serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 159 |
| AccB1I | GGYRCC | 1 cut(s) | 603 |
| AccI | GTMKAC | 1 cut(s) | 153 |
| AccII | CGCG | 2 cut(s) | 112, 198 |
| AccIII | TCCGGA | 1 cut(s) | 969 |
| AciI | CCGC | 9 cut(s) | 4, 24, 87, 110, 120, 232, 597, 684, 789 |
| AclWI | GGATC | 2 cut(s) | 886, 961 |
| AcuI | CTGAAG | 4 cut(s) | 306, 791, 902, 1002 |
| AcyI | GRCGYC | 1 cut(s) | 156 |
| AfaI | GTAC | 4 cut(s) | 388, 511, 899, 923 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 66, 116, 231, 744, 939 |
| AflIII | ACRYGT | 2 cut(s) | 451, 620 |
| AgeI | ACCGGT | 1 cut(s) | 900 |
| AgsI | TTSAA | 2 cut(s) | 31, 252 |
| AjiI | CACGTC | 1 cut(s) | 287 |
| AjnI | CCWGG | 1 cut(s) | 132 |
| AleI | CACNNNNGTG | 1 cut(s) | 1031 |
| AluBI | AGCT | 7 cut(s) | 139, 311, 320, 563, 586, 656, 986 |
| AluI | AGCT | 7 cut(s) | 139, 311, 320, 563, 586, 656, 986 |
| Alw21I | GWGCWC | 3 cut(s) | 141, 502, 1089 |
| Alw26I | GTCTC | 3 cut(s) | 40, 208, 746 |
| AlwI | GGATC | 2 cut(s) | 886, 961 |
| Ama87I | CYCGRG | 1 cut(s) | 125 |
| Aor13HI | TCCGGA | 1 cut(s) | 969 |
| AoxI | GGCC | 3 cut(s) | 122, 143, 746 |
| ApaI | GGGCCC | 1 cut(s) | 126 |
| ApeKI | GCWGC | 7 cut(s) | 70, 106, 308, 479, 575, 653, 818 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 425, 457 |
| AsiGI | ACCGGT | 1 cut(s) | 900 |
| AspLEI | GCGC | 3 cut(s) | 75, 114, 200 |
| AspS9I | GGNCC | 8 cut(s) | 122, 123, 130, 144, 181, 260, 746, 930 |
| AsuHPI | GGTGA | 2 cut(s) | 311, 824 |
| AvaI | CYCGRG | 1 cut(s) | 125 |
| AvaII | GGWCC | 4 cut(s) | 130, 181, 260, 930 |
| BaeGI | GKGCMC | 2 cut(s) | 126, 795 |
| BaeI | ACNNNNGTAYC | 1 cut(s) | 32 |
| BanI | GGYRCC | 1 cut(s) | 603 |
| BanII | GRGCYC | 2 cut(s) | 126, 141 |
| BbsI | GAAGAC | 1 cut(s) | 897 |
| Bbv12I | GWGCWC | 3 cut(s) | 141, 502, 1089 |
| BbvCI | CCTCAGC | 1 cut(s) | 687 |
| BbvI | GCAGC | 7 cut(s) | 57, 93, 320, 466, 562, 665, 830 |
| BccI | CCATC | 2 cut(s) | 60, 1031 |
| BceAI | ACGGC | 3 cut(s) | 37, 83, 185 |
| BciT130I | CCWGG | 1 cut(s) | 134 |
| BcoDI | GTCTC | 3 cut(s) | 40, 208, 746 |
| BfmI | CTRYAG | 4 cut(s) | 91, 339, 564, 576 |
| BlpI | GCTNAGC | 1 cut(s) | 663 |
| BmcAI | AGTACT | 1 cut(s) | 923 |
| Bme1390I | CCNGG | 1 cut(s) | 134 |
| Bme18I | GGWCC | 4 cut(s) | 130, 181, 260, 930 |
| BmeT110I | CYCGRG | 1 cut(s) | 125 |
| BmgBI | CACGTC | 1 cut(s) | 287 |
