RLG00000023753

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000112
Physical Location & Seq
Reverse (-)
27815397 .. 27818814
3418 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023753

Sequence Viewer

Length: 2250 bp
ATGTGGAAGACAAAGACACTGAAAATTTGCAGGGACACCATCAGATGGAATGAACAACAATTGAAGGATGTTGATGGTGAAGTTTTGGTGTCAAAAGAAAACAACTTTGAGTTGGGTTTCTTCAGCCCCGGCGATTCTAGCCACCGGTACGTTGGTGTTTGGTATTCTCAATCTAAAATATCTGCAAAAACAGTGGTGTGGATCGCAAACAGAGACAGCCCCATCAATGATACCTCTGGGGTTCTCAAAATGAACAGGTATGGAGAATTAGCTCTTTATGCTTATAACATGGAGAACATTCCTATATGGTCCACAAATGCGTCACAGTCGGTCCAAAACACAGACACCTTATATAGATATGTACAGCTTTTAGATACTGGAAACCTAGTCGTGTTCCGGGATGGTAGAAATGGAAGCTTGATTTTATGGCAAAGTTTTGATTATCCTACAGATACTCTAATCCCAGGTATGAAAGCTGGGTTGAATTGGAAAAAAGGGCTAGATTGGGTTTTAACATCTTGGAAGTCACCAGATGACCCTGGAAAGGGGGACTATGCGTTTAGGCTCGATCCGAATCACAATGCTACTCCCCAATATTTTGTCTACAGGGGTTTCACTAAGTATTGGCGAACCTATGCAGTGCCATGGCCAAATTTTGTTAGTAATGCCGACGAAATGTATATTACCCTGCCTTATAAGAGCAATGCTATTTCGATACTAAAGTTAAAGGATTCCGGGTTACTTCAGCACCTAATATGGAATGATGCTGACCATCAATGGGTGGAAATGTGGTCTTCGCCGAAATACCCTTGTGACTGGTATGGACATTGTGGTGCCAACAGCAAATGTAGCCCTGACAATATTAATTTATTTGAGTGTGAGTGTATTCCAGGGTATGAACCAAAATCTGTAGATGGTTGGAATCGGAGAGATGGAACAGATGGATGTGTGAGTAAACGAGTAAGAGTGTCAAAGTGTGGGAATGGAGAAGGGTTTGTGAAGGTGGCAAGGGTAAAAGATCCAGACACATCGAATGTAGCACGGCTGAGAACAAGTATGAGTGCCAAAGAGTGTGAGCAGGAGTGCTTTAAAAATTGTTCTTGCACGGCGTATATGAGCATTACAATGGAAAGGGTAGTCAATTGCTTGACATGGTATGATGACTTGATGGACATGTTAGTTTACACAGAGGTCGGACGAGATCTATATGTTCGTGTGGATAAAATCTCATTAGATGAAATGATTGAGAGAATATTTTTCTTGTTTCTCATTTGTTTTACTTTTTTTTTTTGTTTTTTGAACTTTATGATGGCAAAGACTATTTGGATGCTGAGGAACTTGAGGAAAGGCGAATTACCAAACGAACAAAAAGTTGCTATAAAAAGATTGTCTAGAACTTCAGGACAGGGAATCGAAGAATTCAAAAACGAAGTTGCACTAATAGCCAGACTTCAGCACAGGAATCTTGTGAAACTTTTAGGCTGTTGTGTAAAGGGAGAAGAAAGGATATTGGTTCTGGAATACATGCCGAACAAAAGCTTGGACTCCTTTCTTTTTGATAACACAAGGAGGTCACTTTTAAATTGGGAAAAGCGTTTTGAAATTATCAATGGGGTTGCTCGTGGGGTTCTATATCTTCATCGGGACTCAAGATTGAGGGTTATCCATAGGGATCTGAAAACCAGTAACGTCCTTTTAGATGCCGAGTTAGACCCAAAAATTTCAGATTTCGGCATGGCAAGAATGTTTCAAGGGGACCAACTCCTGGAAAAGACAAACAGAGTTGTCGGAACATATGGCTACATGTCACCAGAATATGCAATATTTGGGAGATTTTCTACGAAATCGGATGTGTTTAGTTTTGGGATCATACTATTGGAGATTGTTAGTGGCAAGAGAAACAATGGTTCTTATGAGATGCATTATTCCATGAACTTGATAGGACATGTATGGCAACTTTGGGTAGAAGACAAAGTCCTCGAAATTGTGGATTCATCACTGGAGATTTATCAGCTTGATGAAGTCTTGAGATGCATTCAAGTGGGGCTTTTGTGCGTGCAAGAAAATTTGAAGGACCGACCAGCCATGTCAGCCGTCGTGTTCATGTTGAGTGGTGAAACATCTCTTCCATCTCCTAAGCAGCCTGCATTTGTTTTCAGAACAAGTTCCAGCATTGCTGATGATCCCCTCATTCCAAATGAATCATATTCTAGTAATAGTTTGACAATAACTAACATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

