MD05G1327900.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000112
Physical Location & Seq
Forward (+)
45341524 .. 45344921
3398 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1327900.v1.1.491

Sequence Viewer

Length: 2349 bp
ATGTTACTGAAAGCTTTGCTTGTTGTGTCCCTCCTCTTCCCATTTTGCACATCCATTGACACCATAGAAATGGACCAACATGTAAAGGATGGTGATCTTCTGGTGTCCAAAGGAAACATCTTCGCATTAGGGTTCTTCAGCCCCAGAAACTCCAGCTCCAGGTACGTCGGAATTTGGTATGTTCAAAACGATCAAAAAGGTCAAAAGGTAGTGGTTTGGGTAGCAAACAGAAACGACCCCATCAATCATACCTCCGGTGTCCTTACTATAGACGGGTATGGGAGACTGCTTTTTTATTCTAACAATATGCAGAACATTCCTGTGTGGTCTACAAATGTGACGGTTCAAACTACGAGCACTAGTTGCAGGGCTCAGCTTTTAGATTCTGGAAATTTAGTTCTTTTCCGGGATAATAAAAGCAAAAATTTTATGTGGCAGAGTTTTGATTATCCTACAGATACTCTCCTTCCGGGTATGAAACTAGGCGTGAATTGGAAATTGGGGATAGAATGGGTCTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGAATACACCACTAGGCTCTATTCCAATCAGACAGCCACCCCCGAATTTTTTACTTTTTATAAAGGTTTGACTCCGCATTGGCGATCTGATCCAGCGAACACGCGTACTATTGTCAATAATCAGGGCGAAACGTATTCCTTTGTTAAAAACGATAGCACTCCAACAAGGGCGGTGTTGAAGGATTCTGGGTTGCTGCAAATCCTCAGGTGGAACAAGGCAGATAGAGAATGGAAGGAACTATGGTCTGCGCCAAAATATCAGTGTGACCAGTATGAAGAGTGCGGTGCCAACAGCAAATGTAGTCCTGATAATATCAATTCGTTTGAGTGTGAGTGTCTGCCAGGGTATGAACCGAAGTCTGTCAATGATTGGAAGATGAGAGACGGTTCGGACGGGTGTGTGAGTAAACGAGTTGCAGTATCAAAGTGTAGGAACGGAGAAGGTTTCATGAAAGTGGCACGAGTTAAAGATCCAGACACAACGAAAGCAGCACGGCTGGAAAAAGGTATTAGTGTCAAAGAGTGTAAGCAGGTGTGCTTAAGCGATTGTTCTTGCACTGCATATATGATCATAGAATCTGAAGGGCGCAGTGATTGCTTGACATGGTATGGTAAGTTGCGGGACATTTTAGTGTACACACAGGCAGGGCGAGATCTGCATGTACGAGTGGACAAAATCGAATTAGCTGAAAATTTCAGAAATTCGAAAGGTTTTCTTAAAAGGAGGGGCATGCTGGCTATTCTGATGTTGTCTGTTCTTCTAGCATTGGTACTGATCGTTGCGTTGGCCTGTTGGAGGATTAGGAAAAAGAGGAAGACAAAAGTTGAGATTGAAGAAACTAGGAGACATCCCGAATTGCAGTTTTTCCATCTGAGAACGATAATTGCAGCTACGAACAACTTCTCTCCCGTCCAAAAACTTGGCCAAGGTGGTTTTGGCACTGTTTATAAGGGTGTGTTACGAAATAATCAGAATATTGCTGTAAAAAGATTATCCAGAGCTTCAGGACAAGGAGTTGAAGAATTCAAAAATGAAGTTGCGCTGATAGCGAGACTTCAACACAGGAATCTCGTGAAACTTTTAGGCTGTTGCATAAAGGGAGAAGAAAGGATGTTAGTCCTGGAATACTTGCCGAACAAAAGCTTGGACTACTTTCTTTTCGATCACACAAAAAAGTCCTTGCTGGATTGGCAAAAGCGATTCGAAATCATCAATGGGGTTGCTCGTGGGGTTTTGTATCTTCACCAAGACTCAAGACTTAGGATTATTCACAGGGATCTAAAAACCAGCAATGTCCTTCTAGACGCTGAGATGAACCCAAAAATATCAGATTTTGGCATGGCAAGAATCTTCCATGGGGACCAACTCCAAGATACGACCAACAGAGTTGTCGGAACATATGGCTATATGTCACCGGAGTATGCAGTGTTCGGGAGATTTTCAACCAAATCGGACGTTTTTAGTTTTGGGGTCATATTATTGGAGATTGTTTGGCAGTTGTGGAGTGAAGACAGAGCCTTAGAAATTGTGGATTCATCATTGGAGTCATACGCGCCCGACGAAGCCATGAGATGCATACAAGTCGGGCTCTTGTGTGTCGAAGAAGAGTCGAAGAACAGACCATCCATGTCAGCTGTTGTTTTTATGTTGAGCGGCGAAGCATCTGCTCCATCTCCTCAGCAGCCTGCATTTTCCTTGAGAAAAAATTCATGCGGCGATGCTGTTGTTCCGTCAGTTTCTGAAGGATCGTGTTCTATCAACGACGTGACAATAACTAAATTCGAAGGTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

783

Amino Acids

88.56

Weight (kDa)

8.78

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 71 - 177 1.1e-34 D-mannose binding lectin
S_locus_glycop PF00954 222 - 305 1e-18 S-locus glycoprotein domain
PAN_2 PF08276 328 - 394 2.9e-16 PAN-like domain
Pkinase PF00069 488 - 683 7.8e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 490 - 732 3.7e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 615, 1503
AarI CACCTGC 1 cut(s) 1075
Acc36I ACCTGC 1 cut(s) 1075
AccB1I GGYRCC 1 cut(s) 839
AccBSI CCGCTC 1 cut(s) 2208
AccI GTMKAC 1 cut(s) 329
AccII CGCG 2 cut(s) 658, 2108
AciI CCGC 6 cut(s) 629, 725, 837, 1174, 2208, 2268
AclWI GGATC 4 cut(s) 638, 1019, 1839, 2308
AcoI YGGCCR 1 cut(s) 1477
AcsI RAATTY 9 cut(s) 171, 391, 424, 599, 1246, 1255, 1577, 2260, 2333
AcuI CTGAAG 4 cut(s) 121, 1155, 1542, 2316
AfaI GTAC 5 cut(s) 164, 661, 1190, 1218, 1326
AfiI CCNNNNNNNGG 3 cut(s) 159, 551, 1350
AflII CTTAAG 1 cut(s) 1093
AflIII ACRYGT 2 cut(s) 79, 656
AgsI TTSAA 8 cut(s) 185, 347, 733, 1388, 1574, 1582, 1613, 1998
AhlI ACTAGT 1 cut(s) 359
AjiI CACGTC 1 cut(s) 2320
AjnI CCWGG 4 cut(s) 158, 544, 895, 1674
AjuI GAANNNNNNNTTGG 4 cut(s) 482, 514, 1682, 1714
AloI GAACNNNNNNTCC 4 cut(s) 381, 413, 2162, 2194
AluBI AGCT 8 cut(s) 14, 156, 376, 1241, 1445, 1556, 1698, 2189
AluI AGCT 8 cut(s) 14, 156, 376, 1241, 1445, 1556, 1698, 2189
Alw21I GWGCWC 1 cut(s) 359
Alw26I GTCTC 4 cut(s) 277, 930, 1393, 1600
AlwI GGATC 4 cut(s) 638, 1019, 1839, 2308
AlwNI CAGNNNCTG 1 cut(s) 2294
AoxI GGCC 2 cut(s) 1341, 1477
ApeKI GCWGC 4 cut(s) 748, 1043, 1442, 2236
ApoI RAATTY 9 cut(s) 171, 391, 424, 599, 1246, 1255, 1577, 2260, 2333
Asp700I GAANNNNTTC 2 cut(s) 1454, 2260
AspLEI GCGC 4 cut(s) 805, 1143, 1597, 2110
AspS9I GGNCC 2 cut(s) 73, 1915
AsuC2I CCSGG 2 cut(s) 407, 471
