) 12
LpnPI CCDG 9 cut(s) 196, 276, 303, 344, 428, 497, 566, 575, 615
Lsp1109I GCAGC 1 cut(s) 184
LweI GCATC 3 cut(s) 412, 434, 607
MaeI CTAG 2 cut(s) 419, 518
MaeIII GTNAC 2 cut(s) 73, 215
MboII GAAGA 2 cut(s) 84, 300
MfeI CAATTG 1 cut(s) 621
MhlI GDGCHC 1 cut(s) 17
MlsI TGGCCA 1 cut(s) 7
MluCI AATT 8 cut(s) 111, 316, 330, 490, 522, 560, 621, 647
MluNI TGGCCA 1 cut(s) 7
Mly113I GGCGCC 1 cut(s) 237
MlyI GAGTC 1 cut(s) 635
MnlI CCTC 5 cut(s) 115, 166, 390, 468, 524
Mox20I TGGCCA 1 cut(s) 7
Mph1103I ATGCAT 2 cut(s) 43, 427
MscI TGGCCA 1 cut(s) 7
MseI TTAA 3 cut(s) 129, 147, 575
MslI CAYNNNNRTG 1 cut(s) 452
Msp20I TGGCCA 1 cut(s) 7
MspCI CTTAAG 1 cut(s) 146
MspR9I CCNGG 1 cut(s) 291
MunI CAATTG 1 cut(s) 621
MvaI CCWGG 1 cut(s) 291
NarI GGCGCC 1 cut(s) 237
NcoI CCATGG 1 cut(s) 8
NdeI CATATG 1 cut(s) 43
NlaIII CATG 7 cut(s) 12, 162, 410, 483, 501, 547, 552
NlaIV GGNNCC 2 cut(s) 14, 238
NmuCI GTSAC 2 cut(s) 73, 215
NsiI ATGCAT 2 cut(s) 43, 427
NspI RCATGY 2 cut(s) 410, 483
PaeI GCATGC 1 cut(s) 483
PciI ACATGT 1 cut(s) 406
PciSI GCTCTTC 1 cut(s) 313
PfeI GAWTC 1 cut(s) 89
PkrI GCNGC 1 cut(s) 199
PleI GAGTC 1 cut(s) 634
PluTI GGCGCC 1 cut(s) 240
PpsI GAGTC 1 cut(s) 634
PscI ACATGT 1 cut(s) 406
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 2 cut(s) 14, 238
RsaI GTAC 3 cut(s) 28, 369, 634
RsaNI GTAC 3 cut(s) 27, 368, 633
RseI CAYNNNNRTG 1 cut(s) 452
SapI GCTCTTC 1 cut(s) 313
SaqAI TTAA 3 cut(s) 129, 147, 575
SatI GCNGC 1 cut(s) 198
SchI GAGTC 1 cut(s) 635
ScrFI CCNGG 1 cut(s) 291
SduI GDGCHC 1 cut(s) 17
SfaNI GCATC 3 cut(s) 412, 434, 607
SfoI GGCGCC 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 452
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
SphI GCATGC 1 cut(s) 483
Sse9I AATT 8 cut(s) 111, 316, 330, 490, 522, 560, 621, 647
SspDI GGCGCC 1 cut(s) 236
SspMI CTAG 2 cut(s) 419, 518
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 1 cut(s) 8
TaqI TCGA 2 cut(s) 610, 640
TasI AATT 8 cut(s) 111, 316, 330, 490, 522, 560, 621, 647
TatI WGTACW 1 cut(s) 26
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 3 cut(s) 129, 147, 575
Tru9I TTAA 3 cut(s) 129, 147, 575
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 2 cut(s) 73, 215
TseI GCWGC 1 cut(s) 197
Tsp45I GTSAC 2 cut(s) 73, 215
TspDTI ATGAA 1 cut(s) 486
TspGWI ACGGA 1 cut(s) 240
TspRI CASTG 1 cut(s) 250
Vha464I CTTAAG 1 cut(s) 146
XapI RAATTY 2 cut(s) 111, 330
XbaI TCTAGA 2 cut(s) 418, 517
XceI RCATGY 2 cut(s) 410, 483
XspI CTAG 2 cut(s) 419, 518
Zsp2I ATGCAT 2 cut(s) 43, 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.