Rroxscaffold_1G00073080

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000112
Physical Location & Seq
Reverse (-)
94065429 .. 94068925
3497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00073080.1

Sequence Viewer

Length: 768 bp
ATGTTAGTCCTAGAATACATGCCTAACAAAAGCTTGGACTCCTTTCTTTTTGAGTATCATTTGCATTTTGATGTAGATAACACAAGACGGTCCTTCTTGGATTGGGAAAAGCGCTTTGAAATTATCAACGGGATTGCTCGTGGGATTCTATATCTTCACCAAGACTCAAGATTGAGGATTATCCATAGAGATCTAAAAACTAGCAATGTTCTACTAGATGCTGAGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATATTCCACAGGGACCAACTGCAAGATAAGACGAACCGAATTGTCGGAACATATGGCTACATGTCACCGGAGTACGCGGTATTTGGAAGATTTTCCACAAAATCTGATGTCTTTAGTTTTGGGATCATAATGTTGGAGATTTTAAGTGGCCAGAAAAACAATGGTTTTGATCTGGAGGATCCTTCCATGAACTTAATAAGATGTGTTTGGGAGCTCTGGGAAGAAGGCAGAGCCCTAGATATTGTGGATTCAACGCTGAAATCATATCAGCCTAATGAAGTCATGAGATGCATACAAGTTGCACTCTTGTGTGTACAAGAAGATTCGAAGGACCGACCTGCCATGTCAGCCATTGTTTTCATGTTGAGTGGTGAAGCATCTCCTCCATTGCCTAACCAGCCGGCATTTGTTTACAGAAGAAATTCCGGCACTGATGTTTATCCATTACATTCAAACAGATCTTATTCTATAAACGATGTGACAAGATCTACAATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

255

Amino Acids

29.55

Weight (kDa)

