Rroxscaffold_1G00070540

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000112
Physical Location & Seq
Reverse (-)
91450683 .. 91454073
3391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00070540.1

Sequence Viewer

Length: 2433 bp
ATGTTTTTGAAAGCACTGCTTCTCTTCCTCCTCTTTCAGTTCTGCACTTCCAGGGACACCATAAAAGGGAATGAAGAACAATTGAAGGATGTTGTTGATGGAGACTATTTAGTCTCCAAAGAAAGCAAGTTTGAATTGGGTTTCTTCAGCCCCGGAAATTCTAGCGACTGGTATGTTGGAATCTGGTATTCTCAGACTAGAGTATCTAATAAAACAGTGGTGTGGGTTGCAAACAGAAACAATCCCATCAATGATACCTCTGGTGTGCTCACAATAAACAGGTATGGAGAACTAGTCCTTTATGCGTATAACATGAAGAGCACTCCTATTTGGTCTACTAACATGTCGCGGTCGGTTCAAAATGTTAACAGAAGCACTTTATCTGCACAGCTTTTAGATACAGGAAATTTAGTTGTGTTCCAGGATGATAAAAATGGAATCTCTATATGGCAAAGTTTTGATTATCCGACAGATACTCTAATTGTAGGTATGAAAGTTGGAGTGAATTGGAAAACTGGGCTAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCCGGAACTGGGGACTATACCTTTAGGCTTCATTCAGATCAGACTGCCTCCCCCGAATATTTTATGTATAAAGGTTTGAGCAAATATTGGCGAAGTGATCCAGGGCCAGGGCCTAGTTTGGTCACTAATCAAGAGGAAACTTATTATATCCTAAATTACACCGATGCGATTACAAGAATGACGTTGACTGATTCTGAGTTAGATCTTCTTACATGGGAAGATGGTAGCCTTCAATGGAAGAAAGAATTTTCTGCACCGAAGTCCCGGTGTGACTGGTATGGACAGTGTGGTGCCAACAGCAAATGTTTCCCTGACAATGTTAATGTGTTTGAGTGCGACTGTTTACCAGGGTATGAGCCTAGTTCTATAAATGATTGGAATCAGAAAAATGGGTCGGGTGGATGTGTGAGTAAGCGAGTTGGTTTATCAAAGTGTGGGAATGGAGATGGGTTTGTTAATCTGGCAAGAGTTAAATATCCAGACGCATCGATAGCATCACGGTCGAGAACAGGTATGAGTGCCAAAGAGTGTGAGCAGGACTGCTTAAGAAATTGTTCTTGCACCGCATATTTGACCATTGAAAATGATGGACTTGTGGATTGCTTGACATGGTACGATAATTTGATGGACATTTTAGAGTTCACAAAGTTTGGAAGAGATCTATATGTTCGTGTGGATCACATCGAATTAGCTGAGAATGCTAGAAAATCAAAAGGTTTCTTGGAAAGAAGGGGTATGCTGGCTATTCCAATACTCTGTGCTCTGCTAGCATTAGTACTAATCATTATGTGCGCCTGCTCGTGGCACAAGAAAAAGAGGAATACAACGGAATTTGTGGAGGCGGATGAACTGGAGGAAACCAAGAGACACCCCGAATTGCAATTTTTTGATTTGAACACACTAATGGCAGCCACAAATAACTTCTCTCATGTCAATGAACTTGGCCAAGGTGGTTTTGGGTCTGTTTATAAGGGTCAGTTACCAAATGAGCAAAAGGTAGCTGTAAAAAGGTTGTCTAAAACATCAGGACAAGGGACTGAAGAATTTAAAAATGAAGTTGCACTTATAGCAGGACTTCAACACCGCAATCTTGTAAAACTCTTAGGCTGTTGTATAAAGGGGGAAGAACGGATGTTAGTCCTGGAATACATGCCGAACAAAAGCTTGGACTCATTTCTCTTTGATCACAGAAGGCGGTCCTTCTTGGATTGGGAAAAGCGTTTTGAAATTATCAAGGGGATTGCTCGTGGAATTCTGTATCTTCACCAAGACTCAAGATTGAGGATTATCCATAGAGATCTAAAAACCAGCAATGTTCTTCTAGATGCTGAAATGAACCCAAAAATTTCCGACTTTGGCATGGCAAGAATATTTGATGGCAACCAAATTCAGAATAAAACGAACCGAGTTGTTGGAACATATGGCTACGTGTCACCAGAGTATGCAGTGTTTGGCAGATTTTCTGCTAAATCAGATGTCTTTAGTTTTGGGATCATATTGTTAGAGGTTGTATCTGGCAAAAGAAACAATGGTTTTTCTCAAGAGGATCCTTCCATGAACTTGATAAGACATGTTTGGGACCTCTGGAGAGAAGACAGAGCCTTAGAAATTGTGGATACGACTCTGGAGTCATATAATTCTGATGAGGTCATGAGATGCATTCAAGTTGCACTCTTGTGCGTGCAAGAAGAATCAGAGGACCGGCCAGCCATGTCTGCCATTATTTTCATGTTGAGTGGTGAAACATCTGCTCCATCTCCTAACCAACCTGCATATGTCTTCAGAAGAAACTCCAGCACTGATGCTGAATCATCAGTACCAAAACGATTATATTCTATAAACGACTTGACAATAACTGCAATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

810

Amino Acids

91.94

Weight (kDa)

5.59

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 182 4e-34 D-mannose binding lectin
S_locus_glycop PF00954 232 - 307 2.1e-12 S-locus glycoprotein domain
PAN_2 PF08276 331 - 397 1.6e-16 PAN-like domain
Pkinase PF00069 495 - 758 3.6e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 497 - 761 1.1e-49 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 768 - 810 4.5e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1527
AasI GACNNNNNNGTC 2 cut(s) 110, 2185
Acc36I ACCTGC 1 cut(s) 2335
AccB1I GGYRCC 1 cut(s) 848
AccI GTMKAC 1 cut(s) 335
AccII CGCG 1 cut(s) 349
AciI CCGC 5 cut(s) 349, 1122, 1400, 1642, 1753
AclWI GGATC 5 cut(s) 650, 1242, 2057, 2099, 2112
AcoI YGGCCR 2 cut(s) 1501, 2261
AcsI RAATTY 8 cut(s) 157, 406, 803, 1388, 1601, 1809, 1902, 1944
AcuI CTGAAG 3 cut(s) 130, 1617, 2323
AfaI GTAC 3 cut(s) 1172, 1335, 2376
AfiI CCNNNNNNNGG 5 cut(s) 66, 566, 567, 665, 1359
AflII CTTAAG 1 cut(s) 1102
AflIII ACRYGT 3 cut(s) 342, 1986, 2128
AhlI ACTAGT 1 cut(s) 292
AjnI CCWGG 6 cut(s) 50, 420, 658, 664, 904, 1698
AjuI GAANNNNNNNTTGG 2 cut(s) 1706, 1738
AleI CACNNNNGTG 1 cut(s) 2233
AluBI AGCT 5 cut(s) 391, 1250, 1559, 1722, 2426
AluI AGCT 5 cut(s) 391, 1250, 1559, 1722, 2426
Alw21I GWGCWC 3 cut(s) 270, 323, 1321
Alw26I GTCTC 3 cut(s) 96, 118, 1417
AlwI GGATC 5 cut(s) 650, 1242, 2057, 2099, 2112
AoxI GGCC 4 cut(s) 662, 668, 1501, 2261
ApeKI GCWGC 1 cut(s) 1466
ApoI RAATTY 8 cut(s) 157, 406, 803, 1388, 1601, 1809, 1902, 1944
Asp700I GAANNNNTTC 1 cut(s) 1111
AspLEI GCGC 1 cut(s) 1352
AspS9I GGNCC 5 cut(s) 662, 668, 1755, 2137, 2257
AsuC2I CCSGG 3 cut(s) 153, 561, 823
AsuHPI GGTGA 3 cut(s) 1814, 1983, 2309
AsuNHI GCTAGC 1 cut(s) 1324
AvaII GGWCC 3 cut(s) 1755, 2137, 2257
BaeI ACNNNNGTAYC 2 cut(s) 186, 219
BalI TGGCCA 1 cut(s) 1503
BamHI GGATCC 1 cut(s) 2104
