RLG00000019148

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000112
Physical Location & Seq
Forward (+)
47388947 .. 47393298
4352 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019148

Sequence Viewer

Length: 2307 bp
ATGACAAAAAGAGAGCACATGAAGAAGGGACCTCCATGGGGAAGGACACCACAATCAGAGACCACGGTTCAGTGCTATGAACCTGCAACCATGAAATACTTGGGAGATTACCCTGCATTGACACCTGATGAGTTCTGCACTTCCAGGGACACCATAAAAGGGAATGAAGAACAACTGAAGGATGTTGATGGAGACTATTTAGTGTCCAAAGAAAGCGAGTTTGAATTGGGTTTCTTCAGTCCCGGAAATTCTAGCTACAGGTATGTTGGAATTTGGTATTCTCAGACTAGAGTATCCACTAAAACGGTGGTGTGGGTTGCAAACAGGAACAATCCCATCAATGATACCTCTGGTGTGCTCACAATAAACAGATATGGAGAACTAGTCCTTTATGCTTATAACATGGAGAGCACTCCTATTTGGTCTACTAATGTGTCGCGGTCGGTTCAAAATGTTAACACAAGCACTTTATCTGCACAGCTTTTAGATACAGGAAATTTAGTTGTGTTCCAGGCTGATAATAATGAAATCTATGTATGGCAAAGTTTTGATTATCCTACTGATACTTTAATTCCAGGTATGAAAGTTGGGGTGAATTGGAAAACTGGGCTAGAATGGGTTTTGACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACATTTAGGCTCTATCCATATCAGATCGCATCCCCCGAATTTTTTTTGTACAAGGGTTTGAGTAAGTATTACCGAACTGATCCAGGGCCAACGCCTGGTTTGGTCACTAGTCAAGAGGAAACTTATTATTCCATGAATGACACCAATGCAATTACAAGATTAACAGTGACTAATTCTGCGCTCGAGCGCCTTTATTGGGATGATGATGGCCTTCAATGGAAGGTAGACCTCTCTGCACCGAAGACCCGGTGTGACGGGTATGGAAAGTGTGGTGCCAACAGCAGATGTAGCCCTGATAACGTTAGTGTGTTTGAGTGTGAGTGTTTATCAGGGTACGTGCCTAAATCTATGAGTGATTGGAATCAGAAAAATGGGTCGGGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAAGTGTGGAGATGGAGACGGGTTTGTGAAGGTGGCAAATGTCAAATATCCAGATACATCGATAGCAGCATGGTTAAAATCAGGAATGAGTGCCAAAGAGTGTGAGCAGGAGTGCCTAAGAAATTGTTCTTGCAAAGCATATTTGAGCATTGAAAATGAAGGGATTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTTTTATGTACACAGAGTTTGGACGAGATCTCTACGTTCGTGTGAATGCAACCGTGTTAGATATCCTAAACATATCTAAGCTCCAAAAATCCCTTTTTGTTCTAGCAATATTAGGTTCTGGCCTGGGTCAGCTACCAAATGAACAAAAAGTTGCTGTGAAAAGATTGTCCAAAACTTCCGGACAAGGGATTGAGGAATTCAAAAATGAAGTTGCACTTATAGCAAGACTTCAACACAGAAATCTTGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATGTTAGTCCTAGAATACATGCCTAACAAAAGCTTGGACTTCTTTCTGTTTGATAACAGAAGACGGTCCTTCTTGGATTGGAAAAAGCGCTTTGAAATTATCAACGGGATTGCTCGTGGGATTCTGTATCTTCACCAAGACTCAAGATTGAGGATTATCCATAGAGATCTAAAAACTAGCAATGTTCTACTAGATGATGAGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATATTCGACCGGGACCAACTGCAAGATAAGACGAAACGAATTGTTGGAACATATGGCTACATGTCACCGGAGTACGCAGTATTTGGGAGATTTTCCACGAAATCTGATGTCTTTAGTTTTGGGATCATAATGTTGGAGATTGTAAGTGGGCAAAAAAACAATAGTTCTGATCTGGAGGATCCTTCCGTGAACTTGATAGGACGTGTTTGGGAGCTCTGGAGAGAAGGTAGAGCATTAGATATTGTTGATTCGACACTGAAGTCATGTCAGCCTAATGAGGTCATAAGATGCATACAAGTTGCGCTCTTGTGTGTACAAGAAGATTCAAAGGACCGACCTGCCATGTCAGCCATCGTTATCATGTTGAGTGGTGAAGCATCTCCTCCATTGCCTAAGCAGCCAGCATTTGTTTACAGAAGAGATTCCGGCACTGATGTTGATCCATTATTTCCAAACCGTTCTTCTTCTATAAACGACCTGACAATAACTGCAACGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

769

Amino Acids

86.82

Weight (kDa)

5.39

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 102 - 212 6.8e-36 D-mannose binding lectin
S_locus_glycop PF00954 260 - 338 3.6e-14 S-locus glycoprotein domain
PAN_2 PF08276 361 - 427 1.7e-17 PAN-like domain
PK_Tyr_Ser-Thr PF07714 473 - 720 7.4e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 473 - 718 2.2e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 399
AasI GACNNNNNNGTC 1 cut(s) 2059
Acc36I ACCTGC 2 cut(s) 91, 2146
AccB1I GGYRCC 1 cut(s) 938
AccB7I CCANNNNNTGG 1 cut(s) 761
AccI GTMKAC 2 cut(s) 425, 891
AccII CGCG 1 cut(s) 439
AccIII TCCGGA 1 cut(s) 1457
AciI CCGC 1 cut(s) 439
AclI AACGTT 1 cut(s) 966
AclWI GGATC 5 cut(s) 740, 1931, 1973, 1986, 2234
AcsI RAATTY 6 cut(s) 247, 270, 496, 704, 1475, 1776
AcuI CTGAAG 3 cut(s) 197, 220, 2078
AfaI GTAC 5 cut(s) 716, 1001, 1289, 1874, 2115
AfeI AGCGCT 1 cut(s) 1652
AfiI CCNNNNNNNGG 6 cut(s) 38, 159, 656, 761, 862, 1464
AflIII ACRYGT 2 cut(s) 1860, 2002
AgsI TTSAA 9 cut(s) 224, 449, 881, 1078, 1229, 1480, 1511, 1658, 2127
AhlI ACTAGT 2 cut(s) 382, 773
AjiI CACGTC 1 cut(s) 2003
AjnI CCWGG 7 cut(s) 143, 510, 574, 649, 748, 760, 1401
AjuI GAANNNNNNNTTGG 2 cut(s) 2245, 2277
AluBI AGCT 7 cut(s) 255, 481, 1360, 1411, 1596, 2014, 2300
AluI AGCT 7 cut(s) 255, 481, 1360, 1411, 1596, 2014, 2300
Alw21I GWGCWC 4 cut(s) 18, 360, 413, 2016
Alw26I GTCTC 3 cut(s) 53, 186, 1086
AlwI GGATC 5 cut(s) 740, 1931, 1973, 1986, 2234
Ama87I CYCGRG 1 cut(s) 848
Aor13HI TCCGGA 1 cut(s) 1457
Aor51HI AGCGCT 1 cut(s) 1652
AoxI GGCC 3 cut(s) 752, 874, 1399
ApeKI GCWGC 2 cut(s) 1142, 2197
ApoI RAATTY 6 cut(s) 247, 270, 496, 704, 1475, 1776
Asp700I GAANNNNTTC 2 cut(s) 1201, 2221
AspLEI GCGC 4 cut(s) 847, 855, 1653, 2104
AspS9I GGNCC 5 cut(s) 29, 752, 1629, 1813, 2131
AsuC2I CCSGG 3 cut(s) 243, 913, 1811
AsuHPI GGTGA 4 cut(s) 604, 1688, 1857, 2183
