FvH4_6g00270

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000112
Physical Location & Seq
Forward (+)
207123 .. 210295
3173 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g00270.t1

Sequence Viewer

Length: 2451 bp
ATGTTTCCCAAAGTTCTGTTTCTCTTCCTGCTCTTTCAGTTCTGCACTTCCACGGACACCATAAAAGGGAACGAAGAGCAACTAAAGGATGTTGATGGAGACTACTTAGTGTCCAAGGAAAGCAAGTTTGAATTGGGGTTCTTCAGCCCCGGCAATTCTAGCTACCGGTATGTTGGAATTAGGTATTCTCAAGCTATAGTATCTGAAAGAACAGTGGTGTGGGTTGCAAACAGGAACAATCCCATCAATGATACCTCTGGTGTGCTCAAAATAAACAGGTATGGAGAACTGGCCCTCTATGCTTACAATTTGGAAAATGATCCTGTTTGGTTCACCAATGTGTCGCAGTCGGTCGAGAATGTAAACACCAGCACTTTATCAGCACAGCTTTTGGATACAGGAAATTTTGTCGTGTTCCAGGATGATAATAATGATATATTTATATGGCAGAGTTTTGATCATCCTACAGATACTCTACTTCCAGGTATGAAACTTGGGGTGAATTGGAAAACTGGGCTCGAATGGGTTTTAACATCTTGGAAGTCACATGATGACCCTGGAGCTGGGGAATATACACATAGGCTCTATCAAAATCAAAATCAGACTTATCTCCCCCAATTTTTTACATATAAAGGTTTGAGTAAGTATTGGCGAAGCGCTCCAGGGCCCTCTGCTACCTATGCTAGTTTTGTCAATAATCAAGAGGAAACTAGTTATGTAATGAATAAAACCAATGGAATTGGAAGACTAACTATGAATGGTTCTGAGGCGCAGCACCTTACATGGAGTGATGGAGACCATCAATGGAAGCAAGTCTATGCTGCACCCAAAGTACGGTGTGATTGGTATGCACATTGTGGTGCCAACAGCAAATGTTACCCTGAGATATTCGGTCTGTTTGAGTGTGATTGTCTACCCGGCTTTGAACCAAAATCTATCATTGAGTGGAACCAGAATGATGGGTCAGATGGATGTGTGAGTAAACGAAAGGAAGTTTCTAAGTGTGGGAATGGAGAAGGGTTTGTGCAGGTGACAAGAGTTAAATATCCAGACATATCAATAGCAGCTCGAGTGGCAGCACGCACGAATAACAAAGTGTGTAGGCAGGAGTGCTTGGCAAATTGTTCTTGCACAGCATATATGAGCATTGAAAATGAAAAAGGTGTTGTTGATTGTTGGACATGGTATGATGATTTGGTGGATATTCTTGTGTACACAGAGACGGGAAGAGATCTATATGTTCGTGTGGATCAAATGGAATTAGCTGAGAGTGCTGGAAAATCCAGAGGTTTCTTTAAAAGAAGGAGTATGCTTGCCATTCCTATAGTCTGTGCTCTGCTACCATTGGTACTGATCATTATGTGTGCCTGTTGGTGGTGTAAGAAACGAAGGAACACAACAGAAATTGTGGAGTCAGATGAACTTGAGGAAACTAAGAGGCACCCCGAATTGCAATTTTTCGATTTGAACACACTAATGGCAGCCACAGATAACTTCTCTCCTGTCAATGAACTCGGCCAAGGTGGTTTTGGCTCTGTTTATAAGGGTCAGTTACCAAACGAGCAGAAGGTTGCTGTGAAAAGGTTGTCTAAAACATCAGGACAAGGTACTGAAGAATTCAAAAACGAAGTTGCACTTATAGCAAGACTTCAACATCGCAATCTTGTAAAACTCTTAGGCTGTTGTATTAAGGGGGAAGAACGGATGTTAGTTCTAGAATACATGCCGAACAAAAGCTTGGACTCATTTCTCTTTGATCAGACAAGACGGTCCTTCTTGGATTGGGAAAAGCGCTTTGAAATCATCAACGGGATTGCTCGTGGGATCCTGTATCTTCACCAAGACTCAAGATTGAGGATTATCCATAGAGATCTAAAGACTAGCAATGTTCTTCTAGATGCTGAAATGAACCCGAAAATTTCTGATTTTGGCATGGCAAGAATATTTGACGGGGACCAAGTTCAGGATAAAACCAATCGAGTTGTTGGGACATATGGCTACATGTCACCAGAGTATGCGGTGTTCGGCAGATTTTCTACTAAATCGGATGTGTTTAGTTTTGGGATCATATTGTTAGAGGTTGTGAGTGGCAAAAGAAACAATTATTTTTCTCAAGAGGATCCTTCCATGAACTTGATTAGACATGTTTGGAACCTGTGGAGAGAAGACAGAGTCTTAGAAATTGTGGATTCAACTCTGGAGTTGTATAATTCTGATGAGGTCATGAGATGCATTCAAGTTGCGCTCTTGTGCGTGCAAGAAGAATCAGAGGACCGGCCAGCCATGTCTGCCATTGTTTTCATGTTGAGCGGCGAAACATCTCCTCCATCTCCTAACCAGCCTGCATATGTCATCAGAAGTTACTCCGTCACTGATGCTGATCCATCATTAGTACCAACACCATTTTATTCTATAAACGGCTTGACAATAACTGGTGAAGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

817

Amino Acids

92.91

Weight (kDa)

5.32

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 71 - 181 1e-35 D-mannose binding lectin
S_locus_glycop PF00954 229 - 312 5e-14 S-locus glycoprotein domain
PAN_2 PF08276 335 - 402 2.8e-15 PAN-like domain
Pkinase PF00069 502 - 764 3.3e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 503 - 767 1.1e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1542
AarI CACCTGC 1 cut(s) 1018
AasI GACNNNNNNGTC 1 cut(s) 1768
Acc36I ACCTGC 1 cut(s) 1018
AccB1I GGYRCC 2 cut(s) 860, 1440
AccBSI CCGCTC 1 cut(s) 2310
AccI GTMKAC 1 cut(s) 913
AciI CCGC 2 cut(s) 2018, 2310
AclWI GGATC 8 cut(s) 314, 1257, 1819, 1832, 2072, 2114, 2127, 2375
AcoI YGGCCR 2 cut(s) 1516, 2276
AcsI RAATTY 3 cut(s) 403, 1616, 1917
AcuI CTGAAG 2 cut(s) 127, 1632
AdeI CACNNNGTG 1 cut(s) 857
AfaI GTAC 5 cut(s) 834, 1214, 1350, 1609, 2394
AfeI AGCGCT 2 cut(s) 658, 1793
AfiI CCNNNNNNNGG 5 cut(s) 66, 563, 834, 1374, 1963
AflIII ACRYGT 2 cut(s) 2001, 2143
AgeI ACCGGT 1 cut(s) 165
AgsI TTSAA 9 cut(s) 131, 926, 1151, 1468, 1621, 1652, 1799, 2193, 2237
AhlI ACTAGT 1 cut(s) 710
AjnI CCWGG 4 cut(s) 417, 481, 556, 661
AjuI GAANNNNNNNTTGG 2 cut(s) 1721, 1753
AleI CACNNNNGTG 1 cut(s) 338
AluBI AGCT 8 cut(s) 162, 194, 388, 563, 1067, 1265, 1737, 2444
AluI AGCT 8 cut(s) 162, 194, 388, 563, 1067, 1265, 1737, 2444
Alw21I GWGCWC 2 cut(s) 267, 1336
Alw26I GTCTC 3 cut(s) 93, 789, 1214
AlwI GGATC 8 cut(s) 314, 1257, 1819, 1832, 2072, 2114, 2127, 2375
Ama87I CYCGRG 1 cut(s) 1068
Aor51HI AGCGCT 2 cut(s) 658, 1793
AoxI GGCC 4 cut(s) 291, 665, 1516, 2276
ApaI GGGCCC 1 cut(s) 669
ApeKI GCWGC 5 cut(s) 772, 821, 1064, 1076, 1481
ApoI RAATTY 3 cut(s) 403, 1616, 1917
AsiGI ACCGGT 1 cut(s) 165
AspLEI GCGC 4 cut(s) 659, 772, 1794, 2245
AspS9I GGNCC 6 cut(s) 292, 665, 666, 1770, 1954, 2272