750

Amino Acids

86.26

Weight (kDa)

6.81

Isoelectric Point (pI)

39.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 64 - 175 4.5e-33 D-mannose binding lectin
S_locus_glycop PF00954 218 - 303 8.4e-16 S-locus glycoprotein domain
PAN_2 PF08276 326 - 392 2.5e-15 PAN-like domain
PK_Tyr_Ser-Thr PF07714 453 - 700 9e-44 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 454 - 634 5.6e-38 Protein kinase domain
DUF3403 PF11883 706 - 749 1.2e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 285, 696
AccB1I GGYRCC 1 cut(s) 833
AccB7I CCANNNNNTGG 2 cut(s) 45, 1765
AccI GTMKAC 1 cut(s) 603
AclWI GGATC 6 cut(s) 209, 563, 1013, 1680, 1874, 2177
AcoI YGGCCR 1 cut(s) 647
AcsI RAATTY 5 cut(s) 24, 652, 1418, 1719, 2065
AcuI CTGAAG 4 cut(s) 106, 728, 1383, 1436
AfaI GTAC 2 cut(s) 149, 363
AfiI CCNNNNNNNGG 5 cut(s) 45, 544, 545, 778, 1765
AflIII ACRYGT 3 cut(s) 1173, 1803, 1945
AgeI ACCGGT 1 cut(s) 144
AgsI TTSAA 8 cut(s) 64, 484, 1300, 1423, 1601, 1751, 2039, 2071
AjnI CCWGG 4 cut(s) 463, 538, 889, 1764
AjuI GAANNNNNNNTTGG 2 cut(s) 1523, 1555
AluBI AGCT 7 cut(s) 272, 367, 417, 476, 1539, 2014, 2243
AluI AGCT 7 cut(s) 272, 367, 417, 476, 1539, 2014, 2243
Alw26I GTCTC 1 cut(s) 207
AlwI GGATC 6 cut(s) 209, 563, 1013, 1680, 1874, 2177
AoxI GGCC 1 cut(s) 647
ApeKI GCWGC 1 cut(s) 2140
ApoI RAATTY 5 cut(s) 24, 652, 1418, 1719, 2065
AseI ATTAAT 1 cut(s) 864
AsiGI ACCGGT 1 cut(s) 144
Asp700I GAANNNNTTC 1 cut(s) 2164
AspS9I GGNCC 4 cut(s) 309, 331, 1756, 2074
AsuC2I CCSGG 3 cut(s) 129, 398, 736
AsuHPI GGTGA 4 cut(s) 89, 519, 1800, 2126
AvaII GGWCC 4 cut(s) 309, 331, 1756, 2074
BaeI ACNNNNGTAYC 4 cut(s) 139, 172, 707, 740
BalI TGGCCA 1 cut(s) 649
BanI GGYRCC 1 cut(s) 833
BauI CACGAG 1 cut(s) 1620
BbsI GAAGAC 3 cut(s) 14, 786, 1974
BbvCI CCTCAGC 1 cut(s) 1331
BbvI GCAGC 1 cut(s) 2152
BceAI ACGGC 3 cut(s) 1058, 1122, 2078
BciT130I CCWGG 4 cut(s) 465, 540, 891, 1766
BcnI CCSGG 3 cut(s) 129, 398, 736
BcoDI GTCTC 1 cut(s) 207
BfaI CTAG 5 cut(s) 138, 386, 500, 1392, 2211
