Rw0G002360

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00072
OG0000112
Physical Location & Seq
Forward (+)
222741 .. 229139
6399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G002360.1

Sequence Viewer

Length: 2649 bp
ATGGAATTCGTCCACGATTACATGACAAACAGACAAAGAGGACACCACCCAATTAACCGCTACAGGACAAAGTTGACAACCGGAAACAGGCTAAGCCTTTTCCATCTCCAATTACTATTAATCAACTTACACGTGCATATCACGTGTCTGGGCCCAAACACTCCGAGCATGGAGAGCTCCGAGCAGCGAAGACGTTCTCCCAATAATACCCCGAAAATGTTATTGAAATCTTTGCTTCTCTTCCTCCTCTTCCAATTTTGCACTTGCAGGGACACCATCAGATGGAATGAACAACAACTGAAGGATGTTGATGGTGAAGTTTTGGTGTCCAAAGAAAACAACTTTGAGTTGGGTTTCTTCAGCCCCGGCGATTCTAGCCACCGGTACGTTGGTATTTGGTATTCTCAATCTAAAATATCTGCAAAAACAGTGGTGTGGATCGCAAACAGAGACAGCCCCGTCAATGATACCTCGGGAGTTCTCACAATGAACAGGTATGGAGAATTAGCTCTTTATGCTTATAACATGGAGAACATTCCTATATGGTCCACAAATGCGTCACAGTCGGTCCAAAACACAGACACCTTATATAGATATGTACAGCTTTTAGATACTGGAAACTTGGTTGTGTTCCGGGATGGTAGAAATGGAAGCTTGATTTTGTGGCAAAGTTTTGATTATCCTACAGATACTCTAATCGCAGGTATGAAATCTGGTTTGAATTGGCAAAAAGGGCTAGATTGGCATTTAACATCTTGGAAGTCACAAGATGACCCTGGAAAGGGGGACTATGCCTTTAGGGTCGATCCGAATCACAATGCTACTCCCCAATATTTTGTCTACAAGGGTTACACTAAGTATTGGCGAATGGATGCAGGGCCATGGCCTAATTTTGTCAGTAATGCCAACGAAATGTATAATACTCTGCCTTATAACAGCAATGCTATTTCAATAGTAAGGCTGAAGGATTCCGGGTTACTTCAGCACCTAATATGGAATGATGCTGACCATCAATGGGTGGAAATGTGGTCTTCGCCGAAATACCCTTGTGACTGGTATGGACATTGTGGTGCTAACAGCAAATGTAGCCCTGACAATATTTATTACTTTGAGTGTGAGTGTATTCCAGGGTATGAGCCAAAATCTGTAGATGGGTGGAATCAGAGAAATGGAACTGATGGATGTGTGAGTAATCGAGTAAGTGTGTCAAAGTGTAGGAATGGAGAAGGGTTTGTGAAGGTGGCAAGGGTAAAAGATCCAGACACATCGAATGTAGCAAGGTTGAGAACAAGTATGAGTGCCAAAGAGTGTGAGCAGGAGTGCTTGAAAAATTGTTCTTGCACGGCTTATATGAGCATTACACTGGAAAGGGTCGTCAATTGCTTGACATGGTTTGATGACTTGATGGACATGTTAGTTTACACAAAGCTCGGACGAGATCTATATGTTCGTGTGGATAAAATCTCATTAGCTGAGGCCAGAAACTCACAAGGTTTCTTCAGGAGTGGCAAGGTCCTTATTCCAATACTAACTGCTTTGGTGGCACTACTACTAATCATTATACTTTCCTTGTGGTTGCTTAAGAAAAAGAGGAAGACAAGAGACTATTTGGATGCGGAGGAACTTGAGGAAAGTAAGAGACACCCGGAATTGCAATTTTTCGATCTTAACACACTAATTGCAGCCACAGACAACTTCTCTGATGCCAATAAACTTGGCCAAGGTGGTTTTGGCATTGTTTATAAGGGTGAGTTACCAAACGAACAAATAGTTGCTATAAAAAGATTGTCCAGAACTTCAGGACAGGGAATCGAAGAATTCAAAAACGAAGTTGCACTAATAGCCAGACTTCAACACAGGAATCTTGTGAAACTTTTAGGCTGCTGTATAAAGGGAGAAGAAAGGATATTGGTTCTGGAATACATGCCGAACAAAAGCTTGGACTCCTTTCTTTTTGATAACACAAGGAGGTCACTCTTAAATTGGGAAAAGCGTTTTGAAATTATCAATGGGGTTGCTCGTGGGGTTCTATATCTTCATCGGGACTCAAGATTGAGGATTATCCACAGGGATCTGAAAACCAGTAATGTCCTTTTAGAGAGCGAGTTAGACCCAAAAATTTCTGATTTCGGCATGGCAAGAATATTTCAAGGGGACCAACTCCTGGAAAAGACAAACAGAGTTGTCGGAACATATGGCTACATGTCACCAGAATATGCAATATTTGGGAGATTTTCTACGAAATCTGATGTGTTTAGTTTTGGGATCATACTATTGGAGATTGTTAGTGGCAAGAGAAACAATGGTTCTTATGAGATGCATTATTCCATAAACTTGATAGGACATGTATGGCAACTTTGGGTAGAAGACAAAGTCTTGGAAATTGTGGATTCATCACTAGAGATTTATCAGCTTGATGAAGTCTTGAGATGCATTCAAGTTGGGCTTTTGTGTGTGCAAGAAGATTTGAAGGACCGACCAGCCATGTCAGCCGTCGTGTGCATGTTGAGTGGTGAAACATCTCTTCCATCTCCTAAGCAGCCTGCATTTGTTTTCAGAACAAGTTCCAGCATTGTTGATGATCCCTTCATTCCAAATGAATCATATTCTAGTAATAGTTTGACGATAACTAACATGGAAGCGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

882

Amino Acids

101.05

Weight (kDa)

7.0

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 143 - 254 2.6e-31 D-mannose binding lectin
S_locus_glycop PF00954 298 - 382 2.9e-14 S-locus glycoprotein domain
PAN_2 PF08276 405 - 471 4e-16 PAN-like domain
Pkinase PF00069 567 - 768 1.5e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 570 - 835 2.3e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 839 - 882 4.1e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 522, 933, 1743
AasI GACNNNNNNGTC 1 cut(s) 458
Acc36I ACCTGC 1 cut(s) 692
AccB7I CCANNNNNTGG 2 cut(s) 282, 2164
AccI GTMKAC 1 cut(s) 840
AccII CGCG 1 cut(s) 2644
AciI CCGC 2 cut(s) 58, 1616
AclWI GGATC 6 cut(s) 446, 800, 1250, 2079, 2273, 2576
AcoI YGGCCR 1 cut(s) 1717
AcsI RAATTY 3 cut(s) 5, 1817, 2118
AcuI CTGAAG 6 cut(s) 320, 343, 965, 983, 1483, 1782
AcvI CACGTG 2 cut(s) 133, 144
AfaI GTAC 2 cut(s) 386, 600
AfiI CCNNNNNNNGG 6 cut(s) 87, 282, 781, 782, 1015, 2164
AflII CTTAAG 1 cut(s) 1580
AflIII ACRYGT 5 cut(s) 130, 143, 1410, 2202, 2344
AgeI ACCGGT 1 cut(s) 381
AjnI CCWGG 3 cut(s) 775, 1126, 2163
AjuI GAANNNNNNNTTGG 2 cut(s) 1922, 1954
AluBI AGCT 8 cut(s) 177, 509, 604, 654, 1429, 1472, 1938, 2413