AvaI CYCGRG 1 cut(s) 848
AvaII GGWCC 4 cut(s) 29, 1629, 1813, 2131
BaeI ACNNNNGTAYC 2 cut(s) 276, 309
BamHI GGATCC 1 cut(s) 1978
BanI GGYRCC 1 cut(s) 938
BanII GRGCYC 1 cut(s) 2016
BauI CACGAG 1 cut(s) 1677
BbsI GAAGAC 2 cut(s) 914, 1630
Bbv12I GWGCWC 4 cut(s) 18, 360, 413, 2016
BbvI GCAGC 2 cut(s) 1154, 2209
BccI CCATC 6 cut(s) 182, 344, 866, 1082, 1267, 2159
BciT130I CCWGG 7 cut(s) 145, 512, 576, 651, 750, 762, 1403
BciVI GTATCC 1 cut(s) 304
BcnI CCSGG 3 cut(s) 243, 913, 1811
BcoDI GTCTC 3 cut(s) 53, 186, 1086
BcuI ACTAGT 2 cut(s) 382, 773
BfmI CTRYAG 1 cut(s) 256
BfoI RGCGCY 2 cut(s) 856, 1654
BfuAI ACCTGC 2 cut(s) 91, 2146
BfuI GTATCC 1 cut(s) 304
BglII AGATCT 2 cut(s) 1306, 1729
BisI GCNGC 2 cut(s) 1143, 2198
BlsI GCNGC 2 cut(s) 1144, 2199
Bme18I GGWCC 4 cut(s) 29, 1629, 1813, 2131
BmeT110I CYCGRG 1 cut(s) 848
BmgBI CACGTC 1 cut(s) 2003
BmgT120I GGNCC 5 cut(s) 29, 752, 1629, 1813, 2131
BmiI GGNNCC 4 cut(s) 30, 940, 1814, 1980
BmrI ACTGGG 2 cut(s) 615, 666
BmsI GCATC 3 cut(s) 704, 2078, 2186
BmuI ACTGGG 2 cut(s) 615, 666
BpiI GAAGAC 2 cut(s) 914, 1630
BpmI CTGGAG 2 cut(s) 1994, 2038
Bpu10I CCTNAGC 1 cut(s) 2193
BpuEI CTTGAG 1 cut(s) 1690
BpuMI CCSGG 3 cut(s) 243, 913, 1811
Bsa29I ATCGAT 1 cut(s) 1136
BsaAI YACGTR 1 cut(s) 1003
BsaBI GATNNNNATC 2 cut(s) 1026, 2238
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 6 cut(s) 35, 63, 144, 649, 749, 1402
BsaWI WCCGGW 2 cut(s) 1457, 1867
BsaXI ACNNNNNCTCC 4 cut(s) 369, 399, 2011, 2041
Bsc4I CCNNNNNNNGG 6 cut(s) 38, 159, 656, 761, 862, 1464
Bse1I ACTGG 2 cut(s) 610, 661
Bse3DI GCAATG 2 cut(s) 1750, 2186
Bse8I GATNNNNATC 2 cut(s) 1026, 2238
BseAI TCCGGA 1 cut(s) 1457
BseBI CCWGG 7 cut(s) 145, 512, 576, 651, 750, 762, 1403
BseCI ATCGAT 1 cut(s) 1136
BseDI CCNNGG 6 cut(s) 35, 63, 144, 649, 749, 1402
BseGI GGATG 5 cut(s) 187, 695, 871, 1055, 1569
BseJI GATNNNNATC 2 cut(s) 1026, 2238
BseLI CCNNNNNNNGG 6 cut(s) 38, 159, 656, 761, 862, 1464
BseMI GCAATG 2 cut(s) 1750, 2186
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 2 cut(s) 610, 661
BseRI GAGGAG 1 cut(s) 2172
BseXI GCAGC 2 cut(s) 1154, 2209
BsgI GTGCAG 3 cut(s) 121, 459, 885
Bsh1236I CGCG 1 cut(s) 439
Bsh1285I CGRYCG 2 cut(s) 443, 1810
BshFI GGCC 3 cut(s) 754, 876, 1401
BshNI GGYRCC 1 cut(s) 938
BshVI ATCGAT 1 cut(s) 1136
