FvH4_6g20800

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000112
Physical Location & Seq
Forward (+)
14330055 .. 14334854
4800 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g20800.t3

Sequence Viewer

Length: 2436 bp
ATGCCATTGAAATCTTTGCTTCTCTTCCTCCTCTTCCAACTTTGCACTTCCAGGGACACCTTGAGATCGAACCAAGAAAGTCTGAGGGATGATGATGGTGGGCTTCTAGTGTCCAAAGAAAGAAACTTTGAGTTGGGTTTCTTCAGCCCCGGAAATTCCAGCAACCGCTATGTTGGGATTTGGTTTTCTCAATCTAGAGTGTCTGAAAAGACAGTGGTTTGGATTGCAAACAGAGACAACTCCATCAATGATACCTCTGGAGTTTTCACTGTAAACAGGTACGGAGAGTTAGTGCTTTATGCTTATAATATGGAGACTAGTCCTATATGGTCTGCAAATGTGTCTCATTTAGTCAAAGACAAAAGCACCTTATCAGCATATGTTCAGCTATTGGATACAGGAAATTTAGTTATGTTCCAGAATGATAGGGATGGAATCTTTGTATGGCAGAGTTTTGATTATCCTACAGATACTCTAATCCCTGGTATGAAAGTTGGGATGAATTGGAAAACTGGGTTAGAATGGGTTTTAACATCTTGGAAATCACAAGATGACCCTGGCACTGGGGACTATACCTTTAGGCTTCATAGTTGGAACCGCATTGCCCTATCCCAGTATTTTATTTATAAAAATTTGTCCAAGTATTGGCGAAGTGATCCAGACCCATGGCCGAATTCGGTCAGAAATGCAGAGGAAATGTCTTATCTACTAACTTATAACACCGATAAACTTACAATAATGAAGTTGAAGTTTTCTGGGATACATCAGCACTTAGTATGGAACGATGGTGACCATCTATGGAAGGAGCTGTGGTCGTCACCGAAATACCGGTGTGACTGGTATGGACATTGTGGTGCCAACAGCAAATGTAGCCCTGACAATGGTAATCTGTTTGAGTGCGAGTGTTTACCGGGGTATGAACCAAATTCTATAAATGGTTGGAATGGAAGAGATGGGTCAGATGGATGCGTGAGTAAACGAATCGGAGTGTCAAAGTGTAGAAATGGAGAAGGGTTTGTGAAGGTGGCAAGGGTGAAAGATCCAGACGCATCGAGAGCAGCATTGTTGAAAACAAGTATGAGTGCCAAAGAGTGTGAGCAGGAGTGCTTAAGAAATTGTTCTTGCACGGCATATATGAGCAAAAAGATTGAAGGGGTTGTCGATTGCTTGACATGGTATGATGACTTGATGGACATTTTGGTTTACACCGAAACCGGACGAGATCTATATGTTCGTGTAGATAAAATCGAGTTAGCTGAGAATGCTGCAAACTCACAAGGATTCTTCAAGAGGGGCATGCTTGTTAGTCCAATACTGTTTGCTTTACTGGCCTTGGTACCAATCAATGTGCTTGCCTGTTGGTTGCTTAAGAAAAAAGGGAAGACAAAAGACTTTGTGGAGGTGGATGAACTTGAGGAAAGCAAGAGACACCCGGAATTGCAATTTTTCGATCTGAACACACTAATAGCAGCCACAGATAATTTCTCAGACGTTAATAAACTTGGTGAAGGTGGTTTTGGTTGTGTTTATAAGGGTGAGTTGCCAAATGAACAGAAGGTTGCTGTGAAAAGGTTGTCTAGTAGCTCAGGACAAGGGGTTGATGAATTCAAAAATGAAGTTGAACTGATAGCAGGACTTCAACACAGGAACCTTGTGAAGCTTTTAGGTTGTTGTATAAAGGGAGGAGAAAGGATGCTGGTTCTCGAATACATGCCAAACAAAAGCTTGGACTCCTTTCTTTTTGATAACAGTAGAAGGTCCTTCTTAAATTGGGAAAGACGGTTTGACATCATCAACGGGGTTGCTCGTGGGCTTTTGTATCTTCACCAGGATTCCAGATTAAGGATTATTCATAGGGATCTAAAAACCAGCAATATTCTTCTAGATGCTGAGATGACCCCAAAAATTTCAGATTTCGGCATGGCAAGAATCTTTCATGGGGACCAACTTCAGGATAAGACAAAGAAAGTTGTTGGAACATATGGCTACATGTCACCAGAGTATGCAGTGTATGGGAGATTCTCCACGAAATCGGATGTGTTTGGTTTTGGGATTATACTACTGGAGATTATTAGTGGCAAGAGAAATAATGGTTCTTACGAGGATGATTACTTCATGAACCTGATAGGACATGTTTGGCATCTGTGGAAAGAAGGAACAGCCTTAGAAATTCTTGATTCATCATTACAATCATATCAACCTGATGAAGTTTTGAGATGCATTCAAGTTGGACTGTTGTGTGTGCAAGAAGATTCAAAGGACCGGCCAGCCATGTCATCTGTCGTGTTCATGTTGAGCGGTGAGGCATCTCTTCCATCTCCTAATCAGCCTGCATTTGTCTTTGGAAGAAAATCCAGCATTGTTGCTGATCCCTTGATTCCAAAACAATTGTATTCTACTAACGACGTGACAATAACTATTATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

812

Amino Acids

92.07

Weight (kDa)

6.01

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 71 - 181 2.1e-32 D-mannose binding lectin
S_locus_glycop PF00954 228 - 309 1.5e-14 S-locus glycoprotein domain
PAN_2 PF08276 333 - 399 7.4e-17 PAN-like domain
Pkinase PF00069 496 - 702 1.8e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 499 - 762 1.2e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 306, 627, 717, 1530
Acc65I GGTACC 1 cut(s) 1336
AccB1I GGYRCC 2 cut(s) 854, 1336
AccB7I CCANNNNNTGG 1 cut(s) 645
AccBSI CCGCTC 1 cut(s) 2298
AciI CCGC 3 cut(s) 166, 598, 2298
AclWI GGATC 4 cut(s) 650, 1034, 1866, 2363
AcoI YGGCCR 2 cut(s) 668, 2264
AcsI RAATTY 8 cut(s) 154, 403, 631, 673, 925, 1604, 1905, 2169
AcuI CTGAAG 2 cut(s) 127, 1934
AfaI GTAC 2 cut(s) 281, 1338
AfiI CCNNNNNNNGG 5 cut(s) 563, 645, 881, 1842, 1951
AflII CTTAAG 2 cut(s) 1108, 1367
AflIII ACRYGT 2 cut(s) 1989, 2131
AgeI ACCGGT 1 cut(s) 828
AhlI ACTAGT 1 cut(s) 317
AjiI CACGTC 1 cut(s) 2407
AjnI CCWGG 4 cut(s) 50, 481, 556, 1827
AjuI GAANNNNNNNTTGG 2 cut(s) 1500, 1532
AluBI AGCT 7 cut(s) 388, 808, 1256, 1584, 1660, 1725, 2429
AluI AGCT 7 cut(s) 388, 808, 1256, 1584, 1660, 1725, 2429
Alw26I GTCTC 4 cut(s) 228, 308, 348, 1420
AlwI GGATC 4 cut(s) 650, 1034, 1866, 2363
AoxI GGCC 3 cut(s) 668, 1329, 2264
ApeKI GCWGC 3 cut(s) 1058, 1265, 1469
ApoI RAATTY 8 cut(s) 154, 403, 631, 673, 925, 1604, 1905, 2169
AsiGI ACCGGT 1 cut(s) 828
Asp700I GAANNNNTTC 1 cut(s) 1117