BanI GGYRCC 1 cut(s) 848
BauI CACGAG 2 cut(s) 1357, 1803
BbsI GAAGAC 2 cut(s) 2157, 2329
Bbv12I GWGCWC 3 cut(s) 270, 323, 1321
BbvI GCAGC 1 cut(s) 1478
BccI CCATC 8 cut(s) 92, 254, 773, 998, 1139, 1177, 1928, 2320
BcgI CGANNNNNNTGC 2 cut(s) 1041, 1075
BciT130I CCWGG 6 cut(s) 52, 422, 660, 666, 906, 1700
BciVI GTATCC 1 cut(s) 2167
BclI TGATCA 1 cut(s) 1741
BcnI CCSGG 3 cut(s) 153, 561, 823
BcoDI GTCTC 3 cut(s) 96, 118, 1417
BcuI ACTAGT 1 cut(s) 292
BfaI CTAG 9 cut(s) 162, 198, 293, 521, 672, 918, 1260, 1325, 1880
BfrI CTTAAG 1 cut(s) 1102
BfuAI ACCTGC 1 cut(s) 2335
BfuI GTATCC 1 cut(s) 2167
BglII AGATCT 3 cut(s) 760, 1216, 1855
BisI GCNGC 1 cut(s) 1467
BlsI GCNGC 1 cut(s) 1468
BmcAI AGTACT 1 cut(s) 1335
Bme1390I CCNGG 9 cut(s) 52, 153, 422, 561, 660, 666, 823, 906, 1700
Bme18I GGWCC 3 cut(s) 1755, 2137, 2257
BmgT120I GGNCC 5 cut(s) 662, 668, 1755, 2137, 2257
BmiI GGNNCC 3 cut(s) 850, 2106, 2138
BmrFI CCNGG 9 cut(s) 52, 153, 422, 561, 660, 666, 823, 906, 1700
BmrI ACTGGG 2 cut(s) 525, 576
BmsI GCATC 6 cut(s) 712, 1052, 1061, 1873, 2204, 2350
BmtI GCTAGC 1 cut(s) 1328
BmuI ACTGGG 2 cut(s) 525, 576
BpiI GAAGAC 2 cut(s) 2157, 2329
BpmI CTGGAG 4 cut(s) 1430, 2164, 2204, 2335
BpuEI CTTGAG 2 cut(s) 1816, 2082
BpuMI CCSGG 3 cut(s) 153, 561, 823
Bsa29I ATCGAT 1 cut(s) 1046
BsaAI YACGTR 1 cut(s) 1987
BsaBI GATNNNNATC 1 cut(s) 936
BsaJI CCNNGG 7 cut(s) 51, 151, 559, 659, 665, 905, 1504
BsaXI ACNNNNNCTCC 4 cut(s) 279, 309, 2293, 2323
Bsc4I CCNNNNNNNGG 5 cut(s) 66, 566, 567, 665, 1359
Bse118I RCCGGY 1 cut(s) 2259
Bse1I ACTGG 5 cut(s) 173, 520, 571, 836, 1413
Bse3DI GCAATG 2 cut(s) 1876, 2424
Bse8I GATNNNNATC 1 cut(s) 936
BseBI CCWGG 6 cut(s) 52, 422, 660, 666, 906, 1700
BseCI ATCGAT 1 cut(s) 1046
BseDI CCNNGG 7 cut(s) 51, 151, 559, 659, 665, 905, 1504
BseGI GGATG 5 cut(s) 94, 430, 965, 1408, 1695
BseJI GATNNNNATC 1 cut(s) 936
BseLI CCNNNNNNNGG 5 cut(s) 66, 566, 567, 665, 1359
BseMI GCAATG 2 cut(s) 1876, 2424
BseMII CTCAG 3 cut(s) 206, 744, 1242
BseNI ACTGG 5 cut(s) 173, 520, 571, 836, 1413
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 1 cut(s) 1478
BsgI GTGCAG 3 cut(s) 28, 369, 795
Bsh1236I CGCG 1 cut(s) 349
Bsh1285I CGRYCG 2 cut(s) 353, 1061
BshFI GGCC 4 cut(s) 664, 670, 1503, 2263
BshNI GGYRCC 1 cut(s) 848
BshVI ATCGAT 1 cut(s) 1046
BsiEI CGRYCG 2 cut(s) 353, 1061
