RLG00000031296

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000112
Physical Location & Seq
Forward (+)
3826402 .. 3827700
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031296

Sequence Viewer

Length: 1299 bp
ATGTTGCCGAGTACTTTGCTTTTGTTCCTTCTCTTCCCATTTTGCAGATCCGTAGGCACCATAGGACCAAAACAGTATGTCAAGGACGGGGACACCATAGTATCTAAAGAAATAAAGTTTGAGTTAGGCTTCTTCAGCCCAGGAAACTCTAGTTACCGGTATGTTGGAATTTGGTATGCTAAAGTACCTCAAAAACCAGTGGTATGGGTGGCAAACAGAGAGAAGCCAATTACTGATTCTTCTGGTGTACTCAAAATAAACAGGAATGGAAGCCTGGTACTTTTTTCTGACAATAAGGAGAGCTTGGAAATATGGTCAACAAAGATATCAGGCAAAACCAATAGTAGTAGTACTTCAGTTCAGCTTTTGGATACAGGAAACTTGGTTTTGGTCCAAGGTGAAAATAGTGAAGATGTATTATGGCAAAGCTTTGATTATCCTACAGATACTATGCTTCCAGGAATGAAACTTGGGTTAAATCGGGAGGTCCAGCTGAACAGCTTCTTAACATCCTGGAAGTCACAAGATGACCCAGGAACCGGGGACTTTTCATATAGGCTAAACACTACTGGCTCTCCAGAATTCTTTTTGTACAATGGTTCAACTCAGTATTGGCGAAGTGGTTCATGGCCATGGCGAAATATATCAGTCTCCACAAGTCACTCAAATCCTTTCATTTTTGTCAATAATCAAGTTGAGACATACATAATCGATTCTGCTCATAACACCTCTGAACTTTCAAGATATGTGGTGAACTATCATGGGACACTTCAGCGCCTAACGTGGAATGATATTGAGAGTCAATGGAAGGAAATATGGGCTGCACCAAAGTACCCATGTGACCAATATGGAGAGTGTGGTGCCTTCAGTACATGTAGTCCTGACAATATTCAAGGTCTTAAGTGTACATGTTTGCCGGGCTATGAACCAAGGTCTTTTGAGGATTGGTATCAGGGAGATGGATCAGATGGTTGTGTGAGTAAGCAGCCACAAGTGTCGAAGTGTGAGAATGGAGGTTTCAAGGTGGTGCAAAATGTGAAGCTTCCAGATGCATCAATATCAGCTTGGTTGGACACAAAAATGAGTGCCGCAGCATGCAAGCGTGCATGCTTGAGGAATTGTTCATGCCTTGCGTATTCAAGCCTAGAAATTGATGGAGGAGGTGGTTGCTTGGCCTGGTATGGTGAATTGATGGACATTTTATTGTTTGCAGAGGAGGGAAGCGACTTCTATGTTCGAGTGGATTCATCTGAATTAGGTACATGTTTCCTATTCCAAGGCAGAAAACATGAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

433

Amino Acids

48.34

Weight (kDa)

