Rroxscaffold_2G00111810

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000112
Physical Location & Seq
Reverse (-)
36604711 .. 36608065
3355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00111810.1

Sequence Viewer

Length: 2601 bp
ATGCCACTCATCTCATTTATCACCAGCTCAAGTATGAAAATGAGAAAAAAGATGCCATTGGTTCATTCCTACAGAATTGAAAATGCCGAGAAAATGGTTTTGAAAGCTCTGCTTCTCTTCCTCCTCTTTCAATTATGCACTTCCAGGGACACCATGAGAGGGAACGAAGAACAATTGAAGGATGTTGATGGTGACTATTTAGTGTCCAAAGAAAGCAAGTTTGAATTGGGTTTCTTCAGCCCCGGAAATTCTAGCAACCGGTATGTTGGAGTCTGGTATTCTCAGACTAGAGTATCTAATAAAACAGTGGTGTGGGTTGCAAACAGGAACAATCCTATCAATGATACCTCTGGTGTGCTCACAATAAACAGGTATGGAGAACTAGTCCTATATGCTCATAACATGGAAAGCACTCCTATTTGGTCTACTAACGTGTCGCGGTCGGTTCAAAATGTTAACACAAGCACTTCATCTACACAACTTTTAGATACAGGAAATTTAGTTGTGTTCCAGGATGATAAAAATGAAATCTTTATATGGCAAAGTTTTGATCATCCTATAGATACTTTAATTCCAGGTATGAAAGTTGGGGTGAATTGGAAAACTGGGCAAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACCTATAGGCTAAGTTCAGATCATAATGCATCTCCCCAATTTTTTTTGTATAAAGGTTTGAGTAAGTATTGGCGAAGTGATCCAGGGCCAGCGCCTACTTTGGTCTCTAATCAAGATGAAACTTATTATTTCATGAATGAAACCAATGCAATTACAAGAATAATAGTGACTGATTCTACCTTAGAGCACCTTATATGGGATGGTGGTGGCCTTCAATGGAAGGAAGACTTCTCTGCACCGAAGTCCCGGTGTGACAGGTACGGACAGTGTGGTGCCAACAGCAGATGTAGCCCTGACAATGTTAATCTGTTTGAGTGTGACTGTTTGCCAGGGTATGTGCCTAATTCTGTAAGTGATTGGAATCAGAAAAATGGTTCAGGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAAGTGTGGAGATGGAGACGGGTTTATAAAGGTGGCAAGAGTTAAATATCCAGACACATCGATAGCAGCATCGTTAAAATCAGGTATGAGTGACAAAGAGTGTGCGCAGGAGTGCCTAAGAAATTGTTCTTGCACTGCATATTTGAGCACTGAAAATGAAGGGATTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTTTAGTGTACACAGAGCTTGGACGAGACCTCTATGTTCGTGTGAATGCAACTGTGTTAGCGGCAAATGCTGGAAGATCACATGGTTTCTTGGAAAGGAGGGGTATGCTGGCTATTCCAATACTGTCTACTGCGCTAGCATTGGTACTAATCATTATGCTTGCATGGAAGTGGCGTAAGAAGAACAGGAACACAAGAGGCATAGACTTTGTGGAGGCAGAGGAGCTTGAGGAAACTCAGAGACACCCCGAATTGCAATTTTTTGATCTTGACACAATAATAGCAGCCACAGACCACTTCTCTCGTGTCAATGAACTTGGCCGCGGTGGCTTTGGCTCTGTTTATAAGGGTCAGCTATCAAATGAACAGAAAGTTGCTGTGAAAAGATTGTCCAAAACTTCAGGACAAGGGATTGGAGAATTTAAGAATGAAGTTGCACTTATAGCAAGACTTCAACACAGGAACCTTGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATGTTAGTCCTAGAATACATGCCTAACAAAGGCTTGGACTCCTTTCTTTTTGATAAGACAAGACGGTCCTTCTTGAATTGGGAAAAGCGTTTTGAAATTATCAATGGGATTGCTCGTGGGATTCTGTATCTTCACCAAGACTCAAGATTGAGGATTATTCATAGAGATCTAAAAACTAGTAATGTTCTACTAGATGATGAGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATATTCCATGGAGACCAACTGCAAGATAAGACGAGCCGAATTGTTGGAACATATGGCTACATGTCACCAGAGTACGCTGTATTTGGGAGATTTTCCACAAAGTCCGATGTCTTTAGTTTTGGGATCATAATGTTGGAGATTGTAAGTGGCCAGAAAAACAATGGTTCTGATCCGGAGGATCCTTCCATGAACTTAATAGGACGTGTAAGCAACACATATCTTTCTAACATTTTATCTATGAGTATGTATTTGTTTTTTAAACTAAATTTCAACTGCAGGGTATGGGAGCTCGGAGAGAAGGCGAGCCTTGGATATTGTGGATTCAACACGAAGTCATATCATCCAAATGAAGCCATGAGGTGCATACAAGTTGCGCTCTTGTGTGTACAAGAAGATCCGAATGACCGACCTGCCATGTCAGCCATTGTTTTCATGTTGAGTGATGAAGCATCTCCTCCATTGCCAAAGGAGTCGGCATTTGTTTACATAAGAAATTTCGGCACTCATGTTGATCCATTACTTTCAAACAGATCTTCTTCTATAAATGACTTGACAGTAACTACAATGAAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

866

Amino Acids

97.8

Weight (kDa)

6.57

Isoelectric Point (pI)

35.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 102 - 212 4e-35 D-mannose binding lectin
S_locus_glycop PF00954 261 - 337 1.7e-12 S-locus glycoprotein domain
PAN_2 PF08276 361 - 427 4.2e-17 PAN-like domain
Pkinase PF00069 527 - 816 7.9e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 529 - 817 5.6e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1103, 1623
AasI GACNNNNNNGTC 1 cut(s) 1849
Acc16I TGCGCA 1 cut(s) 1182
Acc36I ACCTGC 1 cut(s) 2440
AccB1I GGYRCC 1 cut(s) 938
AccI GTMKAC 2 cut(s) 425, 1406
AccII CGCG 2 cut(s) 439, 1602
AccIII TCCGGA 1 cut(s) 2194
AciI CCGC 4 cut(s) 439, 1340, 1600, 1602
AclWI GGATC 7 cut(s) 740, 2153, 2186, 2195, 2208, 2411, 2528
AcoI YGGCCR 2 cut(s) 1597, 2170
AcsI RAATTY 6 cut(s) 247, 496, 1697, 1998, 2287, 2515
AcuI CTGAAG 2 cut(s) 220, 1662
AfaI GTAC 5 cut(s) 926, 1289, 1425, 2096, 2409
AfiI CCNNNNNNNGG 4 cut(s) 159, 656, 862, 1814
AflIII ACRYGT 3 cut(s) 432, 2082, 2224
AgeI ACCGGT 1 cut(s) 258
AhlI ACTAGT 2 cut(s) 382, 1961
AjiI CACGTC 1 cut(s) 2225
AjnI CCWGG 6 cut(s) 143, 510, 574, 649, 748, 994
AluBI AGCT 7 cut(s) 27, 107, 1297, 1504, 1633, 2311, 2594
