Rroxscaffold_1G00070590

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000112
Physical Location & Seq
Reverse (-)
91527878 .. 91536775
8898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00070590.1

Sequence Viewer

Length: 2160 bp
ATGAATTATTGTCATCTCAGACTAGTCAAAATGACCCTATTTGGAAAAGTTCAAAGTAGAAGAATTGCCCAGAAGATCAACATAATGCGACTCATCTCATTTATCACCAGCTCAAGTATGAAACTGAGAAAAAATATGCCATTGGTTCATTCCAACAGAATTGAAAATGCCGAGAAAATGGTTTTGAAAGCTCTGCTTCTCTTTCTCTTCTTTCAGTTCTGCACTTCCAGGGACACCATGAGAGGGAACGAAGAACAATTGAAGGATGTTGATGGTGACTATTTAGTGTCCAAAGAAAGCAAGTTTGAATTGGGTTTCTTCAGCCCCGGAAATTCTAGCTACCGGTATGTTGGAATCTGGTATTCTCATACTAGAGTACCTAATAAAACAGTGGTGTGGGTTGCAAACAGGAACAATCCCATCAATGATACCTCTGGTGTGCTCACAATGAACAGGTATGGAGAACTAGTCCTTTATGCTTATAACATGGAGAGCAGTCCTATATGGTCCACAAATGTGTCGCGGTCGGTTCAAAATGTTAACACAAGCACTTTATCTGCACAGCTTTTGGATACAGGAAATTTAGTTGTGTTCCTGGATGATAAAAATGAAATCTTTATATGGCAAAGTTTTGATCATCCTATAGATACTTTAATTCCAGGTATGAAAGTTGGGGTGAATTGGAAAACTGGGCTAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACCTACAGGCTAAGTTCAGATCATAATGCATCTCCCCAAATTTTTTTGTATAAAGGTTTGAGTAAGTATTGGCGAAGTGATCCAGGGCCAGTGCCTACTTTGGTCGCTAATCAAGATGAAACTTATTATTTCATGAATGAAACCAATGCAATTACAAGAATAACAGTGACTGATTCTGCCTTAGAGCACCTTATATGGGATGGTGGTGGCCTTCAATGGAAGGAAGAATTCTCTGCACCGAAGTCCCGGTGTGACAGGTACGGGCAGTGTGGTGCCAACAGCAGATGTAGCCCTGACAATGTTAATCTGTTTGAGTGTGACTGTTTGCCAGGGTATGTGCCTAATTCTATAAGTGATTGGAATCAGAAAAATGGTTCAGGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAAGTGTGGAGATGGAGACGGGTTTATAAAGGTGGCAAGAGTTAAATATCCGGACACATCGATAGCAGCATCGTTAAAATCAGGTATGAGTGGCAAAGAGTGTGCACAGGAGTGCCTAAGAAATTGTTCTTGCACTGCGTATTTGAGCACTGAAAATGAAGGGATTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTTTAGTGTACACAGAGCTTGGACGAGATCTCTATGTTCGTGTGAATGCAACTGTCTTAGCGGCAAATGCCGGAAGATCACAAGGTTTCTTGGAAAGGAGGGGTATGCTGGCTATTCCAATACTGTGTACTGCGCTAGCATTGGTACTAATCATTATGCGTGCATGGAAGTGGCGTAAGAAGAACAGGAACACAAGAGGCATAGACTTTGTGGAGGCAGAGGAGCTTGAGGAAACTCAGAGACACCCCGAATTGCAATTTTTCGATCTTGACACGATAATAGCAGCCACAGACCACTTCTCTCATGTCAATGAACTTGGCCGCGGTGGCTTTGGCTCTGTTTATAAGGGTCAGCTATCAAATGAACAGAAAGTTGCTGTGAAAAGATTGTCCAAAACTTCAGGACAAGGGATTGAAGAATTTAAGAATGAAGTTGCACTCATAGCAAGACTTCAACACAGGAACCTTGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATGTTAGTCCTAGAATACATGCCTAACAAAGGCTTGGACTCCTTTCTTTTTGATAAGACAAGACGGTCCTTCTTGGATTGGGAAAAGCGCTTTGAAATTATCAATGGGATTGCTCGTGGGATTCTGTATCTTCACCAAGACTCAAGATTGAGGATTATTCATAGAGACCTAAAAACTAGTAATGTTCTACTAGATGATGAGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATATTCCATGGAGACCAACTGCAAGATAAGACAAGCCGAATTGTCGGAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

719

Amino Acids

81.68

Weight (kDa)

8.17

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 130 - 240 1.4e-34 D-mannose binding lectin
S_locus_glycop PF00954 288 - 365 1.9e-12 S-locus glycoprotein domain
PAN_2 PF08276 389 - 455 5.1e-17 PAN-like domain
Pkinase PF00069 555 - 713 3.2e-33 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 557 - 710 1.4e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 483, 1187, 1707
AasI GACNNNNNNGTC 1 cut(s) 1933
AccB1I GGYRCC 1 cut(s) 1022
AccII CGCG 2 cut(s) 523, 1686
AccIII TCCGGA 1 cut(s) 1210
AciI CCGC 4 cut(s) 523, 1424, 1684, 1686
AclWI GGATC 1 cut(s) 824
AcoI YGGCCR 1 cut(s) 1681
AcsI RAATTY 6 cut(s) 331, 580, 789, 977, 1781, 2082
AcuI CTGAAG 2 cut(s) 304, 1746
AfaI GTAC 5 cut(s) 378, 1010, 1373, 1492, 1509
AfeI AGCGCT 1 cut(s) 1958
AfiI CCNNNNNNNGG 4 cut(s) 243, 740, 946, 1898
AgeI ACCGGT 1 cut(s) 342
AhlI ACTAGT 3 cut(s) 22, 466, 2045
AjnI CCWGG 6 cut(s) 227, 594, 658, 733, 832, 1078
AleI CACNNNNGTG 2 cut(s) 515, 1270
AluBI AGCT 7 cut(s) 111, 191, 339, 565, 1381, 1588, 1717
AluI AGCT 7 cut(s) 111, 191, 339, 565, 1381, 1588, 1717
Alw21I GWGCWC 4 cut(s) 444, 939, 1267, 1310
Alw26I GTCTC 4 cut(s) 1170, 1597, 2028, 2112
Alw44I GTGCAC 1 cut(s) 1263
AlwI GGATC 1 cut(s) 824
AlwNI CAGNNNCTG 1 cut(s) 920
Aor13HI TCCGGA 1 cut(s) 1210
Aor51HI AGCGCT 1 cut(s) 1958
AoxI GGCC 3 cut(s) 836, 958, 1681
ApaLI GTGCAC 1 cut(s) 1263
ApeKI GCWGC 2 cut(s) 1226, 1646
ApoI RAATTY 6 cut(s) 331, 580, 789, 977, 1781, 2082
AsiGI ACCGGT 1 cut(s) 342
Asp700I GAANNNNTTC 1 cut(s) 1285
AspLEI GCGC 2 cut(s) 1498, 1959
AspS9I GGNCC 3 cut(s) 507, 836, 1935
AsuC2I CCSGG 2 cut(s) 327, 997
AsuHPI GGTGA 4 cut(s) 97, 287, 688, 1994
AsuNHI GCTAGC 1 cut(s) 1498
AvaII GGWCC 2 cut(s) 507, 1935
BaeGI GKGCMC 1 cut(s) 1267
BaeI ACNNNNGTAYC 2 cut(s) 360, 393
BanI GGYRCC 1 cut(s) 1022
BauI CACGAG 1 cut(s) 1983
Bbv12I GWGCWC 4 cut(s) 444, 939, 1267, 1310
