RLG00000023750

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000112
Physical Location & Seq
Reverse (-)
27798511 .. 27801734
3224 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023750

Sequence Viewer

Length: 2193 bp
ATGCTGTTGAAATCTCTGCTTCTCTTCCTCCTCTTCCAAGTTTGCACTTCCAGGGACACCATAAGATCGAACGAAGAACTGAAGGATGATGGTGGTGGACTTCTGGTGTCCAAAGAAAGCAGCTTTGAGTTGGGTTTCTTCAGCCCTGGAAATTCTAGCAACCGCTATGTTGGGATTTGGTATTCTCAATCTAAAGTATCTGAAAAAACAGTGGTGTGGATCGCAAACAGAGACAACCCCATCAATGATACCTCTGGAGTCTTCACCATAAACAGGTATGGAGACCTAGTTCTCTATGCTTATAACATGAAGACCAGTCCTATATGGTCTGCAGATGTGTCTCTTTCAGTCAAAAACACCAGCACCTTATCTGCATATGTACAGCTTTTGGATACAGGAAACTTAGTTATGTTCCAGAATGATAGAGACGGAATCTTTGTATGGCAAAGTTTTGATTATCCTACAGATACTTTAATCCCGGGTATGAAAGTTGGGATGAATTGGAAAACTGGGCTAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACCTTTAGGCTTCATAATTGGAGCCACATTGTCACACCCCAGTATTTTATTTATAAAGGTTTGTCTAAGTATTGGCGAGGTGATACAGAGCCTTGGCCTAATTCGATCCGAAATGCAGAGGAAATGTCTTACTTACTTACTTATAACACCAATAAACTTACAATAATGAAGTTGAAGTTTTCTGGGCTACATCAGCACTTGGTATGGAATGATGGTGATCATCAATGGAAGGAGTTGTGGTCTTCACCGAAATACAGCTGTGACTGGTACGGATACTGTGGTGCCAACAGCAAATGTAGCTTTGACAGTGGTAATTTGTTCGAGTGTGAGTGTCTACCGGGGTATGAACCAAAATCTATAAATGGTTGGAATGGGAGAGATGGGTCAGATGGATGTGTGAGTAAACGAGTCGAAGTGTCAAAGTGTAGAAATGGAGAAGGGTTTGTGAAGGTGGAAAGGGTGAAAGATCCAGACGCATCGAGAGCAGCATTGTTGAGAATAGGTATGACTACCAAAGAGTGTGAGCAGGAATGCCTAAGAAATTGTTCTTGCACGGCATATATGACCAAAAAGATTGAAGGGGTTATTAATTGCTTGACATGGTATGATGACTTGATGGACATTTTGGTTTACACAGAGGCCGGACGAGATCTGTATGTTCGTGTGGATAAAATTGAGTTAGGTACTACATTTTTTGCCAACGAAAGTTTCAAAGATTTTGGTGAGTTACCAAATGGACAGAAGGTTGCTGTAAAAAGGCTGTCTAGCAGCTCAGGACAAGGGATTGGTGAATTCAAAAATGAAGTTGAACTGATAGCAGGACTTCAACACAGGAATCTTGTGAAGCTTTTAGGTTGTTGTATAAAGGGAGGAGAAAGGATGCTAGTCCTCGAATATATGCCGAACAAAAGCTTGGACTCATTTCTTTTTGATAACACAAGAAGGTCCTTCTTAGATTGGGGAAGGCGTTTTGAAATTATCAATGGGGTTGCTCGTGGGCTTTTGTATCTTCACCAAGACTCGAGATTAAGGATTATTCATAGGGATCTAAAAACCAGTAATATTCTTCTAGATGATGAGATGACCCCAAAAATTTCAGATTTCGGCATGGCAAGAATATTCCATGGGGACCAACTTCAGGATAACACAAAGAAAGTTGTTGGAACATATGGCTATATGTCACCAGAGTATGCAGTGTTTGGGAGATTCTCTACAAAATCGGATGTGTTTAGTTTTGGGATTATACTATTGGAGATTGTTAGTGGCAAGAGAAACAATGGTTCTTATGAGGAGAATTACTCCATGAACCTGATCGGACATGTTTGGCATGTGTGGAAAGAAGGAATAGCCTTAGATATTGTGGATTCATCGCTACAATCATATAACCATGATGAAGTCTTGAGATGCATTCAAGTTGGACTGTTGTGCGTGCAAGAAGATTCAAAGGACCGGCCAAGCATGTCATCCGTTGTGTTCATGTTGAGTGGTGAGGCATCTCTTCCGTCTCCTAAGCAGCCTGCATTTGTCTTCAGAAGAAACTCCAGCATTGCTGCTGATTCCTCGAGTCTAAAAGAATTGCATTCTATTAACGACTTGACAATAACTATTGTGGGAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

