RchiOBHm_Chr5g0002911

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000112
Physical Location & Seq
Forward (+)
1703350 .. 1706688
3339 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28424

Sequence Viewer

Length: 2466 bp
ATGCCACTGTTTGCTACCTACAAAGTTGCTAATGCCCAGAAAATGTTGGTCAAAGCTCTGCTTCTCTTCCTCCTCTTTCGATTTTGCGATTCCAGGGACACCTTAACATGGAACCAAGAACAACTGAAGGATGATGGTGGAGTTTTAGTGTCCAAACAGAGCAAGTTCGAGTTGGGTTTCTTCAGCCCTGGAAATTCTAGCTACCGGTATATTGGAATTTGGTATTCTCAGACTAAAGTATCTGAGAAAACAGTGGTGTGGGTTGCAAACAGGAACAATCCCATCAATGATACCTCTGGTGTGCTCAAAATAAACAGGTATGGAGAACTGGCCCTTTATGCTTATAACATGAACAACACTCCTGTTTGGTCCACCAATGTGTCACGGCCGGTCCAAAATAATAACGCAAGCACTTTCTCTGCACAGCTTTTAGATGCAGGAAATTTAGTTGTGTTCTTGGATGATAATAATGAAATCTATGTATGGCAAAGTTTTGATTATCCTACAGATACTCTAATTCCAGGTATGAAAGTCGGGGTGAATTGGAAAACTGGGCTAGAATGGGTCTTAACATCTTGGAAGTCACAAGATGACCCTGGACTTGGGGACTATACCTTTAGGCTCTATCCAGATCAGACTGCCGCCCCCCAATATTTTATGTATAAAGGTTTGAGCAAGTATTGGCGAAGTGAGTCAGGACCAGGGCCTCCTTTTGTCACTAATCAAGAGGAAACTTATAATTTCTTCAATTACACCAATGCAATTACAAGAACGACGTTGAACGATTCTGGTAGGTTATATCGTCTTACATGGGATAATGGTGGCGTTCAATGGAAGGAAGATTTTTCTGCACCGAAGTCCCGGTGTGACTGGTATGGACAGTGTGGTGCCAACAGCAAATGTAAGCCTGACAATATTAATGAGTTTGAGTGTGAATGTTTACCAGGGTATGAGCCTAGTTCTATAAATGATTGGAATCAAAAAAATGGGTCGGGTGGATGTGTGAGTAAGCGAGTTGGTGTATCAAAGTGCGGGAGTGGAGATGGGTTTGTGAATGTGGCAAGAGTTAAATTTCCAGACACATCAATAGCATCACGGTTGAGAACAGGTATGAGTGCCAAAGAGTGTGAGCAGGACTGCTTAAGAAATTGTTCTTGCACCGCATATTTGAGCATCGAATATGAAGGGCTTGTGGATTGCTTGATATGGTATGATGACTTGATGGATATTTTAGTGTACACAGAGCGTGGACGAGAATTATACGTACGTGTAAATAAAACAGAGTTAGGAAGATCACAAGTTTTCTTGGAAAGGAGGGGTATGCTAGCTATTCCAATATTGTCTGCTCTACTGGCATTGGTCCTAATCATTATGCTTGCTTGTTGGTGGCGTAAGAAGAACAGGAACACAAAAGGCATAGACTTTGTGGAGGCAGAGGAGCTTGAGGAAACTCAGAGACACCCCGAATTGCAATTTTTCGATCTTGACACAATAATAGCAGCCACAGACCACTTCTCTCCTGTCAATGAACTTGGCCATGGTGGTTTTGGCTCTGTTTATAAGGGTCAGCTACCAAATGAACAGAAAGTTGCTGTGAAAAGATTGTCCAGAACTTCAGGACAAGGGATTGAAGAATTCAAAAATGAAGTTGCACTTATAGCAAGACTTCAACACAGGAACCTTGTGAAACTTTTAGGCTGTTGTATGAAGGGAGAAGAAAGGATGTTAGTCCTAGAATACATGCCTAACAAAAGCTTGGACTCCTTTCTTTTTGATAACACAAGACAGTCCTTCTTGGATTGGGAAAAGCGCTTTGAAATTATCAACGGGATTGCTCATGGGATTCTATATCTTCACCAAGACTCAAGATTGAGGATTATCCATAGAGATCTAAAAACTAGCAATGTTCTACTAGATGCTGAGATGAACCCAAAAATTTCGGATTTTGGCATGGCTAGAATATTCCACAGGGACCAACTGCAAGATAAGACGAACCGAATTGTCGGAACATATGGCTACATGTCACCGGAGTATGCAGTATTTGGAAGATTTTCCACAAAATCTGATGTCTTTAGTTTTGGGATCATAATGTTGGAGATTTTAAGTGGCCAGAAAAACAATGGTTTTGTTCCGGAGGATCCTTCCATGAACTTAATAAGACGTGTTTGGGAGCTCTGGGAAGAAGGCATAGCCTTGGATATTTTGGATTCGACACTGAAGTCATATCACCCTAATGAAGTCATGAGATGCATACAAGTTGCGCTCTTGTGTGTACAAGAAGATTCGAAGGACCGACCTGCCATGTCAGCTGTTGTTTTCATGTTGAGTGGTGAAGCATCTCCTCCATTGCCTAAGCAGCCGGCATTTGTTTACAGAAGAAGTTCCGGCACTGATGTTTATCCATTACTTTCAAACAGATCTTATTCTATAAACGATGTGACAGCATCTACAATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

821

Amino Acids

93.52

Weight (kDa)

6.02

Isoelectric Point (pI)

42.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 84 - 194 2.2e-35 D-mannose binding lectin
S_locus_glycop PF00954 239 - 320 4.7e-16 S-locus glycoprotein domain
PAN_2 PF08276 344 - 410 2.6e-18 PAN-like domain
Pkinase PF00069 508 - 771 5.8e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 509 - 772 4.4e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 345, 738, 1560
AasI GACNNNNNNGTC 1 cut(s) 2218
Acc36I ACCTGC 1 cut(s) 2305
AccB1I GGYRCC 1 cut(s) 887
AccIII TCCGGA 1 cut(s) 2131
AciI CCGC 3 cut(s) 642, 1032, 1161
AclWI GGATC 3 cut(s) 2090, 2132, 2145
AcoI YGGCCR 3 cut(s) 386, 1534, 2107
AcsI RAATTY 6 cut(s) 193, 216, 442, 1070, 1634, 1935
AcuI CTGAAG 4 cut(s) 146, 166, 1599, 2237
AfaI GTAC 3 cut(s) 1238, 1266, 2274
AfeI AGCGCT 1 cut(s) 1811
AfiI CCNNNNNNNGG 2 cut(s) 108, 602
AflII CTTAAG 1 cut(s) 1141
AflIII ACRYGT 3 cut(s) 1267, 2019, 2161
AgeI ACCGGT 1 cut(s) 204
AgsI TTSAA 8 cut(s) 748, 781, 830, 1631, 1639, 1670, 1817, 2412
AjiI CACGTC 1 cut(s) 2162
AjnI CCWGG 6 cut(s) 92, 187, 520, 595, 700, 943
AleI CACNNNNGTG 1 cut(s) 377
Alw21I GWGCWC 2 cut(s) 306, 2175
Alw26I GTCTC 1 cut(s) 1450
AlwI GGATC 3 cut(s) 2090, 2132, 2145
Aor13HI TCCGGA 1 cut(s) 2131
Aor51HI AGCGCT 1 cut(s) 1811
AoxI GGCC 5 cut(s) 330, 386, 704, 1534, 2107
ApeKI GCWGC 2 cut(s) 1499, 2356
ApoI RAATTY 6 cut(s) 193, 216, 442, 1070, 1634, 1935
