Rorug05G0224700

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000112
Physical Location & Seq
Reverse (-)
23288893 .. 23290994
2102 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0224700.1

Sequence Viewer

Length: 1986 bp
ATGGCTGTCTGCTGTTATGTTCTCCAGTTCTCACCAGAGCGGCTTCAGTTCCTTCTTCTTCTCTTGTTTGTTTTAACCTTGTCTGCAACTCCATTGACTTTCAACTTCTCCAGCTTTCCCGCCAACACCAGGACTCTATTTCTAGAGGGAGATGCTTTCACCGACGGCTCCCTCCGACTCACCAAAAGTGCTCTTGATGACGCAAGGCACTGGAGCACCGGTAGAGCCACCTTCGGTGAATCGTTCCTCCTCCGGCAGAATGAAAAACTAGCTGATTTCACTTCAAGTTTCATATTCTCCATTAACACCTCCAATGACACCTTCTGCGGTGACGGTTTGGTGTTCTTCCTAGCTCCAAACGGCTCCTTACTCGACCAGGCCTTAGGAAGAGGTAGCAAACTTGGCCTCCCTGTCAACACTACGCTGCCAAACTTTACCACCAGGTACTCGTTTGTGGCAGTCGAGTTTGATATCTACCCTAATAGGCATACTTCAGGCCAACCAATAATCGAAGATCCTGATTACGATCATGGGGGATCGACATCAACTCTGTCAAATCGTTACAGCAAAGTACAGGTGATCAAAAAAATTTATTGCTTGATTGATCTGCTTCAATACTTGCCCGATAGGGTCATTGTTGGATTCTCTGCTTCCACAGGTGCTGCAGTTGCTCTGCATAAGATCAATGCATGGAATTTTACCTCCACTCTTCTGGAGGATGATAATTCGACAAACAACAATCCTCTGGCTCGCAATAGTGCGACGACGTCCAACAACAACGTAGGTCTAGCAGTCGGATTGGGTGTTGGTGGATGTGTTACCTTGGTTGGTGCATTGGCTTTTGTTTGGTACATCTTCTGCACGAAGAAGAGGGAAACAGCTGGGGAAAGTACTGATGATGATACTTTGGATATTCATGATTTGGTTCCTGATGAATTCGAAAAGGGGATAGGCCCCAGGAAGTTTTCCTACAATGAATTGGCCCAAGCAACTAACAATTTTGCACAAGGAGAAAAGCTTGGGGAAGGAGGATTTGGCCGAGTTTATAGAGGCTGCATCAAAGATTTGGACTCATATGTTGCTGTTAAGAAGATATCCCGAGGGTCTAAACAAGGGTTGAAGGAGTATGCAGCAGAAGTAAGTATCATCAGTCGGCTGAGGCATCGAAATCTAGTGCAACTAATTGGTTGGTGCCACGAAAAGAAACTCCTACTTGTCTACGAATACATGCCCAACGGAAGCTTATATACTCTTCTGTTCAAAGAAGAAAGCATGTTAACTTGGGAGGTAAGGTACAAAATTGCAAATGGATTGGCCGCAGGATTGCTCTATCTACACCAAGAATGGGAACAGTGTGTGCTGCACAGGGATATCAAATCCAGCAATGTTATGTTGGACTCGAATTTCAATGCCAAACTTGGAGATTTTGGGTTAGCTCGACTTGTAGACCATGGAAAAATGTCTCGAACTACGATTGTAGCAGGAACAATGGGCTACATGGCTATGGAATATGTTACAACGGGGAAGGCTAGCAAGGAAACAGATGTCTATAGTTTCGGAGTTGTTGCTTTGGAAATAGCTTGTGGGAGAAAACCCATTGATTCGAGGTATGAAGAGAAGCAAGACAATATGGTGGAGTGGGTTTGGGAGCTCTATGGAGATGGGAAAATTATCGAAGCAGCTGATCAGAAACTGCGTGGAGATTTTGATGTGAAACAAATGGAGTGCTTATTGATGGTTGGGTTGTGGTGCGCTCGTCCGGATTACAATTTCAGGCCTTTGATACAAGAAGCAATTCGAGTGCTTAACTTCGAAGTTCCATTGCCCGTTCTCCCACCAAAGATGCCAATGGTTTTTGGTCTTCCATTATCACACCCAACGTTGTCCAATTGCGACACTACTGGTGGTCAAAGTGAGCCATCCATCAATTCTTCACTGTTCACCTCATCTTCTACAACAAACTCTAATCCATCACCAACATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