| BmgT120I | GGNCC | 8 cut(s) | 122, 123, 130, 144, 181, 260, 746, 930 |
| BmiI | GGNNCC | 2 cut(s) | 124, 605 |
| BmrFI | CCNGG | 1 cut(s) | 134 |
| BmsI | GCATC | 3 cut(s) | 376, 712, 830 |
| BpiI | GAAGAC | 1 cut(s) | 897 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1027, 1059 |
| BpmI | CTGGAG | 1 cut(s) | 155 |
| Bpu10I | CCTNAGC | 1 cut(s) | 687 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 663 |
| BpuEI | CTTGAG | 2 cut(s) | 152, 1014 |
| BsaHI | GRCGYC | 1 cut(s) | 156 |
| BsaJI | CCNNGG | 3 cut(s) | 60, 99, 126 |
| BsaWI | WCCGGW | 2 cut(s) | 900, 969 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 309, 339, 549, 579 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 66, 116, 231, 744, 939 |
| Bse118I | RCCGGY | 1 cut(s) | 900 |
| Bse1I | ACTGG | 3 cut(s) | 860, 895, 929 |
| Bse3DI | GCAATG | 1 cut(s) | 362 |
| BseAI | TCCGGA | 1 cut(s) | 969 |
| BseBI | CCWGG | 1 cut(s) | 134 |
| BseDI | CCNNGG | 3 cut(s) | 60, 99, 126 |
| BseGI | GGATG | 1 cut(s) | 367 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 66, 116, 231, 744, 939 |
| BseMI | GCAATG | 1 cut(s) | 362 |
| BseMII | CTCAG | 4 cut(s) | 516, 654, 678, 963 |
| BseNI | ACTGG | 3 cut(s) | 860, 895, 929 |
| BseRI | GAGGAG | 1 cut(s) | 573 |
| BseSI | GKGCMC | 2 cut(s) | 126, 795 |
| BseXI | GCAGC | 7 cut(s) | 57, 93, 320, 466, 562, 665, 830 |
| Bsh1236I | CGCG | 2 cut(s) | 112, 198 |
| BshFI | GGCC | 3 cut(s) | 124, 145, 748 |
| BshNI | GGYRCC | 1 cut(s) | 603 |
| BshTI | ACCGGT | 1 cut(s) | 900 |
| BsiHKAI | GWGCWC | 3 cut(s) | 141, 502, 1089 |
| BsiHKCI | CYCGRG | 1 cut(s) | 125 |
| BsiSI | CCGG | 3 cut(s) | 142, 901, 970 |
| BslFI | GGGAC | 4 cut(s) | 34, 143, 297, 345 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 66, 116, 231, 744, 939 |
| BsmAI | GTCTC | 3 cut(s) | 40, 208, 746 |
| BsmBI | CGTCTC | 2 cut(s) | 208, 746 |
| BsmFI | GGGAC | 4 cut(s) | 34, 143, 297, 345 |
| BsmI | GAATGC | 1 cut(s) | 234 |
| BsnI | GGCC | 3 cut(s) | 124, 145, 748 |
| BsoBI | CYCGRG | 1 cut(s) | 125 |
| Bsp120I | GGGCCC | 1 cut(s) | 122 |
| Bsp1286I | GDGCHC | 5 cut(s) | 126, 141, 502, 795, 1089 |
| Bsp13I | TCCGGA | 1 cut(s) | 969 |
| Bsp143I | GATC | 4 cut(s) | 163, 640, 891, 966 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 663 |
| BspACI | CCGC | 9 cut(s) | 4, 24, 87, 110, 120, 232, 597, 684, 789 |
| BspANI | GGCC | 3 cut(s) | 124, 145, 748 |
