Rw5G004180

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000112
Physical Location & Seq
Forward (+)
4223907 .. 4225862
1956 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G004180.1

Sequence Viewer

Length: 1251 bp
ATGGATATTTTAGTGTACGCAGAGGTTGGACGAGATCTATATGTTCGTGTGGATCGAATCGAATTAGCTGAGAATTCTAGAAAATCAAAAGGTTTCTTGGAAAGAAGGGGTATGCTGGCTATTCCAATACTCTTTTCTCTGCTAGCACTGGTACTAATCATTATGTTCGCTTGCTGGTGGCGTAAGAAAAAAAGGAATACAACAGAAATTTTGGAGGCGGATGAACTTGAGGAAACTAAGAGACACCCCGAATTGCAATTTTTTGATATGAACACACTAATGGCAGCCACAGATAACTTCTCTCATGTCAATGAACTCGGCCAAGGTGGTTTTGGCGCTGTTTATAAGGGTCAGTTACCAAATGAGCAGAAGGTAGCTGTAAAAAGGTTGTCTAAAACATCAGGACAAGGGACTGAAGAATTCAAAAATGAAGTTGCACTTGTAGCAAGACTTCAACACCGCAATCTCGTAAAACTCTTAGGCTGTTGTATAAAGGGGGAAGAACGAATGTTAGTCCTGGAATACATGCCGAACAAAAGCTTGGACTCATTTCTCTTTGATCACACAAGACGGTCCTTCTTGGATTGGGAAAAGCGCTTTGAAATTATCAACGGGATTGCTCGTGGGATTCTGTATCTTCACCAAGACTCAAGATTGAGGATTATCCATAGAGATCTAAAAACCAGTAATGTTCTTCTAGATGCTGAAATGAACCCAAAAATTTCTGATTTTGGCATGGCAAGAATATTTGACGGGGACCAAATTCAGGATAAAACAAACCGAGTTGTTGGAACATATGGCTATATGTCACCAGAGTATGCAGTATTTGGCAGATTTTCTACTAAATCTGATGTCTTTAGTTTTGGGATCATTTTGTTAGAGGTTGTAACTGGCAAAAGAAACAACGGTTTTTCTCAAGAGGATCCTTCCATGAACTTGATAAGACACGTTTGGGAACTCTGGAGAGAAGATAGAGCCTTAGAACTTGTGGATTCGAATCTGGAGTCATATAATTCTGATGAGGTCATGAGATCCATTCAAGTTGCGCTCTTGTGCGTGCAAGAAGAATCAAAGGACAGGCCAGCCATGTCTGCCATTGTTTTCATGTTGAGTGGTGAAACATCTCCTCCATCACCTAACCAACCTGCATATGTTTTCAGAAGAACCTCCAGCACTGATGCTTATCCATCAGTACCAAAACGATTATATTCTATAAACGACTTGACAATAACTGCAGAGGAAGGTCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

416

Amino Acids

47.75

Weight (kDa)

6.24

Isoelectric Point (pI)