Asp718I GGTACC 1 cut(s) 1336
AspS9I GGNCC 3 cut(s) 1758, 1942, 2260
AsuC2I CCSGG 3 cut(s) 150, 912, 1433
AsuHPI GGTGA 8 cut(s) 800, 810, 1045, 1517, 1547, 1817, 1986, 2312
AvaII GGWCC 3 cut(s) 1758, 1942, 2260
BanI GGYRCC 2 cut(s) 854, 1336
BauI CACGAG 1 cut(s) 1806
BbsI GAAGAC 1 cut(s) 1388
BbvI GCAGC 3 cut(s) 1070, 1252, 1481
BccI CCATC 9 cut(s) 89, 251, 425, 779, 801, 947, 956, 1183, 2323
BceAI ACGGC 1 cut(s) 1143
BciT130I CCWGG 4 cut(s) 52, 483, 558, 1829
BciVI GTATCC 2 cut(s) 388, 753
BcnI CCSGG 3 cut(s) 150, 912, 1433
BcoDI GTCTC 4 cut(s) 228, 308, 348, 1420
BcuI ACTAGT 1 cut(s) 317
BfaI CTAG 5 cut(s) 107, 195, 318, 1578, 1883
BfmI CTRYAG 1 cut(s) 465
BfrI CTTAAG 2 cut(s) 1108, 1367
BfuI GTATCC 2 cut(s) 388, 753
BglII AGATCT 1 cut(s) 1222
BisI GCNGC 3 cut(s) 1059, 1266, 1470
BlsI GCNGC 3 cut(s) 1060, 1267, 1471
Bme1390I CCNGG 7 cut(s) 52, 150, 483, 558, 912, 1433, 1829
Bme18I GGWCC 3 cut(s) 1758, 1942, 2260
BmgBI CACGTC 1 cut(s) 2407
BmgT120I GGNCC 3 cut(s) 1758, 1942, 2260
BmiI GGNNCC 5 cut(s) 596, 856, 1338, 1649, 1943
BmrFI CCNGG 7 cut(s) 52, 150, 483, 558, 912, 1433, 1829
BmrI ACTGGG 3 cut(s) 522, 573, 607
BmsI GCATC 7 cut(s) 956, 1058, 1683, 1876, 2149, 2207, 2315
BmuI ACTGGG 3 cut(s) 522, 573, 607
BpiI GAAGAC 1 cut(s) 1388
BpmI CTGGAG 2 cut(s) 279, 2084
Bpu10I CCTNAGC 1 cut(s) 1585
BpuEI CTTGAG 2 cut(s) 82, 1433
BpuMI CCSGG 3 cut(s) 150, 912, 1433
BsaJI CCNNGG 7 cut(s) 51, 148, 481, 556, 665, 911, 1332
BsaWI WCCGGW 2 cut(s) 828, 1214
BsaXI ACNNNNNCTCC 4 cut(s) 276, 306, 797, 827
Bsc4I CCNNNNNNNGG 5 cut(s) 563, 645, 881, 1842, 1951
Bse118I RCCGGY 2 cut(s) 828, 2262
Bse1I ACTGG 6 cut(s) 517, 568, 613, 842, 1332, 2067
Bse3DI GCAATG 1 cut(s) 600
BseBI CCWGG 4 cut(s) 52, 483, 558, 1829
BseDI CCNNGG 7 cut(s) 51, 148, 481, 556, 665, 911, 1332
BseGI GGATG 8 cut(s) 94, 436, 504, 971, 1411, 1698, 2041, 2110
BseLI CCNNNNNNNGG 5 cut(s) 563, 645, 881, 1842, 1951
BseMI GCAATG 1 cut(s) 600
BseMII CTCAG 5 cut(s) 74, 1248, 1500, 1599, 1881
BseNI ACTGG 6 cut(s) 517, 568, 613, 842, 1332, 2067
BseRI GAGGAG 2 cut(s) 20, 1698
BseXI GCAGC 3 cut(s) 1070, 1252, 1481