BbvI GCAGC 2 cut(s) 1238, 1658
BccI CCATC 5 cut(s) 266, 428, 944, 1166, 1351
BciT130I CCWGG 6 cut(s) 229, 596, 660, 735, 834, 1080
BciVI GTATCC 1 cut(s) 565
BclI TGATCA 1 cut(s) 634
BcnI CCSGG 2 cut(s) 327, 997
BcoDI GTCTC 4 cut(s) 1170, 1597, 2028, 2112
BcuI ACTAGT 3 cut(s) 22, 466, 2045
BfmI CTRYAG 2 cut(s) 642, 754
BfoI RGCGCY 1 cut(s) 1960
BfuI GTATCC 1 cut(s) 565
BglI GCCNNNNNGGC 1 cut(s) 1689
BglII AGATCT 1 cut(s) 1390
BisI GCNGC 4 cut(s) 1227, 1425, 1647, 1684
BlsI GCNGC 4 cut(s) 1228, 1426, 1648, 1685
Bme1390I CCNGG 8 cut(s) 229, 327, 596, 660, 735, 834, 997, 1080
Bme18I GGWCC 2 cut(s) 507, 1935
BmgT120I GGNCC 3 cut(s) 507, 836, 1935
BmiI GGNNCC 2 cut(s) 1024, 1826
BmrFI CCNGG 8 cut(s) 229, 327, 596, 660, 735, 834, 997, 1080
BmrI ACTGGG 2 cut(s) 699, 750
BmsI GCATC 2 cut(s) 788, 1238
BmtI GCTAGC 1 cut(s) 1502
BmuI ACTGGG 2 cut(s) 699, 750
BpuEI CTTGAG 3 cut(s) 97, 1610, 1996
BpuMI CCSGG 2 cut(s) 327, 997
Bsa29I ATCGAT 1 cut(s) 1220
BsaBI GATNNNNATC 1 cut(s) 1110
BsaI GGTCTC 2 cut(s) 2028, 2112
BsaJI CCNNGG 7 cut(s) 228, 325, 733, 833, 1079, 1684, 2113
BsaWI WCCGGW 2 cut(s) 342, 1210
BsaXI ACNNNNNCTCC 2 cut(s) 453, 483
Bsc4I CCNNNNNNNGG 4 cut(s) 243, 740, 946, 1898
Bse118I RCCGGY 1 cut(s) 342
Bse1I ACTGG 3 cut(s) 694, 745, 839
Bse8I GATNNNNATC 1 cut(s) 1110
BseAI TCCGGA 1 cut(s) 1210
BseBI CCWGG 6 cut(s) 229, 596, 660, 735, 834, 1080
BseCI ATCGAT 1 cut(s) 1220
BseDI CCNNGG 7 cut(s) 228, 325, 733, 833, 1079, 1684, 2113
BseGI GGATG 6 cut(s) 271, 604, 637, 955, 1139, 1875
BseJI GATNNNNATC 1 cut(s) 1110
BseLI CCNNNNNNNGG 4 cut(s) 243, 740, 946, 1898
BseMII CTCAG 3 cut(s) 31, 116, 1613
BseNI ACTGG 3 cut(s) 694, 745, 839
BseRI GAGGAG 1 cut(s) 1598
BseSI GKGCMC 1 cut(s) 1267
BseXI GCAGC 2 cut(s) 1238, 1658
BsgI GTGCAG 3 cut(s) 205, 543, 969
Bsh1236I CGCG 2 cut(s) 523, 1686
Bsh1285I CGRYCG 1 cut(s) 527
BshFI GGCC 3 cut(s) 838, 960, 1683
BshNI GGYRCC 1 cut(s) 1022
BshTI ACCGGT 1 cut(s) 342
BshVI ATCGAT 1 cut(s) 1220
BsiEI CGRYCG 1 cut(s) 527
BsiHKAI GWGCWC 4 cut(s) 444, 939, 1267, 1310
BsiSI CCGG 5 cut(s) 327, 343, 997, 1211, 1434
BslFI GGGAC 3 cut(s) 245, 758, 979
BslI CCNNNNNNNGG 4 cut(s) 243, 740, 946, 1898
BsmAI GTCTC 4 cut(s) 1170, 1597, 2028, 2112
BsmBI CGTCTC 1 cut(s) 1170