AseI ATTAAT 1 cut(s) 918
AsiGI ACCGGT 1 cut(s) 204
Asp700I GAANNNNTTC 3 cut(s) 1150, 2050, 2380
AspLEI GCGC 2 cut(s) 1812, 2263
AspS9I GGNCC 8 cut(s) 331, 369, 391, 698, 704, 1360, 1972, 2290
AsuC2I CCSGG 1 cut(s) 862
AsuHPI GGTGA 5 cut(s) 550, 1847, 2016, 2219, 2342
AsuII TTCGAA 1 cut(s) 2285
AsuNHI GCTAGC 1 cut(s) 1324
AvaII GGWCC 6 cut(s) 369, 391, 698, 1360, 1972, 2290
BaeI ACNNNNGTAYC 2 cut(s) 222, 255
BalI TGGCCA 2 cut(s) 1536, 2109
BamHI GGATCC 1 cut(s) 2137
BanI GGYRCC 1 cut(s) 887
BanII GRGCYC 1 cut(s) 2175
Bbv12I GWGCWC 2 cut(s) 306, 2175
BbvI GCAGC 2 cut(s) 1511, 2368
BccI CCATC 4 cut(s) 128, 290, 1037, 1216
BceAI ACGGC 1 cut(s) 401
BciT130I CCWGG 6 cut(s) 94, 189, 522, 597, 702, 945
BcnI CCSGG 1 cut(s) 862
BcoDI GTCTC 1 cut(s) 1450
BfaI CTAG 8 cut(s) 198, 557, 957, 1325, 1733, 1899, 1913, 1956
BfmI CTRYAG 1 cut(s) 504
BfoI RGCGCY 1 cut(s) 1813
BfrI CTTAAG 1 cut(s) 1141
BfuAI ACCTGC 1 cut(s) 2305
BglII AGATCT 2 cut(s) 1888, 2417
BisI GCNGC 3 cut(s) 642, 1500, 2357
BlsI GCNGC 3 cut(s) 643, 1501, 2358
Bme1390I CCNGG 7 cut(s) 94, 189, 522, 597, 702, 862, 945
Bme18I GGWCC 6 cut(s) 369, 391, 698, 1360, 1972, 2290
BmgBI CACGTC 1 cut(s) 2162
BmgT120I GGNCC 8 cut(s) 331, 369, 391, 698, 704, 1360, 1972, 2290
BmiI GGNNCC 5 cut(s) 113, 889, 1679, 1973, 2139
BmrFI CCNGG 7 cut(s) 94, 189, 522, 597, 702, 862, 945
BmrI ACTGGG 1 cut(s) 561
BmsI GCATC 7 cut(s) 424, 1100, 1182, 1906, 2237, 2345, 2453
BmtI GCTAGC 1 cut(s) 1328
BmuI ACTGGG 1 cut(s) 561
Bpu10I CCTNAGC 1 cut(s) 2352
Bpu14I TTCGAA 1 cut(s) 2285
BpuEI CTTGAG 2 cut(s) 1463, 1849
BpuMI CCSGG 1 cut(s) 862
BsaAI YACGTR 2 cut(s) 1264, 1268
BsaBI GATNNNNATC 2 cut(s) 975, 2397
BsaJI CCNNGG 7 cut(s) 93, 187, 595, 701, 944, 1537, 2193
BsaWI WCCGGW 3 cut(s) 204, 2026, 2131
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 602
Bse118I RCCGGY 3 cut(s) 204, 388, 2359
Bse1I ACTGG 4 cut(s) 333, 556, 875, 1356
Bse3DI GCAATG 2 cut(s) 1909, 2345
Bse8I GATNNNNATC 2 cut(s) 975, 2397
BseAI TCCGGA 1 cut(s) 2131
BseBI CCWGG 6 cut(s) 94, 189, 522, 597, 702, 945
