MD05G1332000.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000112
Physical Location & Seq
Reverse (-)
45569985 .. 45573637
3653 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1332000.v1.1.491

Sequence Viewer

Length: 2550 bp
ATGACTGTCAACATCCAAAGTCTCTTTGACTCGAGCCATGCTTGCCAGTTGCCGGGTAAACTAGTCCACTCAAATAAGCAGCAGAAATTCATCGTAGATGCGAAATCCCAAATCCCCAGAAGAAAAATGTTATTCTTCAAGCCTCTGTTTCTGTTCTTCCTCCTCCTTATGCCATTCTGCACATCCATTGACACCATAGCACTCAACCAACAGCTCAAAGACGGCGACACTCTAGTCTCCAAAGACAGAAAATTTGAGCTTGGATTCTTCAGCCCCGACAACTCCAGTAGTTCCACGTACGTCGGTATCTGGTATGTAGATCAACCTCAAAAAACAGTTGTTTGGGTGGCAAACAGAGACACCCCCGTCAACGATAGTTCCGGAGTTCTAGCAATTAACAGGTACGGAAATCTGGTTCTCTCTGCTGCTGGGAATATTTTGATTTGGTCAACGAATGTGTCTGTCCATGTCCAAACCAGAAGCACTTTTGTAGCTCAGCTTTTAGATTCTGGAAATTTAGTGCTTTTCCAGGAAAATAAAAGTGAGGGCTTTATTTGGCAGAGTTTTGATTACCCAACTGACACTTTACTTCCAGGAATGAAAATTGGGTTCAATTGGAAAAGTGGGTTGGAATTGAAATTGACATCTTGGATTTCCGAAAACAACCCTGGAACCGGGGACTACAGTTTCAGGATAGTTTCCAATTCAACCGCCACCCCTCTAATTGTTGTGTTTAAGGGTTTGAGCAAGTATTGGAGAGCTGATCCCGGGGTGTGGCCGAATTTTGTAAGCAATGATGAGGAAATGTACGATTATTTTACGCCGAGGGAAAACGGTTCTGTAGCACGAGCGGTGTTGAGGGATTATGGATTGTTTCAGCAGCTGAAATGGAGTGATGCTGATGATCAGTGGGAGGAAACTTTCGCTGCACCAAAATATCGGTGTGACAAGTACGGACAGTGTGGTGCTAACAGCATATGTAGCCCTGACAATGTTAATCTGTTTGAGTGTGAGTGTTTACCTGGGTATGAACCGAAATCTGTAAATAATTGGAATCAGAGAAATGGGTCGGATGGGTGCGTGAGTAAGCGAATTGGGGGCTTGAAATGTGGGAATGGGGACGGGTTCGTGACGTTTCAAAGAGTGAAAGATCCAGATGCATCGATAGAAGCACAGTTGGTTAAAAGCATGAGTGATAAAGAATGTGAGCAAGCGTGCTTAAGCAATTGTTCATGCTCTGCGTATATGAGCATAGGGTCGGAAGGGCGGATTGATTGCATGACATGGTACGATGATTTGATGGACATTTTAGTGCGCACAGAGTTGGGAGGAGACTTATATGTTCGCGTGGACAAAATCGAATTAGGTAAGCATACCAGCAAATCAAAAGTTTTTTTGAGACGGAGGGGCGCTCTGGTTATTCCAATAGTTTCGGTACTGGTGGCATTGGTACTAATCATTGTGTTTGCCTGCTGGCGGCATAAGAAAAAGAGCAAAACAAAAGACTACGTGGAGGTAGATGAACTTGAGGAAACTAGGAGACACCCTGAGTTGCAATATTTCGACCTGAGCACCATACTAGTGGCCACAGACAACTTCTCGCTCGTTAATAAACTTGGCCAAGGGGGTTTTGGCACTGTTTATAAGGGCCAGGTACCAAATGGTCAGATTATTGCTGTGAAAAGATTGTCAAGAACTTCAGGACAAGGGGTTAAAGAATTCAAAAATGAAGTTGCGCTGATAGCGAGACTTCAACACAGGAATCTGGTGAAACTTTTAGGCTGTTGTGTAAAGGGAGAAGAAAGGATATTAGTCCTGGAACACTTGCCAAACAAAAGTTTGGACTACTTTCTTTTCGATCACAGAAGAAGGTCCTTGTTGGAGTGGGATAAGCGTTTTGAAATCATCAACGGGGTTGCTCGTGGGATTCTGTATCTTCACCAGGACTCAAGAGTTAGGATTATCCATAGGGATCTAAAAACCAGCAATATTCTTCTAGATGCTGAGATGAACCCGAAAATTTCAGATTTCGGAATGGCAAGAATATTCCAGGGGGACCAACTGCAGGACAAGACCAACAGAATTGTTGGAACATATGGCTATATGTCACCAGAGTATGCAGTGTTTGGGAGATTTTCAACAAAGTCGGATGTCTTTAGTTTTGGGATCATTTTATTGGAGATTGTAAGCAGCAAGAAAAACAACGGTTTATATCAGGAGGATCATTCCATGAACTTGATAGGACATGTTTGGCAGCTGTGGAGAGAAGACAGAGCCTTAGAAATTGTGGATTCGTCATTAGAGTCATATCAGTCCGAGGAAGTCGTGAGATGCATTCAGGTTGGCCTTTTGTGTGTGCAAGAAGATCCAGAGGACCGCCCAACCATGGCAGCTGTTGTTTTCATGTTGAGTGGCGAAGCATCTCCTCCAGGTCCTAAGCAGCCTGCGTTTGTATTCAGAAGCAAACCCTGCAGTAATGCTGATCCATCAGTTTCGAAGGAATCATGTTCTATAAACGACTTGACAATAACTAAATTTCAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

850

Amino Acids

95.89

Weight (kDa)

7.14

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 112 - 217 1.8e-37 D-mannose binding lectin
S_locus_glycop PF00954 260 - 347 2.6e-16 S-locus glycoprotein domain
PAN_2 PF08276 370 - 436 1.6e-15 PAN-like domain
Pkinase PF00069 534 - 799 6.1e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 535 - 800 3.1e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1644
AasI GACNNNNNNGTC 2 cut(s) 233, 365
Acc16I TGCGCA 1 cut(s) 1316
Acc65I GGTACC 1 cut(s) 1654
AccB1I GGYRCC 1 cut(s) 1654
AccBSI CCGCTC 1 cut(s) 851
AccII CGCG 1 cut(s) 1347
AccIII TCCGGA 1 cut(s) 380
AciI CCGC 5 cut(s) 711, 851, 1267, 1477, 2377
AclWI GGATC 7 cut(s) 758, 1145, 1980, 2174, 2229, 2360, 2477
AcoI YGGCCR 3 cut(s) 776, 1584, 1618
AcsI RAATTY 7 cut(s) 86, 251, 514, 781, 1718, 2019, 2534
AcuI CTGAAG 2 cut(s) 253, 1683
AfaI GTAC 8 cut(s) 299, 404, 809, 953, 1289, 1437, 1452, 1656
AfiI CCNNNNNNNGG 6 cut(s) 52, 674, 774, 1476, 2065, 2386
AflII CTTAAG 1 cut(s) 1219
AflIII ACRYGT 1 cut(s) 2245
AhlI ACTAGT 2 cut(s) 61, 1579
AjnI CCWGG 9 cut(s) 528, 592, 667, 1021, 1650, 1815, 1941, 2049, 2428
AjuI GAANNNNNNNTTGG 2 cut(s) 611, 643
AloI GAACNNNNNNTCC 2 cut(s) 399, 431
AluBI AGCT 9 cut(s) 214, 259, 494, 499, 761, 883, 2257, 2393, 2543