BsmFI GGGAC 3 cut(s) 245, 758, 979
BsmI GAATGC 1 cut(s) 1414
BsnI GGCC 3 cut(s) 838, 960, 1683
Bso31I GGTCTC 2 cut(s) 2028, 2112
Bsp1286I GDGCHC 4 cut(s) 444, 939, 1267, 1310
Bsp13I TCCGGA 1 cut(s) 1210
Bsp1407I TGTACA 1 cut(s) 1371
Bsp143I GATC 7 cut(s) 75, 634, 769, 829, 1390, 1439, 1627
Bsp19I CCATGG 1 cut(s) 2113
BspACI CCGC 4 cut(s) 523, 1424, 1684, 1686
BspANI GGCC 3 cut(s) 838, 960, 1683
BspCNI CTCAG 3 cut(s) 30, 117, 1612
BspDI ATCGAT 1 cut(s) 1220
BspEI TCCGGA 1 cut(s) 1210
BspFNI CGCG 2 cut(s) 523, 1686
BspHI TCATGA 1 cut(s) 882
BspLI GGNNCC 2 cut(s) 1024, 1826
BspOI GCTAGC 1 cut(s) 1502
BspPI GGATC 1 cut(s) 824
BspT107I GGYRCC 1 cut(s) 1022
BspTNI GGTCTC 2 cut(s) 2028, 2112
BsrFI RCCGGY 1 cut(s) 342
BsrGI TGTACA 1 cut(s) 1371
BsrI ACTGG 3 cut(s) 694, 745, 839
BssAI RCCGGY 1 cut(s) 342
BssECI CCNNGG 7 cut(s) 228, 325, 733, 833, 1079, 1684, 2113
BssMI GATC 7 cut(s) 75, 634, 769, 829, 1390, 1439, 1627
BssSI CACGAG 1 cut(s) 1983
BssT1I CCWWGG 1 cut(s) 2113
Bst2BI CACGAG 1 cut(s) 1983
Bst2UI CCWGG 6 cut(s) 229, 596, 660, 735, 834, 1080
Bst4CI ACNGT 6 cut(s) 391, 916, 1073, 1417, 1488, 1935
Bst6I CTCTTC 1 cut(s) 212
BstAUI TGTACA 1 cut(s) 1371
BstC8I GCNNGC 3 cut(s) 1473, 1500, 1524
BstDEI CTNAG 7 cut(s) 17, 125, 761, 931, 1277, 1420, 1599
BstDSI CCRYGG 2 cut(s) 1684, 2113
BstENI CCTNNNNNAGG 1 cut(s) 1896
BstF5I GGATG 6 cut(s) 271, 604, 637, 955, 1139, 1875
BstFNI CGCG 2 cut(s) 523, 1686
BstH2I RGCGCY 1 cut(s) 1960
BstHHI GCGC 2 cut(s) 1498, 1959
BstKTI GATC 7 cut(s) 78, 637, 772, 832, 1393, 1442, 1630
BstMAI GTCTC 4 cut(s) 1170, 1597, 2028, 2112
BstMBI GATC 7 cut(s) 75, 634, 769, 829, 1390, 1439, 1627
BstMCI CGRYCG 1 cut(s) 527
BstMWI GCNNNNNNNGC 4 cut(s) 1038, 1430, 1689, 1805
BstNI CCWGG 6 cut(s) 229, 596, 660, 735, 834, 1080
BstNSI RCATGY 1 cut(s) 1891
BstSCI CCNGG 8 cut(s) 227, 325, 594, 658, 733, 832, 995, 1078
BstSFI CTRYAG 2 cut(s) 642, 754
BstSLI GKGCMC 1 cut(s) 1267
BstUI CGCG 2 cut(s) 523, 1686
BstV1I GCAGC 2 cut(s) 1238, 1658
BstX2I RGATCY 1 cut(s) 1390
BstYI RGATCY 1 cut(s) 1390
Bsu15I ATCGAT 1 cut(s) 1220
BsuI GTATCC 1 cut(s) 565
BsuRI GGCC 3 cut(s) 838, 960, 1683
BsuTUI ATCGAT 1 cut(s) 1220
BtgI CCRYGG 2 cut(s) 1684, 2113
BtsCI GGATG 6 cut(s) 271, 604, 637, 955, 1139, 1875