50.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 101 - 364 9.7e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 103 - 368 6.2e-52 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
Acc36I ACCTGC 1 cut(s) 1153
AciI CCGC 2 cut(s) 218, 460
AclWI GGATC 5 cut(s) 60, 875, 917, 930, 1026
AcoI YGGCCR 1 cut(s) 319
AcsI RAATTY 5 cut(s) 73, 207, 419, 720, 762
AcuI CTGAAG 1 cut(s) 435
AfaI GTAC 3 cut(s) 17, 153, 1194
AfeI AGCGCT 1 cut(s) 596
AfiI CCNNNNNNNGG 1 cut(s) 766
AflIII ACRYGT 1 cut(s) 946
AgsI TTSAA 4 cut(s) 424, 455, 602, 1040
AjnI CCWGG 1 cut(s) 516
AjuI GAANNNNNNNTTGG 4 cut(s) 118, 150, 524, 556
AluBI AGCT 3 cut(s) 68, 377, 540
AluI AGCT 3 cut(s) 68, 377, 540
Alw26I GTCTC 1 cut(s) 235
AlwI GGATC 5 cut(s) 60, 875, 917, 930, 1026
Aor51HI AGCGCT 1 cut(s) 596
AoxI GGCC 2 cut(s) 319, 1079
ApeKI GCWGC 1 cut(s) 284
ApoI RAATTY 5 cut(s) 73, 207, 419, 720, 762
ArsI GACNNNNNNTTYG 2 cut(s) 989, 1021
AspLEI GCGC 3 cut(s) 338, 597, 1048
AspS9I GGNCC 2 cut(s) 573, 757
AsuHPI GGTGA 4 cut(s) 632, 801, 1125, 1127
AsuII TTCGAA 1 cut(s) 995
AsuNHI GCTAGC 1 cut(s) 142
AvaII GGWCC 2 cut(s) 573, 757
BamHI GGATCC 1 cut(s) 922
BarI GAAGNNNNNNTAC 2 cut(s) 435, 467
BauI CACGAG 1 cut(s) 621
BbvI GCAGC 1 cut(s) 296
BccI CCATC 2 cut(s) 1138, 1195
BciT130I CCWGG 1 cut(s) 518
BclI TGATCA 1 cut(s) 559
BcoDI GTCTC 1 cut(s) 235
BfaI CTAG 3 cut(s) 78, 143, 698
BfmI CTRYAG 1 cut(s) 1233
BfoI RGCGCY 2 cut(s) 339, 598
BfuAI ACCTGC 1 cut(s) 1153
BglII AGATCT 2 cut(s) 34, 673
BisI GCNGC 1 cut(s) 285
BlsI GCNGC 1 cut(s) 286
Bme1390I CCNGG 1 cut(s) 518
Bme18I GGWCC 2 cut(s) 573, 757
BmgT120I GGNCC 2 cut(s) 573, 757
BmiI GGNNCC 2 cut(s) 758, 924
BmrFI CCNGG 1 cut(s) 518
BmsI GCATC 2 cut(s) 691, 1168
BmtI GCTAGC 1 cut(s) 146
BpmI CTGGAG 3 cut(s) 982, 1022, 1153
Bpu14I TTCGAA 1 cut(s) 995
BpuEI CTTGAG 3 cut(s) 248, 634, 900
BsaBI GATNNNNATC 2 cut(s) 996, 1182
BsaJI CCNNGG 1 cut(s) 322
BsaXI ACNNNNNCTCC 2 cut(s) 1111, 1141
Bsc4I CCNNNNNNNGG 1 cut(s) 766
Bse1I ACTGG 3 cut(s) 153, 684, 895
Bse8I GATNNNNATC 2 cut(s) 996, 1182
BseBI CCWGG 1 cut(s) 518
BseDI CCNNGG 1 cut(s) 322
BseGI GGATG 1 cut(s) 226
BseJI GATNNNNATC 2 cut(s) 996, 1182
BseLI CCNNNNNNNGG 1 cut(s) 766
BseMII CTCAG 1 cut(s) 60
BseNI ACTGG 3 cut(s) 153, 684, 895
BseRI GAGGAG 1 cut(s) 1116
BseXI GCAGC 1 cut(s) 296
BshFI GGCC 2 cut(s) 321, 1081
BslFI GGGAC 2 cut(s) 424, 770
BslI CCNNNNNNNGG 1 cut(s) 766
BsmAI GTCTC 1 cut(s) 235
BsmFI GGGAC 2 cut(s) 424, 770
BsnI GGCC 2 cut(s) 321, 1081
Bsp119I TTCGAA 1 cut(s) 995
Bsp143I GATC 7 cut(s) 34, 52, 559, 673, 867, 922, 1031
BspACI CCGC 2 cut(s) 218, 460
BspANI GGCC 2 cut(s) 321, 1081
BspCNI CTCAG 1 cut(s) 61
BspHI TCATGA 1 cut(s) 1026
BspLI GGNNCC 2 cut(s) 758, 924
BspMAI CTGCAG 1 cut(s) 1237
BspMI ACCTGC 1 cut(s) 1153
BspOI GCTAGC 1 cut(s) 146