AluI AGCT 9 cut(s) 214, 259, 494, 499, 761, 883, 2257, 2393, 2543
Alw21I GWGCWC 1 cut(s) 1574
Alw26I GTCTC 7 cut(s) 26, 241, 351, 1326, 1393, 1534, 1741
AlwI GGATC 7 cut(s) 758, 1145, 1980, 2174, 2229, 2360, 2477
AlwNI CAGNNNCTG 1 cut(s) 883
Ama87I CYCGRG 2 cut(s) 31, 767
Aor13HI TCCGGA 1 cut(s) 380
AoxI GGCC 5 cut(s) 776, 1584, 1618, 1648, 2344
ApeKI GCWGC 8 cut(s) 79, 425, 880, 926, 2190, 2254, 2390, 2440
ApoI RAATTY 7 cut(s) 86, 251, 514, 781, 1718, 2019, 2534
ArsI GACNNNNNNTTYG 2 cut(s) 236, 268
Asp718I GGTACC 1 cut(s) 1654
AspLEI GCGC 3 cut(s) 1317, 1412, 1738
AspS9I GGNCC 5 cut(s) 1648, 1872, 2056, 2374, 2432
AsuC2I CCSGG 4 cut(s) 54, 676, 768, 769
AsuHPI GGTGA 3 cut(s) 1780, 1931, 2100
AsuII TTCGAA 1 cut(s) 2495
AvaI CYCGRG 2 cut(s) 31, 767
AvaII GGWCC 4 cut(s) 1872, 2056, 2374, 2432
BaeI ACNNNNGTAYC 2 cut(s) 289, 322
BalI TGGCCA 2 cut(s) 1586, 1620
BanI GGYRCC 1 cut(s) 1654
BauI CACGAG 2 cut(s) 846, 1920
BbsI GAAGAC 1 cut(s) 2274
Bbv12I GWGCWC 1 cut(s) 1574
BbvI GCAGC 8 cut(s) 91, 412, 892, 913, 2202, 2266, 2402, 2452
BccI CCATC 3 cut(s) 1067, 1294, 2494
BceAI ACGGC 1 cut(s) 238
BciT130I CCWGG 9 cut(s) 530, 594, 669, 1023, 1652, 1817, 1943, 2051, 2430
BclI TGATCA 1 cut(s) 904
BcnI CCSGG 4 cut(s) 54, 676, 768, 769
BcoDI GTCTC 7 cut(s) 26, 241, 351, 1326, 1393, 1534, 1741
BcuI ACTAGT 2 cut(s) 61, 1579
BfaI CTAG 6 cut(s) 62, 233, 389, 1536, 1580, 1997
BfmI CTRYAG 4 cut(s) 682, 840, 2063, 2470
BfoI RGCGCY 1 cut(s) 1413
BfrI CTTAAG 1 cut(s) 1219
BisI GCNGC 9 cut(s) 80, 426, 881, 927, 1478, 2191, 2255, 2391, 2441
BlpI GCTNAGC 1 cut(s) 495
BlsI GCNGC 9 cut(s) 81, 427, 882, 928, 1479, 2192, 2256, 2392, 2442
Bme18I GGWCC 4 cut(s) 1872, 2056, 2374, 2432
BmeT110I CYCGRG 2 cut(s) 31, 767
BmgT120I GGNCC 5 cut(s) 1648, 1872, 2056, 2374, 2432
BmiI GGNNCC 3 cut(s) 673, 1656, 2057
BmsI GCATC 7 cut(s) 88, 886, 1147, 1169, 1990, 2321, 2429
BpiI GAAGAC 1 cut(s) 2274
BplI GAGNNNNNCTC 2 cut(s) 1396, 1428
BpmI CTGGAG 2 cut(s) 268, 2412
Bpu10I CCTNAGC 2 cut(s) 1568, 2436
Bpu1102I GCTNAGC 1 cut(s) 495
Bpu14I TTCGAA 1 cut(s) 2495