5.16

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 67 - 173 1.9e-37 D-mannose binding lectin
S_locus_glycop PF00954 205 - 311 3e-19 S-locus glycoprotein domain
PAN_2 PF08276 335 - 400 1.5e-17 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 56, 860
AciI CCGC 1 cut(s) 1089
AclWI GGATC 2 cut(s) 42, 970
AcoI YGGCCR 1 cut(s) 629
AcsI RAATTY 2 cut(s) 168, 581
AcuI CTGAAG 4 cut(s) 118, 339, 755, 850
AfiI CCNNNNNNNGG 1 cut(s) 539
AflII CTTAAG 1 cut(s) 899
AflIII ACRYGT 3 cut(s) 872, 908, 1262
AgeI ACCGGT 1 cut(s) 156
AgsI TTSAA 5 cut(s) 603, 741, 893, 1021, 1140
AjnI CCWGG 6 cut(s) 139, 273, 457, 512, 532, 1175
AjuI GAANNNNNNNTTGG 2 cut(s) 220, 252
AluBI AGCT 7 cut(s) 303, 364, 429, 493, 501, 1042, 1064
AluI AGCT 7 cut(s) 303, 364, 429, 493, 501, 1042, 1064
Alw26I GTCTC 2 cut(s) 655, 692
AlwI GGATC 2 cut(s) 42, 970
AoxI GGCC 2 cut(s) 629, 1173
ApeKI GCWGC 3 cut(s) 821, 985, 1091
ApoI RAATTY 2 cut(s) 168, 581
AsiGI ACCGGT 1 cut(s) 156
Asp700I GAANNNNTTC 2 cut(s) 500, 622
AspLEI GCGC 1 cut(s) 777
AspS9I GGNCC 3 cut(s) 65, 391, 487
AsuC2I CCSGG 2 cut(s) 541, 918
AsuHPI GGTGA 3 cut(s) 410, 763, 1196
AvaII GGWCC 3 cut(s) 65, 391, 487
BaeI ACNNNNGTAYC 4 cut(s) 84, 117, 815, 848
BalI TGGCCA 1 cut(s) 631
BanI GGYRCC 2 cut(s) 56, 860
BarI GAAGNNNNNNTAC 2 cut(s) 337, 369
BbvI GCAGC 3 cut(s) 808, 997, 1103
BccI CCATC 4 cut(s) 953, 962, 1148, 1186
BcgI CGANNNNNNTGC 1 cut(s) 32
BciT130I CCWGG 6 cut(s) 141, 275, 459, 514, 534, 1177
BciVI GTATCC 1 cut(s) 364
BcnI CCSGG 2 cut(s) 541, 918
BcoDI GTCTC 2 cut(s) 655, 692
BfaI CTAG 2 cut(s) 150, 1145
BfmI CTRYAG 1 cut(s) 441
BfoI RGCGCY 1 cut(s) 778
BfrI CTTAAG 1 cut(s) 899
BfuI GTATCC 1 cut(s) 364
BisI GCNGC 4 cut(s) 822, 986, 1089, 1092
BlsI GCNGC 4 cut(s) 823, 987, 1090, 1093
BmcAI AGTACT 2 cut(s) 13, 352
Bme1390I CCNGG 8 cut(s) 141, 275, 459, 514, 534, 541, 918, 1177
Bme18I GGWCC 3 cut(s) 65, 391, 487
BmgT120I GGNCC 3 cut(s) 65, 391, 487
BmiI GGNNCC 3 cut(s) 58, 538, 862
BmrFI CCNGG 8 cut(s) 141, 275, 459, 514, 534, 541, 918, 1177
BmsI GCATC 2 cut(s) 1039, 1061
BpmI CTGGAG 1 cut(s) 561
BpuEI CTTGAG 1 cut(s) 1132
BpuMI CCSGG 2 cut(s) 541, 918
Bsa29I ATCGAT 1 cut(s) 711
BsaBI GATNNNNATC 1 cut(s) 948
BsaJI CCNNGG 7 cut(s) 139, 394, 532, 540, 632, 929, 1276
BsaWI WCCGGW 1 cut(s) 156
BsaXI ACNNNNNCTCC 2 cut(s) 559, 589
Bsc4I CCNNNNNNNGG 1 cut(s) 539
Bse118I RCCGGY 1 cut(s) 156
Bse1I ACTGG 2 cut(s) 197, 574
Bse8I GATNNNNATC 1 cut(s) 948
BseBI CCWGG 6 cut(s) 141, 275, 459, 514, 534, 1177