BseDI CCNNGG 7 cut(s) 93, 187, 595, 701, 944, 1537, 2193
BseGI GGATG 4 cut(s) 136, 466, 1004, 1728
BseJI GATNNNNATC 2 cut(s) 975, 2397
BseLI CCNNNNNNNGG 2 cut(s) 108, 602
BseMI GCAATG 2 cut(s) 1909, 2345
BseMII CTCAG 4 cut(s) 234, 242, 1466, 1911
BseNI ACTGG 4 cut(s) 333, 556, 875, 1356
BseRI GAGGAG 3 cut(s) 62, 1451, 2331
BseX3I CGGCCG 1 cut(s) 386
BseXI GCAGC 2 cut(s) 1511, 2368
BsgI GTGCAG 2 cut(s) 405, 834
Bsh1285I CGRYCG 1 cut(s) 389
BshFI GGCC 5 cut(s) 332, 388, 706, 1536, 2109
BshNI GGYRCC 1 cut(s) 887
BshTI ACCGGT 1 cut(s) 204
BsiEI CGRYCG 1 cut(s) 389
BsiHKAI GWGCWC 2 cut(s) 306, 2175
BsiSI CCGG 7 cut(s) 205, 389, 862, 2027, 2132, 2360, 2385
BsiWI CGTACG 1 cut(s) 1264
BslFI GGGAC 4 cut(s) 110, 620, 844, 1985
BslI CCNNNNNNNGG 2 cut(s) 108, 602
BsmAI GTCTC 1 cut(s) 1450
BsmFI GGGAC 4 cut(s) 110, 620, 844, 1985
BsnI GGCC 5 cut(s) 332, 388, 706, 1536, 2109
Bsp119I TTCGAA 1 cut(s) 2285
Bsp1286I GDGCHC 2 cut(s) 306, 2175
Bsp13I TCCGGA 1 cut(s) 2131
Bsp1407I TGTACA 2 cut(s) 1236, 2272
Bsp143I GATC 7 cut(s) 631, 1292, 1480, 1888, 2082, 2137, 2417
Bsp19I CCATGG 1 cut(s) 1537
BspACI CCGC 3 cut(s) 642, 1032, 1161
BspANI GGCC 5 cut(s) 332, 388, 706, 1536, 2109
BspCNI CTCAG 4 cut(s) 235, 241, 1465, 1912
BspEI TCCGGA 1 cut(s) 2131
BspHI TCATGA 1 cut(s) 2241
BspLI GGNNCC 5 cut(s) 113, 889, 1679, 1973, 2139
BspMI ACCTGC 1 cut(s) 2305
BspOI GCTAGC 1 cut(s) 1328
BspPI GGATC 3 cut(s) 2090, 2132, 2145
BspT104I TTCGAA 1 cut(s) 2285
BspT107I GGYRCC 1 cut(s) 887
BspTI CTTAAG 1 cut(s) 1141
BsrDI GCAATG 2 cut(s) 1909, 2345
BsrFI RCCGGY 3 cut(s) 204, 388, 2359
BsrGI TGTACA 2 cut(s) 1236, 2272
BsrI ACTGG 4 cut(s) 333, 556, 875, 1356
BssAI RCCGGY 3 cut(s) 204, 388, 2359
BssECI CCNNGG 7 cut(s) 93, 187, 595, 701, 944, 1537, 2193
BssMI GATC 7 cut(s) 631, 1292, 1480, 1888, 2082, 2137, 2417
BssT1I CCWWGG 2 cut(s) 1537, 2193
Bst2UI CCWGG 6 cut(s) 94, 189, 522, 597, 702, 945
Bst4CI ACNGT 5 cut(s) 9, 253, 882, 1098, 1788
Bst6I CTCTTC 1 cut(s) 71
BstAFI CTTAAG 1 cut(s) 1141
BstAUI TGTACA 2 cut(s) 1236, 2272
BstBAI YACGTR 2 cut(s) 1264, 1268
BstBI TTCGAA 1 cut(s) 2285