BpuEI CTTGAG 2 cut(s) 1547, 1933
BpuMI CCSGG 4 cut(s) 54, 676, 768, 769
Bsa29I ATCGAT 1 cut(s) 1163
BsaAI YACGTR 2 cut(s) 297, 1510
BsaWI WCCGGW 1 cut(s) 380
Bsc4I CCNNNNNNNGG 6 cut(s) 52, 674, 774, 1476, 2065, 2386
Bse1I ACTGG 3 cut(s) 46, 285, 1443
Bse3DI GCAATG 1 cut(s) 799
BseAI TCCGGA 1 cut(s) 380
BseBI CCWGG 9 cut(s) 530, 594, 669, 1023, 1652, 1817, 1943, 2051, 2430
BseCI ATCGAT 1 cut(s) 1163
BseGI GGATG 4 cut(s) 12, 182, 1078, 2155
BseLI CCNNNNNNNGG 6 cut(s) 52, 674, 774, 1476, 2065, 2386
BseMI GCAATG 1 cut(s) 799
BseMII CTCAG 4 cut(s) 509, 1539, 1559, 1995
BseNI ACTGG 3 cut(s) 46, 285, 1443
BseRI GAGGAG 3 cut(s) 152, 1344, 2415
BseXI GCAGC 8 cut(s) 91, 412, 892, 913, 2202, 2266, 2402, 2452
BseYI CCCAGC 1 cut(s) 428
BsgI GTGCAG 2 cut(s) 163, 912
Bsh1236I CGCG 1 cut(s) 1347
BshFI GGCC 5 cut(s) 778, 1586, 1620, 1650, 2346
BshNI GGYRCC 1 cut(s) 1654
BshVI ATCGAT 1 cut(s) 1163
BsiHKAI GWGCWC 1 cut(s) 1574
BsiHKCI CYCGRG 2 cut(s) 31, 767
BsiSI CCGG 4 cut(s) 53, 381, 675, 768
BsiWI CGTACG 1 cut(s) 297
BslFI GGGAC 3 cut(s) 692, 1133, 2069
BslI CCNNNNNNNGG 6 cut(s) 52, 674, 774, 1476, 2065, 2386
BsmAI GTCTC 7 cut(s) 26, 241, 351, 1326, 1393, 1534, 1741
BsmBI CGTCTC 1 cut(s) 1393
BsmFI GGGAC 3 cut(s) 692, 1133, 2069
BsmI GAATGC 1 cut(s) 2334
BsnI GGCC 5 cut(s) 778, 1586, 1620, 1650, 2346
BsoBI CYCGRG 2 cut(s) 31, 767
Bsp119I TTCGAA 1 cut(s) 2495
Bsp1286I GDGCHC 1 cut(s) 1574
Bsp13I TCCGGA 1 cut(s) 380
Bsp1720I GCTNAGC 1 cut(s) 495
Bsp19I CCATGG 1 cut(s) 2385
BspACI CCGC 5 cut(s) 711, 851, 1267, 1477, 2377
BspANI GGCC 5 cut(s) 778, 1586, 1620, 1650, 2346
BspCNI CTCAG 4 cut(s) 508, 1540, 1560, 1996
BspDI ATCGAT 1 cut(s) 1163
BspEI TCCGGA 1 cut(s) 380
BspFNI CGCG 1 cut(s) 1347
BspLI GGNNCC 3 cut(s) 673, 1656, 2057
BspMAI CTGCAG 2 cut(s) 2067, 2474
BspPI GGATC 7 cut(s) 758, 1145, 1980, 2174, 2229, 2360, 2477
BspT104I TTCGAA 1 cut(s) 2495
BspT107I GGYRCC 1 cut(s) 1654
BspTI CTTAAG 1 cut(s) 1219
BsrBI CCGCTC 1 cut(s) 851
BsrDI GCAATG 1 cut(s) 799
BsrI ACTGG 3 cut(s) 46, 285, 1443
BssSI CACGAG 2 cut(s) 846, 1920
BssT1I CCWWGG 2 cut(s) 1621, 2385