AsuC2I CCSGG 2 cut(s) 150, 918
AsuHPI GGTGA 6 cut(s) 325, 511, 1042, 1829, 1998, 2447
AvaI CYCGRG 1 cut(s) 1068
AvaII GGWCC 3 cut(s) 1770, 1954, 2272
BaeGI GKGCMC 1 cut(s) 669
BamHI GGATCC 2 cut(s) 1824, 2119
BanI GGYRCC 2 cut(s) 860, 1440
BanII GRGCYC 2 cut(s) 519, 669
BauI CACGAG 1 cut(s) 1818
BbsI GAAGAC 2 cut(s) 751, 2172
Bbv12I GWGCWC 2 cut(s) 267, 1336
BbvI GCAGC 5 cut(s) 784, 808, 1076, 1088, 1493
BccI CCATC 8 cut(s) 89, 251, 785, 807, 953, 962, 2335, 2392
BceAI ACGGC 1 cut(s) 2434
BciT130I CCWGG 4 cut(s) 419, 483, 558, 663
BciVI GTATCC 1 cut(s) 388
BclI TGATCA 3 cut(s) 457, 1353, 1756
BcnI CCSGG 2 cut(s) 150, 918
BcoDI GTCTC 3 cut(s) 93, 789, 1214
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 6 cut(s) 159, 684, 711, 1715, 1881, 1895
BfmI CTRYAG 3 cut(s) 195, 465, 1323
BfoI RGCGCY 2 cut(s) 660, 1795
BfuAI ACCTGC 1 cut(s) 1018
BfuI GTATCC 1 cut(s) 388
BglII AGATCT 2 cut(s) 1231, 1870
BisI GCNGC 6 cut(s) 773, 822, 1065, 1077, 1482, 2311
BlsI GCNGC 6 cut(s) 774, 823, 1066, 1078, 1483, 2312
Bme1390I CCNGG 6 cut(s) 150, 419, 483, 558, 663, 918
Bme18I GGWCC 3 cut(s) 1770, 1954, 2272
BmeT110I CYCGRG 1 cut(s) 1068
BmgT120I GGNCC 6 cut(s) 292, 665, 666, 1770, 1954, 2272
BmiI GGNNCC 8 cut(s) 667, 862, 950, 1442, 1826, 1955, 2121, 2153
BmrFI CCNGG 6 cut(s) 150, 419, 483, 558, 663, 918
BmrI ACTGGG 1 cut(s) 522
BmsI GCATC 3 cut(s) 1888, 2219, 2365
BmuI ACTGGG 1 cut(s) 522
BpiI GAAGAC 2 cut(s) 751, 2172
BpmI CTGGAG 3 cut(s) 579, 645, 2219
BpuEI CTTGAG 4 cut(s) 174, 1445, 1831, 2097
BpuMI CCSGG 2 cut(s) 150, 918
BsaBI GATNNNNATC 1 cut(s) 2379
BsaI GGTCTC 1 cut(s) 789
BsaJI CCNNGG 6 cut(s) 51, 114, 148, 556, 662, 1519
BsaWI WCCGGW 1 cut(s) 165
BsaXI ACNNNNNCTCC 2 cut(s) 2308, 2338
Bsc4I CCNNNNNNNGG 5 cut(s) 66, 563, 834, 1374, 1963
Bse118I RCCGGY 2 cut(s) 165, 2274
Bse1I ACTGG 3 cut(s) 294, 517, 2437
Bse3DI GCAATG 1 cut(s) 1891
Bse8I GATNNNNATC 1 cut(s) 2379
BseBI CCWGG 4 cut(s) 419, 483, 558, 663
BseDI CCNNGG 6 cut(s) 51, 114, 148, 556, 662, 1519
BseGI GGATG 6 cut(s) 94, 427, 460, 977, 1710, 2053
BseJI GATNNNNATC 1 cut(s) 2379
BseLI CCNNNNNNNGG 5 cut(s) 66, 563, 834, 1374, 1963
BseMI GCAATG 1 cut(s) 1891
BseMII CTCAG 3 cut(s) 756, 873, 1257
BseNI ACTGG 3 cut(s) 294, 517, 2437
BseRI GAGGAG 1 cut(s) 2313
BseSI GKGCMC 1 cut(s) 669