731

Amino Acids

82.8

Weight (kDa)

6.08

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 70 - 180 2.7e-34 D-mannose binding lectin
S_locus_glycop PF00954 229 - 309 3.5e-15 S-locus glycoprotein domain
PAN_2 PF08276 332 - 398 1.4e-17 PAN-like domain
PK_Tyr_Ser-Thr PF07714 435 - 681 8.2e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 435 - 680 9.3e-39 Protein kinase domain
DUF3403 PF11883 687 - 730 3.1e-09 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 303, 624, 714
AccB1I GGYRCC 1 cut(s) 851
AccI GTMKAC 1 cut(s) 904
AciI CCGC 1 cut(s) 163
AclWI GGATC 4 cut(s) 227, 670, 1031, 1623
AcoI YGGCCR 1 cut(s) 2021
AcsI RAATTY 3 cut(s) 151, 1361, 1662
AcuI CTGAAG 4 cut(s) 101, 124, 1691, 2083
AfaI GTAC 3 cut(s) 381, 839, 1255
AfiI CCNNNNNNNGG 3 cut(s) 273, 560, 1708
AflIII ACRYGT 1 cut(s) 1888
AjnI CCWGG 3 cut(s) 50, 145, 553
AjuI GAANNNNNNNTTGG 4 cut(s) 1262, 1294, 1466, 1498
AloI GAACNNNNNNTCC 2 cut(s) 273, 305
AluBI AGCT 8 cut(s) 123, 385, 828, 870, 1341, 1417, 1482, 2186
AluI AGCT 8 cut(s) 123, 385, 828, 870, 1341, 1417, 1482, 2186
Alw21I GWGCWC 1 cut(s) 2188
Alw26I GTCTC 5 cut(s) 225, 276, 345, 420, 2079
AlwI GGATC 4 cut(s) 227, 670, 1031, 1623
Ama87I CYCGRG 3 cut(s) 478, 1591, 2131
AoxI GGCC 3 cut(s) 665, 1209, 2021
ApeKI GCWGC 5 cut(s) 120, 1055, 1338, 2083, 2120
ApoI RAATTY 3 cut(s) 151, 1361, 1662
ArsI GACNNNNNNTTYG 2 cut(s) 419, 451
AseI ATTAAT 1 cut(s) 1158
Asp700I GAANNNNTTC 1 cut(s) 1114
AspS9I GGNCC 3 cut(s) 1515, 1699, 2017
AsuC2I CCSGG 3 cut(s) 479, 480, 909
AvaI CYCGRG 3 cut(s) 478, 1591, 2131
AvaII GGWCC 3 cut(s) 1515, 1699, 2017
BaeI ACNNNNGTAYC 2 cut(s) 835, 868
BanI GGYRCC 1 cut(s) 851
BanII GRGCYC 1 cut(s) 2188
BauI CACGAG 1 cut(s) 1563
BbsI GAAGAC 4 cut(s) 253, 317, 804, 2089
Bbv12I GWGCWC 1 cut(s) 2188
BbvI GCAGC 5 cut(s) 132, 1067, 1350, 2095, 2107
BccI CCATC 6 cut(s) 83, 248, 776, 944, 953, 1180
BceAI ACGGC 1 cut(s) 1140
BciT130I CCWGG 3 cut(s) 52, 147, 555
BciVI GTATCC 2 cut(s) 385, 836
BclI TGATCA 1 cut(s) 787
BcnI CCSGG 3 cut(s) 479, 480, 909
BcoDI GTCTC 5 cut(s) 225, 276, 345, 420, 2079
BfaI CTAG 6 cut(s) 156, 287, 515, 1335, 1454, 1640
BfmI CTRYAG 2 cut(s) 330, 462
BfuI GTATCC 2 cut(s) 385, 836
BglII AGATCT 1 cut(s) 1219
BisI GCNGC 5 cut(s) 121, 1056, 1339, 2084, 2121
BlsI GCNGC 5 cut(s) 122, 1057, 1340, 2085, 2122
Bme1390I CCNGG 6 cut(s) 52, 147, 479, 480, 555, 909