AluI AGCT 8 cut(s) 177, 509, 604, 654, 1429, 1472, 1938, 2413
Alw21I GWGCWC 1 cut(s) 179
Alw26I GTCTC 3 cut(s) 444, 1596, 1633
AlwI GGATC 6 cut(s) 446, 800, 1250, 2079, 2273, 2576
Ama87I CYCGRG 1 cut(s) 472
AoxI GGCC 5 cut(s) 151, 878, 884, 1476, 1717
ApaI GGGCCC 1 cut(s) 155
ApeKI GCWGC 4 cut(s) 184, 1682, 1881, 2539
ApoI RAATTY 3 cut(s) 5, 1817, 2118
AseI ATTAAT 1 cut(s) 119
AsiGI ACCGGT 1 cut(s) 381
Asp700I GAANNNNTTC 2 cut(s) 193, 2563
AspLEI GCGC 1 cut(s) 2644
AspS9I GGNCC 8 cut(s) 151, 152, 546, 568, 878, 1513, 2155, 2473
AsuC2I CCSGG 4 cut(s) 366, 635, 973, 1646
AsuHPI GGTGA 4 cut(s) 326, 1760, 2199, 2525
AvaI CYCGRG 1 cut(s) 472
AvaII GGWCC 5 cut(s) 546, 568, 1513, 2155, 2473
BaeGI GKGCMC 1 cut(s) 155
BaeI ACNNNNGTAYC 2 cut(s) 376, 409
BalI TGGCCA 1 cut(s) 1719
BanII GRGCYC 2 cut(s) 155, 179
BauI CACGAG 1 cut(s) 2019
BbrPI CACGTG 2 cut(s) 133, 144
BbsI GAAGAC 4 cut(s) 196, 1023, 1601, 2373
Bbv12I GWGCWC 1 cut(s) 179
BbvCI CCTCAGC 1 cut(s) 1473
BbvI GCAGC 4 cut(s) 196, 1694, 1868, 2551
BceAI ACGGC 2 cut(s) 1359, 2477
BciT130I CCWGG 3 cut(s) 777, 1128, 2165
BcnI CCSGG 4 cut(s) 366, 635, 973, 1646
BcoDI GTCTC 3 cut(s) 444, 1596, 1633
BfaI CTAG 4 cut(s) 375, 737, 2399, 2610
BfmI CTRYAG 3 cut(s) 61, 684, 1146
BfrI CTTAAG 1 cut(s) 1580
BfuAI ACCTGC 1 cut(s) 692
BglII AGATCT 1 cut(s) 1438
BisI GCNGC 4 cut(s) 185, 1683, 1882, 2540
BlpI GCTNAGC 1 cut(s) 92
BlsI GCNGC 4 cut(s) 186, 1684, 1883, 2541
Bme1390I CCNGG 7 cut(s) 366, 635, 777, 973, 1128, 1646, 2165
Bme18I GGWCC 5 cut(s) 546, 568, 1513, 2155, 2473
BmeT110I CYCGRG 1 cut(s) 472
BmgT120I GGNCC 8 cut(s) 151, 152, 546, 568, 878, 1513, 2155, 2473
BmiI GGNNCC 2 cut(s) 153, 2156
BmrFI CCNGG 7 cut(s) 366, 635, 777, 973, 1128, 1646, 2165
BmsI GCATC 6 cut(s) 862, 991, 1603, 1693, 2307, 2420
BpiI GAAGAC 4 cut(s) 196, 1023, 1601, 2373
Bpu10I CCTNAGC 2 cut(s) 1473, 2535
Bpu1102I GCTNAGC 1 cut(s) 92
BpuEI CTTGAG 3 cut(s) 1646, 2032, 2446
BpuMI CCSGG 4 cut(s) 366, 635, 973, 1646
BsaAI YACGTR 2 cut(s) 133, 144
BsaBI GATNNNNATC 1 cut(s) 810
