MD05G1328000.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000112
Physical Location & Seq
Forward (+)
45351664 .. 45358828
7165 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1328000.v1.1.491

Sequence Viewer

Length: 3162 bp
ATGGTTTTGAGCTATATGAGGGTAGGATTCTTTGGTAGTCCAATGGCTTGGGCCTCTGATCGTCTAATTCTCTATTGCCCACTCAGATGCTGTAATTGTGCCAAGGGTTTGATACATGCATCTGCCAAGAAAATGTTACTGAAAGCTTTGCTTGTTTTTTCCCTCATCTTCCCATTTTGCACATCCATTGACACCATAGAAATGGACCAAAATGTAAAGGATGGTGATCTTCTGGTGTCCAAGGAAAACATCTTCGCATTAGGGTTCTTCAGCCCCAGAAACTCCAGCTCCAGGTACGTCGGAATTTGGTATGCTCAAAAAGATGAAAAAGATCAAAAGGCAGTGGTTTGGGTAGCAAACAGAAACGACCCCATCAATGATACCTCCGGTGTCCTAACTATAGACAGGTATGGGAGACTGCTTCTTTATTCTAACAATATGCAGAACGTTCCTGTGTGGTCTACAAATGTGACGGTTCAAACTGCGAGCACTAGTTGCAGGGCTCAGCTTTTAGATTCTGGAAATTTAGTTCTTTTCCGGGATAATAAAAGCAAAAATTTTATGTGGCAGAGTTTTGATTATCCTACAGATACTCTCCTTCCGGGTATGAAACTAGGCGTGAATTGGAAATTGGGGATAGAATGGGTCTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGAATACACCTATAGGCTCTATTCCAATCAGACTGCCACCCCCGAAGTTTTAACTTTTTATAAAGGTTTGACTCCGCATTGGCGAGCTGATTTAGTGGACGTGGGGCGATCTGATCCACTGGACGTGGGGCGATCTGATCCACTGGACGTGGTTACTTTTGTCAGTAATCAGGACGAAATGTATTACTTTCTTAAAAACGATAGCACTTCAACAAGGGTGGTGTTGAAGGATTCTGGGTTACTGCAACACCTCAGGTGGAACAATGCGGATAGAGAATGGAAGGAACTATGGTCTACGCCAAAATATCGGTGTGACCAGTATGGAGAGTGCGGTGCCAACAGCAAATGTAGCCCTGACAATATCAATTCGTTTGAGTGTGAGTGTCTGCCAGGGTATGAACCGAAGTCTGTCAATGATTGGAAGATGAGAGATGGTTCGGACGGGTGTGTGAGTAAACGACTTGCGGTATCAAAGTGTAGGAACGGAGAAGGGTTCATGAAAGTGGCACGAGTTAAAGATCCAGACACAACCAAAGCAGCACGGCTGAAAAAAGGTATTAGTATCAAAGAGTGTGAGCAGATGTGCTTAAGCAATTGTTCTTGCACTGCATATACGGTCATAGAATCTGAAGGGCGCAGTGATTGCTTGACATGGTATGGTGAGTTGCTGGACATTTTAGTGTACACACAGCTAGGGCGAGATCTACATGTACGTGTGGACAAAATCGAATTAGAAAATTCAAGAAATTCGAAAGGTTTTCTTAGAAGCAGGGGTATGCTGGCTATTCTGATGTTGTCTGTTGTTCTAGCATTGGTACTGATGGTTGCGTTGACCTGTTGGAGGATTAGGAAACAGAGGAAGACAAAAGTTGAGATCGAAGAAACTAGGAGACATCCCGAATTGCAATTTTTCCATCTGAGAACGATAATTGCAGCTACGAACAACTTCTCTCCCGTCCAAAAACTTGGCCAAGGTGGTTTTGGCACTGTTTATAAGGGTGTGTTACGAAATAATCAGAATATTGCTGTAAAAAGATTATCCAGAGCTACAGGACAAGGAGTTGAAGAATTCAAAAATGAAGTTGCATTGATAGCGAGACTTCAACACAGGAATCTTGTGAAACTTTTAGGCTGTTGCATAAAGGGAGAAGAAAGGATGTTAGTCCTGGAATACTTGCCGAACAAAAGCTTGGACTACTTTCTTTTCGATCACACAAAAAAGTCCTTGCTGGATTGGCAAAAGCGATTCGAAATCATCAACGGGGTTGCTCGTGGGGTTTTGTATCTTCACCAGGATTCAAGACTTAGGATTATTCATAGGGATCTAAAAACCAGCAATGTCCTTCTAGACGCTGAGATGAACCCAAAAATCTCAGATTTCGGCATGGCAAGAATCTTCCATGGGGACCAACTCCAGGATACGACCAACAGAGTTGTCGGAACATATGGCTATATGTCACCGGAGTATGTAGTGTTTGGGAGATATTCAACCAAATCAGATGTTTTTAGTTTTGGGGTCATATTATTGGAGATTGCAAGCGGCAAGAAAAACAGCAGTTGTTATCAAGAGGATCATTTCGTAAACTTAATAGGACATGTTTGGCAGTTGTGGAGTGAAGACAGAGCCTTAGAAATTGTGGATTCGTCATTGGAGTCATACGCGCTCGACGAAGCCATGAGATGCATTCAGGTCGGGCTCTTGTGTGTCGAAGAAGAGTCGAAGAATAGACCATCCATGTCAGCTGTTGTTTTTATGTTGAGCGGCGAAGCATCTGCTCCATCTCCTCAGCAGCCTGCATTTGCCTTGAGAAAAAATTCATGCGGCGATGCTGTTGTTCCTTCAGTTTCTGAAGGATCGTGTTCTATCAACGACGATTATTCCCAAGCTGCCACGTGGCAGAAACCCAAGACTTCTCTCTTTTCTCTCTCTGTCCCCTGCCACTCCCGTGAACCCCCATTTTCTTCCTCTTTCTGTCTCTCCCTTCTCTCCCTTTCGGATCCCTCATTTCTCTCTCTCTCTTTTCCGAGACACACATACACACACACACACACACACCCAGTTACCTCATCTACGAGGAAGACGCCGCCATCATCAATCCCTCGTCCTTCTTTCTCTCTCTTTCCCGTGCTACGAGGAAGAACCCAGAAACGAACTCTGGCGAGATTTCTATTCCTCCGGTCGGGTGAAGGCACACCTAGCTCCGGCGAGGCCGTGGGTGTCGAGGGAATCTCCGGTCATCTCCGGCCGTTCTACGGCAAAAGGAAGGTTTGGCAGTTGTGGAGTGAAGACAGAGCCTTAGAAATTGTGGATTTGTCATTGGAGTCATACGCGCCCGACGAAGCCATGAGATGCATCCAGGTTGGGCTCTTGTGTGTTGAAGAAGAGTCGAAGAACAGACAATCCATGTCAGCTGTTGTTTTTATGTTGAGCGGCGAAGATCTGCTCCATCTCCTCAGCAACCCGCATTTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

1054

Amino Acids

118.94

Weight (kDa)

8.45

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 115 - 221 1.6e-35 D-mannose binding lectin
S_locus_glycop PF00954 283 - 365 2.4e-18 S-locus glycoprotein domain
PAN_2 PF08276 388 - 454 3e-17 PAN-like domain
Pkinase PF00069 547 - 810 8.3e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 549 - 812 2.5e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 747, 1680
AccB1I GGYRCC 1 cut(s) 1019
AccBSI CCGCTC 2 cut(s) 2448, 3117
AccI GTMKAC 2 cut(s) 461, 980
AccII CGCG 2 cut(s) 2348, 3017
AclI AACGTT 1 cut(s) 447
AclWI GGATC 8 cut(s) 794, 818, 1199, 2016, 2265, 2548, 2678, 2691
AcoI YGGCCR 2 cut(s) 1654, 2930
AcsI RAATTY 7 cut(s) 303, 523, 556, 1423, 1432, 1754, 2500
AcuI CTGAAG 4 cut(s) 253, 1335, 2511, 2556
AcvI CACGTG 1 cut(s) 2580
AcyI GRCGYC 1 cut(s) 2768
AfaI GTAC 4 cut(s) 296, 1370, 1398, 1503
AfiI CCNNNNNNNGG 7 cut(s) 291, 683, 1527, 2101, 2866, 2888, 2939
AflII CTTAAG 1 cut(s) 1273
AflIII ACRYGT 3 cut(s) 1393, 1399, 2281