Bme18I GGWCC 3 cut(s) 1515, 1699, 2017
BmeT110I CYCGRG 3 cut(s) 478, 1591, 2131
BmgT120I GGNCC 3 cut(s) 1515, 1699, 2017
BmiI GGNNCC 3 cut(s) 593, 853, 1700
BmrFI CCNGG 6 cut(s) 52, 147, 479, 480, 555, 909
BmrI ACTGGG 3 cut(s) 519, 570, 604
BmsI GCATC 4 cut(s) 1055, 1440, 1964, 2072
BmuI ACTGGG 3 cut(s) 519, 570, 604
BpiI GAAGAC 4 cut(s) 253, 317, 804, 2089
BplI GAGNNNNNCTC 2 cut(s) 1853, 1885
BpmI CTGGAG 2 cut(s) 276, 2095
Bpu10I CCTNAGC 2 cut(s) 1342, 2079
BpuEI CTTGAG 1 cut(s) 1990
BpuMI CCSGG 3 cut(s) 479, 480, 909
BsaBI GATNNNNATC 1 cut(s) 786
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 7 cut(s) 51, 145, 478, 553, 662, 908, 1693
BsaXI ACNNNNNCTCC 4 cut(s) 273, 303, 794, 824
Bsc4I CCNNNNNNNGG 3 cut(s) 273, 560, 1708
Bse118I RCCGGY 1 cut(s) 2019
Bse1I ACTGG 6 cut(s) 315, 514, 565, 610, 839, 1626
Bse3DI GCAATG 1 cut(s) 2115
Bse8I GATNNNNATC 1 cut(s) 786
BseBI CCWGG 3 cut(s) 52, 147, 555
BseDI CCNNGG 7 cut(s) 51, 145, 478, 553, 662, 908, 1693
BseGI GGATG 6 cut(s) 91, 501, 968, 1455, 1798, 2033
BseJI GATNNNNATC 1 cut(s) 786
BseLI CCNNNNNNNGG 3 cut(s) 273, 560, 1708
BseMI GCAATG 1 cut(s) 2115
BseMII CTCAG 1 cut(s) 1356
BseNI ACTGG 6 cut(s) 315, 514, 565, 610, 839, 1626
BseRI GAGGAG 3 cut(s) 20, 1455, 1874
BseXI GCAGC 5 cut(s) 132, 1067, 1350, 2095, 2107
BshFI GGCC 3 cut(s) 667, 1211, 2023
BshNI GGYRCC 1 cut(s) 851
BsiHKAI GWGCWC 1 cut(s) 2188
BsiHKCI CYCGRG 3 cut(s) 478, 1591, 2131
BsiSI CCGG 4 cut(s) 479, 908, 1212, 2020
BslFI GGGAC 3 cut(s) 68, 578, 1712
BslI CCNNNNNNNGG 3 cut(s) 273, 560, 1708
BsmAI GTCTC 5 cut(s) 225, 276, 345, 420, 2079
BsmBI CGTCTC 2 cut(s) 420, 2079
BsmFI GGGAC 3 cut(s) 68, 578, 1712
BsmI GAATGC 3 cut(s) 1106, 1977, 2149
BsnI GGCC 3 cut(s) 667, 1211, 2023
Bso31I GGTCTC 1 cut(s) 276
BsoBI CYCGRG 3 cut(s) 478, 1591, 2131
Bsp1286I GDGCHC 1 cut(s) 2188
Bsp1407I TGTACA 1 cut(s) 379
Bsp143I GATC 8 cut(s) 65, 219, 675, 787, 1036, 1219, 1615, 1881
Bsp19I CCATGG 1 cut(s) 1693
BspACI CCGC 1 cut(s) 163
BspANI GGCC 3 cut(s) 667, 1211, 2023
BspCNI CTCAG 1 cut(s) 1355
BspLI GGNNCC 3 cut(s) 593, 853, 1700
BspMAI CTGCAG 1 cut(s) 334
BspPI GGATC 4 cut(s) 227, 670, 1031, 1623
BspT107I GGYRCC 1 cut(s) 851
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 1 cut(s) 2115
BsrFI RCCGGY 1 cut(s) 2019
BsrGI TGTACA 1 cut(s) 379
BsrI ACTGG 6 cut(s) 315, 514, 565, 610, 839, 1626
BssAI RCCGGY 1 cut(s) 2019