BshFI GGCC 3 cut(s) 670, 1331, 2266
BshNI GGYRCC 2 cut(s) 854, 1336
BshTI ACCGGT 1 cut(s) 828
BsiSI CCGG 6 cut(s) 150, 829, 911, 1215, 1433, 2263
BslFI GGGAC 3 cut(s) 68, 581, 1955
BslI CCNNNNNNNGG 5 cut(s) 563, 645, 881, 1842, 1951
BsmAI GTCTC 4 cut(s) 228, 308, 348, 1420
BsmFI GGGAC 3 cut(s) 68, 581, 1955
BsmI GAATGC 2 cut(s) 1267, 2220
BsnI GGCC 3 cut(s) 670, 1331, 2266
Bsp143I GATC 7 cut(s) 65, 655, 1039, 1222, 1450, 1858, 2368
Bsp19I CCATGG 1 cut(s) 665
BspACI CCGC 3 cut(s) 166, 598, 2298
BspANI GGCC 3 cut(s) 670, 1331, 2266
BspCNI CTCAG 5 cut(s) 75, 1249, 1499, 1598, 1882
BspHI TCATGA 1 cut(s) 2115
BspLI GGNNCC 5 cut(s) 596, 856, 1338, 1649, 1943
BspPI GGATC 4 cut(s) 650, 1034, 1866, 2363
BspT107I GGYRCC 2 cut(s) 854, 1336
BspTI CTTAAG 2 cut(s) 1108, 1367
BsrBI CCGCTC 1 cut(s) 2298
BsrDI GCAATG 1 cut(s) 600
BsrFI RCCGGY 2 cut(s) 828, 2262
BsrI ACTGG 6 cut(s) 517, 568, 613, 842, 1332, 2067
BssAI RCCGGY 2 cut(s) 828, 2262
BssECI CCNNGG 7 cut(s) 51, 148, 481, 556, 665, 911, 1332
BssMI GATC 7 cut(s) 65, 655, 1039, 1222, 1450, 1858, 2368
BssSI CACGAG 1 cut(s) 1806
BssT1I CCWWGG 2 cut(s) 665, 1332
Bst2BI CACGAG 1 cut(s) 1806
Bst2UI CCWGG 4 cut(s) 52, 483, 558, 1829
Bst4CI ACNGT 6 cut(s) 214, 271, 1317, 1751, 1782, 2235
Bst6I CTCTTC 4 cut(s) 29, 38, 943, 2316
BstAFI CTTAAG 2 cut(s) 1108, 1367
BstC8I GCNNGC 4 cut(s) 1298, 1353, 2268, 2331
BstDEI CTNAG 7 cut(s) 83, 772, 1257, 1486, 1585, 1890, 2164
BstDSI CCRYGG 1 cut(s) 665
BstEII GGTNACC 1 cut(s) 788
BstF5I GGATG 8 cut(s) 94, 436, 504, 971, 1411, 1698, 2041, 2110
BstKTI GATC 7 cut(s) 68, 658, 1042, 1225, 1453, 1861, 2371
BstMAI GTCTC 4 cut(s) 228, 308, 348, 1420
BstMBI GATC 7 cut(s) 65, 655, 1039, 1222, 1450, 1858, 2368
BstMWI GCNNNNNNNGC 4 cut(s) 870, 1055, 1262, 1328
BstNI CCWGG 4 cut(s) 52, 483, 558, 1829
BstNSI RCATGY 4 cut(s) 1300, 1714, 1993, 2135
BstPI GGTNACC 1 cut(s) 788
BstSCI CCNGG 7 cut(s) 50, 148, 481, 556, 910, 1431, 1827
BstSFI CTRYAG 1 cut(s) 465
BstV1I GCAGC 3 cut(s) 1070, 1252, 1481
BstV2I GAAGAC 1 cut(s) 1388
BstX2I RGATCY 3 cut(s) 1039, 1222, 1858