BseCI ATCGAT 1 cut(s) 711
BseDI CCNNGG 7 cut(s) 139, 394, 532, 540, 632, 929, 1276
BseGI GGATG 1 cut(s) 509
BseJI GATNNNNATC 1 cut(s) 948
BseLI CCNNNNNNNGG 1 cut(s) 539
BseMII CTCAG 1 cut(s) 620
BseNI ACTGG 2 cut(s) 197, 574
BseRI GAGGAG 2 cut(s) 1173, 1229
BseXI GCAGC 3 cut(s) 808, 997, 1103
BsgI GTGCAG 1 cut(s) 807
BshFI GGCC 2 cut(s) 631, 1175
BshNI GGYRCC 2 cut(s) 56, 860
BshTI ACCGGT 1 cut(s) 156
BshVI ATCGAT 1 cut(s) 711
BsiSI CCGG 3 cut(s) 157, 540, 917
BslFI GGGAC 3 cut(s) 104, 557, 778
BslI CCNNNNNNNGG 1 cut(s) 539
BsmAI GTCTC 2 cut(s) 655, 692
BsmFI GGGAC 3 cut(s) 104, 557, 778
BsnI GGCC 2 cut(s) 631, 1175
Bsp1407I TGTACA 2 cut(s) 591, 905
Bsp143I GATC 2 cut(s) 47, 962
Bsp19I CCATGG 1 cut(s) 632
BspACI CCGC 1 cut(s) 1089
BspANI GGCC 2 cut(s) 631, 1175
BspCNI CTCAG 1 cut(s) 619
BspDI ATCGAT 1 cut(s) 711
BspLI GGNNCC 3 cut(s) 58, 538, 862
BspPI GGATC 2 cut(s) 42, 970
BspT107I GGYRCC 2 cut(s) 56, 860
BspTI CTTAAG 1 cut(s) 899
BsrFI RCCGGY 1 cut(s) 156
BsrGI TGTACA 2 cut(s) 591, 905
BsrI ACTGG 2 cut(s) 197, 574
BssAI RCCGGY 1 cut(s) 156
BssECI CCNNGG 7 cut(s) 139, 394, 532, 540, 632, 929, 1276
BssMI GATC 2 cut(s) 47, 962
BssT1I CCWWGG 4 cut(s) 394, 632, 929, 1276
Bst2UI CCWGG 6 cut(s) 141, 275, 459, 514, 534, 1177
Bst4CI ACNGT 1 cut(s) 75
Bst6I CTCTTC 1 cut(s) 38
BstAFI CTTAAG 1 cut(s) 899
BstAUI TGTACA 2 cut(s) 591, 905
BstC8I GCNNGC 4 cut(s) 1096, 1100, 1104, 1108
BstDEI CTNAG 1 cut(s) 606
BstDSI CCRYGG 1 cut(s) 632
BstF5I GGATG 1 cut(s) 509
BstH2I RGCGCY 1 cut(s) 778
BstHHI GCGC 1 cut(s) 777
BstKTI GATC 2 cut(s) 50, 965
BstMAI GTCTC 2 cut(s) 655, 692
BstMBI GATC 2 cut(s) 47, 962
BstMWI GCNNNNNNNGC 1 cut(s) 135
BstNI CCWGG 6 cut(s) 141, 275, 459, 514, 534, 1177
BstNSI RCATGY 5 cut(s) 876, 912, 1098, 1110, 1266
BstSCI CCNGG 8 cut(s) 139, 273, 457, 512, 532, 539, 916, 1175
BstSFI CTRYAG 1 cut(s) 441
BstV1I GCAGC 3 cut(s) 808, 997, 1103
BstX2I RGATCY 1 cut(s) 47
BstXI CCANNNNNNTGG 1 cut(s) 204
BstYI RGATCY 1 cut(s) 47
Bsu15I ATCGAT 1 cut(s) 711
BsuI GTATCC 1 cut(s) 364
BsuRI GGCC 2 cut(s) 631, 1175
BsuTUI ATCGAT 1 cut(s) 711
BtgI CCRYGG 1 cut(s) 632
BtsCI GGATG 1 cut(s) 509
BtsIMutI CAGTG 1 cut(s) 204
Cac8I GCNNGC 4 cut(s) 1096, 1100, 1104, 1108
CfoI GCGC 1 cut(s) 777
Cfr10I RCCGGY 1 cut(s) 156
Cfr13I GGNCC 3 cut(s) 65, 391, 487
ClaI ATCGAT 1 cut(s) 711
CspAI ACCGGT 1 cut(s) 156
CspCI CAANNNNNGTGG 2 cut(s) 729, 764
DdeI CTNAG 1 cut(s) 606
DpnI GATC 2 cut(s) 49, 964