661

Amino Acids

73.37

Weight (kDa)

5.69

Isoelectric Point (pI)

29.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 32 - 162 3.1e-22 Legume lectin domain
Lectin_legB PF00139 194 - 245 5.8e-09 Legume lectin domain
Pkinase PF00069 335 - 541 4e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 336 - 539 2.8e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 770
AccB1I GGYRCC 1 cut(s) 1191
AccBSI CCGCTC 1 cut(s) 40
AccI GTMKAC 2 cut(s) 1218, 1446
AccIII TCCGGA 1 cut(s) 1759
AciI CCGC 4 cut(s) 40, 120, 327, 1317
AclI AACGTT 1 cut(s) 1880
AclWI GGATC 2 cut(s) 509, 544
AcoI YGGCCR 2 cut(s) 1036, 1314
AcsI RAATTY 4 cut(s) 588, 694, 935, 1402
AcuI CTGAAG 2 cut(s) 29, 477
AcyI GRCGYC 1 cut(s) 767
AfaI GTAC 5 cut(s) 446, 573, 851, 892, 1295
AfiI CCNNNNNNNGG 2 cut(s) 129, 1345
AgeI ACCGGT 1 cut(s) 218
AgsI TTSAA 6 cut(s) 103, 285, 614, 1120, 1261, 1408
AjnI CCWGG 4 cut(s) 128, 375, 440, 956
AjuI GAANNNNNNNTTGG 4 cut(s) 305, 337, 1017, 1049
Alw21I GWGCWC 3 cut(s) 193, 218, 1653
Alw26I GTCTC 1 cut(s) 1467
AlwI GGATC 2 cut(s) 509, 544
AlwNI CAGNNNCTG 2 cut(s) 662, 1693
Ama87I CYCGRG 1 cut(s) 1098
Aor13HI TCCGGA 1 cut(s) 1759
AoxI GGCC 8 cut(s) 378, 403, 496, 952, 981, 1036, 1314, 1775
ApeKI GCWGC 6 cut(s) 424, 662, 1053, 1130, 1360, 1679
ApoI RAATTY 4 cut(s) 588, 694, 935, 1402
AsiGI ACCGGT 1 cut(s) 218
Asp700I GAANNNNTTC 1 cut(s) 1794
AspLEI GCGC 1 cut(s) 1754
AspS9I GGNCC 2 cut(s) 953, 982
AsuHPI GGTGA 8 cut(s) 24, 151, 172, 248, 341, 589, 1933, 1965
AsuII TTCGAA 2 cut(s) 939, 1812
AsuNHI GCTAGC 1 cut(s) 1529
AvaI CYCGRG 1 cut(s) 1098
AxyI CCTNAGG 1 cut(s) 382
BanI GGYRCC 1 cut(s) 1191
BanII GRGCYC 1 cut(s) 1653
BarI GAAGNNNNNNTAC 4 cut(s) 953, 985, 1231, 1263
BbsI GAAGAC 1 cut(s) 1853
Bbv12I GWGCWC 3 cut(s) 193, 218, 1653
BbvCI CCTCAGC 1 cut(s) 1157
BbvI GCAGC 6 cut(s) 411, 649, 1040, 1142, 1347, 1691
BccI CCATC 5 cut(s) 1655, 1729, 1927, 1931, 1978
BceAI ACGGC 2 cut(s) 181, 376
BciT130I CCWGG 4 cut(s) 130, 377, 442, 958
BclI TGATCA 2 cut(s) 579, 1684
BcoDI GTCTC 1 cut(s) 1467