BstXI CCANNNNNNTGG 1 cut(s) 666
BstYI RGATCY 3 cut(s) 1039, 1222, 1858
BsuI GTATCC 2 cut(s) 388, 753
BsuRI GGCC 3 cut(s) 670, 1331, 2266
BtgI CCRYGG 1 cut(s) 665
BtrI CACGTC 1 cut(s) 2407
BtsCI GGATG 8 cut(s) 94, 436, 504, 971, 1411, 1698, 2041, 2110
BtsI GCAGTG 1 cut(s) 2013
BtsIMutI CAGTG 4 cut(s) 219, 267, 561, 2013
Cac8I GCNNGC 4 cut(s) 1298, 1353, 2268, 2331
CciI TCATGA 1 cut(s) 2115
Cfr10I RCCGGY 2 cut(s) 828, 2262
Cfr13I GGNCC 3 cut(s) 1758, 1942, 2260
CseI GACGC 1 cut(s) 1055
Csp6I GTAC 2 cut(s) 280, 1337
CspAI ACCGGT 1 cut(s) 828
CviQI GTAC 2 cut(s) 280, 1337
DdeI CTNAG 7 cut(s) 83, 772, 1257, 1486, 1585, 1890, 2164
DpnI GATC 7 cut(s) 67, 657, 1041, 1224, 1452, 1860, 2370
DpnII GATC 7 cut(s) 65, 655, 1039, 1222, 1450, 1858, 2368
EaeI YGGCCR 2 cut(s) 668, 2264
Eam1104I CTCTTC 4 cut(s) 29, 38, 943, 2316
EarI CTCTTC 4 cut(s) 29, 38, 943, 2316
Eco130I CCWWGG 2 cut(s) 665, 1332
Eco47I GGWCC 3 cut(s) 1758, 1942, 2260
Eco57I CTGAAG 2 cut(s) 127, 1934
Eco91I GGTNACC 1 cut(s) 788
EcoO109I RGGNCCY 1 cut(s) 1758
EcoO65I GGTNACC 1 cut(s) 788
EcoRI GAATTC 2 cut(s) 673, 1604
EcoRII CCWGG 4 cut(s) 50, 481, 556, 1827
EcoT14I CCWWGG 2 cut(s) 665, 1332
EcoT22I ATGCAT 1 cut(s) 2222
ErhI CCWWGG 2 cut(s) 665, 1332
FaqI GGGAC 3 cut(s) 68, 581, 1955
FauNDI CATATG 2 cut(s) 379, 1981
Fnu4HI GCNGC 3 cut(s) 1059, 1266, 1470
FokI GGATG 8 cut(s) 101, 443, 511, 978, 1418, 1705, 2048, 2117
Fsp4HI GCNGC 3 cut(s) 1059, 1266, 1470
FspBI CTAG 5 cut(s) 107, 195, 318, 1578, 1883
GluI GCNGC 3 cut(s) 1059, 1266, 1470
GsuI CTGGAG 2 cut(s) 279, 2084
HaeIII GGCC 3 cut(s) 670, 1331, 2266
HapII CCGG 6 cut(s) 150, 829, 911, 1215, 1433, 2263
HgaI GACGC 1 cut(s) 1055
HindIII AAGCTT 2 cut(s) 1658, 1723
HpaII CCGG 6 cut(s) 150, 829, 911, 1215, 1433, 2263
HphI GGTGA 8 cut(s) 800, 810, 1045, 1517, 1547, 1817, 1986, 2312
Hpy166II GTNNAC 4 cut(s) 274, 908, 977, 1204
Hpy188I TCNGA 9 cut(s) 84, 205, 683, 961, 986, 1455, 1489, 1912, 2035
Hpy8I GTNNAC 4 cut(s) 274, 908, 977, 1204
Hpy99I CGWCG 1 cut(s) 2408
HpyAV CCTTC 9 cut(s) 796, 1004, 1015, 1145, 1502, 1549, 1749, 1771, 2147
