FvH4_3g03370

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000112
Physical Location & Seq
Reverse (-)
1829057 .. 1832851
3795 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g03370.t1

Sequence Viewer

Length: 2439 bp
ATGCTACTGAAATCTCTGCTTCTCCTCCTCCTTTTCCAATTGTGCACTTCCAGGGACTACATAACATGGGACTGGAACGAACATCAACTAAAGGATGGGGACCTTTTAGTGTCCAAAGGCAACAACTTTGAATTGGGTTTCTTCAGCCCCAGAAACTCTAGCCACCGCTATGTGGGAATTTGGTATTCAAAGTCTAAGATATCTGAACAAACAGTGGTGTGGGTGGCAAACAGAAACGACCCCATCAATGATACCTCTGGTGTTCTCACAATGAACAGGTATGGAAATCTAGTCATTTATGCTCACAATATGGCGAACATTCCTATATGGTCTACAAATATGTCTGAGTCAGCAGTCATAAACACATATCCCGTATCTGCACAACTTTTAGATACAGGAAACTTAGTTCTGACCCAGGATGGTGAAAGTGAAAGATATATTAAATGGCAGAGTTTTGATTACCCTACAGATACTCTGATCCCTGGTATGAAACTTGGGGTGAATTGGAAAACTGGTTTAGAGTGGTTTTTATCATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATAGCTATAGGCTTCAGTGGAATCAGACTGCTGTGCCACAATTTTTTCTGTTTAAGGGTTTAACTAGGCTTTGGAGAAGTGATCCTGGGCCGTGGCGCAGTTTTGTTACTAATAATGATGAGATGTATTTCCTTCAATATAGTGATAACACCTCTGAAAACCTTAGAATGGTGGTGAATGATTCCGGGTTATTGCAGCATTTGACATGGAATGATGATGACCGTCAATGGAAGGAACTATGGGCTGCACCGAGATACCGGTGTGACTTGTATGGACAGTGTGGTGCCTATAGTAAATGTAACCCTGACAATATTAATGTGTTTGAGTGTGAGTGTTTACCAGGGTATGAGCCTAAATATTCAAATAACTGGAATCAGAATGATGGGTCAGATGGGTGTGTGAGTAAGCGAGTTGGAGTGTCCAAATGTGGAATTGGAGATGGGTTTGTGCTGGTTGCAAGGGTGAAGGCTCCAGACACATCAAATGTAGTAGCGCGACTGAATACTAGTACAAGTGCCAAAGAGTGCAAGCAGGAGTGTTTGAGAAATTGTTCTTGCACAGCATATATGAGCATTGATAATAAAGAGCACATTGATTGCTTGACATGGTATGGTGAGATGCTAGATACAGTTGGGCACACAGATGCAGGACAAGATCTATTTGTTCGTGTGGATGAAATCGGGTTAGCCATGATTTCCAGAAAATCAAAAGGTTTTCTGAAAAGGAGGGGCATGCTTGCTATTCTGATAGTGTCTGGTCTGCTGGTGCTGGTACTAATCGCTGTGTTTGGCTGTTGGTGGCTTAGGAAAATGAGGAAGACAAAAGACTTTGCAGTGGCGGATGAACTAGGAAAAGCTAGGAAACACCCTGATCTGCAATTTTTCTCTTTCAGCACAATAATTGTAGCCACGTCTAACTTCCATTTTGAAAATAAGCTTGGGGAAGGAGGTTTTGGCTCGGTTTATAAGGGTAAGTTACCAAGTGGCCAGGAGATTGCTGTAAAAAGATTGTCCAGAACTTCAGGGCAAGGGATTGAAGAATTCAAAAATGAAGTTGTGCTTATAGAAAGACTCCAACACAGGAACCTTGTGAAACTCTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATGTTAGTGCTAGAATACATGCCTAACAAAAGCTTGGACTCCTTTCTTTTTGATCACACAAAAAGCTCCTTGGATTGGGAAAAGCGCTTTGAAATCATCAATGGCATTGCTCGCGGTATTCTTTATCTTCACCAGGACTCAAGAGTTAGGATTATACATAGGGATCTGAAAACCAGCAATGTTCTTCTAGATGCTGAGATGAATCCAAAAATTTCAGACTTTGGGATGGCAAGAATTTTTTATGGGGACCAATTGCAGGAAAAGACAGCCAGAATTGCTGGAACATATGGATATATGGCACCAGAATATGCAGTTTTTGGAAGATATTCAGCAAAATCGGATGTCTTTAGCTTTGGGATCATAATATTGGAGATCGTAAGTGGAAAAAAGAACTCTGGTTCTTATCAGGAGGAGCATTCCATGAGCTTGATAGGAAATGTTTGGCAGATGTGGGGTGAAGACAGAGCCTTGGAAATACTGGATTCATCACTACAGTTATATAAACCAGATGAAGCCTTGAGATGCATTCAAGTTGGGCTCTTATGTGTGCAAGAAGATCCAAAGGACCGACCTACCATGTCAGCTGCTGTTTTCATGTTGAGTGGTGAAGCATCTCTTTCAACTCCTAAGCAGCCTGCATTTGTTTTTAGGAAAAGTGCAGGTAGTAATGCTTCATCCCTTACGGATGAATTATGTTCAATTAACGACTTAACAGTGACCATTGTGGAAGCTCGAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

813

Amino Acids

92.08

Weight (kDa)

6.19

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 71 - 181 4.6e-35 D-mannose binding lectin
S_locus_glycop PF00954 224 - 309 6.1e-19 S-locus glycoprotein domain
PAN_2 PF08276 333 - 400 2.3e-16 PAN-like domain
Pkinase PF00069 497 - 762 3.6e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 498 - 762 1.2e-52 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 769 - 811 3e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1536
Acc36I ACCTGC 1 cut(s) 2348
AccB1I GGYRCC 2 cut(s) 854, 1996
AccI GTMKAC 1 cut(s) 332
AccII CGCG 2 cut(s) 1066, 1812
AciI CCGC 3 cut(s) 166, 1409, 1812
AclWI GGATC 5 cut(s) 472, 647, 1869, 2063, 2249
AcoI YGGCCR 1 cut(s) 1555
AcsI RAATTY 4 cut(s) 177, 1610, 1908, 1932
AcuI CTGAAG 3 cut(s) 127, 569, 1575
AfaI GTAC 2 cut(s) 1081, 1344
AfeI AGCGCT 1 cut(s) 1784
AfiI CCNNNNNNNGG 5 cut(s) 172, 563, 739, 1773, 1954
AgeI ACCGGT 1 cut(s) 828
AhlI ACTAGT 1 cut(s) 1076
AjiI CACGTC 1 cut(s) 1482
AjnI CCWGG 8 cut(s) 50, 414, 481, 556, 655, 910, 1557, 1830
AjuI GAANNNNNNNTTGG 4 cut(s) 1491, 1506, 1523, 1538
AloI GAACNNNNNNTCC 2 cut(s) 390, 422
AluBI AGCT 9 cut(s) 576, 1427, 1507, 1731, 1764, 2049, 2124, 2282, 2429
AluI AGCT 9 cut(s) 576, 1427, 1507, 1731, 1764, 2049, 2124, 2282, 2429
Alw21I GWGCWC 2 cut(s) 47, 1161
Alw44I GTGCAC 1 cut(s) 43
AlwI GGATC 5 cut(s) 472, 647, 1869, 2063, 2249
AlwNI CAGNNNCTG 1 cut(s) 2285