BseXI GCAGC 5 cut(s) 784, 808, 1076, 1088, 1493
BseYI CCCAGC 1 cut(s) 563
BsgI GTGCAG 3 cut(s) 28, 807, 1046
Bsh1285I CGRYCG 1 cut(s) 354
BshFI GGCC 4 cut(s) 293, 667, 1518, 2278
BshNI GGYRCC 2 cut(s) 860, 1440
BshTI ACCGGT 1 cut(s) 165
BsiEI CGRYCG 1 cut(s) 354
BsiHKAI GWGCWC 2 cut(s) 267, 1336
BsiHKCI CYCGRG 1 cut(s) 1068
BsiSI CCGG 4 cut(s) 150, 166, 918, 2275
BslFI GGGAC 2 cut(s) 1967, 2002
BslI CCNNNNNNNGG 5 cut(s) 66, 563, 834, 1374, 1963
BsmAI GTCTC 3 cut(s) 93, 789, 1214
BsmBI CGTCTC 1 cut(s) 1214
BsmFI GGGAC 2 cut(s) 1967, 2002
BsmI GAATGC 1 cut(s) 2232
BsnI GGCC 4 cut(s) 293, 667, 1518, 2278
Bso31I GGTCTC 1 cut(s) 789
BsoBI CYCGRG 1 cut(s) 1068
Bsp120I GGGCCC 1 cut(s) 665
Bsp1286I GDGCHC 4 cut(s) 267, 519, 669, 1336
Bsp1407I TGTACA 1 cut(s) 1212
BspACI CCGC 2 cut(s) 2018, 2310
BspANI GGCC 4 cut(s) 293, 667, 1518, 2278
BspCNI CTCAG 3 cut(s) 757, 874, 1258
BspHI TCATGA 1 cut(s) 2223
BspLI GGNNCC 8 cut(s) 667, 862, 950, 1442, 1826, 1955, 2121, 2153
BspMI ACCTGC 1 cut(s) 1018
BspPI GGATC 8 cut(s) 314, 1257, 1819, 1832, 2072, 2114, 2127, 2375
BspQI GCTCTTC 1 cut(s) 69
BspT107I GGYRCC 2 cut(s) 860, 1440
BspTNI GGTCTC 1 cut(s) 789
BsrBI CCGCTC 1 cut(s) 2310
BsrDI GCAATG 1 cut(s) 1891
BsrFI RCCGGY 2 cut(s) 165, 2274
BsrGI TGTACA 1 cut(s) 1212
BsrI ACTGG 3 cut(s) 294, 517, 2437
BssAI RCCGGY 2 cut(s) 165, 2274
BssECI CCNNGG 6 cut(s) 51, 114, 148, 556, 662, 1519
BssSI CACGAG 1 cut(s) 1818
BssT1I CCWWGG 2 cut(s) 114, 1519
Bst2BI CACGAG 1 cut(s) 1818
Bst2UI CCWGG 4 cut(s) 419, 483, 558, 663
Bst4CI ACNGT 3 cut(s) 214, 837, 1770
Bst6I CTCTTC 3 cut(s) 29, 69, 1222
BstAUI TGTACA 1 cut(s) 1212
BstC8I GCNNGC 5 cut(s) 1081, 1314, 2255, 2280, 2343
BstDEI CTNAG 8 cut(s) 106, 765, 882, 999, 1266, 1434, 1675, 2176
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 6 cut(s) 94, 427, 460, 977, 1710, 2053
BstH2I RGCGCY 2 cut(s) 660, 1795
BstHHI GCGC 4 cut(s) 659, 772, 1794, 2245
BstMAI GTCTC 3 cut(s) 93, 789, 1214
BstMCI CGRYCG 1 cut(s) 354
BstMWI GCNNNNNNNGC 7 cut(s) 159, 299, 680, 1073, 1271, 1640, 2288
BstNI CCWGG 4 cut(s) 419, 483, 558, 663
BstNSI RCATGY 3 cut(s) 1726, 2005, 2147
BstSCI CCNGG 6 cut(s) 148, 417, 481, 556, 661, 916
BstSFI CTRYAG 3 cut(s) 195, 465, 1323
BstSLI GKGCMC 1 cut(s) 669
BstV1I GCAGC 5 cut(s) 784, 808, 1076, 1088, 1493