Bst2BI CACGAG 2 cut(s) 846, 1920
Bst2UI CCWGG 9 cut(s) 530, 594, 669, 1023, 1652, 1817, 1943, 2051, 2430
Bst4CI ACNGT 8 cut(s) 7, 337, 686, 836, 960, 1176, 1639, 2207
BstAFI CTTAAG 1 cut(s) 1219
BstAPI GCANNNNNTGC 1 cut(s) 2469
BstBAI YACGTR 2 cut(s) 297, 1510
BstBI TTCGAA 1 cut(s) 2495
BstC8I GCNNGC 6 cut(s) 43, 1212, 1216, 1471, 1475, 2445
BstDEI CTNAG 6 cut(s) 495, 1548, 1568, 2004, 2278, 2436
BstDSI CCRYGG 1 cut(s) 2385
BstF5I GGATG 4 cut(s) 12, 182, 1078, 2155
BstFNI CGCG 1 cut(s) 1347
BstH2I RGCGCY 1 cut(s) 1413
BstHHI GCGC 3 cut(s) 1317, 1412, 1738
BstMAI GTCTC 7 cut(s) 26, 241, 351, 1326, 1393, 1534, 1741
BstMWI GCNNNNNNNGC 4 cut(s) 42, 981, 1742, 2469
BstNI CCWGG 9 cut(s) 530, 594, 669, 1023, 1652, 1817, 1943, 2051, 2430
BstNSI RCATGY 1 cut(s) 2249
BstSFI CTRYAG 4 cut(s) 682, 840, 2063, 2470
BstUI CGCG 1 cut(s) 1347
BstV1I GCAGC 8 cut(s) 91, 412, 892, 913, 2202, 2266, 2402, 2452
BstV2I GAAGAC 1 cut(s) 2274
BstX2I RGATCY 3 cut(s) 1150, 1972, 2365
BstXI CCANNNNNNTGG 1 cut(s) 1582
BstYI RGATCY 3 cut(s) 1150, 1972, 2365
Bsu15I ATCGAT 1 cut(s) 1163
BsuRI GGCC 5 cut(s) 778, 1586, 1620, 1650, 2346
BsuTUI ATCGAT 1 cut(s) 1163
BtgI CCRYGG 1 cut(s) 2385
BtsCI GGATG 4 cut(s) 12, 182, 1078, 2155
BtsI GCAGTG 1 cut(s) 2127
BtsIMutI CAGTG 4 cut(s) 914, 965, 1635, 2127
Cac8I GCNNGC 6 cut(s) 43, 1212, 1216, 1471, 1475, 2445
CaiI CAGNNNCTG 1 cut(s) 883
CfoI GCGC 3 cut(s) 1317, 1412, 1738
Cfr13I GGNCC 5 cut(s) 1648, 1872, 2056, 2374, 2432
Cfr9I CCCGGG 1 cut(s) 767
ClaI ATCGAT 1 cut(s) 1163
Csp6I GTAC 8 cut(s) 298, 403, 808, 952, 1288, 1436, 1451, 1655
CviQI GTAC 8 cut(s) 298, 403, 808, 952, 1288, 1436, 1451, 1655
DdeI CTNAG 6 cut(s) 495, 1548, 1568, 2004, 2278, 2436
DrdI GACNNNNNNGTC 2 cut(s) 233, 365
DseDI GACNNNNNNGTC 2 cut(s) 233, 365
EaeI YGGCCR 3 cut(s) 776, 1584, 1618
EciI GGCGGA 1 cut(s) 1282
Eco130I CCWWGG 2 cut(s) 1621, 2385
Eco47I GGWCC 4 cut(s) 1872, 2056, 2374, 2432
Eco57I CTGAAG 2 cut(s) 253, 1683
Eco88I CYCGRG 2 cut(s) 31, 767
EcoO109I RGGNCCY 2 cut(s) 1872, 2432
EcoRI GAATTC 1 cut(s) 1718
EcoRII CCWGG 9 cut(s) 528, 592, 667, 1021, 1650, 1815, 1941, 2049, 2428
EcoT14I CCWWGG 2 cut(s) 1621, 2385
EcoT22I ATGCAT 2 cut(s) 1162, 2336
ErhI CCWWGG 2 cut(s) 1621, 2385
Esp3I CGTCTC 1 cut(s) 1393
FaqI GGGAC 3 cut(s) 692, 1133, 2069
FauNDI CATATG 2 cut(s) 977, 2095
FbaI TGATCA 1 cut(s) 904
Fnu4HI GCNGC 9 cut(s) 80, 426, 881, 927, 1478, 2191, 2255, 2391, 2441
FokI GGATG 3 cut(s) 169, 1085, 2162
Fsp4HI GCNGC 9 cut(s) 80, 426, 881, 927, 1478, 2191, 2255, 2391, 2441
FspAI RTGCGCAY 1 cut(s) 1316
FspBI CTAG 6 cut(s) 62, 233, 389, 1536, 1580, 1997
FspI TGCGCA 1 cut(s) 1316
GlaI GCGC 3 cut(s) 1316, 1411, 1737
GluI GCNGC 9 cut(s) 80, 426, 881, 927, 1478, 2191, 2255, 2391, 2441
GsaI CCCAGC 1 cut(s) 432
GsuI CTGGAG 2 cut(s) 268, 2412
HaeII RGCGCY 1 cut(s) 1413
HaeIII GGCC 5 cut(s) 778, 1586, 1620, 1650, 2346
HapII CCGG 4 cut(s) 53, 381, 675, 768
HhaI GCGC 3 cut(s) 1317, 1412, 1738
Hin6I GCGC 3 cut(s) 1315, 1410, 1736
HinP1I GCGC 3 cut(s) 1315, 1410, 1736
HincII GTYRAC 3 cut(s) 10, 370, 450
HindII GTYRAC 3 cut(s) 10, 370, 450
HpaII CCGG 4 cut(s) 53, 381, 675, 768
HphI GGTGA 3 cut(s) 1780, 1931, 2100
Hpy166II GTNNAC 7 cut(s) 10, 59, 67, 370, 450, 1019, 1351
Hpy8I GTNNAC 7 cut(s) 10, 59, 67, 370, 450, 1019, 1351
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 3 cut(s) 1256, 1863, 2491
HpyCH4III ACNGT 8 cut(s) 7, 337, 686, 836, 960, 1176, 1639, 2207
HpyCH4IV ACGT 4 cut(s) 296, 300, 1133, 1509
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 981, 1742, 2469
HpyF3I CTNAG 6 cut(s) 495, 1548, 1568, 2004, 2278, 2436
HpySE526I ACGT 4 cut(s) 296, 300, 1133, 1509
HspAI GCGC 3 cut(s) 1315, 1410, 1736
Kpn2I TCCGGA 1 cut(s) 380
KpnI GGTACC 1 cut(s) 1658
Ksp22I TGATCA 1 cut(s) 904
Lsp1109I GCAGC 8 cut(s) 91, 412, 892, 913, 2202, 2266, 2402, 2452
LweI GCATC 7 cut(s) 88, 886, 1147, 1169, 1990, 2321, 2429
MaeI CTAG 6 cut(s) 62, 233, 389, 1536, 1580, 1997
MaeII ACGT 4 cut(s) 296, 300, 1133, 1509
MaeIII GTNAC 3 cut(s) 944, 1129, 2106
MbiI CCGCTC 1 cut(s) 851
MfeI CAATTG 2 cut(s) 613, 1225
MflI RGATCY 3 cut(s) 1150, 1972, 2365
MhlI GDGCHC 1 cut(s) 1574