AhlI ACTAGT 1 cut(s) 491
AjiI CACGTC 3 cut(s) 787, 811, 835
AjnI CCWGG 7 cut(s) 290, 676, 1075, 1851, 1977, 2100, 3042
AjuI GAANNNNNNNTTGG 4 cut(s) 614, 646, 1859, 1891
AleI CACNNNNGTG 1 cut(s) 2631
AloI GAACNNNNNNTCC 4 cut(s) 513, 545, 3071, 3103
Alw21I GWGCWC 1 cut(s) 491
Alw26I GTCTC 5 cut(s) 409, 1570, 1777, 2666, 2707
AlwI GGATC 8 cut(s) 794, 818, 1199, 2016, 2265, 2548, 2678, 2691
AlwNI CAGNNNCTG 2 cut(s) 90, 2534
AoxI GGCC 4 cut(s) 51, 1654, 2895, 2930
ApeKI GCWGC 4 cut(s) 1223, 1619, 2476, 2573
ApoI RAATTY 7 cut(s) 303, 523, 556, 1423, 1432, 1754, 2500
Asp700I GAANNNNTTC 2 cut(s) 1631, 2500
AspLEI GCGC 3 cut(s) 1323, 2350, 3019
AspS9I GGNCC 3 cut(s) 51, 205, 2092
AsuC2I CCSGG 2 cut(s) 539, 603
AsuHPI GGTGA 5 cut(s) 236, 1358, 1967, 2136, 2882
AsuII TTCGAA 2 cut(s) 1436, 1935
AvaII GGWCC 2 cut(s) 205, 2092
AxyI CCTNAGG 1 cut(s) 938
BalI TGGCCA 1 cut(s) 1656
BamHI GGATCC 1 cut(s) 2683
BanI GGYRCC 1 cut(s) 1019
BanII GRGCYC 3 cut(s) 505, 2385, 3054
BauI CACGAG 2 cut(s) 1194, 1956
BbrPI CACGTG 1 cut(s) 2580
BbsI GAAGAC 4 cut(s) 1553, 2310, 2771, 2979
Bbv12I GWGCWC 1 cut(s) 491
BbvCI CCTCAGC 2 cut(s) 2472, 3140
BbvI GCAGC 4 cut(s) 1235, 1631, 2488, 2560
BccI CCATC 9 cut(s) 215, 380, 1112, 1501, 1608, 2425, 2473, 2782, 3141
BceAI ACGGC 4 cut(s) 1244, 2882, 2917, 2956
BcgI CGANNNNNNTGC 4 cut(s) 2359, 2393, 2441, 2475
BciT130I CCWGG 7 cut(s) 292, 678, 1077, 1853, 1979, 2102, 3044
BciVI GTATCC 1 cut(s) 2098
BcnI CCSGG 2 cut(s) 539, 603
BcoDI GTCTC 5 cut(s) 409, 1570, 1777, 2666, 2707
BcuI ACTAGT 1 cut(s) 491
BfaI CTAG 7 cut(s) 492, 614, 1379, 1493, 1572, 2033, 2883
BfmI CTRYAG 4 cut(s) 399, 585, 697, 1734
BfrI CTTAAG 1 cut(s) 1273
BfuI GTATCC 1 cut(s) 2098
BglII AGATCT 2 cut(s) 1387, 3124
BisI GCNGC 9 cut(s) 1224, 1620, 2227, 2449, 2477, 2509, 2574, 2771, 3118
BlpI GCTNAGC 1 cut(s) 504
BlsI GCNGC 9 cut(s) 1225, 1621, 2228, 2450, 2478, 2510, 2575, 2772, 3119
Bme1390I CCNGG 9 cut(s) 292, 539, 603, 678, 1077, 1853, 1979, 2102, 3044
Bme18I GGWCC 2 cut(s) 205, 2092
BmgBI CACGTC 3 cut(s) 787, 811, 835
BmgT120I GGNCC 3 cut(s) 51, 205, 2092
BmiI GGNNCC 3 cut(s) 1021, 2093, 2685
BmrFI CCNGG 9 cut(s) 292, 539, 603, 678, 1077, 1853, 1979, 2102, 3044
BmrI ACTGGG 2 cut(s) 693, 2738