AluI AGCT 7 cut(s) 27, 107, 1297, 1504, 1633, 2311, 2594
Alw21I GWGCWC 4 cut(s) 360, 855, 1226, 2313
Alw26I GTCTC 5 cut(s) 775, 1086, 1299, 1513, 2028
AlwI GGATC 7 cut(s) 740, 2153, 2186, 2195, 2208, 2411, 2528
Aor13HI TCCGGA 1 cut(s) 2194
AoxI GGCC 4 cut(s) 752, 874, 1597, 2170
ApeKI GCWGC 2 cut(s) 1142, 1562
ApoI RAATTY 6 cut(s) 247, 496, 1697, 1998, 2287, 2515
AsiGI ACCGGT 1 cut(s) 258
Asp700I GAANNNNTTC 1 cut(s) 1201
AspLEI GCGC 4 cut(s) 760, 1183, 1414, 2398
AspS9I GGNCC 2 cut(s) 752, 1851
AsuC2I CCSGG 2 cut(s) 243, 913
AsuHPI GGTGA 5 cut(s) 13, 203, 604, 1910, 2079
AsuNHI GCTAGC 1 cut(s) 1414
AvaII GGWCC 1 cut(s) 1851
BaeI ACNNNNGTAYC 2 cut(s) 276, 309
BalI TGGCCA 1 cut(s) 2172
BamHI GGATCC 1 cut(s) 2200
BanI GGYRCC 1 cut(s) 938
BanII GRGCYC 1 cut(s) 2313
BauI CACGAG 2 cut(s) 1581, 1899
BbsI GAAGAC 1 cut(s) 897
Bbv12I GWGCWC 4 cut(s) 360, 855, 1226, 2313
BbvI GCAGC 2 cut(s) 1154, 1574
BccI CCATC 4 cut(s) 182, 860, 1082, 1267
BciT130I CCWGG 6 cut(s) 145, 512, 576, 651, 750, 996
BclI TGATCA 1 cut(s) 550
BcnI CCSGG 2 cut(s) 243, 913
BcoDI GTCTC 5 cut(s) 775, 1086, 1299, 1513, 2028
BcuI ACTAGT 2 cut(s) 382, 1961
BfaI CTAG 8 cut(s) 252, 288, 383, 1415, 1796, 1962, 1976, 2019
BfmI CTRYAG 4 cut(s) 70, 558, 670, 2296
BfoI RGCGCY 1 cut(s) 761
BfuAI ACCTGC 1 cut(s) 2440
BglI GCCNNNNNGGC 1 cut(s) 1605
BglII AGATCT 2 cut(s) 1951, 2552
BisI GCNGC 4 cut(s) 1143, 1341, 1563, 1600
BlsI GCNGC 4 cut(s) 1144, 1342, 1564, 1601
Bme1390I CCNGG 8 cut(s) 145, 243, 512, 576, 651, 750, 913, 996
Bme18I GGWCC 1 cut(s) 1851
BmgBI CACGTC 1 cut(s) 2225
BmgT120I GGNCC 2 cut(s) 752, 1851
BmiI GGNNCC 3 cut(s) 940, 1742, 2202
BmrFI CCNGG 8 cut(s) 145, 243, 512, 576, 651, 750, 913, 996
BmrI ACTGGG 2 cut(s) 615, 666
BmsI GCATC 4 cut(s) 42, 704, 1154, 2480
BmtI GCTAGC 1 cut(s) 1418
BmuI ACTGGG 2 cut(s) 615, 666
BpiI GAAGAC 1 cut(s) 897
BpuEI CTTGAG 3 cut(s) 13, 1526, 1912
BpuMI CCSGG 2 cut(s) 243, 913
Bsa29I ATCGAT 1 cut(s) 1136
BsaBI GATNNNNATC 1 cut(s) 1026
BsaI GGTCTC 3 cut(s) 775, 1299, 2028
BsaJI CCNNGG 8 cut(s) 144, 241, 649, 749, 995, 1600, 2029, 2329
BsaWI WCCGGW 2 cut(s) 258, 2194