AsuHPI GGTGA 3 cut(s) 104, 1790, 1959
AsuII TTCGAA 3 cut(s) 1259, 1758, 2337
AvaII GGWCC 2 cut(s) 73, 1915
AxyI CCTNAGG 1 cut(s) 758
BalI TGGCCA 1 cut(s) 1479
BanI GGYRCC 1 cut(s) 839
BanII GRGCYC 2 cut(s) 373, 2145
BauI CACGAG 3 cut(s) 1014, 1625, 1779
BbsI GAAGAC 2 cut(s) 1376, 2070
Bbv12I GWGCWC 1 cut(s) 359
BbvCI CCTCAGC 1 cut(s) 2232
BbvI GCAGC 4 cut(s) 735, 1055, 1454, 2248
BccI CCATC 5 cut(s) 83, 248, 1431, 2185, 2233
BceAI ACGGC 1 cut(s) 1064
BcgI CGANNNNNNTGC 4 cut(s) 2119, 2153, 2201, 2235
BciT130I CCWGG 4 cut(s) 160, 546, 897, 1676
BclI TGATCA 1 cut(s) 1122
BcnI CCSGG 2 cut(s) 407, 471
BcoDI GTCTC 4 cut(s) 277, 930, 1393, 1600
BcuI ACTAGT 1 cut(s) 359
BfaI CTAG 6 cut(s) 360, 482, 567, 1316, 1395, 1856
BfmI CTRYAG 2 cut(s) 267, 453
BfrI CTTAAG 1 cut(s) 1093
BfuAI ACCTGC 1 cut(s) 1075
BglII AGATCT 1 cut(s) 1207
BisI GCNGC 6 cut(s) 749, 1044, 1443, 2209, 2237, 2269
BlpI GCTNAGC 1 cut(s) 372
BlsI GCNGC 6 cut(s) 750, 1045, 1444, 2210, 2238, 2270
Bme1390I CCNGG 6 cut(s) 160, 407, 471, 546, 897, 1676
Bme18I GGWCC 2 cut(s) 73, 1915
BmgBI CACGTC 1 cut(s) 2320
BmgT120I GGNCC 2 cut(s) 73, 1915
BmiI GGNNCC 2 cut(s) 841, 1916
BmrFI CCNGG 6 cut(s) 160, 407, 471, 546, 897, 1676
BmrI ACTGGG 1 cut(s) 561
BmsI GCATC 3 cut(s) 2117, 2225, 2263
BmuI ACTGGG 1 cut(s) 561
BpiI GAAGAC 2 cut(s) 1376, 2070
BpmI CTGGAG 2 cut(s) 136, 142
Bpu10I CCTNAGC 1 cut(s) 2232
Bpu1102I GCTNAGC 1 cut(s) 372
Bpu14I TTCGAA 3 cut(s) 1259, 1758, 2337
BpuEI CTTGAG 2 cut(s) 1792, 2272
BpuMI CCSGG 2 cut(s) 407, 471
BsaBI GATNNNNATC 1 cut(s) 93
BsaJI CCNNGG 4 cut(s) 544, 896, 1480, 1909
BsaWI WCCGGW 2 cut(s) 254, 1969
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 551, 1350
Bse1I ACTGG 2 cut(s) 556, 823
Bse21I CCTNAGG 1 cut(s) 758
Bse3DI GCAATG 1 cut(s) 1852
Bse8I GATNNNNATC 1 cut(s) 93
BseBI CCWGG 4 cut(s) 160, 546, 897, 1676
BseDI CCNNGG 4 cut(s) 544, 896, 1480, 1909
BseGI GGATG 5 cut(s) 50, 94, 1402, 1671, 2177
BseJI GATNNNNATC 1 cut(s) 93
BseLI CCNNNNNNNGG 3 cut(s) 159, 551, 1350
BseMI GCAATG 1 cut(s) 1852
BseMII CTCAG 5 cut(s) 386, 772, 1418, 1854, 2246
BseNI ACTGG 2 cut(s) 556, 823
BseRI GAGGAG 2 cut(s) 23, 2220
BseXI GCAGC 4 cut(s) 735, 1055, 1454, 2248
Bsh1236I CGCG 2 cut(s) 658, 2108
BshFI GGCC 2 cut(s) 1343, 1479