BsiHKAI GWGCWC 3 cut(s) 270, 323, 1321
BsiSI CCGG 4 cut(s) 153, 561, 823, 2260
BslFI GGGAC 5 cut(s) 68, 584, 805, 1606, 2150
BslI CCNNNNNNNGG 5 cut(s) 66, 566, 567, 665, 1359
BsmAI GTCTC 3 cut(s) 96, 118, 1417
BsmFI GGGAC 5 cut(s) 68, 584, 805, 1606, 2150
BsmI GAATGC 2 cut(s) 1261, 2217
BsnI GGCC 4 cut(s) 664, 670, 1503, 2263
Bsp1286I GDGCHC 3 cut(s) 270, 323, 1321
Bsp143I GATC 9 cut(s) 595, 655, 760, 1216, 1234, 1741, 1855, 2049, 2104
BspACI CCGC 5 cut(s) 349, 1122, 1400, 1642, 1753
BspANI GGCC 4 cut(s) 664, 670, 1503, 2263
BspCNI CTCAG 3 cut(s) 205, 745, 1243
BspDI ATCGAT 1 cut(s) 1046
BspFNI CGCG 1 cut(s) 349
BspHI TCATGA 1 cut(s) 2208
BspLI GGNNCC 3 cut(s) 850, 2106, 2138
BspMI ACCTGC 1 cut(s) 2335
BspOI GCTAGC 1 cut(s) 1328
BspPI GGATC 5 cut(s) 650, 1242, 2057, 2099, 2112
BspQI GCTCTTC 1 cut(s) 311
BspT107I GGYRCC 1 cut(s) 848
BspTI CTTAAG 1 cut(s) 1102
BsrDI GCAATG 2 cut(s) 1876, 2424
BsrFI RCCGGY 1 cut(s) 2259
BsrI ACTGG 5 cut(s) 173, 520, 571, 836, 1413
BssAI RCCGGY 1 cut(s) 2259
BssECI CCNNGG 7 cut(s) 51, 151, 559, 659, 665, 905, 1504
BssMI GATC 9 cut(s) 595, 655, 760, 1216, 1234, 1741, 1855, 2049, 2104
BssSI CACGAG 2 cut(s) 1357, 1803
BssT1I CCWWGG 1 cut(s) 1504
Bst2BI CACGAG 2 cut(s) 1357, 1803
Bst2UI CCWGG 6 cut(s) 52, 422, 660, 666, 906, 1700
Bst4CI ACNGT 4 cut(s) 217, 843, 899, 1059
Bst6I CTCTTC 3 cut(s) 29, 311, 1207
BstAFI CTTAAG 1 cut(s) 1102
BstBAI YACGTR 1 cut(s) 1987
BstC8I GCNNGC 5 cut(s) 1299, 1326, 1354, 2240, 2265
BstDEI CTNAG 5 cut(s) 192, 753, 1251, 1660, 2161
BstF5I GGATG 5 cut(s) 94, 430, 965, 1408, 1695
BstFNI CGCG 1 cut(s) 349
BstHHI GCGC 1 cut(s) 1352
BstKTI GATC 9 cut(s) 598, 658, 763, 1219, 1237, 1744, 1858, 2052, 2107
BstMAI GTCTC 3 cut(s) 96, 118, 1417
BstMBI GATC 9 cut(s) 595, 655, 760, 1216, 1234, 1741, 1855, 2049, 2104
BstMCI CGRYCG 2 cut(s) 353, 1061
BstMWI GCNNNNNNNGC 6 cut(s) 1049, 1256, 1325, 1625, 2273, 2423
BstNI CCWGG 6 cut(s) 52, 422, 660, 666, 906, 1700
BstNSI RCATGY 3 cut(s) 346, 1711, 2132
BstSCI CCNGG 9 cut(s) 50, 151, 420, 559, 658, 664, 821, 904, 1698
BstUI CGCG 1 cut(s) 349
BstV1I GCAGC 1 cut(s) 1478
BstV2I GAAGAC 2 cut(s) 2157, 2329
BstX2I RGATCY 4 cut(s) 760, 1216, 1855, 2104
BstYI RGATCY 4 cut(s) 760, 1216, 1855, 2104
Bsu15I ATCGAT 1 cut(s) 1046
BsuI GTATCC 1 cut(s) 2167