HpyCH4III ACNGT 6 cut(s) 214, 271, 1317, 1751, 1782, 2235
HpyCH4IV ACGT 2 cut(s) 1491, 2406
HpyF10VI GCNNNNNNNGC 4 cut(s) 870, 1055, 1262, 1328
HpyF3I CTNAG 7 cut(s) 83, 772, 1257, 1486, 1585, 1890, 2164
HpySE526I ACGT 2 cut(s) 1491, 2406
KpnI GGTACC 1 cut(s) 1340
Kzo9I GATC 7 cut(s) 65, 655, 1039, 1222, 1450, 1858, 2368
LmnI GCTCC 1 cut(s) 805
Lsp1109I GCAGC 3 cut(s) 1070, 1252, 1481
LweI GCATC 7 cut(s) 956, 1058, 1683, 1876, 2149, 2207, 2315
MaeI CTAG 5 cut(s) 107, 195, 318, 1578, 1883
MaeII ACGT 2 cut(s) 1491, 2406
MaeIII GTNAC 5 cut(s) 788, 816, 833, 1992, 2407
MalI GATC 7 cut(s) 67, 657, 1041, 1224, 1452, 1860, 2370
MbiI CCGCTC 1 cut(s) 2298
MboI GATC 7 cut(s) 65, 655, 1039, 1222, 1450, 1858, 2368
MfeI CAATTG 1 cut(s) 2387
MflI RGATCY 3 cut(s) 1039, 1222, 1858
MlyI GAGTC 1 cut(s) 1724
MmeI TCCRAC 5 cut(s) 61, 572, 920, 1954, 2209
Mph1103I ATGCAT 1 cut(s) 2222
MroXI GAANNNNTTC 1 cut(s) 1117
MseI TTAA 6 cut(s) 530, 1109, 1368, 1494, 1766, 1841
MslI CAYNNNNRTG 1 cut(s) 852
MspCI CTTAAG 2 cut(s) 1108, 1367
MspI CCGG 6 cut(s) 150, 829, 911, 1215, 1433, 2263
MspR9I CCNGG 7 cut(s) 52, 150, 483, 558, 912, 1433, 1829
MunI CAATTG 1 cut(s) 2387
Mva1269I GAATGC 2 cut(s) 1267, 2220
MvaI CCWGG 4 cut(s) 52, 483, 558, 1829
MwoI GCNNNNNNNGC 4 cut(s) 870, 1055, 1262, 1328
NciI CCSGG 3 cut(s) 150, 912, 1433
NcoI CCATGG 1 cut(s) 665
NdeI CATATG 2 cut(s) 379, 1981
NdeII GATC 7 cut(s) 65, 655, 1039, 1222, 1450, 1858, 2368
NlaIV GGNNCC 5 cut(s) 596, 856, 1338, 1649, 1943
NmuCI GTSAC 5 cut(s) 788, 816, 833, 1992, 2407
NsiI ATGCAT 1 cut(s) 2222
NspI RCATGY 4 cut(s) 1300, 1714, 1993, 2135
PaeI GCATGC 1 cut(s) 1300
PagI TCATGA 1 cut(s) 2115
PciI ACATGT 2 cut(s) 1989, 2131
PctI GAATGC 2 cut(s) 1267, 2220
PdmI GAANNNNTTC 1 cut(s) 1117
PfeI GAWTC 9 cut(s) 435, 981, 1281, 1832, 1929, 2019, 2177, 2252, 2377
PflMI CCANNNNNTGG 1 cut(s) 645
PinAI ACCGGT 1 cut(s) 828
PkrI GCNGC 3 cut(s) 1060, 1267, 1471
PleI GAGTC 1 cut(s) 1724
PpsI GAGTC 1 cut(s) 1724
PpuMI RGGWCCY 1 cut(s) 1758
PscI ACATGT 2 cut(s) 1989, 2131
PsiI TTATAA 4 cut(s) 306, 627, 717, 1530
Psp5II RGGWCCY 1 cut(s) 1758