BfaI CTAG 6 cut(s) 143, 269, 350, 788, 1172, 1530
BfmI CTRYAG 2 cut(s) 663, 1549
BisI GCNGC 8 cut(s) 41, 425, 663, 1054, 1131, 1317, 1361, 1680
BlsI GCNGC 8 cut(s) 42, 426, 664, 1055, 1132, 1318, 1362, 1681
BmcAI AGTACT 1 cut(s) 892
Bme1390I CCNGG 4 cut(s) 130, 377, 442, 958
BmeT110I CYCGRG 1 cut(s) 1098
BmgT120I GGNCC 2 cut(s) 953, 982
BmiI GGNNCC 5 cut(s) 169, 364, 927, 955, 1193
BmrFI CCNGG 4 cut(s) 130, 377, 442, 958
BmsI GCATC 4 cut(s) 142, 1065, 1171, 1833
BmtI GCTAGC 1 cut(s) 1533
BpiI GAAGAC 1 cut(s) 1853
BpmI CTGGAG 4 cut(s) 8, 94, 232, 734
Bpu10I CCTNAGC 1 cut(s) 1157
Bpu14I TTCGAA 2 cut(s) 939, 1812
BsaBI GATNNNNATC 2 cut(s) 525, 541
BsaHI GRCGYC 1 cut(s) 767
BsaJI CCNNGG 4 cut(s) 822, 956, 1099, 1450
BsaWI WCCGGW 2 cut(s) 218, 1759
BsaXI ACNNNNNCTCC 6 cut(s) 205, 235, 390, 420, 1277, 1307
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 1345
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 3 cut(s) 25, 215, 1906
Bse21I CCTNAGG 1 cut(s) 382
Bse3DI GCAATG 2 cut(s) 1390, 1820
Bse8I GATNNNNATC 2 cut(s) 525, 541
BseAI TCCGGA 1 cut(s) 1759
BseBI CCWGG 4 cut(s) 130, 377, 442, 958
BseDI CCNNGG 4 cut(s) 822, 956, 1099, 1450
BseGI GGATG 3 cut(s) 724, 818, 1919
BseJI GATNNNNATC 2 cut(s) 525, 541
BseLI CCNNNNNNNGG 2 cut(s) 129, 1345
BseMI GCAATG 2 cut(s) 1390, 1820
BseMII CTCAG 1 cut(s) 1148
BseNI ACTGG 3 cut(s) 25, 215, 1906
BseRI GAGGAG 1 cut(s) 239
BseXI GCAGC 6 cut(s) 411, 649, 1040, 1142, 1347, 1691
BseYI CCCAGC 1 cut(s) 881
BsgI GTGCAG 2 cut(s) 844, 1346
BshFI GGCC 8 cut(s) 380, 405, 498, 954, 983, 1038, 1316, 1777
BshNI GGYRCC 1 cut(s) 1191
BshTI ACCGGT 1 cut(s) 218
BsiHKAI GWGCWC 3 cut(s) 193, 218, 1653
BsiHKCI CYCGRG 1 cut(s) 1098
BsiSI CCGG 3 cut(s) 219, 253, 1760
BslI CCNNNNNNNGG 2 cut(s) 129, 1345
BsmAI GTCTC 1 cut(s) 1467
BsnI GGCC 8 cut(s) 380, 405, 498, 954, 983, 1038, 1316, 1777
BsoBI CYCGRG 1 cut(s) 1098
Bsp119I TTCGAA 2 cut(s) 939, 1812
Bsp1286I GDGCHC 3 cut(s) 193, 218, 1653
Bsp13I TCCGGA 1 cut(s) 1759
Bsp143I GATC 7 cut(s) 514, 526, 536, 579, 604, 681, 1684
Bsp19I CCATGG 1 cut(s) 1450
BspACI CCGC 4 cut(s) 40, 120, 327, 1317
BspANI GGCC 8 cut(s) 380, 405, 498, 954, 983, 1038, 1316, 1777
BspCNI CTCAG 1 cut(s) 1149