BmsI GCATC 7 cut(s) 77, 128, 2357, 2465, 2503, 3026, 3048
BmuI ACTGGG 2 cut(s) 693, 2738
BpiI GAAGAC 4 cut(s) 1553, 2310, 2771, 2979
BplI GAGNNNNNCTC 2 cut(s) 2900, 2932
BpmI CTGGAG 3 cut(s) 268, 274, 2084
Bpu10I CCTNAGC 2 cut(s) 2472, 3140
Bpu1102I GCTNAGC 1 cut(s) 504
Bpu14I TTCGAA 2 cut(s) 1436, 1935
BpuEI CTTGAG 1 cut(s) 2512
BpuMI CCSGG 2 cut(s) 539, 603
BsaAI YACGTR 2 cut(s) 1400, 2580
BsaBI GATNNNNATC 1 cut(s) 225
BsaHI GRCGYC 1 cut(s) 2768
BsaJI CCNNGG 7 cut(s) 102, 240, 676, 1076, 1657, 2086, 2898
BsaWI WCCGGW 4 cut(s) 386, 2146, 2862, 2918
BsaXI ACNNNNNCTCC 2 cut(s) 272, 302
Bsc4I CCNNNNNNNGG 7 cut(s) 291, 683, 1527, 2101, 2866, 2888, 2939
Bse1I ACTGG 5 cut(s) 688, 810, 834, 1003, 2744
Bse21I CCTNAGG 1 cut(s) 938
Bse3DI GCAATG 1 cut(s) 2029
Bse8I GATNNNNATC 1 cut(s) 225
BseBI CCWGG 7 cut(s) 292, 678, 1077, 1853, 1979, 2102, 3044
BseDI CCNNGG 7 cut(s) 102, 240, 676, 1076, 1657, 2086, 2898
BseGI GGATG 6 cut(s) 182, 226, 1579, 1848, 2417, 3039
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 7 cut(s) 291, 683, 1527, 2101, 2866, 2888, 2939
BseMI GCAATG 1 cut(s) 2029
BseMII CTCAG 8 cut(s) 97, 518, 952, 1595, 2031, 2073, 2486, 3154
BseNI ACTGG 5 cut(s) 688, 810, 834, 1003, 2744
BseRI GAGGAG 2 cut(s) 2460, 3128
BseX3I CGGCCG 1 cut(s) 2930
BseXI GCAGC 4 cut(s) 1235, 1631, 2488, 2560
Bsh1236I CGCG 2 cut(s) 2348, 3017
Bsh1285I CGRYCG 2 cut(s) 2867, 2933
BshFI GGCC 4 cut(s) 53, 1656, 2897, 2932
BshNI GGYRCC 1 cut(s) 1019
BsiEI CGRYCG 2 cut(s) 2867, 2933
BsiHKAI GWGCWC 1 cut(s) 491
BsiSI CCGG 8 cut(s) 387, 538, 602, 2147, 2863, 2889, 2919, 2929
BslFI GGGAC 2 cut(s) 2105, 2603
BslI CCNNNNNNNGG 7 cut(s) 291, 683, 1527, 2101, 2866, 2888, 2939
BsmAI GTCTC 5 cut(s) 409, 1570, 1777, 2666, 2707
BsmFI GGGAC 2 cut(s) 2105, 2603
BsmI GAATGC 1 cut(s) 2370
BsnI GGCC 4 cut(s) 53, 1656, 2897, 2932
Bsp119I TTCGAA 2 cut(s) 1436, 1935
Bsp1286I GDGCHC 4 cut(s) 491, 505, 2385, 3054
Bsp1407I TGTACA 1 cut(s) 1368
Bsp1720I GCTNAGC 1 cut(s) 504
Bsp19I CCATGG 1 cut(s) 2086
BspANI GGCC 4 cut(s) 53, 1656, 2897, 2932
BspCNI CTCAG 8 cut(s) 96, 517, 951, 1596, 2032, 2072, 2485, 3153
BspFNI CGCG 2 cut(s) 2348, 3017
BspHI TCATGA 1 cut(s) 1182
BspLI GGNNCC 3 cut(s) 1021, 2093, 2685
BspPI GGATC 8 cut(s) 794, 818, 1199, 2016, 2265, 2548, 2678, 2691