BsaJI CCNNGG 6 cut(s) 364, 471, 775, 881, 1127, 1720
BsaWI WCCGGW 2 cut(s) 80, 381
Bsc4I CCNNNNNNNGG 6 cut(s) 87, 282, 781, 782, 1015, 2164
Bse118I RCCGGY 1 cut(s) 381
Bse1I ACTGG 4 cut(s) 619, 1058, 1368, 2082
Bse3DI GCAATG 1 cut(s) 946
Bse8I GATNNNNATC 1 cut(s) 810
BseBI CCWGG 3 cut(s) 777, 1128, 2165
BseDI CCNNGG 6 cut(s) 364, 471, 775, 881, 1127, 1720
BseGI GGATG 5 cut(s) 310, 643, 877, 1187, 1618
BseJI GATNNNNATC 1 cut(s) 810
BseLI CCNNNNNNNGG 6 cut(s) 87, 282, 781, 782, 1015, 2164
BseMI GCAATG 1 cut(s) 946
BseMII CTCAG 1 cut(s) 1464
BseNI ACTGG 4 cut(s) 619, 1058, 1368, 2082
BseRI GAGGAG 1 cut(s) 236
BseSI GKGCMC 1 cut(s) 155
BseXI GCAGC 4 cut(s) 196, 1694, 1868, 2551
Bsh1236I CGCG 1 cut(s) 2644
BshFI GGCC 5 cut(s) 153, 880, 886, 1478, 1719
BshTI ACCGGT 1 cut(s) 381
BsiHKAI GWGCWC 1 cut(s) 179
BsiHKCI CYCGRG 1 cut(s) 472
BsiSI CCGG 6 cut(s) 81, 366, 382, 634, 972, 1646
BslFI GGGAC 4 cut(s) 284, 800, 2057, 2168
BslI CCNNNNNNNGG 6 cut(s) 87, 282, 781, 782, 1015, 2164
BsmAI GTCTC 3 cut(s) 444, 1596, 1633
BsmFI GGGAC 4 cut(s) 284, 800, 2057, 2168
BsmI GAATGC 1 cut(s) 2433
BsnI GGCC 5 cut(s) 153, 880, 886, 1478, 1719
BsoBI CYCGRG 1 cut(s) 472
Bsp120I GGGCCC 1 cut(s) 151
Bsp1286I GDGCHC 2 cut(s) 155, 179
Bsp1407I TGTACA 1 cut(s) 598
Bsp143I GATC 8 cut(s) 438, 805, 1255, 1438, 1663, 2071, 2265, 2581
Bsp1720I GCTNAGC 1 cut(s) 92
Bsp19I CCATGG 1 cut(s) 881
BspACI CCGC 2 cut(s) 58, 1616
BspANI GGCC 5 cut(s) 153, 880, 886, 1478, 1719
BspCNI CTCAG 1 cut(s) 1465
BspFNI CGCG 1 cut(s) 2644
BspLI GGNNCC 2 cut(s) 153, 2156
BspMI ACCTGC 1 cut(s) 692
BspPI GGATC 6 cut(s) 446, 800, 1250, 2079, 2273, 2576
BspTI CTTAAG 1 cut(s) 1580
BsrDI GCAATG 1 cut(s) 946
BsrFI RCCGGY 1 cut(s) 381
BsrGI TGTACA 1 cut(s) 598
BsrI ACTGG 4 cut(s) 619, 1058, 1368, 2082
BssAI RCCGGY 1 cut(s) 381
BssECI CCNNGG 6 cut(s) 364, 471, 775, 881, 1127, 1720
BssMI GATC 8 cut(s) 438, 805, 1255, 1438, 1663, 2071, 2265, 2581
BssSI CACGAG 1 cut(s) 2019
BssT1I CCWWGG 2 cut(s) 881, 1720
Bst2BI CACGAG 1 cut(s) 2019
Bst2UI CCWGG 3 cut(s) 777, 1128, 2165