BstC8I GCNNGC 4 cut(s) 409, 1326, 1377, 2361
BstDEI CTNAG 5 cut(s) 228, 243, 1452, 1920, 2352
BstDSI CCRYGG 1 cut(s) 1537
BstF5I GGATG 4 cut(s) 136, 466, 1004, 1728
BstH2I RGCGCY 1 cut(s) 1813
BstHHI GCGC 2 cut(s) 1812, 2263
BstKTI GATC 7 cut(s) 634, 1295, 1483, 1891, 2085, 2140, 2420
BstMAI GTCTC 1 cut(s) 1450
BstMBI GATC 7 cut(s) 631, 1292, 1480, 1888, 2082, 2137, 2417
BstMCI CGRYCG 1 cut(s) 389
BstMWI GCNNNNNNNGC 5 cut(s) 338, 1352, 1658, 2306, 2356
BstNI CCWGG 6 cut(s) 94, 189, 522, 597, 702, 945
BstNSI RCATGY 2 cut(s) 1744, 2023
BstSCI CCNGG 7 cut(s) 92, 187, 520, 595, 700, 860, 943
BstSFI CTRYAG 1 cut(s) 504
BstSNI TACGTA 1 cut(s) 1264
BstV1I GCAGC 2 cut(s) 1511, 2368
BstX2I RGATCY 3 cut(s) 1888, 2137, 2417
BstYI RGATCY 3 cut(s) 1888, 2137, 2417
BstZI CGGCCG 1 cut(s) 386
BsuRI GGCC 5 cut(s) 332, 388, 706, 1536, 2109
BtgI CCRYGG 1 cut(s) 1537
BtrI CACGTC 1 cut(s) 2162
BtsCI GGATG 4 cut(s) 136, 466, 1004, 1728
BtsIMutI CAGTG 5 cut(s) 5, 258, 887, 2213, 2388
BveI ACCTGC 1 cut(s) 2305
Cac8I GCNNGC 4 cut(s) 409, 1326, 1377, 2361
CciI TCATGA 1 cut(s) 2241
CfoI GCGC 2 cut(s) 1812, 2263
Cfr10I RCCGGY 3 cut(s) 204, 388, 2359
Cfr13I GGNCC 8 cut(s) 331, 369, 391, 698, 704, 1360, 1972, 2290
Csp6I GTAC 3 cut(s) 1237, 1265, 2273
CspAI ACCGGT 1 cut(s) 204
CviQI GTAC 3 cut(s) 1237, 1265, 2273
DdeI CTNAG 5 cut(s) 228, 243, 1452, 1920, 2352
DpnI GATC 7 cut(s) 633, 1294, 1482, 1890, 2084, 2139, 2419
DpnII GATC 7 cut(s) 631, 1292, 1480, 1888, 2082, 2137, 2417
DrdI GACNNNNNNGTC 1 cut(s) 2218
DseDI GACNNNNNNGTC 1 cut(s) 2218
EaeI YGGCCR 3 cut(s) 386, 1534, 2107
EagI CGGCCG 1 cut(s) 386
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Ecl136II GAGCTC 1 cut(s) 2173
EclXI CGGCCG 1 cut(s) 386
Eco105I TACGTA 1 cut(s) 1264
Eco130I CCWWGG 2 cut(s) 1537, 2193
Eco24I GRGCYC 1 cut(s) 2175
Eco47I GGWCC 6 cut(s) 369, 391, 698, 1360, 1972, 2290
Eco47III AGCGCT 1 cut(s) 1811
Eco52I CGGCCG 1 cut(s) 386
Eco53kI GAGCTC 1 cut(s) 2173
Eco57I CTGAAG 4 cut(s) 146, 166, 1599, 2237
EcoICRI GAGCTC 1 cut(s) 2173
EcoO109I RGGNCCY 1 cut(s) 704
EcoRI GAATTC 1 cut(s) 1634