Ama87I CYCGRG 1 cut(s) 2430
Aor51HI AGCGCT 1 cut(s) 1784
AoxI GGCC 2 cut(s) 659, 1555
ApaLI GTGCAC 1 cut(s) 43
ApeKI GCWGC 4 cut(s) 766, 815, 2282, 2329
ApoI RAATTY 4 cut(s) 177, 1610, 1908, 1932
AseI ATTAAT 1 cut(s) 885
AsiGI ACCGGT 1 cut(s) 828
Asp700I GAANNNNTTC 2 cut(s) 1120, 2023
AspLEI GCGC 3 cut(s) 669, 1066, 1785
AspS9I GGNCC 4 cut(s) 100, 659, 1945, 2263
AsuC2I CCSGG 1 cut(s) 757
AsuHPI GGTGA 8 cut(s) 434, 511, 757, 1045, 1196, 1820, 2165, 2315
AvaI CYCGRG 1 cut(s) 2430
AvaII GGWCC 3 cut(s) 100, 1945, 2263
BaeGI GKGCMC 2 cut(s) 47, 1209
BaeI ACNNNNGTAYC 2 cut(s) 817, 850
BalI TGGCCA 1 cut(s) 1557
BanI GGYRCC 2 cut(s) 854, 1996
BanII GRGCYC 1 cut(s) 2238
BarI GAAGNNNNNNTAC 2 cut(s) 2353, 2385
BbsI GAAGAC 2 cut(s) 1394, 2163
Bbv12I GWGCWC 2 cut(s) 47, 1161
BbvI GCAGC 4 cut(s) 778, 802, 2269, 2341
BccI CCATC 7 cut(s) 89, 251, 413, 947, 956, 1004, 1918
BceAI ACGGC 1 cut(s) 646
BciT130I CCWGG 8 cut(s) 52, 416, 483, 558, 657, 912, 1559, 1832
BclI TGATCA 1 cut(s) 1750
BcnI CCSGG 1 cut(s) 757
BcuI ACTAGT 1 cut(s) 1076
BfaI CTAG 9 cut(s) 159, 290, 636, 1077, 1193, 1418, 1428, 1709, 1886
BfmI CTRYAG 5 cut(s) 465, 571, 577, 859, 2189
BfoI RGCGCY 1 cut(s) 1786
BfuAI ACCTGC 1 cut(s) 2348
BglII AGATCT 1 cut(s) 1225
BisI GCNGC 4 cut(s) 767, 816, 2283, 2330
BlsI GCNGC 4 cut(s) 768, 817, 2284, 2331
Bme1390I CCNGG 9 cut(s) 52, 416, 483, 558, 657, 757, 912, 1559, 1832
Bme18I GGWCC 3 cut(s) 100, 1945, 2263
BmeT110I CYCGRG 1 cut(s) 2430
BmgBI CACGTC 1 cut(s) 1482
BmgT120I GGNCC 4 cut(s) 100, 659, 1945, 2263
BmiI GGNNCC 6 cut(s) 101, 856, 1041, 1655, 1946, 1998
BmrFI CCNGG 9 cut(s) 52, 416, 483, 558, 657, 757, 912, 1559, 1832
BmrI ACTGGG 1 cut(s) 573
BmsI GCATC 5 cut(s) 1179, 1204, 1879, 2210, 2318
BmuI ACTGGG 1 cut(s) 573
BpiI GAAGAC 2 cut(s) 1394, 2163
BpmI CTGGAG 1 cut(s) 1026
Bpu10I CCTNAGC 2 cut(s) 1373, 2325
BpuEI CTTGAG 2 cut(s) 1822, 2236
BpuMI CCSGG 1 cut(s) 757
BsaJI CCNNGG 9 cut(s) 51, 414, 481, 556, 656, 662, 911, 1767, 2166
BsaWI WCCGGW 1 cut(s) 828
Bsc4I CCNNNNNNNGG 5 cut(s) 172, 563, 739, 1773, 1954
Bse118I RCCGGY 1 cut(s) 828