MlsI TGGCCA 2 cut(s) 1586, 1620
MluNI TGGCCA 2 cut(s) 1586, 1620
MlyI GAGTC 3 cut(s) 23, 1940, 2312
MmeI TCCRAC 6 cut(s) 609, 1050, 1239, 1860, 2068, 2127
Mox20I TGGCCA 2 cut(s) 1586, 1620
Mph1103I ATGCAT 2 cut(s) 1162, 2336
MroI TCCGGA 1 cut(s) 380
MscI TGGCCA 2 cut(s) 1586, 1620
MseI TTAA 7 cut(s) 396, 735, 996, 1182, 1220, 1608, 1713
MslI CAYNNNNRTG 2 cut(s) 1310, 1580
Msp20I TGGCCA 2 cut(s) 1586, 1620
MspA1I CMGCKG 3 cut(s) 883, 2257, 2393
MspCI CTTAAG 1 cut(s) 1219
MspI CCGG 4 cut(s) 53, 381, 675, 768
MunI CAATTG 2 cut(s) 613, 1225
Mva1269I GAATGC 1 cut(s) 2334
MvaI CCWGG 9 cut(s) 530, 594, 669, 1023, 1652, 1817, 1943, 2051, 2430
MvnI CGCG 1 cut(s) 1347
MwoI GCNNNNNNNGC 4 cut(s) 42, 981, 1742, 2469
NciI CCSGG 4 cut(s) 54, 676, 768, 769
NcoI CCATGG 1 cut(s) 2385
NdeI CATATG 2 cut(s) 977, 2095
NlaIV GGNNCC 3 cut(s) 673, 1656, 2057
NmeAIII GCCGAG 1 cut(s) 849
NmuCI GTSAC 3 cut(s) 944, 1129, 2106
NsbI TGCGCA 1 cut(s) 1316
NsiI ATGCAT 2 cut(s) 1162, 2336
NspI RCATGY 1 cut(s) 2249
NspV TTCGAA 1 cut(s) 2495
PaeR7I CTCGAG 1 cut(s) 31
PciI ACATGT 1 cut(s) 2245
PctI GAATGC 1 cut(s) 2334
PfeI GAWTC 7 cut(s) 264, 506, 1054, 1762, 1927, 2291, 2501
Pfl23II CGTACG 1 cut(s) 297
PfoI TCCNGGA 3 cut(s) 528, 592, 1815
PkrI GCNGC 9 cut(s) 81, 427, 882, 928, 1479, 2192, 2256, 2392, 2442
PleI GAGTC 3 cut(s) 23, 1940, 2311
PpsI GAGTC 3 cut(s) 23, 1940, 2311
Ppu21I YACGTR 2 cut(s) 297, 1510
PpuMI RGGWCCY 2 cut(s) 1872, 2432
PscI ACATGT 1 cut(s) 2245
PsiI TTATAA 1 cut(s) 1644
Psp5II RGGWCCY 2 cut(s) 1872, 2432
Psp6I CCWGG 9 cut(s) 528, 592, 667, 1021, 1650, 1815, 1941, 2049, 2428
PspFI CCCAGC 1 cut(s) 428
PspGI CCWGG 9 cut(s) 528, 592, 667, 1021, 1650, 1815, 1941, 2049, 2428
PspLI CGTACG 1 cut(s) 297
PspN4I GGNNCC 3 cut(s) 673, 1656, 2057
PspPI GGNCC 5 cut(s) 1648, 1872, 2056, 2374, 2432
PspPPI RGGWCCY 2 cut(s) 1872, 2432
PspXI VCTCGAGB 1 cut(s) 31
PstI CTGCAG 2 cut(s) 2067, 2474
PstNI CAGNNNCTG 1 cut(s) 883
PsuI RGATCY 3 cut(s) 1150, 1972, 2365
PvuII CAGCTG 3 cut(s) 883, 2257, 2393
RsaI GTAC 8 cut(s) 299, 404, 809, 953, 1289, 1437, 1452, 1656