EcoRII CCWGG 6 cut(s) 92, 187, 520, 595, 700, 943
EcoT14I CCWWGG 2 cut(s) 1537, 2193
EcoT22I ATGCAT 1 cut(s) 2252
EcoT38I GRGCYC 1 cut(s) 2175
ErhI CCWWGG 2 cut(s) 1537, 2193
FalI AAGNNNNNCTT 4 cut(s) 45, 77, 1638, 1670
FaqI GGGAC 4 cut(s) 110, 620, 844, 1985
FauI CCCGC 1 cut(s) 1025
FauNDI CATATG 1 cut(s) 2011
Fnu4HI GCNGC 3 cut(s) 642, 1500, 2357
FokI GGATG 4 cut(s) 143, 473, 1011, 1735
FriOI GRGCYC 1 cut(s) 2175
Fsp4HI GCNGC 3 cut(s) 642, 1500, 2357
FspBI CTAG 8 cut(s) 198, 557, 957, 1325, 1733, 1899, 1913, 1956
GlaI GCGC 2 cut(s) 1811, 2262
GluI GCNGC 3 cut(s) 642, 1500, 2357
HaeII RGCGCY 1 cut(s) 1813
HaeIII GGCC 5 cut(s) 332, 388, 706, 1536, 2109
HapII CCGG 7 cut(s) 205, 389, 862, 2027, 2132, 2360, 2385
HhaI GCGC 2 cut(s) 1812, 2263
Hin6I GCGC 2 cut(s) 1810, 2261
HinP1I GCGC 2 cut(s) 1810, 2261
HindIII AAGCTT 1 cut(s) 1753
HinfI GANTC 9 cut(s) 89, 692, 785, 976, 1760, 1843, 1862, 2207, 2282
HpaII CCGG 7 cut(s) 205, 389, 862, 2027, 2132, 2360, 2385
HphI GGTGA 5 cut(s) 550, 1847, 2016, 2219, 2342
Hpy166II GTNNAC 7 cut(s) 372, 941, 1237, 1239, 1250, 2273, 2371
Hpy188I TCNGA 7 cut(s) 231, 244, 636, 1455, 1942, 2006, 2065
Hpy8I GTNNAC 7 cut(s) 372, 941, 1237, 1239, 1250, 2273, 2371
Hpy99I CGWCG 1 cut(s) 778
HpyAV CCTTC 8 cut(s) 121, 829, 1178, 1702, 1801, 2151, 2177, 2281
HpyCH4III ACNGT 5 cut(s) 9, 253, 882, 1098, 1788
HpyCH4IV ACGT 4 cut(s) 776, 1263, 1267, 2161
HpyF10VI GCNNNNNNNGC 5 cut(s) 338, 1352, 1658, 2306, 2356
HpyF3I CTNAG 5 cut(s) 228, 243, 1452, 1920, 2352
HpySE526I ACGT 4 cut(s) 776, 1263, 1267, 2161
HspAI GCGC 2 cut(s) 1810, 2261
Kpn2I TCCGGA 1 cut(s) 2131
KroI GCCGGC 1 cut(s) 2359
KroNI GCCGGC 1 cut(s) 2361
Kzo9I GATC 7 cut(s) 631, 1292, 1480, 1888, 2082, 2137, 2417
LmnI GCTCC 2 cut(s) 1438, 2170
Lsp1109I GCAGC 2 cut(s) 1511, 2368
LweI GCATC 7 cut(s) 424, 1100, 1182, 1906, 2237, 2345, 2453
MaeI CTAG 8 cut(s) 198, 557, 957, 1325, 1733, 1899, 1913, 1956
MaeII ACGT 4 cut(s) 776, 1263, 1267, 2161
MaeIII GTNAC 6 cut(s) 381, 582, 715, 866, 2022, 2437
MalI GATC 7 cut(s) 633, 1294, 1482, 1890, 2084, 2139, 2419