Bse1I ACTGG 5 cut(s) 77, 517, 568, 944, 2181
Bse3DI GCAATG 2 cut(s) 1803, 1882
BseBI CCWGG 8 cut(s) 52, 416, 483, 558, 657, 912, 1559, 1832
BseDI CCNNGG 9 cut(s) 51, 414, 481, 556, 656, 662, 911, 1767, 2166
BseGI GGATG 9 cut(s) 100, 424, 1249, 1417, 1704, 1929, 2044, 2372, 2389
BseLI CCNNNNNNNGG 5 cut(s) 172, 563, 739, 1773, 1954
BseMI GCAATG 2 cut(s) 1803, 1882
BseMII CTCAG 2 cut(s) 336, 1884
BseNI ACTGG 5 cut(s) 77, 517, 568, 944, 2181
BseRI GAGGAG 3 cut(s) 14, 17, 2123
BseSI GKGCMC 2 cut(s) 47, 1209
BseXI GCAGC 4 cut(s) 778, 802, 2269, 2341
BsgI GTGCAG 3 cut(s) 363, 801, 2376
Bsh1236I CGCG 2 cut(s) 1066, 1812
BshFI GGCC 2 cut(s) 661, 1557
BshNI GGYRCC 2 cut(s) 854, 1996
BshTI ACCGGT 1 cut(s) 828
BsiHKAI GWGCWC 2 cut(s) 47, 1161
BsiHKCI CYCGRG 1 cut(s) 2430
BsiSI CCGG 2 cut(s) 756, 829
BslFI GGGAC 5 cut(s) 68, 83, 113, 581, 1958
BslI CCNNNNNNNGG 5 cut(s) 172, 563, 739, 1773, 1954
BsmFI GGGAC 5 cut(s) 68, 83, 113, 581, 1958
BsmI GAATGC 2 cut(s) 2113, 2223
BsnI GGCC 2 cut(s) 661, 1557
BsoBI CYCGRG 1 cut(s) 2430
Bsp1286I GDGCHC 4 cut(s) 47, 1161, 1209, 2238
Bsp143I GATC 9 cut(s) 477, 652, 1225, 1441, 1750, 1861, 2055, 2070, 2254
BspACI CCGC 3 cut(s) 166, 1409, 1812
BspANI GGCC 2 cut(s) 661, 1557
BspCNI CTCAG 2 cut(s) 337, 1885
BspFNI CGCG 2 cut(s) 1066, 1812
BspLI GGNNCC 6 cut(s) 101, 856, 1041, 1655, 1946, 1998
BspMI ACCTGC 1 cut(s) 2348
BspPI GGATC 5 cut(s) 472, 647, 1869, 2063, 2249
BspT107I GGYRCC 2 cut(s) 854, 1996
BsrDI GCAATG 2 cut(s) 1803, 1882
BsrFI RCCGGY 1 cut(s) 828
BsrI ACTGG 5 cut(s) 77, 517, 568, 944, 2181
BssAI RCCGGY 1 cut(s) 828
BssECI CCNNGG 9 cut(s) 51, 414, 481, 556, 656, 662, 911, 1767, 2166
BssMI GATC 9 cut(s) 477, 652, 1225, 1441, 1750, 1861, 2055, 2070, 2254
BssT1I CCWWGG 2 cut(s) 1767, 2166
Bst2UI CCWGG 8 cut(s) 52, 416, 483, 558, 657, 912, 1559, 1832
Bst4CI ACNGT 6 cut(s) 214, 794, 849, 1201, 2193, 2413
BstC8I GCNNGC 5 cut(s) 1100, 1304, 1308, 1810, 2334
BstDEI CTNAG 8 cut(s) 195, 345, 403, 734, 1373, 1669, 1893, 2325
BstDSI CCRYGG 1 cut(s) 662
BstF5I GGATG 9 cut(s) 100, 424, 1249, 1417, 1704, 1929, 2044, 2372, 2389