BspT104I TTCGAA 2 cut(s) 1436, 1935
BspT107I GGYRCC 1 cut(s) 1019
BspTI CTTAAG 1 cut(s) 1273
BsrBI CCGCTC 2 cut(s) 2448, 3117
BsrDI GCAATG 1 cut(s) 2029
BsrGI TGTACA 1 cut(s) 1368
BsrI ACTGG 5 cut(s) 688, 810, 834, 1003, 2744
BssECI CCNNGG 7 cut(s) 102, 240, 676, 1076, 1657, 2086, 2898
BssNI GRCGYC 1 cut(s) 2768
BssSI CACGAG 2 cut(s) 1194, 1956
BssT1I CCWWGG 4 cut(s) 102, 240, 1657, 2086
Bst2BI CACGAG 2 cut(s) 1194, 1956
Bst2UI CCWGG 7 cut(s) 292, 678, 1077, 1853, 1979, 2102, 3044
Bst4CI ACNGT 3 cut(s) 475, 1303, 1675
Bst6I CTCTTC 2 cut(s) 2394, 3063
BstACI GRCGYC 1 cut(s) 2768
BstAFI CTTAAG 1 cut(s) 1273
BstAPI GCANNNNNTGC 2 cut(s) 495, 1329
BstAUI TGTACA 1 cut(s) 1368
BstBAI YACGTR 2 cut(s) 1400, 2580
BstBI TTCGAA 2 cut(s) 1436, 1935
BstC8I GCNNGC 5 cut(s) 487, 771, 1467, 2224, 2481
BstDSI CCRYGG 2 cut(s) 2086, 2898
BstENI CCTNNNNNAGG 1 cut(s) 1525
BstF5I GGATG 6 cut(s) 182, 226, 1579, 1848, 2417, 3039
BstFNI CGCG 2 cut(s) 2348, 3017
BstHHI GCGC 3 cut(s) 1323, 2350, 3019
BstMAI GTCTC 5 cut(s) 409, 1570, 1777, 2666, 2707
BstMCI CGRYCG 2 cut(s) 2867, 2933
BstMWI GCNNNNNNNGC 6 cut(s) 495, 1035, 1329, 1778, 1921, 2883
BstNI CCWGG 7 cut(s) 292, 678, 1077, 1853, 1979, 2102, 3044
BstNSI RCATGY 3 cut(s) 119, 1397, 2285
BstSCI CCNGG 9 cut(s) 290, 537, 601, 676, 1075, 1851, 1977, 2100, 3042
BstSFI CTRYAG 4 cut(s) 399, 585, 697, 1734
BstUI CGCG 2 cut(s) 2348, 3017
BstV1I GCAGC 4 cut(s) 1235, 1631, 2488, 2560
BstV2I GAAGAC 4 cut(s) 1553, 2310, 2771, 2979
BstX2I RGATCY 5 cut(s) 1204, 1387, 2008, 2683, 3124
BstXI CCANNNNNNTGG 3 cut(s) 48, 202, 1652
BstYI RGATCY 5 cut(s) 1204, 1387, 2008, 2683, 3124
BstZI CGGCCG 1 cut(s) 2930
Bsu36I CCTNAGG 1 cut(s) 938
BsuI GTATCC 1 cut(s) 2098
BsuRI GGCC 4 cut(s) 53, 1656, 2897, 2932
BtgI CCRYGG 2 cut(s) 2086, 2898
BtgZI GCGATG 1 cut(s) 2526
BtrI CACGTC 3 cut(s) 787, 811, 835
BtsCI GGATG 6 cut(s) 182, 226, 1579, 1848, 2417, 3039
BtsI GCAGTG 3 cut(s) 348, 1290, 1330
BtsIMutI CAGTG 6 cut(s) 348, 803, 827, 1290, 1330, 1671
Cac8I GCNNGC 5 cut(s) 487, 771, 1467, 2224, 2481
CaiI CAGNNNCTG 2 cut(s) 90, 2534
CciI TCATGA 1 cut(s) 1182
CfoI GCGC 3 cut(s) 1323, 2350, 3019
Cfr13I GGNCC 3 cut(s) 51, 205, 2092
CseI GACGC 2 cut(s) 2045, 2776
Csp6I GTAC 4 cut(s) 295, 1369, 1397, 1502