MboI GATC 7 cut(s) 631, 1292, 1480, 1888, 2082, 2137, 2417
MflI RGATCY 3 cut(s) 1888, 2137, 2417
MhlI GDGCHC 2 cut(s) 306, 2175
MlsI TGGCCA 2 cut(s) 1536, 2109
MluNI TGGCCA 2 cut(s) 1536, 2109
MlyI GAGTC 3 cut(s) 701, 1754, 1856
MmeI TCCRAC 2 cut(s) 1984, 2073
Mox20I TGGCCA 2 cut(s) 1536, 2109
Mph1103I ATGCAT 1 cut(s) 2252
MroI TCCGGA 1 cut(s) 2131
MroNI GCCGGC 1 cut(s) 2359
MroXI GAANNNNTTC 3 cut(s) 1150, 2050, 2380
MscI TGGCCA 2 cut(s) 1536, 2109
MseI TTAA 7 cut(s) 104, 569, 918, 1068, 1142, 2102, 2153
MslI CAYNNNNRTG 2 cut(s) 377, 2232
Msp20I TGGCCA 2 cut(s) 1536, 2109
MspA1I CMGCKG 1 cut(s) 2309
MspCI CTTAAG 1 cut(s) 1141
MspI CCGG 7 cut(s) 205, 389, 862, 2027, 2132, 2360, 2385
MspR9I CCNGG 7 cut(s) 94, 189, 522, 597, 702, 862, 945
MvaI CCWGG 6 cut(s) 94, 189, 522, 597, 702, 945
MwoI GCNNNNNNNGC 5 cut(s) 338, 1352, 1658, 2306, 2356
NaeI GCCGGC 1 cut(s) 2361
NciI CCSGG 1 cut(s) 862
NcoI CCATGG 1 cut(s) 1537
NdeI CATATG 1 cut(s) 2011
NdeII GATC 7 cut(s) 631, 1292, 1480, 1888, 2082, 2137, 2417
NgoMIV GCCGGC 1 cut(s) 2359
NheI GCTAGC 1 cut(s) 1324
NlaIV GGNNCC 5 cut(s) 113, 889, 1679, 1973, 2139
NmuCI GTSAC 6 cut(s) 381, 582, 715, 866, 2022, 2437
NsiI ATGCAT 1 cut(s) 2252
NspI RCATGY 2 cut(s) 1744, 2023
NspV TTCGAA 1 cut(s) 2285
OliI CACNNNNGTG 1 cut(s) 377
PagI TCATGA 1 cut(s) 2241
PciI ACATGT 1 cut(s) 2019
PdiI GCCGGC 1 cut(s) 2361
PdmI GAANNNNTTC 3 cut(s) 1150, 2050, 2380
PfeI GAWTC 6 cut(s) 89, 785, 976, 1843, 2207, 2282
Pfl23II CGTACG 1 cut(s) 1264
PinAI ACCGGT 1 cut(s) 204
PkrI GCNGC 3 cut(s) 643, 1501, 2358
PleI GAGTC 3 cut(s) 700, 1754, 1856
PpsI GAGTC 3 cut(s) 700, 1754, 1856
Ppu21I YACGTR 2 cut(s) 1264, 1268
PscI ACATGT 1 cut(s) 2019
PshBI ATTAAT 1 cut(s) 918
PsiI TTATAA 3 cut(s) 345, 738, 1560
Psp124BI GAGCTC 1 cut(s) 2175
Psp6I CCWGG 6 cut(s) 92, 187, 520, 595, 700, 943
PspGI CCWGG 6 cut(s) 92, 187, 520, 595, 700, 943
PspLI CGTACG 1 cut(s) 1264
PspN4I GGNNCC 5 cut(s) 113, 889, 1679, 1973, 2139
PspPI GGNCC 8 cut(s) 331, 369, 391, 698, 704, 1360, 1972, 2290
PsuI RGATCY 3 cut(s) 1888, 2137, 2417
PvuII CAGCTG 1 cut(s) 2309