5.72

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 1 - 207 4.2e-32 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 2 - 205 9.2e-29 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 607
AccII CGCG 1 cut(s) 338
AciI CCGC 1 cut(s) 338
AclWI GGATC 3 cut(s) 392, 434, 447
AcoI YGGCCR 1 cut(s) 409
AcsI RAATTY 2 cut(s) 237, 682
AfaI GTAC 2 cut(s) 335, 576
AfeI AGCGCT 1 cut(s) 113
AflIII ACRYGT 1 cut(s) 321
AgsI TTSAA 3 cut(s) 119, 513, 714
AleI CACNNNNGTG 1 cut(s) 568
AluBI AGCT 3 cut(s) 33, 475, 761
AluI AGCT 3 cut(s) 33, 475, 761
Alw21I GWGCWC 1 cut(s) 477
AlwI GGATC 3 cut(s) 392, 434, 447
Aor51HI AGCGCT 1 cut(s) 113
AoxI GGCC 1 cut(s) 409
ApoI RAATTY 2 cut(s) 237, 682
Asp700I GAANNNNTTC 2 cut(s) 352, 682
AspLEI GCGC 1 cut(s) 114
AspS9I GGNCC 3 cut(s) 90, 274, 592
AsuHPI GGTGA 3 cut(s) 149, 318, 644
AsuII TTCGAA 1 cut(s) 587
AvaII GGWCC 3 cut(s) 90, 274, 592
BalI TGGCCA 1 cut(s) 411
BamHI GGATCC 1 cut(s) 439
BanII GRGCYC 2 cut(s) 477, 496
BauI CACGAG 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 477
BfaI CTAG 5 cut(s) 11, 201, 215, 258, 497
BfoI RGCGCY 1 cut(s) 115
BfuAI ACCTGC 1 cut(s) 607
BglII AGATCT 3 cut(s) 190, 719, 746
Bme18I GGWCC 3 cut(s) 90, 274, 592
BmgT120I GGNCC 3 cut(s) 90, 274, 592
BmiI GGNNCC 2 cut(s) 275, 441
BmsI GCATC 3 cut(s) 208, 539, 647
BpmI CTGGAG 1 cut(s) 455
Bpu14I TTCGAA 1 cut(s) 587
BpuEI CTTGAG 1 cut(s) 151
BsaBI GATNNNNATC 1 cut(s) 699
BsaWI WCCGGW 1 cut(s) 328
Bse118I RCCGGY 1 cut(s) 661
Bse3DI GCAATG 2 cut(s) 211, 647
Bse8I GATNNNNATC 1 cut(s) 699
BseJI GATNNNNATC 1 cut(s) 699
BseMI GCAATG 2 cut(s) 211, 647
BseMII CTCAG 1 cut(s) 213
BseRI GAGGAG 1 cut(s) 633
Bsh1236I CGCG 1 cut(s) 338
BshFI GGCC 1 cut(s) 411
BsiHKAI GWGCWC 1 cut(s) 477
BsiSI CCGG 3 cut(s) 329, 662, 687
BslFI GGGAC 1 cut(s) 287
BsmFI GGGAC 1 cut(s) 287
BsnI GGCC 1 cut(s) 411
Bsp119I TTCGAA 1 cut(s) 587
Bsp1286I GDGCHC 2 cut(s) 477, 496
Bsp1407I TGTACA 1 cut(s) 574
Bsp143I GATC 6 cut(s) 190, 384, 430, 439, 719, 746
BspACI CCGC 1 cut(s) 338
BspANI GGCC 1 cut(s) 411
BspCNI CTCAG 1 cut(s) 214
BspFNI CGCG 1 cut(s) 338
BspHI TCATGA 1 cut(s) 543
BspLI GGNNCC 2 cut(s) 275, 441
BspMI ACCTGC 1 cut(s) 607
BspPI GGATC 3 cut(s) 392, 434, 447
BspT104I TTCGAA 1 cut(s) 587
BsrDI GCAATG 2 cut(s) 211, 647
BsrFI RCCGGY 1 cut(s) 661
BsrGI TGTACA 1 cut(s) 574
BssAI RCCGGY 1 cut(s) 661
BssMI GATC 6 cut(s) 190, 384, 430, 439, 719, 746
BssSI CACGAG 1 cut(s) 138
Bst2BI CACGAG 1 cut(s) 138
Bst4CI ACNGT 1 cut(s) 90
BstAUI TGTACA 1 cut(s) 574
BstBI TTCGAA 1 cut(s) 587
BstC8I GCNNGC 1 cut(s) 663
BstDEI CTNAG 1 cut(s) 222