Bst4CI ACNGT 2 cut(s) 430, 564
Bst6I CTCTTC 3 cut(s) 245, 254, 2529
BstAFI CTTAAG 1 cut(s) 1580
BstAUI TGTACA 1 cut(s) 598
BstBAI YACGTR 2 cut(s) 133, 144
BstC8I GCNNGC 1 cut(s) 2544
BstDEI CTNAG 4 cut(s) 92, 855, 1473, 2535
BstDSI CCRYGG 1 cut(s) 881
BstF5I GGATG 5 cut(s) 310, 643, 877, 1187, 1618
BstFNI CGCG 1 cut(s) 2644
BstHHI GCGC 1 cut(s) 2644
BstKTI GATC 8 cut(s) 441, 808, 1258, 1441, 1666, 2074, 2268, 2584
BstMAI GTCTC 3 cut(s) 444, 1596, 1633
BstMBI GATC 8 cut(s) 438, 805, 1255, 1438, 1663, 2071, 2265, 2581
BstMWI GCNNNNNNNGC 9 cut(s) 174, 375, 515, 733, 742, 1086, 1541, 1841, 2489
BstNI CCWGG 3 cut(s) 777, 1128, 2165
BstNSI RCATGY 5 cut(s) 1414, 1927, 2206, 2348, 2506
BstSCI CCNGG 7 cut(s) 364, 633, 775, 971, 1126, 1644, 2163
BstSFI CTRYAG 3 cut(s) 61, 684, 1146
BstSLI GKGCMC 1 cut(s) 155
BstUI CGCG 1 cut(s) 2644
BstV1I GCAGC 4 cut(s) 196, 1694, 1868, 2551
BstV2I GAAGAC 4 cut(s) 196, 1023, 1601, 2373
BstX2I RGATCY 3 cut(s) 1255, 1438, 2071
BstYI RGATCY 3 cut(s) 1255, 1438, 2071
BsuRI GGCC 5 cut(s) 153, 880, 886, 1478, 1719
BtgI CCRYGG 1 cut(s) 881
BtsCI GGATG 5 cut(s) 310, 643, 877, 1187, 1618
BtsIMutI CAGTG 2 cut(s) 435, 1361
BveI ACCTGC 1 cut(s) 692
Cac8I GCNNGC 1 cut(s) 2544
CfoI GCGC 1 cut(s) 2644
Cfr10I RCCGGY 1 cut(s) 381
Cfr13I GGNCC 8 cut(s) 151, 152, 546, 568, 878, 1513, 2155, 2473
CseI GACGC 1 cut(s) 546
Csp6I GTAC 2 cut(s) 385, 599
CspAI ACCGGT 1 cut(s) 381
CspCI CAANNNNNGTGG 2 cut(s) 411, 446
CviQI GTAC 2 cut(s) 385, 599
DdeI CTNAG 4 cut(s) 92, 855, 1473, 2535
DpnI GATC 8 cut(s) 440, 807, 1257, 1440, 1665, 2073, 2267, 2583
DpnII GATC 8 cut(s) 438, 805, 1255, 1438, 1663, 2071, 2265, 2581
DrdI GACNNNNNNGTC 1 cut(s) 458
DseDI GACNNNNNNGTC 1 cut(s) 458
EaeI YGGCCR 1 cut(s) 1717
Eam1104I CTCTTC 3 cut(s) 245, 254, 2529
EarI CTCTTC 3 cut(s) 245, 254, 2529
Ecl136II GAGCTC 1 cut(s) 177
Eco130I CCWWGG 2 cut(s) 881, 1720
Eco24I GRGCYC 2 cut(s) 155, 179
Eco47I GGWCC 5 cut(s) 546, 568, 1513, 2155, 2473
Eco53kI GAGCTC 1 cut(s) 177
Eco57I CTGAAG 6 cut(s) 320, 343, 965, 983, 1483, 1782
Eco72I CACGTG 2 cut(s) 133, 144