BsuRI GGCC 4 cut(s) 664, 670, 1503, 2263
BsuTUI ATCGAT 1 cut(s) 1046
BtsCI GGATG 5 cut(s) 94, 430, 965, 1408, 1695
BtsI GCAGTG 2 cut(s) 14, 2010
BtsIMutI CAGTG 5 cut(s) 14, 222, 848, 2010, 2355
BveI ACCTGC 1 cut(s) 2335
Cac8I GCNNGC 5 cut(s) 1299, 1326, 1354, 2240, 2265
CciI TCATGA 1 cut(s) 2208
CfoI GCGC 1 cut(s) 1352
Cfr10I RCCGGY 1 cut(s) 2259
Cfr13I GGNCC 5 cut(s) 662, 668, 1755, 2137, 2257
ClaI ATCGAT 1 cut(s) 1046
CseI GACGC 1 cut(s) 1049
Csp6I GTAC 3 cut(s) 1171, 1334, 2375
CviQI GTAC 3 cut(s) 1171, 1334, 2375
DdeI CTNAG 5 cut(s) 192, 753, 1251, 1660, 2161
DpnI GATC 9 cut(s) 597, 657, 762, 1218, 1236, 1743, 1857, 2051, 2106
DpnII GATC 9 cut(s) 595, 655, 760, 1216, 1234, 1741, 1855, 2049, 2104
DraI TTTAAA 1 cut(s) 1606
DrdI GACNNNNNNGTC 2 cut(s) 110, 2185
DseDI GACNNNNNNGTC 2 cut(s) 110, 2185
EaeI YGGCCR 2 cut(s) 1501, 2261
Eam1104I CTCTTC 3 cut(s) 29, 311, 1207
EarI CTCTTC 3 cut(s) 29, 311, 1207
EciI GGCGGA 1 cut(s) 1415
Eco130I CCWWGG 1 cut(s) 1504
Eco47I GGWCC 3 cut(s) 1755, 2137, 2257
Eco57I CTGAAG 3 cut(s) 130, 1617, 2323
EcoO109I RGGNCCY 2 cut(s) 668, 2137
EcoRI GAATTC 1 cut(s) 1809
EcoRII CCWGG 6 cut(s) 50, 420, 658, 664, 904, 1698
EcoT14I CCWWGG 1 cut(s) 1504
EcoT22I ATGCAT 1 cut(s) 2219
ErhI CCWWGG 1 cut(s) 1504
FalI AAGNNNNNCTT 3 cut(s) 35, 1605, 1637
FaqI GGGAC 5 cut(s) 68, 584, 805, 1606, 2150
FauNDI CATATG 2 cut(s) 1978, 2332
FbaI TGATCA 1 cut(s) 1741
FblI GTMKAC 1 cut(s) 335
Fnu4HI GCNGC 1 cut(s) 1467
FokI GGATG 5 cut(s) 101, 437, 972, 1415, 1702
Fsp4HI GCNGC 1 cut(s) 1467
FspBI CTAG 9 cut(s) 162, 198, 293, 521, 672, 918, 1260, 1325, 1880
GlaI GCGC 1 cut(s) 1351
GluI GCNGC 1 cut(s) 1467
GsuI CTGGAG 4 cut(s) 1430, 2164, 2204, 2335
HaeIII GGCC 4 cut(s) 664, 670, 1503, 2263
HapII CCGG 4 cut(s) 153, 561, 823, 2260
HgaI GACGC 1 cut(s) 1049
HhaI GCGC 1 cut(s) 1352
Hin6I GCGC 1 cut(s) 1350
HinP1I GCGC 1 cut(s) 1350
HincII GTYRAC 2 cut(s) 367, 744
HindII GTYRAC 2 cut(s) 367, 744
HindIII AAGCTT 1 cut(s) 1720
HpaI GTTAAC 1 cut(s) 367
HpaII CCGG 4 cut(s) 153, 561, 823, 2260
HphI GGTGA 3 cut(s) 1814, 1983, 2309
Hpy166II GTNNAC 5 cut(s) 336, 367, 744, 902, 1200
Hpy8I GTNNAC 5 cut(s) 336, 367, 744, 902, 1200
HpyAV CCTTC 6 cut(s) 79, 797, 1281, 1743, 1768, 2118
HpyCH4III ACNGT 4 cut(s) 217, 843, 899, 1059
HpyCH4IV ACGT 2 cut(s) 740, 1986