CspCI CAANNNNNGTGG 2 cut(s) 885, 920
CviQI GTAC 4 cut(s) 295, 1369, 1397, 1502
EaeI YGGCCR 2 cut(s) 1654, 2930
EagI CGGCCG 1 cut(s) 2930
Eam1104I CTCTTC 2 cut(s) 2394, 3063
EarI CTCTTC 2 cut(s) 2394, 3063
EclXI CGGCCG 1 cut(s) 2930
Eco130I CCWWGG 4 cut(s) 102, 240, 1657, 2086
Eco24I GRGCYC 3 cut(s) 505, 2385, 3054
Eco47I GGWCC 2 cut(s) 205, 2092
Eco52I CGGCCG 1 cut(s) 2930
Eco57I CTGAAG 4 cut(s) 253, 1335, 2511, 2556
Eco72I CACGTG 1 cut(s) 2580
Eco81I CCTNAGG 1 cut(s) 938
EcoNI CCTNNNNNAGG 1 cut(s) 1525
EcoRI GAATTC 1 cut(s) 1754
EcoRII CCWGG 7 cut(s) 290, 676, 1075, 1851, 1977, 2100, 3042
EcoT14I CCWWGG 4 cut(s) 102, 240, 1657, 2086
EcoT22I ATGCAT 3 cut(s) 121, 2372, 3041
EcoT38I GRGCYC 3 cut(s) 505, 2385, 3054
ErhI CCWWGG 4 cut(s) 102, 240, 1657, 2086
FalI AAGNNNNNCTT 4 cut(s) 135, 167, 1431, 1463
FaqI GGGAC 2 cut(s) 2105, 2603
FauI CCCGC 1 cut(s) 3156
FauNDI CATATG 1 cut(s) 2131
FblI GTMKAC 2 cut(s) 461, 980
Fnu4HI GCNGC 9 cut(s) 1224, 1620, 2227, 2449, 2477, 2509, 2574, 2771, 3118
FokI GGATG 6 cut(s) 169, 233, 1566, 1855, 2404, 3026
FriOI GRGCYC 3 cut(s) 505, 2385, 3054
Fsp4HI GCNGC 9 cut(s) 1224, 1620, 2227, 2449, 2477, 2509, 2574, 2771, 3118
FspBI CTAG 7 cut(s) 492, 614, 1379, 1493, 1572, 2033, 2883
GlaI GCGC 3 cut(s) 1322, 2349, 3018
GluI GCNGC 9 cut(s) 1224, 1620, 2227, 2449, 2477, 2509, 2574, 2771, 3118
GsuI CTGGAG 3 cut(s) 268, 274, 2084
HaeIII GGCC 4 cut(s) 53, 1656, 2897, 2932
HapII CCGG 8 cut(s) 387, 538, 602, 2147, 2863, 2889, 2919, 2929
HgaI GACGC 2 cut(s) 2045, 2776
HhaI GCGC 3 cut(s) 1323, 2350, 3019
Hin1I GRCGYC 1 cut(s) 2768
Hin6I GCGC 3 cut(s) 1321, 2348, 3017
HinP1I GCGC 3 cut(s) 1321, 2348, 3017
HincII GTYRAC 1 cut(s) 1518
HindII GTYRAC 1 cut(s) 1518
HindIII AAGCTT 2 cut(s) 144, 1873
HpaII CCGG 8 cut(s) 387, 538, 602, 2147, 2863, 2889, 2919, 2929
HphI GGTGA 5 cut(s) 236, 1358, 1967, 2136, 2882
Hpy99I CGWCG 4 cut(s) 302, 2357, 2561, 3026
HpyCH4III ACNGT 3 cut(s) 475, 1303, 1675
HpyCH4IV ACGT 7 cut(s) 297, 447, 786, 810, 834, 1399, 2579
HpyF10VI GCNNNNNNNGC 6 cut(s) 495, 1035, 1329, 1778, 1921, 2883
HpySE526I ACGT 7 cut(s) 297, 447, 786, 810, 834, 1399, 2579
Hsp92I GRCGYC 1 cut(s) 2768
HspAI GCGC 3 cut(s) 1321, 2348, 3017
LmnI GCTCC 4 cut(s) 293, 2467, 2891, 3135