BfmI CTRYAG 3 cut(s) 447, 604, 909
BglII AGATCT 1 cut(s) 1201
BisI GCNGC 1 cut(s) 2141
BlsI GCNGC 1 cut(s) 2142
Bme1390I CCNGG 7 cut(s) 129, 398, 465, 540, 736, 891, 1766
Bme18I GGWCC 4 cut(s) 309, 331, 1756, 2074
BmgT120I GGNCC 4 cut(s) 309, 331, 1756, 2074
BmiI GGNNCC 2 cut(s) 835, 1757
BmrFI CCNGG 7 cut(s) 129, 398, 465, 540, 736, 891, 1766
BmsI GCATC 5 cut(s) 754, 1317, 1690, 1908, 2021
BpiI GAAGAC 3 cut(s) 14, 786, 1974
BpmI CTGGAG 1 cut(s) 2021
Bpu10I CCTNAGC 2 cut(s) 1331, 2136
BpuEI CTTGAG 3 cut(s) 1360, 1633, 2047
BpuMI CCSGG 3 cut(s) 129, 398, 736
BsaBI GATNNNNATC 1 cut(s) 573
BsaJI CCNNGG 5 cut(s) 127, 463, 538, 644, 890
BsaWI WCCGGW 1 cut(s) 144
Bsc4I CCNNNNNNNGG 5 cut(s) 45, 544, 545, 778, 1765
Bse118I RCCGGY 1 cut(s) 144
Bse1I ACTGG 4 cut(s) 382, 821, 1683, 2004
Bse3DI GCAATG 2 cut(s) 709, 2172
Bse8I GATNNNNATC 1 cut(s) 573
BseBI CCWGG 4 cut(s) 465, 540, 891, 1766
BseDI CCNNGG 5 cut(s) 127, 463, 538, 644, 890
BseGI GGATG 5 cut(s) 73, 406, 950, 1332, 1855
BseJI GATNNNNATC 1 cut(s) 573
BseLI CCNNNNNNNGG 5 cut(s) 45, 544, 545, 778, 1765
BseMI GCAATG 2 cut(s) 709, 2172
BseMII CTCAG 2 cut(s) 1037, 1322
BseNI ACTGG 4 cut(s) 382, 821, 1683, 2004
BseXI GCAGC 1 cut(s) 2152
BseYI CCCAGC 1 cut(s) 476
BshFI GGCC 1 cut(s) 649
BshNI GGYRCC 1 cut(s) 833
BshTI ACCGGT 1 cut(s) 144
BsiSI CCGG 4 cut(s) 129, 145, 397, 735
BslFI GGGAC 4 cut(s) 47, 563, 1658, 1769
BslI CCNNNNNNNGG 5 cut(s) 45, 544, 545, 778, 1765
BsmAI GTCTC 1 cut(s) 207
BsmFI GGGAC 4 cut(s) 47, 563, 1658, 1769
BsmI GAATGC 1 cut(s) 2034
BsnI GGCC 1 cut(s) 649
Bsp1407I TGTACA 1 cut(s) 361
Bsp143I GATC 7 cut(s) 201, 568, 1018, 1201, 1672, 1866, 2182
Bsp19I CCATGG 1 cut(s) 644
BspANI GGCC 1 cut(s) 649
BspCNI CTCAG 2 cut(s) 1038, 1323
BspLI GGNNCC 2 cut(s) 835, 1757
BspPI GGATC 6 cut(s) 209, 563, 1013, 1680, 1874, 2177
BspT107I GGYRCC 1 cut(s) 833
BsrDI GCAATG 2 cut(s) 709, 2172
BsrFI RCCGGY 1 cut(s) 144
BsrGI TGTACA 1 cut(s) 361
BsrI ACTGG 4 cut(s) 382, 821, 1683, 2004
BssAI RCCGGY 1 cut(s) 144
BssECI CCNNGG 5 cut(s) 127, 463, 538, 644, 890
BssMI GATC 7 cut(s) 201, 568, 1018, 1201, 1672, 1866, 2182
BssSI CACGAG 1 cut(s) 1620
BssT1I CCWWGG 1 cut(s) 644
Bst2BI CACGAG 1 cut(s) 1620
Bst2UI CCWGG 4 cut(s) 465, 540, 891, 1766
Bst4CI ACNGT 2 cut(s) 193, 327