BstV2I GAAGAC 2 cut(s) 751, 2172
BstX2I RGATCY 4 cut(s) 1231, 1824, 1870, 2119
BstXI CCANNNNNNTGG 1 cut(s) 959
BstYI RGATCY 4 cut(s) 1231, 1824, 1870, 2119
BsuI GTATCC 1 cut(s) 388
BsuRI GGCC 4 cut(s) 293, 667, 1518, 2278
BtgI CCRYGG 1 cut(s) 51
BtgZI GCGATG 1 cut(s) 1640
BtsCI GGATG 6 cut(s) 94, 427, 460, 977, 1710, 2053
BtsIMutI CAGTG 2 cut(s) 219, 2370
BveI ACCTGC 1 cut(s) 1018
Cac8I GCNNGC 5 cut(s) 1081, 1314, 2255, 2280, 2343
CciI TCATGA 1 cut(s) 2223
CfoI GCGC 4 cut(s) 659, 772, 1794, 2245
Cfr10I RCCGGY 2 cut(s) 165, 2274
Cfr13I GGNCC 6 cut(s) 292, 665, 666, 1770, 1954, 2272
Csp6I GTAC 5 cut(s) 833, 1213, 1349, 1608, 2393
CspAI ACCGGT 1 cut(s) 165
CviQI GTAC 5 cut(s) 833, 1213, 1349, 1608, 2393
DdeI CTNAG 8 cut(s) 106, 765, 882, 999, 1266, 1434, 1675, 2176
DraI TTTAAA 1 cut(s) 1297
DraIII CACNNNGTG 1 cut(s) 857
DrdI GACNNNNNNGTC 1 cut(s) 1768
DseDI GACNNNNNNGTC 1 cut(s) 1768
EaeI YGGCCR 2 cut(s) 1516, 2276
Eam1104I CTCTTC 3 cut(s) 29, 69, 1222
EarI CTCTTC 3 cut(s) 29, 69, 1222
Eco130I CCWWGG 2 cut(s) 114, 1519
Eco24I GRGCYC 2 cut(s) 519, 669
Eco31I GGTCTC 1 cut(s) 789
Eco47I GGWCC 3 cut(s) 1770, 1954, 2272
Eco47III AGCGCT 2 cut(s) 658, 1793
Eco57I CTGAAG 2 cut(s) 127, 1632
Eco88I CYCGRG 1 cut(s) 1068
EcoO109I RGGNCCY 2 cut(s) 665, 666
EcoRI GAATTC 1 cut(s) 1616
EcoRII CCWGG 4 cut(s) 417, 481, 556, 661
EcoT14I CCWWGG 2 cut(s) 114, 1519
EcoT22I ATGCAT 1 cut(s) 2234
EcoT38I GRGCYC 2 cut(s) 519, 669
ErhI CCWWGG 2 cut(s) 114, 1519
Esp3I CGTCTC 1 cut(s) 1214
FalI AAGNNNNNCTT 2 cut(s) 1620, 1652
FaqI GGGAC 2 cut(s) 1967, 2002
FauNDI CATATG 2 cut(s) 1993, 2347
FbaI TGATCA 3 cut(s) 457, 1353, 1756
FblI GTMKAC 1 cut(s) 913
Fnu4HI GCNGC 6 cut(s) 773, 822, 1065, 1077, 1482, 2311
FokI GGATG 6 cut(s) 101, 434, 447, 984, 1717, 2060
FriOI GRGCYC 2 cut(s) 519, 669
Fsp4HI GCNGC 6 cut(s) 773, 822, 1065, 1077, 1482, 2311
FspBI CTAG 6 cut(s) 159, 684, 711, 1715, 1881, 1895
GlaI GCGC 4 cut(s) 658, 771, 1793, 2244
GluI GCNGC 6 cut(s) 773, 822, 1065, 1077, 1482, 2311
GsaI CCCAGC 1 cut(s) 567
GsuI CTGGAG 3 cut(s) 579, 645, 2219
HaeII RGCGCY 2 cut(s) 660, 1795
HaeIII GGCC 4 cut(s) 293, 667, 1518, 2278
HapII CCGG 4 cut(s) 150, 166, 918, 2275
HhaI GCGC 4 cut(s) 659, 772, 1794, 2245
Hin6I GCGC 4 cut(s) 657, 770, 1792, 2243