BspPI GGATC 5 cut(s) 60, 875, 917, 930, 1026
BspT104I TTCGAA 1 cut(s) 995
BsrI ACTGG 3 cut(s) 153, 684, 895
BssECI CCNNGG 1 cut(s) 322
BssMI GATC 7 cut(s) 34, 52, 559, 673, 867, 922, 1031
BssSI CACGAG 1 cut(s) 621
BssT1I CCWWGG 1 cut(s) 322
Bst2BI CACGAG 1 cut(s) 621
Bst2UI CCWGG 1 cut(s) 518
Bst4CI ACNGT 2 cut(s) 573, 908
BstBI TTCGAA 1 cut(s) 995
BstC8I GCNNGC 5 cut(s) 117, 144, 172, 1058, 1083
BstDEI CTNAG 4 cut(s) 69, 237, 478, 979
BstF5I GGATG 1 cut(s) 226
BstH2I RGCGCY 2 cut(s) 339, 598
BstHHI GCGC 3 cut(s) 338, 597, 1048
BstKTI GATC 7 cut(s) 37, 55, 562, 676, 870, 925, 1034
BstMAI GTCTC 1 cut(s) 235
BstMBI GATC 7 cut(s) 34, 52, 559, 673, 867, 922, 1031
BstMWI GCNNNNNNNGC 2 cut(s) 443, 1091
BstNI CCWGG 1 cut(s) 518
BstNSI RCATGY 1 cut(s) 529
BstSCI CCNGG 1 cut(s) 516
BstSFI CTRYAG 1 cut(s) 1233
BstV1I GCAGC 1 cut(s) 296
BstX2I RGATCY 4 cut(s) 34, 673, 922, 1031
BstYI RGATCY 4 cut(s) 34, 673, 922, 1031
BsuRI GGCC 2 cut(s) 321, 1081
BtsCI GGATG 1 cut(s) 226
BtsIMutI CAGTG 2 cut(s) 146, 1173
BveI ACCTGC 1 cut(s) 1153
Cac8I GCNNGC 5 cut(s) 117, 144, 172, 1058, 1083
CciI TCATGA 1 cut(s) 1026
CfoI GCGC 3 cut(s) 338, 597, 1048
Cfr13I GGNCC 2 cut(s) 573, 757
Csp6I GTAC 3 cut(s) 16, 152, 1193
CviAII CATG 7 cut(s) 305, 526, 736, 931, 1027, 1087, 1105
CviQI GTAC 3 cut(s) 16, 152, 1193
DdeI CTNAG 4 cut(s) 69, 237, 478, 979
DpnI GATC 7 cut(s) 36, 54, 561, 675, 869, 924, 1033
DpnII GATC 7 cut(s) 34, 52, 559, 673, 867, 922, 1031
EaeI YGGCCR 1 cut(s) 319
EciI GGCGGA 1 cut(s) 233
Eco130I CCWWGG 1 cut(s) 322
Eco47I GGWCC 2 cut(s) 573, 757
Eco47III AGCGCT 1 cut(s) 596
Eco57I CTGAAG 1 cut(s) 435
EcoRI GAATTC 2 cut(s) 73, 419
EcoRII CCWGG 1 cut(s) 516
EcoT14I CCWWGG 1 cut(s) 322
ErhI CCWWGG 1 cut(s) 322
FaeI CATG 7 cut(s) 308, 529, 739, 934, 1030, 1090, 1108
FalI AAGNNNNNCTT 2 cut(s) 423, 455
FaqI GGGAC 2 cut(s) 424, 770
FatI CATG 7 cut(s) 304, 525, 735, 930, 1026, 1086, 1104
FauNDI CATATG 2 cut(s) 796, 1150
FbaI TGATCA 1 cut(s) 559
Fnu4HI GCNGC 1 cut(s) 285
FokI GGATG 1 cut(s) 233
Fsp4HI GCNGC 1 cut(s) 285
FspBI CTAG 3 cut(s) 78, 143, 698
GlaI GCGC 3 cut(s) 337, 596, 1047
GluI GCNGC 1 cut(s) 285
GsuI CTGGAG 3 cut(s) 982, 1022, 1153
HaeII RGCGCY 2 cut(s) 339, 598
HaeIII GGCC 2 cut(s) 321, 1081
HhaI GCGC 3 cut(s) 338, 597, 1048
Hin1II CATG 7 cut(s) 308, 529, 739, 934, 1030, 1090, 1108
Hin6I GCGC 3 cut(s) 336, 595, 1046
HinP1I GCGC 3 cut(s) 336, 595, 1046
HindIII AAGCTT 1 cut(s) 538
HinfI GANTC 8 cut(s) 57, 545, 628, 647, 992, 997, 1004, 1067
HphI GGTGA 4 cut(s) 632, 801, 1125, 1127
Hpy166II GTNNAC 1 cut(s) 16
Hpy188I TCNGA 4 cut(s) 727, 850, 1018, 1160
Hpy188III TCNNGA 9 cut(s) 78, 402, 651, 698, 767, 917, 961, 1001, 1027
Hpy8I GTNNAC 1 cut(s) 16
HpyAV CCTTC 5 cut(s) 99, 364, 586, 936, 1235
HpyCH4III ACNGT 2 cut(s) 573, 908
HpyCH4IV ACGT 1 cut(s) 948