BssECI CCNNGG 7 cut(s) 51, 145, 478, 553, 662, 908, 1693
BssMI GATC 8 cut(s) 65, 219, 675, 787, 1036, 1219, 1615, 1881
BssSI CACGAG 1 cut(s) 1563
BssT1I CCWWGG 2 cut(s) 662, 1693
Bst2BI CACGAG 1 cut(s) 1563
Bst2UI CCWGG 3 cut(s) 52, 147, 555
Bst4CI ACNGT 4 cut(s) 211, 848, 878, 1992
Bst6I CTCTTC 3 cut(s) 29, 38, 2073
BstAUI TGTACA 1 cut(s) 379
BstC8I GCNNGC 2 cut(s) 2000, 2088
BstDEI CTNAG 7 cut(s) 403, 636, 1106, 1342, 1522, 1921, 2079
BstDSI CCRYGG 1 cut(s) 1693
BstF5I GGATG 6 cut(s) 91, 501, 968, 1455, 1798, 2033
BstKTI GATC 8 cut(s) 68, 222, 678, 790, 1039, 1222, 1618, 1884
BstMAI GTCTC 5 cut(s) 225, 276, 345, 420, 2079
BstMBI GATC 8 cut(s) 65, 219, 675, 787, 1036, 1219, 1615, 1881
BstMWI GCNNNNNNNGC 3 cut(s) 763, 867, 1052
BstNI CCWGG 3 cut(s) 52, 147, 555
BstNSI RCATGY 3 cut(s) 1892, 1901, 2032
BstSCI CCNGG 6 cut(s) 50, 145, 477, 478, 553, 907
BstSFI CTRYAG 2 cut(s) 330, 462
BstV1I GCAGC 5 cut(s) 132, 1067, 1350, 2095, 2107
BstV2I GAAGAC 4 cut(s) 253, 317, 804, 2089
BstX2I RGATCY 3 cut(s) 1036, 1219, 1615
BstYI RGATCY 3 cut(s) 1036, 1219, 1615
BsuI GTATCC 2 cut(s) 385, 836
BsuRI GGCC 3 cut(s) 667, 1211, 2023
BtgI CCRYGG 1 cut(s) 1693
BtgZI GCGATG 1 cut(s) 1923
BtsCI GGATG 6 cut(s) 91, 501, 968, 1455, 1798, 2033
BtsI GCAGTG 1 cut(s) 1770
BtsIMutI CAGTG 3 cut(s) 216, 883, 1770
Cac8I GCNNGC 2 cut(s) 2000, 2088
Cfr10I RCCGGY 1 cut(s) 2019
Cfr13I GGNCC 3 cut(s) 1515, 1699, 2017
Cfr9I CCCGGG 1 cut(s) 478
CseI GACGC 1 cut(s) 1052
Csp6I GTAC 3 cut(s) 380, 838, 1254
CviQI GTAC 3 cut(s) 380, 838, 1254
DdeI CTNAG 7 cut(s) 403, 636, 1106, 1342, 1522, 1921, 2079
DpnI GATC 8 cut(s) 67, 221, 677, 789, 1038, 1221, 1617, 1883
DpnII GATC 8 cut(s) 65, 219, 675, 787, 1036, 1219, 1615, 1881
EaeI YGGCCR 1 cut(s) 2021
Eam1104I CTCTTC 3 cut(s) 29, 38, 2073
EarI CTCTTC 3 cut(s) 29, 38, 2073
Ecl136II GAGCTC 1 cut(s) 2186
Eco130I CCWWGG 2 cut(s) 662, 1693
Eco24I GRGCYC 1 cut(s) 2188
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 3 cut(s) 1515, 1699, 2017
Eco53kI GAGCTC 1 cut(s) 2186
Eco57I CTGAAG 4 cut(s) 101, 124, 1691, 2083
Eco88I CYCGRG 3 cut(s) 478, 1591, 2131
EcoICRI GAGCTC 1 cut(s) 2186
EcoO109I RGGNCCY 1 cut(s) 1515
EcoRI GAATTC 1 cut(s) 1361
EcoRII CCWGG 3 cut(s) 50, 145, 553
EcoT14I CCWWGG 2 cut(s) 662, 1693
EcoT22I ATGCAT 1 cut(s) 1979
EcoT38I GRGCYC 1 cut(s) 2188
ErhI CCWWGG 2 cut(s) 662, 1693
Esp3I CGTCTC 2 cut(s) 420, 2079
FaqI GGGAC 3 cut(s) 68, 578, 1712
FauNDI CATATG 2 cut(s) 376, 1738