BsaXI ACNNNNNCTCC 2 cut(s) 369, 399
Bsc4I CCNNNNNNNGG 4 cut(s) 159, 656, 862, 1814
Bse118I RCCGGY 1 cut(s) 258
Bse1I ACTGG 2 cut(s) 610, 661
Bse3DI GCAATG 1 cut(s) 2480
Bse8I GATNNNNATC 1 cut(s) 1026
BseAI TCCGGA 1 cut(s) 2194
BseBI CCWGG 6 cut(s) 145, 512, 576, 651, 750, 996
BseCI ATCGAT 1 cut(s) 1136
BseDI CCNNGG 8 cut(s) 144, 241, 649, 749, 995, 1600, 2029, 2329
BseGI GGATG 7 cut(s) 187, 520, 553, 871, 1055, 1791, 2362
BseJI GATNNNNATC 1 cut(s) 1026
BseLI CCNNNNNNNGG 4 cut(s) 159, 656, 862, 1814
BseMI GCAATG 1 cut(s) 2480
BseMII CTCAG 2 cut(s) 296, 1529
BseNI ACTGG 2 cut(s) 610, 661
BseRI GAGGAG 3 cut(s) 113, 1514, 2466
BseXI GCAGC 2 cut(s) 1154, 1574
BsgI GTGCAG 1 cut(s) 885
Bsh1236I CGCG 2 cut(s) 439, 1602
Bsh1285I CGRYCG 1 cut(s) 443
BshFI GGCC 4 cut(s) 754, 876, 1599, 2172
BshNI GGYRCC 1 cut(s) 938
BshTI ACCGGT 1 cut(s) 258
BshVI ATCGAT 1 cut(s) 1136
BsiEI CGRYCG 1 cut(s) 443
BsiHKAI GWGCWC 4 cut(s) 360, 855, 1226, 2313
BsiSI CCGG 4 cut(s) 243, 259, 913, 2195
BslFI GGGAC 3 cut(s) 161, 674, 895
BslI CCNNNNNNNGG 4 cut(s) 159, 656, 862, 1814
BsmAI GTCTC 5 cut(s) 775, 1086, 1299, 1513, 2028
BsmBI CGTCTC 1 cut(s) 1086
BsmFI GGGAC 3 cut(s) 161, 674, 895
BsmI GAATGC 1 cut(s) 1330
BsnI GGCC 4 cut(s) 754, 876, 1599, 2172
Bso31I GGTCTC 3 cut(s) 775, 1299, 2028
Bsp1286I GDGCHC 4 cut(s) 360, 855, 1226, 2313
Bsp13I TCCGGA 1 cut(s) 2194
Bsp1407I TGTACA 2 cut(s) 1287, 2407
Bsp19I CCATGG 1 cut(s) 2029
BspACI CCGC 4 cut(s) 439, 1340, 1600, 1602
BspANI GGCC 4 cut(s) 754, 876, 1599, 2172
BspCNI CTCAG 2 cut(s) 295, 1528
BspDI ATCGAT 1 cut(s) 1136
BspEI TCCGGA 1 cut(s) 2194
BspFNI CGCG 2 cut(s) 439, 1602
BspHI TCATGA 1 cut(s) 798
BspLI GGNNCC 3 cut(s) 940, 1742, 2202
BspMAI CTGCAG 1 cut(s) 2300
BspMI ACCTGC 1 cut(s) 2440
BspOI GCTAGC 1 cut(s) 1418
BspPI GGATC 7 cut(s) 740, 2153, 2186, 2195, 2208, 2411, 2528
BspT107I GGYRCC 1 cut(s) 938
BspTNI GGTCTC 3 cut(s) 775, 1299, 2028
BsrDI GCAATG 1 cut(s) 2480
BsrFI RCCGGY 1 cut(s) 258
BsrGI TGTACA 2 cut(s) 1287, 2407
BsrI ACTGG 2 cut(s) 610, 661
BssAI RCCGGY 1 cut(s) 258
BssECI CCNNGG 8 cut(s) 144, 241, 649, 749, 995, 1600, 2029, 2329