HinP1I GCGC 4 cut(s) 657, 770, 1792, 2243
HindIII AAGCTT 1 cut(s) 1735
HinfI GANTC 6 cut(s) 1412, 1742, 1844, 2172, 2189, 2264
HpaII CCGG 4 cut(s) 150, 166, 918, 2275
HphI GGTGA 6 cut(s) 325, 511, 1042, 1829, 1998, 2447
Hpy166II GTNNAC 6 cut(s) 333, 364, 914, 983, 1213, 1215
Hpy8I GTNNAC 6 cut(s) 333, 364, 914, 983, 1213, 1215
HpyAV CCTTC 6 cut(s) 1010, 1296, 1383, 1561, 1783, 2133
HpyCH4III ACNGT 3 cut(s) 214, 837, 1770
HpyF10VI GCNNNNNNNGC 7 cut(s) 159, 299, 680, 1073, 1271, 1640, 2288
HpyF3I CTNAG 8 cut(s) 106, 765, 882, 999, 1266, 1434, 1675, 2176
HspAI GCGC 4 cut(s) 657, 770, 1792, 2243
Ksp22I TGATCA 3 cut(s) 457, 1353, 1756
LguI GCTCTTC 1 cut(s) 69
LmnI GCTCC 2 cut(s) 560, 664
Lsp1109I GCAGC 5 cut(s) 784, 808, 1076, 1088, 1493
LweI GCATC 3 cut(s) 1888, 2219, 2365
MaeI CTAG 6 cut(s) 159, 684, 711, 1715, 1881, 1895
MaeIII GTNAC 7 cut(s) 543, 875, 1030, 1551, 2004, 2360, 2368
MbiI CCGCTC 1 cut(s) 2310
MflI RGATCY 4 cut(s) 1231, 1824, 1870, 2119
MhlI GDGCHC 4 cut(s) 267, 519, 669, 1336
MlyI GAGTC 4 cut(s) 1421, 1736, 1838, 2181
MmeI TCCRAC 2 cut(s) 154, 1157
Mph1103I ATGCAT 1 cut(s) 2234
MseI TTAA 4 cut(s) 530, 1041, 1296, 1689
MslI CAYNNNNRTG 3 cut(s) 338, 858, 1475
MspI CCGG 4 cut(s) 150, 166, 918, 2275
MspR9I CCNGG 6 cut(s) 150, 419, 483, 558, 663, 918
Mva1269I GAATGC 1 cut(s) 2232
MvaI CCWGG 4 cut(s) 419, 483, 558, 663
MwoI GCNNNNNNNGC 7 cut(s) 159, 299, 680, 1073, 1271, 1640, 2288
NciI CCSGG 2 cut(s) 150, 918
NdeI CATATG 2 cut(s) 1993, 2347
NlaIV GGNNCC 8 cut(s) 667, 862, 950, 1442, 1826, 1955, 2121, 2153
NmeAIII GCCGAG 1 cut(s) 1494
NmuCI GTSAC 4 cut(s) 543, 1030, 2004, 2368
NsiI ATGCAT 1 cut(s) 2234
NspI RCATGY 3 cut(s) 1726, 2005, 2147
OliI CACNNNNGTG 1 cut(s) 338
PaeR7I CTCGAG 1 cut(s) 1068
PagI TCATGA 1 cut(s) 2223
PaqCI CACCTGC 1 cut(s) 1018
PciI ACATGT 2 cut(s) 2001, 2143
PciSI GCTCTTC 1 cut(s) 69
PctI GAATGC 1 cut(s) 2232
PfeI GAWTC 2 cut(s) 2189, 2264
PflFI GACNNNGTC 1 cut(s) 2171
PfoI TCCNGGA 1 cut(s) 417
PinAI ACCGGT 1 cut(s) 165
PkrI GCNGC 6 cut(s) 774, 823, 1066, 1078, 1483, 2312
PleI GAGTC 4 cut(s) 1420, 1736, 1838, 2180
PpsI GAGTC 4 cut(s) 1420, 1736, 1838, 2180
PscI ACATGT 2 cut(s) 2001, 2143
PsiI TTATAA 1 cut(s) 1542
Psp6I CCWGG 4 cut(s) 417, 481, 556, 661
PspFI CCCAGC 1 cut(s) 563