BshNI GGYRCC 1 cut(s) 839
BsiHKAI GWGCWC 1 cut(s) 359
BsiSI CCGG 4 cut(s) 255, 406, 470, 1970
BslFI GGGAC 3 cut(s) 13, 1190, 1928
BslI CCNNNNNNNGG 3 cut(s) 159, 551, 1350
BsmAI GTCTC 4 cut(s) 277, 930, 1393, 1600
BsmBI CGTCTC 1 cut(s) 930
BsmFI GGGAC 3 cut(s) 13, 1190, 1928
BsnI GGCC 2 cut(s) 1343, 1479
Bsp119I TTCGAA 3 cut(s) 1259, 1758, 2337
Bsp1286I GDGCHC 3 cut(s) 359, 373, 2145
Bsp1407I TGTACA 1 cut(s) 1188
Bsp1720I GCTNAGC 1 cut(s) 372
Bsp19I CCATGG 1 cut(s) 1909
BspACI CCGC 6 cut(s) 629, 725, 837, 1174, 2208, 2268
BspANI GGCC 2 cut(s) 1343, 1479
BspCNI CTCAG 5 cut(s) 385, 771, 1419, 1855, 2245
BspFNI CGCG 2 cut(s) 658, 2108
BspHI TCATGA 1 cut(s) 1002
BspLI GGNNCC 2 cut(s) 841, 1916
BspMI ACCTGC 1 cut(s) 1075
BspPI GGATC 4 cut(s) 638, 1019, 1839, 2308
BspT104I TTCGAA 3 cut(s) 1259, 1758, 2337
BspT107I GGYRCC 1 cut(s) 839
BspTI CTTAAG 1 cut(s) 1093
BsrBI CCGCTC 1 cut(s) 2208
BsrDI GCAATG 1 cut(s) 1852
BsrGI TGTACA 1 cut(s) 1188
BsrI ACTGG 2 cut(s) 556, 823
BssECI CCNNGG 4 cut(s) 544, 896, 1480, 1909
BssSI CACGAG 3 cut(s) 1014, 1625, 1779
BssT1I CCWWGG 2 cut(s) 1480, 1909
Bst2BI CACGAG 3 cut(s) 1014, 1625, 1779
Bst2UI CCWGG 4 cut(s) 160, 546, 897, 1676
Bst4CI ACNGT 3 cut(s) 343, 941, 1498
Bst6I CTCTTC 3 cut(s) 41, 825, 2154
BstAFI CTTAAG 1 cut(s) 1093
BstAPI GCANNNNNTGC 2 cut(s) 363, 1149
BstAUI TGTACA 1 cut(s) 1188
BstBI TTCGAA 3 cut(s) 1259, 1758, 2337
BstC8I GCNNGC 3 cut(s) 1286, 1290, 2241
BstDEI CTNAG 7 cut(s) 372, 758, 1427, 1814, 1863, 2074, 2232
BstDSI CCRYGG 1 cut(s) 1909
BstENI CCTNNNNNAGG 1 cut(s) 1348
BstF5I GGATG 5 cut(s) 50, 94, 1402, 1671, 2177
BstFNI CGCG 2 cut(s) 658, 2108
BstHHI GCGC 4 cut(s) 805, 1143, 1597, 2110
BstMAI GTCTC 4 cut(s) 277, 930, 1393, 1600
BstMWI GCNNNNNNNGC 5 cut(s) 363, 1149, 1210, 1601, 1744
BstNI CCWGG 4 cut(s) 160, 546, 897, 1676
BstNSI RCATGY 3 cut(s) 83, 1217, 1288
BstSCI CCNGG 6 cut(s) 158, 405, 469, 544, 895, 1674
BstSFI CTRYAG 2 cut(s) 267, 453
BstUI CGCG 2 cut(s) 658, 2108
BstV1I GCAGC 4 cut(s) 735, 1055, 1454, 2248
BstV2I GAAGAC 2 cut(s) 1376, 2070
BstX2I RGATCY 3 cut(s) 1024, 1207, 1831
BstXI CCANNNNNNTGG 2 cut(s) 70, 1475
BstYI RGATCY 3 cut(s) 1024, 1207, 1831
Bsu36I CCTNAGG 1 cut(s) 758
BsuRI GGCC 2 cut(s) 1343, 1479