BssSI CACGAG 2 cut(s) 1581, 1899
BssT1I CCWWGG 2 cut(s) 2029, 2329
Bst2BI CACGAG 2 cut(s) 1581, 1899
Bst2UI CCWGG 6 cut(s) 145, 512, 576, 651, 750, 996
Bst4CI ACNGT 7 cut(s) 307, 933, 989, 1333, 1404, 1851, 2578
Bst6I CTCTTC 1 cut(s) 122
BstAUI TGTACA 2 cut(s) 1287, 2407
BstC8I GCNNGC 5 cut(s) 756, 1389, 1416, 1440, 2326
BstDEI CTNAG 5 cut(s) 282, 677, 847, 1193, 1515
BstDSI CCRYGG 2 cut(s) 1600, 2029
BstENI CCTNNNNNAGG 1 cut(s) 1812
BstF5I GGATG 7 cut(s) 187, 520, 553, 871, 1055, 1791, 2362
BstFNI CGCG 2 cut(s) 439, 1602
BstH2I RGCGCY 1 cut(s) 761
BstHHI GCGC 4 cut(s) 760, 1183, 1414, 2398
BstMAI GTCTC 5 cut(s) 775, 1086, 1299, 1513, 2028
BstMCI CGRYCG 1 cut(s) 443
BstMWI GCNNNNNNNGC 5 cut(s) 954, 1346, 1605, 1721, 2441
BstNI CCWGG 6 cut(s) 145, 512, 576, 651, 750, 996
BstNSI RCATGY 2 cut(s) 1807, 2086
BstSCI CCNGG 8 cut(s) 143, 241, 510, 574, 649, 748, 911, 994
BstSFI CTRYAG 4 cut(s) 70, 558, 670, 2296
BstUI CGCG 2 cut(s) 439, 1602
BstV1I GCAGC 2 cut(s) 1154, 1574
BstV2I GAAGAC 1 cut(s) 897
BstX2I RGATCY 4 cut(s) 1951, 2200, 2416, 2552
BstYI RGATCY 4 cut(s) 1951, 2200, 2416, 2552
Bsu15I ATCGAT 1 cut(s) 1136
BsuRI GGCC 4 cut(s) 754, 876, 1599, 2172
BsuTUI ATCGAT 1 cut(s) 1136
BtgI CCRYGG 2 cut(s) 1600, 2029
BtrI CACGTC 1 cut(s) 2225
BtsCI GGATG 7 cut(s) 187, 520, 553, 871, 1055, 1791, 2362
BtsI GCAGTG 1 cut(s) 1209
BtsIMutI CAGTG 4 cut(s) 312, 938, 1209, 1224
BveI ACCTGC 1 cut(s) 2440
Cac8I GCNNGC 5 cut(s) 756, 1389, 1416, 1440, 2326
CciI TCATGA 1 cut(s) 798
CfoI GCGC 4 cut(s) 760, 1183, 1414, 2398
Cfr10I RCCGGY 1 cut(s) 258
Cfr13I GGNCC 2 cut(s) 752, 1851
Cfr42I CCGCGG 1 cut(s) 1603
ClaI ATCGAT 1 cut(s) 1136
Csp6I GTAC 5 cut(s) 925, 1288, 1424, 2095, 2408
CspAI ACCGGT 1 cut(s) 258
CviQI GTAC 5 cut(s) 925, 1288, 1424, 2095, 2408
DdeI CTNAG 5 cut(s) 282, 677, 847, 1193, 1515
DraI TTTAAA 1 cut(s) 2281
DrdI GACNNNNNNGTC 1 cut(s) 1849
DseDI GACNNNNNNGTC 1 cut(s) 1849
EaeI YGGCCR 2 cut(s) 1597, 2170
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
Ecl136II GAGCTC 1 cut(s) 2311
Eco130I CCWWGG 2 cut(s) 2029, 2329
Eco24I GRGCYC 1 cut(s) 2313