HpyCH4V TGCA 6 cut(s) 256, 437, 821, 1060, 1148, 1235
HpyF10VI GCNNNNNNNGC 2 cut(s) 443, 1091
HpyF3I CTNAG 4 cut(s) 69, 237, 478, 979
HpySE526I ACGT 1 cut(s) 948
Hsp92II CATG 7 cut(s) 308, 529, 739, 934, 1030, 1090, 1108
HspAI GCGC 3 cut(s) 336, 595, 1046
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 7 cut(s) 34, 52, 559, 673, 867, 922, 1031
Lsp1109I GCAGC 1 cut(s) 296
LweI GCATC 2 cut(s) 691, 1168
MaeI CTAG 3 cut(s) 78, 143, 698
MaeII ACGT 1 cut(s) 948
MaeIII GTNAC 3 cut(s) 354, 807, 886
MalI GATC 7 cut(s) 36, 54, 561, 675, 869, 924, 1033
MboI GATC 7 cut(s) 34, 52, 559, 673, 867, 922, 1031
MboII GAAGA 7 cut(s) 428, 512, 629, 686, 980, 1076, 1173
MflI RGATCY 4 cut(s) 34, 673, 922, 1031
MlyI GAGTC 3 cut(s) 539, 641, 1013
MmeI TCCRAC 2 cut(s) 7, 769
MslI CAYNNNNRTG 2 cut(s) 278, 309
MspR9I CCNGG 1 cut(s) 518
MvaI CCWGG 1 cut(s) 518
MwoI GCNNNNNNNGC 2 cut(s) 443, 1091
NdeI CATATG 2 cut(s) 796, 1150
NdeII GATC 7 cut(s) 34, 52, 559, 673, 867, 922, 1031
NheI GCTAGC 1 cut(s) 142
NlaIII CATG 7 cut(s) 308, 529, 739, 934, 1030, 1090, 1108
NlaIV GGNNCC 2 cut(s) 758, 924
NmeAIII GCCGAG 1 cut(s) 297
NmuCI GTSAC 1 cut(s) 807
NspI RCATGY 1 cut(s) 529
NspV TTCGAA 1 cut(s) 995
PagI TCATGA 1 cut(s) 1026
PcsI WCGNNNNNNNCGW 1 cut(s) 52
PfeI GAWTC 5 cut(s) 57, 628, 992, 997, 1067
PfoI TCCNGGA 1 cut(s) 516
PkrI GCNGC 1 cut(s) 286
PleI GAGTC 3 cut(s) 539, 641, 1012
PpsI GAGTC 3 cut(s) 539, 641, 1012
PsiI TTATAA 1 cut(s) 345
Psp6I CCWGG 1 cut(s) 516
PspGI CCWGG 1 cut(s) 516
PspN4I GGNNCC 2 cut(s) 758, 924
PspPI GGNCC 2 cut(s) 573, 757
PstI CTGCAG 1 cut(s) 1237
PsuI RGATCY 4 cut(s) 34, 673, 922, 1031
RsaI GTAC 3 cut(s) 17, 153, 1194
RsaNI GTAC 3 cut(s) 16, 152, 1193
RseI CAYNNNNRTG 2 cut(s) 278, 309
SatI GCNGC 1 cut(s) 285
Sau3AI GATC 7 cut(s) 34, 52, 559, 673, 867, 922, 1031
Sau96I GGNCC 2 cut(s) 573, 757
SchI GAGTC 3 cut(s) 539, 641, 1013
ScrFI CCNGG 1 cut(s) 518
SfaNI GCATC 2 cut(s) 691, 1168
SfcI CTRYAG 1 cut(s) 1233
SfuI TTCGAA 1 cut(s) 995
SinI GGWCC 2 cut(s) 573, 757
SmiMI CAYNNNNRTG 2 cut(s) 278, 309
SmlI CTYRAG 3 cut(s) 227, 649, 915
SmoI CTYRAG 3 cut(s) 227, 649, 915
SsiI CCGC 2 cut(s) 218, 460
SspI AATATT 1 cut(s) 747
SspMI CTAG 3 cut(s) 78, 143, 698
StyD4I CCNGG 1 cut(s) 516
StyI CCWWGG 1 cut(s) 322
TaaI ACNGT 2 cut(s) 573, 908
TaiI ACGT 1 cut(s) 951
TaqI TCGA 3 cut(s) 55, 60, 995
TfiI GAWTC 5 cut(s) 57, 628, 992, 997, 1067
TscAI CASTG 2 cut(s) 153, 1180
TseFI GTSAC 1 cut(s) 807
TseI GCWGC 1 cut(s) 284
Tsp45I GTSAC 1 cut(s) 807
TspDTI ATGAA 7 cut(s) 237, 284, 327, 444, 725, 947, 1093
TspRI CASTG 2 cut(s) 153, 1180
VpaK11BI GGWCC 2 cut(s) 573, 757
XapI RAATTY 5 cut(s) 73, 207, 419, 720, 762
XbaI TCTAGA 2 cut(s) 77, 697
XceI RCATGY 1 cut(s) 529
XcmI CCANNNNNNNNNTGG 1 cut(s) 329
XspI CTAG 3 cut(s) 78, 143, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.