HpyF10VI GCNNNNNNNGC 6 cut(s) 1049, 1256, 1325, 1625, 2273, 2423
HpyF3I CTNAG 5 cut(s) 192, 753, 1251, 1660, 2161
HpySE526I ACGT 2 cut(s) 740, 1986
HspAI GCGC 1 cut(s) 1350
Ksp22I TGATCA 1 cut(s) 1741
KspAI GTTAAC 1 cut(s) 367
Kzo9I GATC 9 cut(s) 595, 655, 760, 1216, 1234, 1741, 1855, 2049, 2104
LguI GCTCTTC 1 cut(s) 311
LmnI GCTCC 1 cut(s) 2314
Lsp1109I GCAGC 1 cut(s) 1478
LweI GCATC 6 cut(s) 712, 1052, 1061, 1873, 2204, 2350
MaeI CTAG 9 cut(s) 162, 198, 293, 521, 672, 918, 1260, 1325, 1880
MaeII ACGT 2 cut(s) 740, 1986
MaeIII GTNAC 5 cut(s) 546, 679, 827, 1536, 1989
MalI GATC 9 cut(s) 597, 657, 762, 1218, 1236, 1743, 1857, 2051, 2106
MboI GATC 9 cut(s) 595, 655, 760, 1216, 1234, 1741, 1855, 2049, 2104
MfeI CAATTG 1 cut(s) 80
MflI RGATCY 4 cut(s) 760, 1216, 1855, 2104
MhlI GDGCHC 3 cut(s) 270, 323, 1321
MlsI TGGCCA 1 cut(s) 1503
MluNI TGGCCA 1 cut(s) 1503
MlyI GAGTC 4 cut(s) 1721, 1823, 2173, 2195
MmeI TCCRAC 5 cut(s) 157, 478, 491, 1932, 1951
Mox20I TGGCCA 1 cut(s) 1503
Mph1103I ATGCAT 1 cut(s) 2219
MroXI GAANNNNTTC 1 cut(s) 1111
MscI TGGCCA 1 cut(s) 1503
MseI TTAA 7 cut(s) 366, 533, 879, 1014, 1029, 1103, 1605
MslI CAYNNNNRTG 3 cut(s) 1460, 1491, 2233
Msp20I TGGCCA 1 cut(s) 1503
MspCI CTTAAG 1 cut(s) 1102
MspI CCGG 4 cut(s) 153, 561, 823, 2260
MspR9I CCNGG 9 cut(s) 52, 153, 422, 561, 660, 666, 823, 906, 1700
MunI CAATTG 1 cut(s) 80
Mva1269I GAATGC 2 cut(s) 1261, 2217
MvaI CCWGG 6 cut(s) 52, 422, 660, 666, 906, 1700
MvnI CGCG 1 cut(s) 349
MwoI GCNNNNNNNGC 6 cut(s) 1049, 1256, 1325, 1625, 2273, 2423
NciI CCSGG 3 cut(s) 153, 561, 823
NdeI CATATG 2 cut(s) 1978, 2332
NdeII GATC 9 cut(s) 595, 655, 760, 1216, 1234, 1741, 1855, 2049, 2104
NheI GCTAGC 1 cut(s) 1324
NlaIV GGNNCC 3 cut(s) 850, 2106, 2138
NmuCI GTSAC 4 cut(s) 546, 679, 827, 1989
NsiI ATGCAT 1 cut(s) 2219
NspI RCATGY 3 cut(s) 346, 1711, 2132
OliI CACNNNNGTG 1 cut(s) 2233
PagI TCATGA 1 cut(s) 2208
PciI ACATGT 2 cut(s) 342, 2128
PciSI GCTCTTC 1 cut(s) 311
PctI GAATGC 2 cut(s) 1261, 2217
PdmI GAANNNNTTC 1 cut(s) 1111
PfeI GAWTC 6 cut(s) 180, 438, 749, 937, 2249, 2366
PfoI TCCNGGA 2 cut(s) 420, 1698
PkrI GCNGC 1 cut(s) 1468
PleI GAGTC 4 cut(s) 1721, 1823, 2173, 2194
PpsI GAGTC 4 cut(s) 1721, 1823, 2173, 2194
Ppu21I YACGTR 1 cut(s) 1987
PpuMI RGGWCCY 1 cut(s) 2137
PscI ACATGT 2 cut(s) 342, 2128
PsiI TTATAA 1 cut(s) 1527