RsaNI GTAC 8 cut(s) 298, 403, 808, 952, 1288, 1436, 1451, 1655
RseI CAYNNNNRTG 2 cut(s) 1310, 1580
SaqAI TTAA 7 cut(s) 396, 735, 996, 1182, 1220, 1608, 1713
SatI GCNGC 9 cut(s) 80, 426, 881, 927, 1478, 2191, 2255, 2391, 2441
Sau96I GGNCC 5 cut(s) 1648, 1872, 2056, 2374, 2432
SchI GAGTC 3 cut(s) 23, 1940, 2312
SduI GDGCHC 1 cut(s) 1574
SfaNI GCATC 7 cut(s) 88, 886, 1147, 1169, 1990, 2321, 2429
SfcI CTRYAG 4 cut(s) 682, 840, 2063, 2470
Sfr274I CTCGAG 1 cut(s) 31
SfuI TTCGAA 1 cut(s) 2495
SinI GGWCC 4 cut(s) 1872, 2056, 2374, 2432
SlaI CTCGAG 1 cut(s) 31
SmaI CCCGGG 1 cut(s) 769
SmiMI CAYNNNNRTG 2 cut(s) 1310, 1580
SmlI CTYRAG 4 cut(s) 31, 1219, 1526, 1948
SmoI CTYRAG 4 cut(s) 31, 1219, 1526, 1948
SpeI ACTAGT 2 cut(s) 61, 1579
SsiI CCGC 5 cut(s) 711, 851, 1267, 1477, 2377
SspI AATATT 4 cut(s) 436, 1559, 1990, 2046
SspMI CTAG 6 cut(s) 62, 233, 389, 1536, 1580, 1997
StyI CCWWGG 2 cut(s) 1621, 2385
TaaI ACNGT 8 cut(s) 7, 337, 686, 836, 960, 1176, 1639, 2207
TaiI ACGT 4 cut(s) 299, 303, 1136, 1512
TaqI TCGA 7 cut(s) 32, 1163, 1359, 1563, 1857, 2495, 2545
TauI GCSGC 1 cut(s) 1480
TfiI GAWTC 7 cut(s) 264, 506, 1054, 1762, 1927, 2291, 2501
Tru1I TTAA 7 cut(s) 396, 735, 996, 1182, 1220, 1608, 1713
Tru9I TTAA 7 cut(s) 396, 735, 996, 1182, 1220, 1608, 1713
TscAI CASTG 4 cut(s) 914, 965, 1642, 2127
TseFI GTSAC 3 cut(s) 944, 1129, 2106
TseI GCWGC 8 cut(s) 79, 425, 880, 926, 2190, 2254, 2390, 2440
Tsp45I GTSAC 3 cut(s) 944, 1129, 2106
TspDTI ATGAA 9 cut(s) 79, 614, 1044, 1221, 1536, 1743, 2024, 2246, 2392
TspGWI ACGGA 3 cut(s) 420, 969, 1417
TspMI CCCGGG 1 cut(s) 767
TspRI CASTG 4 cut(s) 914, 965, 1642, 2127
Vha464I CTTAAG 1 cut(s) 1219
VpaK11BI GGWCC 4 cut(s) 1872, 2056, 2374, 2432
XapI RAATTY 7 cut(s) 86, 251, 514, 781, 1718, 2019, 2534
XbaI TCTAGA 1 cut(s) 1996
XceI RCATGY 1 cut(s) 2249
XcmI CCANNNNNNNNNTGG 2 cut(s) 1628, 1658
XhoI CTCGAG 1 cut(s) 31
XmaI CCCGGG 1 cut(s) 767
XspI CTAG 6 cut(s) 62, 233, 389, 1536, 1580, 1997
Zsp2I ATGCAT 2 cut(s) 1162, 2336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.