Eco31I GGTCTC 3 cut(s) 775, 1299, 2028
Eco47I GGWCC 1 cut(s) 1851
Eco53kI GAGCTC 1 cut(s) 2311
Eco57I CTGAAG 2 cut(s) 220, 1662
EcoICRI GAGCTC 1 cut(s) 2311
EcoNI CCTNNNNNAGG 1 cut(s) 1812
EcoRII CCWGG 6 cut(s) 143, 510, 574, 649, 748, 994
EcoT14I CCWWGG 2 cut(s) 2029, 2329
EcoT22I ATGCAT 1 cut(s) 697
EcoT38I GRGCYC 1 cut(s) 2313
ErhI CCWWGG 2 cut(s) 2029, 2329
Esp3I CGTCTC 1 cut(s) 1086
FalI AAGNNNNNCTT 6 cut(s) 96, 128, 878, 910, 1701, 1733
FaqI GGGAC 3 cut(s) 161, 674, 895
FauNDI CATATG 1 cut(s) 2074
FbaI TGATCA 1 cut(s) 550
FblI GTMKAC 2 cut(s) 425, 1406
Fnu4HI GCNGC 4 cut(s) 1143, 1341, 1563, 1600
FokI GGATG 7 cut(s) 194, 527, 540, 878, 1062, 1798, 2349
FriOI GRGCYC 1 cut(s) 2313
Fsp4HI GCNGC 4 cut(s) 1143, 1341, 1563, 1600
FspBI CTAG 8 cut(s) 252, 288, 383, 1415, 1796, 1962, 1976, 2019
FspI TGCGCA 1 cut(s) 1182
GlaI GCGC 4 cut(s) 759, 1182, 1413, 2397
GluI GCNGC 4 cut(s) 1143, 1341, 1563, 1600
HaeII RGCGCY 1 cut(s) 761
HaeIII GGCC 4 cut(s) 754, 876, 1599, 2172
HapII CCGG 4 cut(s) 243, 259, 913, 2195
HhaI GCGC 4 cut(s) 760, 1183, 1414, 2398
Hin6I GCGC 4 cut(s) 758, 1181, 1412, 2396
HinP1I GCGC 4 cut(s) 758, 1181, 1412, 2396
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HinfI GANTC 8 cut(s) 270, 839, 1027, 1823, 1906, 1925, 2343, 2492
HpaI GTTAAC 1 cut(s) 457
HpaII CCGG 4 cut(s) 243, 259, 913, 2195
HphI GGTGA 5 cut(s) 13, 203, 604, 1910, 2079
Hpy166II GTNNAC 7 cut(s) 426, 457, 1288, 1290, 1407, 2408, 2506
Hpy188I TCNGA 9 cut(s) 285, 685, 1032, 1518, 2005, 2128, 2191, 2315, 2421
Hpy188III TCNNGA 8 cut(s) 779, 799, 1127, 1547, 1680, 1858, 1929, 2195
Hpy8I GTNNAC 7 cut(s) 426, 457, 1288, 1290, 1407, 2408, 2506
HpyAV CCTTC 7 cut(s) 172, 880, 887, 1229, 1864, 2214, 2314
HpyCH4III ACNGT 7 cut(s) 307, 933, 989, 1333, 1404, 1851, 2578
HpyCH4IV ACGT 2 cut(s) 432, 2224
HpyF10VI GCNNNNNNNGC 5 cut(s) 954, 1346, 1605, 1721, 2441
HpyF3I CTNAG 5 cut(s) 282, 677, 847, 1193, 1515
HpySE526I ACGT 2 cut(s) 432, 2224
HspAI GCGC 4 cut(s) 758, 1181, 1412, 2396
Kpn2I TCCGGA 1 cut(s) 2194
Ksp22I TGATCA 1 cut(s) 550
KspAI GTTAAC 1 cut(s) 457