BsiEI CGRYCG 2 cut(s) 443, 1810
BsiHKAI GWGCWC 4 cut(s) 18, 360, 413, 2016
BsiHKCI CYCGRG 1 cut(s) 848
BsiSI CCGG 6 cut(s) 243, 913, 1458, 1810, 1868, 2226
BslFI GGGAC 5 cut(s) 42, 161, 225, 674, 1826
BslI CCNNNNNNNGG 6 cut(s) 38, 159, 656, 761, 862, 1464
BsmAI GTCTC 3 cut(s) 53, 186, 1086
BsmBI CGTCTC 1 cut(s) 1086
BsmFI GGGAC 5 cut(s) 42, 161, 225, 674, 1826
BsmI GAATGC 1 cut(s) 1330
BsnI GGCC 3 cut(s) 754, 876, 1401
Bso31I GGTCTC 1 cut(s) 53
BsoBI CYCGRG 1 cut(s) 848
Bsp1286I GDGCHC 4 cut(s) 18, 360, 413, 2016
Bsp13I TCCGGA 1 cut(s) 1457
Bsp1407I TGTACA 3 cut(s) 714, 1287, 2113
Bsp143I GATC 8 cut(s) 690, 745, 1306, 1729, 1923, 1969, 1978, 2239
Bsp19I CCATGG 1 cut(s) 35
BspACI CCGC 1 cut(s) 439
BspANI GGCC 3 cut(s) 754, 876, 1401
BspCNI CTCAG 1 cut(s) 295
BspDI ATCGAT 1 cut(s) 1136
BspEI TCCGGA 1 cut(s) 1457
BspFNI CGCG 1 cut(s) 439
BspLI GGNNCC 4 cut(s) 30, 940, 1814, 1980
BspMI ACCTGC 2 cut(s) 91, 2146
BspPI GGATC 5 cut(s) 740, 1931, 1973, 1986, 2234
BspT107I GGYRCC 1 cut(s) 938
BspTNI GGTCTC 1 cut(s) 53
BsrDI GCAATG 2 cut(s) 1750, 2186
BsrGI TGTACA 3 cut(s) 714, 1287, 2113
BsrI ACTGG 2 cut(s) 610, 661
BssECI CCNNGG 6 cut(s) 35, 63, 144, 649, 749, 1402
BssMI GATC 8 cut(s) 690, 745, 1306, 1729, 1923, 1969, 1978, 2239
BssSI CACGAG 1 cut(s) 1677
BssT1I CCWWGG 1 cut(s) 35
Bst2BI CACGAG 1 cut(s) 1677
Bst2UI CCWGG 7 cut(s) 145, 512, 576, 651, 750, 762, 1403
Bst4CI ACNGT 6 cut(s) 67, 307, 832, 1333, 1629, 2258
Bst6I CTCTTC 1 cut(s) 2212
BstAUI TGTACA 3 cut(s) 714, 1287, 2113
BstBAI YACGTR 1 cut(s) 1003
BstC8I GCNNGC 1 cut(s) 2202
BstDEI CTNAG 4 cut(s) 282, 1193, 1356, 2193
BstDSI CCRYGG 2 cut(s) 35, 63
BstF5I GGATG 5 cut(s) 187, 695, 871, 1055, 1569
BstFNI CGCG 1 cut(s) 439
BstH2I RGCGCY 2 cut(s) 856, 1654
BstHHI GCGC 4 cut(s) 847, 855, 1653, 2104
BstKTI GATC 8 cut(s) 693, 748, 1309, 1732, 1926, 1972, 1981, 2242
BstMAI GTCTC 3 cut(s) 53, 186, 1086
BstMBI GATC 8 cut(s) 690, 745, 1306, 1729, 1923, 1969, 1978, 2239
BstMCI CGRYCG 2 cut(s) 443, 1810
BstMWI GCNNNNNNNGC 5 cut(s) 954, 1499, 2147, 2197, 2297
BstNI CCWGG 7 cut(s) 145, 512, 576, 651, 750, 762, 1403
BstNSI RCATGY 2 cut(s) 1585, 1864
BstSFI CTRYAG 1 cut(s) 256
BstUI CGCG 1 cut(s) 439
BstV1I GCAGC 2 cut(s) 1154, 2209
BstV2I GAAGAC 2 cut(s) 914, 1630