PspGI CCWGG 4 cut(s) 417, 481, 556, 661
PspN4I GGNNCC 8 cut(s) 667, 862, 950, 1442, 1826, 1955, 2121, 2153
PspOMI GGGCCC 1 cut(s) 665
PspPI GGNCC 6 cut(s) 292, 665, 666, 1770, 1954, 2272
PspXI VCTCGAGB 1 cut(s) 1068
PsrI GAACNNNNNNTAC 2 cut(s) 2006, 2038
PsuI RGATCY 4 cut(s) 1231, 1824, 1870, 2119
PsyI GACNNNGTC 1 cut(s) 2171
RsaI GTAC 5 cut(s) 834, 1214, 1350, 1609, 2394
RsaNI GTAC 5 cut(s) 833, 1213, 1349, 1608, 2393
RseI CAYNNNNRTG 3 cut(s) 338, 858, 1475
SapI GCTCTTC 1 cut(s) 69
SaqAI TTAA 4 cut(s) 530, 1041, 1296, 1689
SatI GCNGC 6 cut(s) 773, 822, 1065, 1077, 1482, 2311
Sau96I GGNCC 6 cut(s) 292, 665, 666, 1770, 1954, 2272
SchI GAGTC 4 cut(s) 1421, 1736, 1838, 2181
ScrFI CCNGG 6 cut(s) 150, 419, 483, 558, 663, 918
SduI GDGCHC 4 cut(s) 267, 519, 669, 1336
SfaNI GCATC 3 cut(s) 1888, 2219, 2365
SfcI CTRYAG 3 cut(s) 195, 465, 1323
Sfr274I CTCGAG 1 cut(s) 1068
SinI GGWCC 3 cut(s) 1770, 1954, 2272
SlaI CTCGAG 1 cut(s) 1068
SmiMI CAYNNNNRTG 3 cut(s) 338, 858, 1475
SmlI CTYRAG 5 cut(s) 189, 1068, 1424, 1846, 2112
SmoI CTYRAG 5 cut(s) 189, 1068, 1424, 1846, 2112
SpeI ACTAGT 1 cut(s) 710
SsiI CCGC 2 cut(s) 2018, 2310
SspI AATATT 1 cut(s) 1944
SspMI CTAG 6 cut(s) 159, 684, 711, 1715, 1881, 1895
StyD4I CCNGG 6 cut(s) 148, 417, 481, 556, 661, 916
StyI CCWWGG 2 cut(s) 114, 1519
TaaI ACNGT 3 cut(s) 214, 837, 1770
TaqI TCGA 6 cut(s) 354, 519, 1069, 1461, 1978, 2446
TaqII GACCGA 2 cut(s) 340, 881
TatI WGTACW 1 cut(s) 1212
TauI GCSGC 1 cut(s) 2313
TfiI GAWTC 2 cut(s) 2189, 2264
Tru1I TTAA 4 cut(s) 530, 1041, 1296, 1689
Tru9I TTAA 4 cut(s) 530, 1041, 1296, 1689
TscAI CASTG 2 cut(s) 219, 2377
TseFI GTSAC 4 cut(s) 543, 1030, 2004, 2368
TseI GCWGC 5 cut(s) 772, 821, 1064, 1076, 1481
Tsp45I GTSAC 4 cut(s) 543, 1030, 2004, 2368
TspDTI ATGAA 9 cut(s) 503, 737, 770, 1170, 1434, 1524, 1922, 2144, 2290
TspGWI ACGGA 3 cut(s) 68, 1717, 2356
TspRI CASTG 2 cut(s) 219, 2377
Tth111I GACNNNGTC 1 cut(s) 2171
VpaK11BI GGWCC 3 cut(s) 1770, 1954, 2272
XapI RAATTY 3 cut(s) 403, 1616, 1917
XbaI TCTAGA 2 cut(s) 1714, 1894
XceI RCATGY 3 cut(s) 1726, 2005, 2147
XcmI CCANNNNNNNNNTGG 1 cut(s) 1526
XhoI CTCGAG 1 cut(s) 1068
XmiI GTMKAC 1 cut(s) 913
XspI CTAG 6 cut(s) 159, 684, 711, 1715, 1881, 1895
Zsp2I ATGCAT 1 cut(s) 2234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.