FbaI TGATCA 1 cut(s) 787
FblI GTMKAC 1 cut(s) 904
Fnu4HI GCNGC 5 cut(s) 121, 1056, 1339, 2084, 2121
FokI GGATG 6 cut(s) 98, 508, 975, 1462, 1805, 2020
FriOI GRGCYC 1 cut(s) 2188
Fsp4HI GCNGC 5 cut(s) 121, 1056, 1339, 2084, 2121
FspBI CTAG 6 cut(s) 156, 287, 515, 1335, 1454, 1640
GluI GCNGC 5 cut(s) 121, 1056, 1339, 2084, 2121
GsuI CTGGAG 2 cut(s) 276, 2095
HaeIII GGCC 3 cut(s) 667, 1211, 2023
HapII CCGG 4 cut(s) 479, 908, 1212, 2020
HgaI GACGC 1 cut(s) 1052
HindIII AAGCTT 2 cut(s) 1415, 1480
HpaII CCGG 4 cut(s) 479, 908, 1212, 2020
Hpy166II GTNNAC 4 cut(s) 98, 905, 974, 1201
Hpy188I TCNGA 7 cut(s) 202, 680, 958, 1669, 1792, 1886, 2102
Hpy188III TCNNGA 9 cut(s) 255, 415, 1040, 1050, 1344, 1593, 1640, 1709, 1969
Hpy8I GTNNAC 4 cut(s) 98, 905, 974, 1201
HpyCH4III ACNGT 4 cut(s) 211, 848, 878, 1992
HpyF10VI GCNNNNNNNGC 3 cut(s) 763, 867, 1052
HpyF3I CTNAG 7 cut(s) 403, 636, 1106, 1342, 1522, 1921, 2079
Ksp22I TGATCA 1 cut(s) 787
Kzo9I GATC 8 cut(s) 65, 219, 675, 787, 1036, 1219, 1615, 1881
LmnI GCTCC 2 cut(s) 591, 2183
Lsp1109I GCAGC 5 cut(s) 132, 1067, 1350, 2095, 2107
LweI GCATC 4 cut(s) 1055, 1440, 1964, 2072
MaeI CTAG 6 cut(s) 156, 287, 515, 1335, 1454, 1640
MaeIII GTNAC 5 cut(s) 540, 601, 830, 1296, 1749
MalI GATC 8 cut(s) 67, 221, 677, 789, 1038, 1221, 1617, 1883
MboI GATC 8 cut(s) 65, 219, 675, 787, 1036, 1219, 1615, 1881
MflI RGATCY 3 cut(s) 1036, 1219, 1615
MhlI GDGCHC 1 cut(s) 2188
MlyI GAGTC 5 cut(s) 267, 987, 1481, 1583, 2143
MmeI TCCRAC 3 cut(s) 917, 1711, 1966
Mph1103I ATGCAT 1 cut(s) 1979
MroXI GAANNNNTTC 1 cut(s) 1114
MseI TTAA 5 cut(s) 473, 527, 1158, 1598, 2157
MspA1I CMGCKG 1 cut(s) 828
MspI CCGG 4 cut(s) 479, 908, 1212, 2020
MspR9I CCNGG 6 cut(s) 52, 147, 479, 480, 555, 909
Mva1269I GAATGC 3 cut(s) 1106, 1977, 2149
MvaI CCWGG 3 cut(s) 52, 147, 555
MwoI GCNNNNNNNGC 3 cut(s) 763, 867, 1052
NciI CCSGG 3 cut(s) 479, 480, 909
NcoI CCATGG 1 cut(s) 1693
NdeI CATATG 2 cut(s) 376, 1738
NdeII GATC 8 cut(s) 65, 219, 675, 787, 1036, 1219, 1615, 1881
NlaIV GGNNCC 3 cut(s) 593, 853, 1700
NmuCI GTSAC 4 cut(s) 540, 601, 830, 1749
NsiI ATGCAT 1 cut(s) 1979
NspI RCATGY 3 cut(s) 1892, 1901, 2032
PaeR7I CTCGAG 2 cut(s) 1591, 2131
PciI ACATGT 1 cut(s) 1888
PctI GAATGC 3 cut(s) 1106, 1977, 2149
PdmI GAANNNNTTC 1 cut(s) 1114
PfeI GAWTC 6 cut(s) 432, 1405, 1776, 1934, 2009, 2126
PkrI GCNGC 5 cut(s) 122, 1057, 1340, 2085, 2122
PleI GAGTC 5 cut(s) 266, 986, 1481, 1583, 2142