BspEI TCCGGA 1 cut(s) 1759
BspHI TCATGA 1 cut(s) 916
BspLI GGNNCC 5 cut(s) 169, 364, 927, 955, 1193
BspMAI CTGCAG 1 cut(s) 667
BspOI GCTAGC 1 cut(s) 1533
BspPI GGATC 2 cut(s) 509, 544
BspT104I TTCGAA 2 cut(s) 939, 1812
BspT107I GGYRCC 1 cut(s) 1191
BsrBI CCGCTC 1 cut(s) 40
BsrDI GCAATG 2 cut(s) 1390, 1820
BsrFI RCCGGY 1 cut(s) 218
BsrI ACTGG 3 cut(s) 25, 215, 1906
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 4 cut(s) 822, 956, 1099, 1450
BssMI GATC 7 cut(s) 514, 526, 536, 579, 604, 681, 1684
BssNI GRCGYC 1 cut(s) 767
BssT1I CCWWGG 2 cut(s) 822, 1450
Bst2UI CCWGG 4 cut(s) 130, 377, 442, 958
Bst4CI ACNGT 3 cut(s) 335, 1353, 1938
Bst6I CTCTTC 5 cut(s) 382, 714, 863, 1257, 1608
BstACI GRCGYC 1 cut(s) 767
BstBI TTCGAA 2 cut(s) 939, 1812
BstC8I GCNNGC 2 cut(s) 751, 1531
BstDEI CTNAG 2 cut(s) 382, 1157
BstDSI CCRYGG 1 cut(s) 1450
BstF5I GGATG 3 cut(s) 724, 818, 1919
BstHHI GCGC 1 cut(s) 1754
BstKTI GATC 7 cut(s) 517, 529, 539, 582, 607, 684, 1687
BstMAI GTCTC 1 cut(s) 1467
BstMBI GATC 7 cut(s) 514, 526, 536, 579, 604, 681, 1684
BstMWI GCNNNNNNNGC 2 cut(s) 402, 668
BstNI CCWGG 4 cut(s) 130, 377, 442, 958
BstNSI RCATGY 2 cut(s) 1231, 1276
BstSCI CCNGG 4 cut(s) 128, 375, 440, 956
BstSFI CTRYAG 2 cut(s) 663, 1549
BstV1I GCAGC 6 cut(s) 411, 649, 1040, 1142, 1347, 1691
BstV2I GAAGAC 1 cut(s) 1853
BstX2I RGATCY 1 cut(s) 514
BstXI CCANNNNNNTGG 1 cut(s) 712
BstYI RGATCY 1 cut(s) 514
Bsu36I CCTNAGG 1 cut(s) 382
BsuRI GGCC 8 cut(s) 380, 405, 498, 954, 983, 1038, 1316, 1777
BtgI CCRYGG 1 cut(s) 1450
BtsCI GGATG 3 cut(s) 724, 818, 1919
BtsIMutI CAGTG 3 cut(s) 208, 1358, 1934
Cac8I GCNNGC 2 cut(s) 751, 1531
CaiI CAGNNNCTG 2 cut(s) 662, 1693
CciI TCATGA 1 cut(s) 916
CfoI GCGC 1 cut(s) 1754
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 2 cut(s) 953, 982
CseI GACGC 1 cut(s) 209
CsiI ACCWGGT 1 cut(s) 440
Csp6I GTAC 5 cut(s) 445, 572, 850, 891, 1294
CspAI ACCGGT 1 cut(s) 218
CviAII CATG 7 cut(s) 530, 690, 917, 1228, 1273, 1451, 1498
CviQI GTAC 5 cut(s) 445, 572, 850, 891, 1294
DdeI CTNAG 2 cut(s) 382, 1157
DpnI GATC 7 cut(s) 516, 528, 538, 581, 606, 683, 1686
DpnII GATC 7 cut(s) 514, 526, 536, 579, 604, 681, 1684