BstX2I RGATCY 3 cut(s) 1306, 1729, 1978
BstYI RGATCY 3 cut(s) 1306, 1729, 1978
Bsu15I ATCGAT 1 cut(s) 1136
BsuI GTATCC 1 cut(s) 304
BsuRI GGCC 3 cut(s) 754, 876, 1401
BsuTUI ATCGAT 1 cut(s) 1136
BtgI CCRYGG 2 cut(s) 35, 63
BtrI CACGTC 1 cut(s) 2003
BtsCI GGATG 5 cut(s) 187, 695, 871, 1055, 1569
BtsIMutI CAGTG 4 cut(s) 77, 837, 2054, 2229
BveI ACCTGC 2 cut(s) 91, 2146
Cac8I GCNNGC 1 cut(s) 2202
CfoI GCGC 4 cut(s) 847, 855, 1653, 2104
Cfr13I GGNCC 5 cut(s) 29, 752, 1629, 1813, 2131
ClaI ATCGAT 1 cut(s) 1136
Csp6I GTAC 5 cut(s) 715, 1000, 1288, 1873, 2114
CviQI GTAC 5 cut(s) 715, 1000, 1288, 1873, 2114
DdeI CTNAG 4 cut(s) 282, 1193, 1356, 2193
DpnI GATC 8 cut(s) 692, 747, 1308, 1731, 1925, 1971, 1980, 2241
DpnII GATC 8 cut(s) 690, 745, 1306, 1729, 1923, 1969, 1978, 2239
DrdI GACNNNNNNGTC 1 cut(s) 2059
DseDI GACNNNNNNGTC 1 cut(s) 2059
Eam1104I CTCTTC 1 cut(s) 2212
EarI CTCTTC 1 cut(s) 2212
Ecl136II GAGCTC 1 cut(s) 2014
Eco130I CCWWGG 1 cut(s) 35
Eco24I GRGCYC 1 cut(s) 2016
Eco31I GGTCTC 1 cut(s) 53
Eco32I GATATC 1 cut(s) 1342
Eco47I GGWCC 4 cut(s) 29, 1629, 1813, 2131
Eco47III AGCGCT 1 cut(s) 1652
Eco53kI GAGCTC 1 cut(s) 2014
Eco57I CTGAAG 3 cut(s) 197, 220, 2078
Eco88I CYCGRG 1 cut(s) 848
EcoICRI GAGCTC 1 cut(s) 2014
EcoO109I RGGNCCY 1 cut(s) 29
EcoRI GAATTC 1 cut(s) 1475
EcoRII CCWGG 7 cut(s) 143, 510, 574, 649, 748, 760, 1401
EcoRV GATATC 1 cut(s) 1342
EcoT14I CCWWGG 1 cut(s) 35
EcoT22I ATGCAT 1 cut(s) 2093
EcoT38I GRGCYC 1 cut(s) 2016
ErhI CCWWGG 1 cut(s) 35
Esp3I CGTCTC 1 cut(s) 1086
FalI AAGNNNNNCTT 2 cut(s) 1479, 1511
FaqI GGGAC 5 cut(s) 42, 161, 225, 674, 1826
FauNDI CATATG 1 cut(s) 1852
FblI GTMKAC 2 cut(s) 425, 891
Fnu4HI GCNGC 2 cut(s) 1143, 2198
FokI GGATG 5 cut(s) 194, 682, 878, 1062, 1576
FriOI GRGCYC 1 cut(s) 2016
Fsp4HI GCNGC 2 cut(s) 1143, 2198
GlaI GCGC 4 cut(s) 846, 854, 1652, 2103
GluI GCNGC 2 cut(s) 1143, 2198
GsuI CTGGAG 2 cut(s) 1994, 2038
HaeII RGCGCY 2 cut(s) 856, 1654
HaeIII GGCC 3 cut(s) 754, 876, 1401
HapII CCGG 6 cut(s) 243, 913, 1458, 1810, 1868, 2226
HhaI GCGC 4 cut(s) 847, 855, 1653, 2104
Hin6I GCGC 4 cut(s) 845, 853, 1651, 2102
HinP1I GCGC 4 cut(s) 845, 853, 1651, 2102
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 1594
HinfI GANTC 6 cut(s) 1027, 1684, 1703, 2048, 2123, 2222