BtsI GCAGTG 2 cut(s) 1022, 1293
BtsIMutI CAGTG 6 cut(s) 396, 846, 921, 1022, 1293, 1308
Cac8I GCNNGC 3 cut(s) 1473, 1500, 1524
CaiI CAGNNNCTG 1 cut(s) 920
CciI TCATGA 1 cut(s) 882
CfoI GCGC 2 cut(s) 1498, 1959
Cfr10I RCCGGY 1 cut(s) 342
Cfr13I GGNCC 3 cut(s) 507, 836, 1935
Cfr42I CCGCGG 1 cut(s) 1687
ClaI ATCGAT 1 cut(s) 1220
Csp6I GTAC 5 cut(s) 377, 1009, 1372, 1491, 1508
CspAI ACCGGT 1 cut(s) 342
CviAII CATG 9 cut(s) 238, 487, 883, 1341, 1527, 1667, 1888, 2098, 2114
CviQI GTAC 5 cut(s) 377, 1009, 1372, 1491, 1508
DdeI CTNAG 7 cut(s) 17, 125, 761, 931, 1277, 1420, 1599
DpnI GATC 7 cut(s) 77, 636, 771, 831, 1392, 1441, 1629
DpnII GATC 7 cut(s) 75, 634, 769, 829, 1390, 1439, 1627
DrdI GACNNNNNNGTC 1 cut(s) 1933
DseDI GACNNNNNNGTC 1 cut(s) 1933
EaeI YGGCCR 1 cut(s) 1681
Eam1104I CTCTTC 1 cut(s) 212
EarI CTCTTC 1 cut(s) 212
Eco130I CCWWGG 1 cut(s) 2113
Eco31I GGTCTC 2 cut(s) 2028, 2112
Eco47I GGWCC 2 cut(s) 507, 1935
Eco47III AGCGCT 1 cut(s) 1958
Eco57I CTGAAG 2 cut(s) 304, 1746
EcoNI CCTNNNNNAGG 1 cut(s) 1896
EcoRI GAATTC 1 cut(s) 977
EcoRII CCWGG 6 cut(s) 227, 594, 658, 733, 832, 1078
EcoT14I CCWWGG 1 cut(s) 2113
EcoT22I ATGCAT 1 cut(s) 781
ErhI CCWWGG 1 cut(s) 2113
Esp3I CGTCTC 1 cut(s) 1170
FaeI CATG 9 cut(s) 241, 490, 886, 1344, 1530, 1670, 1891, 2101, 2117
FalI AAGNNNNNCTT 2 cut(s) 180, 212
FaqI GGGAC 3 cut(s) 245, 758, 979
FatI CATG 9 cut(s) 237, 486, 882, 1340, 1526, 1666, 1887, 2097, 2113
FbaI TGATCA 1 cut(s) 634
Fnu4HI GCNGC 4 cut(s) 1227, 1425, 1647, 1684
FokI GGATG 6 cut(s) 278, 611, 624, 962, 1146, 1882
Fsp4HI GCNGC 4 cut(s) 1227, 1425, 1647, 1684
GlaI GCGC 2 cut(s) 1497, 1958
GluI GCNGC 4 cut(s) 1227, 1425, 1647, 1684
HaeII RGCGCY 1 cut(s) 1960
HaeIII GGCC 3 cut(s) 838, 960, 1683
HapII CCGG 5 cut(s) 327, 343, 997, 1211, 1434
HhaI GCGC 2 cut(s) 1498, 1959
Hin1II CATG 9 cut(s) 241, 490, 886, 1344, 1530, 1670, 1891, 2101, 2117
Hin6I GCGC 2 cut(s) 1496, 1957
HinP1I GCGC 2 cut(s) 1496, 1957
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HinfI GANTC 7 cut(s) 90, 354, 923, 1111, 1907, 1990, 2009
HpaI GTTAAC 1 cut(s) 541
HpaII CCGG 5 cut(s) 327, 343, 997, 1211, 1434
HphI GGTGA 4 cut(s) 97, 287, 688, 1994
Hpy166II GTNNAC 6 cut(s) 510, 541, 1265, 1372, 1374, 1491