EaeI YGGCCR 2 cut(s) 1036, 1314
Eam1104I CTCTTC 5 cut(s) 382, 714, 863, 1257, 1608
EarI CTCTTC 5 cut(s) 382, 714, 863, 1257, 1608
Ecl136II GAGCTC 1 cut(s) 1651
Eco130I CCWWGG 2 cut(s) 822, 1450
Eco147I AGGCCT 2 cut(s) 380, 1777
Eco24I GRGCYC 1 cut(s) 1653
Eco32I GATATC 3 cut(s) 472, 1095, 1372
Eco53kI GAGCTC 1 cut(s) 1651
Eco57I CTGAAG 2 cut(s) 29, 477
Eco81I CCTNAGG 1 cut(s) 382
Eco88I CYCGRG 1 cut(s) 1098
EcoICRI GAGCTC 1 cut(s) 1651
EcoO109I RGGNCCY 1 cut(s) 953
EcoRI GAATTC 1 cut(s) 935
EcoRII CCWGG 4 cut(s) 128, 375, 440, 956
EcoRV GATATC 3 cut(s) 472, 1095, 1372
EcoT14I CCWWGG 2 cut(s) 822, 1450
EcoT22I ATGCAT 1 cut(s) 691
EcoT38I GRGCYC 1 cut(s) 1653
ErhI CCWWGG 2 cut(s) 822, 1450
FaeI CATG 7 cut(s) 533, 693, 920, 1231, 1276, 1454, 1501
FatI CATG 7 cut(s) 529, 689, 916, 1227, 1272, 1450, 1497
FauI CCCGC 1 cut(s) 127
FauNDI CATATG 1 cut(s) 1075
FbaI TGATCA 2 cut(s) 579, 1684
FblI GTMKAC 2 cut(s) 1218, 1446
Fnu4HI GCNGC 8 cut(s) 41, 425, 663, 1054, 1131, 1317, 1361, 1680
FokI GGATG 3 cut(s) 731, 825, 1906
FriOI GRGCYC 1 cut(s) 1653
Fsp4HI GCNGC 8 cut(s) 41, 425, 663, 1054, 1131, 1317, 1361, 1680
FspBI CTAG 6 cut(s) 143, 269, 350, 788, 1172, 1530
GlaI GCGC 1 cut(s) 1753
GluI GCNGC 8 cut(s) 41, 425, 663, 1054, 1131, 1317, 1361, 1680
GsaI CCCAGC 1 cut(s) 885
GsuI CTGGAG 4 cut(s) 8, 94, 232, 734
HaeIII GGCC 8 cut(s) 380, 405, 498, 954, 983, 1038, 1316, 1777
HapII CCGG 3 cut(s) 219, 253, 1760
HgaI GACGC 1 cut(s) 209
HhaI GCGC 1 cut(s) 1754
Hin1I GRCGYC 1 cut(s) 767
Hin1II CATG 7 cut(s) 533, 693, 920, 1231, 1276, 1454, 1501
Hin6I GCGC 1 cut(s) 1752
HinP1I GCGC 1 cut(s) 1752
HincII GTYRAC 2 cut(s) 415, 1278
HindII GTYRAC 2 cut(s) 415, 1278
HindIII AAGCTT 2 cut(s) 1016, 1240
HinfI GANTC 7 cut(s) 133, 177, 239, 642, 1070, 1397, 1601
HpaI GTTAAC 1 cut(s) 1278
HpaII CCGG 3 cut(s) 219, 253, 1760
HphI GGTGA 8 cut(s) 24, 151, 172, 248, 341, 589, 1933, 1965
Hpy166II GTNNAC 5 cut(s) 415, 1219, 1278, 1447, 1941
Hpy188I TCNGA 4 cut(s) 176, 797, 1559, 1689
Hpy188III TCNNGA 9 cut(s) 143, 194, 518, 713, 917, 929, 1098, 1464, 1760
Hpy8I GTNNAC 5 cut(s) 415, 1219, 1278, 1447, 1941
Hpy99I CGWCG 3 cut(s) 167, 766, 769
HpyAV CCTTC 6 cut(s) 62, 241, 331, 1019, 1114, 1519