HpaI GTTAAC 1 cut(s) 457
HpaII CCGG 6 cut(s) 243, 913, 1458, 1810, 1868, 2226
HphI GGTGA 4 cut(s) 604, 1688, 1857, 2183
Hpy166II GTNNAC 7 cut(s) 426, 457, 892, 1290, 1990, 2114, 2212
Hpy188I TCNGA 7 cut(s) 58, 285, 690, 1032, 1783, 1906, 1969
Hpy188III TCNNGA 7 cut(s) 779, 1127, 1158, 1458, 1707, 1973, 2017
Hpy8I GTNNAC 7 cut(s) 426, 457, 892, 1290, 1990, 2114, 2212
HpyCH4III ACNGT 6 cut(s) 67, 307, 832, 1333, 1629, 2258
HpyCH4IV ACGT 4 cut(s) 966, 1002, 1314, 2002
HpyF10VI GCNNNNNNNGC 5 cut(s) 954, 1499, 2147, 2197, 2297
HpyF3I CTNAG 4 cut(s) 282, 1193, 1356, 2193
HpySE526I ACGT 4 cut(s) 966, 1002, 1314, 2002
HspAI GCGC 4 cut(s) 845, 853, 1651, 2102
Kpn2I TCCGGA 1 cut(s) 1457
KspAI GTTAAC 1 cut(s) 457
Kzo9I GATC 8 cut(s) 690, 745, 1306, 1729, 1923, 1969, 1978, 2239
LmnI GCTCC 2 cut(s) 1365, 2011
Lsp1109I GCAGC 2 cut(s) 1154, 2209
LweI GCATC 3 cut(s) 704, 2078, 2186
MaeII ACGT 4 cut(s) 966, 1002, 1314, 2002
MaeIII GTNAC 5 cut(s) 636, 769, 832, 917, 1863
MalI GATC 8 cut(s) 692, 747, 1308, 1731, 1925, 1971, 1980, 2241
MboI GATC 8 cut(s) 690, 745, 1306, 1729, 1923, 1969, 1978, 2239
MflI RGATCY 3 cut(s) 1306, 1729, 1978
MhlI GDGCHC 4 cut(s) 18, 360, 413, 2016
MlyI GAGTC 1 cut(s) 1697
MmeI TCCRAC 3 cut(s) 247, 1825, 1914
MnlI CCTC 9 cut(s) 42, 358, 775, 905, 1465, 1707, 1969, 2071, 2193
Mph1103I ATGCAT 1 cut(s) 2093
MroI TCCGGA 1 cut(s) 1457
MroXI GAANNNNTTC 2 cut(s) 1201, 2221
MseI TTAA 4 cut(s) 456, 569, 827, 1151
MslI CAYNNNNRTG 1 cut(s) 1283
MspI CCGG 6 cut(s) 243, 913, 1458, 1810, 1868, 2226
Mva1269I GAATGC 1 cut(s) 1330
MvaI CCWGG 7 cut(s) 145, 512, 576, 651, 750, 762, 1403
MvnI CGCG 1 cut(s) 439
MwoI GCNNNNNNNGC 5 cut(s) 954, 1499, 2147, 2197, 2297
NciI CCSGG 3 cut(s) 243, 913, 1811
NcoI CCATGG 1 cut(s) 35
NdeI CATATG 1 cut(s) 1852
NdeII GATC 8 cut(s) 690, 745, 1306, 1729, 1923, 1969, 1978, 2239
NlaIV GGNNCC 4 cut(s) 30, 940, 1814, 1980
NmuCI GTSAC 5 cut(s) 636, 769, 832, 917, 1863
NsiI ATGCAT 1 cut(s) 2093
NspI RCATGY 2 cut(s) 1585, 1864
PaeR7I CTCGAG 1 cut(s) 848
PciI ACATGT 1 cut(s) 1860
PcsI WCGNNNNNNNCGW 1 cut(s) 699
PctI GAATGC 1 cut(s) 1330
PdmI GAANNNNTTC 2 cut(s) 1201, 2221
PfeI GAWTC 5 cut(s) 1027, 1684, 2048, 2123, 2222
PflMI CCANNNNNTGG 1 cut(s) 761
PfoI TCCNGGA 1 cut(s) 241
PkrI GCNGC 2 cut(s) 1144, 2199