Hpy188I TCNGA 6 cut(s) 20, 769, 1116, 1602, 2089, 2153
Hpy188III TCNNGA 6 cut(s) 863, 883, 1211, 1631, 1764, 2013
Hpy8I GTNNAC 6 cut(s) 510, 541, 1265, 1372, 1374, 1491
HpyAV CCTTC 5 cut(s) 256, 964, 971, 1313, 1948
HpyCH4III ACNGT 6 cut(s) 391, 916, 1073, 1417, 1488, 1935
HpyF10VI GCNNNNNNNGC 4 cut(s) 1038, 1430, 1689, 1805
HpyF3I CTNAG 7 cut(s) 17, 125, 761, 931, 1277, 1420, 1599
Hsp92II CATG 9 cut(s) 241, 490, 886, 1344, 1530, 1670, 1891, 2101, 2117
HspAI GCGC 2 cut(s) 1496, 1957
Kpn2I TCCGGA 1 cut(s) 1210
Ksp22I TGATCA 1 cut(s) 634
KspAI GTTAAC 1 cut(s) 541
KspI CCGCGG 1 cut(s) 1687
Kzo9I GATC 7 cut(s) 75, 634, 769, 829, 1390, 1439, 1627
LmnI GCTCC 1 cut(s) 1585
Lsp1109I GCAGC 2 cut(s) 1238, 1658
LweI GCATC 2 cut(s) 788, 1238
MaeIII GTNAC 5 cut(s) 275, 720, 916, 1001, 1067
MalI GATC 7 cut(s) 77, 636, 771, 831, 1392, 1441, 1629
MboI GATC 7 cut(s) 75, 634, 769, 829, 1390, 1439, 1627
MfeI CAATTG 1 cut(s) 257
MflI RGATCY 1 cut(s) 1390
MhlI GDGCHC 4 cut(s) 444, 939, 1267, 1310
MlyI GAGTC 3 cut(s) 84, 1901, 2003
MmeI TCCRAC 3 cut(s) 177, 331, 2131
MnlI CCTC 8 cut(s) 236, 442, 1455, 1553, 1570, 1576, 1585, 2013
Mph1103I ATGCAT 1 cut(s) 781
MroI TCCGGA 1 cut(s) 1210
MroXI GAANNNNTTC 1 cut(s) 1285
MseI TTAA 7 cut(s) 540, 653, 707, 1053, 1203, 1235, 1785
MslI CAYNNNNRTG 5 cut(s) 515, 1270, 1367, 1531, 1671
MspA1I CMGCKG 1 cut(s) 1686
MspI CCGG 5 cut(s) 327, 343, 997, 1211, 1434
MspR9I CCNGG 8 cut(s) 229, 327, 596, 660, 735, 834, 997, 1080
MunI CAATTG 1 cut(s) 257
Mva1269I GAATGC 1 cut(s) 1414
MvaI CCWGG 6 cut(s) 229, 596, 660, 735, 834, 1080
MvnI CGCG 2 cut(s) 523, 1686
MwoI GCNNNNNNNGC 4 cut(s) 1038, 1430, 1689, 1805
NciI CCSGG 2 cut(s) 327, 997
NcoI CCATGG 1 cut(s) 2113
NdeII GATC 7 cut(s) 75, 634, 769, 829, 1390, 1439, 1627
NheI GCTAGC 1 cut(s) 1498
NlaIII CATG 9 cut(s) 241, 490, 886, 1344, 1530, 1670, 1891, 2101, 2117
NlaIV GGNNCC 2 cut(s) 1024, 1826
NmeAIII GCCGAG 1 cut(s) 196
NmuCI GTSAC 5 cut(s) 275, 720, 916, 1001, 1067
NsiI ATGCAT 1 cut(s) 781
NspI RCATGY 1 cut(s) 1891
OliI CACNNNNGTG 2 cut(s) 515, 1270
PagI TCATGA 1 cut(s) 882
PctI GAATGC 1 cut(s) 1414
PdmI GAANNNNTTC 1 cut(s) 1285
PfeI GAWTC 4 cut(s) 354, 923, 1111, 1990
PfoI TCCNGGA 1 cut(s) 594