Eco88I CYCGRG 1 cut(s) 472
EcoICRI GAGCTC 1 cut(s) 177
EcoO109I RGGNCCY 1 cut(s) 1513
EcoRI GAATTC 2 cut(s) 5, 1817
EcoRII CCWGG 3 cut(s) 775, 1126, 2163
EcoT14I CCWWGG 2 cut(s) 881, 1720
EcoT22I ATGCAT 2 cut(s) 2322, 2435
EcoT38I GRGCYC 2 cut(s) 155, 179
ErhI CCWWGG 2 cut(s) 881, 1720
FalI AAGNNNNNCTT 2 cut(s) 2430, 2462
FaqI GGGAC 4 cut(s) 284, 800, 2057, 2168
FauNDI CATATG 1 cut(s) 2194
FblI GTMKAC 1 cut(s) 840
Fnu4HI GCNGC 4 cut(s) 185, 1683, 1882, 2540
FokI GGATG 5 cut(s) 317, 650, 884, 1194, 1625
FriOI GRGCYC 2 cut(s) 155, 179
Fsp4HI GCNGC 4 cut(s) 185, 1683, 1882, 2540
FspBI CTAG 4 cut(s) 375, 737, 2399, 2610
GlaI GCGC 1 cut(s) 2643
GluI GCNGC 4 cut(s) 185, 1683, 1882, 2540
HaeIII GGCC 5 cut(s) 153, 880, 886, 1478, 1719
HapII CCGG 6 cut(s) 81, 366, 382, 634, 972, 1646
HgaI GACGC 1 cut(s) 546
HhaI GCGC 1 cut(s) 2644
Hin6I GCGC 1 cut(s) 2642
HinP1I GCGC 1 cut(s) 2642
HincII GTYRAC 1 cut(s) 75
HindII GTYRAC 1 cut(s) 75
HindIII AAGCTT 2 cut(s) 652, 1936
HpaII CCGG 6 cut(s) 81, 366, 382, 634, 972, 1646
HphI GGTGA 4 cut(s) 326, 1760, 2199, 2525
Hpy166II GTNNAC 5 cut(s) 13, 75, 549, 841, 1420
Hpy188III TCNNGA 9 cut(s) 474, 1259, 1501, 1791, 1800, 1916, 2042, 2049, 2425
Hpy8I GTNNAC 5 cut(s) 13, 75, 549, 841, 1420
Hpy99I CGWCG 1 cut(s) 2498
HpyAV CCTTC 6 cut(s) 295, 958, 1220, 1231, 2464, 2596
HpyCH4III ACNGT 2 cut(s) 430, 564
HpyCH4IV ACGT 4 cut(s) 132, 143, 193, 387
HpyF10VI GCNNNNNNNGC 9 cut(s) 174, 375, 515, 733, 742, 1086, 1541, 1841, 2489
HpyF3I CTNAG 4 cut(s) 92, 855, 1473, 2535
HpySE526I ACGT 4 cut(s) 132, 143, 193, 387
HspAI GCGC 1 cut(s) 2642
Kzo9I GATC 8 cut(s) 438, 805, 1255, 1438, 1663, 2071, 2265, 2581
LmnI GCTCC 1 cut(s) 182
Lsp1109I GCAGC 4 cut(s) 196, 1694, 1868, 2551
LweI GCATC 6 cut(s) 862, 991, 1603, 1693, 2307, 2420
MaeI CTAG 4 cut(s) 375, 737, 2399, 2610
MaeII ACGT 4 cut(s) 132, 143, 193, 387
MaeIII GTNAC 8 cut(s) 558, 762, 848, 975, 1049, 1752, 1971, 2205
MalI GATC 8 cut(s) 440, 807, 1257, 1440, 1665, 2073, 2267, 2583
MboI GATC 8 cut(s) 438, 805, 1255, 1438, 1663, 2071, 2265, 2581