BtgI CCRYGG 1 cut(s) 1909
BtgZI GCGATG 1 cut(s) 2286
BtrI CACGTC 1 cut(s) 2320
BtsCI GGATG 5 cut(s) 50, 94, 1402, 1671, 2177
BtsI GCAGTG 3 cut(s) 1110, 1150, 1986
BtsIMutI CAGTG 5 cut(s) 821, 1110, 1150, 1494, 1986
BveI ACCTGC 1 cut(s) 1075
Cac8I GCNNGC 3 cut(s) 1286, 1290, 2241
CaiI CAGNNNCTG 1 cut(s) 2294
CciI TCATGA 1 cut(s) 1002
CfoI GCGC 4 cut(s) 805, 1143, 1597, 2110
Cfr13I GGNCC 2 cut(s) 73, 1915
CseI GACGC 1 cut(s) 1868
Csp6I GTAC 5 cut(s) 163, 660, 1189, 1217, 1325
CviQI GTAC 5 cut(s) 163, 660, 1189, 1217, 1325
DdeI CTNAG 7 cut(s) 372, 758, 1427, 1814, 1863, 2074, 2232
EaeI YGGCCR 1 cut(s) 1477
Eam1104I CTCTTC 3 cut(s) 41, 825, 2154
EarI CTCTTC 3 cut(s) 41, 825, 2154
Eco130I CCWWGG 2 cut(s) 1480, 1909
Eco24I GRGCYC 2 cut(s) 373, 2145
Eco47I GGWCC 2 cut(s) 73, 1915
Eco57I CTGAAG 4 cut(s) 121, 1155, 1542, 2316
Eco81I CCTNAGG 1 cut(s) 758
EcoNI CCTNNNNNAGG 1 cut(s) 1348
EcoRI GAATTC 1 cut(s) 1577
EcoRII CCWGG 4 cut(s) 158, 544, 895, 1674
EcoT14I CCWWGG 2 cut(s) 1480, 1909
EcoT22I ATGCAT 1 cut(s) 2132
EcoT38I GRGCYC 2 cut(s) 373, 2145
ErhI CCWWGG 2 cut(s) 1480, 1909
Esp3I CGTCTC 1 cut(s) 930
FalI AAGNNNNNCTT 3 cut(s) 35, 1254, 1286
FaqI GGGAC 3 cut(s) 13, 1190, 1928
FauI CCCGC 1 cut(s) 1167
FauNDI CATATG 1 cut(s) 1954
FbaI TGATCA 1 cut(s) 1122
FblI GTMKAC 1 cut(s) 329
Fnu4HI GCNGC 6 cut(s) 749, 1044, 1443, 2209, 2237, 2269
FokI GGATG 5 cut(s) 37, 101, 1389, 1678, 2164
FriOI GRGCYC 2 cut(s) 373, 2145
Fsp4HI GCNGC 6 cut(s) 749, 1044, 1443, 2209, 2237, 2269
FspBI CTAG 6 cut(s) 360, 482, 567, 1316, 1395, 1856
GlaI GCGC 4 cut(s) 804, 1142, 1596, 2109
GluI GCNGC 6 cut(s) 749, 1044, 1443, 2209, 2237, 2269
GsuI CTGGAG 2 cut(s) 136, 142
HaeIII GGCC 2 cut(s) 1343, 1479
HapII CCGG 4 cut(s) 255, 406, 470, 1970
HgaI GACGC 1 cut(s) 1868
HhaI GCGC 4 cut(s) 805, 1143, 1597, 2110
Hin6I GCGC 4 cut(s) 803, 1141, 1595, 2108
HinP1I GCGC 4 cut(s) 803, 1141, 1595, 2108
HindIII AAGCTT 2 cut(s) 12, 1696
HpaII CCGG 4 cut(s) 255, 406, 470, 1970
HphI GGTGA 3 cut(s) 104, 1790, 1959
Hpy166II GTNNAC 5 cut(s) 330, 962, 1189, 1191, 1225
Hpy8I GTNNAC 5 cut(s) 330, 962, 1189, 1191, 1225
Hpy99I CGWCG 3 cut(s) 170, 2117, 2321
HpyAV CCTTC 8 cut(s) 476, 727, 781, 989, 1130, 1862, 2291, 2333
HpyCH4III ACNGT 3 cut(s) 343, 941, 1498