PinAI ACCGGT 1 cut(s) 342
PkrI GCNGC 4 cut(s) 1228, 1426, 1648, 1685
PleI GAGTC 3 cut(s) 84, 1901, 2003
PpsI GAGTC 3 cut(s) 84, 1901, 2003
PsiI TTATAA 3 cut(s) 483, 1187, 1707
Psp6I CCWGG 6 cut(s) 227, 594, 658, 733, 832, 1078
PspGI CCWGG 6 cut(s) 227, 594, 658, 733, 832, 1078
PspN4I GGNNCC 2 cut(s) 1024, 1826
PspPI GGNCC 3 cut(s) 507, 836, 1935
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 1 cut(s) 1390
RsaI GTAC 5 cut(s) 378, 1010, 1373, 1492, 1509
RsaNI GTAC 5 cut(s) 377, 1009, 1372, 1491, 1508
RseI CAYNNNNRTG 5 cut(s) 515, 1270, 1367, 1531, 1671
SacII CCGCGG 1 cut(s) 1687
SaqAI TTAA 7 cut(s) 540, 653, 707, 1053, 1203, 1235, 1785
SatI GCNGC 4 cut(s) 1227, 1425, 1647, 1684
Sau3AI GATC 7 cut(s) 75, 634, 769, 829, 1390, 1439, 1627
Sau96I GGNCC 3 cut(s) 507, 836, 1935
SchI GAGTC 3 cut(s) 84, 1901, 2003
ScrFI CCNGG 8 cut(s) 229, 327, 596, 660, 735, 834, 997, 1080
SduI GDGCHC 4 cut(s) 444, 939, 1267, 1310
SfaNI GCATC 2 cut(s) 788, 1238
SfcI CTRYAG 2 cut(s) 642, 754
Sfr303I CCGCGG 1 cut(s) 1687
SgrBI CCGCGG 1 cut(s) 1687
SinI GGWCC 2 cut(s) 507, 1935
SmiMI CAYNNNNRTG 5 cut(s) 515, 1270, 1367, 1531, 1671
SmlI CTYRAG 3 cut(s) 112, 1589, 2011
SmoI CTYRAG 3 cut(s) 112, 1589, 2011
SpeI ACTAGT 3 cut(s) 22, 466, 2045
SsiI CCGC 4 cut(s) 523, 1424, 1684, 1686
SspI AATATT 1 cut(s) 2109
StyD4I CCNGG 8 cut(s) 227, 325, 594, 658, 733, 832, 995, 1078
StyI CCWWGG 1 cut(s) 2113
TaaI ACNGT 6 cut(s) 391, 916, 1073, 1417, 1488, 1935
TaqI TCGA 3 cut(s) 1147, 1220, 1626
TatI WGTACW 2 cut(s) 1371, 1490
TauI GCSGC 2 cut(s) 1427, 1686
TfiI GAWTC 4 cut(s) 354, 923, 1111, 1990
Tru1I TTAA 7 cut(s) 540, 653, 707, 1053, 1203, 1235, 1785
Tru9I TTAA 7 cut(s) 540, 653, 707, 1053, 1203, 1235, 1785
TscAI CASTG 6 cut(s) 396, 846, 921, 1022, 1300, 1315
TseFI GTSAC 5 cut(s) 275, 720, 916, 1001, 1067
TseI GCWGC 2 cut(s) 1226, 1646
Tsp45I GTSAC 5 cut(s) 275, 720, 916, 1001, 1067
TspRI CASTG 6 cut(s) 396, 846, 921, 1022, 1300, 1315
VneI GTGCAC 1 cut(s) 1263
VpaK11BI GGWCC 2 cut(s) 507, 1935
XagI CCTNNNNNAGG 1 cut(s) 1896
XapI RAATTY 6 cut(s) 331, 580, 789, 977, 1781, 2082
XceI RCATGY 1 cut(s) 1891
XmnI GAANNNNTTC 1 cut(s) 1285
Zsp2I ATGCAT 1 cut(s) 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.