MfeI CAATTG 1 cut(s) 1378
MflI RGATCY 3 cut(s) 1255, 1438, 2071
MhlI GDGCHC 2 cut(s) 155, 179
MlsI TGGCCA 1 cut(s) 1719
MluNI TGGCCA 1 cut(s) 1719
MlyI GAGTC 2 cut(s) 1937, 2039
MmeI TCCRAC 1 cut(s) 2167
Mox20I TGGCCA 1 cut(s) 1719
Mph1103I ATGCAT 2 cut(s) 2322, 2435
MroXI GAANNNNTTC 2 cut(s) 193, 2563
MscI TGGCCA 1 cut(s) 1719
MseI TTAA 6 cut(s) 54, 119, 749, 1581, 1668, 1979
MslI CAYNNNNRTG 1 cut(s) 1068
Msp20I TGGCCA 1 cut(s) 1719
MspCI CTTAAG 1 cut(s) 1580
MspI CCGG 6 cut(s) 81, 366, 382, 634, 972, 1646
MspR9I CCNGG 7 cut(s) 366, 635, 777, 973, 1128, 1646, 2165
MunI CAATTG 1 cut(s) 1378
Mva1269I GAATGC 1 cut(s) 2433
MvaI CCWGG 3 cut(s) 777, 1128, 2165
MvnI CGCG 1 cut(s) 2644
MwoI GCNNNNNNNGC 9 cut(s) 174, 375, 515, 733, 742, 1086, 1541, 1841, 2489
NciI CCSGG 4 cut(s) 366, 635, 973, 1646
NcoI CCATGG 1 cut(s) 881
NdeI CATATG 1 cut(s) 2194
NdeII GATC 8 cut(s) 438, 805, 1255, 1438, 1663, 2071, 2265, 2581
NlaIV GGNNCC 2 cut(s) 153, 2156
NmuCI GTSAC 5 cut(s) 558, 762, 1049, 1971, 2205
NsiI ATGCAT 2 cut(s) 2322, 2435
NspI RCATGY 5 cut(s) 1414, 1927, 2206, 2348, 2506
PciI ACATGT 3 cut(s) 1410, 2202, 2344
PctI GAATGC 1 cut(s) 2433
PdmI GAANNNNTTC 2 cut(s) 193, 2563
PfeI GAWTC 8 cut(s) 371, 811, 968, 1159, 1809, 1861, 2390, 2600
PflFI GACNNNGTC 1 cut(s) 2372
PflMI CCANNNNNTGG 2 cut(s) 282, 2164
PfoI TCCNGGA 2 cut(s) 633, 2163
PinAI ACCGGT 1 cut(s) 381
PkrI GCNGC 4 cut(s) 186, 1684, 1883, 2541
PleI GAGTC 2 cut(s) 1937, 2039
PmaCI CACGTG 2 cut(s) 133, 144
PmlI CACGTG 2 cut(s) 133, 144
PpsI GAGTC 2 cut(s) 1937, 2039
Ppu21I YACGTR 2 cut(s) 133, 144
PpuMI RGGWCCY 1 cut(s) 1513
PscI ACATGT 3 cut(s) 1410, 2202, 2344
PshBI ATTAAT 1 cut(s) 119
PsiI TTATAA 3 cut(s) 522, 933, 1743
Psp124BI GAGCTC 1 cut(s) 179
Psp5II RGGWCCY 1 cut(s) 1513
Psp6I CCWGG 3 cut(s) 775, 1126, 2163
PspCI CACGTG 2 cut(s) 133, 144
PspGI CCWGG 3 cut(s) 775, 1126, 2163
PspN4I GGNNCC 2 cut(s) 153, 2156
PspOMI GGGCCC 1 cut(s) 151
PspPI GGNCC 8 cut(s) 151, 152, 546, 568, 878, 1513, 2155, 2473
PspPPI RGGWCCY 1 cut(s) 1513
PsuI RGATCY 3 cut(s) 1255, 1438, 2071