Bst6I CTCTTC 1 cut(s) 2130
BstAUI TGTACA 1 cut(s) 361
BstC8I GCNNGC 2 cut(s) 2057, 2145
BstDEI CTNAG 4 cut(s) 618, 1046, 1331, 2136
BstDSI CCRYGG 1 cut(s) 644
BstF5I GGATG 5 cut(s) 73, 406, 950, 1332, 1855
BstKTI GATC 7 cut(s) 204, 571, 1021, 1204, 1675, 1869, 2185
BstMAI GTCTC 1 cut(s) 207
BstMBI GATC 7 cut(s) 201, 568, 1018, 1201, 1672, 1866, 2182
BstMWI GCNNNNNNNGC 5 cut(s) 138, 278, 849, 1442, 2090
BstNI CCWGG 4 cut(s) 465, 540, 891, 1766
BstNSI RCATGY 4 cut(s) 1177, 1528, 1807, 1949
BstSCI CCNGG 7 cut(s) 127, 396, 463, 538, 734, 889, 1764
BstSFI CTRYAG 3 cut(s) 447, 604, 909
BstV1I GCAGC 1 cut(s) 2152
BstV2I GAAGAC 3 cut(s) 14, 786, 1974
BstX2I RGATCY 3 cut(s) 1018, 1201, 1672
BstYI RGATCY 3 cut(s) 1018, 1201, 1672
BsuRI GGCC 1 cut(s) 649
BtgI CCRYGG 1 cut(s) 644
BtsCI GGATG 5 cut(s) 73, 406, 950, 1332, 1855
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 4 cut(s) 17, 198, 645, 1997
Cac8I GCNNGC 2 cut(s) 2057, 2145
Cfr10I RCCGGY 1 cut(s) 144
Cfr13I GGNCC 4 cut(s) 309, 331, 1756, 2074
CseI GACGC 1 cut(s) 309
Csp6I GTAC 2 cut(s) 148, 362
CspAI ACCGGT 1 cut(s) 144
CspCI CAANNNNNGTGG 2 cut(s) 174, 209
CviQI GTAC 2 cut(s) 148, 362
DdeI CTNAG 4 cut(s) 618, 1046, 1331, 2136
DpnI GATC 7 cut(s) 203, 570, 1020, 1203, 1674, 1868, 2184
DpnII GATC 7 cut(s) 201, 568, 1018, 1201, 1672, 1866, 2182
DraI TTTAAA 2 cut(s) 1090, 1581
EaeI YGGCCR 1 cut(s) 647
Eam1104I CTCTTC 1 cut(s) 2130
EarI CTCTTC 1 cut(s) 2130
Eco130I CCWWGG 1 cut(s) 644
Eco47I GGWCC 4 cut(s) 309, 331, 1756, 2074
Eco57I CTGAAG 4 cut(s) 106, 728, 1383, 1436
EcoRI GAATTC 1 cut(s) 1418
EcoRII CCWGG 4 cut(s) 463, 538, 889, 1764
EcoT14I CCWWGG 1 cut(s) 644
EcoT22I ATGCAT 2 cut(s) 1923, 2036
ErhI CCWWGG 1 cut(s) 644
FalI AAGNNNNNCTT 2 cut(s) 2031, 2063
FaqI GGGAC 4 cut(s) 47, 563, 1658, 1769
FauNDI CATATG 1 cut(s) 1795
FblI GTMKAC 1 cut(s) 603
Fnu4HI GCNGC 1 cut(s) 2141
FokI GGATG 5 cut(s) 80, 413, 957, 1339, 1862
Fsp4HI GCNGC 1 cut(s) 2141
FspBI CTAG 5 cut(s) 138, 386, 500, 1392, 2211
GluI GCNGC 1 cut(s) 2141
GsaI CCCAGC 1 cut(s) 480
GsuI CTGGAG 1 cut(s) 2021
HaeIII GGCC 1 cut(s) 649
HapII CCGG 4 cut(s) 129, 145, 397, 735
HgaI GACGC 1 cut(s) 309
HindIII AAGCTT 2 cut(s) 415, 1537
HpaII CCGG 4 cut(s) 129, 145, 397, 735
HphI GGTGA 4 cut(s) 89, 519, 1800, 2126