BstFNI CGCG 1 cut(s) 338
BstH2I RGCGCY 1 cut(s) 115
BstHHI GCGC 1 cut(s) 114
BstKTI GATC 6 cut(s) 193, 387, 433, 442, 722, 749
BstMBI GATC 6 cut(s) 190, 384, 430, 439, 719, 746
BstMWI GCNNNNNNNGC 2 cut(s) 608, 658
BstNSI RCATGY 2 cut(s) 22, 325
BstUI CGCG 1 cut(s) 338
BstX2I RGATCY 4 cut(s) 190, 439, 719, 746
BstYI RGATCY 4 cut(s) 190, 439, 719, 746
BsuRI GGCC 1 cut(s) 411
BtsIMutI CAGTG 1 cut(s) 690
BveI ACCTGC 1 cut(s) 607
Cac8I GCNNGC 1 cut(s) 663
CciI TCATGA 1 cut(s) 543
CfoI GCGC 1 cut(s) 114
Cfr10I RCCGGY 1 cut(s) 661
Cfr13I GGNCC 3 cut(s) 90, 274, 592
Csp6I GTAC 2 cut(s) 334, 575
CviAII CATG 7 cut(s) 19, 253, 322, 448, 544, 604, 622
CviQI GTAC 2 cut(s) 334, 575
DdeI CTNAG 1 cut(s) 222
DpnI GATC 6 cut(s) 192, 386, 432, 441, 721, 748
DpnII GATC 6 cut(s) 190, 384, 430, 439, 719, 746
EaeI YGGCCR 1 cut(s) 409
Ecl136II GAGCTC 1 cut(s) 475
Eco24I GRGCYC 2 cut(s) 477, 496
Eco47I GGWCC 3 cut(s) 90, 274, 592
Eco47III AGCGCT 1 cut(s) 113
Eco53kI GAGCTC 1 cut(s) 475
EcoICRI GAGCTC 1 cut(s) 475
EcoT22I ATGCAT 1 cut(s) 554
EcoT38I GRGCYC 2 cut(s) 477, 496
FaeI CATG 7 cut(s) 22, 256, 325, 451, 547, 607, 625
FaqI GGGAC 1 cut(s) 287
FatI CATG 7 cut(s) 18, 252, 321, 447, 543, 603, 621
FauNDI CATATG 1 cut(s) 313
FriOI GRGCYC 2 cut(s) 477, 496
FspBI CTAG 5 cut(s) 11, 201, 215, 258, 497
GlaI GCGC 1 cut(s) 113
GsuI CTGGAG 1 cut(s) 455
HaeII RGCGCY 1 cut(s) 115
HaeIII GGCC 1 cut(s) 411
HapII CCGG 3 cut(s) 329, 662, 687
HhaI GCGC 1 cut(s) 114
Hin1II CATG 7 cut(s) 22, 256, 325, 451, 547, 607, 625
Hin6I GCGC 1 cut(s) 112
HinP1I GCGC 1 cut(s) 112
HindIII AAGCTT 1 cut(s) 31
HinfI GANTC 5 cut(s) 38, 145, 164, 509, 584
HpaII CCGG 3 cut(s) 329, 662, 687
HphI GGTGA 3 cut(s) 149, 318, 644
Hpy166II GTNNAC 2 cut(s) 575, 673
Hpy188I TCNGA 3 cut(s) 244, 308, 367
Hpy188III TCNNGA 3 cut(s) 168, 434, 544
Hpy8I GTNNAC 2 cut(s) 575, 673
HpyAV CCTTC 4 cut(s) 103, 453, 479, 583
HpyCH4III ACNGT 1 cut(s) 90
HpyCH4V TGCA 4 cut(s) 64, 283, 552, 563
HpyF10VI GCNNNNNNNGC 2 cut(s) 608, 658
HpyF3I CTNAG 1 cut(s) 222
Hsp92II CATG 7 cut(s) 22, 256, 325, 451, 547, 607, 625
HspAI GCGC 1 cut(s) 112
KroI GCCGGC 1 cut(s) 661
KroNI GCCGGC 1 cut(s) 663
Kzo9I GATC 6 cut(s) 190, 384, 430, 439, 719, 746
LmnI GCTCC 1 cut(s) 472
LpnPI CCDG 9 cut(s) 256, 342, 419, 425, 463, 612, 671, 675, 700
LweI GCATC 3 cut(s) 208, 539, 647
MaeI CTAG 5 cut(s) 11, 201, 215, 258, 497
MaeIII GTNAC 2 cut(s) 324, 739
MalI GATC 6 cut(s) 192, 386, 432, 441, 721, 748
MboI GATC 6 cut(s) 190, 384, 430, 439, 719, 746
MboII GAAGA 5 cut(s) 146, 360, 494, 593, 690
MflI RGATCY 4 cut(s) 190, 439, 719, 746