| BspCNI | CTCAG | 4 cut(s) | 517, 655, 679, 962 |
| BspEI | TCCGGA | 1 cut(s) | 969 |
| BspFNI | CGCG | 2 cut(s) | 112, 198 |
| BspHI | TCATGA | 2 cut(s) | 936, 1045 |
| BspLI | GGNNCC | 2 cut(s) | 124, 605 |
| BspMAI | CTGCAG | 3 cut(s) | 95, 343, 580 |
| BspPI | GGATC | 2 cut(s) | 886, 961 |
| BspT107I | GGYRCC | 1 cut(s) | 603 |
| BsrDI | GCAATG | 1 cut(s) | 362 |
| BsrFI | RCCGGY | 1 cut(s) | 900 |
| BsrI | ACTGG | 3 cut(s) | 860, 895, 929 |
| BssAI | RCCGGY | 1 cut(s) | 900 |
| BssECI | CCNNGG | 3 cut(s) | 60, 99, 126 |
| BssMI | GATC | 4 cut(s) | 163, 640, 891, 966 |
| BssNI | GRCGYC | 1 cut(s) | 156 |
| BssT1I | CCWWGG | 1 cut(s) | 60 |
| Bst2UI | CCWGG | 1 cut(s) | 134 |
| BstACI | GRCGYC | 1 cut(s) | 156 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 659 |
| BstC8I | GCNNGC | 6 cut(s) | 91, 122, 498, 588, 669, 791 |
| BstDEI | CTNAG | 6 cut(s) | 11, 525, 541, 663, 687, 949 |
| BstDSI | CCRYGG | 1 cut(s) | 99 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 937 |
| BstF5I | GGATG | 1 cut(s) | 367 |
| BstFNI | CGCG | 2 cut(s) | 112, 198 |
| BstHHI | GCGC | 3 cut(s) | 75, 114, 200 |
| BstKTI | GATC | 4 cut(s) | 166, 643, 894, 969 |
| BstMAI | GTCTC | 3 cut(s) | 40, 208, 746 |
| BstMBI | GATC | 4 cut(s) | 163, 640, 891, 966 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 95, 317, 488, 497, 596, 659, 668, 1049 |
| BstNI | CCWGG | 1 cut(s) | 134 |
| BstNSI | RCATGY | 2 cut(s) | 455, 624 |
| BstSCI | CCNGG | 1 cut(s) | 132 |
| BstSFI | CTRYAG | 4 cut(s) | 91, 339, 564, 576 |
| BstSLI | GKGCMC | 2 cut(s) | 126, 795 |
| BstUI | CGCG | 2 cut(s) | 112, 198 |
| BstV1I | GCAGC | 7 cut(s) | 57, 93, 320, 466, 562, 665, 830 |
| BstV2I | GAAGAC | 1 cut(s) | 897 |
| BstX2I | RGATCY | 2 cut(s) | 891, 966 |
| BstYI | RGATCY | 2 cut(s) | 891, 966 |
| BsuRI | GGCC | 3 cut(s) | 124, 145, 748 |
| BtgI | CCRYGG | 1 cut(s) | 99 |
| BtgZI | GCGATG | 1 cut(s) | 64 |
| BtrI | CACGTC | 1 cut(s) | 287 |
| BtsCI | GGATG | 1 cut(s) | 367 |
| Cac8I | GCNNGC | 6 cut(s) | 91, 122, 498, 588, 669, 791 |
| CciI | TCATGA | 2 cut(s) | 936, 1045 |
| CfoI | GCGC | 3 cut(s) | 75, 114, 200 |
| Cfr10I | RCCGGY | 1 cut(s) | 900 |
| Cfr13I | GGNCC | 8 cut(s) | 122, 123, 130, 144, 181, 260, 746, 930 |
| CseI | GACGC | 1 cut(s) | 429 |
| Csp6I | GTAC | 4 cut(s) | 387, 510, 898, 922 |
| CspAI | ACCGGT | 1 cut(s) | 900 |