HpyCH4III ACNGT 3 cut(s) 335, 1353, 1938
HpyCH4IV ACGT 3 cut(s) 767, 780, 1880
HpyF10VI GCNNNNNNNGC 2 cut(s) 402, 668
HpyF3I CTNAG 2 cut(s) 382, 1157
HpySE526I ACGT 3 cut(s) 767, 780, 1880
Hsp92I GRCGYC 1 cut(s) 767
Hsp92II CATG 7 cut(s) 533, 693, 920, 1231, 1276, 1454, 1501
HspAI GCGC 1 cut(s) 1752
Kpn2I TCCGGA 1 cut(s) 1759
Ksp22I TGATCA 2 cut(s) 579, 1684
KspAI GTTAAC 1 cut(s) 1278
Kzo9I GATC 7 cut(s) 514, 526, 536, 579, 604, 681, 1684
LmnI GCTCC 5 cut(s) 173, 213, 358, 368, 1648
Lsp1109I GCAGC 6 cut(s) 411, 649, 1040, 1142, 1347, 1691
LweI GCATC 4 cut(s) 142, 1065, 1171, 1833
MabI ACCWGGT 1 cut(s) 440
MaeI CTAG 6 cut(s) 143, 269, 350, 788, 1172, 1530
MaeII ACGT 3 cut(s) 767, 780, 1880
MaeIII GTNAC 4 cut(s) 329, 560, 817, 1513
MalI GATC 7 cut(s) 516, 528, 538, 581, 606, 683, 1686
MbiI CCGCTC 1 cut(s) 40
MboI GATC 7 cut(s) 514, 526, 536, 579, 604, 681, 1684
MfeI CAATTG 1 cut(s) 1888
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 3 cut(s) 193, 218, 1653
MlyI GAGTC 4 cut(s) 127, 171, 1064, 1391
MmeI TCCRAC 5 cut(s) 199, 619, 775, 795, 1374
Mph1103I ATGCAT 1 cut(s) 691
MroI TCCGGA 1 cut(s) 1759
MroXI GAANNNNTTC 1 cut(s) 1794
MseI TTAA 5 cut(s) 74, 303, 1086, 1277, 1806
MslI CAYNNNNRTG 1 cut(s) 1502
MspA1I CMGCKG 2 cut(s) 881, 1682
MspI CCGG 3 cut(s) 219, 253, 1760
MspR9I CCNGG 4 cut(s) 130, 377, 442, 958
MunI CAATTG 1 cut(s) 1888
MvaI CCWGG 4 cut(s) 130, 377, 442, 958
MwoI GCNNNNNNNGC 2 cut(s) 402, 668
NcoI CCATGG 1 cut(s) 1450
NdeI CATATG 1 cut(s) 1075
NdeII GATC 7 cut(s) 514, 526, 536, 579, 604, 681, 1684
NheI GCTAGC 1 cut(s) 1529
NlaIII CATG 7 cut(s) 533, 693, 920, 1231, 1276, 1454, 1501
NlaIV GGNNCC 5 cut(s) 169, 364, 927, 955, 1193
NmeAIII GCCGAG 1 cut(s) 1064
NmuCI GTSAC 1 cut(s) 329
NsiI ATGCAT 1 cut(s) 691
NspI RCATGY 2 cut(s) 1231, 1276
NspV TTCGAA 2 cut(s) 939, 1812
PagI TCATGA 1 cut(s) 916
PceI AGGCCT 2 cut(s) 380, 1777
PdmI GAANNNNTTC 1 cut(s) 1794
PfeI GAWTC 3 cut(s) 239, 642, 1601
PflFI GACNNNGTC 1 cut(s) 766
PinAI ACCGGT 1 cut(s) 218
PkrI GCNGC 8 cut(s) 42, 426, 664, 1055, 1132, 1318, 1362, 1681
PleI GAGTC 4 cut(s) 127, 171, 1064, 1391
PpsI GAGTC 4 cut(s) 127, 171, 1064, 1391