DpnII GATC 2 cut(s) 47, 962
EaeI YGGCCR 1 cut(s) 629
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco130I CCWWGG 4 cut(s) 394, 632, 929, 1276
Eco32I GATATC 1 cut(s) 327
Eco47I GGWCC 3 cut(s) 65, 391, 487
Eco57I CTGAAG 4 cut(s) 118, 339, 755, 850
EcoRI GAATTC 1 cut(s) 581
EcoRII CCWGG 6 cut(s) 139, 273, 457, 512, 532, 1175
EcoRV GATATC 1 cut(s) 327
EcoT14I CCWWGG 4 cut(s) 394, 632, 929, 1276
EcoT22I ATGCAT 1 cut(s) 1054
ErhI CCWWGG 4 cut(s) 394, 632, 929, 1276
FalI AAGNNNNNCTT 2 cut(s) 287, 319
FaqI GGGAC 3 cut(s) 104, 557, 778
Fnu4HI GCNGC 4 cut(s) 822, 986, 1089, 1092
FokI GGATG 1 cut(s) 496
Fsp4HI GCNGC 4 cut(s) 822, 986, 1089, 1092
FspBI CTAG 2 cut(s) 150, 1145
GlaI GCGC 1 cut(s) 776
GluI GCNGC 4 cut(s) 822, 986, 1089, 1092
GsuI CTGGAG 1 cut(s) 561
HaeII RGCGCY 1 cut(s) 778
HaeIII GGCC 2 cut(s) 631, 1175
HapII CCGG 3 cut(s) 157, 540, 917
HhaI GCGC 1 cut(s) 777
Hin6I GCGC 1 cut(s) 775
HinP1I GCGC 1 cut(s) 775
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HindIII AAGCTT 2 cut(s) 427, 1040
HinfI GANTC 4 cut(s) 236, 713, 799, 1244
HpaII CCGG 3 cut(s) 157, 540, 917
HphI GGTGA 3 cut(s) 410, 763, 1196
Hpy166II GTNNAC 4 cut(s) 248, 318, 754, 906
Hpy188I TCNGA 4 cut(s) 289, 733, 967, 1252
Hpy188III TCNNGA 5 cut(s) 482, 578, 741, 881, 1046
Hpy8I GTNNAC 4 cut(s) 248, 318, 754, 906
HpyAV CCTTC 3 cut(s) 38, 802, 874
HpyCH4III ACNGT 1 cut(s) 75
HpyCH4IV ACGT 1 cut(s) 782
HpyCH4V TGCA 7 cut(s) 45, 824, 1030, 1052, 1098, 1106, 1211
HpyF10VI GCNNNNNNNGC 1 cut(s) 135
HpyF3I CTNAG 1 cut(s) 606
HpySE526I ACGT 1 cut(s) 782
HspAI GCGC 1 cut(s) 775
Kzo9I GATC 2 cut(s) 47, 962
Lsp1109I GCAGC 3 cut(s) 808, 997, 1103
LweI GCATC 2 cut(s) 1039, 1061
MaeI CTAG 2 cut(s) 150, 1145
MaeII ACGT 1 cut(s) 782
MaeIII GTNAC 4 cut(s) 152, 519, 659, 839
MalI GATC 2 cut(s) 49, 964
MboI GATC 2 cut(s) 47, 962
MboII GAAGA 4 cut(s) 25, 124, 231, 422
MflI RGATCY 1 cut(s) 47
MlsI TGGCCA 1 cut(s) 631
MluCI AATT 7 cut(s) 168, 228, 581, 1117, 1149, 1187, 1253
MluNI TGGCCA 1 cut(s) 631
MlyI GAGTC 1 cut(s) 808
MmeI TCCRAC 2 cut(s) 145, 1050
Mox20I TGGCCA 1 cut(s) 631
Mph1103I ATGCAT 1 cut(s) 1054
MroXI GAANNNNTTC 2 cut(s) 500, 622
MscI TGGCCA 1 cut(s) 631
MseI TTAA 3 cut(s) 476, 506, 900
MslI CAYNNNNRTG 2 cut(s) 631, 1079
Msp20I TGGCCA 1 cut(s) 631
MspA1I CMGCKG 1 cut(s) 493
MspCI CTTAAG 1 cut(s) 899
MspI CCGG 3 cut(s) 157, 540, 917
MspR9I CCNGG 8 cut(s) 141, 275, 459, 514, 534, 541, 918, 1177
MvaI CCWGG 6 cut(s) 141, 275, 459, 514, 534, 1177