PpsI GAGTC 5 cut(s) 266, 986, 1481, 1583, 2142
PpuMI RGGWCCY 1 cut(s) 1515
PscI ACATGT 1 cut(s) 1888
PshBI ATTAAT 1 cut(s) 1158
PsiI TTATAA 3 cut(s) 303, 624, 714
Psp124BI GAGCTC 1 cut(s) 2188
Psp5II RGGWCCY 1 cut(s) 1515
Psp6I CCWGG 3 cut(s) 50, 145, 553
PspGI CCWGG 3 cut(s) 50, 145, 553
PspN4I GGNNCC 3 cut(s) 593, 853, 1700
PspPI GGNCC 3 cut(s) 1515, 1699, 2017
PspPPI RGGWCCY 1 cut(s) 1515
PspXI VCTCGAGB 1 cut(s) 2131
PstI CTGCAG 1 cut(s) 334
PsuI RGATCY 3 cut(s) 1036, 1219, 1615
PvuII CAGCTG 1 cut(s) 828
RsaI GTAC 3 cut(s) 381, 839, 1255
RsaNI GTAC 3 cut(s) 380, 838, 1254
SacI GAGCTC 1 cut(s) 2188
SaqAI TTAA 5 cut(s) 473, 527, 1158, 1598, 2157
SatI GCNGC 5 cut(s) 121, 1056, 1339, 2084, 2121
Sau3AI GATC 8 cut(s) 65, 219, 675, 787, 1036, 1219, 1615, 1881
Sau96I GGNCC 3 cut(s) 1515, 1699, 2017
SchI GAGTC 5 cut(s) 267, 987, 1481, 1583, 2143
ScrFI CCNGG 6 cut(s) 52, 147, 479, 480, 555, 909
SduI GDGCHC 1 cut(s) 2188
SfaNI GCATC 4 cut(s) 1055, 1440, 1964, 2072
SfcI CTRYAG 2 cut(s) 330, 462
Sfr274I CTCGAG 2 cut(s) 1591, 2131
SinI GGWCC 3 cut(s) 1515, 1699, 2017
SlaI CTCGAG 2 cut(s) 1591, 2131
SmaI CCCGGG 1 cut(s) 480
SmlI CTYRAG 3 cut(s) 1591, 1969, 2131
SmoI CTYRAG 3 cut(s) 1591, 1969, 2131
SsiI CCGC 1 cut(s) 163
SspI AATATT 2 cut(s) 1633, 1689
SspMI CTAG 6 cut(s) 156, 287, 515, 1335, 1454, 1640
SstI GAGCTC 1 cut(s) 2188
StyD4I CCNGG 6 cut(s) 50, 145, 477, 478, 553, 907
StyI CCWWGG 2 cut(s) 662, 1693
TaaI ACNGT 4 cut(s) 211, 848, 878, 1992
TaqI TCGA 9 cut(s) 68, 674, 891, 981, 1049, 1461, 1592, 2132, 2188
TatI WGTACW 1 cut(s) 379
TfiI GAWTC 6 cut(s) 432, 1405, 1776, 1934, 2009, 2126
Tru1I TTAA 5 cut(s) 473, 527, 1158, 1598, 2157
Tru9I TTAA 5 cut(s) 473, 527, 1158, 1598, 2157
TscAI CASTG 3 cut(s) 216, 883, 1770
TseFI GTSAC 4 cut(s) 540, 601, 830, 1749
TseI GCWGC 5 cut(s) 120, 1055, 1338, 2083, 2120
Tsp45I GTSAC 4 cut(s) 540, 601, 830, 1749
TspGWI ACGGA 4 cut(s) 444, 855, 2026, 2061
TspMI CCCGGG 1 cut(s) 478
TspRI CASTG 3 cut(s) 216, 883, 1770
VpaK11BI GGWCC 3 cut(s) 1515, 1699, 2017
VspI ATTAAT 1 cut(s) 1158
XapI RAATTY 3 cut(s) 151, 1361, 1662
XbaI TCTAGA 1 cut(s) 1639
XceI RCATGY 3 cut(s) 1892, 1901, 2032
XhoI CTCGAG 2 cut(s) 1591, 2131
XmaI CCCGGG 1 cut(s) 478
XmiI GTMKAC 1 cut(s) 904
XmnI GAANNNNTTC 1 cut(s) 1114
XspI CTAG 6 cut(s) 156, 287, 515, 1335, 1454, 1640
Zsp2I ATGCAT 1 cut(s) 1979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.