Hpy166II GTNNAC 4 cut(s) 312, 604, 956, 1183
Hpy188I TCNGA 9 cut(s) 44, 573, 927, 1196, 1677, 1726, 1790, 1849, 2159
Hpy188III TCNNGA 7 cut(s) 1022, 1392, 1401, 1517, 1643, 1650, 2026
Hpy8I GTNNAC 4 cut(s) 312, 604, 956, 1183
Hpy99I CGWCG 2 cut(s) 674, 2099
HpyAV CCTTC 4 cut(s) 58, 983, 994, 2065
HpyCH4III ACNGT 2 cut(s) 193, 327
HpyCH4IV ACGT 2 cut(s) 150, 1689
HpyF10VI GCNNNNNNNGC 5 cut(s) 138, 278, 849, 1442, 2090
HpyF3I CTNAG 4 cut(s) 618, 1046, 1331, 2136
HpySE526I ACGT 2 cut(s) 150, 1689
Kzo9I GATC 7 cut(s) 201, 568, 1018, 1201, 1672, 1866, 2182
Lsp1109I GCAGC 1 cut(s) 2152
LweI GCATC 5 cut(s) 754, 1317, 1690, 1908, 2021
MaeI CTAG 5 cut(s) 138, 386, 500, 1392, 2211
MaeII ACGT 2 cut(s) 150, 1689
MaeIII GTNAC 7 cut(s) 321, 525, 738, 812, 1572, 1685, 1806
MalI GATC 7 cut(s) 203, 570, 1020, 1203, 1674, 1868, 2184
MboI GATC 7 cut(s) 201, 568, 1018, 1201, 1672, 1866, 2182
MboII GAAGA 8 cut(s) 19, 112, 786, 1427, 1511, 1628, 1979, 2117
MfeI CAATTG 2 cut(s) 59, 1141
MflI RGATCY 3 cut(s) 1018, 1201, 1672
MlsI TGGCCA 1 cut(s) 649
MluNI TGGCCA 1 cut(s) 649
MlyI GAGTC 2 cut(s) 1538, 1640
MmeI TCCRAC 3 cut(s) 899, 1174, 1768
MnlI CCTC 8 cut(s) 244, 1183, 1326, 1335, 1563, 1650, 1988, 2198
Mox20I TGGCCA 1 cut(s) 649
Mph1103I ATGCAT 2 cut(s) 1923, 2036
MroXI GAANNNNTTC 1 cut(s) 2164
MscI TGGCCA 1 cut(s) 649
MseI TTAA 5 cut(s) 512, 725, 864, 1089, 1580
MslI CAYNNNNRTG 3 cut(s) 831, 1124, 2039
Msp20I TGGCCA 1 cut(s) 649
MspI CCGG 4 cut(s) 129, 145, 397, 735
MspR9I CCNGG 7 cut(s) 129, 398, 465, 540, 736, 891, 1766
MunI CAATTG 2 cut(s) 59, 1141
Mva1269I GAATGC 1 cut(s) 2034
MvaI CCWGG 4 cut(s) 465, 540, 891, 1766
MwoI GCNNNNNNNGC 5 cut(s) 138, 278, 849, 1442, 2090
NciI CCSGG 3 cut(s) 129, 398, 736
NcoI CCATGG 1 cut(s) 644
NdeI CATATG 1 cut(s) 1795
NdeII GATC 7 cut(s) 201, 568, 1018, 1201, 1672, 1866, 2182
NlaIV GGNNCC 2 cut(s) 835, 1757
NmeAIII GCCGAG 1 cut(s) 1729
NmuCI GTSAC 5 cut(s) 321, 525, 812, 1572, 1806
NsiI ATGCAT 2 cut(s) 1923, 2036
NspI RCATGY 4 cut(s) 1177, 1528, 1807, 1949
PciI ACATGT 3 cut(s) 1173, 1803, 1945
PctI GAATGC 1 cut(s) 2034
PdmI GAANNNNTTC 1 cut(s) 2164
PfeI GAWTC 8 cut(s) 134, 574, 731, 922, 1410, 1462, 1991, 2201
PflFI GACNNNGTC 1 cut(s) 1973
PflMI CCANNNNNTGG 2 cut(s) 45, 1765
PfoI TCCNGGA 2 cut(s) 396, 1764