Psp124BI GAGCTC 1 cut(s) 1653
Psp1406I AACGTT 1 cut(s) 1880
Psp6I CCWGG 4 cut(s) 128, 375, 440, 956
PspFI CCCAGC 1 cut(s) 881
PspGI CCWGG 4 cut(s) 128, 375, 440, 956
PspN4I GGNNCC 5 cut(s) 169, 364, 927, 955, 1193
PspPI GGNCC 2 cut(s) 953, 982
PstI CTGCAG 1 cut(s) 667
PstNI CAGNNNCTG 2 cut(s) 662, 1693
PsuI RGATCY 1 cut(s) 514
PsyI GACNNNGTC 1 cut(s) 766
PvuII CAGCTG 2 cut(s) 881, 1682
RsaI GTAC 5 cut(s) 446, 573, 851, 892, 1295
RsaNI GTAC 5 cut(s) 445, 572, 850, 891, 1294
RseI CAYNNNNRTG 1 cut(s) 1502
SacI GAGCTC 1 cut(s) 1653
SaqAI TTAA 5 cut(s) 74, 303, 1086, 1277, 1806
SatI GCNGC 8 cut(s) 41, 425, 663, 1054, 1131, 1317, 1361, 1680
Sau3AI GATC 7 cut(s) 514, 526, 536, 579, 604, 681, 1684
Sau96I GGNCC 2 cut(s) 953, 982
ScaI AGTACT 1 cut(s) 892
SchI GAGTC 4 cut(s) 127, 171, 1064, 1391
ScrFI CCNGG 4 cut(s) 130, 377, 442, 958
SduI GDGCHC 3 cut(s) 193, 218, 1653
SexAI ACCWGGT 1 cut(s) 440
SfaNI GCATC 4 cut(s) 142, 1065, 1171, 1833
SfcI CTRYAG 2 cut(s) 663, 1549
SfuI TTCGAA 2 cut(s) 939, 1812
SmiMI CAYNNNNRTG 1 cut(s) 1502
SseBI AGGCCT 2 cut(s) 380, 1777
SsiI CCGC 4 cut(s) 40, 120, 327, 1317
SspMI CTAG 6 cut(s) 143, 269, 350, 788, 1172, 1530
SstI GAGCTC 1 cut(s) 1653
StuI AGGCCT 2 cut(s) 380, 1777
StyD4I CCNGG 4 cut(s) 128, 375, 440, 956
StyI CCWWGG 2 cut(s) 822, 1450
TaaI ACNGT 3 cut(s) 335, 1353, 1938
TaiI ACGT 3 cut(s) 770, 783, 1883
TatI WGTACW 2 cut(s) 571, 890
TauI GCSGC 2 cut(s) 43, 1319
TfiI GAWTC 3 cut(s) 239, 642, 1601
Tru1I TTAA 5 cut(s) 74, 303, 1086, 1277, 1806
Tru9I TTAA 5 cut(s) 74, 303, 1086, 1277, 1806
TscAI CASTG 3 cut(s) 215, 1358, 1941
TseFI GTSAC 1 cut(s) 329
TseI GCWGC 6 cut(s) 424, 662, 1053, 1130, 1360, 1679
Tsp45I GTSAC 1 cut(s) 329
TspDTI ATGAA 6 cut(s) 276, 280, 905, 948, 990, 1626
TspGWI ACGGA 1 cut(s) 1251
TspRI CASTG 3 cut(s) 215, 1358, 1941
Tth111I GACNNNGTC 1 cut(s) 766
XapI RAATTY 4 cut(s) 588, 694, 935, 1402
XbaI TCTAGA 1 cut(s) 142
XceI RCATGY 2 cut(s) 1231, 1276
XmiI GTMKAC 2 cut(s) 1218, 1446
XmnI GAANNNNTTC 1 cut(s) 1794
XspI CTAG 6 cut(s) 143, 269, 350, 788, 1172, 1530
ZraI GACGTC 1 cut(s) 768
ZrmI AGTACT 1 cut(s) 892
Zsp2I ATGCAT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.