Lsp1109I GCAGC 4 cut(s) 1235, 1631, 2488, 2560
LweI GCATC 7 cut(s) 77, 128, 2357, 2465, 2503, 3026, 3048
MaeI CTAG 7 cut(s) 492, 614, 1379, 1493, 1572, 2033, 2883
MaeII ACGT 7 cut(s) 297, 447, 786, 810, 834, 1399, 2579
MaeIII GTNAC 9 cut(s) 135, 469, 663, 838, 924, 998, 1689, 2142, 2746
MbiI CCGCTC 2 cut(s) 2448, 3117
MfeI CAATTG 1 cut(s) 1279
MflI RGATCY 5 cut(s) 1204, 1387, 2008, 2683, 3124
MhlI GDGCHC 4 cut(s) 491, 505, 2385, 3054
MlsI TGGCCA 1 cut(s) 1656
MluNI TGGCCA 1 cut(s) 1656
MlyI GAGTC 5 cut(s) 751, 2348, 2411, 3017, 3080
MmeI TCCRAC 3 cut(s) 280, 1505, 2104
Mox20I TGGCCA 1 cut(s) 1656
Mph1103I ATGCAT 3 cut(s) 121, 2372, 3041
MroXI GAANNNNTTC 2 cut(s) 1631, 2500
MscI TGGCCA 1 cut(s) 1656
MseI TTAA 6 cut(s) 650, 737, 879, 1200, 1274, 2273
MslI CAYNNNNRTG 6 cut(s) 85, 200, 1187, 1364, 1398, 2631
Msp20I TGGCCA 1 cut(s) 1656
MspA1I CMGCKG 2 cut(s) 2429, 3098
MspCI CTTAAG 1 cut(s) 1273
MspI CCGG 8 cut(s) 387, 538, 602, 2147, 2863, 2889, 2919, 2929
MspR9I CCNGG 9 cut(s) 292, 539, 603, 678, 1077, 1853, 1979, 2102, 3044
MunI CAATTG 1 cut(s) 1279
Mva1269I GAATGC 1 cut(s) 2370
MvaI CCWGG 7 cut(s) 292, 678, 1077, 1853, 1979, 2102, 3044
MvnI CGCG 2 cut(s) 2348, 3017
MwoI GCNNNNNNNGC 6 cut(s) 495, 1035, 1329, 1778, 1921, 2883
NciI CCSGG 2 cut(s) 539, 603
NcoI CCATGG 1 cut(s) 2086
NdeI CATATG 1 cut(s) 2131
NlaIV GGNNCC 3 cut(s) 1021, 2093, 2685
NmuCI GTSAC 4 cut(s) 469, 663, 998, 2142
NsiI ATGCAT 3 cut(s) 121, 2372, 3041
NspI RCATGY 3 cut(s) 119, 1397, 2285
NspV TTCGAA 2 cut(s) 1436, 1935
OliI CACNNNNGTG 1 cut(s) 2631
PagI TCATGA 1 cut(s) 1182
PciI ACATGT 2 cut(s) 1393, 2281
PcsI WCGNNNNNNNCGW 2 cut(s) 2352, 3021
PctI GAATGC 1 cut(s) 2370
PdmI GAANNNNTTC 2 cut(s) 1631, 2500
PfoI TCCNGGA 3 cut(s) 537, 1851, 2100
PkrI GCNGC 9 cut(s) 1225, 1621, 2228, 2450, 2478, 2510, 2575, 2772, 3119
PleI GAGTC 5 cut(s) 751, 2347, 2410, 3016, 3079
PmaCI CACGTG 1 cut(s) 2580
PmlI CACGTG 1 cut(s) 2580
PpsI GAGTC 5 cut(s) 751, 2347, 2410, 3016, 3079
Ppu21I YACGTR 2 cut(s) 1400, 2580
PscI ACATGT 2 cut(s) 1393, 2281
PsiI TTATAA 2 cut(s) 747, 1680
Psp1406I AACGTT 1 cut(s) 447
Psp6I CCWGG 7 cut(s) 290, 676, 1075, 1851, 1977, 2100, 3042
PspCI CACGTG 1 cut(s) 2580
PspGI CCWGG 7 cut(s) 290, 676, 1075, 1851, 1977, 2100, 3042