MhlI GDGCHC 2 cut(s) 477, 496
MlsI TGGCCA 1 cut(s) 411
MluCI AATT 4 cut(s) 120, 237, 300, 682
MluNI TGGCCA 1 cut(s) 411
MlyI GAGTC 2 cut(s) 32, 158
MmeI TCCRAC 2 cut(s) 286, 375
MnlI CCTC 3 cut(s) 168, 430, 654
Mox20I TGGCCA 1 cut(s) 411
Mph1103I ATGCAT 1 cut(s) 554
MroNI GCCGGC 1 cut(s) 661
MroXI GAANNNNTTC 2 cut(s) 352, 682
MscI TGGCCA 1 cut(s) 411
MseI TTAA 2 cut(s) 404, 455
MslI CAYNNNNRTG 2 cut(s) 69, 568
Msp20I TGGCCA 1 cut(s) 411
MspI CCGG 3 cut(s) 329, 662, 687
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 2 cut(s) 608, 658
NaeI GCCGGC 1 cut(s) 663
NdeI CATATG 1 cut(s) 313
NdeII GATC 6 cut(s) 190, 384, 430, 439, 719, 746
NgoMIV GCCGGC 1 cut(s) 661
NlaIII CATG 7 cut(s) 22, 256, 325, 451, 547, 607, 625
NlaIV GGNNCC 2 cut(s) 275, 441
NmuCI GTSAC 2 cut(s) 324, 739
NsiI ATGCAT 1 cut(s) 554
NspI RCATGY 2 cut(s) 22, 325
NspV TTCGAA 1 cut(s) 587
OliI CACNNNNGTG 1 cut(s) 568
PagI TCATGA 1 cut(s) 543
PciI ACATGT 1 cut(s) 321
PdiI GCCGGC 1 cut(s) 663
PdmI GAANNNNTTC 2 cut(s) 352, 682
PfeI GAWTC 3 cut(s) 145, 509, 584
PleI GAGTC 2 cut(s) 32, 158
PpsI GAGTC 2 cut(s) 32, 158
PscI ACATGT 1 cut(s) 321
Psp124BI GAGCTC 1 cut(s) 477
PspN4I GGNNCC 2 cut(s) 275, 441
PspPI GGNCC 3 cut(s) 90, 274, 592
PsuI RGATCY 4 cut(s) 190, 439, 719, 746
RsaI GTAC 2 cut(s) 335, 576
RsaNI GTAC 2 cut(s) 334, 575
RseI CAYNNNNRTG 2 cut(s) 69, 568
SacI GAGCTC 1 cut(s) 477
SaqAI TTAA 2 cut(s) 404, 455
Sau3AI GATC 6 cut(s) 190, 384, 430, 439, 719, 746
Sau96I GGNCC 3 cut(s) 90, 274, 592
SchI GAGTC 2 cut(s) 32, 158
SduI GDGCHC 2 cut(s) 477, 496
SetI ASST 4 cut(s) 35, 477, 601, 763
SfaNI GCATC 3 cut(s) 208, 539, 647
SfuI TTCGAA 1 cut(s) 587
SinI GGWCC 3 cut(s) 90, 274, 592
SmiMI CAYNNNNRTG 2 cut(s) 69, 568
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
Sse9I AATT 4 cut(s) 120, 237, 300, 682
SsiI CCGC 1 cut(s) 338
SspI AATATT 1 cut(s) 264
SspMI CTAG 5 cut(s) 11, 201, 215, 258, 497
SstI GAGCTC 1 cut(s) 477
TaaI ACNGT 1 cut(s) 90
TaqI TCGA 2 cut(s) 587, 763
TaqII GACCGA 1 cut(s) 609
TasI AATT 4 cut(s) 120, 237, 300, 682
TatI WGTACW 1 cut(s) 574
TfiI GAWTC 3 cut(s) 145, 509, 584
Tru1I TTAA 2 cut(s) 404, 455
Tru9I TTAA 2 cut(s) 404, 455
TscAI CASTG 1 cut(s) 697
TseFI GTSAC 2 cut(s) 324, 739
Tsp45I GTSAC 2 cut(s) 324, 739
TspDTI ATGAA 4 cut(s) 242, 464, 552, 610
TspRI CASTG 1 cut(s) 697
VpaK11BI GGWCC 3 cut(s) 90, 274, 592
XapI RAATTY 2 cut(s) 237, 682
XceI RCATGY 2 cut(s) 22, 325
XcmI CCANNNNNNNNNTGG 1 cut(s) 419
XmnI GAANNNNTTC 2 cut(s) 352, 682
XspI CTAG 5 cut(s) 11, 201, 215, 258, 497
Zsp2I ATGCAT 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.