PinAI ACCGGT 1 cut(s) 144
PkrI GCNGC 1 cut(s) 2142
PleI GAGTC 2 cut(s) 1538, 1640
PpsI GAGTC 2 cut(s) 1538, 1640
PscI ACATGT 3 cut(s) 1173, 1803, 1945
PshBI ATTAAT 1 cut(s) 864
PsiI TTATAA 2 cut(s) 285, 696
Psp6I CCWGG 4 cut(s) 463, 538, 889, 1764
PspFI CCCAGC 1 cut(s) 476
PspGI CCWGG 4 cut(s) 463, 538, 889, 1764
PspN4I GGNNCC 2 cut(s) 835, 1757
PspPI GGNCC 4 cut(s) 309, 331, 1756, 2074
PsuI RGATCY 3 cut(s) 1018, 1201, 1672
PsyI GACNNNGTC 1 cut(s) 1973
RsaI GTAC 2 cut(s) 149, 363
RsaNI GTAC 2 cut(s) 148, 362
RseI CAYNNNNRTG 3 cut(s) 831, 1124, 2039
SaqAI TTAA 5 cut(s) 512, 725, 864, 1089, 1580
SatI GCNGC 1 cut(s) 2141
Sau3AI GATC 7 cut(s) 201, 568, 1018, 1201, 1672, 1866, 2182
Sau96I GGNCC 4 cut(s) 309, 331, 1756, 2074
SchI GAGTC 2 cut(s) 1538, 1640
ScrFI CCNGG 7 cut(s) 129, 398, 465, 540, 736, 891, 1766
SfaNI GCATC 5 cut(s) 754, 1317, 1690, 1908, 2021
SfcI CTRYAG 3 cut(s) 447, 604, 909
SinI GGWCC 4 cut(s) 309, 331, 1756, 2074
SmiMI CAYNNNNRTG 3 cut(s) 831, 1124, 2039
SmlI CTYRAG 3 cut(s) 1339, 1648, 2026
SmoI CTYRAG 3 cut(s) 1339, 1648, 2026
SspI AATATT 4 cut(s) 596, 862, 1254, 1824
SspMI CTAG 5 cut(s) 138, 386, 500, 1392, 2211
StyD4I CCNGG 7 cut(s) 127, 396, 463, 538, 734, 889, 1764
StyI CCWWGG 1 cut(s) 644
TaaI ACNGT 2 cut(s) 193, 327
TaiI ACGT 2 cut(s) 153, 1692
TaqI TCGA 6 cut(s) 567, 713, 1031, 1413, 1980, 2245
TaqII GACCGA 2 cut(s) 319, 2091
TatI WGTACW 1 cut(s) 361
TfiI GAWTC 8 cut(s) 134, 574, 731, 922, 1410, 1462, 1991, 2201
Tru1I TTAA 5 cut(s) 512, 725, 864, 1089, 1580
Tru9I TTAA 5 cut(s) 512, 725, 864, 1089, 1580
TscAI CASTG 4 cut(s) 24, 198, 645, 2004
TseFI GTSAC 5 cut(s) 321, 525, 812, 1572, 1806
TseI GCWGC 1 cut(s) 2140
Tsp45I GTSAC 5 cut(s) 321, 525, 812, 1572, 1806
TspRI CASTG 4 cut(s) 24, 198, 645, 2004
Tth111I GACNNNGTC 1 cut(s) 1973
Van91I CCANNNNNTGG 2 cut(s) 45, 1765
VpaK11BI GGWCC 4 cut(s) 309, 331, 1756, 2074
VspI ATTAAT 1 cut(s) 864
XapI RAATTY 5 cut(s) 24, 652, 1418, 1719, 2065
XbaI TCTAGA 1 cut(s) 1391
XceI RCATGY 4 cut(s) 1177, 1528, 1807, 1949
XcmI CCANNNNNNNNNTGG 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 603
XmnI GAANNNNTTC 1 cut(s) 2164
XspI CTAG 5 cut(s) 138, 386, 500, 1392, 2211
Zsp2I ATGCAT 2 cut(s) 1923, 2036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.