BstFNI CGCG 2 cut(s) 1066, 1812
BstH2I RGCGCY 1 cut(s) 1786
BstHHI GCGC 3 cut(s) 669, 1066, 1785
BstKTI GATC 9 cut(s) 480, 655, 1228, 1444, 1753, 1864, 2058, 2073, 2257
BstMBI GATC 9 cut(s) 477, 652, 1225, 1441, 1750, 1861, 2055, 2070, 2254
BstMWI GCNNNNNNNGC 2 cut(s) 1809, 1973
BstNI CCWGG 8 cut(s) 52, 416, 483, 558, 657, 912, 1559, 1832
BstNSI RCATGY 2 cut(s) 1306, 1720
BstSCI CCNGG 9 cut(s) 50, 414, 481, 556, 655, 755, 910, 1557, 1830
BstSFI CTRYAG 5 cut(s) 465, 571, 577, 859, 2189
BstSLI GKGCMC 2 cut(s) 47, 1209
BstUI CGCG 2 cut(s) 1066, 1812
BstV1I GCAGC 4 cut(s) 778, 802, 2269, 2341
BstV2I GAAGAC 2 cut(s) 1394, 2163
BstX2I RGATCY 3 cut(s) 1225, 1861, 2254
BstYI RGATCY 3 cut(s) 1225, 1861, 2254
BsuRI GGCC 2 cut(s) 661, 1557
BtgI CCRYGG 1 cut(s) 662
BtrI CACGTC 1 cut(s) 1482
BtsCI GGATG 9 cut(s) 100, 424, 1249, 1417, 1704, 1929, 2044, 2372, 2389
BtsI GCAGTG 1 cut(s) 1410
BtsIMutI CAGTG 5 cut(s) 219, 593, 854, 1410, 2418
BveI ACCTGC 1 cut(s) 2348
Cac8I GCNNGC 5 cut(s) 1100, 1304, 1308, 1810, 2334
CaiI CAGNNNCTG 1 cut(s) 2285
CfoI GCGC 3 cut(s) 669, 1066, 1785
Cfr10I RCCGGY 1 cut(s) 828
Cfr13I GGNCC 4 cut(s) 100, 659, 1945, 2263
Csp6I GTAC 2 cut(s) 1080, 1343
CspAI ACCGGT 1 cut(s) 828
CviAII CATG 9 cut(s) 66, 777, 1176, 1261, 1303, 1717, 2119, 2275, 2293
CviQI GTAC 2 cut(s) 1080, 1343
DdeI CTNAG 8 cut(s) 195, 345, 403, 734, 1373, 1669, 1893, 2325
DpnI GATC 9 cut(s) 479, 654, 1227, 1443, 1752, 1863, 2057, 2072, 2256
DpnII GATC 9 cut(s) 477, 652, 1225, 1441, 1750, 1861, 2055, 2070, 2254
EaeI YGGCCR 1 cut(s) 1555
EciI GGCGGA 1 cut(s) 1424
Eco130I CCWWGG 2 cut(s) 1767, 2166
Eco24I GRGCYC 1 cut(s) 2238
Eco32I GATATC 1 cut(s) 201
Eco47I GGWCC 3 cut(s) 100, 1945, 2263
Eco47III AGCGCT 1 cut(s) 1784
Eco57I CTGAAG 3 cut(s) 127, 569, 1575
Eco88I CYCGRG 1 cut(s) 2430
EcoO109I RGGNCCY 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 1610
EcoRII CCWGG 8 cut(s) 50, 414, 481, 556, 655, 910, 1557, 1830
EcoRV GATATC 1 cut(s) 201
EcoT14I CCWWGG 2 cut(s) 1767, 2166
EcoT22I ATGCAT 1 cut(s) 2225
EcoT38I GRGCYC 1 cut(s) 2238
ErhI CCWWGG 2 cut(s) 1767, 2166