| CviAII | CATG | 9 cut(s) | 452, 608, 621, 694, 911, 937, 962, 1046, 1073 |
| CviQI | GTAC | 4 cut(s) | 387, 510, 898, 922 |
| DdeI | CTNAG | 6 cut(s) | 11, 525, 541, 663, 687, 949 |
| DpnI | GATC | 4 cut(s) | 165, 642, 893, 968 |
| DpnII | GATC | 4 cut(s) | 163, 640, 891, 966 |
| DraI | TTTAAA | 1 cut(s) | 780 |
| Ecl136II | GAGCTC | 1 cut(s) | 139 |
| Eco130I | CCWWGG | 1 cut(s) | 60 |
| Eco24I | GRGCYC | 2 cut(s) | 126, 141 |
| Eco32I | GATATC | 1 cut(s) | 522 |
| Eco47I | GGWCC | 4 cut(s) | 130, 181, 260, 930 |
| Eco53kI | GAGCTC | 1 cut(s) | 139 |
| Eco57I | CTGAAG | 4 cut(s) | 306, 791, 902, 1002 |
| Eco88I | CYCGRG | 1 cut(s) | 125 |
| EcoICRI | GAGCTC | 1 cut(s) | 139 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 937 |
| EcoO109I | RGGNCCY | 2 cut(s) | 130, 181 |
| EcoRII | CCWGG | 1 cut(s) | 132 |
| EcoRV | GATATC | 1 cut(s) | 522 |
| EcoT14I | CCWWGG | 1 cut(s) | 60 |
| EcoT38I | GRGCYC | 2 cut(s) | 126, 141 |
| ErhI | CCWWGG | 1 cut(s) | 60 |
| Esp3I | CGTCTC | 2 cut(s) | 208, 746 |
| FaeI | CATG | 9 cut(s) | 455, 611, 624, 697, 914, 940, 965, 1049, 1076 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 408, 440 |
| FaqI | GGGAC | 4 cut(s) | 34, 143, 297, 345 |
| FatI | CATG | 9 cut(s) | 451, 607, 620, 693, 910, 936, 961, 1045, 1072 |
| FauI | CCCGC | 2 cut(s) | 113, 782 |
| FblI | GTMKAC | 1 cut(s) | 153 |
| FokI | GGATG | 1 cut(s) | 354 |
| FriOI | GRGCYC | 2 cut(s) | 126, 141 |
| GlaI | GCGC | 3 cut(s) | 74, 113, 199 |
| GsuI | CTGGAG | 1 cut(s) | 155 |
| HaeIII | GGCC | 3 cut(s) | 124, 145, 748 |
| HapII | CCGG | 3 cut(s) | 142, 901, 970 |
| HgaI | GACGC | 1 cut(s) | 429 |
| HhaI | GCGC | 3 cut(s) | 75, 114, 200 |
| Hin1I | GRCGYC | 1 cut(s) | 156 |
| Hin1II | CATG | 9 cut(s) | 455, 611, 624, 697, 914, 940, 965, 1049, 1076 |
| Hin6I | GCGC | 3 cut(s) | 73, 112, 198 |
| HinP1I | GCGC | 3 cut(s) | 73, 112, 198 |
| HincII | GTYRAC | 3 cut(s) | 154, 486, 676 |
| HindII | GTYRAC | 3 cut(s) | 154, 486, 676 |
| HinfI | GANTC | 3 cut(s) | 47, 947, 1069 |
| HpaII | CCGG | 3 cut(s) | 142, 901, 970 |
| HphI | GGTGA | 2 cut(s) | 311, 824 |
| Hpy166II | GTNNAC | 4 cut(s) | 154, 486, 676, 930 |
| Hpy188I | TCNGA | 5 cut(s) | 372, 415, 526, 952, 982 |
| Hpy188III | TCNNGA | 3 cut(s) | 937, 970, 1046 |
| Hpy8I | GTNNAC | 4 cut(s) | 154, 486, 676, 930 |
| Hpy99I | CGWCG | 1 cut(s) | 158 |
| HpyAV | CCTTC | 2 cut(s) | 943, 947 |