PspN4I GGNNCC 3 cut(s) 1021, 2093, 2685
PspPI GGNCC 3 cut(s) 51, 205, 2092
PstNI CAGNNNCTG 2 cut(s) 90, 2534
PsuI RGATCY 5 cut(s) 1204, 1387, 2008, 2683, 3124
PvuII CAGCTG 2 cut(s) 2429, 3098
RsaI GTAC 4 cut(s) 296, 1370, 1398, 1503
RsaNI GTAC 4 cut(s) 295, 1369, 1397, 1502
RseI CAYNNNNRTG 6 cut(s) 85, 200, 1187, 1364, 1398, 2631
SaqAI TTAA 6 cut(s) 650, 737, 879, 1200, 1274, 2273
SatI GCNGC 9 cut(s) 1224, 1620, 2227, 2449, 2477, 2509, 2574, 2771, 3118
Sau96I GGNCC 3 cut(s) 51, 205, 2092
SchI GAGTC 5 cut(s) 751, 2348, 2411, 3017, 3080
ScrFI CCNGG 9 cut(s) 292, 539, 603, 678, 1077, 1853, 1979, 2102, 3044
SduI GDGCHC 4 cut(s) 491, 505, 2385, 3054
SfaNI GCATC 7 cut(s) 77, 128, 2357, 2465, 2503, 3026, 3048
SfcI CTRYAG 4 cut(s) 399, 585, 697, 1734
SfuI TTCGAA 2 cut(s) 1436, 1935
SinI GGWCC 2 cut(s) 205, 2092
SmiMI CAYNNNNRTG 6 cut(s) 85, 200, 1187, 1364, 1398, 2631
SmlI CTYRAG 2 cut(s) 1273, 2491
SmoI CTYRAG 2 cut(s) 1273, 2491
SpeI ACTAGT 1 cut(s) 491
SspI AATATT 1 cut(s) 1708
SspMI CTAG 7 cut(s) 492, 614, 1379, 1493, 1572, 2033, 2883
StyD4I CCNGG 9 cut(s) 290, 537, 601, 676, 1075, 1851, 1977, 2100, 3042
StyI CCWWGG 4 cut(s) 102, 240, 1657, 2086
TaaI ACNGT 3 cut(s) 475, 1303, 1675
TaiI ACGT 7 cut(s) 300, 450, 789, 813, 837, 1402, 2582
TatI WGTACW 1 cut(s) 1368
TauI GCSGC 5 cut(s) 2229, 2451, 2511, 2773, 3120
Tru1I TTAA 6 cut(s) 650, 737, 879, 1200, 1274, 2273
Tru9I TTAA 6 cut(s) 650, 737, 879, 1200, 1274, 2273
TscAI CASTG 6 cut(s) 348, 810, 834, 1297, 1330, 1678
TseFI GTSAC 4 cut(s) 469, 663, 998, 2142
TseI GCWGC 4 cut(s) 1223, 1619, 2476, 2573
Tsp45I GTSAC 4 cut(s) 469, 663, 998, 2142
TspDTI ATGAA 9 cut(s) 339, 623, 1098, 1171, 1199, 1779, 1991, 2060, 2493
TspGWI ACGGA 1 cut(s) 1185
TspRI CASTG 6 cut(s) 348, 810, 834, 1297, 1330, 1678
Vha464I CTTAAG 1 cut(s) 1273
VpaK11BI GGWCC 2 cut(s) 205, 2092
XagI CCTNNNNNAGG 1 cut(s) 1525
XapI RAATTY 7 cut(s) 303, 523, 556, 1423, 1432, 1754, 2500
XbaI TCTAGA 1 cut(s) 2032
XceI RCATGY 3 cut(s) 119, 1397, 2285
XcmI CCANNNNNNNNNTGG 1 cut(s) 1664
XmiI GTMKAC 2 cut(s) 461, 980
XmnI GAANNNNTTC 2 cut(s) 1631, 2500
XspI CTAG 7 cut(s) 492, 614, 1379, 1493, 1572, 2033, 2883
Zsp2I ATGCAT 3 cut(s) 121, 2372, 3041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.