HpyCH4IV ACGT 4 cut(s) 165, 686, 2010, 2319
HpyF10VI GCNNNNNNNGC 5 cut(s) 363, 1149, 1210, 1601, 1744
HpyF3I CTNAG 7 cut(s) 372, 758, 1427, 1814, 1863, 2074, 2232
HpySE526I ACGT 4 cut(s) 165, 686, 2010, 2319
HspAI GCGC 4 cut(s) 803, 1141, 1595, 2108
Ksp22I TGATCA 1 cut(s) 1122
LmnI GCTCC 2 cut(s) 161, 2227
Lsp1109I GCAGC 4 cut(s) 735, 1055, 1454, 2248
LweI GCATC 3 cut(s) 2117, 2225, 2263
MaeI CTAG 6 cut(s) 360, 482, 567, 1316, 1395, 1856
MaeII ACGT 4 cut(s) 165, 686, 2010, 2319
MaeIII GTNAC 7 cut(s) 3, 337, 531, 818, 1512, 1965, 2320
MbiI CCGCTC 1 cut(s) 2208
MflI RGATCY 3 cut(s) 1024, 1207, 1831
MhlI GDGCHC 3 cut(s) 359, 373, 2145
MlsI TGGCCA 1 cut(s) 1479
MluI ACGCGT 1 cut(s) 656
MluNI TGGCCA 1 cut(s) 1479
MlyI GAGTC 4 cut(s) 619, 1799, 2108, 2171
MmeI TCCRAC 4 cut(s) 148, 740, 1328, 1927
MnlI CCTC 8 cut(s) 41, 44, 262, 767, 1272, 1344, 1359, 2241
Mox20I TGGCCA 1 cut(s) 1479
Mph1103I ATGCAT 1 cut(s) 2132
MroXI GAANNNNTTC 2 cut(s) 1454, 2260
MscI TGGCCA 1 cut(s) 1479
MseI TTAA 5 cut(s) 518, 699, 1020, 1094, 1272
MslI CAYNNNNRTG 4 cut(s) 68, 320, 1007, 1184
Msp20I TGGCCA 1 cut(s) 1479
MspA1I CMGCKG 1 cut(s) 2189
MspCI CTTAAG 1 cut(s) 1093
MspI CCGG 4 cut(s) 255, 406, 470, 1970
MspR9I CCNGG 6 cut(s) 160, 407, 471, 546, 897, 1676
MvaI CCWGG 4 cut(s) 160, 546, 897, 1676
MvnI CGCG 2 cut(s) 658, 2108
MwoI GCNNNNNNNGC 5 cut(s) 363, 1149, 1210, 1601, 1744
NciI CCSGG 2 cut(s) 407, 471
NcoI CCATGG 1 cut(s) 1909
NdeI CATATG 1 cut(s) 1954
NlaIV GGNNCC 2 cut(s) 841, 1916
NmuCI GTSAC 5 cut(s) 337, 531, 818, 1965, 2320
NsiI ATGCAT 1 cut(s) 2132
NspI RCATGY 3 cut(s) 83, 1217, 1288
NspV TTCGAA 3 cut(s) 1259, 1758, 2337
PaeI GCATGC 1 cut(s) 1288
PagI TCATGA 1 cut(s) 1002
PaqCI CACCTGC 1 cut(s) 1075
PciI ACATGT 1 cut(s) 79
PcsI WCGNNNNNNNCGW 1 cut(s) 2112
PdmI GAANNNNTTC 2 cut(s) 1454, 2260
PfeI GAWTC 7 cut(s) 383, 737, 1130, 1621, 1755, 1902, 2087
PfoI TCCNGGA 2 cut(s) 405, 1674
PkrI GCNGC 6 cut(s) 750, 1045, 1444, 2210, 2238, 2270
PleI GAGTC 4 cut(s) 619, 1799, 2107, 2170
PpsI GAGTC 4 cut(s) 619, 1799, 2107, 2170
PscI ACATGT 1 cut(s) 79
PsiI TTATAA 2 cut(s) 615, 1503
Psp6I CCWGG 4 cut(s) 158, 544, 895, 1674
PspGI CCWGG 4 cut(s) 158, 544, 895, 1674
PspN4I GGNNCC 2 cut(s) 841, 1916