Psp5II RGGWCCY 1 cut(s) 2137
Psp6I CCWGG 6 cut(s) 50, 420, 658, 664, 904, 1698
PspGI CCWGG 6 cut(s) 50, 420, 658, 664, 904, 1698
PspN4I GGNNCC 3 cut(s) 850, 2106, 2138
PspPI GGNCC 5 cut(s) 662, 668, 1755, 2137, 2257
PspPPI RGGWCCY 1 cut(s) 2137
PsuI RGATCY 4 cut(s) 760, 1216, 1855, 2104
RsaI GTAC 3 cut(s) 1172, 1335, 2376
RsaNI GTAC 3 cut(s) 1171, 1334, 2375
RseI CAYNNNNRTG 3 cut(s) 1460, 1491, 2233
SapI GCTCTTC 1 cut(s) 311
SaqAI TTAA 7 cut(s) 366, 533, 879, 1014, 1029, 1103, 1605
SatI GCNGC 1 cut(s) 1467
Sau3AI GATC 9 cut(s) 595, 655, 760, 1216, 1234, 1741, 1855, 2049, 2104
Sau96I GGNCC 5 cut(s) 662, 668, 1755, 2137, 2257
ScaI AGTACT 1 cut(s) 1335
SchI GAGTC 4 cut(s) 1721, 1823, 2173, 2195
ScrFI CCNGG 9 cut(s) 52, 153, 422, 561, 660, 666, 823, 906, 1700
SduI GDGCHC 3 cut(s) 270, 323, 1321
SfaNI GCATC 6 cut(s) 712, 1052, 1061, 1873, 2204, 2350
SinI GGWCC 3 cut(s) 1755, 2137, 2257
SmiMI CAYNNNNRTG 3 cut(s) 1460, 1491, 2233
SmlI CTYRAG 3 cut(s) 1102, 1831, 2097
SmoI CTYRAG 3 cut(s) 1102, 1831, 2097
SpeI ACTAGT 1 cut(s) 292
SsiI CCGC 5 cut(s) 349, 1122, 1400, 1642, 1753
SspI AATATT 3 cut(s) 617, 644, 1929
SspMI CTAG 9 cut(s) 162, 198, 293, 521, 672, 918, 1260, 1325, 1880
StyD4I CCNGG 9 cut(s) 50, 151, 420, 559, 658, 664, 821, 904, 1698
StyI CCWWGG 1 cut(s) 1504
TaaI ACNGT 4 cut(s) 217, 843, 899, 1059
TaiI ACGT 2 cut(s) 743, 1989
TaqI TCGA 4 cut(s) 1046, 1061, 1242, 2428
TatI WGTACW 1 cut(s) 1333
TfiI GAWTC 6 cut(s) 180, 438, 749, 937, 2249, 2366
Tru1I TTAA 7 cut(s) 366, 533, 879, 1014, 1029, 1103, 1605
Tru9I TTAA 7 cut(s) 366, 533, 879, 1014, 1029, 1103, 1605
TscAI CASTG 5 cut(s) 21, 222, 848, 2010, 2362
TseFI GTSAC 4 cut(s) 546, 679, 827, 1989
TseI GCWGC 1 cut(s) 1466
Tsp45I GTSAC 4 cut(s) 546, 679, 827, 1989
TspGWI ACGGA 2 cut(s) 1400, 1702
TspRI CASTG 5 cut(s) 21, 222, 848, 2010, 2362
Vha464I CTTAAG 1 cut(s) 1102
VpaK11BI GGWCC 3 cut(s) 1755, 2137, 2257
XapI RAATTY 8 cut(s) 157, 406, 803, 1388, 1601, 1809, 1902, 1944
XbaI TCTAGA 1 cut(s) 1879
XceI RCATGY 3 cut(s) 346, 1711, 2132
XcmI CCANNNNNNNNNTGG 1 cut(s) 1511
XmiI GTMKAC 1 cut(s) 335
XmnI GAANNNNTTC 1 cut(s) 1111
XspI CTAG 9 cut(s) 162, 198, 293, 521, 672, 918, 1260, 1325, 1880
ZrmI AGTACT 1 cut(s) 1335
Zsp2I ATGCAT 1 cut(s) 2219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.