PsyI GACNNNGTC 1 cut(s) 2372
RsaI GTAC 2 cut(s) 386, 600
RsaNI GTAC 2 cut(s) 385, 599
RseI CAYNNNNRTG 1 cut(s) 1068
SacI GAGCTC 1 cut(s) 179
SaqAI TTAA 6 cut(s) 54, 119, 749, 1581, 1668, 1979
SatI GCNGC 4 cut(s) 185, 1683, 1882, 2540
Sau3AI GATC 8 cut(s) 438, 805, 1255, 1438, 1663, 2071, 2265, 2581
Sau96I GGNCC 8 cut(s) 151, 152, 546, 568, 878, 1513, 2155, 2473
SchI GAGTC 2 cut(s) 1937, 2039
ScrFI CCNGG 7 cut(s) 366, 635, 777, 973, 1128, 1646, 2165
SduI GDGCHC 2 cut(s) 155, 179
SfaNI GCATC 6 cut(s) 862, 991, 1603, 1693, 2307, 2420
SfcI CTRYAG 3 cut(s) 61, 684, 1146
SinI GGWCC 5 cut(s) 546, 568, 1513, 2155, 2473
SmiMI CAYNNNNRTG 1 cut(s) 1068
SmlI CTYRAG 4 cut(s) 1580, 1625, 2047, 2425
SmoI CTYRAG 4 cut(s) 1580, 1625, 2047, 2425
SsiI CCGC 2 cut(s) 58, 1616
SspI AATATT 4 cut(s) 833, 1099, 2145, 2223
SspMI CTAG 4 cut(s) 375, 737, 2399, 2610
SstI GAGCTC 1 cut(s) 179
StyD4I CCNGG 7 cut(s) 364, 633, 775, 971, 1126, 1644, 2163
StyI CCWWGG 2 cut(s) 881, 1720
TaaI ACNGT 2 cut(s) 430, 564
TaiI ACGT 4 cut(s) 135, 146, 196, 390
TaqI TCGA 5 cut(s) 804, 1195, 1268, 1662, 1812
TaqII GACCGA 2 cut(s) 556, 2490
TatI WGTACW 1 cut(s) 598
TfiI GAWTC 8 cut(s) 371, 811, 968, 1159, 1809, 1861, 2390, 2600
Tru1I TTAA 6 cut(s) 54, 119, 749, 1581, 1668, 1979
Tru9I TTAA 6 cut(s) 54, 119, 749, 1581, 1668, 1979
TscAI CASTG 2 cut(s) 435, 1368
TseFI GTSAC 5 cut(s) 558, 762, 1049, 1971, 2205
TseI GCWGC 4 cut(s) 184, 1682, 1881, 2539
Tsp45I GTSAC 5 cut(s) 558, 762, 1049, 1971, 2205
TspDTI ATGAA 8 cut(s) 303, 503, 722, 2027, 2382, 2433, 2578, 2613
TspRI CASTG 2 cut(s) 435, 1368
Tth111I GACNNNGTC 1 cut(s) 2372
Van91I CCANNNNNTGG 2 cut(s) 282, 2164
Vha464I CTTAAG 1 cut(s) 1580
VpaK11BI GGWCC 5 cut(s) 546, 568, 1513, 2155, 2473
VspI ATTAAT 1 cut(s) 119
XapI RAATTY 3 cut(s) 5, 1817, 2118
XceI RCATGY 5 cut(s) 1414, 1927, 2206, 2348, 2506
XcmI CCANNNNNNNNNTGG 2 cut(s) 386, 1727
XmiI GTMKAC 1 cut(s) 840
XmnI GAANNNNTTC 2 cut(s) 193, 2563
XspI CTAG 4 cut(s) 375, 737, 2399, 2610
Zsp2I ATGCAT 2 cut(s) 2322, 2435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.