| HpyCH4IV | ACGT | 2 cut(s) | 156, 286 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 95, 317, 488, 497, 596, 659, 668, 1049 |
| HpyF3I | CTNAG | 6 cut(s) | 11, 525, 541, 663, 687, 949 |
| HpySE526I | ACGT | 2 cut(s) | 156, 286 |
| Hsp92I | GRCGYC | 1 cut(s) | 156 |
| Hsp92II | CATG | 9 cut(s) | 455, 611, 624, 697, 914, 940, 965, 1049, 1076 |
| HspAI | GCGC | 3 cut(s) | 73, 112, 198 |
| Kpn2I | TCCGGA | 1 cut(s) | 969 |
| Kzo9I | GATC | 4 cut(s) | 163, 640, 891, 966 |
| LmnI | GCTCC | 4 cut(s) | 136, 144, 317, 560 |
| Lsp1109I | GCAGC | 7 cut(s) | 57, 93, 320, 466, 562, 665, 830 |
| LweI | GCATC | 3 cut(s) | 376, 712, 830 |
| MaeII | ACGT | 2 cut(s) | 156, 286 |
| MaeIII | GTNAC | 3 cut(s) | 15, 610, 957 |
| MalI | GATC | 4 cut(s) | 165, 642, 893, 968 |
| MboI | GATC | 4 cut(s) | 163, 640, 891, 966 |
| MboII | GAAGA | 4 cut(s) | 428, 895, 897, 1072 |
| MfeI | CAATTG | 1 cut(s) | 849 |
| MflI | RGATCY | 2 cut(s) | 891, 966 |
| MhlI | GDGCHC | 5 cut(s) | 126, 141, 502, 795, 1089 |
| MluCI | AATT | 4 cut(s) | 276, 405, 444, 849 |
| MlyI | GAGTC | 2 cut(s) | 56, 941 |
| MmeI | TCCRAC | 1 cut(s) | 395 |
| MroI | TCCGGA | 1 cut(s) | 969 |
| MseI | TTAA | 3 cut(s) | 327, 779, 995 |
| MslI | CAYNNNNRTG | 3 cut(s) | 698, 1004, 1031 |
| MspA1I | CMGCKG | 3 cut(s) | 311, 656, 686 |
| MspI | CCGG | 3 cut(s) | 142, 901, 970 |
| MspR9I | CCNGG | 1 cut(s) | 134 |
| MunI | CAATTG | 1 cut(s) | 849 |
| Mva1269I | GAATGC | 1 cut(s) | 234 |
| MvaI | CCWGG | 1 cut(s) | 134 |
| MvnI | CGCG | 2 cut(s) | 112, 198 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 95, 317, 488, 497, 596, 659, 668, 1049 |
| NdeII | GATC | 4 cut(s) | 163, 640, 891, 966 |
| NlaIII | CATG | 9 cut(s) | 455, 611, 624, 697, 914, 940, 965, 1049, 1076 |
| NlaIV | GGNNCC | 2 cut(s) | 124, 605 |
| NmuCI | GTSAC | 1 cut(s) | 15 |
| NspI | RCATGY | 2 cut(s) | 455, 624 |
| OliI | CACNNNNGTG | 1 cut(s) | 1031 |
| PagI | TCATGA | 2 cut(s) | 936, 1045 |
| PciI | ACATGT | 2 cut(s) | 451, 620 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 123 |
| PctI | GAATGC | 1 cut(s) | 234 |
| PfeI | GAWTC | 1 cut(s) | 1069 |
| PfoI | TCCNGGA | 1 cut(s) | 132 |
| PinAI | ACCGGT | 1 cut(s) | 900 |
| PleI | GAGTC | 2 cut(s) | 55, 941 |
| PpsI | GAGTC | 2 cut(s) | 55, 941 |
| PpuMI | RGGWCCY | 2 cut(s) | 130, 181 |
| PscI | ACATGT | 2 cut(s) | 451, 620 |