FaeI CATG 9 cut(s) 69, 780, 1179, 1264, 1306, 1720, 2122, 2278, 2296
FalI AAGNNNNNCTT 4 cut(s) 1614, 1646, 2298, 2330
FaqI GGGAC 5 cut(s) 68, 83, 113, 581, 1958
FatI CATG 9 cut(s) 65, 776, 1175, 1260, 1302, 1716, 2118, 2274, 2292
FauNDI CATATG 1 cut(s) 1984
FbaI TGATCA 1 cut(s) 1750
FblI GTMKAC 1 cut(s) 332
Fnu4HI GCNGC 4 cut(s) 767, 816, 2283, 2330
FokI GGATG 9 cut(s) 107, 431, 1256, 1424, 1711, 1936, 2051, 2359, 2396
FriOI GRGCYC 1 cut(s) 2238
Fsp4HI GCNGC 4 cut(s) 767, 816, 2283, 2330
FspBI CTAG 9 cut(s) 159, 290, 636, 1077, 1193, 1418, 1428, 1709, 1886
GlaI GCGC 3 cut(s) 668, 1065, 1784
GluI GCNGC 4 cut(s) 767, 816, 2283, 2330
GsuI CTGGAG 1 cut(s) 1026
HaeII RGCGCY 1 cut(s) 1786
HaeIII GGCC 2 cut(s) 661, 1557
HapII CCGG 2 cut(s) 756, 829
HhaI GCGC 3 cut(s) 669, 1066, 1785
Hin1II CATG 9 cut(s) 69, 780, 1179, 1264, 1306, 1720, 2122, 2278, 2296
Hin6I GCGC 3 cut(s) 667, 1064, 1783
HinP1I GCGC 3 cut(s) 667, 1064, 1783
HindIII AAGCTT 2 cut(s) 1505, 1729
HinfI GANTC 9 cut(s) 347, 592, 752, 943, 1641, 1736, 1835, 1900, 2180
HpaII CCGG 2 cut(s) 756, 829
HphI GGTGA 8 cut(s) 434, 511, 757, 1045, 1196, 1820, 2165, 2315
Hpy166II GTNNAC 3 cut(s) 45, 333, 908
Hpy188III TCNNGA 7 cut(s) 1043, 1269, 1584, 1839, 1886, 2105, 2432
Hpy8I GTNNAC 3 cut(s) 45, 333, 908
HpyAV CCTTC 4 cut(s) 713, 796, 1030, 1508
HpyCH4III ACNGT 6 cut(s) 214, 794, 849, 1201, 2193, 2413
HpyCH4IV ACGT 1 cut(s) 1481
HpyF10VI GCNNNNNNNGC 2 cut(s) 1809, 1973
HpyF3I CTNAG 8 cut(s) 195, 345, 403, 734, 1373, 1669, 1893, 2325
HpySE526I ACGT 1 cut(s) 1481
Hsp92II CATG 9 cut(s) 69, 780, 1179, 1264, 1306, 1720, 2122, 2278, 2296
HspAI GCGC 3 cut(s) 667, 1064, 1783
Ksp22I TGATCA 1 cut(s) 1750
Kzo9I GATC 9 cut(s) 477, 652, 1225, 1441, 1750, 1861, 2055, 2070, 2254
LmnI GCTCC 3 cut(s) 1045, 1769, 2110
Lsp1109I GCAGC 4 cut(s) 778, 802, 2269, 2341
LweI GCATC 5 cut(s) 1179, 1204, 1879, 2210, 2318
MaeI CTAG 9 cut(s) 159, 290, 636, 1077, 1193, 1418, 1428, 1709, 1886
MaeII ACGT 1 cut(s) 1481
MaeIII GTNAC 6 cut(s) 543, 676, 833, 869, 1545, 2413
MalI GATC 9 cut(s) 479, 654, 1227, 1443, 1752, 1863, 2057, 2072, 2256