Psp6I CCWGG 4 cut(s) 50, 481, 556, 1827
PspEI GGTNACC 1 cut(s) 788
PspGI CCWGG 4 cut(s) 50, 481, 556, 1827
PspN4I GGNNCC 5 cut(s) 596, 856, 1338, 1649, 1943
PspPI GGNCC 3 cut(s) 1758, 1942, 2260
PspPPI RGGWCCY 1 cut(s) 1758
PsuI RGATCY 3 cut(s) 1039, 1222, 1858
RsaI GTAC 2 cut(s) 281, 1338
RsaNI GTAC 2 cut(s) 280, 1337
RseI CAYNNNNRTG 1 cut(s) 852
SaqAI TTAA 6 cut(s) 530, 1109, 1368, 1494, 1766, 1841
SatI GCNGC 3 cut(s) 1059, 1266, 1470
Sau3AI GATC 7 cut(s) 65, 655, 1039, 1222, 1450, 1858, 2368
Sau96I GGNCC 3 cut(s) 1758, 1942, 2260
SchI GAGTC 1 cut(s) 1724
ScrFI CCNGG 7 cut(s) 52, 150, 483, 558, 912, 1433, 1829
SfaNI GCATC 7 cut(s) 956, 1058, 1683, 1876, 2149, 2207, 2315
SfcI CTRYAG 1 cut(s) 465
SinI GGWCC 3 cut(s) 1758, 1942, 2260
SmiMI CAYNNNNRTG 1 cut(s) 852
SmlI CTYRAG 4 cut(s) 61, 1108, 1367, 1412
SmoI CTYRAG 4 cut(s) 61, 1108, 1367, 1412
SpeI ACTAGT 1 cut(s) 317
SphI GCATGC 1 cut(s) 1300
SsiI CCGC 3 cut(s) 166, 598, 2298
SspI AATATT 1 cut(s) 1876
SspMI CTAG 5 cut(s) 107, 195, 318, 1578, 1883
StyD4I CCNGG 7 cut(s) 50, 148, 481, 556, 910, 1431, 1827
StyI CCWWGG 2 cut(s) 665, 1332
TaaI ACNGT 6 cut(s) 214, 271, 1317, 1751, 1782, 2235
TaiI ACGT 2 cut(s) 1494, 2409
TaqI TCGA 7 cut(s) 68, 1052, 1161, 1248, 1449, 1704, 2431
TaqII GACCGA 1 cut(s) 667
TfiI GAWTC 9 cut(s) 435, 981, 1281, 1832, 1929, 2019, 2177, 2252, 2377
Tru1I TTAA 6 cut(s) 530, 1109, 1368, 1494, 1766, 1841
Tru9I TTAA 6 cut(s) 530, 1109, 1368, 1494, 1766, 1841
TscAI CASTG 4 cut(s) 219, 274, 568, 2013
TseFI GTSAC 5 cut(s) 788, 816, 833, 1992, 2407
TseI GCWGC 3 cut(s) 1058, 1265, 1469
Tsp45I GTSAC 5 cut(s) 788, 816, 833, 1992, 2407
TspGWI ACGGA 1 cut(s) 297
TspRI CASTG 4 cut(s) 219, 274, 568, 2013
Van91I CCANNNNNTGG 1 cut(s) 645
Vha464I CTTAAG 2 cut(s) 1108, 1367
VpaK11BI GGWCC 3 cut(s) 1758, 1942, 2260
XapI RAATTY 8 cut(s) 154, 403, 631, 673, 925, 1604, 1905, 2169
XbaI TCTAGA 2 cut(s) 194, 1882
XceI RCATGY 4 cut(s) 1300, 1714, 1993, 2135
XmnI GAANNNNTTC 1 cut(s) 1117
XspI CTAG 5 cut(s) 107, 195, 318, 1578, 1883
Zsp2I ATGCAT 1 cut(s) 2222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.