RsaI GTAC 3 cut(s) 1238, 1266, 2274
RsaNI GTAC 3 cut(s) 1237, 1265, 2273
RseI CAYNNNNRTG 2 cut(s) 377, 2232
SacI GAGCTC 1 cut(s) 2175
SaqAI TTAA 7 cut(s) 104, 569, 918, 1068, 1142, 2102, 2153
SatI GCNGC 3 cut(s) 642, 1500, 2357
Sau3AI GATC 7 cut(s) 631, 1292, 1480, 1888, 2082, 2137, 2417
Sau96I GGNCC 8 cut(s) 331, 369, 391, 698, 704, 1360, 1972, 2290
SchI GAGTC 3 cut(s) 701, 1754, 1856
ScrFI CCNGG 7 cut(s) 94, 189, 522, 597, 702, 862, 945
SduI GDGCHC 2 cut(s) 306, 2175
SfaNI GCATC 7 cut(s) 424, 1100, 1182, 1906, 2237, 2345, 2453
SfcI CTRYAG 1 cut(s) 504
SfuI TTCGAA 1 cut(s) 2285
SinI GGWCC 6 cut(s) 369, 391, 698, 1360, 1972, 2290
SmiMI CAYNNNNRTG 2 cut(s) 377, 2232
SmlI CTYRAG 3 cut(s) 1141, 1442, 1864
SmoI CTYRAG 3 cut(s) 1141, 1442, 1864
SnaBI TACGTA 1 cut(s) 1264
SsiI CCGC 3 cut(s) 642, 1032, 1161
SspI AATATT 4 cut(s) 653, 916, 1338, 1962
SspMI CTAG 8 cut(s) 198, 557, 957, 1325, 1733, 1899, 1913, 1956
SstI GAGCTC 1 cut(s) 2175
StyD4I CCNGG 7 cut(s) 92, 187, 520, 595, 700, 860, 943
StyI CCWWGG 2 cut(s) 1537, 2193
TaaI ACNGT 5 cut(s) 9, 253, 882, 1098, 1788
TaiI ACGT 4 cut(s) 779, 1266, 1270, 2164
TaqI TCGA 7 cut(s) 79, 168, 1176, 1479, 2210, 2285, 2461
TaqII GACCGA 1 cut(s) 2307
TatI WGTACW 2 cut(s) 1236, 2272
TauI GCSGC 1 cut(s) 644
TfiI GAWTC 6 cut(s) 89, 785, 976, 1843, 2207, 2282
Tru1I TTAA 7 cut(s) 104, 569, 918, 1068, 1142, 2102, 2153
Tru9I TTAA 7 cut(s) 104, 569, 918, 1068, 1142, 2102, 2153
TscAI CASTG 5 cut(s) 12, 258, 887, 2220, 2395
TseFI GTSAC 6 cut(s) 381, 582, 715, 866, 2022, 2437
TseI GCWGC 2 cut(s) 1499, 2356
Tsp45I GTSAC 6 cut(s) 381, 582, 715, 866, 2022, 2437
TspRI CASTG 5 cut(s) 12, 258, 887, 2220, 2395
Vha464I CTTAAG 1 cut(s) 1141
VpaK11BI GGWCC 6 cut(s) 369, 391, 698, 1360, 1972, 2290
VspI ATTAAT 1 cut(s) 918
XapI RAATTY 6 cut(s) 193, 216, 442, 1070, 1634, 1935
XceI RCATGY 2 cut(s) 1744, 2023
XcmI CCANNNNNNNNNTGG 2 cut(s) 1544, 2117
XmnI GAANNNNTTC 3 cut(s) 1150, 2050, 2380
XspI CTAG 8 cut(s) 198, 557, 957, 1325, 1733, 1899, 1913, 1956
Zsp2I ATGCAT 1 cut(s) 2252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.