MboI GATC 9 cut(s) 477, 652, 1225, 1441, 1750, 1861, 2055, 2070, 2254
MboII GAAGA 9 cut(s) 133, 1399, 1619, 1703, 1817, 1874, 2031, 2168, 2264
MfeI CAATTG 2 cut(s) 38, 1949
MflI RGATCY 3 cut(s) 1225, 1861, 2254
MhlI GDGCHC 4 cut(s) 47, 1161, 1209, 2238
MlsI TGGCCA 1 cut(s) 1557
MluNI TGGCCA 1 cut(s) 1557
MlyI GAGTC 4 cut(s) 356, 1635, 1730, 1829
MmeI TCCRAC 2 cut(s) 964, 1669
MnlI CCTC 8 cut(s) 35, 38, 265, 733, 1290, 1377, 1511, 2101
Mox20I TGGCCA 1 cut(s) 1557
Mph1103I ATGCAT 1 cut(s) 2225
MroXI GAANNNNTTC 2 cut(s) 1120, 2023
MscI TGGCCA 1 cut(s) 1557
MseI TTAA 7 cut(s) 441, 624, 632, 885, 2400, 2408, 2437
MslI CAYNNNNRTG 2 cut(s) 168, 1212
Msp20I TGGCCA 1 cut(s) 1557
MspA1I CMGCKG 1 cut(s) 2282
MspI CCGG 2 cut(s) 756, 829
MspR9I CCNGG 9 cut(s) 52, 416, 483, 558, 657, 757, 912, 1559, 1832
MunI CAATTG 2 cut(s) 38, 1949
Mva1269I GAATGC 2 cut(s) 2113, 2223
MvaI CCWGG 8 cut(s) 52, 416, 483, 558, 657, 912, 1559, 1832
MvnI CGCG 2 cut(s) 1066, 1812
MwoI GCNNNNNNNGC 2 cut(s) 1809, 1973
NciI CCSGG 1 cut(s) 757
NdeI CATATG 1 cut(s) 1984
NdeII GATC 9 cut(s) 477, 652, 1225, 1441, 1750, 1861, 2055, 2070, 2254
NlaIII CATG 9 cut(s) 69, 780, 1179, 1264, 1306, 1720, 2122, 2278, 2296
NlaIV GGNNCC 6 cut(s) 101, 856, 1041, 1655, 1946, 1998
NmuCI GTSAC 3 cut(s) 543, 833, 2413
NsiI ATGCAT 1 cut(s) 2225
NspI RCATGY 2 cut(s) 1306, 1720
PaeI GCATGC 1 cut(s) 1306
PaeR7I CTCGAG 1 cut(s) 2430
PctI GAATGC 2 cut(s) 2113, 2223
PdmI GAANNNNTTC 2 cut(s) 1120, 2023
PfeI GAWTC 5 cut(s) 592, 752, 943, 1900, 2180
PinAI ACCGGT 1 cut(s) 828
PkrI GCNGC 4 cut(s) 768, 817, 2284, 2331
PleI GAGTC 4 cut(s) 355, 1635, 1730, 1829
PpsI GAGTC 4 cut(s) 355, 1635, 1730, 1829
PpuMI RGGWCCY 1 cut(s) 100
PshBI ATTAAT 1 cut(s) 885
PsiI TTATAA 1 cut(s) 1536
Psp5II RGGWCCY 1 cut(s) 100
Psp6I CCWGG 8 cut(s) 50, 414, 481, 556, 655, 910, 1557, 1830
PspGI CCWGG 8 cut(s) 50, 414, 481, 556, 655, 910, 1557, 1830
PspN4I GGNNCC 6 cut(s) 101, 856, 1041, 1655, 1946, 1998
PspPI GGNCC 4 cut(s) 100, 659, 1945, 2263
PspPPI RGGWCCY 1 cut(s) 100
PstNI CAGNNNCTG 1 cut(s) 2285