| Psp124BI | GAGCTC | 1 cut(s) | 141 |
| Psp5II | RGGWCCY | 2 cut(s) | 130, 181 |
| Psp6I | CCWGG | 1 cut(s) | 132 |
| PspGI | CCWGG | 1 cut(s) | 132 |
| PspN4I | GGNNCC | 2 cut(s) | 124, 605 |
| PspOMI | GGGCCC | 1 cut(s) | 122 |
| PspPI | GGNCC | 8 cut(s) | 122, 123, 130, 144, 181, 260, 746, 930 |
| PspPPI | RGGWCCY | 2 cut(s) | 130, 181 |
| PstI | CTGCAG | 3 cut(s) | 95, 343, 580 |
| PsuI | RGATCY | 2 cut(s) | 891, 966 |
| PvuII | CAGCTG | 2 cut(s) | 311, 656 |
| RsaI | GTAC | 4 cut(s) | 388, 511, 899, 923 |
| RsaNI | GTAC | 4 cut(s) | 387, 510, 898, 922 |
| RseI | CAYNNNNRTG | 3 cut(s) | 698, 1004, 1031 |
| SacI | GAGCTC | 1 cut(s) | 141 |
| SalI | GTCGAC | 1 cut(s) | 152 |
| SaqAI | TTAA | 3 cut(s) | 327, 779, 995 |
| Sau3AI | GATC | 4 cut(s) | 163, 640, 891, 966 |
| Sau96I | GGNCC | 8 cut(s) | 122, 123, 130, 144, 181, 260, 746, 930 |
| ScaI | AGTACT | 1 cut(s) | 923 |
| SchI | GAGTC | 2 cut(s) | 56, 941 |
| ScrFI | CCNGG | 1 cut(s) | 134 |
| SduI | GDGCHC | 5 cut(s) | 126, 141, 502, 795, 1089 |
| SfaNI | GCATC | 3 cut(s) | 376, 712, 830 |
| SfcI | CTRYAG | 4 cut(s) | 91, 339, 564, 576 |
| SinI | GGWCC | 4 cut(s) | 130, 181, 260, 930 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 698, 1004, 1031 |
| SmlI | CTYRAG | 2 cut(s) | 167, 1029 |
| SmoI | CTYRAG | 2 cut(s) | 167, 1029 |
| Sse9I | AATT | 4 cut(s) | 276, 405, 444, 849 |
| SsiI | CCGC | 9 cut(s) | 4, 24, 87, 110, 120, 232, 597, 684, 789 |
| SspI | AATATT | 1 cut(s) | 255 |
| SstI | GAGCTC | 1 cut(s) | 141 |
| StyD4I | CCNGG | 1 cut(s) | 132 |
| StyI | CCWWGG | 1 cut(s) | 60 |
| TaiI | ACGT | 2 cut(s) | 159, 289 |
| TaqI | TCGA | 3 cut(s) | 153, 643, 1089 |
| TasI | AATT | 4 cut(s) | 276, 405, 444, 849 |
| TatI | WGTACW | 1 cut(s) | 921 |
| TauI | GCSGC | 3 cut(s) | 27, 112, 599 |
| TfiI | GAWTC | 1 cut(s) | 1069 |
| Tru1I | TTAA | 3 cut(s) | 327, 779, 995 |
| Tru9I | TTAA | 3 cut(s) | 327, 779, 995 |
| TseFI | GTSAC | 1 cut(s) | 15 |
| TseI | GCWGC | 7 cut(s) | 70, 106, 308, 479, 575, 653, 818 |
| Tsp45I | GTSAC | 1 cut(s) | 15 |
| TspDTI | ATGAA | 3 cut(s) | 435, 925, 1061 |
| TspGWI | ACGGA | 1 cut(s) | 770 |
| VpaK11BI | GGWCC | 4 cut(s) | 130, 181, 260, 930 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 937 |
| XceI | RCATGY | 2 cut(s) | 455, 624 |
| XmiI | GTMKAC | 1 cut(s) | 153 |
| ZraI | GACGTC | 1 cut(s) | 157 |
| ZrmI | AGTACT | 1 cut(s) | 923 |