PspPI GGNCC 2 cut(s) 73, 1915
PsrI GAACNNNNNNTAC 2 cut(s) 1967, 1999
PstNI CAGNNNCTG 1 cut(s) 2294
PsuI RGATCY 3 cut(s) 1024, 1207, 1831
PvuII CAGCTG 1 cut(s) 2189
RsaI GTAC 5 cut(s) 164, 661, 1190, 1218, 1326
RsaNI GTAC 5 cut(s) 163, 660, 1189, 1217, 1325
RseI CAYNNNNRTG 4 cut(s) 68, 320, 1007, 1184
SaqAI TTAA 5 cut(s) 518, 699, 1020, 1094, 1272
SatI GCNGC 6 cut(s) 749, 1044, 1443, 2209, 2237, 2269
Sau96I GGNCC 2 cut(s) 73, 1915
SchI GAGTC 4 cut(s) 619, 1799, 2108, 2171
ScrFI CCNGG 6 cut(s) 160, 407, 471, 546, 897, 1676
SduI GDGCHC 3 cut(s) 359, 373, 2145
SfaNI GCATC 3 cut(s) 2117, 2225, 2263
SfcI CTRYAG 2 cut(s) 267, 453
SfuI TTCGAA 3 cut(s) 1259, 1758, 2337
SinI GGWCC 2 cut(s) 73, 1915
SmiMI CAYNNNNRTG 4 cut(s) 68, 320, 1007, 1184
SmlI CTYRAG 3 cut(s) 1093, 1807, 2251
SmoI CTYRAG 3 cut(s) 1093, 1807, 2251
SpeI ACTAGT 1 cut(s) 359
SphI GCATGC 1 cut(s) 1288
SsiI CCGC 6 cut(s) 629, 725, 837, 1174, 2208, 2268
SspI AATATT 1 cut(s) 1531
SspMI CTAG 6 cut(s) 360, 482, 567, 1316, 1395, 1856
StyD4I CCNGG 6 cut(s) 158, 405, 469, 544, 895, 1674
StyI CCWWGG 2 cut(s) 1480, 1909
TaaI ACNGT 3 cut(s) 343, 941, 1498
TaiI ACGT 4 cut(s) 168, 689, 2013, 2322
TaqI TCGA 8 cut(s) 1233, 1259, 1716, 1758, 2154, 2165, 2337, 2344
TatI WGTACW 1 cut(s) 1188
TauI GCSGC 2 cut(s) 2211, 2271
TfiI GAWTC 7 cut(s) 383, 737, 1130, 1621, 1755, 1902, 2087
Tru1I TTAA 5 cut(s) 518, 699, 1020, 1094, 1272
Tru9I TTAA 5 cut(s) 518, 699, 1020, 1094, 1272
TscAI CASTG 5 cut(s) 821, 1117, 1150, 1501, 1986
TseFI GTSAC 5 cut(s) 337, 531, 818, 1965, 2320
TseI GCWGC 4 cut(s) 748, 1043, 1442, 2236
Tsp45I GTSAC 5 cut(s) 337, 531, 818, 1965, 2320
TspDTI ATGAA 9 cut(s) 491, 843, 918, 991, 1019, 1602, 1883, 2079, 2253
TspGWI ACGGA 2 cut(s) 1005, 2274
TspRI CASTG 5 cut(s) 821, 1117, 1150, 1501, 1986
Vha464I CTTAAG 1 cut(s) 1093
VpaK11BI GGWCC 2 cut(s) 73, 1915
XagI CCTNNNNNAGG 1 cut(s) 1348
XapI RAATTY 9 cut(s) 171, 391, 424, 599, 1246, 1255, 1577, 2260, 2333
XbaI TCTAGA 1 cut(s) 1855
XceI RCATGY 3 cut(s) 83, 1217, 1288
XcmI CCANNNNNNNNNTGG 1 cut(s) 1487
XmiI GTMKAC 1 cut(s) 329
XmnI GAANNNNTTC 2 cut(s) 1454, 2260
XspI CTAG 6 cut(s) 360, 482, 567, 1316, 1395, 1856
Zsp2I ATGCAT 1 cut(s) 2132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.