PsuI RGATCY 3 cut(s) 1225, 1861, 2254
PvuII CAGCTG 1 cut(s) 2282
RsaI GTAC 2 cut(s) 1081, 1344
RsaNI GTAC 2 cut(s) 1080, 1343
RseI CAYNNNNRTG 2 cut(s) 168, 1212
SaqAI TTAA 7 cut(s) 441, 624, 632, 885, 2400, 2408, 2437
SatI GCNGC 4 cut(s) 767, 816, 2283, 2330
Sau3AI GATC 9 cut(s) 477, 652, 1225, 1441, 1750, 1861, 2055, 2070, 2254
Sau96I GGNCC 4 cut(s) 100, 659, 1945, 2263
SchI GAGTC 4 cut(s) 356, 1635, 1730, 1829
ScrFI CCNGG 9 cut(s) 52, 416, 483, 558, 657, 757, 912, 1559, 1832
SduI GDGCHC 4 cut(s) 47, 1161, 1209, 2238
SfaNI GCATC 5 cut(s) 1179, 1204, 1879, 2210, 2318
SfcI CTRYAG 5 cut(s) 465, 571, 577, 859, 2189
Sfr274I CTCGAG 1 cut(s) 2430
SinI GGWCC 3 cut(s) 100, 1945, 2263
SlaI CTCGAG 1 cut(s) 2430
SmiMI CAYNNNNRTG 2 cut(s) 168, 1212
SmlI CTYRAG 3 cut(s) 1837, 2215, 2430
SmoI CTYRAG 3 cut(s) 1837, 2215, 2430
SpeI ACTAGT 1 cut(s) 1076
SphI GCATGC 1 cut(s) 1306
SsiI CCGC 3 cut(s) 166, 1409, 1812
SspI AATATT 3 cut(s) 883, 929, 2064
SspMI CTAG 9 cut(s) 159, 290, 636, 1077, 1193, 1418, 1428, 1709, 1886
StyD4I CCNGG 9 cut(s) 50, 414, 481, 556, 655, 755, 910, 1557, 1830
StyI CCWWGG 2 cut(s) 1767, 2166
TaaI ACNGT 6 cut(s) 214, 794, 849, 1201, 2193, 2413
TaiI ACGT 1 cut(s) 1484
TaqI TCGA 1 cut(s) 2431
TaqII GACCGA 1 cut(s) 2280
TatI WGTACW 1 cut(s) 1079
TfiI GAWTC 5 cut(s) 592, 752, 943, 1900, 2180
Tru1I TTAA 7 cut(s) 441, 624, 632, 885, 2400, 2408, 2437
Tru9I TTAA 7 cut(s) 441, 624, 632, 885, 2400, 2408, 2437
TscAI CASTG 5 cut(s) 219, 593, 854, 1410, 2418
TseFI GTSAC 3 cut(s) 543, 833, 2413
TseI GCWGC 4 cut(s) 766, 815, 2282, 2329
Tsp45I GTSAC 3 cut(s) 543, 833, 2413
TspGWI ACGGA 1 cut(s) 2396
TspRI CASTG 5 cut(s) 219, 593, 854, 1410, 2418
VneI GTGCAC 1 cut(s) 43
VpaK11BI GGWCC 3 cut(s) 100, 1945, 2263
VspI ATTAAT 1 cut(s) 885
XapI RAATTY 4 cut(s) 177, 1610, 1908, 1932
XbaI TCTAGA 1 cut(s) 1885
XceI RCATGY 2 cut(s) 1306, 1720
XhoI CTCGAG 1 cut(s) 2430
XmiI GTMKAC 1 cut(s) 332
XmnI GAANNNNTTC 2 cut(s) 1120, 2023
XspI CTAG 9 cut(s) 159, 290, 636, 1077, 1193, 1418, 1428, 1709, 1886
Zsp2I ATGCAT 1 cut(s) 2225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.