MwoI GCNNNNNNNGC 1 cut(s) 135
NciI CCSGG 2 cut(s) 541, 918
NcoI CCATGG 1 cut(s) 632
NdeII GATC 2 cut(s) 47, 962
NlaIV GGNNCC 3 cut(s) 58, 538, 862
NmeAIII GCCGAG 1 cut(s) 33
NmuCI GTSAC 3 cut(s) 519, 659, 839
NsiI ATGCAT 1 cut(s) 1054
NspI RCATGY 5 cut(s) 876, 912, 1098, 1110, 1266
PaeI GCATGC 2 cut(s) 1098, 1110
PciI ACATGT 3 cut(s) 872, 908, 1262
PdmI GAANNNNTTC 2 cut(s) 500, 622
PfeI GAWTC 3 cut(s) 236, 713, 1244
PfoI TCCNGGA 2 cut(s) 457, 512
PinAI ACCGGT 1 cut(s) 156
PkrI GCNGC 4 cut(s) 823, 987, 1090, 1093
PleI GAGTC 1 cut(s) 807
PpsI GAGTC 1 cut(s) 807
PscI ACATGT 3 cut(s) 872, 908, 1262
Psp6I CCWGG 6 cut(s) 139, 273, 457, 512, 532, 1175
PspGI CCWGG 6 cut(s) 139, 273, 457, 512, 532, 1175
PspN4I GGNNCC 3 cut(s) 58, 538, 862
PspPI GGNCC 3 cut(s) 65, 391, 487
PsuI RGATCY 1 cut(s) 47
PvuII CAGCTG 1 cut(s) 493
RseI CAYNNNNRTG 2 cut(s) 631, 1079
SaqAI TTAA 3 cut(s) 476, 506, 900
SatI GCNGC 4 cut(s) 822, 986, 1089, 1092
Sau3AI GATC 2 cut(s) 47, 962
Sau96I GGNCC 3 cut(s) 65, 391, 487
ScaI AGTACT 2 cut(s) 13, 352
SchI GAGTC 1 cut(s) 808
ScrFI CCNGG 8 cut(s) 141, 275, 459, 514, 534, 541, 918, 1177
SfaNI GCATC 2 cut(s) 1039, 1061
SfcI CTRYAG 1 cut(s) 441
SinI GGWCC 3 cut(s) 65, 391, 487
SmiMI CAYNNNNRTG 2 cut(s) 631, 1079
SmlI CTYRAG 2 cut(s) 899, 1111
SmoI CTYRAG 2 cut(s) 899, 1111
SphI GCATGC 2 cut(s) 1098, 1110
Sse9I AATT 7 cut(s) 168, 228, 581, 1117, 1149, 1187, 1253
SsiI CCGC 1 cut(s) 1089
SspI AATATT 1 cut(s) 889
SspMI CTAG 2 cut(s) 150, 1145
StyD4I CCNGG 8 cut(s) 139, 273, 457, 512, 532, 539, 916, 1175
StyI CCWWGG 4 cut(s) 394, 632, 929, 1276
TaaI ACNGT 1 cut(s) 75
TaiI ACGT 1 cut(s) 785
TaqI TCGA 3 cut(s) 711, 998, 1237
TasI AATT 7 cut(s) 168, 228, 581, 1117, 1149, 1187, 1253
TatI WGTACW 6 cut(s) 11, 247, 350, 591, 869, 905
TauI GCSGC 1 cut(s) 1091
TfiI GAWTC 3 cut(s) 236, 713, 1244
Tru1I TTAA 3 cut(s) 476, 506, 900
Tru9I TTAA 3 cut(s) 476, 506, 900
TscAI CASTG 1 cut(s) 204
TseFI GTSAC 3 cut(s) 519, 659, 839
TseI GCWGC 3 cut(s) 821, 985, 1091
Tsp45I GTSAC 3 cut(s) 519, 659, 839
TspDTI ATGAA 7 cut(s) 479, 540, 615, 664, 939, 1113, 1236
TspGWI ACGGA 1 cut(s) 40
TspRI CASTG 1 cut(s) 204
Vha464I CTTAAG 1 cut(s) 899
VpaK11BI GGWCC 3 cut(s) 65, 391, 487
XapI RAATTY 2 cut(s) 168, 581
XceI RCATGY 5 cut(s) 876, 912, 1098, 1110, 1266
XmnI GAANNNNTTC 2 cut(s) 500, 622
XspI CTAG 2 cut(s) 150, 1145
ZrmI AGTACT 2 cut(s) 13, 352
Zsp2I ATGCAT 1 cut(s) 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.