PleI GAGTC 1 cut(s) 1697
PpsI GAGTC 1 cut(s) 1697
Ppu21I YACGTR 1 cut(s) 1003
PpuMI RGGWCCY 1 cut(s) 29
PscI ACATGT 1 cut(s) 1860
PsiI TTATAA 1 cut(s) 399
Psp124BI GAGCTC 1 cut(s) 2016
Psp1406I AACGTT 1 cut(s) 966
Psp5II RGGWCCY 1 cut(s) 29
Psp6I CCWGG 7 cut(s) 143, 510, 574, 649, 748, 760, 1401
PspGI CCWGG 7 cut(s) 143, 510, 574, 649, 748, 760, 1401
PspN4I GGNNCC 4 cut(s) 30, 940, 1814, 1980
PspPI GGNCC 5 cut(s) 29, 752, 1629, 1813, 2131
PspPPI RGGWCCY 1 cut(s) 29
PspXI VCTCGAGB 1 cut(s) 848
PsuI RGATCY 3 cut(s) 1306, 1729, 1978
RsaI GTAC 5 cut(s) 716, 1001, 1289, 1874, 2115
RsaNI GTAC 5 cut(s) 715, 1000, 1288, 1873, 2114
RseI CAYNNNNRTG 1 cut(s) 1283
SacI GAGCTC 1 cut(s) 2016
SaqAI TTAA 4 cut(s) 456, 569, 827, 1151
SatI GCNGC 2 cut(s) 1143, 2198
Sau3AI GATC 8 cut(s) 690, 745, 1306, 1729, 1923, 1969, 1978, 2239
Sau96I GGNCC 5 cut(s) 29, 752, 1629, 1813, 2131
SchI GAGTC 1 cut(s) 1697
SduI GDGCHC 4 cut(s) 18, 360, 413, 2016
SfaNI GCATC 3 cut(s) 704, 2078, 2186
SfcI CTRYAG 1 cut(s) 256
Sfr274I CTCGAG 1 cut(s) 848
SinI GGWCC 4 cut(s) 29, 1629, 1813, 2131
SlaI CTCGAG 1 cut(s) 848
SmiMI CAYNNNNRTG 1 cut(s) 1283
SmlI CTYRAG 2 cut(s) 848, 1705
SmoI CTYRAG 2 cut(s) 848, 1705
SpeI ACTAGT 2 cut(s) 382, 773
SsiI CCGC 1 cut(s) 439
SspI AATATT 2 cut(s) 1389, 1803
SstI GAGCTC 1 cut(s) 2016
StyI CCWWGG 1 cut(s) 35
TaaI ACNGT 6 cut(s) 67, 307, 832, 1333, 1629, 2258
TaiI ACGT 4 cut(s) 969, 1005, 1317, 2005
TaqI TCGA 6 cut(s) 849, 1063, 1136, 1806, 2051, 2302
TaqII GACCGA 1 cut(s) 2148
TatI WGTACW 3 cut(s) 714, 1287, 2113
TfiI GAWTC 5 cut(s) 1027, 1684, 2048, 2123, 2222
Tru1I TTAA 4 cut(s) 456, 569, 827, 1151
Tru9I TTAA 4 cut(s) 456, 569, 827, 1151
TscAI CASTG 4 cut(s) 77, 837, 2061, 2236
TseFI GTSAC 5 cut(s) 636, 769, 832, 917, 1863
TseI GCWGC 2 cut(s) 1142, 2197
Tsp45I GTSAC 5 cut(s) 636, 769, 832, 917, 1863
TspGWI ACGGA 1 cut(s) 1975
TspRI CASTG 4 cut(s) 77, 837, 2061, 2236
Van91I CCANNNNNTGG 1 cut(s) 761
VpaK11BI GGWCC 4 cut(s) 29, 1629, 1813, 2131
XapI RAATTY 6 cut(s) 247, 270, 496, 704, 1475, 1776
XceI RCATGY 2 cut(s) 1585, 1864
XcmI CCANNNNNNNNNTGG 2 cut(s) 97, 304
XhoI CTCGAG 1 cut(s) 848
XmiI GTMKAC 2 cut(s) 425, 891
XmnI GAANNNNTTC 2 cut(s) 1201, 2221
Zsp2I ATGCAT 1 cut(s) 2093
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.