KspI CCGCGG 1 cut(s) 1603
LmnI GCTCC 2 cut(s) 1501, 2308
Lsp1109I GCAGC 2 cut(s) 1154, 1574
LweI GCATC 4 cut(s) 42, 704, 1154, 2480
MaeI CTAG 8 cut(s) 252, 288, 383, 1415, 1796, 1962, 1976, 2019
MaeII ACGT 2 cut(s) 432, 2224
MaeIII GTNAC 8 cut(s) 191, 636, 832, 917, 983, 1166, 2085, 2578
MfeI CAATTG 1 cut(s) 173
MflI RGATCY 4 cut(s) 1951, 2200, 2416, 2552
MhlI GDGCHC 4 cut(s) 360, 855, 1226, 2313
MlsI TGGCCA 1 cut(s) 2172
MluNI TGGCCA 1 cut(s) 2172
MlyI GAGTC 4 cut(s) 279, 1817, 1919, 2501
MmeI TCCRAC 3 cut(s) 247, 2047, 2136
Mox20I TGGCCA 1 cut(s) 2172
Mph1103I ATGCAT 1 cut(s) 697
MroI TCCGGA 1 cut(s) 2194
MroXI GAANNNNTTC 1 cut(s) 1201
MscI TGGCCA 1 cut(s) 2172
MseI TTAA 9 cut(s) 456, 569, 623, 969, 1119, 1151, 1701, 2216, 2280
MslI CAYNNNNRTG 3 cut(s) 1283, 1447, 2367
Msp20I TGGCCA 1 cut(s) 2172
MspA1I CMGCKG 1 cut(s) 1602
MspI CCGG 4 cut(s) 243, 259, 913, 2195
MspR9I CCNGG 8 cut(s) 145, 243, 512, 576, 651, 750, 913, 996
MunI CAATTG 1 cut(s) 173
Mva1269I GAATGC 1 cut(s) 1330
MvaI CCWGG 6 cut(s) 145, 512, 576, 651, 750, 996
MvnI CGCG 2 cut(s) 439, 1602
MwoI GCNNNNNNNGC 5 cut(s) 954, 1346, 1605, 1721, 2441
NciI CCSGG 2 cut(s) 243, 913
NcoI CCATGG 1 cut(s) 2029
NdeI CATATG 1 cut(s) 2074
NheI GCTAGC 1 cut(s) 1414
NlaIV GGNNCC 3 cut(s) 940, 1742, 2202
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 7 cut(s) 191, 636, 832, 917, 983, 1166, 2085
NsbI TGCGCA 1 cut(s) 1182
NsiI ATGCAT 1 cut(s) 697
NspI RCATGY 2 cut(s) 1807, 2086
PagI TCATGA 1 cut(s) 798
PciI ACATGT 1 cut(s) 2082
PctI GAATGC 1 cut(s) 1330
PdmI GAANNNNTTC 1 cut(s) 1201
PfeI GAWTC 4 cut(s) 839, 1027, 1906, 2343
PfoI TCCNGGA 1 cut(s) 510
PinAI ACCGGT 1 cut(s) 258
PkrI GCNGC 4 cut(s) 1144, 1342, 1564, 1601
PleI GAGTC 4 cut(s) 278, 1817, 1919, 2500
PpsI GAGTC 4 cut(s) 278, 1817, 1919, 2500
PscI ACATGT 1 cut(s) 2082
PsiI TTATAA 2 cut(s) 1103, 1623
Psp124BI GAGCTC 1 cut(s) 2313
Psp6I CCWGG 6 cut(s) 143, 510, 574, 649, 748, 994
PspGI CCWGG 6 cut(s) 143, 510, 574, 649, 748, 994
PspN4I GGNNCC 3 cut(s) 940, 1742, 2202
PspPI GGNCC 2 cut(s) 752, 1851
PstI CTGCAG 1 cut(s) 2300
PsuI RGATCY 4 cut(s) 1951, 2200, 2416, 2552
RsaI GTAC 5 cut(s) 926, 1289, 1425, 2096, 2409
RsaNI GTAC 5 cut(s) 925, 1288, 1424, 2095, 2408
RseI CAYNNNNRTG 3 cut(s) 1283, 1447, 2367
SacI GAGCTC 1 cut(s) 2313
SacII CCGCGG 1 cut(s) 1603
SaqAI TTAA 9 cut(s) 456, 569, 623, 969, 1119, 1151, 1701, 2216, 2280
SatI GCNGC 4 cut(s) 1143, 1341, 1563, 1600
Sau96I GGNCC 2 cut(s) 752, 1851
SchI GAGTC 4 cut(s) 279, 1817, 1919, 2501
ScrFI CCNGG 8 cut(s) 145, 243, 512, 576, 651, 750, 913, 996
SduI GDGCHC 4 cut(s) 360, 855, 1226, 2313
SfaNI GCATC 4 cut(s) 42, 704, 1154, 2480
SfcI CTRYAG 4 cut(s) 70, 558, 670, 2296
Sfr303I CCGCGG 1 cut(s) 1603
SgrBI CCGCGG 1 cut(s) 1603
SinI GGWCC 1 cut(s) 1851
SmiMI CAYNNNNRTG 3 cut(s) 1283, 1447, 2367
SmlI CTYRAG 3 cut(s) 28, 1505, 1927
SmoI CTYRAG 3 cut(s) 28, 1505, 1927
SpeI ACTAGT 2 cut(s) 382, 1961
SsiI CCGC 4 cut(s) 439, 1340, 1600, 1602
SspI AATATT 1 cut(s) 2025
SspMI CTAG 8 cut(s) 252, 288, 383, 1415, 1796, 1962, 1976, 2019
SstI GAGCTC 1 cut(s) 2313
StyD4I CCNGG 8 cut(s) 143, 241, 510, 574, 649, 748, 911, 994
StyI CCWWGG 2 cut(s) 2029, 2329
TaaI ACNGT 7 cut(s) 307, 933, 989, 1333, 1404, 1851, 2578
TaiI ACGT 2 cut(s) 435, 2227
TaqI TCGA 3 cut(s) 1063, 1136, 2596
TaqII GACCGA 1 cut(s) 2442
TatI WGTACW 2 cut(s) 1287, 2407
TauI GCSGC 2 cut(s) 1343, 1602
TfiI GAWTC 4 cut(s) 839, 1027, 1906, 2343
Tru1I TTAA 9 cut(s) 456, 569, 623, 969, 1119, 1151, 1701, 2216, 2280
Tru9I TTAA 9 cut(s) 456, 569, 623, 969, 1119, 1151, 1701, 2216, 2280
TscAI CASTG 4 cut(s) 312, 938, 1216, 1231
TseFI GTSAC 7 cut(s) 191, 636, 832, 917, 983, 1166, 2085
TseI GCWGC 2 cut(s) 1142, 1562
Tsp45I GTSAC 7 cut(s) 191, 636, 832, 917, 983, 1166, 2085
TspGWI ACGGA 1 cut(s) 942
TspRI CASTG 4 cut(s) 312, 938, 1216, 1231
VpaK11BI GGWCC 1 cut(s) 1851
XagI CCTNNNNNAGG 1 cut(s) 1812
XapI RAATTY 6 cut(s) 247, 496, 1697, 1998, 2287, 2515
XceI RCATGY 2 cut(s) 1807, 2086
XcmI CCANNNNNNNNNTGG 1 cut(s) 2180
XmiI GTMKAC 2 cut(s) 425, 1406
XmnI GAANNNNTTC 1 cut(s) 1201
XspI CTAG 8 cut(s) 252, 288, 383, 1415, 1796, 1962, 1976, 2019
Zsp2I ATGCAT 1 cut(s) 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.